CN114026989A - Method for breaking dormancy of wild anemone seed - Google Patents
Method for breaking dormancy of wild anemone seed Download PDFInfo
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- CN114026989A CN114026989A CN202111315203.6A CN202111315203A CN114026989A CN 114026989 A CN114026989 A CN 114026989A CN 202111315203 A CN202111315203 A CN 202111315203A CN 114026989 A CN114026989 A CN 114026989A
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- 241001083548 Anemone Species 0.000 title claims abstract description 65
- 238000000034 method Methods 0.000 title claims abstract description 23
- 230000005059 dormancy Effects 0.000 title claims abstract description 18
- 239000004576 sand Substances 0.000 claims abstract description 45
- 230000001954 sterilising effect Effects 0.000 claims abstract description 42
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 38
- 238000002791 soaking Methods 0.000 claims abstract description 24
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 24
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 16
- 238000005406 washing Methods 0.000 claims abstract description 7
- 238000002156 mixing Methods 0.000 claims abstract description 6
- 238000001816 cooling Methods 0.000 claims abstract description 5
- 238000001035 drying Methods 0.000 claims abstract description 5
- 238000007873 sieving Methods 0.000 claims abstract description 5
- 238000004806 packaging method and process Methods 0.000 claims abstract description 3
- 238000012258 culturing Methods 0.000 claims abstract 2
- 241000600332 Anemone vitifolia Species 0.000 claims description 2
- 230000035784 germination Effects 0.000 abstract description 16
- 241000196324 Embryophyta Species 0.000 abstract description 6
- 239000012153 distilled water Substances 0.000 description 10
- 230000009286 beneficial effect Effects 0.000 description 7
- 230000000052 comparative effect Effects 0.000 description 6
- 241000894006 Bacteria Species 0.000 description 2
- 241000234587 Canna Species 0.000 description 2
- 235000005273 Canna coccinea Nutrition 0.000 description 2
- 241000628997 Flos Species 0.000 description 2
- 241000233866 Fungi Species 0.000 description 2
- 241000219098 Parthenocissus Species 0.000 description 2
- 241000218201 Ranunculaceae Species 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 244000052616 bacterial pathogen Species 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 238000005057 refrigeration Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 238000013517 stratification Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 238000009736 wetting Methods 0.000 description 2
- 241001149941 Agelena Species 0.000 description 1
- 241000410490 Anemone canadensis Species 0.000 description 1
- 241000415044 Anemone narcissiflora Species 0.000 description 1
- 241000271042 Gloydius halys Species 0.000 description 1
- 235000006662 Lansium Nutrition 0.000 description 1
- 241001156382 Lansium Species 0.000 description 1
- 241001530121 Trollius Species 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 235000017807 phytochemicals Nutrition 0.000 description 1
- 229930000223 plant secondary metabolite Natural products 0.000 description 1
- 230000014284 seed dormancy process Effects 0.000 description 1
- 230000007226 seed germination Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/06—Coating or dressing seed
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Soil Sciences (AREA)
- Environmental Sciences (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention discloses a method for breaking dormancy of wild anemone seed, which comprises the following steps: (1) soaking wild anemone seeds in clear water, soaking in alcohol, and soaking with H2O2Sterilizing, and finally washing with water; (2) drying sand, sieving, sterilizing at high temperature and high pressure, naturally cooling to room temperature, moistening with water, mixing with sterilized seeds, and packaging into perforated sterilized bags; (3) paving a first layer of sand on the ground, putting the sterilization bags in order, adding a second layer of sand, cutting the second layer of sand to be flush with the sand surface to form an open upper opening, and covering a third layer of sand; (4) placing the sterilization bag in a cellar for 90-100 days, transferring to room temperature, standing for 10-15 days, and culturing to obtain wild anemone seedlings. The method greatly promotes the anemone rhizomesThe germination of the flowers and the seeds of the anemone, the treatment condition is simpler, the realization is convenient, and the technical support is provided for protecting and developing plant resources.
Description
Technical Field
The invention relates to the technical field of plant cultivation, in particular to a method for breaking dormancy of wild anemone seeds.
Background
Anemone canna flower (Anemone narcissiflora var. crinita (Juz.) Tamura) and Anemone canna flower (Anemone cathayensis Kitag.) are wild ornamental plants of Anemone in the genus of Anemone of Ranunculaceae, and have high medicinal value. But is limited by natural environmental conditions and artificial conditions, and the wild resources of the anemone crassipes and the anemone crassipes are very limited.
At present, the research on the anemone longissima is blank, the research on the anemone is mostly carried out on the phytochemical components and the pharmacology of the anemone, and no report is found on how to break the seed dormancy of the anemone. The difficult germination is a bottleneck problem which restricts the large-scale cultivation of the anemone creeper and the anemone, and the propagation speed is slow depending on the rhizome, so that the medicinal production cannot be rapidly met.
Therefore, the search for suitable germination conditions is a fundamental way for solving the shortage of wild anemone germplasm resources.
Disclosure of Invention
In view of the above, the present invention aims to provide a method for breaking dormancy of wild anemone seeds, so as to solve the defects in the prior art.
In order to achieve the purpose, the invention adopts the following technical scheme:
a method for breaking dormancy of wild anemone seeds specifically comprises the following steps:
(1) soaking wild anemone seeds in water, and soakingSoaking in ethanol, and adding H2O2Sterilizing, and washing with water to obtain sterilized seeds for later use;
(2) drying sand, sieving, sterilizing at high temperature and high pressure, naturally cooling to room temperature, moistening with water, mixing with sterilized seeds, and packaging into sterilized bags;
(3) paving a first layer of sand on the ground surface in the cellar, then placing the sterilization bags in order, adding a second layer of sand on the upper parts of the sterilization bags, cutting the sterilization bags to be in an open shape with the sand surface level, and finally covering a third layer of sand on the upper layers of all the sterilization bags;
(4) and (3) placing the sterilization bag in a cellar for 90-100 days, then turning to room temperature and continuing to place for 10-15 days, then taking out the sterilization bag, and picking out germinated seeds for cultivation to obtain the wild anemone seedlings.
Further, in the step (1), the wild anemone is a long-hair anemone and/or anemone.
The beneficial effect of adopting the further technical proposal is that the anemone creeper and the anemone globeflower are both wild ornamental plants of anemone of Ranunculaceae, and the medicinal value is quite high.
Further, in the step (1), the soaking temperature in water is 25 ℃ and the soaking time is 48 hours.
The method has the advantages that the germination time of the seeds can be obviously shortened by soaking the wild anemone seeds in water (seed soaking); and warm water is used to kill pathogens inside and outside the seeds by utilizing the characteristic that the heat resistance of the seeds is higher than that of pathogenic bacteria, so as to prevent and treat seedling diseases.
Further, in the step (1), the mass fraction of the alcohol is 70%, and the time for soaking in the alcohol is 30 s.
The method has the beneficial effects that the germination rate of the seeds can be obviously improved by soaking the wild anemone seeds in the alcohol. Because the alcohol can play a role in sterilization, some viruses and bacteria of the seeds can be killed when the seeds are soaked, a temporary disinfection effect can be achieved, the germination of the seeds is facilitated, and the germination rate can be improved.
Further, in the above step (1), H2O2Is 15% by mass, H2O2The time for sterilization is 20 min.
The beneficial effect of adopting the further technical scheme is that wild anemone seeds are treated with H2O2Sterilizing, not only eliminating pathogenic bacteria on seeds, but also soaking in H2O2The seed germination speed is faster, the seeds can grow healthier, and seedlings are not easy to fall down. Because of H2O2Oxygen is released during decomposition, and the requirement on oxygen content is higher during germination of seeds, so that H is properly used2O2The germination rate can also be improved by soaking the seeds in the solution.
Further, in the step (2), the temperature of the high-temperature and high-pressure sterilization is 121 ℃ and the time is 20min or 135 ℃ and the time is 40 min.
The sand sterilizing device has the beneficial effects that the sand is sterilized at high temperature and high pressure, so that common microorganisms such as bacteria and fungi can be killed, and spores can be killed.
Further, in the step (2), the volume ratio of the sand to the sterilized seeds is 3: 1.
Adopt above-mentioned further technical scheme's beneficial effect to lie in, through mixing sand and disinfection seed, can make the seed grain totally wrapped up by wet sand, increase seed coat permeability, accelerate seed inside metabolism, accelerate the seed to accomplish physiological after-ripening, promote to germinate.
Further, in the step (3), the thickness of the first layer of sand is 10-15cm, preferably 10 cm; the thickness of the second layer of sand is 5-8cm, preferably 5 cm; the thickness of the third layer of sand is 10-15cm, preferably 10 cm.
The further technical scheme has the beneficial effects that the first layer of ground sand can keep the temperature and the humidity of the bottom of the sterilization bag consistent with the ambient environment; sand is coated on the upper side of the second layer of open bags, so that water evaporation in the bags can be reduced, and the seed grains are ensured to be intact and not damaged when the fungus bags are taken; the third layer, which comprises the sand covered all around, can ensure that the environment around all the sterilization bags is consistent.
According to the technical scheme, compared with the prior art, the invention has the following beneficial effects:
1. according to the invention, through cellar stratification, the optimum germination conditions of the anemone longituba and the anemone seeds are found to be 100d + 15d at room temperature in cellar stratification, and the germination rates are respectively 34.44% and 45.78%.
2. The method greatly promotes the germination of the anemone longituba and the anemone seeds, has simpler treatment conditions, is convenient to realize, and provides technical support for protecting and developing plant resources.
Detailed Description
The technical solutions in the embodiments of the present invention are clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the following examples, plump, complete and consistent-color seeds of anemone longipedunculata and anemone canadensis are respectively selected, naturally dried and stored in a refrigerator at 4 ℃ for later use. Wherein, the anemone majus seeds are collected from mechanical forest farms in the Shihanba, and the anemone majus seeds are collected from natural protection areas in the Molingshan.
Example 1
The method for breaking dormancy of wild anemone seed specifically comprises the following steps:
(1) soaking 1000 seed of Agelena lansium in distilled water of 25 deg.C for 48 hr at the beginning of 1 month in 2021 year, soaking in 70% ethanol for 30s, and soaking in 15% H2O2Sterilizing for 20min, and washing with distilled water to obtain sterilized seeds;
(2) drying sand, sieving to remove stones inside the sand, then sterilizing at the high temperature of 121 ℃ for 20min under high pressure, naturally cooling to room temperature, adding distilled water for wetting, properly stirring and uniformly mixing the sand and the sterilized seeds in a volume ratio of 3:1, and finally subpackaging in perforated sterilized bags and marking labels for later use;
(3) covering a first layer of sand with the thickness of 10cm on the ground surface in the cellar, then respectively putting the sterilization bags in order to ensure that the same distance is kept between every two bags, adding a second layer of sand with the thickness of 5cm on the upper part of each sterilization bag, cutting the upper opening of each sterilization bag to be open, covering a third layer of sand with the thickness of 10cm on the upper layers of all the sterilization bags, and keeping the periphery of each sterilization bag to be full of the sand;
(4) and (3) placing the sterilization bag in a cellar for 100 days, then turning to room temperature and continuing to place for 15 days, then taking out the sterilization bag, and picking out germinated seeds for cultivation to obtain the anemone longituba seedlings.
Example 2
The method for breaking dormancy of wild anemone seed specifically comprises the following steps:
(1) soaking 1000 seed of flos Trollii in distilled water of 25 deg.C for 48 hr at 1 month early 2021, soaking in 70% ethanol for 30s, and soaking in 15% H2O2Sterilizing for 20min, and washing with distilled water to obtain sterilized seeds;
(2) drying sand, sieving to remove stones inside the sand, then sterilizing at high temperature and high pressure at 135 ℃ for 40min, naturally cooling to room temperature, adding distilled water for wetting, properly stirring and uniformly mixing the sand and the sterilized seeds in a volume ratio of 3:1, and finally subpackaging in perforated sterilized bags and marking labels for later use;
(3) covering a first layer of sand with the thickness of 15cm on the ground surface in the cellar, then respectively putting the sterilization bags in order to ensure that the same distance is kept between every two bags, adding a second layer of sand with the thickness of 8cm on the upper part of each sterilization bag, cutting the upper opening of each sterilization bag to be open, covering a third layer of sand with the thickness of 15cm on the upper layers of all the sterilization bags, and keeping the periphery of each sterilization bag to be full of the sand;
(4) and (3) placing the sterilization bag in a cellar for 100 days, then turning to room temperature and continuing to place for 15 days, then taking out the sterilization bag, picking out seeds for germination and cultivation, and obtaining the anemone vitifolia seedlings.
Comparative example 1
The only difference from example 1 is that the bag is removed after being placed in the cellar for 100 days.
Comparative example 2
The only difference from example 2 is that the bag is removed after being placed in the cellar for 100 days.
Comparative example 3
The difference from the example 1 is that the anemone longissima seeds are placed in a constant temperature refrigerator at 4 ℃ for refrigeration for 100 days, then are soaked in distilled water at 25 ℃ for 48 hours, then are soaked in alcohol with the mass fraction of 70% for 30 seconds, and then are soaked in H with the mass fraction of 15%2O2Sterilizing for 20min, washing with distilled water to obtain sterilized seeds, planting in seedling culture medium (imported turf), and maintaining in greenhouse to obtain young seedling of Agkistrodon Halys.
Comparative example 4
The difference from the example 2 is that the anemone seeds are refrigerated in a constant temperature refrigerator at 4 ℃ for 100 days, then are soaked in distilled water at 25 ℃ for 48 hours, then are soaked in alcohol with the mass fraction of 70% for 30 seconds, and then are soaked in H with the mass fraction of 15%2O2Sterilizing for 20min, washing with distilled water to obtain sterilized seeds, planting in seedling culture medium (imported turf), and maintaining in greenhouse to obtain young seedling of flos Trollii.
Performance testing
The treated seeds of examples 1-2 and comparative examples 1-4 were each placed, poured onto a clean table and the germinated seeds were picked with tweezers and recorded. The results are shown in Table 1.
TABLE 1 germination rates of seeds obtained in examples 1-2 and comparative examples 1-2
As can be seen from Table 1, the germination rates of the anemone longituba seeds and the anemone hybrida seeds can be remarkably improved by 100d + 15d at room temperature in the cellar compared with 100d in the cellar and 100d in the constant-temperature refrigeration at 4 ℃.
The tests show that the method greatly promotes the germination of the anemone longituba and the anemone seeds, has simpler treatment conditions, is convenient to realize, and provides technical support for protecting and developing plant resources.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (10)
1. A method for breaking dormancy of wild anemone seeds is characterized by comprising the following steps:
(1) soaking wild anemone seeds in water, soaking in alcohol, and soaking in H2O2Sterilizing, and washing with water to obtain sterilized seeds for later use;
(2) drying sand, sieving, sterilizing at high temperature and high pressure, naturally cooling to room temperature, moistening with water, mixing with sterilized seeds, and packaging into sterilized bags;
(3) covering a first layer of sand on the ground surface in the cellar, putting the sterilization bags in order, adding a second layer of sand on the upper parts of the sterilization bags, cutting the sterilization bags to be open at the upper openings of the sterilization bags flush with the sand surface, and covering a third layer of sand on the upper layers of all the sterilization bags;
(4) and (3) placing the sterilization bag in a cellar for 90-100 days, then turning to room temperature and continuing to place for 10-15 days, then taking out the sterilization bag, picking out germinated seeds, and culturing to obtain the wild anemone seedlings.
2. The method for breaking dormancy of wild anemone seeds according to claim 1, wherein in the step (1), the wild anemone is anemone longipedunculata and/or anemone hybrida.
3. The method for breaking dormancy of wild anemone speciosa seeds as claimed in claim 1, wherein in step (1), the soaking temperature in water is 25 ℃ and the soaking time is 48 h.
4. The method for breaking dormancy of wild anemone speciosa seeds as claimed in claim 1, wherein in step (1), the mass fraction of the alcohol is 70%, and the soaking time in the alcohol is 30 s.
5. The method for breaking dormancy of wild anemone speciosa seeds as claimed in claim 1, wherein in step (1), H is2O2Is 15%, said H2O2The time for sterilization is 20 min.
6. The method for breaking dormancy of wild anemone speciosa seeds as claimed in claim 1, wherein in the step (2), the temperature of the high-temperature high-pressure sterilization is 121 ℃ and the time is 20 min; or the temperature is 135 deg.C, and the time is 40 min.
7. The method for breaking dormancy of wild anemone vitifolia seeds according to claim 1, wherein in the step (2), the volume ratio of the sand to the sterilized seeds is 3: 1.
8. The method for breaking dormancy of wild anemone speciosa seeds as claimed in claim 1, wherein in step (3), the thickness of the first layer of sand is 10-15 cm.
9. The method for breaking dormancy of wild anemone speciosa seeds as claimed in claim 1, wherein in step (3), the thickness of the second layer of sand is 5-8 cm.
10. The method for breaking dormancy of wild anemone speciosa seeds as claimed in claim 1, wherein in step (3), the thickness of the third layer of sand is 10-15 cm.
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| CN202111315203.6A CN114026989B (en) | 2021-11-08 | 2021-11-08 | Method for breaking dormancy of wild anemone seed |
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| CN202111315203.6A CN114026989B (en) | 2021-11-08 | 2021-11-08 | Method for breaking dormancy of wild anemone seed |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| HU9202786D0 (en) * | 1992-08-29 | 1992-12-28 | Andras Toth | Sowing method for pulsatilla |
| CN102090179A (en) * | 2009-12-15 | 2011-06-15 | 四川省中医药科学院 | Method for processing aconitum seeds |
| CN102598917A (en) * | 2012-03-30 | 2012-07-25 | 常熟市新靓文辅亚农艺发展有限公司 | Cultivation method for breaking through woodbine seed dormancy and improving survival rate of seedlings |
-
2021
- 2021-11-08 CN CN202111315203.6A patent/CN114026989B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| HU9202786D0 (en) * | 1992-08-29 | 1992-12-28 | Andras Toth | Sowing method for pulsatilla |
| CN102090179A (en) * | 2009-12-15 | 2011-06-15 | 四川省中医药科学院 | Method for processing aconitum seeds |
| CN102598917A (en) * | 2012-03-30 | 2012-07-25 | 常熟市新靓文辅亚农艺发展有限公司 | Cultivation method for breaking through woodbine seed dormancy and improving survival rate of seedlings |
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| Title |
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| 丁万隆等: "贮藏方法对打破金莲花种子休眠的影响", 《中国中药杂志》 * |
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