CN114081016B - Detection system for emergence rate of aphidius gifuensis and application method thereof - Google Patents
Detection system for emergence rate of aphidius gifuensis and application method thereof Download PDFInfo
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- CN114081016B CN114081016B CN202111404070.XA CN202111404070A CN114081016B CN 114081016 B CN114081016 B CN 114081016B CN 202111404070 A CN202111404070 A CN 202111404070A CN 114081016 B CN114081016 B CN 114081016B
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- 238000001514 detection method Methods 0.000 title claims abstract description 21
- 241001305034 Aphidius gifuensis Species 0.000 title claims description 22
- 238000000034 method Methods 0.000 title claims description 15
- 230000032669 eclosion Effects 0.000 claims abstract description 75
- 241000960374 Aphidiinae Species 0.000 claims abstract description 15
- 230000007246 mechanism Effects 0.000 claims abstract description 15
- 230000017448 oviposition Effects 0.000 claims abstract description 11
- 241001124076 Aphididae Species 0.000 claims description 36
- 238000007789 sealing Methods 0.000 claims description 20
- 235000002637 Nicotiana tabacum Nutrition 0.000 claims description 17
- 230000003071 parasitic effect Effects 0.000 claims description 15
- 244000061176 Nicotiana tabacum Species 0.000 claims description 12
- 238000009423 ventilation Methods 0.000 claims description 12
- 241000257303 Hymenoptera Species 0.000 claims description 7
- 241000721621 Myzus persicae Species 0.000 claims description 7
- 230000003203 everyday effect Effects 0.000 claims description 6
- 238000002474 experimental method Methods 0.000 claims description 6
- NIXOWILDQLNWCW-UHFFFAOYSA-N acrylic acid group Chemical group C(C=C)(=O)O NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 claims description 3
- 238000005485 electric heating Methods 0.000 claims description 3
- 229920003229 poly(methyl methacrylate) Polymers 0.000 claims description 3
- 239000004926 polymethyl methacrylate Substances 0.000 claims description 3
- 238000012360 testing method Methods 0.000 claims description 3
- 241000208125 Nicotiana Species 0.000 description 5
- 235000019504 cigarettes Nutrition 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 238000012986 modification Methods 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 238000007664 blowing Methods 0.000 description 2
- 239000007943 implant Substances 0.000 description 2
- 230000008676 import Effects 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 241000952610 Aphis glycines Species 0.000 description 1
- 241001600408 Aphis gossypii Species 0.000 description 1
- 241000982105 Brevicoryne brassicae Species 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 241000721623 Myzus Species 0.000 description 1
- 244000061458 Solanum melongena Species 0.000 description 1
- 235000002597 Solanum melongena Nutrition 0.000 description 1
- 241000209140 Triticum Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
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- 238000013461 design Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000035939 shock Effects 0.000 description 1
- 230000032258 transport Effects 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Life Sciences & Earth Sciences (AREA)
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- Animal Behavior & Ethology (AREA)
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- Biodiversity & Conservation Biology (AREA)
- Catching Or Destruction (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The application discloses a system for detecting the emergence rate of a rough ridge aphidiidae, which comprises a constant temperature cultivation room, an oviposition detection device, an emergence observation box and a storage box, wherein the constant temperature cultivation room is a cultivation greenhouse with one end open, the oviposition detection device and the emergence observation box are arranged in the constant temperature cultivation room, the oviposition detection device comprises a first base, a box body, a driving box and a driving mechanism, the lower part of the box body is provided with a supporting plate, the lower part of the supporting plate is connected with the first base through a telescopic rod, the upper part of the supporting plate is provided with a rectangular opening, the box body is arranged in the rectangular opening, the driving box is arranged in the lower part of the box body, and the driving mechanism is arranged in the driving box; the eclosion case is fixed to be set up in second base upper portion, and the eclosion case is upper portion open-ended rectangle cavity body structure, and eclosion case one end is provided with the discharge gate, and the discharge gate is connected with the containing box through the connecting pipe. The application can improve the eclosion efficiency by the common use of the spawning detection, eclosion observation box and constant temperature cultivation room.
Description
Technical Field
The application belongs to the technical field of biological control of agricultural pests, and particularly relates to a system for detecting the emergence rate of aphidius gifuensis and a use method thereof.
Background
The aphidius gifuensis is one of natural enemy insects with high utilization value in the aphidiidae by virtue of excellent properties such as strong fertility, high natural parasitic rate, short generation period, strong adaptability, easiness in artificial propagation and the like. The bees are mainly distributed in China (including Taiwan area of China), korea, japan and other Asian eastern areas, have strong natural control ability on host aphids, have wide host range, can parasitize myzus persicae, aphis Raphani, wheat long-tube aphid, soybean aphid, cotton aphid, eggplant net aphid and the like, and have particularly remarkable control effect on the myzus persicae. In tobacco fields, the parasitic rate of the aphidius gifuensis to the myzus gifuensis is usually 20% -60%, and the parasitic rate can reach 89.16%. In the cultivation process of the rough ridge aphidiidae, the detection of spawning and eclosion is an important step in the whole cultivation process, multiple experimental processes are required to be recorded, and optimal indoor conditions of spawning and eclosion are obtained by comparing data, so that a detection system capable of rapidly and accurately obtaining the eclosion rate of the rough ridge aphidiidae is urgently needed.
Disclosure of Invention
The application aims to provide a system for detecting the emergence rate of a rough ridge aphidiidae and a use method thereof, which can improve the emergence efficiency through the common use of an egg laying detection, emergence observation box and a constant temperature cultivation room.
In order to solve the technical problems, the application adopts the following technical scheme:
the system comprises a constant temperature cultivation room, an oviposition detection device, an eclosion observation box and a storage box, wherein the constant temperature cultivation room is a cultivation greenhouse with one end open, the oviposition detection device and the eclosion observation box are arranged in the constant temperature cultivation room, the oviposition detection device comprises a first base, a box body, a driving box and a driving mechanism, a supporting plate is arranged at the lower part of the box body, the lower part of the supporting plate is connected with the first base through a telescopic rod, a rectangular opening is formed in the upper part of the supporting plate, the box body is arranged in the rectangular opening, the driving box is arranged at the lower part of the box body, and the driving mechanism is arranged in the driving box; the eclosion box is fixedly arranged on the upper part of the second base, is of a rectangular cavity structure with an opening at the upper part, and is provided with a discharge hole at one end, and the discharge hole is connected with the storage box through a connecting pipe; an air guide device is arranged in the eclosion box.
Further, the driving mechanism comprises a driving motor, a bearing seat, a rotating shaft, a synchronizing wheel, a carrying disc and a synchronizing belt, wherein the rotating shaft and the synchronizing wheel connected with the rotating shaft are arranged at the upper part of the bearing seat, the synchronizing wheel is connected with the other synchronizing wheel on the driving motor through the synchronizing belt, and the carrying disc is arranged at the upper part of the rotating shaft; the outer wall and the upper part of the box body are respectively provided with a ventilation plate and a sealing cover.
Further, sliding strips are arranged on two side edges of the supporting plate and are in sliding connection with sliding grooves on the first base; an observation port is formed in the outer wall of the box body, and an acrylic transparent plate is arranged on the observation port; the outer wall of the telescopic rod is provided with a pressure spring; the upper part of the ventilation plate and the upper part of the sealing cover are respectively provided with ventilation holes.
Further, the upper part of the first base is provided with force arms, the middle parts of the two force arms are provided with push rods, the upper part of each push rod is provided with a flexible rubber sleeve, and the outer wall of each flexible rubber sleeve is provided with an anti-skid stripe; the lower part of the first base is provided with a plurality of universal wheels, and the universal wheels are at least provided with four.
Further, the air guide device comprises a first slide bar, an air guide push plate, an air guide pipe, an air guide box and a pneumatic cylinder, wherein two ends of the air guide push plate are in sliding connection with the first slide bar on the inner wall of the eclosion box through sliding grooves, and one end of the air guide push plate is connected with the air guide box through the air guide pipe; one end of the eclosion box is provided with a pneumatic cylinder which is connected with the air guide push plate through a driving rod; one end of the eclosion box is provided with a sealing plate.
Further, the air guide pushing plate is of a cavity structure, and one end of the air guide pushing plate is provided with a plurality of air guide holes; the air guide box is arranged at the air inlet of the eclosion box, and an electric heating pipe and an air guide fan are arranged in the eclosion box; the upper part of the eclosion box is provided with a sealing cover, and the upper part of the sealing cover is provided with a plurality of ventilation holes; both side sections of the eclosion box are provided with acrylic glass plates; the containing box is of a rectangular cavity structure with one end being open, and a collecting box is arranged inside the containing box and is in sliding connection with a second sliding groove in the containing box through a second sliding strip at the bottom.
Further, a feeding hole matched with the connecting pipe is formed in one end of the collecting box, and a handle is arranged at one end of the collecting box; the side part of the eclosion box is provided with a feeding pipe, and the upper part of the feeding pipe is provided with a feeding hopper; the bottom of the second base is provided with a plurality of supporting blocks, and the supporting blocks are provided with protection gaskets; the pneumatic cylinder is fixedly arranged at one end of the eclosion box through the supporting seat.
Further, the raw aphidius gifuensis that collects in the collecting box is transferred to the storage box.
The application method of the system for detecting the emergence rate of the aphidius gifuensis comprises the following steps:
s1, taking a tobacco plant 203 of Yunyan, placing the tobacco plant 203 on a carrying tray of a box body, placing tobacco aphids on the tobacco plant to naturally reproduce the tobacco plant, covering a sealing cover for carrying out, and placing the whole equipment into a constant-temperature cultivation room for standby;
s2, observing the number of the aphids in the box body, driving the whole carrying disc through a lower driving mechanism, observing under the rotation of the carrying disc, when the number of the aphids reaches 2000, placing the female aphids of the Aphidius gifuensis for pairing, and counting the number of parasitic aphids every day;
s3, repeating the whole experiment of S2 for 20 times, and recording the quantity of parasitic aphids each time, wherein the spawning quantity head=the total quantity head of the parasitic aphids;
s4, inoculating the parasitic aphids obtained in the step S3 to bees through a ratio of 1:30, collecting stiff aphid leaves when the stiff aphids are formed in a large quantity, randomly picking the stiff aphids by a soft hair pen, carrying out eclosion in an eclosion box, counting the eclosion number of adult bees at regular time every day, and ending the test when the eclosion is not carried out for five continuous days;
s5, driving an air guide push plate in the eclosion box, and guiding the inside rough ridge aphidiidae into a collecting box of the containing box to finish collection.
In the step S1, the temperature in the constant temperature cultivating chamber is 20-30 ℃ and the humidity is 70-75%; in the step S4, the temperature of the eclosion box is 24-26 ℃ and the humidity is 90-95%.
Compared with the prior art, the application has at least one of the following beneficial effects:
1. the whole system adopts constant temperature culture room, spawning detection device, eclosion observation box and bin part to constitute, wherein, spawning detection device is provided with box and driving box on first base upper portion, places cloud cigarette 203 tobacco strain on the carrying tray of driving box inside to settle the cigarette aphid simultaneously, be convenient for the cigarette aphid to grow on the tobacco strain, and implant 1 in the box thick ridge aphidiidae female bee, be convenient for parasitize, and whole carrying tray can rotate through the driving motor of lower part, be convenient for operating personnel observe in the outside, improve the practicality of equipment.
2. The eclosion observation box that adopts is provided with the eclosion case on second base upper portion, can be used for placing thick ridge aphidiidae, is convenient for carry out the eclosion in inside, and the inside of eclosion case is settled there is independent air guide device, wherein, air guide push pedal passes through spout and first draw runner sliding connection of both sides to drive through the pneumatic cylinder of lateral part, realize advancing the processing, and air guide push pedal is inside to be cavity body structure, can cooperate the air duct to realize the import of air current, is collecting through blowing and air guide push pedal's impelling down thick ridge aphidiidae to realize collecting in the containing box.
3. In the whole system, the spawning detection device and the eclosion observation box are all carried out in a constant-temperature cultivation room, experiments are carried out under the condition of controlling the temperature and the humidity, and the optimal inoculation method is obtained after multiple experiments.
Drawings
FIG. 1 is a schematic diagram of the structure of the present application.
FIG. 2 is a front view of the spawning detection device of the present application.
Fig. 3 is a schematic view of a first base portion of the present application.
Fig. 4 is a schematic view of the internal structure of the driving box of the present application.
Fig. 5 is a schematic structural view of the present application.
FIG. 6 is a front view of the inventive feathering observation box.
FIG. 7 is a top view of the feathering box of the present application.
FIG. 8 is a schematic view of a portion of an air guide device according to the present application.
In the figure: 1-first base, 2-chute, 3-telescopic link, 4-compression spring, 5-backup pad, 6-box, 7-aeration board, 8-sealed lid, 10-arm of force, 11-push rod, 12-flexible rubber sleeve, 13-viewing port, 14-driving case, 15-universal wheel, 17-bearing frame, 18-synchronizing wheel, 19-pivot, 20-carrying tray, 21-hold-in range, 22-driving motor, 23-sealing plate, 31-second base, 32-eclosion case, 33-inlet pipe, 34-feeder hopper, 35-sealed lid, 36-bleeder vent, 37-pneumatic cylinder, 38-air guide fan, 39-air guide case, 40-containing box, 41-collecting box, 42-handle, 44-connecting tube, 46-first slide bar, 47-air guide push plate, 49-air guide tube, 51-supporting seat.
Detailed Description
In order to make the objects, technical solutions and advantages of the present application more apparent, the following description is taken in conjunction with the accompanying drawings
And examples, the present application will be described in further detail. It should be understood that the specific embodiments described herein
The examples are only for explaining the present application and are not intended to limit the present application.
In the description of the present application, it should be understood that "front, rear, upper, lower, left, right", "inner
The orientation or positional relationship indicated by the outer "," bottom "," top ", etc. is generally based on the orientation or position shown in the drawings
Relationships, for convenience of description and simplification of the description, are provided for the purpose of not contrary description of the present application
The directional terms do not indicate or imply that the device or element in question must have a particular orientation or be in a particular orientation
Bit configuration and operation, and therefore should not be construed as limiting the scope of the application.
Example 1
Referring to fig. 1 to 8, a system for detecting emergence rate of rough ridge aphidiidae comprises a constant temperature cultivation room, a spawning detection device, an emergence observation box and a storage box, and is characterized in that: the constant temperature cultivating room is a cultivating greenhouse with one end being opened, an egg laying detecting device and a eclosion observing box are arranged in the constant temperature cultivating room, the egg laying detecting device comprises a first base 1, a box body 6, a driving box 14 and a driving mechanism, a supporting plate 5 is arranged at the lower part of the box body 6, the lower part of the supporting plate 5 is connected with the first base 1 through a telescopic rod 3, a rectangular opening is formed in the upper part of the supporting plate 5, the box body 6 is arranged in the rectangular opening, the driving box 14 is arranged at the lower part of the box body 6, the driving mechanism eclosion box 32 is fixedly arranged at the upper part of a second base 31, the eclosion box 32 is of a rectangular cavity structure with an opening at the upper part, a discharge hole is formed in one end of the eclosion box 32, and the discharge hole is connected with a storage box 40 through a connecting pipe 44; an air guide device is arranged inside the eclosion box 32; in the above-mentioned scheme, whole detecting system adopts constant temperature culture room, spawning detection device, eclosion observation box and bin part to constitute, realize detecting through three equipment combined action down, wherein, spawning detection device is provided with box and driving box on first base upper portion, place cloud cigarette 203 tobacco strain on the thing dish that carries of driving box inside, and settle the tobacco aphid simultaneously, the tobacco aphid of being convenient for grows on the tobacco strain, and implant thick spine aphid cocoon bee female bee 1 in the box, be convenient for parasitize, and whole carrying dish can rotate through the driving motor of lower part, the operating personnel of being convenient for observe in the outside, the practicality of improvement equipment.
Example 2:
the embodiment further describes the supporting plate 5 on the basis of the existing embodiment 1, the driving mechanism comprises a driving motor 22, a bearing seat 17, a rotating shaft 19, a synchronizing wheel 18, a carrying disc 20 and a synchronizing belt 21, the rotating shaft 19 and the synchronizing wheel 18 connected with the rotating shaft 19 are arranged at the upper part of the bearing seat 17, the synchronizing wheel 18 is connected with another synchronizing wheel on the driving motor 22 through the synchronizing belt 21, and the carrying disc 20 is arranged at the upper part of the rotating shaft 19; the outer wall and the upper part of the box body 6 are respectively provided with a ventilation plate 7 and a sealing cover 8; the driving mechanism at the bottom of the carrying tray head drives the carrying tray head to rotate, so that the carrying tray head is convenient for operators to observe, and the practicability of the equipment is improved.
Example 3:
the embodiment further describes the supporting plate 5 based on the existing embodiment 1, wherein sliding strips are arranged on two side edges of the supporting plate 5 and are in sliding connection with the sliding grooves 2 on the first base 1; an observation port 13 is arranged on the outer wall of the box body 6, and an acrylic transparent plate is arranged on the observation port 13; the outer wall of the telescopic rod 3 is provided with a pressure spring 4; the ventilation holes are formed in the upper portions of the ventilation plate 7 and the sealing cover 8, and the telescopic rod 3 and the pressure spring can play a role in shock absorption in cooperation with operators in the transferring process, so that the practicability of the equipment is improved.
Example 4:
the embodiment further describes the first base 1 on the basis of the existing embodiment 1, wherein the upper part of the first base 1 is provided with force arms 10, the middle parts of the two force arms 10 are provided with push rods 11, the upper parts of the push rods 11 are provided with flexible rubber sleeves 12, and the outer walls of the flexible rubber sleeves 12 are provided with anti-skid stripes; the lower part of the first base 1 is provided with a plurality of universal wheels 15, and at least four universal wheels 15 are arranged; the design of push rod 11 can be convenient for operating personnel carry out the pushing, transports whole equipment to other places.
Example 5:
the embodiment is further described on the basis of the existing embodiment 1, the air guide device includes a first slide bar 46, an air guide pushing plate 47, an air guide pipe 49, an air guide box 39 and a pneumatic cylinder 37, two ends of the air guide pushing plate 47 are slidably connected with the first slide bar 46 on the inner wall of the feathering box 32 through a chute, and one end of the air guide pushing plate 47 is connected with the air guide box 39 through the air guide pipe 49; one end of the eclosion box 32 is provided with a pneumatic cylinder 37, and the pneumatic cylinder 37 is connected with an air guide push plate 47 through a driving rod; one end of the feathering box 32 is provided with a sealing plate 23; the air guide pushing plate 47 is of a cavity structure, and one end of the air guide pushing plate 47 is provided with a plurality of air guide holes; the air guide box 39 is arranged at the air inlet of the eclosion box 32, and an electric heating pipe and an air guide fan 38 are arranged inside the eclosion box 32; the upper part of the eclosion box 32 is provided with a sealing cover 35, and the upper part of the sealing cover 35 is provided with a plurality of ventilation holes 36; both side sections of the feathering box 32 are provided with acrylic glass plates; the storage box 40 is of a rectangular cavity structure with one end open, a collection box 41 is arranged in the storage box 40, and the collection box 41 is in sliding connection with a second sliding groove in the storage box 40 through a second sliding strip at the bottom; the eclosion observation box that adopts is provided with the eclosion case on second base upper portion, can be used for placing thick ridge aphidiidae, is convenient for carry out the eclosion in inside, and the inside of eclosion case is settled there is independent air guide device, wherein, air guide push pedal passes through spout and first draw runner sliding connection of both sides to drive through the pneumatic cylinder of lateral part, realize advancing the processing, and air guide push pedal is inside to be cavity body structure, can cooperate the air duct to realize the import of air current, is collecting through blowing and air guide push pedal's impelling down thick ridge aphidiidae to realize collecting in the containing box.
Example 6:
in this embodiment, the air guide device is further described based on the existing embodiment 1, a feeding hole matched with a connecting pipe 44 is provided at one end of the collecting box 41, and a handle 42 is provided at one end of the collecting box 41; the side of the eclosion box 32 is provided with a feed pipe 33, and the upper part of the feed pipe 33 is provided with a feed hopper 34; a plurality of supporting blocks are arranged at the bottom of the second base 31, and a protection gasket is arranged on each supporting block; the pneumatic cylinder 37 is fixedly arranged at one end of the eclosion box 32 through the supporting seat 51; the raw aphidius gifuensis that is collected in the collection box 41 is transferred to the storage box; the feed hopper 34 can facilitate an operator to throw materials into the feed hopper from the outside without repeatedly opening an upper sealing cover, so that the feed is facilitated; the collecting box 41 is movably connected with the collecting box, a circular opening is formed in the collecting box, and after the collecting box is pushed into the collecting box, a circular hole of the collecting box can correspond to the connecting pipe, so that direct feeding is facilitated, and rapid collection can be achieved.
Example 7:
the application method of the system for detecting the emergence rate of the aphidius gifuensis comprises the following steps:
s1, taking a tobacco plant 203 of Yunyan, placing the tobacco plant 203 on a carrying tray of a box body, placing tobacco aphids on the tobacco plant to naturally reproduce the tobacco plant, covering a sealing cover for carrying out, and placing the whole equipment into a constant-temperature cultivation room for standby;
s2, observing the number of the aphids in the box body, driving the whole carrying disc through a lower driving mechanism, observing under the rotation of the carrying disc, when the number of the aphids reaches 2000, placing the female aphids of the Aphidius gifuensis for pairing, and counting the number of parasitic aphids every day;
s3, repeating the whole experiment of S2 for 20 times, and recording the quantity of parasitic aphids each time, wherein the spawning quantity head=the total quantity head of the parasitic aphids;
s4, inoculating the parasitic aphids obtained in the step S3 to bees through a ratio of 1:30, collecting stiff aphid leaves when the stiff aphids are formed in a large quantity, randomly picking the stiff aphids by a soft hair pen, carrying out eclosion in an eclosion box, counting the eclosion number of adult bees at regular time every day, and ending the test when the eclosion is not carried out for five continuous days;
s5, driving an air guide push plate in the eclosion box, and guiding the inside rough ridge aphidiidae into a collecting box of the containing box to finish collection.
In the step S1, the temperature in the constant temperature cultivating chamber is 25 ℃ and the humidity is 72.5%; the temperature of the feathering box in the S4 step was 25℃and the humidity was 92%.
And carrying out multiple experiments by matching with the spawning detection device and the eclosion observation box in the system, and collecting data to achieve the optimal cultivation environment.
The appearances of the phrase "in various places in the specification are not necessarily all referring to the same embodiment. Further, in the further aspect, the method comprises the steps of,
when a particular feature, structure, or characteristic is described in connection with any embodiment, it is submitted that it is in connection with other implementations
Embodiments to achieve such features, structures or characteristics are also within the scope of the present application.
Although the application has been described herein with reference to a number of illustrative embodiments thereof, it should be understood that numerous other modifications and embodiments can be devised by those skilled in the art that will fall within the scope and spirit of the principles of this disclosure. More specifically, various variations and modifications may be made to the component parts and/or arrangements of the subject combination arrangement within the scope of the disclosure, drawings and claims. In addition to variations and modifications in the component parts and/or arrangements, other uses will be apparent to those skilled in the art.
Claims (10)
1. The utility model provides a thick ridge aphidiidae emergence rate detecting system, includes constant temperature culture room, spawning detection device, emergence observation box and bin, its characterized in that: the constant temperature cultivating room is a cultivating greenhouse with one end being opened, an egg laying detecting device and a eclosion observing box are arranged in the constant temperature cultivating room, the egg laying detecting device comprises a first base (1), a box body (6), a driving box (14) and a driving mechanism, a supporting plate (5) is arranged at the lower part of the box body (6), the lower part of the supporting plate (5) is connected with the first base (1) through a telescopic rod (3), a rectangular opening is formed in the upper part of the supporting plate (5), the box body (6) is arranged in the rectangular opening, the driving box (14) is arranged at the lower part of the box body (6), and the driving mechanism is arranged in the driving box (14);
the eclosion box (32) is fixedly arranged on the upper part of the second base (31), the eclosion box (32) is of a rectangular cavity structure with an opening at the upper part, one end of the eclosion box (32) is provided with a discharge port, and the discharge port is connected with the storage box (40) through a connecting pipe (44); an air guide device is arranged in the eclosion box (32).
2. The system for detecting the emergence rate of the aphidius gifuensis according to claim 1, wherein the system is characterized in that: the driving mechanism comprises a driving motor (22), a bearing seat (17), a rotating shaft (19), a synchronizing wheel (18), a carrying disc (20) and a synchronizing belt (21), wherein the rotating shaft (19) and the synchronizing wheel (18) connected with the rotating shaft (19) are arranged at the upper part of the bearing seat (17), the synchronizing wheel (18) is connected with the other synchronizing wheel on the driving motor (22) through the synchronizing belt (21), and the carrying disc (20) is arranged at the upper part of the rotating shaft (19); the outer wall and the upper part of the box body (6) are respectively provided with a ventilation plate (7) and a sealing cover (8).
3. The system for detecting the emergence rate of the aphidius gifuensis according to claim 1, wherein the system is characterized in that: sliding strips are arranged on two side edges of the supporting plate (5), and are in sliding connection with sliding grooves (2) on the first base (1); an observation port (13) is arranged on the outer wall of the box body (6), and an acrylic transparent plate is arranged on the observation port (13); the outer wall of the telescopic rod (3) is provided with a pressure spring (4); the upper parts of the ventilation plates (7) and the sealing covers (8) are provided with ventilation holes.
4. The system for detecting the emergence rate of the aphidius gifuensis according to claim 1, wherein the system is characterized in that: the anti-slip device is characterized in that the upper part of the first base (1) is provided with force arms (10), the middle parts of the two force arms (10) are provided with push rods (11), the upper part of each push rod (11) is provided with a flexible rubber sleeve (12), and the outer wall of each flexible rubber sleeve (12) is provided with anti-slip stripes; the lower part of the first base (1) is provided with a plurality of universal wheels (15), and the universal wheels (15) are at least provided with four.
5. The system for detecting the emergence rate of the aphidius gifuensis according to claim 1, wherein the system is characterized in that: the air guide device comprises a first sliding strip (46), an air guide push plate (47), an air guide pipe (49), an air guide box (39) and an air cylinder (37), wherein two ends of the air guide push plate (47) are in sliding connection with the first sliding strip (46) on the inner wall of the eclosion box (32) through sliding grooves, and one end of the air guide push plate (47) is connected with the air guide box (39) through the air guide pipe (49); one end of the eclosion box (32) is provided with a pneumatic cylinder (37), and the pneumatic cylinder (37) is connected with an air guide push plate (47) through a driving rod; one end of the eclosion box (32) is provided with a sealing plate (23).
6. The system for detecting the emergence rate of the aphidius gifuensis according to claim 5, wherein the system is characterized by: the air guide pushing plate (47) is of a cavity structure, and one end of the air guide pushing plate (47) is provided with a plurality of air guide holes; the air guide box (39) is arranged at the air inlet of the eclosion box (32), and an electric heating pipe and an air guide fan (38) are arranged in the eclosion box (32); the upper part of the eclosion box (32) is provided with a sealing cover (35), and the upper part of the sealing cover (35) is provided with a plurality of ventilation holes (36); both side sections of the eclosion box (32) are provided with acrylic glass plates; the containing box (40) is of a rectangular cavity structure with one end open, a collecting box (41) is arranged inside the containing box (40), and the collecting box (41) is in sliding connection with a second sliding groove in the containing box (40) through a second sliding strip at the bottom.
7. The system for detecting the emergence rate of the aphidius gifuensis according to claim 6, wherein the system is characterized in that: one end of the collecting box (41) is provided with a feeding hole matched with the connecting pipe (44), and one end of the collecting box (41) is provided with a handle (42); a feed pipe (33) is arranged at the side part of the eclosion box (32), and a feed hopper (34) is arranged at the upper part of the feed pipe (33); a plurality of supporting blocks are arranged at the bottom of the second base (31), and protection gaskets are arranged on the supporting blocks; the pneumatic cylinder (37) is fixedly arranged at one end of the eclosion box (32) through the supporting seat (51).
8. The system for detecting the emergence rate of the aphidius gifuensis according to claim 7, wherein the system is characterized in that: the raw aphidius gifuensis that is collected in the collection box (41) is transferred to the storage box.
9. The use method of the system for detecting the emergence rate of the aphidius gifuensis according to claim 1, comprising the following steps:
s1, taking a tobacco plant 203 of Yunyan, placing the tobacco plant 203 on a carrying tray of a box body, placing tobacco aphids on the tobacco plant to naturally reproduce the tobacco plant, covering a sealing cover for carrying out, and placing the whole equipment into a constant-temperature cultivation room for standby;
s2, observing the number of the aphids in the box body, driving the whole carrying disc through a lower driving mechanism, observing under the rotation of the carrying disc, when the number of the aphids reaches 2000, placing the female aphids of the Aphidius gifuensis for pairing, and counting the number of parasitic aphids every day;
s3, repeating the whole experiment of S2 for 20 times, and recording the number of parasitic aphids each time, wherein the spawning amount (head) =the total amount (head) of the parasitic aphids;
s4, inoculating the parasitic aphids obtained in the step S3 to bees through a ratio of 1:30, collecting stiff aphid leaves when the stiff aphids are formed in a large quantity, randomly picking the stiff aphids by a soft hair pen, carrying out eclosion in an eclosion box, counting the eclosion number of adult bees at regular time every day, and ending the test when the eclosion is not carried out for five continuous days;
s5, driving an air guide push plate in the eclosion box, and guiding the inside rough ridge aphidiidae into a collecting box of the containing box to finish collection.
10. The method for using the system for detecting the emergence rate of the aphidius gifuensis according to claim 9, wherein the method comprises the following steps: in the step S1, the temperature in the constant temperature cultivating chamber is 20-30 ℃ and the humidity is 70-75%; in the step S4, the temperature of the eclosion box is 24-26 ℃ and the humidity is 90-95%.
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