CN114042013B - Composition for improving skin care property of hyaluronic acid or salt thereof and method thereof - Google Patents
Composition for improving skin care property of hyaluronic acid or salt thereof and method thereof Download PDFInfo
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- CN114042013B CN114042013B CN202111320857.8A CN202111320857A CN114042013B CN 114042013 B CN114042013 B CN 114042013B CN 202111320857 A CN202111320857 A CN 202111320857A CN 114042013 B CN114042013 B CN 114042013B
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
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- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
- A61K8/922—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of vegetable origin
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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Abstract
The invention discloses a composition for improving skin care performance of hyaluronic acid or salt thereof and a method thereof, wherein the method comprises the following steps: mixing an oil phase and a hyaluronic acid or a salt water phase solution thereof to obtain an emulsion, wherein the oil phase is triglyceride with fully alkylated side chains, the viscosity of the oil phase is less than 85 centipoise, and the mass percentage of the oil phase in the emulsion is 45-75%. The method and the composition can improve the moisture retention of the hyaluronic acid or the salt thereof, namely reduce the negative influence of water on the moisture retention of the hyaluronic acid or the salt thereof, thereby fully exerting the moisture retention performance of the hyaluronic acid or the salt thereof and improving the skin barrier function.
Description
Technical Field
The invention relates to the technical field of hyaluronic acid, in particular to a composition for improving skin care performance of hyaluronic acid or salt thereof and a method thereof.
Background
Hyaluronic acid or its salts are natural skin moisturizers, which for cosmetic applications must be dissolved in an aqueous phase. However, the water molecules themselves have a negative effect on the moisture retention of the skin, i.e. the water molecules bind to the skin keratins, causing the keratinocytes to swell and thus loosening the brick wall structure of the skin. Therefore, the moisturizing and skin-protecting properties of hyaluronic acid or a salt thereof cannot be exhibited to the maximum extent.
Disclosure of Invention
In view of the above problems, the present invention provides a method and a composition for improving skin care properties of hyaluronic acid or a salt thereof, which can reduce the negative effects of water on the moisture retention of hyaluronic acid or a salt thereof and sufficiently exert the moisture retention properties of hyaluronic acid or a salt thereof.
The specific technical scheme of the invention is as follows:
1. a method of improving the skin care properties of hyaluronic acid or a salt thereof, comprising:
mixing an oil phase and a hyaluronic acid or salt water phase solution to obtain an emulsion, wherein the oil phase is triglyceride with fully alkylated side chains, the viscosity of the oil phase is less than 85 centipoises, and the mass percentage of the oil phase in the emulsion is 45-75%.
2. The method of item 1, wherein the oil phase is 50-70% by mass in the emulsion.
3. The method of item 1 or 2, wherein the viscosity of the oil phase is 30-85 centipoise.
4. The method according to any one of claims 1-3, wherein the oil phase is selected from one or more of meadowfoam seed oil, olive oil and caprylic/capric triglyceride.
5. The method according to any one of claims 1 to 4, wherein the hyaluronic acid or a salt thereof is one or more selected from the group consisting of hyaluronic acid, sodium hyaluronate, potassium hyaluronate, zinc hyaluronate, magnesium hyaluronate, calcium hyaluronate, and gold hyaluronate, preferably sodium hyaluronate; preferably, the molecular weight of the hyaluronic acid or the salt thereof is more than or equal to 1w Da, and preferably 1w-200w Da.
6. A composition for improving the skin care performance of hyaluronic acid or a salt thereof comprises hyaluronic acid or a salt thereof and an oil phase, wherein the oil phase is triglyceride with fully alkylated side chains, the viscosity of the oil phase is less than 85 centipoise, and the mass percentage of the oil phase in the composition is 45-75%.
7. The composition according to item 6, wherein the oil phase is 50-70% by mass of the composition.
8. The composition of item 6 or 7, wherein the viscosity of the oil phase is 30-85 centipoise.
9. The composition according to any one of claims 6 to 8, wherein the oil phase is selected from one or more of meadowfoam seed oil, olive oil and caprylic/capric triglyceride.
10. The composition according to any one of claims 6 to 9, wherein the hyaluronic acid or a salt thereof is one or more selected from hyaluronic acid, sodium hyaluronate, potassium hyaluronate, zinc hyaluronate, magnesium hyaluronate, calcium hyaluronate, and gold hyaluronate, preferably sodium hyaluronate; preferably, the molecular weight of the hyaluronic acid or the salt thereof is more than or equal to 1w Da, and preferably 1w-200w Da.
11. A method of using the composition of any one of items 6-10, comprising:
preparing hyaluronic acid or salt thereof into an aqueous phase solution, and then mixing the aqueous phase solution with an oil phase to obtain an emulsion;
the resulting emulsion is applied to the desired area of the subject.
12. A method of using the composition of any one of claims 6-10, comprising any one of:
(1) Storing the hyaluronic acid or its salt water phase solution and oil phase in the same container, layering the water phase and oil phase in the container, shaking before use, and pouring out or spraying out;
(2) The hyaluronic acid or salt water phase solution and the oil phase are stored in the same container, the water phase and the oil phase are stored at intervals and partitioned, and the two phases are pumped out according to a certain proportion by a pump head when in use;
(3) Storing the hyaluronic acid or its salt water phase solution and oil phase in different containers for matching use;
(4) The aqueous solution of hyaluronic acid or salt thereof and the oil phase are stored in a single-use package in the form of a disposable or ampoule.
13. Use of a triglyceride, which is a side chain fully alkylated triglyceride having a viscosity of less than 85 centipoise, for improving skin care of hyaluronic acid or a salt thereof.
14. Use of the composition of any one of claims 6-10 in a wet wipe liquor.
ADVANTAGEOUS EFFECTS OF INVENTION
The method and the composition can improve the moisture retention of the hyaluronic acid or the salt thereof, namely reduce the negative influence of water on the moisture retention of the hyaluronic acid or the salt thereof, thereby fully exerting the moisture retention performance of the hyaluronic acid or the salt thereof.
The method and the composition can improve the moisture content of the skin, reduce the excessive swelling of the stratum corneum and avoid the stimulation of a surfactant, thereby improving the barrier function of the skin.
Drawings
FIG. 1 is a graph showing the moisturizing coefficients of subjects after being smeared with water and an aqueous solution of hyaluronic acid or a salt thereof of case-1.
Detailed Description
The present invention is described in detail below. While specific embodiments of the invention have been shown, it should be understood that the invention may be embodied in various forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the invention to those skilled in the art.
It should be noted that certain terms are used throughout the description and claims to refer to particular components. As one skilled in the art will appreciate, various names may be used to refer to a component. The description and claims do not intend to distinguish between components that differ in noun but not in function. In the following description and in the claims, the terms "include" and "comprise" are used in an open-ended fashion, and thus should be interpreted to mean "include, but not limited to. The description which follows is a preferred embodiment of the invention, however, the description is given for the purpose of illustrating the general principles of the invention and not for the purpose of limiting the scope of the invention. The scope of the invention is to be determined by the claims appended hereto.
The invention provides a method for improving the skin care performance of hyaluronic acid or a salt thereof, which comprises the following steps:
mixing an oil phase and a hyaluronic acid or salt water phase solution to obtain an emulsion, wherein the oil phase is triglyceride with fully alkylated side chains, the viscosity of the oil phase is less than 85 centipoises, and the mass percentage of the oil phase in the emulsion is 45-75%.
In order to promote the dissolution of the hyaluronic acid or the salt thereof, the aqueous phase solution of the hyaluronic acid or the salt thereof can also contain a cosolvent, and the cosolvent can be glycerol.
The mixing is physical mixing, for example, the mixing can be in the form of a water-oil two-phase shaker, or in the form of single package with rated proportion or oil-water two-phase products which are used together by separating and packaging according to the set proportion.
The shake flask is a shake flask commonly used in the art, and refers to a flask which has two water phases and two oil phases in the same container, has no emulsifier between the two phases, and the two water phases and the two oil phases are layered, before use, the two water phases and the two oil phases form an emulsion by shaking and shaking, and then the emulsion is poured out for smearing.
The present invention can improve the moisturizing performance of hyaluronic acid or a salt thereof by mixing an oil phase (containing a side chain-fully alkylated triglyceride) having a specific structure with an aqueous solution of hyaluronic acid or a salt thereof.
The viscosity of the oil phase is, for example, 80 centipoise, 75 centipoise, 70 centipoise, 65 centipoise, 60 centipoise, 55 centipoise, 50 centipoise, 45 centipoise, 40 centipoise, 35 centipoise, 30 centipoise, etc.;
the oil phase may be present in the emulsion at 45%, 50%, 55%, 60%, 65%, 70%, 75% by weight or any range therebetween.
In one embodiment, the oil phase is 50-70% by weight of the emulsion.
With respect to the viscosity of the oil phase, which refers to the viscosity before mixing with the aqueous solution of hyaluronic acid or a salt thereof, the present invention is not limited as long as it is less than 85 centipoise, but since the known side chain fully alkylated triglycerides each have a viscosity above 33 centipoise, in one embodiment, the oil phase has a viscosity of 30 to 85 centipoise.
In one embodiment, the oil phase is selected from one or more than two of meadowfoam seed oil, olive oil and caprylic/capric triglyceride, for example, may be selected from one or a combination of two or three thereof, for example, the oil phase may be olive oil or a mixture of olive oil and caprylic/capric triglyceride or a mixture of caprylic/capric triglyceride and meadowfoam seed oil or meadowfoam seed oil.
When the oil phase is a mixture of olive oil and caprylic/capric triglyceride, the mass ratio (m) of olive oil to caprylic/capric triglyceride Olive oil :m Caprylic capric triglyceride ) From 0.1 to 100, for example, from 0.1 to 1, 1.67.
When the oil phase is a mixture of caprylic/capric triglyceride and meadowfoam seed oil, the mass ratio of caprylic/capric triglyceride to meadowfoam seed oil (m) Caprylic capric acid triglyceride :m Potentilla chinensis seed oil ) 1-50, for example 1:1, 5:1, 10.51.
In one embodiment, the hyaluronic acid or salt thereof is selected from one or more of hyaluronic acid, sodium hyaluronate, potassium hyaluronate, zinc hyaluronate, magnesium hyaluronate, calcium hyaluronate, and gold hyaluronate, and preferably is sodium hyaluronate.
The molecular weight of the hyaluronic acid or the salt thereof is more than or equal to 1w Da, and preferably 1w-200w Da.
For example, the hyaluronic acid or salt thereof may have a molecular weight of 1w Da, 5w Da, 10w Da, 20w Da, 50w Da, 100w Da, 200w Da, etc.
In one embodiment, the aqueous phase solution of hyaluronic acid or a salt thereof is a solution in which hyaluronic acid or a salt thereof, glycerin, is dissolved in water, and glycerin is added for better dissolution of hyaluronic acid or a salt thereof in water to form an aqueous phase solution.
The mass percentage of the hyaluronic acid or a salt thereof in the emulsion is not limited in the present invention, and may be selected as needed as long as the effects of the present invention are achieved, for example, the mass percentage of the hyaluronic acid or a salt thereof in the emulsion is not less than 0.01%.
In one embodiment, the method for improving skin care of hyaluronic acid or a salt thereof comprises:
mixing an oil phase and a hyaluronic acid or a salt water phase solution thereof to obtain an emulsion, wherein the oil phase is triglyceride with fully alkylated side chains, the viscosity of the oil phase is less than 85 centipoise, and the mass percentage of the oil phase in the emulsion is 50-70%.
In one embodiment, the method for improving skin care of hyaluronic acid or a salt thereof comprises:
mixing an oil phase and a hyaluronic acid or a salt water phase solution thereof to obtain an emulsion, wherein the oil phase is triglyceride with fully alkylated side chains, the viscosity of the oil phase is 30-85 centipoises, and the mass percentage of the oil phase in the emulsion is 50-70%.
In one embodiment, the method for improving skin care of hyaluronic acid or a salt thereof comprises:
mixing an oil phase and a hyaluronic acid or a salt water phase solution thereof to obtain an emulsion, wherein the oil phase is triglyceride with fully alkylated side chains, the viscosity of the oil phase is 30-85 centipoises, the mass percentage of the oil phase in the emulsion is 50-70%, and the oil phase is selected from one or more than two of white chingma seed oil, olive oil and caprylic/capric triglyceride.
In one embodiment, the method for improving skin care of hyaluronic acid or a salt thereof comprises:
mixing an oil phase and a water phase solution of hyaluronic acid or a salt thereof to obtain an emulsion, wherein the oil phase is triglyceride with fully alkylated side chains, the viscosity of the oil phase is 30-85 centipoises, the mass percentage of the oil phase in the emulsion is 50-70%, the oil phase is selected from one or more than two of white pond flower seed oil, olive oil and caprylic/capric triglyceride, the hyaluronic acid or a salt thereof is selected from one or more than two of hyaluronic acid, sodium hyaluronate, potassium hyaluronate, zinc hyaluronate, magnesium hyaluronate, calcium hyaluronate and gold hyaluronate, preferably the molecular weight of the hyaluronic acid or a salt thereof is more than or equal to 1w Da, preferably 1w-200w Da.
The method of the present invention can improve the coefficient of moisture retention and further improve the skin-care properties of hyaluronic acid or a salt thereof by physically mixing an oil phase with an aqueous phase solution of hyaluronic acid or a salt thereof.
The invention provides a composition for improving the skin care performance of hyaluronic acid or a salt thereof, which comprises hyaluronic acid or a salt thereof and an oil phase, wherein the oil phase is triglyceride with fully alkylated side chains, the viscosity of the oil phase is less than 85 centipoise, and the mass percent of the oil phase in the composition is 35-85%.
In one embodiment, the oil phase is 50-70% by weight of the composition, preferably the oil phase has a viscosity of 30-85 centipoise, and more preferably the oil phase is selected from one or more of meadowfoam seed oil, olive oil and caprylic/capric triglyceride.
With respect to the viscosity of the oil phase, it is meant the viscosity of the oil phase itself, regardless of whether it is combined with hyaluronic acid or a salt thereof.
In one embodiment, the hyaluronic acid or salt thereof is selected from one or more of hyaluronic acid, sodium hyaluronate, potassium hyaluronate, zinc hyaluronate, magnesium hyaluronate, calcium hyaluronate, and gold hyaluronate, preferably sodium hyaluronate, and preferably the hyaluronic acid or salt thereof has a molecular weight of not less than 1w Da, preferably 1w-200w Da.
In one embodiment, the composition for improving the skin care performance of hyaluronic acid or the salt thereof comprises hyaluronic acid or the salt thereof and an oil phase, wherein the oil phase is triglyceride with fully alkylated side chains, the viscosity of the oil phase is less than 85 centipoise, and the mass percent of the oil phase in the composition is 35-85%.
In one embodiment, the composition for improving the skin care performance of hyaluronic acid or the salt thereof comprises hyaluronic acid or the salt thereof and an oil phase, wherein the oil phase is triglyceride with fully alkylated side chains, the viscosity of the oil phase is less than 85 centipoise, and the mass percent of the oil phase in the composition is 50-70%.
In one embodiment, the composition for improving the skin care performance of the hyaluronic acid or the salt thereof comprises the hyaluronic acid or the salt thereof and an oil phase, wherein the oil phase is triglyceride with fully alkylated side chains, the viscosity of the oil phase is 30-85 centipoises, and the mass percentage of the oil phase in the composition is 50-70%.
In one embodiment, the composition for improving the skin care performance of hyaluronic acid or the salt thereof comprises hyaluronic acid or the salt thereof and an oil phase, wherein the oil phase is triglyceride with fully alkylated side chains, the viscosity of the oil phase is 30-85 centipoises, the mass percentage of the oil phase in the composition is 50-70%, and the oil phase is selected from one or more than two of meadowfoam seed oil, olive oil and caprylic-capric triglyceride.
In one embodiment, the composition for improving the skin care performance of the hyaluronic acid or the salt thereof comprises the hyaluronic acid or the salt thereof and an oil phase, wherein the oil phase is triglyceride with fully alkylated side chains, the viscosity of the oil phase is 30-85 centipoises, the mass percentage of the oil phase in the composition is 50-70%, the oil phase is selected from one or more than two of white chinlon oil, olive oil and caprylic capric triglyceride, and the molecular weight of the hyaluronic acid or the salt thereof is more than or equal to 1w Da, preferably 1w-200w Da.
The present invention is not limited to the mass percentage of the hyaluronic acid or the salt thereof in the composition, and those skilled in the art may select the hyaluronic acid or the salt thereof as needed as long as the technical effect is achieved, and in one embodiment, the mass percentage of the hyaluronic acid or the salt thereof in the composition is greater than 0.01%.
The composition of the invention is prepared into emulsion and coated on the required part of a subject, and the moisturizing coefficient is increased, which shows that the composition of the invention can reduce the negative influence of the book on the moisturizing performance of hyaluronic acid or the salt thereof and fully exert the moisturizing performance of hyaluronic acid or the salt thereof.
The present invention provides a method of using the composition described above, comprising:
preparing hyaluronic acid or its salt into water phase solution, mixing with oil phase to obtain emulsion, and applying the emulsion to the desired part of the subject.
The present invention provides a method of using the composition described above, comprising any of:
(1) Storing the hyaluronic acid or its salt water phase solution and oil phase in the same container, layering the water phase and oil phase in the container, shaking before use, and pouring out or spraying out;
(2) The hyaluronic acid or salt water phase solution and the oil phase are stored in the same container, the water phase and the oil phase are stored in a partition mode at intervals, and the two phases are pumped out according to a certain proportion through a pump head when the hyaluronic acid or salt water phase solution and the oil phase solution are used;
(3) Storing the hyaluronic acid or its salt water phase solution and oil phase in different containers, and using in combination;
(4) The aqueous phase solution of hyaluronic acid or salt thereof and the oil phase are stored in a single-use package in the form of a disposable or ampoule.
The hyaluronic acid or its salt water phase solution and oil phase are stored in the same container, the water phase and oil phase are separated in the container, and are shaken before use, poured out or sprayed out, wherein the water phase and oil phase are separated before use.
The hyaluronic acid or salt water phase solution and the oil phase are stored in the same container, the water phase and the oil phase are stored at intervals, and when the hyaluronic acid or salt water phase solution and the oil phase solution are used, the two phases are pumped out by a pump head according to a certain proportion, namely the two phases are mixed according to the proportion of the oil phase, namely the oil phase accounts for 50-70 percent after being mixed.
The hyaluronic acid or its salt aqueous solution and the oil phase are stored in different containers and used together, and the container is not limited as long as the hyaluronic acid or its salt aqueous solution and the oil phase can be stored.
The invention is not limited in any way as far as it can serve a corresponding function, with respect to a disposable package.
The invention provides the use of triglycerides, which are side chain fully alkylated triglycerides having a viscosity of less than 85 centipoise, for improving the skin care properties of hyaluronic acid or a salt thereof.
The improvement of the skin care effect of hyaluronic acid or a salt thereof in the present invention refers to the improvement of the skin care effect, particularly the moisture retention of hyaluronic acid or a salt thereof.
The invention provides application of the composition in wet tissue liquid.
The composition can be used in wet tissue liquid to improve the skin care performance of hyaluronic acid or a salt thereof in the wet tissue.
Examples
The invention is described generally and/or specifically for the materials used in the tests and the test methods, in the following examples,% means wt%, i.e. percent by weight, unless otherwise specified. The reagents and instruments used are not indicated by manufacturers, and are all conventional reagent products commercially available, wherein the raw materials used in the examples and comparative examples are shown in Table 1.
TABLE 1 raw material tables for examples and comparative examples
| Oil and fat | Suppliers of goods |
| Castor oil | SHANGHAI MACKLIN BIOCHEMICAL Co.,Ltd. |
| Olive oil | Dahlia chemical (Shanghai) Co Ltd |
| Potentilla chinensis seed oil | Guangzhou Xianting cosmetics raw materials Co Ltd |
| Avocado oil | Vantage Specialty Chemicals |
| Mineral oil | Basf-Fr |
| Caprylic capric acid triglyceride | Basf-Fr |
| Fatty acid ethyl ester | Ashiscard oil & fat (Shanghai) Co., ltd. (Shealight oil) |
Example 1
And mixing the oil phase and the sodium hyaluronate aqueous phase solution to obtain an emulsion, wherein the components of the sodium hyaluronate aqueous phase solution are shown in table 2, the components of the oil phase are shown in table 3, and the content of the oil phase in the emulsion after the emulsion is prepared is shown in table 4, namely, the emulsion is case-18-case-26.
Comparative example 1
Mixing an optional oil phase and an optional aqueous sodium hyaluronate solution to obtain an emulsion or solution, wherein the components of the aqueous sodium hyaluronate solution are shown in table 2, the components of the oil phase are shown in table 3, and the content of the oil phase in the emulsion after the emulsion is prepared is shown in table 4, and the contents of the oil phase in the emulsion are case-1-case-16 and case-26-case-36.
TABLE 2 sodium hyaluronate aqueous phase ingredients Table
TABLE 3 oil phase ingredients Table
Wherein, the measuring method of the viscosity of the oil phase is as follows: detecting by adopting an Ametex viscometer DV-1, selecting a rotor RV-3 at the rotating speed of 30rpm, ensuring that the head of the rotor is completely immersed in a sample to be detected when measuring the viscosity, ensuring that the rotor does not contact the four walls and the bottom of a sample container, and reading a numerical value after the instrument is stabilized for 10-20 s.
TABLE 4 proportion of oil phase in the emulsion
Experimental example 1 method for measuring moisture content of skin
15 subjects were selected in this experiment, with the age between 18-65 years, except for gestation or lactation; patients without serious systemic diseases, immunodeficiency and autoimmune diseases; the subject site has not received skin treatment, cosmetic, and other tests that may affect outcome; those with inactive allergic disease; those without constitutional hypersensitivity; hormone drugs and immunosuppressants have not been used in the last month; the test site is not present in other clinical experimenters at present or in the last 3 months.
The skin moisture content test was performed on the forearm of the subject, and the entire test was performed in a room at 20-22 deg.C and 40% -60% relative humidity.
Volunteers were unable to contact water with the forearms 3 hours prior to testing, and the forearm flexor was wiped with a facial tissue prior to testing. On the left and right forearm flexor sides of the subject, 3x 3cm square test areas were marked, each area being 1cm apart. The test site is exposed to the test environment for at least 20min before testing.
Before applying the samples, the moisture content of the skin in different areas of the arm was measured using an instrument skin moisture tester CM825 (Courage + Khazaka Co., germany) and the original values were recorded.
For cases-1 to 4, the test sample was only an aqueous solution of hyaluronic acid or a salt thereof. 120 μ L of the sample was removed using a pipette, transferred directly to the test area of the subject's arm, rubbed with a finger (with rubber gloves), rotated 40 cycles, and patted dry with a paper towel.
For cases-8-16, the test samples had only the oil phase. 120 μ L of sample was removed using a pipette, transferred directly to the test area of the subject's arm, rubbed 40 cycles with a finger (with rubber gloves), and patted dry with a paper towel.
For cases-17 to 36, the test sample was a mixture of oil and water phases. Sealing the oil phase and the water phase in the same glass bottle according to a set proportion, and shaking for 1 minute to enable the oil phase and the water phase to form emulsion. And (3) within 10 seconds after the oscillation is finished, when the oil-water two phases do not obviously layer, transferring a 120 mu L sample by using a liquid transfer gun, directly transferring the sample into an arm picture frame area of a subject, rubbing the sample for 40 circles by using fingers (with rubber gloves), and lightly patting the sample dry by using a paper towel.
After 4 hours of sample application, the moisture content of the skin applied to the test area was measured using a skin moisture tester, corneometer CM 825. The moisturizing coefficient W is defined as follows:
w = (skin moisture content after sample is smeared for 4 h-skin moisture content before sample is smeared)/skin moisture content before sample is smeared
Each test sample was tested 15 times on the skin of different subjects, and the W value was calculated using the average. Statistical comparisons were made with the moisture retention coefficients of the HA water phase alone, and the oil phase alone, using 15 sets of data for each case, using a t-test method with a confidence interval of 90%.
Since the sample coating amount of each case is the same and is 120 μ L, the mixture can be judged whether to have the synergistic effect of moisture retention only by comparing the statistical difference between the W value of the oil-water mixture, the W value of the pure water phase and the W value of the pure oil phase, and the result is shown in table 5, wherein the statistical judgment of the synergistic effect of moisture retention is judged by the following method: in the known t-test method (also called student t-test method), a confidence interval is set to 90%, the W value of an oil-water mixture is statistically compared with the W value of a pure water phase and the W value of a pure oil phase by an average value, if t-test shows statistically significant difference, the result is marked as "significant", if t-test does not show significant difference, the result is marked as "insignificant", and FIG. 1 is a schematic diagram of the moisturizing coefficient of hyaluronic acid or a salt aqueous solution thereof coated with case-1.
TABLE 5 moisturizing coefficients and statistical judgment
As can be seen from table 5, the moisturizing factor was improved only under the conditions described herein, and the effect was statistically significant, while for case-1 (as shown in fig. 1), the moisturizing factor W was not statistically significantly increased relative to the baseline (W = 0).
In summary, the composition of the present invention, when formulated into an emulsion for application to a desired area of a subject, can increase the moisture content of the skin, reduce the excessive swelling of the stratum corneum, and avoid the irritation of surfactants, thereby improving the skin barrier function.
The foregoing is directed to preferred embodiments of the present invention, other and further embodiments of the invention may be devised without departing from the basic scope thereof, and the scope thereof is determined by the claims that follow. However, any simple modification, equivalent change and modification of the above embodiments according to the technical essence of the present invention are within the protection scope of the technical solution of the present invention.
Claims (15)
1. A method of improving the moisture retention of hyaluronic acid or a salt thereof, comprising:
mixing an oil phase and a hyaluronic acid or a salt water phase solution thereof to obtain an emulsion, wherein the oil phase is triglyceride with fully alkylated side chains, the viscosity of the oil phase is less than 85 centipoise, and the mass percentage of the oil phase in the emulsion is 45-75 percent,
the molecular weight of the hyaluronic acid or the salt thereof is more than or equal to 1w Da, and the mass percentage of the hyaluronic acid or the salt thereof in the emulsion is more than 0.01%.
2. The method of claim 1, wherein the oil phase is 50-70% by weight of the emulsion.
3. The method of claim 1, wherein the viscosity of the oil phase is 30-85 centipoise.
4. The method according to claim 1, wherein the oil phase is one or more selected from the group consisting of meadowfoam seed oil, olive oil, and caprylic/capric triglyceride.
5. The method according to any one of claims 1 to 4, wherein the hyaluronic acid or a salt thereof is one or more selected from the group consisting of hyaluronic acid, sodium hyaluronate, potassium hyaluronate, zinc hyaluronate, magnesium hyaluronate, calcium hyaluronate, and gold hyaluronate.
6. The method of any one of claims 1-4, wherein the hyaluronic acid salt is sodium hyaluronate.
7. The method of any one of claims 1-4, wherein the hyaluronic acid, or salt thereof, has a molecular weight of 1-200 w Da.
8. The composition for improving the moisture retention of the hyaluronic acid or the salt thereof is formed by mixing an aqueous phase solution of the hyaluronic acid or the salt thereof with an oil phase, wherein the oil phase is triglyceride with fully alkylated side chains, the viscosity of the oil phase is less than 85 centipoise, the mass percentage of the oil phase in the composition is 45-75%, the molecular weight of the hyaluronic acid or the salt thereof is more than or equal to 1w Da, and the mass percentage of the hyaluronic acid or the salt thereof in the composition is more than 0.01%.
9. The composition of claim 8, wherein the oil phase is 50-70% by weight of the composition; the viscosity of the oil phase is 30-85 centipoises;
the oil phase is one or more selected from white chinlon oil, olive oil and caprylic/capric triglyceride.
10. The composition according to claim 8, wherein the hyaluronic acid or a salt thereof is one or more selected from the group consisting of hyaluronic acid, sodium hyaluronate, potassium hyaluronate, zinc hyaluronate, magnesium hyaluronate, calcium hyaluronate, and gold hyaluronate.
11. The composition of any one of claims 8-10, wherein the hyaluronic acid salt is sodium hyaluronate.
12. The composition of any one of claims 8-10, wherein the hyaluronic acid or salt thereof has a molecular weight of 1w-200w Da.
13. A method of using the composition of any one of claims 8-12, comprising:
preparing hyaluronic acid or salt thereof into an aqueous phase solution, and then mixing the aqueous phase solution with an oil phase to obtain an emulsion;
the resulting emulsion is applied to the desired area of the subject.
14. A method of using the composition of any one of claims 8-12, comprising any one of:
(1) Storing the hyaluronic acid or its salt water phase solution and oil phase in the same container, layering the water phase and oil phase in the container, shaking before use, and pouring out or spraying out;
(2) The hyaluronic acid or salt water phase solution and the oil phase are stored in the same container, the water phase and the oil phase are stored in a partition mode at intervals, and the two phases are pumped out according to a certain proportion through a pump head when the hyaluronic acid or salt water phase solution and the oil phase solution are used;
(3) Storing the hyaluronic acid or its salt water phase solution and oil phase in different containers, and using in combination;
(4) The aqueous solution of hyaluronic acid or salt thereof and the oil phase are stored in a single-use package in the form of a disposable or ampoule.
15. Use of a composition according to any one of claims 8 to 12 in the preparation of a wet wipe liquor.
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| CN202111320857.8A CN114042013B (en) | 2021-11-09 | 2021-11-09 | Composition for improving skin care property of hyaluronic acid or salt thereof and method thereof |
| PCT/CN2021/136948 WO2023082391A1 (en) | 2021-11-09 | 2021-12-10 | Composition and method for improving skin care property of hyaluronic acid or salt thereof |
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| CN202111320857.8A CN114042013B (en) | 2021-11-09 | 2021-11-09 | Composition for improving skin care property of hyaluronic acid or salt thereof and method thereof |
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| CN110755288A (en) * | 2019-09-26 | 2020-02-07 | 广州市瑞品化妆品有限公司 | Cosmetic composition for moisturizing skin and preparation method thereof |
| CN110882174A (en) * | 2019-12-27 | 2020-03-17 | 广州环亚化妆品科技有限公司 | Moisturizing emulsion and preparation method thereof |
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| KR101003847B1 (en) * | 2010-01-18 | 2010-12-23 | 주식회사 씨앤피차앤박화장품 | Skin -like complex composition |
| JP2013006790A (en) * | 2011-06-24 | 2013-01-10 | Mikimoto Pharmaceut Co Ltd | O/w-type emulsion |
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