CN114052013A - Medicament composition for preventing and treating powdery mildew of tobacco - Google Patents
Medicament composition for preventing and treating powdery mildew of tobacco Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/24—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing ingredients to enhance the sticking of the active ingredients
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/64—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
- A01N43/647—Triazoles; Hydrogenated triazoles
- A01N43/653—1,2,4-Triazoles; Hydrogenated 1,2,4-triazoles
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Abstract
The invention provides a medicament composition for preventing and treating tobacco powdery mildew, which is prepared from the following raw materials in dilution times: 500 times of polyhydroxy fatty acid ester and 12.5% myclobutanil microemulsion 1000-1500 times of liquid. According to the invention, by adding the polyhydroxyalkanoate polymer material, the sprayed medicine drops can form a polymer film on the surface of the target, the viscosity of the medicine liquid can be increased, the adhesive force of the medicine liquid on the target can be improved, rain wash can be effectively prevented, the release of pesticide can be effectively controlled, the field lasting period of myclobutanil can be further prolonged, the application times can be reduced, the pesticide residue can be reduced, and the pollution of the pesticide to the environment can be reduced.
Description
Technical Field
The invention belongs to the technical field of pesticides, and particularly relates to a medicament composition for preventing and treating tobacco powdery mildew.
Background
Tobacco is one of important economic crops in China, the planting area and the total yield of the tobacco are in the first place in the world, and the tobacco is still affected by plant diseases. The tobacco powdery mildew is used as a main leaf fungal disease, both the seedling stage and the field stage can occur, in a short time, the powdery mildew can cover most or all leaves, the seriously ill leaves lose the baking value, the less ill leaves are diseased, in the modulation process, the disease spots can still continue to be enlarged, the leaves are thin like paper, are easy to break into paste, lack elasticity and lose economic value.
At present, the powdery mildew prevention and treatment in China mainly adopts pesticide and water for dilution and direct spraying, but because of climate change, rainfall increase, nonstandard use of pesticide and other factors, the resistance of germs to the pesticide is increased, such as thiophanate-methyl, triadimefon and the like, and the effective prevention and treatment effect is difficult to achieve by continuously using the pesticide for a long time. The existing control method mainly has the following defects: the emergence period and the outbreak period (6-7 months) of powdery mildew are the period of frequent occurrence in rainy season, the rainfall is frequent, and the optimal control period of pesticide application is not easy to master, thus causing difficult control of the powdery mildew. Most of the pesticides for preventing and treating powdery mildew are non-systemic, and the scour resistance of the pesticide is poor. The pesticide is washed by rainwater, so that the pesticide loss is large. When the pesticide is used repeatedly, the tobacco is easy to generate drug resistance, the use period of the pesticide is reduced, the commodity of the pesticide is reduced, the pesticide effect cannot be effectively exerted, and the control effect on powdery mildew is poor. But also increases the residual quantity of pesticide and the pollution to the environment.
Disclosure of Invention
The technical problem to be solved by the invention is to provide a medicament composition for preventing and treating tobacco powdery mildew by adding polyhydroxyalkanoate, which can increase the viscosity of a liquid medicament, improve the adhesive force of the liquid medicament on a target, effectively prevent rain wash and control the release of pesticide, further prolong the field lasting period of myclobutanil, reduce the application times, reduce the pesticide residue and reduce the pollution of the pesticide to the environment.
In order to solve the technical problems, the invention adopts the technical scheme that: a medicament composition for preventing and treating tobacco powdery mildew is prepared from the following raw materials: the preparation method is characterized in that 12.5% myclobutanil microemulsion is diluted by 1000-1500 times of solution in 500 times of solution of polyhydroxyalkanoate to obtain the myclobutanil microemulsion.
Preferably, the feed is prepared from the following raw materials: the preparation method is characterized in that 12.5% myclobutanil microemulsion is prepared by diluting the 12.5% myclobutanil microemulsion by 1000 times of liquid in 500 times of liquid polyhydroxyalkanoate.
Preferably, the 12.5% myclobutanil microemulsion is prepared by diluting the 12.5% myclobutanil microemulsion by 1500 times in 500 times of polyhydroxyalkanoate.
Preferably, the preparation method of the 500-time liquid polyhydroxyalkanoate comprises the following steps:
weighing 0.1kg of polyhydroxyalkanoate raw material, adding 1kg of water with the temperature of 50 ℃, mixing and stirring to obtain liquid A, weighing 0.5kg of alkylphenol polyoxyethylene ether raw material, adding the alkylphenol polyoxyethylene ether raw material into 49kg of water, uniformly mixing to obtain liquid B, adding the liquid B into the liquid A until the polyhydroxyalkanoate raw material is completely dissolved, and filtering to obtain polyhydroxyalkanoate with the volume of 500 times; the content of the effective components of the polyhydroxy fatty acid ester raw material is 99%; the content of effective components in the alkylphenol polyoxyethylene raw material is 99%; the polyhydroxy fatty acid ester raw material is purchased from Franto Youmann science and technology Co., Ltd; the alkylphenol polyoxyethylene raw material is purchased from the market and is purchased from the Heian petrochemical plant of Jiangsu province.
Compared with the prior art, the invention has the following advantages:
according to the invention, the polyhydroxy fatty acid ester and the myclobutanil are mixed, and the polyhydroxy fatty acid ester is added, so that the viscosity of the liquid medicine can be increased, the adhesive force of the liquid medicine on a target is improved, the particle size of spray droplets can be adjusted, the performance of the spray liquid is improved, and the liquid medicine has the characteristic of film forming, can form a film similar to a raincoat on the surface of a target crop, and can resist the erosion of rainwater. Therefore, the rainfall is frequent in the positive rainy season in the outbreak period of powdery mildew (6-7 months), the optimal control period can be grasped according to weather forecast, better drug effect can be ensured as long as the rainfall is not in 2-3h after the drug is applied, higher control effect can be kept without repeated application even if the rainfall with medium intensity is encountered later, and in addition, the release of the pesticide can be controlled, the field duration of the myclobutanil can be prolonged, the application times can be reduced, the pesticide residue can be reduced, and the pollution of the pesticide to the environment can be reduced.
The present invention will be described in further detail with reference to the accompanying drawings and examples.
Drawings
FIG. 1 is a dynamic graph showing the change in the number of small spots in example 1 of the present invention.
FIG. 2 is a dynamic graph showing the change in the number of large lesions in example 1 of the present invention.
Detailed Description
The 12.5% myclobutanil microemulsion of each of the following examples was purchased from agro-gaku gmbh, n.p. Hainan;
the preparation method of the polyhydroxyalkanoate with 500 times of liquid comprises the following steps:
weighing 0.1kg of polyhydroxyalkanoate raw material, adding 1kg of water with the temperature of 50 ℃, mixing and stirring to obtain liquid A, weighing 0.5kg of alkylphenol polyoxyethylene ether raw material, adding the alkylphenol polyoxyethylene ether raw material into 48.9kg of water, uniformly mixing to obtain liquid B, adding the liquid B into the liquid A until the polyhydroxyalkanoate raw material is completely dissolved, and filtering to obtain polyhydroxyalkanoate of 500 times liquid; the content of the effective components of the polyhydroxy fatty acid ester raw material is 99 percent; the content of effective components in the alkylphenol polyoxyethylene raw material is 99 percent;
polyhydroxy fatty acid ester raw material, commercially available from santa province, mercy, chemmann technologies ltd;
alkylphenol polyoxyethylene raw materials are purchased from commercial suppliers and are purchased from Haian petrochemical plants in Jiangsu province.
Example 1
This example is an indoor bioassay of 12.5% myclobutanil microemulsion and polyhydroxyalkanoates for tobacco powdery mildew (lesion) inhibition.
Flue-cured tobacco variety: nanjiang No. 3 flue-cured tobacco.
Test germs: erysiphe cichororaceae (Erysiphe cichoracearum).
Test site: greenhouse for agricultural institutions of Guizhou province.
Reagent to be tested:
treatment 1: diluting 12.5% myclobutanil micro-emulsion into 500 times of liquid with water for spraying;
and (3) treatment 2: diluting 12.5% myclobutanil micro emulsion in 500 times of liquid polyhydroxy fatty acid ester, diluting into 500 times of liquid, and spraying.
And (3) treatment: diluting 12.5% myclobutanil micro-emulsion into 1000 times of liquid by water for spraying;
and (4) treatment: diluting 12.5% myclobutanil micro emulsion in 500 times of polyhydroxy fatty acid ester, diluting into 1000 times of solution, and spraying.
And (4) treatment 5: diluting 12.5% myclobutanil micro-emulsion into 1500 times of solution with water for spraying;
and (6) treatment: diluting 12.5% myclobutanil microemulsion in 500 times of polyhydroxy fatty acid ester, diluting into 1500 times of solution, and spraying;
and (7) treatment: diluting 12.5% myclobutanil micro-emulsion into 2000 times of solution with water for spraying;
and (4) treatment 8: diluting 12.5% myclobutanil micro emulsion in 500 times of polyhydroxy fatty acid ester, diluting to 2000 times of solution, and spraying.
And (4) treatment: diluting 12.5% myclobutanil micro-emulsion into 2500 times of liquid with water for spraying;
and (4) treatment 10: diluting 12.5% myclobutanil micro emulsion in 500 times of polyhydroxy fatty acid ester, diluting to 2500 times of solution, and spraying.
And (3) experimental design: the tobacco powdery mildew on fresh tobacco leaves is brushed into a beaker by a brush pen and diluted by distilled water to a suspension containing 40-50 spores per visual field under a microscope of 10X 10. Uniformly spraying the conidium suspension on leaves of tobacco seedlings by using a sprayer to inoculate powdery mildew, selecting 1 leaf of tobacco leaves at the middle upper part of each tobacco seedling to inoculate, spraying a treatment agent after 7 days, investigating the number of disease spots for 1 time every 3 days, and continuously investigating for 1 month. 5 cigarettes were observed for each treatment, 3 replicates.
The basis for classifying the lesion is as follows: large disease spots: the diameter is more than 0.5 cm; small scab: the diameter is below 0.5 cm.
The reduction rate of the disease spots is (number of disease spots before medicine-number of disease spots at investigation)/number of disease spots before medicine x 100%
As can be seen from the dynamic change of the number of small lesions in fig. 1, the number of small lesions in treatment 1, treatment 2 and treatment 3 was reduced to 0 at 9d, and rebound occurred at 30d, 27d and 27d, respectively; the number of small lesions in treatments 6, 7 and 9 decreased to 0 at 12d, with rebound at 24d, 21d and 21d, respectively; treatment 8 decreased to 0 at 15d and rebound occurred in small lesions at 21 d; the number of small plaques in 12d after treatment 10 administration continued to decrease but did not decrease to 0. Compared with single-dose treatment of myclobutanil, the mixed treatment of the polyhydroxyalkanoate and the myclobutanil can effectively prolong the control time of small scab. Wherein, the number of small spots of treatment 4 and treatment 6 is reduced to 0 at the 9 th day, and the number of small spots does not rebound in the whole observation period, and the control period of the small spots reaches 27 days.
As can be seen from the dynamic change of the number of large lesions in FIG. 2, the number of large lesions in treatments 1, 3 and 5 was reduced to 0 at 15d, 12d and 12d, and all of them appeared rebounding at 30 d; treatment 8 decreased to 0 at 15d and rebound for large lesions at 27 d; the number of large lesions in 15d, 24d after treatment 7, treatment 9 and treatment 10 administration continued to decrease but did not decrease to 0. Among them, the number of large lesions in treatments 2, 4 and 6 was reduced to 0 at 15d, 12d and 12d, and there was no rebound of the number of large lesions throughout the observation period. In comprehensive comparison, treatments 4 and 6 gave the best control of powdery mildew.
Example 2
This example shows the indoor tests of 12.5% myclobutanil microemulsion and polyhydroxyalkanoates for controlling powdery mildew of tobacco.
Flue-cured tobacco variety: nanjiang No. 3 flue-cured tobacco.
Reagent to be tested:
treatment 1: diluting 12.5% myclobutanil micro-emulsion into 1000 times of liquid by water for spraying;
and (3) treatment 2: diluting 12.5% myclobutanil microemulsion in 500 times of polyhydroxy fatty acid ester, diluting into 1000 times of solution, and spraying;
and (3) treatment: diluting 12.5% myclobutanil micro-emulsion into 1500 times of solution with water for spraying;
and (4) treatment: diluting 12.5% myclobutanil microemulsion in 500 times of polyhydroxy fatty acid ester, diluting into 1500 times of solution, and spraying;
and (4) treatment 5: diluting 12.5% myclobutanil micro-emulsion into 2000 times of solution with water for spraying;
and (6) treatment: diluting 12.5% myclobutanil microemulsion in 500 times of polyhydroxy fatty acid ester, diluting into 2000 times of solution, and spraying;
and (7) treatment: and (5) clear water control.
The test method comprises the following steps: the tobacco powdery mildew on fresh tobacco leaves is brushed into a beaker by a brush pen and diluted by distilled water to a suspension containing 40-50 spores per visual field under a microscope of 10X 10. Uniformly spraying the conidium suspension on leaves of tobacco seedlings by using a sprayer to inoculate powdery mildew, selecting 1 tobacco leaf for each tobacco seedling to inoculate on the middle-upper tobacco leaves, and spraying a treatment agent after 7 days. Spraying according to the divided cells and the corresponding treatment agent, and pressurizing and spraying by using a manual sprayer until all the leaves are wetted. Spraying clear water for one time according to the rainfall simulation of the medium rain after spraying for 3 hours, wherein the daily rainfall of the medium rain is between 10mm and 24.9mm, connecting a water pipe provided with a high-pressure spray head on a water faucet, opening the water faucet, rotating a rotary valve of the spray head to a proper position to spray water, enabling the spray head to spray upwards, enabling falling water drops to be similar to the size of raindrops, and stopping spraying water after a weighing bottle for collecting the rainwater is full (equivalent to the rainfall of 20 mm/d). The disease onset was examined 5d after the administration, and the total of 7 times was examined every 5 d. 5 tobacco plants were observed for each treatment, 3 replicates.
According to the classification standard of the severity of the powdery mildew disease of tobacco in GB/T23222-2008, the flue-cured tobacco in the outbreak area is subjected to fixed-point and plant-fixing investigation, and the classification standard is as follows: level 0: no disease spots; level 1: the leaf area of the scab accounts for less than 5 percent of the leaf area; and 3, level: the leaf area of the scab accounts for 6 to 10 percent of the leaf area; and 5, stage: the area of the diseased leaf accounts for 11-20 percent of the area of the leaf; and 7, stage: the leaf area of the scab accounts for 21-40 percent of the leaf area; and 9, stage: the area of the diseased leaf is more than 41 percent of the area of the leaf, and even pathogenic bacteria are distributed on the whole leaf.
The disease index formula is as follows:
wherein: CK0 is disease index before drug administration in the blank control area, and CK1 is disease index after drug administration in the blank control area; PT0 is disease index before drug administration in drug treatment area; PT1 is the disease index after administration to the agent treatment area.
TABLE 1 prevention after simulation of rainwash
Note: "-" indicates no investigation.
As can be seen from Table 1, the control effect of the treatments 2, 4 and 6 is better than that of the single-dose treatment of myclobutanil on the 3 rd, 7 th, 14 th, 21 th and 28 th days after water spraying, wherein the control effect of the treatments 2 and 4 on the 28 th day is still as high as 81.89% and 78.79%. Therefore, the polyhydroxy fatty acid ester and the myclobutanil are mixed to effectively resist the erosion of rainwater, reduce the loss of pesticide and prolong the field lasting period of the pesticide.
Example 3
This example is a test of the deposition amount and leaching rate of pesticide on tobacco leaves after rainwash of 12.5% myclobutanil microemulsion and polyhydroxyalkanoate.
Flue-cured tobacco variety: nanjiang No. 3 flue-cured tobacco. And (4) raising the seedlings according to a floating plate seedling raising method, and transplanting the tobacco seedlings into a greenhouse for later use after 7-8 main leaves are cultured.
Treatment 1: diluting 12.5% myclobutanil micro-emulsion into 1500 times of solution with water for spraying;
and (3) treatment 2: diluting 12.5% myclobutanil microemulsion in 500 times of polyhydroxy fatty acid ester, diluting into 1500 times of solution, and spraying;
and (3) treatment: diluting 12.5% myclobutanil micro-emulsion into 1000 times of liquid by water for spraying;
and (4) treatment: diluting 12.5% myclobutanil micro emulsion in 500 times of polyhydroxy fatty acid ester, diluting into 1000 times of solution, and spraying.
The tobacco powdery mildew on fresh tobacco leaves is brushed into a beaker by a brush pen and diluted by distilled water to a suspension containing 40-50 spores per visual field under a microscope of 10X 10. Uniformly spraying the conidium suspension on leaves of tobacco seedlings by using a sprayer to inoculate powdery mildew, selecting 1 tobacco leaf for each tobacco seedling to inoculate on the middle-upper tobacco leaves, and spraying a treatment agent after 7 days. The spray was pressurized using a hand sprayer until all the leaves were wetted. Spraying clear water for one time according to the rainfall simulation of the medium rain after spraying for 3 hours, wherein the daily rainfall of the medium rain is between 10mm and 24.9mm, connecting a water pipe provided with a high-pressure spray head on a water faucet, opening the water faucet, rotating a rotary valve of the spray head to a proper position to spray water, enabling the spray head to spray upwards, enabling falling water drops to be similar to the size of raindrops, and stopping spraying water after a weighing bottle for collecting the rainwater is full (equivalent to the rainfall of 20 mm/d). Sampling was performed before and after spraying water (immediately after the leaf surface was dropped dry), and at 7d, 14d, and 21d after spraying water, respectively, and 5 cigarettes were randomly picked for each treatment, and 3 times repeated. And (5) delivering the product to a quality supervision and inspection station of pesticide products in Guizhou province for pesticide residue inspection.
The pesticide residue detection method comprises the following steps:
extraction and purification by a QuEChERS method: accurately weighing 5.0g (accurate to 0.02g) of crushed dry tobacco leaf sample, adding 25mL of acetonitrile, ultrasonically extracting for 30min, and standing for 30 min; taking 0.75mL of supernatant, putting the supernatant into a 2.0mL centrifuge tube containing 150mg of anhydrous magnesium sulfate, 50mg of N-propyl ethylenediamine (PSA) and 10mg of Graphite Carbon Black (GCB), adding 0.75mL of ultrapure water, carrying out vortex for 1min, and centrifuging for 5min at 5000 rpm; taking the supernatant to pass through a 0.22 mu m organic filter membrane to be detected.
Liquid chromatography conditions: chromatography column, Hypersil GOLD C18 column, 100mm × 2.1mm × 1.7 μm. Column temperature, 40 ℃. Mobile phase a was 0.1% formic acid water and mobile phase B was acetonitrile. Binary gradient elution, 0-0.5min, 20% B; 0.50-3.0min, 20% -90% B; 3.0-7.0min, 90% -98% B; 7.0-7.5min, 98% -20% B; 7.5-10.0min, 20% B. Flow rate: 0.2 mL/min; sample introduction amount: 2 μ L.
The mass spectrometry method comprises the following steps: scanning by atmospheric pressure chemical ionization ESI + mode, wherein the temperature of a capillary is 350 ℃, the voltage of electrospray is 3500V, the flow rate of sheath gas and auxiliary gas is nitrogen, the flow rate of sheath gas is 35Arb, the flow rate of auxiliary gas is 10Arb, and the collision gas is argon. The S-beans radio frequency voltage is 82V, the collision energy is 18V and 34V respectively, the qualitative ion pair is 289.116m/z/70.028m/z, and the quantitative ion pair is 289.116m/z/125.04 m/z.
Reagent to be tested:
TABLE 2 deposition on tobacco and Leaching Rate after rain erosion
The leaching rates of the pesticides after water spraying in the treatment 1 and the treatment 3 are respectively 90.46% and 92.66%, most of the pesticides are washed away by rainwater, and the pesticide residue rates 7 days after water spraying are 8.77% and 7.70%; the leaching loss rates after the water spraying of the treatments 2 and 4 are respectively 28.09% and 28.24%, most of the pesticide is still remained, the pesticide residue rates after the water spraying of the treatments 2 and 4 are respectively 55.2% and 45.57% which are far higher than those of the treatments 1 and 3, and the pesticide residues 14d and 21d after the water spraying are also higher than those of the treatments 1 and 3. The anti-rainwash effect of treatments 2 and 4 was significant.
Example 4
This example is a field trial of 12.5% myclobutanil microemulsion and polyhydroxyalkanoates for controlling tobacco powdery mildew.
Flue-cured tobacco variety: nanjiang No. 3 flue-cured tobacco.
Test site: cross country cross village in flat dam area of Anshun city
Treatment 1: diluting 12.5% myclobutanil micro-emulsion into 1500 times of solution with water for spraying;
and (3) treatment 2: diluting 12.5% myclobutanil microemulsion in 500 times of polyhydroxy fatty acid ester, diluting into 1500 times of solution, and spraying;
and (3) treatment: diluting 12.5% myclobutanil micro-emulsion into 1000 times of liquid by water for spraying;
and (4) treatment: diluting 12.5% myclobutanil micro emulsion in 500 times of polyhydroxy fatty acid ester, diluting into 1000 times of solution, and spraying.
CK: clear water was used as a blank control.
The test method comprises the following steps: and selecting a field block with a serious tobacco powdery mildew occurrence rate as a test field in the cross village of the flat dam. The time when rainfall occurred on day 2 after application was selected according to the weather forecast to start application (application started on day 25/6/2018), and the weather conditions during the experiment were recorded after application (table 4). Each treatment observation was 30m2And 3 repetitions. The occurrence of powdery mildew was investigated 14 days after application. Each treatment is divided intoA random five-point sampling method is adopted, 5 points are investigated in each treatment, 5 plants are investigated in each point, the number of diseased leaves and the disease degree are investigated, and disease index and prevention and treatment effect are calculated.
According to the classification standard of the severity of the powdery mildew disease of tobacco in GB/T23222-2008, the flue-cured tobacco in the outbreak area is subjected to fixed-point and plant-fixing investigation, and the classification standard is as follows: level 0: no disease spots; level 1: the leaf area of the scab accounts for less than 5 percent of the leaf area; and 3, level: the leaf area of the scab accounts for 6 to 10 percent of the leaf area; and 5, stage: the area of the diseased leaf accounts for 11-20 percent of the area of the leaf; and 7, stage: the leaf area of the scab accounts for 21-40 percent of the leaf area; and 9, stage: the area of the diseased leaf is more than 41 percent of the area of the leaf, and even pathogenic bacteria are distributed on the whole leaf.
The disease index formula is as follows:
wherein: CK0 is disease index before drug administration in the blank control area, and CK1 is disease index after drug administration in the blank control area; PT0 is disease index before drug administration in drug treatment area; PT1 is the disease index after administration to the agent treatment area.
TABLE 3 relative prevention effect after 14 days
As can be seen from table 3, treatment 4 performed best at 78.07% relative control effect, 16.15% higher than treatment 3, given 1 application over the entire test period; the relative control effect of treatment 2 was 72.67% which was 20.57% higher than that of treatment 1. Compared with a single myclobutanil preparation, the polyhydroxy fatty acid ester and myclobutanil mixed combination can effectively resist the scouring action of rainwater, and further can improve the control effect on powdery mildew. Wherein the relative control effect of treatment 2 was 10.75% higher than that of treatment 3. The invention can effectively improve the utilization rate of myclobutanil, resist rain wash and reduce the usage amount of pesticide.
TABLE 4 rainfall during the test
| Date | Conditions of rainfall | Remarks for note |
| 6 month and 25 days | Cloudy/cloudy | 24℃/19℃ |
| 6 month and 26 days | Rain shower/rain shower | 25℃/19℃ |
| 6 months and 27 days | Rain shower/rain shower | 25℃/19℃ |
| 6 month and 28 days | Yin/yin | 26℃/20℃ |
| 6 months and 29 days | Yin/yin | 28℃/20℃ |
| 6 months and 30 days | Rain shower/rain shower | 29℃/21 |
| 7 month and 1 day | Cloudy/cloudy | 27℃/20 |
| 7 month and 2 days | Cloudy/cloudy | 30℃/22 |
| 7 month and 3 days | Cloudy/cloudy | 29℃/21 |
| 7 month and 4 days | Cloudy/ |
27℃/22 |
| 7 month and 5 days | Gust/heavy rain | 28℃/22 |
| 7 month and 6 days | Heavy rain-heavy rain/heavy rain-heavy rain | 26℃/20 |
| 7 month and 7 days | Rain in the shade | 26℃/21 |
| 7 month and 8 days | Gusty rain/ |
27℃/20 |
| 7 month and 9 days | Rain shower/rain shower | 25℃/21℃ |
The above description is only for the preferred embodiment of the present invention, and is not intended to limit the present invention in any way. Any simple modification, change and equivalent changes of the above embodiments according to the technical essence of the invention are still within the protection scope of the technical solution of the invention.
Claims (4)
1. The medicament composition for preventing and treating the powdery mildew of tobacco is characterized by being prepared from the following raw materials: the preparation method is characterized in that 12.5% myclobutanil microemulsion is diluted by 1000-1500 times of solution in 500 times of solution of polyhydroxyalkanoate to obtain the myclobutanil microemulsion.
2. The pharmaceutical composition for preventing and treating tobacco powdery mildew according to claim 1, which is prepared from the following raw materials: the preparation method is characterized in that 12.5% myclobutanil microemulsion is prepared by diluting the 12.5% myclobutanil microemulsion by 1000 times of liquid in 500 times of liquid polyhydroxyalkanoate.
3. The pharmaceutical composition for preventing and treating powdery mildew of tobacco as claimed in claim 1, wherein the composition is prepared by diluting 12.5% myclobutanil microemulsion with 1500 times of solution in 500 times of solution of polyhydroxyalkanoate.
4. The pharmaceutical composition for preventing and treating tobacco powdery mildew according to any one of claims 1-3, wherein the preparation method of the 500-fold liquid polyhydroxyalkanoate comprises the following steps:
weighing 0.1kg of polyhydroxyalkanoate raw material, adding 1kg of water with the temperature of 50 ℃, mixing and stirring to obtain liquid A, weighing 0.5kg of alkylphenol polyoxyethylene ether raw material, adding the alkylphenol polyoxyethylene ether raw material into 49kg of water, uniformly mixing to obtain liquid B, adding the liquid B into the liquid A until the polyhydroxyalkanoate raw material is completely dissolved, and filtering to obtain polyhydroxyalkanoate with the volume of 500 times; the content of the effective components of the polyhydroxy fatty acid ester raw material is 99%; the content of active ingredients in the alkylphenol polyoxyethylene raw material is 99%.
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| CN202111529204.0A Active CN114052013B (en) | 2021-12-14 | 2021-12-14 | Pharmaceutical composition for preventing and treating powdery mildew of tobacco |
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| WO2006038631A1 (en) * | 2004-10-05 | 2006-04-13 | Nippon Soda Co., Ltd. | Composition for agricultural use for inhibiting the runoff of pesticides by rain |
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| WO2020242871A1 (en) * | 2019-05-24 | 2020-12-03 | Danimer Bioplastics, Inc. | Pla / pha biodegradable coatings for seeds, fertilizers and pesticides |
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| CN1157551A (en) * | 1994-08-27 | 1997-08-20 | Lts洛曼治疗体系有限公司 | Sprayable film forming system releasing active substances for use on plants |
| CN1444445A (en) * | 2000-06-09 | 2003-09-24 | 宝洁公司 | Agricultural items and methods comprising biodegradable copolymers |
| WO2006038631A1 (en) * | 2004-10-05 | 2006-04-13 | Nippon Soda Co., Ltd. | Composition for agricultural use for inhibiting the runoff of pesticides by rain |
| CN109952283A (en) * | 2016-09-29 | 2019-06-28 | 联邦科学与工业研究组织 | Control release agrochemical compositions |
| WO2020242871A1 (en) * | 2019-05-24 | 2020-12-03 | Danimer Bioplastics, Inc. | Pla / pha biodegradable coatings for seeds, fertilizers and pesticides |
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