CN114015570B

CN114015570B - Pipe valve frame for preparing biological product in cell factory and microscopic observation system

Info

Publication number: CN114015570B
Application number: CN202111313455.5A
Authority: CN
Inventors: 张夫坤; 袁琳; 刘昶; 姜雪鸥; 范丽丽; 杨旭全; 刘阳; 顾建阳
Original assignee: Changchun Keygen Biological Products Co Ltd
Current assignee: Changchun Keygen Biological Products Co Ltd
Priority date: 2021-11-08
Filing date: 2021-11-08
Publication date: 2022-06-21
Anticipated expiration: 2041-11-08
Also published as: CN114015570A

Abstract

The invention provides a tube valve frame and a microscopic observation system for preparing biological products in a cell factory, which comprise: the pipe valve pressing plate assembly is arranged on the stainless steel frame and communicated with a cell factory pipeline, the pipe valve pressing plate assembly is used for controlling liquid inlet and outlet of the cell factory, the stainless steel frame is arranged on the transfer trolley, and a microscopic observation system is arranged in the stainless steel frame. The invention aims to provide a tube valve frame which can solve the problems of high labor intensity and large liquid disturbance during manual operation of liquid feeding and discharging in a cell factory.

Description

Pipe valve frame for preparing biological product in cell factory and microscopic observation system

Technical Field

The invention relates to the technical field of biological product preparation, in particular to a tube valve frame and a microscopic observation system for preparing a biological product in a cell factory.

Background

As a cell culture device, a cell factory can increase the culture area in a limited space and improve the culture efficiency, and is widely used in the life science fields of vaccines, monoclonal antibodies, cell extracts and the like at present. The cell factory is mainly applied to adherent cell culture and can also be used for suspension cell culture. The cell factory has various layers and specifications, meets the requirements of laboratories and batch production, does not change the dynamic conditions of cell growth when amplified from the laboratory scale, and is simple and easy to operate.

At present, use cell factory to carry out biological product preparation, generally adopt artifical mode or traditional cell factory tipping arrangement, operating personnel need rely on visually to judge into, the discharge capacity, and manual intubate, tube drawing and control peristaltic pump pipe valve open and stop, there are the pipeline disconnection in the operation process, the liquid ration is inaccurate, the operating efficiency is low, mix the liquid and neutralize, numerous inconveniences such as liquid are deposited to the pipeline, and traditional cell factory observation system also need the manual work to carry the cell factory one by one to the microscopic observation platform, there are intensity of labour big, the liquid disturbance, defects such as observation efficiency is low. With the great application of the sterile six-axis robot in the field of cell culture, an operation scheme with high automation degree is urgently needed to realize quantitative liquid feeding and discharging and efficient microscopic observation of a cell factory, so that the manual participation degree is reduced, the risk is reduced, and the consistency of operation is ensured.

Disclosure of Invention

The invention provides a pipe valve frame for preparing biological products in a cell factory and a microscopic observation system, which are used for solving the problems of high labor intensity and large liquid disturbance during manual operation of liquid feeding and discharging in the cell factory.

Therefore, the technical scheme adopted by the invention is that the tube valve frame and the microscopic observation system for preparing the biological product in the cell factory comprise: the pipe valve pressing plate assembly is arranged on the stainless steel frame and communicated with a cell factory pipeline, the pipe valve pressing plate assembly is used for controlling liquid inlet and outlet of the cell factory, and the stainless steel frame is arranged on the transfer trolley.

Preferably, the pipe valve pressing plate assembly comprises a pressing plate box, the pressing plate box is clamped with the stainless steel frame, six peristaltic pump pipe valves are fixedly arranged on the pressing plate box, four of the peristaltic pump pipe valves are respectively used for controlling the opening and closing of the cell factory pipeline, and the other two peristaltic pump pipe valves are respectively used for controlling the opening and closing of the main liquid inlet pipeline and the main liquid discharge pipeline.

Preferably, the transfer trolley comprises:

the device comprises a vehicle body, a stainless steel frame and a control system, wherein the vehicle body is horizontally arranged, universal wheels are fixedly arranged at four corners of the lower end face of the vehicle body, and the stainless steel frame is placed on the vehicle body;

the handle is fixedly arranged on the vehicle body and used for pushing the vehicle body.

Preferably, a trolley is arranged on the trolley body, the trolley is horizontally arranged on the trolley body, and a ceramic sliding roller is arranged at the lower end of the trolley.

Preferably, the cart with still be provided with flexible subassembly between the ceramic smooth roller, flexible subassembly includes:

the central sleeve is vertically arranged on the lower end face of the trolley;

the telescopic shaft is vertically arranged and is arranged in the central sleeve in a sliding manner, the upper end of the telescopic shaft is connected with the bottom wall of the trolley through a first spring, and the lower end of the telescopic shaft is rotatably connected with the ceramic sliding roller;

the brake block is fixedly arranged at the lower end of the central sleeve, and can be abutted against the ceramic sliding roller when the telescopic shaft slides upwards;

the support rods are obliquely arranged, the lower ends of the support rods are hinged with the lower end of the telescopic shaft, the upper ends of the support rods are hinged with support blocks, the support blocks are abutted to the lower end face of the trolley, a plurality of stop blocks are fixedly arranged on the lower end face of the trolley, the stop blocks are arranged in a triangular shape and are arranged in a linear mode, and the support blocks are abutted to one of the stop blocks;

the two telescopic sleeves are symmetrically arranged on two sides of the central sleeve, the telescopic sleeves are vertically arranged, and the upper ends of the telescopic sleeves are fixedly connected with the lower end face of the cart;

the telescopic push rod is vertically arranged and is arranged in the telescopic sleeve in a sliding mode, the upper end of the telescopic push rod is connected with the bottom wall of the trolley through a second spring, the lower end of the telescopic push rod is rotatably provided with a rotating wheel, and the rotating wheel is abutted to the support rod;

the pedal is horizontally arranged, and one end of the pedal is fixedly connected with the telescopic push rod;

one end of the third spring is fixedly connected with the bottom wall of the trolley, and the other end of the third spring is fixedly connected with the support rod;

the connecting rod, the connecting rod level sets up, and the connecting rod both ends respectively with two telescopic push rod fixed connection.

Preferably, still be provided with balanced subassembly on the automobile body, balanced subassembly includes:

the balance box is embedded in the vehicle body, and a balance cavity is formed in the balance box;

the upper central rod is vertically arranged and is arranged at the center of the top wall of the balance box, the upper central rod penetrates through the top wall of the balance box and is rotatably connected with the top wall of the balance box, and the bottom wall of the balance box is also rotatably provided with a lower central rod;

the upper balancing sleeve is sleeved on the upper central rod and is rotatably connected with the top wall of the balancing box, the upper balancing sleeve is rotatably connected with the upper central rod, and the lower central rod is also sleeved with a lower balancing sleeve;

the mounting blocks are fixedly arranged on the upper central rod, the upper balance sleeve, the lower central rod and the lower balance sleeve respectively, mounting convex columns are rotatably arranged on the mounting blocks, and the mounting convex columns are horizontally arranged;

the balance rods are horizontally arranged, one ends of the balance rods are rotationally connected with the mounting convex columns, balance wheels are rotationally arranged at the ends, far away from the mounting convex columns, of the balance rods, and the four balance rods are circumferentially arrayed by taking the axis of the upper center rod as the center at the initial position, namely the two adjacent balance rods are perpendicular to each other;

the three balance plates are horizontally arranged in the balance box at equal intervals from top to bottom, the balance plates are arranged in a ring shape, the four balance wheels roll along the balance plates and the bottom wall of the balance box respectively, the balance rod and the bottom wall of the balance box are arranged into slight concave surfaces, and the lowest position of the concave surfaces is the position of the balance wheel when the initial position is the lowest position;

the outer wall of the upper side of the balance box is fixedly provided with four upper bases, the four upper bases correspond to the four balance wheels in initial positions respectively, the upper bases are in threaded connection with upper foot rods, the upper foot rods are vertically arranged, one ends, far away from the upper bases, of the upper foot rods are rotatably provided with upper foot pads, the outer wall of the lower side of the balance box is also provided with lower bases, the lower bases are also in threaded connection with lower foot rods, the lower foot rods are fixedly connected with lower foot pads, and the upper foot pads and the lower foot pads are abutted to the car body respectively;

the end part of the upper central shaft, which is positioned outside the balance box, is fixedly provided with a first driving sprocket, one of the upper foot rods is fixedly provided with a first driven sprocket, the first driving sprocket and the first driven sprocket are connected and driven by a chain, and the first driven sprocket and the balance wheel on the upper central shaft are in central symmetry with each other;

the end part of the upper balance sleeve, which is positioned outside the balance box, is fixedly provided with a second driving sprocket, one of the upper leg rods is fixedly provided with a second driven sprocket, the second driven sprocket is connected with the second driving sprocket through chain transmission, the second driven sprocket is centrally symmetrical with the central shaft above the balance wheel on the upper balance sleeve, the lower central shaft and the lower balance sleeve are also respectively provided with a third driving sprocket and a fourth driving sprocket, the lower leg rod at the corresponding position is also provided with a third driven sprocket and a fourth driven sprocket, the third driven sprocket is connected with the third driving sprocket through a chain for transmission, and the fourth driving sprocket is connected with the fourth driven sprocket through chain transmission;

the central plate is arranged on the trolley body, a through hole is formed in the upper surface of the trolley body, a vertical connecting rod is arranged at the valley bottom of the upper surface of the balance box, the vertical connecting rod is fixedly connected with the central plate, and the trolley is placed on the trolley body.

Preferably, the microscopic observation system comprises: the device comprises a blocking and stopping assembly, a lifting assembly, an observation assembly, an electric control cabinet, a human-computer interaction assembly and a main body frame, wherein the blocking and stopping assembly is fixedly arranged on the main body frame and used for limiting the position of a tube valve frame, the lifting assembly is arranged on the main body frame and used for controlling the tube valve frame to lift, the observation assembly is arranged on the main body frame and used for observing a cell factory, the human-computer interaction assembly comprises a display, the display is electrically connected with the observation assembly, and the electric control cabinet is fixedly arranged on the main body frame and used for controlling the operation of each assembly.

Preferably, the gear stop assembly includes: the support is fixedly arranged on the main body frame, the micro electric cylinder is arranged on the support, the stop shaft is fixedly connected with the micro electric cylinder, and the linear bearing is arranged on the stop shaft;

the lifting assembly comprises: lifting frame, servo motor and synchronous lift, lifting frame is fixed to be set up on the main body frame, servo motor is fixed to be set up lifting frame is last, four synchronous lift sets up lifting frame four corners, servo motor passes through the synchronous lift of chain drive.

Preferably, the observation assembly comprises two groups of observation modules, each group of observation modules comprising: x axle slip table, Y axle slip table, Z axle electric cylinder and observer, X axle slip table is fixed to be set up on the main body frame, Y axle slip table slides and sets up on the X axle slip table, and Y axle slip table slip direction is X axle slip table length direction, Y axle slip table perpendicular to X axle slip table, Z axle electric cylinder slides and sets up on the Y axle slip table and the slip direction is Y axle slip table length direction, Z axle electric cylinder output shaft perpendicular to X axle slip table and Y axle slip table place plane, the observer is fixed to be set up on the Z axle electric cylinder output shaft, it is two sets of the observation module sets up respectively main body frame upper end and lower extreme, the fixed truckle that is provided with of main body frame lower extreme.

Preferably, a mirror changing assembly is further arranged on the output shaft of the Z-axis electric cylinder, and the mirror changing assembly comprises:

the base plate is fixedly arranged on the output shaft of the Z-axis electric cylinder, the base plate is horizontally arranged, and the observer is fixedly arranged on the lower surface of the base plate;

the lens changing disc is arranged below the base disc and is parallel to the base disc, a first central shaft is fixedly arranged on the lower end face of the base disc and is vertically arranged, the lens changing disc is rotatably arranged on the first central shaft, ratchets are arranged on the outer side of the lens changing disc, a plurality of lenses are arranged on the lens changing disc in a circumferential array mode, and the lenses are located below the observer;

the lens changing motor is fixedly arranged on the lower surface of the base plate and is positioned on the side part of the lens changing plate, an output shaft of the lens changing motor is vertically arranged, a first crank is fixedly arranged on the output shaft of the lens changing motor, one end, far away from the output shaft of the lens changing motor, of the first crank is hinged with a second crank, and one end, far away from the first crank, of the second crank is rotatably provided with a first convex column;

the mirror changing plate comprises a base plate, a first convex column, a second convex column, a shifting block and a second convex column, wherein the base plate is provided with a first angle, the first angle is parallel to the plane of the shifting block, the second angle is parallel to the plane of the shifting block, the first convex column is rotatably connected with one angle of the shifting block, the second convex column is rotatably arranged at the other angle of the shifting block, the upper end of the second convex column is fixedly connected with the base plate, and the last angle of the shifting block is fixedly provided with a third convex column;

the first pawl is rotationally arranged on the third convex column, a torsion spring is arranged between the first pawl and the third convex column, and the first pawl is matched with a ratchet at the outer side of the mirror changing disc to be used for dialing the mirror changing disc;

the second pawl is arranged on the side part of the lens changing disc, a fourth convex column is rotatably arranged on the second pawl, a torsion spring is arranged between the second pawl and the fourth convex column, the upper end of the fourth convex column is fixedly connected with the base disc, the second pawl is stirred by a ratchet when the lens changing disc rotates, and a driving lever is fixedly arranged on the second pawl;

the cleaning box is arranged to be of an L-shaped structure, the upper end of the cleaning box is fixedly connected with the base plate, the lower end of the cleaning box is located right below the lens, a limiting plate is arranged on the cleaning box below the lens, the lower end face of the limiting plate is connected with the cleaning box through a limiting spring, and a plurality of pieces of lens cleaning paper are stacked on the limiting plate;

the horizontal moving rod penetrates through the side wall of the cleaning box and is hinged with the cleaning box, a paper pickup wheel is fixedly arranged at one end, close to the mirror paper, of the horizontal moving rod, the paper pickup wheel is pressed on the mirror paper, and one end, far away from the paper pickup wheel, of the horizontal moving rod is fixedly connected with the shifting lever through a pull rope.

The working principle and the beneficial effects of the invention are as follows: when the cell factory drainage device works, the cell factory is fixed on the pipe valve frame, and meanwhile, the pipe valve pressing plate assembly is fixed on the pipe valve frame and is connected with the cell factory, so that the liquid inlet and outlet of the cell factory are accurately controlled through the peristaltic pump pipe valve of the pipe valve pressing plate assembly, the complex operation that the cell factory is taken down and then drained during manual operation is avoided, and meanwhile, the pipe valve frame is moved by the transfer trolley when needed, so that the problems of high labor intensity and high liquid disturbance during manual operation of the liquid inlet and outlet of the cell factory are solved.

Additional features and advantages of the invention will be set forth in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention. The objectives and other advantages of the invention will be realized and attained by the structure particularly pointed out in the written description and drawings.

The technical solution of the present invention is further described in detail by the accompanying drawings and embodiments.

Drawings

The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the principles of the invention and not to limit the invention. In the drawings:

FIG. 1 is a schematic diagram of a frame structure of a tube valve for preparing a biological product in a cell factory according to an embodiment of the present invention;

FIG. 2 is a schematic diagram of a tube valve pressing plate assembly in a tube valve frame for preparing biological products in a cell factory according to an embodiment of the present invention;

FIG. 3 is a schematic view of a transfer car structure of a pipe valve frame for preparing biological products in a cell factory according to an embodiment of the present invention;

FIG. 4 is a schematic diagram of a telescopic assembly in a tube valve frame for preparing biological products in a cell factory according to an embodiment of the present invention;

FIG. 5 is a first schematic diagram of a balance assembly of a tube valve frame for cell factory preparation of biological products according to an embodiment of the present invention;

FIG. 6 is a schematic diagram of a second configuration of a balancing assembly in a frame of a tube valve for use in a cell factory for preparing a biological product, in accordance with an embodiment of the present invention;

FIG. 7 is a schematic structural diagram of a microscopic observation system according to an embodiment of the present invention;

FIG. 8 is a schematic diagram of a stop assembly in a microscopic observation system according to an embodiment of the present invention;

FIG. 9 is a schematic diagram of a lifting assembly of a microscopic observation system according to an embodiment of the present invention;

FIG. 10 is a schematic view of a viewing assembly of a microscopy viewing system according to an embodiment of the present invention;

FIG. 11 is a schematic view of the structure of the main frame and the caster of the microscopic observation system according to the embodiment of the present invention;

FIG. 12 is a first schematic view of a microscope objective system according to an embodiment of the present invention;

fig. 13 is a schematic structural diagram of a microscope changer assembly in a microscopic observation system according to an embodiment of the present invention.

The reference numbers in the drawings are as follows: 1. a tube valve platen assembly; 11. a platen box; 12. a peristaltic pump tube valve; 13. a stainless steel frame; 2. a mirror changing assembly; 201. a base plate; 202. changing a mirror plate; 203. a first central shaft; 204. a lens; 205. a mirror-changing motor; 206. a first crank; 207. a first convex column; 208. a shifting block; 209. a second convex column; 210. a third convex column; 211. a first pawl; 212. a second pawl; 213. a fourth convex column; 214. a deflector rod; 215. a cleaning box; 216. a limiting plate; 217. a limiting spring; 218. a translation rod; 219. a paper rolling wheel; 220. pulling a rope; 221. a second crank; 3. a transfer trolley; 31. a vehicle body; 32. a universal wheel; 33. a handle; 34. pushing a cart; 35. a ceramic slip roll; 4. a gear stop assembly; 41. a micro-motion electric cylinder; 42. a support; 43. stopping the shaft; 44. a linear bearing; 5. a lifting assembly; 51. a lifting frame; 52. a servo motor; 53. a synchronous lifter; 6. an observation assembly; 61. an observation module; 611. an X-axis sliding table; 612. a Y-axis sliding table; 613. a Z-axis electric cylinder; 614. an observer; 7. a main body frame; 71. an electric control cabinet; 72. a human-computer interaction component; 721. a display; 73. a caster wheel; 8. a telescoping assembly; 801. a central sleeve; 802. a telescopic shaft; 803. a first spring; 804. a brake block; 805. a stay bar; 806. a support block; 807. a stopper; 808. a telescopic sleeve; 809. a telescopic push rod; 810. a second spring; 811. a rotating wheel; 812. a pedal; 813. a third spring; 814. a connecting rod; 9. a balancing component; 901. an upper center rod; 902. a lower center pole; 903. an upper balance sleeve; 904. a lower balance sleeve; 905. mounting blocks; 906. installing a convex column; 907. a balancing pole; 908. a balance wheel; 909. a balance plate; 910. an upper base; 911. an upper foot bar; 912. mounting a foot pad; 913. a lower base; 914. a lower leg rod; 915. a foot pad is arranged; 916. a first drive sprocket; 917. a first driven sprocket; 918. a second drive sprocket; 919. a second driven sprocket; 920. a third drive sprocket; 921. a third driven sprocket; 922. a fourth drive sprocket; 923. a fourth driven sprocket; 924. a center plate; 925. a vertical connecting rod; 926. and a balancing box.

Detailed Description

The preferred embodiments of the present invention will be described in conjunction with the accompanying drawings, and it will be understood that they are described herein for the purpose of illustration and explanation and not limitation.

The embodiment of the invention provides a tube valve frame and a microscopic observation system for preparing biological products in a cell factory, as shown in figure 1, comprising: the pipe valve pressing plate assembly 1 is arranged on the stainless steel frame 13 and is communicated with a cell factory pipeline, the pipe valve pressing plate assembly 1 is used for controlling liquid feeding and discharging of a cell factory, the stainless steel frame 13 is arranged on the transfer trolley 3, and a microscopic observation system is arranged in the stainless steel frame 13.

The working principle and the beneficial technical effects of the technical scheme are as follows: when the cell factory drainage device works, a cell factory is fixed on a pipe valve frame, and meanwhile, a pipe valve pressing plate assembly 1 is fixed on the pipe valve frame and is connected with the cell factory, so that the liquid inlet and outlet of the cell factory are accurately controlled through a peristaltic pump pipe valve 12 of the pipe valve pressing plate assembly 1, the complex operation that the cell factory is taken down and then drained during manual operation is avoided, and meanwhile, the pipe valve frame is moved through a transfer trolley 3 when needed, so that the problems of high labor intensity and high liquid disturbance during manual operation of the liquid inlet and outlet of the cell factory are solved.

In one embodiment, as shown in fig. 2, the tube valve pressing plate assembly 1 includes a pressing plate box 11, the pressing plate box 11 is clamped with the stainless steel frame 13, six peristaltic pump tube valves 12 are fixedly disposed on the pressing plate box 11, four of the peristaltic pump tube valves 12 are respectively used for controlling the opening and closing of the cell factory pipeline, and the other two peristaltic pump tube valves 12 are respectively used for controlling the opening and closing of the main liquid inlet pipeline and the main liquid outlet pipeline.

The working principle and the beneficial technical effects of the technical scheme are as follows: when the pipe valve frame is used, the pipe valve pressing plate assembly 1 is clamped with the side wall of the cell factory frame through the buckle structure, silica gel is paved on the inner side of the pressing plate, the pressing plate is guaranteed to be in elastic contact with the cell factory, the cell factory is guaranteed to be compressed, meanwhile, stress damage to the cell factory can not be caused in the operation process, manual disassembly and maintenance are facilitated, six pneumatic pipe valves are arranged on the pressing plate, four pneumatic pipe valves are used for controlling the opening and closing of the pipelines of each cell factory, and the other two pneumatic pipe valves are used for controlling the opening and closing of the main liquid inlet pipeline and the main liquid discharge pipeline respectively. Six pneumatic valves are controlled by the signal of the robot control system to be opened and closed in a time-sharing manner, so that accurate and quantitative liquid feeding and discharging of each cell factory can be guaranteed, independent liquid feeding and discharging main pipelines can be adopted, liquid feeding and discharging mixing neutralization is avoided, the reagent dosage is reduced, and the cost is saved.

In one embodiment, as shown in fig. 3, the trolley 3 comprises:

the vehicle body 31 is horizontally arranged, universal wheels 32 are fixedly arranged at four corners of the lower end face of the vehicle body 31, and the stainless steel frame 13 is placed on the vehicle body 31;

and the handle 33 is fixedly arranged on the vehicle body 31 and used for pushing the vehicle body 31.

The working principle and the beneficial technical effects of the technical scheme are as follows: by arranging the transfer trolley 3 and arranging the pipe valve frame on the transfer trolley 3, the trolley body 31 can be pushed by the handle 33 when needed, so that the pipe valve frame is transported, and the problem of large disturbance in manual transportation is avoided.

In one embodiment, a cart 34 is disposed on the vehicle body 31, the cart 34 is horizontally disposed on the vehicle body 31, and a ceramic slide roller 35 is disposed at the lower end of the cart 34.

The working principle and the beneficial technical effects of the technical scheme are as follows: by arranging the cart 34, the cart body 31 can be separated from the cart 34, the cart 34 and the pipe valve frame can be pushed into an observation system under the condition of microscopic observation, the cart body 31 does not need to enter the microscopic observation system, and the cell factory is prevented from being disturbed by shaking caused in the moving process.

In one embodiment, a telescopic assembly 8 is further disposed between the cart 34 and the ceramic roller 35, as shown in fig. 4, the telescopic assembly 8 includes:

the central sleeve 801 is vertically arranged on the lower end face of the trolley 34;

the telescopic shaft 802 is vertically arranged and slidably arranged in the central sleeve 801, the upper end of the telescopic shaft 802 is connected with the bottom wall of the cart 34 through a first spring 803, and the lower end of the telescopic shaft 802 is rotatably connected with the ceramic sliding roller 35;

the brake block 804 is fixedly arranged at the lower end of the central sleeve 801, and the brake block 804 can be abutted against the ceramic sliding roller 35 when the telescopic shaft 802 slides upwards;

the supporting rod 805 is obliquely arranged, the lower end of the supporting rod 805 is hinged to the lower end of the telescopic shaft 802, the upper end of the supporting rod 805 is hinged to a supporting block 806, the supporting block 806 is abutted to the lower end face of the trolley 34, a plurality of stoppers 807 are fixedly arranged on the lower end face of the trolley 34, the stoppers 807 are arranged in a triangular and linear arrangement, and the supporting block 806 is abutted to one of the stoppers 807;

the two telescopic sleeves 808 are symmetrically arranged on two sides of the central sleeve 801, the telescopic sleeves 808 are vertically arranged, and the upper ends of the telescopic sleeves 808 are fixedly connected with the lower end face of the cart 34;

the telescopic push rod 809 is vertically arranged and slidably arranged in the telescopic sleeve 808, the upper end of the telescopic push rod 809 is connected with the bottom wall of the cart 34 through a second spring 810, the lower end of the telescopic push rod 809 is rotatably provided with a rotating wheel 811, and the rotating wheel 811 is abutted to the support rod 805;

the pedal 812 is horizontally arranged, and one end of the pedal 812 is fixedly connected with the telescopic push rod 809;

one end of the third spring 813 is fixedly connected with the bottom wall of the cart 34, and the other end of the third spring 813 is fixedly connected with the support bar 805;

and the connecting rod 814 is horizontally arranged, and two ends of the connecting rod 814 are respectively and fixedly connected with the two telescopic push rods 809.

The working principle and the beneficial technical effects of the technical scheme are as follows: when the cart 34 is placed on the cart body 31, the telescopic shaft is retracted into the central sleeve 801, the brake block 804 abuts against the ceramic slide roller 35, so that the cell factory is prevented from shaking due to the rotation of the ceramic slide roller 35, when the cart 34 needs to be taken down, the pedal 812 is stepped by a foot, the pedal 812 drives the telescopic push rod 809 to move downwards, the rotating wheel 811 abuts against the support rod 805 when the telescopic push rod 809 moves downwards to drive the support rod 805 to move downwards, the telescopic shaft 802 is driven to move downwards to extend out when the support rod 805 moves downwards, the support block 806 is separated from the current stop block 807, when the telescopic shaft 802 extends out until the ceramic slide roller 35 just contacts with the ground, the support block 806 abuts against the stop block 807 at the existing position under the pulling action of the third spring 813, so that the support of the telescopic shaft 802 is completed, and the cart 34 above cannot displace in a large range in the vertical direction, thereby avoiding greater disturbances to the cell factory.

In one embodiment, the vehicle body 31 is further provided with a balancing assembly 9, as shown in fig. 5 and 6, the balancing assembly 9 includes:

the balance box 926, the balance box 926 is embedded in the vehicle body 31, and a balance cavity is formed in the balance box 926;

the upper central rod 901 is vertically arranged and arranged at the center of the top wall of the balance box 926, the upper central rod 901 penetrates through the top wall of the balance box 926 and is rotatably connected with the top wall of the balance box 926, and the bottom wall of the balance box 926 is also rotatably provided with a lower central rod 902;

an upper balance sleeve 903, wherein the upper balance sleeve 903 is sleeved on the upper central rod 901, the upper balance sleeve 903 is rotatably connected with the top wall of the balance box 926, the upper balance sleeve 903 is rotatably connected with the upper central rod 901, and the lower central rod 902 is also sleeved with a lower balance sleeve 904;

the mounting block 905 is fixedly arranged on each of the upper center rod 901, the upper balance sleeve 903, the lower center rod 902 and the lower balance sleeve 904, a mounting convex column 906 is rotatably arranged on each mounting block 905, and the mounting convex column 906 is horizontally arranged;

the balance rods 907 are horizontally arranged, one end of each balance rod 907 is rotatably connected with the corresponding mounting convex column 906, one end, far away from the corresponding mounting convex column 906, of each balance rod 907 is rotatably provided with a balance wheel 908, and in an initial position, the four balance rods 907 are arranged in a circumferential array with the axis of the upper center rod 901 as the center, namely the two adjacent balance rods 907 are perpendicular to each other;

the balance plates 909, three balance plates 909 are horizontally arranged in the balance box 926 from top to bottom at equal intervals, the balance plates 909 are arranged in a ring shape, four balance wheels 908 respectively roll along the balance plates 909 and the bottom wall of the balance box 926, the balance bar 907 and the bottom wall of the balance box 926 are arranged to be slightly concave, and the lowest part of the concave surface is the position of the balance wheels 908 at the initial position;

an upper foot pad 912, wherein four upper bases 910 are fixedly arranged on the outer wall of the upper side of the balancing tank 926, the four upper bases 910 respectively correspond to the initial positions of the four balancing wheels 908, an upper foot rod 911 is connected to the upper base 910 through a thread, the upper foot rod 911 is vertically arranged, an upper foot pad 912 is rotatably arranged at one end, away from the upper base 910, of the upper foot rod 911, a lower base 913 is also arranged on the outer wall of the lower side of the balancing tank 926, a lower foot rod 914 is also connected to the lower base 913 through a thread, a lower foot pad 915 is fixedly connected to the lower foot rod 914, and the upper foot pad 912 and the lower foot pad 915 are respectively abutted against the vehicle body 31;

a first driving sprocket 916, the end of the upper central shaft located outside the balancing box 926 is fixedly provided with the first driving sprocket 916, one of the upper leg bars 911 is fixedly provided with a first driven sprocket 917, the first driving sprocket 916 and the first driven sprocket 917 are in transmission connection through a chain, and the first driven sprocket 917 and the balancing wheel 908 on the upper central shaft are in central symmetry with respect to the upper central shaft;

a second driving sprocket 918 is fixedly arranged at the end part of the upper balance sleeve 903 positioned outside the balance box 926, a second driven sprocket 919 is fixedly arranged on one of the upper foot bars 911, the second driven sprocket 919 is in chain transmission connection with the second driving sprocket 918, the second driven sprocket 919 is in central symmetry with the balance wheel 908 on the upper balance sleeve 903, the upper central shaft and the lower balance sleeve 904 are also respectively provided with a third driving sprocket 920 and a fourth driving sprocket 922, the lower foot bar 914 at the corresponding positions is also provided with a third driven sprocket 921 and a fourth driven sprocket 923, the third driven sprocket 921 and the third driving sprocket 920 are in chain connection transmission, and the fourth driving sprocket 922 and the fourth driven sprocket 923 are in chain transmission connection;

the central plate 924 is arranged on the car body 31, a through hole is formed in the upper surface of the car body 31, a vertical connecting rod 925 is arranged at the valley bottom of the upper surface of the balance box 926, the vertical connecting rod 925 is vertically arranged and fixedly connected with the central plate 924, and the cart 34 is placed on the car body 31.

The working principle and the beneficial technical effects of the technical scheme are as follows: when the vehicle body 31 moves to a slightly inclined ground, the balance wheel 908 moves away from the initial position toward the lowest position under the action of gravity, the balance wheel 907 drives the upper central rod 901, the upper balance sleeve 903, the lower central rod 902 and the lower balance sleeve 904 to rotate respectively in the movement process, then drives the corresponding chain wheels to rotate respectively, and drives the corresponding upper foot bar 911 or lower foot bar 914 to rotate through chain transmission of the chain wheels, and as the upper foot bar 911 is in threaded connection with the upper base 910, the lower foot bar 914 is in threaded connection with the lower base 913, the extending length of the upper foot bar 911 or lower foot bar 914 is adjusted, so that the balance box 926 is always in a balance position, the balance plate 909 is always horizontal, the cart 34 placed on the central plate 924 is always horizontal, and the influence on a cell factory in the inclined state is avoided.

An embodiment of the present invention further provides a microscopic observation system, as shown in fig. 7, including: the device comprises a stopping component 4, a lifting component 5, an observing component 6, an electric control cabinet 71, a human-computer interaction component 72 and a main body frame 7, wherein the stopping component 4 is fixedly arranged on the main body frame 7 and used for limiting the position of a tube valve frame, the lifting component 5 is arranged on the main body frame 7 and used for controlling the lifting of the tube valve frame, the observing component 6 is arranged on the main body frame 7 and used for observing a cell factory, the human-computer interaction component 72 comprises a display 721, the display 721 is electrically connected with the observing component 6, and the electric control cabinet 71 is fixedly arranged on the main body frame 7 and used for controlling the operation of all components.

The working principle and the beneficial technical effects of the technical scheme are as follows: when the cell factory frame is used, an operator pushes the frame, the trolley 34 of the transfer trolley 3 is separated from the trolley body 31 and enters the interior of the microscopic observation system, when the frame completely enters the interior of the main body frame 7 of the microscopic observation system, the in-place sensor sends a signal to control the stopping assembly 4 to carry out position limiting and locking on the cell factory frame, and after the stopping assembly 4 carries out position limiting and locking on the cell factory frame, the operator can operate the lifting assembly 5 on the human-computer interaction assembly 72 to lift the cell factory frame to an observation position. Upon reaching the observation location, the location sensor signals that the operator controls the observation assembly 6 to observe the cell factory. After observation is finished, the operator resets the lifting assembly 5, the blocking assembly 4 is unlocked, and after the blocking assembly 4 is unlocked, the human-computer interaction assembly 72 prompts the operator to pull out the cell factory frame, the rotary trolley 3 is replaced, then the operator pushes the cell factory frame back to the culture room or the next operation by using the transfer trolley 3, and microscopic observation operation is finished.

In one embodiment, as shown in fig. 8 and 9, the stop assembly 4 comprises: the device comprises a micro electric cylinder 41, a bracket 42, a stop shaft 43 and a linear bearing 44, wherein the bracket 42 is fixedly arranged on the main body frame 7, the micro electric cylinder 41 is arranged on the bracket 42, the stop shaft 43 is fixedly connected with the micro electric cylinder 41, and the linear bearing 44 is arranged on the stop shaft 43;

the lifting assembly 5 comprises: the lifting frame 51 is fixedly arranged on the main body frame 7, the servo motors 52 are fixedly arranged on the lifting frame 51, the four synchronous lifters 53 are arranged at four corners of the lifting frame 51, and the servo motors 52 drive the synchronous lifters 53 through chain transmission.

The working principle and the beneficial technical effects of the technical scheme are as follows: when the pipe valve frame enters the microscopic observation system frame main body, the micro-electro-cylinder 41 is started to drive the pushing stop shaft 43 to extend out, so that the pipe valve frame is abutted and limited, and the observation accuracy is prevented from being influenced by movement during observation; when the lifting assembly 5 is in operation, the servo motor 52 is started to drive the synchronous lifters 53 through chain transmission, and the synchronous lifters 53 push the cart 34 to move up and down from four corners at the same time, so as to drive the cell factory frame to move up and down synchronously. Thereby precisely lifting the cell factory frame to the observation position for the staff to observe the cell growth state in the cell factory by the camera in the observation assembly 6.

In one embodiment, as shown in fig. 10 and 11, the viewing assembly 6 comprises two sets of viewing modules 61, each set of viewing modules 61 comprising: the X-axis sliding table 611 is fixedly arranged on the main body frame 7, the Y-axis sliding table 612 is slidably arranged on the X-axis sliding table 611, the sliding direction of the Y-axis sliding table 612 is the length direction of the X-axis sliding table 611, the Y-axis sliding table 612 is perpendicular to the X-axis sliding table 611, the Z-axis electric cylinder 613 is slidably arranged on the Y-axis sliding table 612 and is the length direction of the Y-axis sliding table 612, the output shaft of the Z-axis electric cylinder 613 is perpendicular to the plane where the X-axis sliding table 611 and the Y-axis sliding table 612 are located, the observer 614 is fixedly arranged on the output shaft of the Z-axis electric cylinder 613, the two groups of observation modules 61 are respectively arranged at the upper end and the lower end of the main body frame 7, and the lower end of the main body frame 7 is fixedly provided with a caster 73.

The working principle and the beneficial technical effects of the technical scheme are as follows: observe subassembly 6 during operation, through setting up X axle slip table 611, Y axle slip table 612 and the electric jar 613 of Z axle that mutual slip set up, can drive observer 614 and realize the arbitrary direction removal in the three-dimensional space to can realize surveing the cell factory of optional position, simultaneously, through setting up truckle 73, can conveniently remove cell factory, hoisting device's practicality.

In an embodiment, a switching mirror assembly 2 is further disposed on the output shaft of the Z-axis electric cylinder 613, as shown in fig. 12 and 13, the switching mirror assembly 2 includes:

the base plate 201, the base plate 201 is fixedly arranged on the output shaft of the Z-axis electric cylinder 613, the base plate 201 is horizontally arranged, and the observer 614 is fixedly arranged on the lower surface of the base plate 201;

the lens changing disc 202 is arranged below the base disc 201 and is parallel to the base disc 201, a first central shaft 203 is fixedly arranged on the lower end surface of the base disc 201, the first central shaft 203 is vertically arranged, the lens changing disc 202 is rotatably arranged on the first central shaft 203, ratchets are arranged on the outer side of the lens changing disc 202, a plurality of lenses 204 are arranged on the lens changing disc 202 in a circumferential array manner, and the lenses 204 are positioned below the observer 614;

the mirror changing motor 205 is fixedly arranged on the lower surface of the base plate 201 and located on the side portion of the mirror changing plate 202, an output shaft of the mirror changing motor 205 is vertically arranged, a first crank 206 is fixedly arranged on the output shaft of the mirror changing motor 205, one end, far away from the output shaft of the mirror changing motor 205, of the first crank 206 is hinged to a second crank 221, and one end, far away from the first crank 206, of the second crank 221 is rotatably provided with a first convex column 207;

the shifting block 208 is triangular, the plane where the shifting block 208 is located is parallel to the mirror changing disc 202, the first convex column 207 is rotationally connected with one corner of the shifting block 208, the other corner of the shifting block 208 is rotationally provided with a second convex column 209, the upper end of the second convex column 209 is fixedly connected with the base disc 201, and the last corner of the shifting block 208 is fixedly provided with a third convex column 210;

the first pawl 211 is rotatably arranged on the third convex column 210, a torsion spring is arranged between the first pawl 211 and the third convex column 210, and the first pawl 211 is matched with a ratchet on the outer side of the mirror changing plate 202 to be used for rotating the mirror changing plate 202;

the second pawl 212 is arranged on the side of the mirror changing plate 202, a fourth convex column 213 is rotatably arranged on the second pawl 212, a torsion spring is arranged between the second pawl 212 and the fourth convex column 213, the upper end of the fourth convex column 213 is fixedly connected with the base plate 201, the second pawl 212 is shifted by a ratchet when the mirror changing plate 202 rotates, and a shift lever 214 is fixedly arranged on the second pawl 212;

the cleaning box 215 is of an L-shaped structure, the upper end of the cleaning box 215 is fixedly connected with the base plate 201, the lower end of the cleaning box 215 is located under the lens 204, a limiting plate 216 is arranged on the cleaning box 215 under the lens 204, the lower end face of the limiting plate 216 is connected with the cleaning box 215 through a limiting spring 217, and a plurality of pieces of lens wiping paper are stacked on the limiting plate 216;

the horizontal moving rod 218 penetrates through the side wall of the cleaning box 215 and is hinged with the cleaning box 215, a paper pickup wheel 219 is fixedly arranged at one end, close to the mirror paper, of the horizontal moving rod 218, the paper pickup wheel 219 is pressed on the mirror paper, and one end, far away from the paper pickup wheel 219, of the horizontal moving rod 218 is fixedly connected with the shifting rod 214 through a pull rope 220.

The working principle and the beneficial technical effects of the technical scheme are as follows: when the microscopic observation system is used, one of the lenses 204 is positioned under the observer 614, the magnification can be better adjusted by the arrangement of the observer 614 and the lenses 204, and better observation can be carried out, when the observation magnification needs to be further adjusted, the mirror changing motor 205 is started and drives the first crank 206 to rotate, the first crank 206 drives the second crank 221 to swing when rotating, and further drives the shifting block 208 to swing around the second convex column 209, the shifting block 208 drives the first pawl 211 to shift the mirror changing disc 202 to rotate when swinging, so that the lens 204 with another different magnification is positioned under the observer 614, meanwhile, when the mirror changing disc 202 rotates, the lens 204 is wiped by the wiping paper, so that the lens 204 is prevented from being blocked by impurities, when the mirror changing disc 202 rotates, the second pawl 212 is shifted, the second pawl 212 pulls the flat moving rod 218 through the shift lever 214 and the pull rope 220, when the flat moving rod 218 horizontally swings, the paper wheel 219 pulls the uppermost layer of the wiping paper aside, thereby avoiding the contamination of the lens 204 behind by the repeated use of the same piece of lens-wiping paper.

It will be apparent to those skilled in the art that various changes and modifications may be made in the present invention without departing from the spirit and scope of the invention. Thus, if such modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include such modifications and variations.

Claims

1. A tube valve frame and microscopic observation system for preparing biological products in a cell factory, comprising: the tube valve pressing plate assembly (1) is arranged on the stainless steel frame (13) and is communicated with a cell factory pipeline, the tube valve pressing plate assembly (1) is used for controlling liquid inlet and outlet of a cell factory, the stainless steel frame (13) is arranged on the transfer trolley (3), and a microscopic observation system is arranged in the stainless steel frame (13);

the pipe valve pressing plate assembly (1) comprises a pressing plate box (11), the pressing plate box (11) is clamped with the stainless steel frame (13), six peristaltic pump pipe valves (12) are fixedly arranged on the pressing plate box (11), wherein four peristaltic pump pipe valves (12) are respectively used for controlling the opening and closing of a cell factory pipeline, and the other two peristaltic pump pipe valves (12) are respectively used for controlling the opening and closing of a main liquid inlet pipeline and a main liquid outlet pipeline;

the transfer trolley (3) comprises:

the trolley comprises a trolley body (31), wherein the trolley body (31) is horizontally arranged, universal wheels (32) are fixedly arranged at four corners of the lower end face of the trolley body (31), and the stainless steel frame (13) is placed on the trolley body (31);

the handle (33), the said handle (33) is fixedly arranged on the said vehicle body (31) and is used for pushing the said vehicle body (31);

a trolley (34) is arranged on the trolley body (31), the trolley (34) is horizontally arranged on the trolley body (31), and a ceramic sliding roller (35) is arranged at the lower end of the trolley (34);

shallow (34) with still be provided with flexible subassembly (8) between ceramic smooth roller (35), flexible subassembly (8) include:

a center sleeve (801), wherein the center sleeve (801) is vertically arranged on the lower end face of the cart (34);

the telescopic shaft (802) is vertically arranged and slidably arranged in the central sleeve (801), the upper end of the telescopic shaft (802) is connected with the bottom wall of the trolley (34) through a first spring (803), and the lower end of the telescopic shaft (802) is rotatably connected with the ceramic sliding roller (35);

the brake block (804) is fixedly arranged at the lower end of the central sleeve (801), and the brake block (804) can be abutted against the ceramic sliding roller (35) when the telescopic shaft (802) slides upwards;

the support rod (805) is obliquely arranged, the lower end of the support rod (805) is hinged to the lower end of the telescopic shaft (802), the upper end of the support rod (805) is hinged to a support block (806), the support block (806) is abutted to the lower end face of the trolley (34), a plurality of blocks (807) are fixedly arranged on the lower end face of the trolley (34), the blocks (807) are arranged in a triangular and linear arrangement mode, and the support block (806) is abutted to one of the blocks (807);

the two telescopic sleeves (808) are symmetrically arranged on two sides of the central sleeve (801), the telescopic sleeves (808) are vertically arranged, and the upper ends of the telescopic sleeves (808) are fixedly connected with the lower end face of the cart (34);

the telescopic push rod (809) is vertically arranged and is arranged in the telescopic sleeve (808) in a sliding manner, the upper end of the telescopic push rod (809) is connected with the bottom wall of the trolley (34) through a second spring (810), the lower end of the telescopic push rod (809) is rotatably provided with a rotating wheel (811), and the rotating wheel (811) is abutted against the stay bar (805);

the pedal (812) is horizontally arranged, and one end of the pedal (812) is fixedly connected with the telescopic push rod (809);

one end of the third spring (813) is fixedly connected with the bottom wall of the trolley (34), and the other end of the third spring (813) is fixedly connected with the support rod (805);

the connecting rod (814) is horizontally arranged, and two ends of the connecting rod (814) are respectively fixedly connected with the two telescopic push rods (809).