CN105132279A - Probe 96 for selecting bacterial colony in high-flux mode - Google Patents
Probe 96 for selecting bacterial colony in high-flux mode Download PDFInfo
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- CN105132279A CN105132279A CN201510643365.0A CN201510643365A CN105132279A CN 105132279 A CN105132279 A CN 105132279A CN 201510643365 A CN201510643365 A CN 201510643365A CN 105132279 A CN105132279 A CN 105132279A
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- piston rod
- main body
- magnetic valve
- pin
- longitudinal
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
- C12M33/06—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles for multiple inoculation or multiple collection of samples
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
Abstract
The invention relates to a probe 96 for selecting a bacterial colony in a high-flux mode. The probe 96 is composed of an upper body and a lower body. Ninety-six vertical piston rod passageways in an array with 8 lines in the longitudinal direction and 12 lines in the transverse direction are distributed in the upper body. A piston rod is arranged in each piston rod passageway, and therefore an upper sealed cavity and a lower sealed cavity are formed in each piton rod passageway. The 8 longitudinal upper sealed cavities in each longitudinal line are communicated with one another, connected with first electromagnetic valves through transverse air inlets and used for controlling the opening and closing of the transverse air inlets. The 12 transverse lower sealed cavities in each transverse line are communicated with one another, connected with second electromagnetic valves through longitudinal air inlets and used for controlling the opening and closing of the longitudinal air inlets. Ninety-six selecting needle passageways arrayed corresponding to the piston rod passageways in the upper body are distributed in the lower body. A selecting needle is arranged in each selecting needle passageway. A reset spring is arranged between each selecting needle passageway and the corresponding selecting needle. The first electromagnetic valves and the second electromagnetic valves are connected with an air pump. The probe 96 is used in cooperation with an operation tail end of an automatic machine or robot for selecting the bacterial colony, and the bacterial colony is selected automatically.
Description
Technical field
The present invention relates to a kind of bio-science instrument, especially a kind of high-throughput device for sorting bacterium colony being selected orifice plate from culture dish, for automatization colony picker, realize selecting and shifting of bacterium colony.
Background technology
Quality strains is differentiated by the morphological feature of bacterium colony and is selected, and traditional bacterium colony is selected mode and relied on the features such as the form color of naked eyes identification bacterium colony, uses inoculating needle to select by manual type.This to select the professional requirement of mode to operator higher, and hand picking work is very loaded down with trivial details, repeatability and efficiency all very low, omnidistance precision cannot be ensured.In PRODUCTION TRAITS, bacterium colony selected sample substantial amounts, hand picking complex procedures, length consuming time, efficiency are low.
Therefore, need the automated installation of bacterium colony identification and screening process, automatic bacterial colony selects instrument can improve precision and the efficiency that bacterium colony selects work greatly, meets the needs of production.
Chinese invention patent (201120335923.4) and utility model patent (201110265054.7) propose a kind of pick-up head of Automated bacterial colony sorting apparatus, a depression bar is driven with three axial filament bars, complete by next root pin and select, this plant automation degree is extremely low, cannot complete a large amount of to select work.
Chinese utility model patent (200420007315.0) proposes a kind of clone's picking head for biological gene engineering, and add spool in employing movement and complete ventilation and die, this device for mechanical structure is too complicated, and difficulty of processing is large, and in chamber, stopping property is difficult to ensure card.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of 96 probes selected for high-throughput bacterium colony being used in automatic bacterial colony selection equipment, to realize selecting and shifting of bacterium colony.
For achieving the above object, technical scheme of the present invention is: a kind of 96 probes selected for high-throughput bacterium colony, be made up of upper main body and lower main body, longitudinal 8 are furnished with in described upper main body, laterally 96 vertical piston rod passages of 12 arrangements, piston rod passage is built with piston rod, piston rod passage is formed, lower seal cavity, often longitudinal 8 the upper sealed cavities of row are interconnected, and connect the first magnetic valve by horizontal air inlet port, ventilate in horizontal air inlet port for controlling and die, often horizontal 12 the lower seal cavitys of row are interconnected, and connect the second magnetic valve by longitudinal air inlet port, ventilate in longitudinal air inlet port for controlling and die, be furnished with in described lower main body and select needle passageway with 96 of the corresponding arrangement of piston rod passage in upper main body, select needle passageway built with selecting pin, select needle passageway and select between pin and return spring is housed, described first magnetic valve is connected air pump with the second magnetic valve.
During original state, described return spring holds out against selects pin, tightly selects pin top and is located by the lower main body pressing plate be fixed on lower main body upper surface, make 96 to select pin and be contracted in same position.
On the piston of described piston rod, cover has O type circle, and be slidably connected by O type circle and piston rod channel seal, described piston rod channel bottom to be tightly connected piston rod by O type circle, seal cap is provided with in piston rod channel upper, seal cap is connected with piston rod channel seal by O type circle, and seal cap upper end is compressed by the upper main body top pressing board be fixed on upper main body upper surface.
Described first magnetic valve and the second magnetic valve are two-position three way magnetic valve.
The invention has the beneficial effects as follows: bacterium colony of the present invention selects 96 probes can stretching with 20 solenoid control, 96 pins.Wherein, upper main body and lower main body can be dismantled easily, be installed.This device needs outside connection one air-source air feeding device, and the induction trunk in logical upper main body enters in piston rod channel lumens, by the switch that magnetic valve is orderly, make the high atmospheric pressure produced in sealed environment, piston rod is stretched, drives in lower main body simultaneously and select the flexible of pin, complete the process selected.Upper and lower body portion from design conveniently can select cleaning and the replacing of pin.This high-throughput bacterium colony selects the operational tip that 96 probes are used in automaticmachines that bacterium colony selects, robot, can realize the automatization that high-throughput bacterium colony is selected easily, rapidly and efficiently complete the work selected, shift, realize automatization bacterium colony and select.
Accompanying drawing explanation
Fig. 1 is the structural upright schematic diagram of 96 probes selected for high-throughput bacterium colony of the present invention;
Fig. 2 is the structure partial sectional view of 96 probes selected for high-throughput bacterium colony of the present invention;
Fig. 3 is the structure right view of 96 probes selected for high-throughput bacterium colony of the present invention;
Fig. 4 is the structure longitudinal sectional view of 96 probes selected for high-throughput bacterium colony of the present invention;
Fig. 5 is upper agent structure and inner chamber part sectioned view;
Fig. 6 loads piston rod schematic diagram in upper main body;
Fig. 7 is lower agent structure schematic perspective view;
Fig. 8 is lower agent structure sectional view.
Embodiment
Below in conjunction with accompanying drawing and embodiment, the present invention is further illustrated.
As shown in Figures 1 to 8, for 96 probes that high-throughput bacterium colony is selected, mainly comprise: go up main body 1, lower main body 2, piston rod 3, O type circle 4, select pin 5, return spring 6, first, second magnetic valve 7a, 7b, first and second inlet suction port 8a, 8d, first and second exhaust joint 8b, 8c, air inlet port 9a, venting hole 9b, piston rod path 10, longitudinal air inlet port 11, horizontal air inlet port 12, nut 13, upper main body top pressing board 14, upper main body base pressure plate 15, seal cap 16, lower main body pressing plate 17, screw 18, blend stop 19, select needle passageway 20.
Bacterium colony of the present invention selects 96 probes can stretching with 20 solenoid control, 96 pins.Wherein, upper main body and lower main body can be dismantled easily, be installed.
For 96 probes that high-throughput bacterium colony is selected, be made up of upper main body and lower main body two portions, use air pump air feed.Upper main body has 96 piston rod path 10s, and each piston rod path 10 is built with piston rod 3, and when air pressure is arranged at piston rod 3 top, piston rod 3 moves downward.Lower main body 96 selects needle passageway 20 built with selecting pin 5, when upper main body piston rod 3 moves downward, ejects lower main body and selects pin 5, when having air pressure in piston rod 3 bottom, piston rod moves upward, and the pin 5 of selecting of lower main body is regained under spring restoring force effect, completes similar artificial picking.In a set of probe, only need a upper main body, selecting pin in lower main body has different size, can with changing different lower main bodys when selecting different strains.
As Fig. 5, shown in 6, upper main body has longitudinally 8 x laterally 12 piston rod path 10s arranged, piston rod path 10 inwall is very smooth, piston rod path 10 is built with piston rod 3, piston rod path 10 is formed, lower seal cavity, often longitudinal 8 the upper sealed cavities of row are interconnected, and connect the first magnetic valve 7a by horizontal air inlet port 12, ventilate in horizontal air inlet port 12 for controlling and die, often horizontal 12 the lower seal cavitys of row are interconnected, and connect the second magnetic valve 7b by longitudinal air inlet port 11, ventilate in longitudinal air inlet port 11 for controlling and die.Top has horizontal air inlet port 12, and make piston rod 3 top produce high atmospheric pressure, beneath galleries has longitudinal air inlet port 11, makes piston rod 3 bottom produce high atmospheric pressure, and the turnover gas controlling top and bottom just can the moving up and down of control piston bar.
Upper main body is equipped with magnetic valve and is used for the turnover gas of control piston bar top and the bottom.Magnetic valve adopts two-position three way magnetic valve, is used for controlling air inlet and exhaust.The regular opening and closing of magnetic valve can control whole flexible of flexible, 96 pins of any pin, also can control the flexible of pin in a certain matrix area.
The sealing of O type circle is all equipped with in upper main body piston rod path 10 top and bottom, and ensure there is stopping property in passage, on piston rod 3, cover has O type circle, can move up and down when there being air pressure.
On piston rod 3, cover has O type circle 4 to put into piston rod path 10, and piston rod path 10 top uses seal cap 16 to overlap O type circle 4 and seals, and use main body top to press version press seal cap, seal cap 16 plays spacing effect simultaneously.With the sealing of O type circle bottom piston rod path 10, make to form sealed environment in piston rod channel lumens.
As Fig. 7, shown in 8, being furnished with in lower main body and selecting needle passageway 20 with 96 of the corresponding arrangement of piston rod path 10 in upper main body, selecting needle passageway 20 built with selecting pin 5, select needle passageway 20 and select between pin 5 and return spring 6 is housed.
During original state, return spring 6 holds out against selects pin 5, tightly selects pin 5 top and is located by the lower main body pressing plate 17 be fixed on lower main body upper surface, make 96 to select pin 5 and be contracted in same position.
Be equipped with in lower main body and select pin and return spring, select stretching out of pin and rely on ejecting of upper main body piston rod, shortening relies on the restorer of spring.
When external air source enters in air inlet port 9a by the first inlet suction port 8a, enter the first magnetic valve 7a of the air in air inlet port 9a by two-position three way, after first magnetic valve 7a powers on, in air inlet port 9a, air enters horizontal air inlet port 12, if the second magnetic valve 7b closes, then piston rod top air pressure is greater than underpart of the piston rod air pressure, and piston rod stretches out downwards fast.Otherwise then piston rod upwards fast retractile.
Piston rod path 10 and select needle passageway 20 one_to_one corresponding when upper main body and lower main body are installed, when Controlling solenoid valve switch, piston rod 3 stretches out under gas pressure, and piston rod 3 ejects selects pin 5.When piston rod 3 is retracted, select pin 5 and retract under the effect of return spring 6, return to original state, complete the process selected.
The switch of the first magnetic valve 7a in upper main body can to control in horizontal air inlet port 12 ventilation and die, and the second magnetic valve 7b can to control in longitudinal air inlet port 11 ventilation and die, when regular switch electromagnetic valve just can the stretching of control piston bar 3.When whole first magnetic valve 7a closes, whole second magnetic valve 7b opens, and selects pin 5 and is in whole retracted mode; When whole first magnetic valve 7a opens, whole second magnetic valve 7b closes, and selects pin 5 and is in and all stretches out state; When only having certain a line to open in the first magnetic valve 7a, when only having a certain row to close in the second magnetic valve 7b, select pin 5 and stretch out for one of ranks intersection, the first magnetic valve electromagnetism 7a closes, and the first magnetic valve 7a opens, and selects pin 5 and retracts.The switch of the continuous a few row of Controlling solenoid valve and several row just can control to select the flexible of pin 5 in a matrix area.
Claims (4)
1. 96 probes selected for high-throughput bacterium colony, be made up of upper main body and lower main body, it is characterized in that: in described upper main body, be furnished with longitudinal 8, laterally 96 vertical piston rod passages (10) of 12 arrangements, piston rod passage (10) is built with piston rod (3), make in piston rod passage (10) formation, lower seal cavity, often longitudinal 8 the upper sealed cavities of row are interconnected, and connect the first magnetic valve (7a) by horizontal air inlet port (12), ventilate in horizontal air inlet port (12) for controlling and die, often horizontal 12 the lower seal cavitys of row are interconnected, and connect the second magnetic valve (7b) by longitudinal air inlet port (11), ventilate in longitudinal air inlet port (11) for controlling and die, be furnished with in described lower main body and select needle passageway (20) with 96 of piston rod passage (10) the corresponding arrangement in upper main body, select needle passageway (20) built with selecting pin (5), select needle passageway (20) and select between pin (5) and return spring (6) is housed, described first magnetic valve (7a) is connected air pump with the second magnetic valve (7b).
2. 96 probes selected for high-throughput bacterium colony according to claim 1, it is characterized in that: during original state, described return spring (6) holds out against selects pin (5), tightly select pin (5) top to be located by the lower main body pressing plate (17) be fixed on lower main body upper surface, make 96 to select pin (5) and be contracted in same position.
3. 96 probes selected for high-throughput bacterium colony according to claim 1, it is characterized in that: on the piston of described piston rod (3), cover has O type circle (4), and sealed by O type circle (4) and piston rod passage (10) and be slidably connected, described piston rod passage (10) bottom to be tightly connected piston rod (3) by O type circle, seal cap (16) is provided with in piston rod passage (10) top, seal cap (16) is tightly connected by O type circle (4) and piston rod passage (10), seal cap (16) upper end is compressed by the upper main body top pressing board (14) be fixed on upper main body upper surface.
4. 96 probes selected for high-throughput bacterium colony according to claim 1, is characterized in that: described first magnetic valve (7a) and the second magnetic valve (7b) are two-position three way magnetic valve.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510643365.0A CN105132279B (en) | 2015-10-08 | 2015-10-08 | 96 probes selected for high flux bacterium colony |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510643365.0A CN105132279B (en) | 2015-10-08 | 2015-10-08 | 96 probes selected for high flux bacterium colony |
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| Publication Number | Publication Date |
|---|---|
| CN105132279A true CN105132279A (en) | 2015-12-09 |
| CN105132279B CN105132279B (en) | 2017-08-25 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510643365.0A Active CN105132279B (en) | 2015-10-08 | 2015-10-08 | 96 probes selected for high flux bacterium colony |
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| Country | Link |
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| CN (1) | CN105132279B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108441417A (en) * | 2018-05-31 | 2018-08-24 | 深圳先进技术研究院 | Bacterial clump picking instrument |
| CN109321429A (en) * | 2018-09-28 | 2019-02-12 | 农业部沼气科学研究所 | A kind of automatical bacterium instrument and microbe to screen method |
| CN113201446A (en) * | 2021-05-10 | 2021-08-03 | 北京化工大学 | Automatic bacterial colony of high flux picks device |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3650306A (en) * | 1970-09-18 | 1972-03-21 | Cooke Eng Co | Laboratory dispensing apparatus |
| US6374683B1 (en) * | 1999-01-29 | 2002-04-23 | Genomic Instrumentation Services, Inc. | Pipetter |
| CN2706476Y (en) * | 2004-03-16 | 2005-06-29 | 北京机械工业自动化研究所 | Clone pricking head for biological gene engineering |
| CN201263982Y (en) * | 2008-09-28 | 2009-07-01 | 王学东 | Piston type quantitative liquid injection washing head |
-
2015
- 2015-10-08 CN CN201510643365.0A patent/CN105132279B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3650306A (en) * | 1970-09-18 | 1972-03-21 | Cooke Eng Co | Laboratory dispensing apparatus |
| US6374683B1 (en) * | 1999-01-29 | 2002-04-23 | Genomic Instrumentation Services, Inc. | Pipetter |
| CN2706476Y (en) * | 2004-03-16 | 2005-06-29 | 北京机械工业自动化研究所 | Clone pricking head for biological gene engineering |
| CN201263982Y (en) * | 2008-09-28 | 2009-07-01 | 王学东 | Piston type quantitative liquid injection washing head |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108441417A (en) * | 2018-05-31 | 2018-08-24 | 深圳先进技术研究院 | Bacterial clump picking instrument |
| CN108441417B (en) * | 2018-05-31 | 2024-01-16 | 深圳先进技术研究院 | Bacterial colony picking instrument |
| CN109321429A (en) * | 2018-09-28 | 2019-02-12 | 农业部沼气科学研究所 | A kind of automatical bacterium instrument and microbe to screen method |
| CN109321429B (en) * | 2018-09-28 | 2020-09-08 | 农业部沼气科学研究所 | Automatic bacterium picking instrument and microorganism screening method |
| CN113201446A (en) * | 2021-05-10 | 2021-08-03 | 北京化工大学 | Automatic bacterial colony of high flux picks device |
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| Publication number | Publication date |
|---|---|
| CN105132279B (en) | 2017-08-25 |
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