CN114032173A - Closed air sampling bottle - Google Patents
Closed air sampling bottle Download PDFInfo
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- CN114032173A CN114032173A CN202210023858.4A CN202210023858A CN114032173A CN 114032173 A CN114032173 A CN 114032173A CN 202210023858 A CN202210023858 A CN 202210023858A CN 114032173 A CN114032173 A CN 114032173A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/06—Magnetic means
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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Abstract
The invention discloses a closed air sampling bottle, which relates to the technical field of microbial detection and comprises a bottle body, an elution device and an electromagnet adjuster, wherein sampling liquid is stored in the bottle body; the elution device is arranged on the outer side of the bottle body and comprises an eluent storage cavity for storing eluent and a movable cavity arranged at the top of the eluent storage cavity, the eluent storage cavity is provided with a liquid outlet pipe, and the electromagnet adjuster can move in the movable cavity; an isolating membrane is arranged between the movable cavity and the inner cavity of the bottle body; by utilizing the sampling bottle, the collection of air microorganism samples can be completed, the nucleic acid extraction can be completed under the airtight condition, the obtained nucleic acid extracting solution can be directly used for detection, the mixed bacteria pollution caused by the complicated operation is avoided, the aerosol pollution caused by the nucleic acid extraction process is also avoided, the operation is simple, and the detection process of the air microorganism is accelerated. In addition, this sampling bottle can produce as disposable sampling bottle to avoid the sample pollution that reuse introduced.
Description
Technical Field
The invention relates to the technical field of microorganism detection, in particular to a closed air sampling bottle.
Background
At present, microorganisms in human, animal and plant bodies and soil can be dispersed in the air by droplets, dust, and the like, so that the air contains a certain kind and number of microorganisms. There are generally no pathogenic microorganisms in the air theoretically, but aerosols of pathogenic microorganisms are often suspended in the air near hospitals, veterinary hospitals and livestock houses, and healthy people or animals are often infected by inhalation. Air contaminated by pathogenic microorganisms can often be a source or vehicle of contamination, causing epidemic disease. Therefore, the detection of airborne microorganisms is of great significance for the prevention and control of infectious diseases and the hygiene supervision and protection of the environment.
The air sampling bottle is an important tool in the air microorganism detection process and is various, wherein the impact type air sampling bottle collects microorganism particles in the air into sampling liquid in a jet airflow mode. In the sampling process, after the sampling liquid is added into the sampling bottle, the air suction power is started, air enters from the inlet of the sampling bottle, microbial particles in the air impact the sampling liquid of the sampling bottle, and the microbial particles are captured due to the adhesion of the liquid.
Common impact type air sampling bottle simple structure, the air microorganism sample of collection generally can not directly be used for detecting, but need with air microorganism sample, sampling solution, can obtain detectable microorganism sample after carrying out operations such as extra cultivation or enrichment, nucleic acid extraction, complex operation, required instrument and equipment are many, consuming time is long, and introduce non-sampling bacterial contamination easily in follow-up operation, be unfavorable for the short-term test to air microorganism.
Disclosure of Invention
The invention aims to provide a closed air sampling bottle to solve the problems in the prior art, the sampling bottle comprises a structure required by air microorganism sample collection and nucleic acid extraction, the sampling bottle can be used for completing the collection and nucleic acid extraction of the air microorganism sample, the operation is simple, the mixed bacteria pollution caused by complicated operation is avoided, and the operation is convenient and rapid.
In order to achieve the purpose, the invention provides the following scheme:
the invention provides a closed air sampling bottle, which comprises a bottle body, an elution device and an electromagnet adjuster, wherein an air inlet pipe and an exhaust pipe are arranged on the bottle body, and sampling liquid is stored in the bottle body; the elution device is arranged on the outer side of the bottle body and comprises an eluent storage cavity for storing eluent and a movable cavity arranged at the top of the eluent storage cavity, a liquid outlet pipe is arranged in the eluent storage cavity, and an opening with a sealing cover is arranged between the top of the eluent storage cavity and the movable cavity; an isolating membrane is arranged between the movable cavity and the inner cavity of the bottle body;
the electromagnet adjuster comprises a wire box arranged outside the movable cavity and a pushing rod connected with the wire box; the utility model discloses a wire unwinding device, including propelling rod, line box, wire box, electromagnet, propelling rod, wire box, the propelling rod transversely alternates on the lateral wall in activity chamber, be provided with the coil that has the electric wire in the line box, rotate the coil can be realized the receiving and releasing of electric wire, the end-to-end connection of electric wire has the electro-magnet, make when the electric wire tightens up the electro-magnet is fixed the end of propelling rod, the electric wire unreel when unclamping the release the electro-magnet by the propelling rod end drops, the electric wire still is used for giving the electro-magnet circular telegram makes the electro-magnet produces magnetism.
Preferably, the pipe orifices of the air inlet pipe and the air exhaust pipe are provided with pipe caps for controlling the opening and closing of the pipe orifices, and the pipe orifice of the liquid outlet pipe is provided with a valve for controlling the on-off of the liquid outlet pipe.
Preferably, the bottom of pushing ram is provided with a push pedal, the inboard of the lateral wall in activity chamber is provided with solid fixed ring, the body of rod of pushing ram passes gu fixed ring by gu fixed ring leads, gu fixed ring's bottom is provided with the confession the opening that the push pedal passes through.
Preferably, the sealing cover is arranged at the bottom of the bottom plate of the movable cavity, one end, close to the bottle body, of the sealing cover is hinged to the bottom plate of the movable cavity, one end, far away from the bottle body, of the sealing cover is connected with the bottom plate of the movable cavity through a buckle, when the push plate is driven by the pushing rod to push the bottle body, the bottom end of the push plate pushes the buckle structure, and the sealing cover is opened.
Preferably, the electromagnet consists of a cylinder and a cone, the tip of the cone is arranged towards the isolating membrane, the bottom surface of the cone is connected with the cylinder, one end of the wire is wound on the coil, the other end of the wire is wound on the cylinder part of the electromagnet, and the cylinder of the electromagnet and the wire wound outside the cylinder of the electromagnet are embedded in a fixed block.
Preferably, the fixed block is cylindrical, the diameter of the fixed block is smaller than that of the push rod, and the diameter of the fixed block is larger than that of the cylindrical passage in the push rod.
Preferably, an electric outlet for energizing the electric wire is provided on an outer side of the wire cassette.
Preferably, the outside opposite side of line box is provided with the pivot that is used for rotating coil, one side of pivot is provided with fixed knot constructs, wear to be equipped with the fixed rod on the fixed knot structure, the fixed rod can push up the pivot makes the unable free rotation of pivot.
Preferably, the elution device is detachably connected with the bottle body.
Compared with the prior art, the invention has the following beneficial technical effects:
the invention provides a closed air sampling bottle, which comprises a structure required by air microorganism sample collection and nucleic acid extraction, wherein different cavities of the sampling bottle are stored with cracking binding solution and eluent, the cracking binding solution is used as the sampling solution to collect air microorganisms, and the air microorganisms can be cracked while the air microorganism samples are collected. By utilizing the sampling bottle, the collection of air microorganism samples can be completed, the nucleic acid extraction can be completed under the airtight condition, the obtained nucleic acid extracting solution can be directly used for detection, the mixed bacteria pollution caused by the complicated operation is avoided, the aerosol pollution caused by the nucleic acid extraction process is also avoided, the operation is simple, and the detection process of the air microorganism is accelerated. In addition, this sampling bottle can produce as disposable sampling bottle to avoid the sample pollution that reuse introduced.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without creative efforts.
FIG. 1 is a schematic perspective view of a sealed air sampling bottle according to the present invention;
FIG. 2 is a schematic view of the elution apparatus of the present invention;
FIG. 3 is a schematic diagram of the electromagnet adjuster of the present invention;
FIG. 4 is a schematic view of the electromagnet and the fixing block according to the present invention;
FIG. 5 is a schematic perspective view of an electromagnet actuator according to the present invention;
FIG. 6 is a schematic view of the working state of the sealed air sampling bottle of the present invention, specifically, the state in which the electromagnet punctures the isolation film and enters the inner cavity of the bottle body;
FIG. 7 is a schematic view of the working state of the sealed air sampling bottle of the present invention, specifically the state of the electromagnet falling into the bottle body;
FIG. 8 is a schematic view of the working state of the sealed air sampling bottle of the present invention, specifically the state of the electromagnet entering the eluent storage chamber;
in the figure: 1-bottle body, 101-air inlet pipe, 102-air exhaust pipe;
2-an elution device, 201-an eluent storage cavity, 202-a movable cavity, 203-a sealing cover, 204-an isolating membrane, 205-a buckle and 206-a liquid outlet pipe;
3-electromagnet adjuster, 301-wire box, 302-coil, 303-plug port, 304-rotating shaft, 305-fixed structure, 306-fixed rod, 307-fixed block, 308-pushing rod, 309-electromagnet, 310-wire, 311-pushing plate and 312-fixed ring.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The invention aims to provide a closed air sampling bottle to solve the problems in the prior art.
In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in further detail below.
The closed air sampling bottle in this embodiment, as shown in fig. 1-5, includes a bottle body 1, an elution device 2 and an electromagnet adjuster 3, the bottle body 1 is provided with an air inlet pipe 101 and an air exhaust pipe 102, and the bottle body 1 stores a sampling solution; the elution device 2 is arranged on the outer side of the bottle body 1, the elution device 2 comprises an eluent storage cavity 201 for storing eluent and a movable cavity 202 arranged at the top of the eluent storage cavity 201, the eluent storage cavity 201 is provided with a liquid outlet pipe 206, eluent can flow out of the liquid outlet pipe 206, an opening with a sealing cover 203 is arranged between the top of the eluent storage cavity 201 and the movable cavity 202, and the arrangement of the sealing cover 203 can prevent the leakage of the eluent in the eluent storage cavity 201 in the transportation process of a sampling bottle; an isolating membrane 204 is arranged between the movable cavity 202 and the inner cavity of the bottle body 1, and the isolating membrane 204 can prevent the sampling liquid in the bottle body 1 from entering the movable cavity 202;
the electromagnet adjuster 3 includes a wire box 301 disposed outside the movable chamber 202 and a push rod 308 connected to the wire box 301; the push rod 308 is transversely inserted on the outer side wall of the movable cavity 202 in a penetrating mode, a coil 302 with an electric wire 310 is arranged in the wire box 301, the coil 302 can be rotated to achieve winding and unwinding of the electric wire 310, the tail end of the electric wire 310 is connected with the electromagnet 309, the electromagnet 309 is fixed at the tail end of the push rod 308 when the electric wire 310 is tightened, the electromagnet 309 falls from the tail end of the push rod 308 when the electric wire 310 is unwound and loosened, and the electric wire 310 is further used for electrifying the electromagnet 309 to enable the electromagnet 309 to generate magnetism.
In this embodiment, the openings of the air inlet pipe 101 and the air exhaust pipe 102 are provided with pipe caps for controlling the opening and closing of the openings, and the opening of the liquid outlet pipe 206 is provided with a valve for controlling the on-off of the liquid outlet pipe 206.
The bottom of pushing ram 308 is provided with a push pedal 311, the inboard of the lateral wall in activity chamber 202 is provided with solid fixed ring 312, the body of rod of pushing ram 308 passes solid fixed ring 312 and is led by solid fixed ring 312, the bottom of solid fixed ring 312 is provided with the opening that supplies push pedal 311 to pass through, sealed lid 203 sets up in the bottom of the bottom plate in activity chamber 202, the one end that sealed lid 203 is close to bottle 1 is articulated with the bottom plate in activity chamber 202, sealed lid 203 is kept away from and is connected through buckle 205 between the bottom plate of bottle 1's the one end and activity chamber 202, push pedal 311 is when pushing away to bottle 1 under pushing ram 308's the drive, the bottom of push pedal 311 promotes buckle 205 structure, make sealed lid 203 open, and then the opening between eluent storage chamber 201 and the activity chamber 202 is opened.
In this embodiment, the electromagnet 309 is composed of a cylinder and a cone, the tip of the cone is disposed toward the isolation film 204, the bottom surface of the cone is connected to the cylinder, one end of the wire 310 is wound on the coil 302, the other end of the wire 310 is wound on the cylinder portion of the electromagnet 309, and the cylinder of the electromagnet 309 and the wire 310 wound outside thereof are embedded in a fixed block 307; wherein, the fixed block 307 is cylindrical, the diameter of the fixed block 307 is smaller than the diameter of the push rod 308, and the diameter of the fixed block 307 is larger than the inner diameter of the cylindrical passage of the push rod 308, so that when the coil 302 retracts the wire 310, the fixed block 307 and the electromagnet 309 are finally fixed outside the push rod 308 and do not enter the push rod 308; the fixed block 307 is made of a nonmagnetic material, so that when the electromagnet 309 is energized, magnetic beads are attracted only to the side surface of the conical portion of the electromagnet 309.
In this embodiment, the external side of the wire box 301 is provided with a plug-in port 303 for energizing the electric wire 310; the outside opposite side of line box 301 is provided with pivot 304 that is used for rotating coil 302, and one side of pivot 304 is provided with fixed knot 305, wears to be equipped with fixed rod 306 on the fixed knot 305, and fixed rod 306 can push up pivot 304 and make pivot 304 unable free rotation to prevent that the terminal electro-magnet 309 of electric wire 310 from free fall under the action of gravity, play indirect fixed action to electro-magnet 309.
In this embodiment, the elution device 2 is detachably connected to the bottle, and since the electromagnet adjuster 3 is installed on the elution device 2, the cost is relatively high, after the sampling bottle is used up each time, the elution device 2 can be detached from the bottle for washing, and a new bottle is used repeatedly next time, so that the cost is saved.
The working process of the closed air sampling bottle disclosed by the invention is as follows:
the sampling bottle is closed, before use, the opening of the air inlet pipe 101 and the opening of the air exhaust pipe 102 are sealed through pipe caps, and the opening of the liquid outlet pipe is sealed through a valve (not shown in the figure).
1. And opening a pipe cover of a port of the air inlet pipe 101 and a port of the air pumping pipe 102, adding magnetic beads into the sampling bottle from the port of the air pumping pipe 102 of the closed air sampling bottle, and connecting the air pumping pipe 102 with a vacuum pump to perform air sampling.
2. During sampling, the air microorganisms are cracked under the action of the sampling liquid (cracking binding liquid), and nucleic acid is released to the sampling liquid and adsorbed onto the magnetic beads.
3. After sampling, the opening of the air inlet pipe 101 and the opening of the exhaust pipe 102 are sealed by a pipe cover.
4. The pushing rod 308 of the electromagnet adjuster 3 is pushed to make the electromagnet 309 puncture the isolation film 204 and enter the inner cavity of the vial 1, and at the same time, the push plate 311 opens the sealing cover 203 of the eluent storage chamber 201 (as shown in fig. 6).
5. The spindle 304 of the electromagnet adjuster 3 is rotated to release the electromagnet 309, so that the electromagnet 309 falls into the sample solution in the vial 1 (as shown in fig. 7).
6. The electromagnet 309 is energized, and the magnetic beads in the sampling solution are adsorbed to the electromagnet 309.
7. The rotating shaft 304 on the electromagnet adjuster 3 is rotated to withdraw the electromagnet 309 and move the pushing rod 308 back at the same time until the pushing rod 308 cannot move back (when the pushing plate 311 moves back to the right side wall of the movable cavity 202, the pushing plate 311 cannot move back continuously under the block of the right side wall, and then the pushing rod 308 cannot move back continuously), at this time, the electromagnet 309 is just above the opening of the eluent storage cavity 201.
8. Rotating the shaft 304 of the electromagnet actuator 3 releases the electromagnet 309, allowing the electromagnet 309 to enter the eluant (as shown in figure 8).
9. The electromagnet 309 is powered off, the magnetic beads on the electromagnet 309 are released into the eluent, and the nucleic acids on the magnetic beads are eluted into the eluent (the electromagnet can be repeatedly withdrawn and released to impact and stir the eluent, so that the nucleic acids on the magnetic beads are more fully eluted into the eluent).
10. The electromagnet 309 is energized to adsorb the magnetic beads having eluted nucleic acids to the electromagnet 309.
11. The nucleic acid sample of the air microorganism can be taken out by opening the valve of the liquid outlet pipe orifice.
The principle and the implementation mode of the invention are explained by applying specific examples, and the description of the above examples is only used for helping understanding the method and the core idea of the invention; meanwhile, for a person skilled in the art, according to the idea of the present invention, the specific embodiments and the application range may be changed. In summary, this summary should not be construed to limit the present invention.
Claims (9)
1. A closed air sampling bottle which is characterized in that: the device comprises a bottle body, an elution device and an electromagnet adjuster, wherein an air inlet pipe and an exhaust pipe are arranged on the bottle body, and sampling liquid is stored in the bottle body; the elution device is arranged on the outer side of the bottle body and comprises an eluent storage cavity for storing eluent and a movable cavity arranged at the top of the eluent storage cavity, a liquid outlet pipe is arranged in the eluent storage cavity, and an opening with a sealing cover is arranged between the top of the eluent storage cavity and the movable cavity; an isolating membrane is arranged between the movable cavity and the inner cavity of the bottle body;
the electromagnet adjuster comprises a wire box arranged outside the movable cavity and a pushing rod connected with the wire box; the utility model discloses a wire winding machine, including propelling rod, line box, electromagnet, propelling rod, wire box, the propelling rod transversely alternates on the lateral wall in activity chamber, be provided with the coil that has the electric wire in the line box, rotate the coil can be realized the receiving and releasing of electric wire, the end-to-end connection of electric wire has the electro-magnet, make when the electric wire tightens up the electro-magnet is fixed the end of propelling rod, when the electric wire unreels and loosens the electro-magnet by the propelling rod end drops, the electric wire still is used for the electro-magnet circular telegram makes the electro-magnet produces magnetism.
2. The hermetic air sampling bottle according to claim 1, wherein: the pipe orifice of intake pipe with the exhaust tube is provided with the tube cap of control mouth of pipe switch, the mouth of pipe department of drain pipe is provided with the control the valve of drain pipe break-make.
3. The hermetic air sampling bottle according to claim 1, wherein: the bottom of pushing ram is provided with a push pedal, the inboard of the lateral wall in activity chamber is provided with solid fixed ring, the body of rod of pushing ram passes gu fixed ring by gu fixed ring direction, gu fixed ring's bottom is provided with the confession the opening that the push pedal passes through.
4. The hermetic air sampling bottle according to claim 1, wherein: the sealing cover is arranged at the bottom of the bottom plate of the movable cavity, one end, close to the bottle body, of the sealing cover is hinged to the bottom plate of the movable cavity, one end, far away from the bottle body, of the sealing cover is connected with the bottom plate of the movable cavity through a buckle, the push plate is driven by the push rod to move downwards, when the bottle body is pushed, the bottom end of the push plate pushes the buckle structure, and the sealing cover is opened.
5. The hermetic air sampling bottle according to claim 1, wherein: the electromagnet is composed of a cylinder and a cone, the tip end of the cone faces the isolating membrane, the bottom surface of the cone is connected with the cylinder, one end of the electric wire is wound on the coil, the other end of the electric wire is wound on the cylinder portion of the electromagnet, and the cylinder of the electromagnet and the electric wire wound outside the cylinder of the electromagnet are embedded in a fixed block in a embedding mode.
6. The hermetic air sampling bottle according to claim 5, wherein: the fixed block is cylindrical, the diameter of the fixed block is smaller than that of the push rod, and the diameter of the fixed block is larger than that of a cylindrical channel in the push rod.
7. The hermetic air sampling bottle according to claim 1, wherein: and an electric plug port for electrifying the electric wire is arranged on one side of the outside of the wire box.
8. The hermetic air sampling bottle according to claim 7, wherein: the outside opposite side of line box is provided with and is used for rotating the pivot of coil, one side of pivot is provided with fixed knot constructs, wear to be equipped with the fixed rod on the fixed knot structure, the fixed rod can push up the pivot makes the unable free rotation of pivot.
9. The hermetic air sampling bottle according to claim 1, wherein: the elution device is detachably connected with the bottle body.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202210023858.4A CN114032173B (en) | 2022-01-11 | 2022-01-11 | Closed air sampling bottle |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202210023858.4A CN114032173B (en) | 2022-01-11 | 2022-01-11 | Closed air sampling bottle |
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| CN114032173A true CN114032173A (en) | 2022-02-11 |
| CN114032173B CN114032173B (en) | 2022-03-15 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114540177A (en) * | 2022-04-25 | 2022-05-27 | 至美时代生物智能科技(北京)有限公司 | Impact type air sampler |
| CN114774265A (en) * | 2022-06-22 | 2022-07-22 | 至美时代生物智能科技(北京)有限公司 | Automatic air microorganism detection system |
| CN115141739A (en) * | 2022-09-06 | 2022-10-04 | 至美时代生物智能科技(北京)有限公司 | Air microorganism sampling and detection integrated device and sampling and detection method |
| CN117244601A (en) * | 2023-11-20 | 2023-12-19 | 至美时代生物智能科技(北京)有限公司 | Liquid quantitative device |
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