CN114099584A - Antiviral and antibacterial traditional Chinese medicine composition containing houttuynia cordata, and preparation method and application thereof - Google Patents
Antiviral and antibacterial traditional Chinese medicine composition containing houttuynia cordata, and preparation method and application thereof Download PDFInfo
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- CN114099584A CN114099584A CN202010884909.3A CN202010884909A CN114099584A CN 114099584 A CN114099584 A CN 114099584A CN 202010884909 A CN202010884909 A CN 202010884909A CN 114099584 A CN114099584 A CN 114099584A
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- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation or decoction
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Abstract
The invention relates to the technical field of traditional Chinese medicines, in particular to an antiviral and antibacterial traditional Chinese medicine composition containing houttuynia cordata, and a preparation method and application thereof. The traditional Chinese medicine composition comprises the following raw materials: honeysuckle, houttuynia cordata, wrinkled gianthyssop herb, gypsum, ephedra herb, menthol, rhubarb, bitter apricot kernel and liquorice. The antiviral composition can quickly relieve and effectively cure the symptoms of fever and itching, hidden rubella, mouth and tongue sores, swollen and sore throat, dry mouth and thirst, dry cough, urgent breath, white phlegm with little blood, dry stool, thin and yellow tongue coating, bright red tongue quality and floating and rapid pulse caused by wind heat and damp toxin, and animal experiments and antibacterial experiments prove that the antibacterial and antiviral traditional Chinese medicine composition has obvious functions of resisting virus, bacteria, relieving cough and allaying fever, so that the antibacterial and antiviral traditional Chinese medicine composition can be prepared into medicines for dissipating wind heat and eliminating damp toxin and antiviral and antibacterial medicines.
Description
Technical Field
The invention relates to the technical field of traditional Chinese medicines, in particular to an antiviral and antibacterial traditional Chinese medicine composition containing houttuynia cordata, and a preparation method and application thereof.
Background
Summer heat is exuberant, the metabolism of the human body is vigorous, the internal heat in intestines and stomach can be accumulated when people stay up or work and rest disorderly, and the internal heat is mixed with external high-temperature external heat, so that the internal heat is easy to cause damage to the skin, form rubella, and cause the symptoms of excessive internal heat such as constipation, oral ulcer, pharyngalgia and the like. Meanwhile, various aquatic products come into the market in summer, and when the aquatic products are eaten and allergic, incoordination between the spleen and the stomach can be caused, dampness and heat are accumulated, skin itch can be caused, and rubella can appear. For people with lower immunity, a series of exterior syndromes caused by wind-heat attacking the exterior and lung defense are more likely to occur.
On the other hand, people easily enjoy cool in summer, but the moisture of the body is aggravated easily when the people blow air or eat cold food or drink cold water for a long time. Therefore, people suffering from wind-heat attack in summer often coexist with damp toxin.
The western medicine adopts symptomatic treatment aiming at the symptoms, usually can only treat the symptoms, but not the root causes, or can only improve a part of symptoms, but can not completely eliminate all symptoms, so the treatment effect is not good.
Disclosure of Invention
The invention provides an antiviral and antibacterial traditional Chinese medicine composition containing houttuynia cordata, and a preparation method and application thereof, aiming at the technical problem that the effect of western medicines for treating patients affected by wind heat and accompanied with damp toxin is poor at present.
In order to achieve the purpose of the invention, the embodiment of the invention adopts the following technical scheme:
on one hand, the embodiment of the invention provides an antibacterial and antiviral traditional Chinese medicine composition which comprises the following raw materials in parts by weight: 8-13 parts of honeysuckle, 7-11 parts of houttuynia cordata, 7-11 parts of wrinkled gianthyssop herb, 12-18 parts of gypsum, 4-8 parts of ephedra, 2-4 parts of menthol, 4-8 parts of rheum officinale, 7-11 parts of bitter almond and 2-4 parts of liquorice.
The antibacterial and antiviral traditional Chinese medicine composition provided by the invention comprises the following components:
honeysuckle flower, flos Lonicerae, with cold nature, enters lung, heart, stomach and large intestine meridians, excels in dispersing lung meridian wind-heat, clearing heart and lung heat-toxin, has the property of dispersing and dispelling wind, has strong ability of clearing heat-toxin, detumescence and dissipating nodulation, and can cool blood and stop dysentery.
Yu xing Cao is slightly cold in nature, enters lung meridian, excels in clearing lung heat, excels in treating pulmonary abscess, and can clear heat and remove toxicity, and induce diuresis to treat stranguria.
Agastache rugosus has mild warm property, enters spleen, stomach and lung meridians, has warm nature and is not dry, can not only harmonize the middle energizer and damp-turbidity of spleen and stomach, but also harmonize the middle warmer and arrest vomiting, and can disperse exterior cold.
Gypsum, being cold in nature, enters lung and stomach meridians, and can both transmit heat to the exterior and release heat to the interior, is the essential herb for clearing lung heat and purging stomach fire, and also has the action of astringing dampness.
The ephedra herb is warm in nature, enters lung and bladder channels, has light weight and floats upwards, can disperse lung qi, open striae and clear sweat pores, dispel wind-cold, relieve exterior syndrome by sweating, and also has the effects of inducing diuresis and relieving swelling.
Menthol is cool in nature, enters lung and liver meridians, disperses wind and clears heat, clears head and eyes, relieves sore throat and promotes eruption, soothes liver and promotes qi circulation.
Rhubarb, being cold in nature, enters spleen, stomach, large intestine, liver and pericardium channels, has the property of sinking, can lead heat to descend, has strong purgative effect, can purge and eliminate accumulation, wash intestines and stomach, and clear heat of blood components, and has the effects of cooling blood, removing toxicity, activating blood and removing stasis.
Bitter apricot kernel, semen Armeniacae amarum, being slightly warm in nature, enters lung and large intestine meridians, has the effects of relieving cough and asthma by bitter discharge and descending qi, is rich in oil, and can moisten intestine and relax bowel.
The liquorice has neutral nature, enters heart, spleen, lung and stomach channels, tonifies spleen and qi, moistens lung and stops cough, clears away heat and toxic material, relieves pain and relieves medicine property.
In the antibacterial and antiviral traditional Chinese medicine composition, the gypsum is pungent, sweet and cold, clears lung heat and promotes fluid production to relieve cough; honeysuckle flower has the effects of warming body, aphtha, sore throat, detumescence and detoxification; rhubarb, radix et rhizoma Rhei clears the abdomen and purges heat, treats dry stool, combines with gypsum, disperses and degrades from top to bottom, and clears away heat-toxin in lung and stomach. The combination of the three drugs, exterior and interior, is the monarch drug. The ephedra herb is matched with the gypsum to disperse wind heat in the lung and clear away stagnated heat; the five-ingredient menthol can clear and relieve the throat and disperse the heat of the upper-jiao; with the addition of houttuynia cordata, the medicine can clear away heat from the lung. The three medicines are used as ministerial medicines. The assistant almond prevents the ephedra herb and the gypsum from spreading too much, the wrinkled gianthyssop herb prevents the cold and cool from hurting the spleen, and the liquorice harmonizes the other drugs to moderate the property of the drugs. The three medicines are used as adjuvant medicines.
Clinical application effects prove that the whole formula can quickly relieve and effectively cure the symptoms of fever, itch, hidden rubella, aphtha of the mouth and the tongue, sore throat, dry mouth, thirst, dry cough, shortness of breath, white phlegm, little blood streak, dry stool, thin and yellow tongue fur, bright red tongue texture and floating and rapid pulse through the compatibility of the medicines. Animal experiments and bacteriostatic experiments prove that the antibacterial and antiviral traditional Chinese medicine composition has remarkable antiviral, antibacterial, cough relieving and fever reducing effects.
Preferably, the antibacterial and antiviral traditional Chinese medicine composition comprises the following raw materials in parts by weight: 9-12 parts of honeysuckle, 8-10 parts of houttuynia cordata, 8-10 parts of wrinkled gianthyssop herb, 13-17 parts of gypsum, 5-7 parts of ephedra, 3-4 parts of menthol, 5-7 parts of rheum officinale, 8-10 parts of bitter almond and 3-4 parts of liquorice.
Preferably, the antibacterial and antiviral traditional Chinese medicine composition comprises the following raw materials in parts by weight: 10 parts of honeysuckle, 9 parts of houttuynia cordata, 9 parts of agastache rugosus, 15 parts of gypsum, 6 parts of ephedra, 3 parts of menthol, 6 parts of rheum officinale, 9 parts of bitter almond and 3 parts of liquorice.
In the practical use of the traditional Chinese medicine, 1g of the traditional Chinese medicine is used as each dose, and 1-2 doses are used every day according to the state of illness.
In a second aspect, the embodiment of the present invention further provides a preparation method of the antibacterial and antiviral traditional Chinese medicine composition, including the following operations: weighing the raw materials according to the raw material proportion in the antibacterial and antiviral traditional Chinese medicine composition, extracting volatile oil of the agastache rugosus, decocting residues with the honeysuckle, the houttuynia cordata, the gypsum, the ephedra, the rheum officinale and the bitter apricot seeds in water, filtering, collecting obtained filtrate, and drying to obtain a water-decocted extract; and adding the volatile oil of the agastache rugosus into the obtained water decoction extract, uniformly mixing, adding the menthol, and uniformly mixing to obtain the antibacterial antiviral traditional Chinese medicine composition. The product prepared by the preparation method can be further processed into oral dosage forms such as tablets, granules, powder, capsules, syrup, suspension and the like according to the actual needs of clinical use or dosage form requirements.
Wherein, the water-decocted extract can be prepared by the following operation processes: decocting the gypsum for 15-25 min, adding the residue obtained after the volatile oil is extracted from the agastache rugosus and the honeysuckle, the houttuynia cordata, the ephedra, the bitter apricot kernel and the liquorice, decocting, adding the rheum officinale, continuing to decoct for 5-10 min, filtering to obtain a water-decocted filtrate, and drying the water-decocted filtrate to obtain a water-decocted extract. The water amount of the gypsum can be 6-9 times of the total weight of the gypsum, the honeysuckle, the houttuynia cordata, the ephedra, the bitter apricot kernel and the liquorice, and the honeysuckle, the houttuynia cordata, the ephedra, the bitter apricot kernel and the liquorice are directly added to be decocted for 2-3 times, 20-35 min is needed each time, and 2 times and 30min is needed each time. The time for decocting is preferably 8min after adding radix et rhizoma Rhei.
The drying method in the preparation method can adopt a spray drying or decompression drying method at 45-55 ℃, or freeze drying, and before drying, decompression concentration at 45-55 ℃ can be carried out.
In a third aspect, the embodiment of the invention also provides application of the antibacterial and antiviral traditional Chinese medicine composition in preparation of a medicine for relieving itching and eliminating rash.
In a fourth aspect, the embodiment of the invention also provides application of the antibacterial and antiviral traditional Chinese medicine composition in preparing a medicine for treating aphtha of the mouth and tongue.
In a fifth aspect, the embodiment of the invention also provides application of the antibacterial and antiviral traditional Chinese medicine composition in preparing a medicine for treating sore throat.
In a sixth aspect, the embodiment of the invention also provides application of the antibacterial and antiviral traditional Chinese medicine composition in preparing a medicine for treating dry cough.
In a seventh aspect, the embodiment of the invention also provides application of the antibacterial and antiviral traditional Chinese medicine composition in preparation of medicines for treating at least two symptoms of rubella itching, aphtha of the mouth and tongue, sore throat, dry cough and dry stool.
Clinical verification shows that the antibacterial and antiviral traditional Chinese medicine composition has obvious treatment effects on symptoms of fever, pruritus, rubella, aphtha, sore throat, dry mouth, thirst, dry cough, shortness of breath, white phlegm, little blood streak, dry stool, thin and yellow tongue coating, bright red tongue and floating and rapid pulse caused by wind heat and damp toxin, so that the antibacterial and antiviral traditional Chinese medicine composition can be used for preparing a medicine for treating one or more symptoms.
In an eighth aspect, the embodiment of the present invention further provides an antiviral and/or antibacterial drug, which includes the above antibacterial and antiviral traditional Chinese medicine composition.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention. The experimental procedures used in the following examples are all conventional in the art unless otherwise specified.
The honeysuckle, the heartleaf houttuynia herb, the wrinkled gianthyssop herb, the gypsum, the ephedra herb, the menthol, the rhubarb, the bitter apricot kernel and the liquorice used in the following examples all conform to the relevant regulations under the terms of each medicinal material in the part of Chinese pharmacopoeia (2015 edition).
The raw materials, reagents and the like used in the following examples were obtained commercially unless otherwise specified.
Examples
The embodiment of the invention provides an antibacterial and antiviral traditional Chinese medicine composition, and the raw material composition of each dose is shown in table 1 (unit: g) in each embodiment.
TABLE 1 raw material composition of the antibacterial and antiviral Chinese medicinal composition of each example
| Raw materials | Example 1 | Example 2 | Example 3 | Example 4 | Example 5 |
| Honeysuckle | 10 | 9 | 12 | 8 | 13 |
| Herba Houttuyniae | 9 | 8 | 10 | 7 | 11 |
| Agastache rugosus | 9 | 8 | 10 | 7 | 11 |
| Gypsum plaster | 15 | 13 | 17 | 12 | 18 |
| Chinese ephedra | 6 | 5 | 7 | 4 | 8 |
| Menthol crystal | 3 | 3 | 4 | 2 | 4 |
| Radix et rhizoma Rhei | 6 | 5 | 7 | 4 | 8 |
| Bitter apricot kernel | 9 | 8 | 10 | 7 | 11 |
| Licorice root, radix Glycyrrhizae | 3 | 3 | 4 | 2 | 4 |
The embodiment of the invention also provides a preparation method of the antibacterial and antiviral traditional Chinese medicine composition in the embodiment, the raw materials are weighed according to the mixture ratio of the raw materials in the antibacterial and antiviral traditional Chinese medicine composition in the embodiment, the volatile oil of the agastache rugosus is extracted, the gypsum is decocted for 15-25 min, the residue after the volatile oil extraction of the agastache rugosus and the honeysuckle, the houttuynia cordata, the ephedra, the bitter almond and the liquorice are added and decocted for 2-3 times, and each time lasts for 20-35 min; then adding rhubarb and continuing to decoct for 5-10 min, filtering to obtain a water-decocted filtrate, and spray-drying the water-decocted filtrate at 45-55 ℃ to obtain a water-decocted extract; adding herba Agastaches volatile oil into the obtained water decoction extract, mixing, adding Mentholum, and mixing.
The specific parameters and conditions of the preparation process of each example are shown in table 2.
TABLE 2 preparation parameters of the examples
Comparative example 1
The invention provides an antibacterial and antiviral traditional Chinese medicine composition, which is based on the raw material composition of the embodiment 1, and the dosage of wrinkled gianthyssop which has similar efficacy with the ephedra and can release the exterior is increased by removing the ephedra. The raw materials in each dose are: 10g of honeysuckle, 9g of houttuynia cordata, 15g of wrinkled gianthyssop herb, 15g of gypsum, 3g of menthol, 6g of rhubarb, 9g of bitter almond and 3g of liquorice. The preparation method is the same as example 1.
Comparative example 2
The invention provides an antibacterial and antiviral traditional Chinese medicine composition, based on the raw material composition of example 1, houttuynia cordata is removed, and the using amount of honeysuckle which has similar effect to houttuynia cordata and can clear away heat and toxic materials is increased. The raw materials in each dose are: 19g of honeysuckle, 9g of wrinkled gianthyssop herb, 15g of gypsum, 6g of ephedra herb, 3g of menthol, 6g of rhubarb, 9g of bitter apricot seed and 3g of liquorice. The preparation method is the same as example 1.
Effect example 1
The effect example provides a mouse gavage single-administration toxicity test of the antibacterial and antiviral traditional Chinese medicine composition, so as to observe possible toxic reaction after the antibacterial and antiviral traditional Chinese medicine composition is taken.
1. Material
1.1 test drugs
The test solutions were prepared according to the methods and the raw materials of examples 1 to 5, respectively, by uniformly grinding, dissolving with 0.5% CMC-Na, and preparing a concentration of 2g crude drug/ml.
1.2 test animals
ICR mice, SPF grade, 240 mice, 20-22g, half male and half female, purchased from Beijing Wintonlihua laboratory animal technology Co., Ltd, license number: SCXK (Kyoto) 2016-.
2. Method of producing a composite material
2.1 grouping and administration
The male and female mice were randomly divided into 6 groups of 20 mice each, and each group was a blank control group and 5 example groups (example 1 group, example 2 group, example 3 group, example 4 group and example 5 group), each example group was gavaged with the test solutions of examples 1 to 5 obtained in 1.1, respectively, at a volume of 0.4ml/10g body weight (maximum volume), and were co-administered twice a day at 6-hour intervals, and the blank control group was administered with an equal amount of 0.5% CMC-Na. All animals were fasted overnight before dosing without water deprivation.
2.2 observed metrics, time and content
After each administration, the animals are continuously observed for 2 hours, and the animals are observed for physical signs, behaviors, secretions, excretions, death conditions, toxic reactions (symptoms, initial time, severity, duration and reversibility of toxic reactions) and the like, and the information of the appearance time, duration and the like of abnormal symptoms is recorded in detail. Animal body weight and food intake were recorded once a day on days 1, 2, 3, 4, 7, and 14 after dosing for 14 consecutive days. At the end of the observation period, the surviving animals were euthanized, gross dissected, and when changes in organ volume, color, texture, etc. were found, histopathological examination of the altered organs was performed.
2.3 data analysis
The test data adopts SPSS18.0 statistical software, and the results are mean plus or minus standard deviation
Indicated, independent sample two-sided T-test was used.
3. Results
3.1 Effect on general conditions
The blank control group and each example group have no abnormal general condition observation.
3.2 Effect on body weight and food intake
Compared with the blank control group, the weights of the female animals and the male animals in each example group are not obviously and statistically different; the food intake was not significantly different from that of the blank control group. See tables 3-6.
TABLE 3 ICR mice gavage Single dose toxicity test Male body weight Change
(g)
TABLE 4 weight change in female animals tested for toxicity in ICR mice by single gavage administration
(g)
TABLE 5 ICR mice gavage Single dose toxicity test Male intake Change
(g/day/only)
TABLE 6 ICR mice gavage Single dose toxicity test female animals intake Change
(g/day/only)
3.3 general dissection
After the observation period, the mice of the blank control group and the mice of the examples are roughly dissected, and no obvious abnormality is found in the heart, the liver, the spleen, the lung, the kidney, the brain, the stomach, the intestine, the thymus, the reproductive organs, the bladder and the like.
4. Conclusion
The maximum dose of the mouse gavage drug is 160g crude drug/kg, and no related toxic reaction of the test substance is found at the dose.
Effect example 2
The effect example provides an experiment on the influence of the antibacterial and antiviral traditional Chinese medicine composition on the fever of rats caused by LPS, so as to observe the antipyretic effect of taking the antibacterial and antiviral traditional Chinese medicine composition.
1. Material
1.1 test drugs
The test solutions were prepared according to the raw material compositions and preparation methods of examples 1 to 5 and comparative examples 1 and 2, respectively, by uniformly grinding, dissolving with 0.5% CMC-Na, and formulating to a concentration of 0.3g crude drug/ml as a test solution.
1.2 test animals
SD rat, SPF grade, 96, 180-: SCXK (Kyoto) 2016-.
2. Method of producing a composite material
2.1 grouping and administration
Rats were randomly divided into 8 groups of 12 rats each, which were a model control group, 5 example groups (example 1 group, example 2 group, example 3 group, example 4 group and example 5 group), a comparative example 1 group and a comparative example 2 group, respectively, each example group was gavaged with the test solutions of examples 1 to 5 obtained in 1.1, and each comparative example 1 group and comparative example 2 group was gavaged with the test solutions of comparative examples 1 and 2 obtained in 1.1, respectively, with a gavage volume of 1ml/100g body weight. The model control group was given an equivalent amount of 0.5% CMC-Na. 30min after each group of rats is dosed, anal temperature is measured once and recorded as T0Then, 20. mu.g/kg LPS (saline solution preparation) was intraperitoneally injected to make a mold, and the volume was 5 ml/kg. After the model is made, the anal temperature is measured every 1h and recorded as TxContinuously calculating the temperature difference delta T of the body for 5h, wherein the delta T is equal to Tx-T0。
2.2 data analysis
The test data adopts SPSS18.0 statistical software, and the results are mean plus or minus standard deviation
Showing that one-way analysis of variance is adopted.
3. Results
The temperature difference of the rat body of the model control group is obviously increased after 1-5h of intraperitoneal LPS (Low pressure) injection molding, compared with the model control group, the temperature difference of the rat body of the example group is obviously reduced after 2h and 3h of molding (P <0.05), and the temperature difference of the rat body of the comparative group is obviously reduced after 3h of molding (P < 0.05). See table 7.
TABLE 7 Effect on LPS induced fever in rats
Note: comparison with model control group: p < 0.05.
4. Conclusion
The antibacterial and antiviral traditional Chinese medicine composition provided by the invention has the effect of inhibiting fever caused by rat intraperitoneal injection of LPS, and the antibacterial and antiviral traditional Chinese medicine composition provided by the embodiment of the invention is superior to a comparative example.
Effect example 3
The effect example provides the influence of the antibacterial and antiviral traditional Chinese medicine composition on influenza virus pneumonia of mice so as to observe the antiviral effect of taking the antibacterial and antiviral traditional Chinese medicine composition.
1. Material
1.1 test drugs
The test solutions were prepared according to the raw material compositions and preparation methods of examples 1 to 5 and comparative examples 1 and 2, respectively, by uniformly grinding, dissolving with 0.5% CMC-Na, and formulating to a concentration of 0.4g crude drug/ml as a test solution.
1.2 test animals
KM mice, SPF grade, 108, 20-22g, male, purchased from experimental animal technology ltd, viton, beijing, license number: SCXK (Kyoto) 2016-.
1.3 viruses
Influenza virus mouse lung adapted strain (FM)1) It is introduced from virology research institute of Chinese academy of preventive medicine science, and is used by inoculating allantoic cavity of 9-day-old chick embryo, culturing at 37 deg.C for 3 days, and collecting allantoic fluid.
2. Method of producing a composite material
2.1 grouping and administration
The mice were randomly divided into 9 groups of 12 mice each, namely, a normal control group, a model control group, and 5 example groups (example 1 group, example 2 group, mouse group, and mouse group, and mouse group, and mouse group are divided into 12, and group, and mouse, and group,Example 3, example 4 and example 5 groups), comparative example 1 and comparative example 2 groups. The test solutions of examples 1 to 5 obtained in 1.1 were used for intragastric administration in each example group, and the test solutions of comparative examples 1 and 2 obtained in 1.1 were used for intragastric administration in comparative example 1 and comparative example 2, respectively, and were continuously administered by intragastric administration for 7 days, once a day, with a volume of 0.2ml/10g body weight. The normal control group and the model control group were given an equivalent amount of 0.5% CMC-Na. 30 minutes after the mice were dosed on day 3, 50 μ L LD under light ether anesthesia50The virus is dripped into the nose for infection, the normal control group is dripped into the nose by using sterile PBS liquid with the same volume, the mouse is dissected 96 hours after the infection, the lung is taken and weighed, and the lung index is calculated. Taking lung tissue, fixing with formaldehyde, staining by conventional HE, and observing pathological changes of the lung tissue with a light microscope.
2.2 data analysis
The test data adopts SPSS18.0 statistical software, and the results are mean plus or minus standard deviation
Showing that one-way analysis of variance is adopted.
3. Results
3.1 Effect on influenza Virus pulmonary index
The lung index of the model control group was significantly increased (P <0.05) compared to the normal control group, the lung index of the example group and the comparative example group was significantly decreased (P <0.05) compared to the model control group, and the lung index of the example group was lower than that of the comparative example group. See table 8.
TABLE 8 Effect on influenza Virus pulmonary index
Note: comparison with model control group: p < 0.05.
3.2 Effect of the inventive Agents on alterations in Lung tissue pathology
The pathological changes of the lung tissues of the mice are observed under a light microscope, the alveolar cavities of the model control group are full of blood exudates, a large amount of epithelia are shed, and the alveolar wall structure is unclear. The lesions were significantly reduced in the example group and the comparative example group compared to the model group. The lesion range of the example group was more limited than that of the comparative group, the inflammatory cell density was lower, the serous exudation in the alveolar space was less, and the alveolar structure was more intact.
4. Conclusion
The antibacterial and antiviral traditional Chinese medicine composition provided by the embodiment of the invention has an obvious inhibiting effect on mouse influenza virus pneumonia after being administrated in vivo, and is superior to a comparative example.
Effect example 4
The effect example provides the influence of the antibacterial and antiviral traditional Chinese medicine composition on the cough of a mouse caused by ammonia water spraying, so as to observe the cough relieving effect of taking the antibacterial and antiviral traditional Chinese medicine composition.
1. Material
1.1 test drugs
The test solutions were prepared according to the raw material compositions and preparation methods of examples 1 to 5 and comparative examples 1 and 2, respectively, by uniformly grinding, dissolving with 0.5% CMC-Na, and formulating to a concentration of 0.4g crude drug/ml as a test solution.
1.2 test animals
KM mice, SPF grade, 108, 20-22g, male, purchased from experimental animal technology ltd, viton, beijing, license number: SCXK (Kyoto) 2016-.
2. Method of producing a composite material
2.1 grouping and administration
The mice were randomly divided into 9 groups of 12 mice each, normal control group, model control group, 5 example groups (example 1 group, example 2 group, example 3 group, example 4 group and example 5 group), comparative example 1 group and comparative example 2 group, respectively. The test solutions of examples 1 to 5 obtained in 1.1 were used for intragastric administration in each example group, and the test solutions of comparative examples 1 and 2 obtained in 1.1 were used for intragastric administration in comparative example 1 and comparative example 2, respectively, and were continuously administered by intragastric administration for 5 days, once a day, with a volume of 0.2ml/10g body weight. The normal control group and the model control group were given an equivalent amount of 0.5% CMC-Na. 30min after the last administration, the animals are placed into a 10cm multiplied by 10cm acrylic box for cough induction, a WH-801 ultrasonic atomizer is adjusted to the 2 nd gear, ammonia water solution with the concentration of about 13% is sprayed into the box at a constant speed for 6s, a spraying pipe is removed, the mouth of the spraying pipe is exposed, and the cough latency and the cough frequency within 3min of the mice are recorded.
2.2 data analysis
The test data adopts SPSS18.0 statistical software, and the results are mean plus or minus standard deviation
Showing that one-way analysis of variance is adopted.
3. Results
3.1 Effect on Ammonia-induced cough in mice
Compared with the model control group, the cough latency of mice in the example group and the control group is obviously prolonged (P <0.05), the cough frequency is obviously reduced (P <0.05), and the cough latency of the example group is longer than that of the control group, and the cough frequency is less than that of the control group. See table 9.
TABLE 9 Effect on Ammonia-induced cough in mice
Note: comparison with model control group: p < 0.05.
4. Conclusion
The antibacterial and antiviral traditional Chinese medicine composition provided by the embodiment of the invention has an obvious inhibiting effect on mouse cough caused by ammonia water spray after in vivo administration, and is superior to a comparative example.
Effect example 5
The effect example investigates the bacteriostasis effect test of the antibacterial and antiviral traditional Chinese medicine composition on gram-positive bacteria and gram-negative bacteria so as to observe the bacteriostasis of the antibacterial and antiviral traditional Chinese medicine composition.
1. Material
1.1 test drugs
The raw materials and the preparation methods of examples 1 to 5 and comparative examples 1 and 2 were prepared by grinding the raw materials, dissolving the ground raw materials in dimethyl sulfoxide (DMSO) and performing ultrasonic assisted dissolution to obtain a solution with a concentration of 500mg crude drug/ml, and filtering the solution with a 0.22 μm microporous membrane to obtain a solution to be tested.
1.2 test strains
Gram-positive bacteria: staphylococcus aureus (ATCC6538), streptococcus pyogenes (ATCC 19615);
gram-negative bacteria: escherichia coli (ATCC35218) and Pseudomonas aeruginosa (ATCC 9027).
1.3 Medium
Beef extract peptone solid medium: 0.5g of beef extract, 0.5g of sodium chloride, 1.0g of peptone and 100mL of water, and the pH value is 7.0-7.2. Sterilizing at 121 deg.C under 0.1MPa for 20 min;
beef extract peptone liquid medium: 0.5g of beef extract, 0.5g of sodium chloride, 1.0g of peptone, 2.0-4.0 g of agar powder, 100mL of water and pH 7.0-7.2. Sterilizing at 121 deg.C under 0.1MPa for 20 min.
2. Method of producing a composite material
Washing thallus Porphyrae with sterile water from slant culture (24h) of 5 th generation nutrient agar culture medium of the test strain, and diluting with the above beef extract peptone liquid culture medium to obtain extract with concentration of 106cfu/ml bacterial suspension.
The solutions to be tested for each example and comparative example were operated as follows: and (3) taking the sterilized 96-well plate, and adding 100 mu L of beef extract peptone liquid culture medium into the 1 st to 11 th wells respectively. Adding 100 mu L of solution to be tested into the 1 st hole, sucking 100 mu L of the solution to be tested from the 1 st hole and adding the solution to the 2 nd hole after uniform mixing, sucking 100 mu L of the solution to be tested from the 2 nd hole and adding the solution to the 3 rd hole after uniform mixing, performing the operation till the 10 th hole, and finally sucking 100 mu L of the solution to be tested from the 10 th hole and discarding. 100 mu L of bacterial suspension is added into the 1 st to 12 th holes of a 96-well plate. To the 12 th well, 100. mu.L of dimethyl sulfoxide was added as a negative control. Each set was set to 3 parallel tests.
3. Results
The 96-well plate is placed in a constant-temperature incubator at 37 ℃ for 24h, and then is placed under a black background for observation, so that the minimum inhibitory concentration of each sample is obtained, and the results are shown in table 10.
TABLE 10 minimum inhibitory concentration (in crude drug)
4. Conclusion
The antibacterial and antiviral traditional Chinese medicine composition provided by the embodiment of the invention has an inhibiting effect on both gram-positive bacteria and gram-negative bacteria, and is superior to a comparative example.
Effect example 6
The effect example provides the clinical application effect of the antibacterial and antiviral traditional Chinese medicine composition of example 1.
1. Cases and treatment methods
The clinical application effect statistics is carried out on 102 patients who have the same symptoms and are attacked by wind and heat and have damp toxin and take the antibacterial and antiviral traditional Chinese medicine composition disclosed by the embodiment 1 of the invention in 6 months to 2020 and 7 months in 2018.
The average age of the patients is 34.4 years (the age range is 12-41 years), and the clinical symptoms are manifested by fever, itching, hidden rubella, mouth and tongue sores, swollen and sore throat, dry mouth, thirst, dry cough, shortness of breath, white phlegm, little blood streak, dry and hard stool, thin and yellow tongue coating, bright red tongue texture and floating and rapid pulse. Every person takes one dose of the antibacterial and antiviral traditional Chinese medicine composition provided by the embodiment 1 of the invention every day, no other medicine is used in the treatment period, the diet is light, cold food and cold drink below 4 ℃ are avoided, regular work and rest are avoided, cold air is prevented from blowing directly when the person is in an air-conditioning environment, and the temperature is not lower than 24 ℃.
2. Therapeutic effects
The clinical symptoms are completely disappeared as a cure standard.
According to statistics, the body temperature of the patients is reduced after the patients take the medicine for 1-2 days, and the rubella disappears and the swollen sore throat and dry stool condition is obviously relieved or disappeared after 2-4 days.
After treatment for 4-10 days, all the clinical symptoms disappear, and the cure rate is 100%.
The results show that the antibacterial and antiviral traditional Chinese medicine composition provided by the invention can effectively eliminate the above clinical symptoms of patients affected by wind heat and accompanied with damp toxin.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents or improvements made within the spirit and principle of the present invention should be included in the scope of the present invention.
Claims (10)
1. An antibacterial antiviral traditional Chinese medicine composition is characterized by comprising the following raw materials in parts by weight: 8-13 parts of honeysuckle, 7-11 parts of houttuynia cordata, 7-11 parts of wrinkled gianthyssop herb, 12-18 parts of gypsum, 4-8 parts of ephedra, 2-4 parts of menthol, 4-8 parts of rheum officinale, 7-11 parts of bitter almond and 2-4 parts of liquorice.
2. The antibacterial and antiviral traditional Chinese medicine composition as claimed in claim 1, which is characterized by comprising the following raw materials in parts by weight: 9-12 parts of honeysuckle, 8-10 parts of houttuynia cordata, 8-10 parts of wrinkled gianthyssop herb, 13-17 parts of gypsum, 5-7 parts of ephedra, 3-4 parts of menthol, 5-7 parts of rheum officinale, 8-10 parts of bitter almond and 3-4 parts of liquorice.
3. The antibacterial and antiviral traditional Chinese medicine composition as claimed in claim 2, which is characterized by comprising the following raw materials in parts by weight: 10 parts of honeysuckle, 9 parts of houttuynia cordata, 9 parts of agastache rugosus, 15 parts of gypsum, 6 parts of ephedra, 3 parts of menthol, 6 parts of rheum officinale, 9 parts of bitter almond and 3 parts of liquorice.
4. The method for preparing the antibacterial and antiviral traditional Chinese medicine composition as claimed in any one of claims 1 to 3, characterized by comprising the following operations: weighing the raw materials according to the raw material proportion in the antibacterial and antiviral traditional Chinese medicine composition, extracting volatile oil of the agastache rugosus, decocting residues with the honeysuckle, the houttuynia cordata, the gypsum, the ephedra, the rheum officinale and the bitter apricot seeds in water, filtering, collecting obtained filtrate, and drying to obtain a water-decocted extract; and adding the volatile oil of the agastache rugosus into the obtained water decoction extract, uniformly mixing, adding the menthol, and uniformly mixing to obtain the antibacterial antiviral traditional Chinese medicine composition.
5. The use of the antibacterial and antiviral Chinese medicinal composition of any one of claims 1 to 3 in the preparation of a medicament for relieving itching and eliminating rash.
6. The use of the antibacterial and antiviral traditional Chinese medicine composition of any one of claims 1 to 3 in the preparation of a medicament for treating aphthous stomatitis.
7. Use of the antibacterial and antiviral traditional Chinese medicine composition of any one of claims 1 to 3 in preparation of a medicament for treating sore throat.
8. Use of the antibacterial and antiviral traditional Chinese medicine composition as claimed in any one of claims 1 to 3 in the preparation of a medicament for treating dry cough.
9. Use of the antibacterial and antiviral traditional Chinese medicine composition of any one of claims 1 to 3 in the preparation of a medicament for treating at least two symptoms of rubella itching, aphthous stomatitis, sore throat, dry cough and dry stool.
10. An antiviral and/or antibacterial drug, which comprises the antibacterial and antiviral Chinese medicinal composition according to any one of claims 1 to 3.
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| CN1483463A (en) * | 2003-07-01 | 2004-03-24 | 河北以岭医药研究院有限公司 | Anti-virus Chinese medicine composition and preparation process thereof |
| CN102078574A (en) * | 2009-12-01 | 2011-06-01 | 唐超 | Traditional Chinese medicine pill for treating flu |
| CN105232911A (en) * | 2015-10-12 | 2016-01-13 | 菏泽高新区尊桂堂科技有限公司 | Traditional Chinese medicine composition |
| CN106074906A (en) * | 2016-07-27 | 2016-11-09 | 陈华平 | A kind of Chinese medicine composition treating flu |
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| CN1483463A (en) * | 2003-07-01 | 2004-03-24 | 河北以岭医药研究院有限公司 | Anti-virus Chinese medicine composition and preparation process thereof |
| CN102078574A (en) * | 2009-12-01 | 2011-06-01 | 唐超 | Traditional Chinese medicine pill for treating flu |
| CN105232911A (en) * | 2015-10-12 | 2016-01-13 | 菏泽高新区尊桂堂科技有限公司 | Traditional Chinese medicine composition |
| CN106074906A (en) * | 2016-07-27 | 2016-11-09 | 陈华平 | A kind of Chinese medicine composition treating flu |
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| WO2023226063A1 (en) * | 2022-05-27 | 2023-11-30 | 张鹏程 | Antiviral, antibacterial, and anti-inflammatory composition, dry powder, and application |
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