CN114062101A - Sample processing method and device - Google Patents
Sample processing method and device Download PDFInfo
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- CN114062101A CN114062101A CN202010750703.1A CN202010750703A CN114062101A CN 114062101 A CN114062101 A CN 114062101A CN 202010750703 A CN202010750703 A CN 202010750703A CN 114062101 A CN114062101 A CN 114062101A
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- sample
- enrichment
- concentrator
- sample container
- container
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
Abstract
The application discloses a sample processing method, which comprises the following steps: enriching a sample in a sample container using an enricher in combination with the sample container; allowing the concentrator to provide an enriched sample for detection; placing the concentrator into the sample container and discarding therewith. The application also provides a device using the sample processing method. The application provides a sample processing method and a sample processing device, wherein the two disposable articles, namely an enriching device and a sample container, are discarded together without respectively discarding the two disposable articles, so that the processing difficulty of detection waste is reduced; meanwhile, the enriching device does not need to be provided with a separate discarding outlet, and only needs to be placed into a sample container like the operation of enriching a sample, so that the whole detection instrument only needs one waste discarding path and outlet, and the structure and the operation logic of the instrument can be simplified.
Description
Technical Field
The present application relates to the field of medical testing technologies, and in particular, to a method and an apparatus for processing a sample.
Background
The medical examination is to examine a sample such as collected blood, body fluid, secretion, excrement and cast by visual observation, microscopy, physical, chemical, instrumental or molecular biological methods.
When the sample is a solid substance or contains a solid substance, the existing detection method is to add a diluent to dilute the sample to form a suspension, and then take part of the suspension to sample for detection. However, the sampling amount is small, which may cause missed detection and affect the accuracy of the detection result.
In order to increase the detectable rate of the visible components in the sample, the sample needs to be enriched. The existing enrichment methods mainly comprise a centrifugal precipitation method, a natural precipitation method and the like. The centrifugal precipitation method is to remove supernatant after a sample is centrifuged, take precipitate for detection, but send the diluted sample into a centrifuge for centrifugation, and is complex to operate; the natural precipitation method is to remove supernatant after a sample is stood for a certain time to form fractional precipitation and take precipitate for detection, but the natural precipitation needs longer waiting time and has lower detection efficiency.
Therefore, how to improve the detection efficiency on the premise of ensuring the detection accuracy is a technical problem to be urgently solved by the technical personnel in the field.
Disclosure of Invention
To solve the above technical problems, a first object of the present invention is to provide a sample processing method; a second object of the present invention is to provide an apparatus for processing a sample using the above sample processing method; the application provides a sample processing method and a sample processing device, wherein the two disposable articles, namely an enriching device and a sample container, are discarded together without respectively discarding the two disposable articles, so that the processing difficulty of detection waste is reduced; meanwhile, the enriching device does not need to be provided with a separate discarding outlet, and only needs to be placed into a sample container like the operation of enriching a sample, so that the whole detection instrument only needs one waste discarding path and outlet, and the structure and the operation logic of the instrument can be simplified.
The technical scheme provided by the invention is as follows:
a method of sample processing comprising the steps of:
enriching a sample in a sample container using an enricher in combination with the sample container;
allowing the concentrator to provide an enriched sample for detection;
discarding the concentrator along with the sample container.
Preferably, the step of discarding the concentrator together with the sample container is embodied as: the spotted concentrator is combined with or contained by the sample container, and then moved with the sample container to be discarded.
Preferably, the method further comprises the step of diluting said sample prior to enrichment.
Preferably, the method further comprises the step of mixing the sample after dilution.
Preferably, the concentrator comprises an enrichment cavity surrounded by an enrichment shell, a barrier member and a suction connecting part, wherein the barrier member is respectively connected with the enrichment shell;
the enrichment step specifically comprises the following steps:
connecting the suction connection to an enrichment power unit and immersing the barrier in the sample;
and operating the enrichment power device to provide negative pressure, so that the sample is sucked into the enrichment cavity, and meanwhile, the tangible matters in the sample are retained on the barrier to form a retentate.
Preferably, the method further comprises the step of blowing air before suction: operating the enrichment power plant to provide positive pressure to allow gas to enter the sample container.
Preferably, the enriching device provides the enriched sample for detection specifically as follows: the enricher is separated from the sample container and moved to spot the enriched sample onto a carrier for detection.
Preferably, the enrichment power device moves the spotted concentrator to above the sample container, then disengages the concentrator from the enrichment power device, combines the spotted concentrator with the sample container or is accommodated by the sample container, and then the concentrator moves together with the sample container and is discarded.
Preferably, the printing is in particular press printing.
An apparatus for processing a sample using the sample processing method of any one of the above, comprising: an enrichment power device, a sample conveying device and a workpiece conveying device,
the enrichment power device is used for being connected with the enricher, providing sample enrichment power and driving the enricher to move;
the sample transport device is used for transporting sample containers;
the workpiece conveying device is used for conveying the enricher.
Aiming at the problems of complex operation or long time consumption of the existing enrichment method, the application provides a sample processing method, wherein an enrichment device is used for enriching a sample immediately when the sample is extracted, then the enriched sample is used for detection, and the enrichment device after sample application and a sample container are discarded together without respectively discarding the two disposable articles, so that the processing difficulty of detection waste is reduced; meanwhile, the enrichment device does not need to be provided with a separate discarding outlet, and only needs to be placed together with the sample container like the operation of enriching the sample, so that the whole detection instrument only needs one path and an outlet for discarding the waste, and the structure and the operation logic of the instrument can be simplified.
Drawings
In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings needed to be used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments described in the present application, and other drawings can be obtained by those skilled in the art without creative efforts.
FIG. 1 is a schematic diagram of a sample processing device disposed in a testing instrument according to an embodiment of the present invention;
FIG. 2 is a schematic diagram of a portion of a sample processing device disposed in a testing instrument according to an embodiment of the present invention;
FIG. 3 is a schematic diagram of a portion of a sample processing device disposed in a testing instrument according to an embodiment of the present invention;
reference numerals: 1-an enrichment power plant; 2-a sample transport device; and 3, a workpiece conveying device.
Detailed Description
In order to make those skilled in the art better understand the technical solutions in the present application, the technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are only a part of the embodiments of the present application, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.
It will be understood that when an element is referred to as being "fixed" or "disposed" on another element, it can be directly on the other element or be indirectly disposed on the other element; when an element is referred to as being "connected to" another element, it can be directly connected to the other element or be indirectly connected to the other element.
It will be understood that the terms "length," "width," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like, refer to an orientation or positional relationship illustrated in the drawings for convenience in describing the present application and to simplify description, and do not indicate or imply that the referenced device or element must have a particular orientation, be constructed and operated in a particular orientation, and thus should not be construed as limiting the present application.
Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present application, the meaning of a plurality of or a plurality of is two or more unless specifically limited otherwise.
It should be understood that the structures, ratios, sizes, and the like shown in the drawings are only used for matching the disclosure of the specification, so as to be understood and read by those skilled in the art, and are not used to limit the practical limit conditions of the present application, so that the modifications of the structures, the changes of the ratio relationships, or the adjustment of the sizes, do not have the technical essence, and the modifications, the changes of the ratio relationships, or the adjustment of the sizes, are all within the scope of the technical contents disclosed in the present application without affecting the efficacy and the achievable purpose of the present application.
The embodiments of the present application are written in a progressive manner.
As shown in fig. 1 to 3, an embodiment of the invention provides a sample processing method, including the following steps:
enriching a sample in a sample container using an enricher in combination with the sample container;
allowing the concentrator to provide an enriched sample for detection;
discarding the concentrator along with the sample container.
Aiming at the problems of complex operation or long time consumption of the existing enrichment method, the application provides a sample processing method, wherein an enrichment device is used for enriching a sample immediately when the sample is extracted, then the enriched sample is used for detection, and the enrichment device after sample application and a sample container are discarded together without respectively discarding the two disposable articles, so that the processing difficulty of detection waste is reduced; meanwhile, the enrichment device does not need to be provided with a separate discarding outlet, and only needs to be placed together with the sample container like the operation of enriching the sample, so that the whole detection instrument only needs one path and an outlet for discarding the waste, and the structure and the operation logic of the instrument can be simplified.
Preferably, the step of discarding the concentrator together with the sample container is embodied as: the spotted concentrator is combined with or contained by the sample container, and then moved with the sample container to be discarded.
Specifically, when the sample container is large enough to hold the concentrator, the concentrator can be dropped into the sample container, held by the sample container, and then discarded together; when the sample container itself is small or the opening of the sample container is small, the concentrator is placed at the opening of the sample container, or the concentrator is combined with the sample container and then both are discarded together.
Preferably, the method further comprises the step of diluting said sample prior to enrichment.
Preferably, the method further comprises the step of mixing the sample after dilution.
The sample processing method provided by the application preferably further comprises a dilution step, wherein the sample is diluted by using a dilution liquid, so that the tangible matters in the sample can be separated and enriched on the enriching device. More preferably, dilution is followed by homogenisation to ensure that the sample is evenly dispersed.
The dilution and homogenization steps are accomplished using parts commonly used in the art (e.g., sample needles, stir bars, etc.). Diluting and uniformly mixing can be carried out at the same station, and then diluent is injected firstly and then stirring is carried out at the same position; the liquid injection and the stirring can be completed by the same part, and different parts can be combined. Dilution and mixing can also be accomplished at different stations, and then the sample after diluting is moved another station and is carried out the mixing operation, dilutes the station simultaneously and can dilute next sample, improves the efficiency of handling the sample.
Preferably, the concentrator comprises an enrichment cavity surrounded by an enrichment shell, a barrier member and a suction connecting part, wherein the barrier member is respectively connected with the enrichment shell;
the enrichment step specifically comprises the following steps:
connecting the suction connection to an enrichment power unit and immersing the barrier in the sample;
and operating the enrichment power device to provide negative pressure, so that the sample is sucked into the enrichment cavity, and meanwhile, the tangible matters in the sample are retained on the barrier to form a retentate.
The enrichment device used in the application is preferably of a structure comprising an enrichment cavity, a blocking piece and a suction connecting part, when the enrichment device is used, the blocking piece is immersed in a sample of a sample container, the enrichment power device is connected with the suction connecting part and provides negative pressure, then the sample can enter the enrichment cavity from the blocking piece, and in the process that the sample flows in, a formed object stays on the blocking piece to form a retentate, so that the enrichment operation of the formed object in the sample is completed.
Preferably, the method further comprises the step of blowing air before suction: operating the enrichment power plant to provide positive pressure to allow gas to enter the sample container.
The air blowing operation can enable the sample in the sample container to flow and turn over up and down, so that solid substances or sediments in the sample can float upwards, and the enrichment operation is more facilitated.
Preferably, the enriching device provides the enriched sample for detection specifically as follows: the enricher is separated from the sample container and moved to spot the enriched sample onto a carrier for detection.
After the sample is enriched on the enricher, the enricher and the sample container need to be separated, and then the enricher is moved to perform spotting.
Because the enrichment process is to enrich solid substances or precipitates in the sample, the sample enriched on the barrier piece of the enrichment device is sticky and is not easy to fall off, and therefore, the enrichment power device only needs to continuously provide negative pressure in the enrichment operation process, so that the sample can be enriched on the barrier piece; after the enrichment operation is finished, the negative pressure can be stopped being provided, and the sample on the blocking piece can not fall off.
Preferably, the enrichment power device moves the spotted concentrator to above the sample container, then disengages the concentrator from the enrichment power device, combines the spotted concentrator with the sample container or is accommodated by the sample container, and then the concentrator moves together with the sample container and is discarded.
Separating the enricher from the sample container, and moving the enricher to perform sample application; and after the sample application is finished, the concentrator is moved to the position above the sample container, so that the concentrator is separated from the enrichment power device, and the concentrator after the sample application is combined with the sample container or accommodated by the sample container and then discarded together. The enricher is combined with the sample container or is contained by the sample container, the enricher can be directly discarded above the sample container by an enrichment power device, and the enricher freely falls and is clamped at the opening of the sample container or enters the interior of the sample container; or the enrichment power device moves downwards, and the enrichment device is placed in the sample container or is clamped (or screwed) at the opening of the sample container.
Preferably, the printing is in particular press printing.
Spotting is preferably a push-type spotting, i.e., moving the concentrator up and down over the carrier, leaving the sample on the carrier for detection. In addition, smear spotting is also possible, i.e., the enricher moves horizontally while contacting the carrier surface, leaving the sample on the carrier.
An apparatus for processing a sample using the sample processing method of any one of the above, comprising: an enrichment power device 1, a sample conveying device 2 and a workpiece conveying device 3,
the enrichment power device 1 is used for connecting an enricher, providing sample enrichment power and driving the enricher to move;
the sample transport device 2 is used for transporting sample containers;
the work piece transport device 3 is used to transport the enricher.
The application also provides a device for processing samples by using the sample processing method, which is moved by the enrichment power device 1, one of the enrichers transported by the workpiece transport device 3 is connected to the enrichment power device 1, is moved to a certain sample container transported by the sample transport device 2, is combined with the sample container, and then starts the enrichment power device 2 to perform enrichment operation; after enrichment, the enrichment power device 1 moves the enricher to separate from the sample container, and samples are applied to the carrier; after sample application, the enricher is moved to the position above the original sample container for enriching the sample, so that the enricher is separated from the enrichment power device 1, combined with the original sample container or accommodated by the original sample container, then the sample container and the enricher which are together are continuously moved by the sample transportation device 2 to be discarded, and meanwhile, the sample transportation device 2 moves a new sample container to carry out next detection.
The sample processing device provided by the application can be used together with a detection device, a carrier supply device and the like to form a detection instrument.
Example 1
A method of sample processing comprising the steps of:
enriching a sample in a sample container using an enricher in combination with the sample container; the enrichment device comprises an enrichment cavity surrounded by an enrichment shell, a blocking piece and a suction connecting part, wherein the blocking piece is respectively connected with the enrichment shell; the enrichment step specifically comprises the following steps: connecting the suction connection to an enrichment power unit and immersing the barrier in the sample; operating the enrichment power device to provide negative pressure, so that the sample is sucked into the enrichment cavity, and meanwhile, the tangible matters in the sample are retained on the barrier to form a retentate;
allowing the concentrator to provide an enriched sample for detection; the enriching device provides the enriched sample for detection specifically as follows: the enricher is separated from the sample container and moves, and the enriched sample is pressed and spotted to a carrier for detection; the enrichment power device moves the concentrator after sample application to the upper part of the sample container, then the concentrator is separated from the enrichment power device, the concentrator after sample application is combined with the sample container or is contained by the sample container, and then the concentrator and the sample container move together to be discarded.
Further comprising the step of blowing air prior to suction: operating the enrichment power plant to provide positive pressure to allow gas to enter the sample container.
Example 2
A method of sample processing, the same as in example 1, except that the method further comprises the steps of diluting the sample prior to enrichment and mixing the sample after dilution.
Example 3
An apparatus for processing a sample, comprising: an enrichment power device 1, a sample conveying device 2 and a workpiece conveying device 3,
the enrichment power device 1 is used for connecting an enricher, providing sample enrichment power and driving the enricher to move;
the sample transport device 2 is used for transporting sample containers;
the work piece transport device 3 is used to transport the enricher.
A sample processing method using the device, comprising the steps of:
the sample in the sample container is enriched by using the enriching device conveyed by the workpiece conveying device 3 and the sample container conveyed by the sample conveying device 2; the enrichment device comprises an enrichment cavity surrounded by an enrichment shell, a blocking piece and a suction connecting part, wherein the blocking piece is respectively connected with the enrichment shell; the enrichment step specifically comprises the following steps: connecting the suction connection of the concentrator to an enrichment power unit 1 and immersing the barrier in the sample; operating the enrichment power device 1 to provide negative pressure, so that the sample is sucked into the enrichment cavity, and meanwhile, the tangible matters in the sample are retained on the barrier to form a retentate;
allowing the concentrator to provide an enriched sample for detection; the enriching device provides the enriched sample for detection specifically as follows: moving an enrichment power device 1 to separate and move the enricher from the sample container, and pressing and spotting the enriched sample to a carrier for detection; the enrichment power device 1 moves the concentrator after sample application to the top of the sample container, then makes the concentrator break away from the enrichment power device 1, makes the concentrator after sample application combine with the sample container or be held by the sample container, then the concentrator with the sample container is moved together by the sample transportation device 2 and is abandoned.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (10)
1. A method of sample processing, comprising the steps of:
enriching a sample in a sample container using an enricher in combination with the sample container;
allowing the concentrator to provide an enriched sample for detection;
discarding the concentrator along with the sample container.
2. The method of claim 1, wherein the step of discarding the concentrator together with the sample container is embodied as: the spotted concentrator is combined with or contained by the sample container, and then moved with the sample container to be discarded.
3. The method of claim 1, further comprising the step of diluting the sample prior to enrichment.
4. The method of claim 3, further comprising the step of blending the sample after dilution.
5. The sample processing method of any of claims 1-4, wherein the concentrator comprises an enrichment chamber surrounded by an enrichment housing, a barrier and a suction connection respectively connected to the enrichment housing;
the enrichment step specifically comprises the following steps:
connecting the suction connection to an enrichment power unit and immersing the barrier in the sample;
and operating the enrichment power device to provide negative pressure, so that the sample is sucked into the enrichment cavity, and meanwhile, the tangible matters in the sample are retained on the barrier to form a retentate.
6. The sample processing method of claim 5, further comprising the step of blowing air prior to aspirating: operating the enrichment power plant to provide positive pressure to allow gas to enter the sample container.
7. The sample processing method of claim 5, wherein the enriching device provides the enriched sample for detection specifically as: the enricher is separated from the sample container and moved to spot the enriched sample onto a carrier for detection.
8. The sample processing method of claim 7, wherein the enrichment motive means moves the spotted concentrator above the sample container and then disengages the concentrator from the enrichment motive means, allowing the spotted concentrator to be combined with or contained by the sample container, and then the concentrator moves with the sample container and is discarded.
9. Sample processing method as claimed in claim 7, characterized in that the printing is in particular press printing.
10. An apparatus for processing a sample using the sample processing method according to any one of claims 5 to 9, comprising: an enrichment power device (1), a sample conveying device (2) and a workpiece conveying device (3),
the enrichment power device (1) is used for connecting the enrichment device, providing sample enrichment power and driving the enrichment device to move;
the sample transport device (2) is used for transporting sample containers;
the workpiece conveying device (3) is used for conveying the enricher.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010750703.1A CN114062101A (en) | 2020-07-30 | 2020-07-30 | Sample processing method and device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010750703.1A CN114062101A (en) | 2020-07-30 | 2020-07-30 | Sample processing method and device |
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| CN114062101A true CN114062101A (en) | 2022-02-18 |
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| CN202010750703.1A Pending CN114062101A (en) | 2020-07-30 | 2020-07-30 | Sample processing method and device |
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| US5188946A (en) * | 1989-01-26 | 1993-02-23 | Biocontrol Systems Incorporated | Process and device for sequential microbial enrichment in a single apparatus |
| CN206311561U (en) * | 2016-12-28 | 2017-07-07 | 力合科技（湖南）股份有限公司 | A kind of monitoring system |
| US20180321128A1 (en) * | 2017-05-02 | 2018-11-08 | See-Through Scientific Limited | Fluid Sample Enrichment System and Method |
| CN110283792A (en) * | 2019-07-04 | 2019-09-27 | 深圳出入境检验检疫局动植物检验检疫技术中心 | A kind of efficiently concentrating method of plant virus |
| CN110836794A (en) * | 2018-08-16 | 2020-02-25 | 湖南爱威医疗科技有限公司 | Sample manufacturing method |
| CN111044514A (en) * | 2019-11-27 | 2020-04-21 | 爱威科技股份有限公司 | Enrichment method, sample manufacturing method and sample detection method |
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2020
- 2020-07-30 CN CN202010750703.1A patent/CN114062101A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5188946A (en) * | 1989-01-26 | 1993-02-23 | Biocontrol Systems Incorporated | Process and device for sequential microbial enrichment in a single apparatus |
| CN206311561U (en) * | 2016-12-28 | 2017-07-07 | 力合科技（湖南）股份有限公司 | A kind of monitoring system |
| US20180321128A1 (en) * | 2017-05-02 | 2018-11-08 | See-Through Scientific Limited | Fluid Sample Enrichment System and Method |
| CN110836794A (en) * | 2018-08-16 | 2020-02-25 | 湖南爱威医疗科技有限公司 | Sample manufacturing method |
| CN110283792A (en) * | 2019-07-04 | 2019-09-27 | 深圳出入境检验检疫局动植物检验检疫技术中心 | A kind of efficiently concentrating method of plant virus |
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