CN114015554A - High-flux monoclonal automatic selection module - Google Patents

High-flux monoclonal automatic selection module Download PDF

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CN114015554A
CN114015554A CN202111412929.1A CN202111412929A CN114015554A CN 114015554 A CN114015554 A CN 114015554A CN 202111412929 A CN202111412929 A CN 202111412929A CN 114015554 A CN114015554 A CN 114015554A
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picking head
picking
monoclonal
head
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陈皓
杨开琳
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Innovel Intelligent Technology Suzhou Co Ltd
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Abstract

The invention relates to the technical field of biological pharmacy, in particular to a high-throughput monoclonal automatic selection module which comprises a multichannel picking head core unit, a mobile X-Y linear motor platform and a multichannel picking head cleaning unit, and aims to provide the high-throughput monoclonal automatic selection module, realize the selection of tens of thousands of monoclonal samples per day, meet the selection requirement of a large number of monoclonal samples in the high-throughput research and development process, effectively reduce the cost in the single sample process, in particular, the cost of disposable consumables can be eliminated, the total fixed asset purchasing and daily use cost is comprehensively improved, an automatic system needs to have good identification capability on different types of carriers such as bacteria/cells/yeast and the like, so as to meet the effectiveness in different application scenes of biological medicines, clinical medicine, foods, synthetic biology and the like.

Description

High-flux monoclonal automatic selection module
Technical Field
The invention relates to the technical field of biological pharmacy, in particular to a high-throughput monoclonal automatic selection module.
Background
Biological medicines, synthetic biology and the like are in a high-speed development stage, a gene synthesis process is used as an important research and development means and is more and more widely applied, after gene synthesis, genes are transferred into amplification carriers such as target bacteria/cells, quick identification and selection of monoclone are a crucial link so as to effectively amplify the genes and matched products, the traditional manual selection mode is high in labor intensity and easy to fatigue, the manual selection amount and the industrial research and development demand amount are 1-2 orders of magnitude different, a huge gap exists relative to the sample demand of the industrial research and development, a disposable gun head is matched with an existing liquid transfer workstation, the related functions of monoclone selection can be realized, the cost of disposable gun head consumables is reduced, the cost of the whole flow of monoclone selection and gene synthesis is greatly increased, and the disposable consumables are stored and transported, an industrial transfer robot is required to be arranged in a large-scale high-throughput system, Supporting facilities such as consumptive material carrier, consumptive material cleanliness factor holding unit further improve user's overall use cost.
The invention aims to provide a high-throughput monoclonal automatic selection module, which can realize selection of tens of thousands of monoclonal samples per day, meet the requirement of selection of a large number of monoclonal samples in a high-throughput research and development process, effectively reduce the cost in a single sample process, particularly eliminate the cost of disposable consumables, comprehensively improve the cost of purchasing and daily use of total fixed assets, and ensure that an automatic system has good identification capability on carriers of different types of bacteria/cells/yeasts and the like so as to meet the effectiveness in different application scenes of biological medicines, clinical medicine, food, synthetic biology and the like.
Disclosure of Invention
The present invention is directed to a high throughput automatic monoclonal sorting module, which solves the above problems.
In order to achieve the purpose, the invention provides the following technical scheme:
the utility model provides a module is selected in high flux monoclonal automation, includes that the multichannel is chosen first core unit, removes X-Y linear electric motor platform and multichannel and choose first cleaning unit, the multichannel is chosen first core unit and is included unit rectification housing, Z axle motor, distribution valve piece, collection dress gas circuit switch valves, pneumatic flexible subassembly, is chosen first connecting piece, is chosen first, distance sensor, camera and support, the multichannel is chosen first cleaning unit and is included to choose first alcohol washing tank 1#, is chosen first alcohol washing tank 2#, is chosen first alcohol washing tank 3#, is chosen first drying tank, air intake 1#, air intake 2#, heats unit array and air exit.
As a preferable scheme of the invention, the tail end of the picking head is provided with an adsorption groove, the optimal picking effect and transfer safety can be achieved, the picking head is prevented from falling off in the carrying process, the picking head is made of high-performance stainless steel meeting GMP requirements and is resistant to common sterilizing agents, the guide part of the picking head is made of high-wear-resistant materials, the FDA certification is met, the dust and particulate pollution in the motion process is prevented, the picking head (17) and the driving mechanism are connected and arranged in a floating compensation mode, the processing and assembling difficulty can be reduced on the premise of ensuring the relative precision of the picking head in multiple channels, the driving unit and the control element of the picking head are designed in a modularization mode, the random flux integration from a single channel to a 96 channel can be flexibly realized, the picking head in the multiple channels is subjected to unified tail end hard limiting, the plane formed by one-step processing ensures the accuracy of the descending distance of the picking head, and the mounting hole of the multiple channels of the picking head, the one-step forming guarantees the accuracy of the relative position of the picking head in the multiple channels, and the multiple-channel driving part fixing mechanism adopts the modular design, so that the integration of the single channel to the 96 channels with any flux can be flexibly realized.
As a preferable scheme of the invention, the Z-axis motor can drive the picking head to realize the movement in the Z-axis direction, and a motor brake device is arranged in the Z-axis motor, so that the self-locking can be realized under the condition of power failure, and the safety in the operation process is ensured.
As the preferable scheme of the invention, the camera is a high-resolution visual camera and is provided with a magnifying focusing lens, so that the local magnifying function can be realized for accurately positioning the monoclonal sample, and the camera is provided with a specially matched visual recognition algorithm, so that the colony with the minimum size of 0.05mm can be recognized and positioned.
As a preferable scheme of the present invention, the distance sensor may use different types of sensors such as laser and ultrasound, preferably a high-precision laser displacement sensor, and can realize distance recognition of a minimum 2um, accurately control the descending height of the picking head, and realize colony selection.
As a preferred scheme of the invention, the basic process of monoclonal selection is as follows: firstly, moving an X-Y linear motor platform to transfer a multi-channel picking head core unit to a target hole plate position to finish primary aiming, moving the X-Y linear motor platform to drive the multi-channel picking head core unit to move, completing flatness scanning of a selecting plate and calibrating height distance by a distance sensor, simultaneously shooting a real-time picture by a camera to complete real-time position calculation of target bacteria or cells, controlling a computer to complete selecting sequence scheduling, then selecting the position of the target bacteria or cells, moving the X-Y linear motor platform to an accurate aiming position, quickly confirming the position by a camera, micro-adjusting a Z-axis motor to match the local height of the selecting position, assembling a single-channel air valve in an air channel switch valve group to complete up-and-down movement of a single-channel picking head, realizing selecting operation, selecting the next single-clone selecting target, and repeating the operation of the previous step, until the 96-well plate completed the elution of the whole plate monoclonal picked product.
As a preferable scheme of the invention, after the single clone of each batch is selected, the multichannel picking head core unit needs to be deeply cleaned so as to avoid cross contamination among batches, and after sterilization, the multichannel picking head core unit needs to be effectively cleaned with a sterilization liquid, and after sterilization, the normal temperature of the picking head is ensured, and the safety of target bacteria/cells is ensured.
As a preferable scheme of the invention, the deep cleaning comprises the following specific steps: will choose the head and send into in proper order and choose first alcohol washing tank 1#, choose first alcohol washing tank 2# and choose first alcohol washing tank 3# in carry out the alcohol cleaning that circulates, heat the unit array and carry out the heat source to choosing the head and shine the stoving, the air flows in and discharges through the air exit through air intake 1# and air intake 2#, the air of flow can cool off and choose the head.
As a preferred embodiment of the present invention, the multi-channel picking core unit may configure 1, 2, 4, 8, 16, 24, 48, 96, etc. picking heads with different numbers based on the process requirements, and the multi-channel picking cleaning unit may configure 1, 2, or 3 picking head alcohol cleaning tanks based on the process requirements.
As a preferable scheme of the invention, in the process of monoclonal selection, when the picking head moves horizontally, the distance sensor can measure the thickness of the agar coating on the target orifice plate in real time, the Z-axis motor can drive the picking head to move up and down according to a measuring mechanism of the distance sensor, and the distance from the picking head to the agar coating on the target orifice plate can be kept constant.
Compared with the prior art, the invention has the beneficial effects that:
1. in the invention, a multichannel picker core unit is transferred to a target orifice plate position by starting a movable X-Y linear motor platform to complete primary aiming, the movable X-Y linear motor platform drives the multichannel picker core unit to move, a distance sensor completes flatness scanning of a selection plate and calibrates height distance, a camera simultaneously carries out real-time picture shooting to complete real-time position calculation of target bacteria or cells, a computer is controlled to complete selection sequence scheduling, then the position of the target bacteria or cells is selected, the X-Y linear motor platform is moved to an accurate aiming position, a camera quickly confirms the position, a Z-axis motor carries out micro-adjustment to match local height of the selection position, a single-channel air valve in an air channel switch valve group is assembled to move, the up-and-down movement of a single-channel picker is completed, the selection operation is realized, and the next monoclonal selection target is selected, the operation of the previous step is repeated until the elution of the whole plate monoclonal selection product is completed by a 96-well plate, the flexibility and the process adaptability which are comparable to those of manual selection are realized, compared with a manual selection mode, the efficiency of 1-2 orders of magnitude can be improved by a single device, and the effective identification and the positioning of different cell/bacterium/yeast/fungus samples are realized by applying machine vision and artificial intelligence identification, so that the device can be effectively adapted to different surfaces/surface flatness and different consumable materials such as selection round dishes, agar plates and the like.
2. According to the invention, the picking head is sequentially sent into the picking head alcohol cleaning tank 1#, the picking head alcohol cleaning tank 2# and the picking head alcohol cleaning tank 3# for cyclic alcohol cleaning, the heating unit array is used for carrying out heat source irradiation drying on the picking head, air flows in through the air inlet 1# and the air inlet 2# and is discharged through the air outlet, the flowing air can cool the picking head so as to avoid cross contamination among batches, and meanwhile, after sterilization, disinfection and sterilization liquid needs to be effectively removed, meanwhile, the normal temperature of the picking head is guaranteed after disinfection and sterilization, and the safety of target bacteria/cells is guaranteed, so that in the picking process, the cross contamination among different monoclonal samples is effectively avoided.
Drawings
FIG. 1 is a schematic diagram of an external structure of a multi-channel picking head core unit according to the present invention;
FIG. 2 is a schematic diagram of the internal structure of the multi-channel picking head core unit of the present invention;
FIG. 3 is a schematic diagram of the integration of a single-channel structure with a multi-channel module according to the present invention;
FIG. 4 is a schematic view of the pick head adsorption structure of the present invention;
FIG. 5 is a schematic view of the Z-axis motion assembly of the present invention;
FIG. 6 is a schematic view of a vision and distance sensor assembly according to the present invention;
FIG. 7 is a schematic diagram of a real-time height adjustment process of the present invention;
FIG. 8 is a schematic view of the mounting structure of the multi-channel picker unit of the present invention;
FIG. 9 is a basic flow chart of the present invention for single clone selection;
FIG. 10 is a head picking cleaning array diagram of the monoclonal picking unit of the present invention;
FIG. 11 is a schematic diagram of the internal structure of the picking head cleaning array of the monoclonal picking unit of the present invention;
FIG. 12 is a basic flow chart of the present invention for the cleaning of the pick head.
In the figure: 1. a multi-channel picking head core unit; 2. moving the X-Y linear motor platform; 3. a multi-channel picking head cleaning unit; 101. a unit rectifying cover; 102. a Z-axis motor; 103. a distribution valve block; 104. packaging the gas circuit switch valve group; 105. a pneumatic telescoping assembly; 106. a ram connecting piece; 107. picking a head; 108. a distance sensor; 109. a camera; 110. a support; 301. picking head alcohol cleaning tank 1 #; 302. picking head alcohol cleaning tank 2 #; 303. picking head alcohol cleaning tank 3 #; 304. a ram drying groove; 305. an air inlet 1 #; 306. an air inlet 2 #; 307. an array of warming cells; 308. an air outlet.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, rather than all embodiments, and all other embodiments obtained by a person of ordinary skill in the art without any creative work based on the embodiments of the present invention belong to the protection scope of the present invention.
While several embodiments of the present invention will be described more fully hereinafter with reference to the accompanying drawings, in order to facilitate an understanding of the invention, the invention may be embodied in many different forms and should not be construed as limited to the embodiments set forth herein, but rather should be construed to provide a more complete disclosure of the invention.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs, and the terms used herein in the specification of the present invention are for the purpose of describing particular embodiments only and are not intended to limit the present invention, and the term "and/or" as used herein includes any and all combinations of one or more of the associated listed items.
Referring to fig. 1-12, the present invention provides a technical solution:
a high-throughput single-clone automatic selection module comprises a multi-channel picking head core unit 1, a movable X-Y linear motor platform 2 and a multi-channel picking head cleaning unit 3, wherein the multi-channel picking head core unit 1 comprises a unit rectifying cover shell 101, a Z-axis motor 102, a distribution valve block 103, a packaging air path switch valve group 104, a pneumatic telescopic assembly 105, a picking head connecting piece 106, a picking head 107, a distance sensor 108, a camera 109 and a support 110, and the multi-channel picking head cleaning unit 3 comprises a picking head alcohol cleaning tank 1#301, a picking head alcohol cleaning tank 2#302, a picking head alcohol cleaning tank 3#303, a picking head drying tank 304, an air inlet 1#305, an air inlet 2#306, a heating unit array 307 and an air outlet 308.
Furthermore, the tail end of the picking head 107 is provided with an adsorption groove, the best selecting effect and transfer safety can be achieved, the picking head 107 is prevented from falling in the carrying process, the picking head 107 is made of high-performance stainless steel meeting GMP requirements and is resistant to common sterilizing agents, the guide part of the picking head 107 is made of high-wear-resistant materials and meets FDA (food and drug administration) certification, dust and particulate pollution in the moving process is prevented, the picking head 17 and the driving mechanism are arranged in a floating compensation connection mode, the processing and assembling difficulty can be reduced on the premise that the relative precision of the picking head 107 in multiple channels is guaranteed, the driving unit and the control element of the picking head 107 are designed in a modularized mode, the random flux integration from a single channel to a 96 channel can be flexibly achieved, the picking head 107 in the multiple channels is uniformly provided with hard tail end limiting, the plane formed by one-time processing guarantees the accuracy of the descending distance of the picking head 107, the mounting hole of the multiple channels picking head 107 is formed by one-time processing, the accuracy of the relative position of the picking head 107 in multiple channels is guaranteed, the multi-channel driving part fixing mechanism is designed in a modularized mode, and the integration of the single channel to the 96 channels with any flux can be flexibly achieved.
Furthermore, the Z-axis motor 102 can drive the pick head 107 to realize the movement in the Z-axis direction, and a motor brake device is configured in the Z-axis motor 102, so that self-locking can be realized under the condition of power failure, and the safety in the operation process is ensured.
Furthermore, the camera 109 is a high-resolution visual camera and is provided with a magnifying focusing lens, so that a local magnifying function can be realized for accurately positioning the monoclonal sample, and a specially matched visual recognition algorithm is arranged in the camera 109, so that the colony with the minimum size of 0.05mm can be recognized and positioned.
Further, the distance sensor 108 can use different types of sensors such as laser and ultrasound, preferably a high-precision laser displacement sensor, and can realize the distance identification of the minimum 2um, accurately control the descending height of the picking head 107, and realize the colony selection.
Further, the basic flow of monoclonal selection is as follows: firstly, moving an X-Y linear motor platform 2 to transfer a multichannel picker core unit 1 to a target orifice plate position to finish primary aiming, moving the X-Y linear motor platform 2 to drive the multichannel picker core unit 1 to move, completing flatness scanning of a selected plate and calibrating height distance by a distance sensor 108, simultaneously performing real-time picture shooting by a camera 109 to finish real-time position calculation of target bacteria or cells, controlling a computer to finish selecting sequence scheduling, then selecting the position of the target bacteria or cells, moving the X-Y linear motor platform 2 to an accurate aiming position, quickly confirming the position by the camera, performing micro-motion adjustment by a Z-axis motor 102 to match the local height of the selected position, assembling a single-channel air valve in an air channel switch valve group 104 to move, finishing up-and-down movement of a single channel picker 107 to realize selecting operation, and selecting the next single clone to select a target, the above procedure was repeated until the 96-well plate was finished with elution of the whole plate monoclonal picked product.
Furthermore, after the single clone of each batch is selected, the multichannel picking head core unit 1 needs to be deeply cleaned to avoid cross contamination among batches, and meanwhile, after sterilization, the disinfection and sterilization liquid needs to be effectively removed, and meanwhile, after sterilization and sterilization, the normal temperature of the picking head 107 is guaranteed, and the safety of target bacteria/cells is guaranteed.
Further, the deep cleaning comprises the following specific steps: the picking head 107 is sequentially sent into a picking head alcohol cleaning tank 1#301, a picking head alcohol cleaning tank 2#302 and a picking head alcohol cleaning tank 3#303 for cyclic alcohol cleaning, a heating unit array 307 performs heat source irradiation drying on the picking head, air flows in through an air inlet 1#305 and an air inlet 2#306 and is discharged through an air outlet 308, and the flowing air cools the picking head 107.
Further, the multi-channel picking head core unit 1 may be configured with 1, 2, 4, 8, 16, 24, 48, 96, etc. picking heads with different numbers based on the process requirements, and the multi-channel picking head cleaning unit 3 may be configured with 1, 2 or 3 picking head alcohol cleaning tanks based on the process requirements.
Furthermore, in the process of selecting the single clone, when the picking head 107 moves horizontally, the distance sensor 108 can measure the thickness of the agar coating on the target orifice plate in real time, the Z-axis motor 102 can drive the picking head 107 to move up and down according to the measuring mechanism of the distance sensor 108, and the distance from the picking head 107 to the agar coating on the target orifice plate can be kept constant.
The working process of the invention is as follows: firstly, moving an X-Y linear motor platform 2 to transfer a multichannel picker core unit 1 to a target orifice plate position to finish primary aiming, moving the X-Y linear motor platform 2 to drive the multichannel picker core unit 1 to move, completing flatness scanning of a selected plate and calibrating height distance by a distance sensor 108, simultaneously performing real-time picture shooting by a camera 109 to finish real-time position calculation of target bacteria or cells, controlling a computer to finish selecting sequence scheduling, then selecting the position of the target bacteria or cells, moving the X-Y linear motor platform 2 to an accurate aiming position, quickly confirming the position by the camera, performing micro-motion adjustment by a Z-axis motor 102 to match the local height of the selected position, assembling a single-channel air valve in an air channel switch valve group 104 to move, finishing up-and-down movement of a single channel picker 107 to realize selecting operation, and selecting the next single clone to select a target, repeating the operation of the previous step until the elution of the monoclonal picked product of the whole plate is completed by a 96-well plate;
after the single clone selecting operation is finished, the picking head 107 is sequentially sent into the picking head alcohol cleaning tank 1#301, the picking head alcohol cleaning tank 2#302 and the picking head alcohol cleaning tank 3#303 for circular alcohol cleaning, the heating unit array 307 performs heat source irradiation drying on the picking head, air flows in through the air inlet 1#305 and the air inlet 2#306 and is discharged through the air outlet 308, and the flowing air cools the picking head 107.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims (10)

1. The utility model provides a module is selected in automatic of high flux monoclonal, includes that multichannel is chosen first core unit (1), removes X-Y linear electric motor platform (2) and multichannel is chosen first cleaning unit (3), its characterized in that: the multichannel head picking core unit (1) comprises a unit rectifying cover shell (101), a Z-axis motor (102), a distribution valve block (103), a container air channel switch valve group (104), a pneumatic telescopic assembly (105), a head picking connecting piece (106), a picking head (107), a distance sensor (108), a camera (109) and a support (110), and the multichannel head picking cleaning unit (3) comprises a head picking alcohol cleaning tank 1# (301), a head picking alcohol cleaning tank 2# (302), a head picking alcohol cleaning tank 3# (303), a head picking drying tank (304), an air inlet 1# (305), an air inlet 2# (306), a heating unit array (307) and an air outlet (308).
2. The high throughput monoclonal automated selection module of claim 1, wherein: the tail end of the picking head (107) is provided with an adsorption groove, the best selecting effect and transfer safety can be achieved, the picking head (107) is prevented from falling in the carrying process, the high-performance stainless steel meeting GMP requirements is used for the picking head (107), common sterilizing agents are tolerated, the guide part of the picking head (107) is made of high-wear-resistant materials, FDA certification is met, dust and particulate pollution in the moving process is prevented, the picking head (17) and the driving mechanism are connected through floating compensation, the processing and assembling difficulty can be reduced on the premise that the relative precision of the picking head (107) in multiple channels is guaranteed, the driving unit and the control element of the picking head (107) are in a modularized design, the integration of the random flux from the single channel to the 96 channels can be flexibly realized, the use of the picking head (107) in the multiple channels is unified, the tail end is hard limited, and the precision of the descending distance of the picking head (107) is guaranteed through a one-step processing molded plane, the mounting hole of multichannel pickhead (107), one shot forming guarantees the accuracy of picking head (107) relative position in the multichannel, and multichannel drive part fixed establishment uses the modularized design, can realize in a flexible way that the single channel is integrated to 96 arbitrary fluxes of passageway.
3. The high throughput monoclonal automated selection module of claim 1, wherein: the Z-axis motor (102) can drive the picking head (107) to move in the Z-axis direction, and a motor brake device is arranged in the Z-axis motor (102), so that self-locking can be realized under the condition of power failure, and the safety in the operation process is guaranteed.
4. The high throughput monoclonal automated selection module of claim 1, wherein: the video camera (109) is a high-resolution visual camera and is provided with a magnifying focusing lens, a local magnifying function can be realized to be used for accurately positioning the monoclonal sample, and a specially matched visual recognition algorithm is arranged in the video camera (109), so that the colony with the minimum size of 0.05mm can be recognized and positioned.
5. The high throughput monoclonal automated selection module of claim 1, wherein: the distance sensor (108) can use different types of sensors such as laser and ultrasound, preferably a high-precision laser displacement sensor, can realize the distance identification of the minimum 2um, accurately controls the descending height of the picking head (107), and realizes the colony selection.
6. The high throughput monoclonal automated selection module of claim 1, wherein: the basic process of monoclonal selection is as follows: firstly, an X-Y linear motor platform (2) is moved to transfer a multi-channel picker core unit (1) to a target hole plate position to finish primary aiming, the X-Y linear motor platform (2) is moved to drive the multi-channel picker core unit (1) to move, a distance sensor (108) finishes planeness scanning of a selected plate and calibrates a height distance, a camera (109) carries out real-time picture shooting at the same time to finish real-time position calculation of target bacteria or cells, a computer is controlled to finish selection sequence scheduling, then the position of the target bacteria or cells is selected, the X-Y linear motor platform (2) is moved to an accurate aiming position, the camera quickly confirms the position, a Z-axis motor (102) carries out micro-adjustment, the local height of the selected position is matched, a single-channel air valve in an air channel packaging switch valve group (104) moves to finish up-and-down movement of a single-channel picker (107), and (4) realizing the selection operation, selecting the next monoclonal selection target, and repeating the operation of the previous step until the elution of the whole plate of monoclonal selection products is completed by the 96-well plate.
7. The high throughput monoclonal automated selection module of claim 1, wherein: after the single clone of each batch is selected, the multichannel picking head core unit (1) needs to be deeply cleaned so as to avoid cross contamination among batches, and meanwhile, after sterilization, the multichannel picking head core unit needs to effectively remove sterilization liquid, and meanwhile, after sterilization, the normal temperature of the picking head (107) is guaranteed, and the safety of target bacteria/cells is guaranteed.
8. The high throughput monoclonal automated selection module of claim 1, wherein: the deep cleaning method comprises the following specific steps: the picking head (107) is sequentially sent into a picking head alcohol cleaning tank 1# (301), a picking head alcohol cleaning tank 2# (302) and a picking head alcohol cleaning tank 3# (303) for cyclic alcohol cleaning, a heating unit array (307) performs heat source irradiation drying on the picking head, air flows in through an air inlet 1# (305) and an air inlet 2# (306) and is discharged through an air outlet (308), and the flowing air can cool the picking head (107).
9. The high throughput monoclonal automated selection module of claim 1, wherein: the multichannel picking head core unit (1) can be configured with 1, 2, 4, 8, 16, 24, 48, 96 and other picking heads with different numbers based on process requirements, and the multichannel picking head cleaning unit (3) can be configured with 1, 2 or 3 picking head alcohol cleaning tanks based on process requirements.
10. The high throughput monoclonal automated picking module of claim 6, wherein: in the process of monoclonal selection, when the picking head (107) moves horizontally, the distance sensor (108) can measure the thickness of the agar coating on the target orifice plate in real time, the Z-axis motor (102) can drive the picking head (107) to move up and down according to a measuring mechanism of the distance sensor (108), and the distance from the picking head (107) to the agar coating on the target orifice plate can be kept constant.
CN202111412929.1A 2021-11-25 2021-11-25 High-flux monoclonal automatic selection module Pending CN114015554A (en)

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