CN114009618A - Feed additive for preventing intestinal barrier injury of piglets and preparation method and application thereof - Google Patents
Feed additive for preventing intestinal barrier injury of piglets and preparation method and application thereof Download PDFInfo
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Abstract
The invention provides a feed additive for preventing intestinal barrier injury of piglets, a preparation method and application thereof, wherein the feed additive consists of active components and a carrier matrix; the active component is prepared from the following raw materials in parts by weight: 200 parts of taurine 150-. The feed additive can be added into daily ration for piglet to prepare piglet feed. The components of the feed additive are synergistic and mutually promoted, so that the influence of LPS on the transcellular resistance of intestinal mucosal epithelial cells of piglets can be remarkably prevented, the expression level of intestinal mucosal epithelial cell tight junction protein is improved, the mechanical barrier of the intestinal mucosal epithelial cells of the piglets is protected, the intestinal mucosal permeability is reduced, the daily gain is improved, and the feed conversion ratio is reduced.
Description
Technical Field
The invention relates to the technical field of feed additives, in particular to a feed additive for preventing intestinal barrier injury of piglets and a preparation method and application thereof.
Background
The intestinal tract is not only the main place for absorbing nutrient substances, but also an important defense line for protecting the organism from pathogenic bacteria, and the normal development of the intestinal tract is very important for the health of piglets. The intestinal tract mechanical barrier is a barrier which is formed by intestinal mucosa epithelial cells, tight connection of the intestinal mucosa epithelial cells and the like and isolates a body from an external environment. Piglet diarrhea caused by the damage of piglet intestinal barrier caused by various reasons is one of the most main piglet diseases and is also an important cause of piglet death. According to survey statistics, the average incidence rate of diarrhea caused by various reasons of piglets below 25kg accounts for about 50%, and the mortality rate caused by the diarrhea accounts for more than 40% of the mortality rate of the total piglets. Therefore, the treatment effect of piglet diarrhea is not ideal due to the problems of poor tolerance, drug resistance and the like of piglets. Therefore, it is more meaningful to take effective measures to prevent the disease than to take post-disease treatment.
LPS (1 ipolysochiccharide), a lipopolysaccharide, also known as endotoxin (endoxin), is a major factor in gram-negative bacteria causing innate immune responses. The series of inflammatory cells (including intestinal mucosal epithelial cells) of the body which can be triggered by the strain take part in inflammatory mediator release linkage reaction, so that 'inflammatory factor storm' is triggered, the intestinal mucosal epithelial cells are damaged, and piglet diarrhea is caused. The specific process is as follows: LPS in plasma will first bind to LPS-binding protein synthesized by the Liver (LBP), which presents LPS to immune cell surface CDl4, but CDl4 does not have an intracellular structure to signal, and CDl4 further presents LPS to the TLR4/MD2 complex, which signals via its downstream MyD 88-dependent and independent pathways. Wherein, the MyD 88-dependent signal pathway comprises NF-kB and MAPK two pathways, and the activation of the pathway leads to the release of pro-inflammatory factors, such as IL-1 beta, IL-6 and TNF-alpha; whereas MyD88 independent pathway activation leads to secretion of IFN- β and like mediators. The inflammatory factors further damage intestinal mucosa epithelial cells, destroy a tight connection structure, increase intestinal mucosa permeability and cause piglet diarrhea.
Piglet diarrhea caused by gram-negative bacterial infection has been mainly treated by antibiotics, but a large number of gram-negative bacteria are killed by the antibiotic treatment, which accelerates the release of endotoxin (LPS) into blood and rather induces systemic sepsis, and thus, research on endotoxin antagonistic measures such as bacterial endotoxin synthesis inhibitors, endotoxin-related receptor antagonists, and the like has never been terminated. Although methods for antagonizing LPS have been studied for many years, none of the compounds, as small as artificially synthesized, and as large as antibody proteins, has been really applied to production.
Aiming at the problems of antibiotic abuse, drug residues and the like, Philip in 2003 proposes the concept of nutritional immunity, namely, the regulation of body functions, inflammation and animal immunity through nutrients. Therefore, based on the concept, the taurine compound feed additive for preventing the damage of LPS to the intestinal mucosa is developed, and the technology for improving the damage of the intestinal mucosa barrier caused by LPS by a nutrition regulation and control means has important significance for the intestinal health of the piglets.
Disclosure of Invention
Aiming at the technical problems that piglets are easy to be damaged by immune stress caused by various reasons, cause diarrhea of the piglets and seriously influence the development of the domestic pig breeding industry, the invention mainly aims to provide the feed additive which takes taurine as the main component and can effectively prevent the damage of the intestinal barriers of the piglets and the diarrhea of the piglets, and the preparation method and the application thereof.
In order to achieve the above object, the technical solution of the present invention is as follows.
A feed additive for preventing intestinal barrier injury of piglets comprises active components and carrier matrix; the active component is prepared from the following raw materials in parts by weight:
200 parts of taurine 150-.
Further, the carrier matrix is any one of or a mixture of at least two of bran meal, rice bran, unite bran, corncob powder, corn gluten meal, zeolite powder and bentonite.
Further, the mass ratio of the active component to the carrier matrix is 350-640: 150-350.
Further, the mass ratio of the vitamin A to the vitamin C to the folic acid is 1: 1: 1.
a preparation method of a feed additive for preventing intestinal barrier injury of piglets comprises the following steps:
s1, weighing the raw materials according to the weight part ratio of the feed additive;
s2, uniformly mixing a part of carrier matrix with vitamin A, vitamin C and folic acid to obtain a mixture A;
s3, uniformly mixing the rest carrier matrix with taurine, arginine, glutamine, sodium butyrate and zinc oxide to obtain a mixture B;
s4, uniformly mixing the mixture A of the S2 and the mixture B of the S3 to prepare the feed additive for preventing the intestinal barrier damage of the piglets.
Further, the total amount of the active components is 350-640 parts by weight; the total amount of the carrier matrix is 150-350 parts by weight.
The feed additive for preventing the piglet intestinal barrier injury is applied to being added into piglet daily ration to prepare piglet feed.
Further, the dosage of the feed additive for preventing the intestinal barrier injury of the piglets accounts for 1-2% of the total mass of the piglet feed.
In the invention, the taurine is a conditionally essential amino acid, and researches show that the taurine is not necessary in a healthy state, but can maintain or restore the integrity of the intestinal structure and function of piglets in certain stress or disease states, and the taurine is specifically characterized in that the taurine can be combined with LPS, inhibit the combination of LPS and TLR4, inhibit the release of proinflammatory factors (TNF-alpha, IL-6, IL-1 beta and IFN-beta), promote the release of anti-inflammatory factors (IL-4 and IL-10), improve the expression level of occludin, claudin-1 and zonulin-1 proteins in the jejunal mucosa, reduce the lactic acid level and diamine oxidase (DAO) activity in blood plasma, improve the daily gain and reduce the feed-meat ratio.
The arginine can induce the growth of intestinal mucosa of piglets, improve the villus height of small intestine in intestinal tract and reduce the depth of crypt. Effectively preventing the intestinal tight junction damage of piglets caused by ischemia, changing the transmembrane resistance value of the intestinal tract and reducing the permeability of the intestinal mucosa. The arginine feeding can also obviously improve the level of intestinal secretory IgA and IL-10 and reduce the displacement of intestinal bacteria.
The glutamine can obviously increase the weight, DNA and RNA content of the intestinal mucosa of a rat, recover the height of villus, the surface area and the depth of crypts, increase the mitosis of crypt cells, accelerate the renewal speed of the intestinal epithelial cells, enhance the repair capacity, enhance the tight connection among the intestinal mucosa cells, reduce the apoptosis of the epithelial cells, prevent the atrophy of the intestinal mucosa and the increase of the permeability caused by inflammation, thereby recovering and maintaining the integrity of the morphology and the function of the mucosa.
The sodium butyrate has special rancidity-like odor of cheese and has a certain food calling effect on piglets, and meanwhile, the sodium butyrate contains short-chain volatile fatty acid butyric acid, so that generated butyrate root ions can improve the stress resistance of the piglets; the sodium butyrate is added to provide nutrition for intestinal mucosa of the piglets, promote the repair and growth of intestinal villus structures, enhance the intestinal immunity, inhibit harmful bacteria, promote the proliferation of beneficial bacteria, improve the intestinal environment and improve the palatability of the feed.
The zinc oxide can adsorb and adhere to the surface of the intestinal tract to form a protective biological film so as to reduce the damage of the antigen to the intestinal tract; the osmotic pressure of cells in the intestinal tract is maintained to reduce the secretion of intestinal tract ions, so that the water is not lost, the astringency is realized, and the attachment and the absorption of pathogenic bacteria in the intestinal tract are reduced; improve and maintain the stability and diversity of the intestinal flora. Can obviously reduce the diarrhea rate of weaned piglets, increase the daily gain and obviously improve the intestinal mucosa shape.
The invention also utilizes the combination of the vitamin A, the vitamin C and the folic acid, can greatly improve the anti-stress and anti-oxidation capability of the piglets, improve the immunity and effectively promote the intestinal health of the piglets.
Compared with the prior art, the feed additive provided by the invention takes active substance components consisting of taurine, arginine, glutamine, sodium butyrate, zinc oxide, vitamin A, vitamin C and folic acid as nutritional immune additive components according to the influence of damage of intestinal mucosa barrier and piglet diarrhea caused by immune stress which is easily caused by various reasons of piglets, and the components are synergistic and mutually promoted, so that the influence of LPS on the transcellular resistance (TEER) of intestinal mucosa epithelial cells of piglets can be obviously prevented, the expression level of intestinal mucosa epithelial cell tight junction protein is improved, the mechanical barrier of the intestinal mucosa epithelial cells of piglets is protected, the intestinal mucosa permeability is reduced, the daily gain is improved, and the feed conversion ratio is reduced. Meanwhile, the feed additive disclosed by the invention is easy to obtain raw material components, simple in preparation method, low in economic cost and good in application prospect.
The invention has the beneficial effects that:
1. the invention complements the advantages of small molecular compounds beneficial to intestinal health, develops the feed additive mainly containing taurine and has application in improving the intestinal mucosa injury of piglets. Through experimental research, the feed additive disclosed by the invention can inhibit the combination of LPS and TLR4 by combining gram-negative bacterium endotoxin (lipopolysaccharide, LPS), further inhibit the release of proinflammatory factors (TNF-alpha, IL-6, IL-1 beta and IFN-beta) by inhibiting the MyD88 dependent pathway and the MyD88 independent pathway, promote the release of anti-inflammatory factors (IL-4 and IL-10) and improve the adverse effect of LPS-induced inflammatory factor storm on intestinal mucosa damage. The method has the advantages of preventing the influence of LPS on the transcellular resistance (TEER) of intestinal mucosal epithelial cells of piglets, improving the expression level of intestinal mucosal epithelial cell tight junction protein, protecting the mechanical barrier of the intestinal mucosal epithelial cells of the piglets, reducing the permeability of intestinal mucosa, improving daily gain and reducing the feed conversion ratio. The technology for improving the intestinal mucosa barrier damage caused by the LPS by the nutrition regulation and control means has important significance for the intestinal health of the piglets, provides reference and technical theoretical basis for improving the intestinal health and production performance of the piglets, has the advantages of convenience and safety in use, no toxic or side effect and the like, and has wide popularization and application prospects.
2. The feed additive can obviously improve the villus length of duodenum, jejunum and ileum of piglets, reduce the depth of crypt and improve the ratio of villus to crypt.
3. The feed additive can obviously protect intestinal mucosa from being damaged by LPS (lipopolysaccharide) on intestinal mucosa epithelial cells.
4. The feed additive can obviously increase the gene expression level of the zon-1, Claudin-1 and Claudin of duodenum, jejunum and ileum.
5. The feed additive can obviously reduce the level of lactic acid (D-LA) and the activity of diamine oxidase (DAO) in blood.
6. The feed additive can obviously reduce the levels of proinflammatory factors (TNF-alpha, IL-6, IL-1 beta and IFN-beta) in intestinal mucosa and improve the levels of anti-inflammatory factors (IL-4 and IL-10).
7. The feed additive provided by the invention can obviously reduce the diarrhea rate of piglets, improve the daily gain of piglets and reduce the feed conversion ratio.
8. The feed additive of the invention is capable of inhibiting its binding to TLR4 by binding LPS.
9. The feed additive is mainly used for being added into feed for weaned pigs, and can protect the damage of the intestinal mucosa mechanical barrier of the piglets, thereby preventing the occurrence of diarrhea of the piglets.
Drawings
FIG. 1 is a comparative graph of paraffin sections observing the effect of the feed additive of example 3 on villus length, crypt depth, ratio of villus length/crypt depth in the duodenum, jejunum, ileum.
FIG. 2 is a comparative scanning electron microscope image showing the effect of the feed supplement of example 3 on the ultrastructure of colonic intestinal mucosal epithelial cells.
In fig. 1 and 2, C represents a control group; LPS means LPS treated group; LPST represents the test group.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The materials and equipment described in the examples of the present invention can be purchased on the market without specific description.
Example 1
A feed additive for preventing intestinal barrier injury of piglets comprises 640 parts of active components and 350 parts of carrier matrix in parts by weight.
The active component is prepared from the following raw materials in parts by weight: 200 parts of taurine, 80 parts of arginine, 80 parts of glutamine, 80 parts of sodium butyrate, 50 parts of zinc oxide, 50 parts of vitamin A, 50 parts of vitamin C and 50 parts of folic acid.
The carrier matrix is prepared from 50% of corncob powder and 50% of corn protein powder by mass percentage.
The preparation method comprises the following steps:
s1, weighing the raw materials according to the weight part ratio of the feed additive;
s2, adding vitamin A, vitamin C and folic acid into 100 parts of carrier matrix, and stirring and mixing uniformly to obtain a mixture A;
s3, adding taurine, arginine, glutamine, sodium butyrate and zinc oxide into the residual carrier substrate, and uniformly stirring and mixing to obtain a mixture B;
s4, uniformly stirring and mixing the mixture A of S2 and the mixture B of S3 to prepare the feed additive for preventing the intestinal barrier damage of the piglets.
In practical application, the feed additive is added according to 1% of the total mass of the daily feed on the basis of the daily feed formula for the piglets. The specific test is as follows:
180 piglets of 28 days old are selected, and are randomly distributed to 3 treatment groups, namely a control group, an LPS group and a test group according to the weight and the sex by adopting single-factor test design.
Wherein the feed additive of example 1 is not added to the daily feed of the control group and the LPS group; the test group added 1% of the feed additive of example 1 to the daily feed. Each group was repeated 6 times, each time with 10 pigs, for a 28-day test period. On day 28, LPS was intraperitoneally injected into both LPS group and test group, and observation was continued for 5 days after injection. Diarrhea and mortality rates were recorded for each group of piglets and feed-meat ratios were calculated, see table 1.
TABLE 1 Effect of the feed supplement of example 1 on piglet diarrhea rate, mortality and feed-meat ratio
As can be seen from the test results of table 1, the feed additive of example 1 can significantly reduce the mortality, diarrhea rate and feed-meat ratio.
Example 2
A feed additive for preventing intestinal barrier injury of piglets comprises 510 parts by weight of active components and 200 parts by weight of carrier matrix.
The active component is prepared from the following raw materials in parts by weight: 150 parts of taurine, 70 parts of arginine, 70 parts of glutamine, 60 parts of sodium butyrate, 40 parts of zinc oxide, 40 parts of vitamin A, 40 parts of vitamin C and 40 parts of folic acid.
The carrier matrix is prepared from 40% of rice bran, 30% of corncob powder and 30% of bentonite by mass percentage.
The preparation method comprises the following steps:
s1, weighing the raw materials according to the weight part ratio of the feed additive;
s2, adding vitamin A, vitamin C and folic acid into 100 parts of carrier matrix, and stirring and mixing uniformly to obtain a mixture A;
s3, adding taurine, arginine, glutamine, sodium butyrate and zinc oxide into the residual carrier substrate, and uniformly stirring and mixing to obtain a mixture B;
s4, uniformly stirring and mixing the mixture A of S2 and the mixture B of S3 to prepare the feed additive for preventing the intestinal barrier damage of the piglets.
In practical application, the feed additive is added according to 2% of the total mass of the daily feed on the basis of the daily feed formula for the piglets. The specific test is as follows:
240 piglets of 28 days old are selected, and are randomly distributed to 3 treatment groups, namely a control group, an LPS group and a test group according to the weight and the sex by adopting single-factor test design.
Wherein the feed additive of example 2 is not added to the daily feed of the control group and the LPS group; the test group was supplemented with 2% of the feed additive of example 2 in a daily feed. Each group was repeated 8 times, each time with 10 pigs, for a 28-day test period. On day 28, LPS was intraperitoneally injected into both LPS group and test group, and observation was continued for 5 days after injection. Diarrhea and mortality rates were recorded for each group of piglets and feed-meat ratios were calculated, see table 2.
Table 2 effect of feed additive of example 2 on diarrhea rate, mortality rate and feed-to-meat ratio of piglets
As can be seen from the test results of table 2, the feed additive of example 2 can significantly reduce the mortality, diarrhea rate and feed-meat ratio.
Example 3
A feed additive for preventing intestinal barrier injury of piglets comprises 450 parts of active components and 150 parts of carrier matrix according to parts by weight.
The active component is prepared from the following raw materials in parts by weight: 150 parts of taurine, 60 parts of arginine, 60 parts of glutamine, 60 parts of sodium butyrate, 30 parts of zinc oxide, 30 parts of vitamin A, 30 parts of vitamin C and 30 parts of folic acid.
The carrier matrix is prepared from 50% of corncob powder and 50% of corn protein powder by mass percentage.
The preparation method comprises the following steps:
s1, weighing the raw materials according to the weight part ratio of the feed additive;
s2, adding vitamin A, vitamin C and folic acid into 80 parts of carrier matrix, and stirring and mixing uniformly to obtain a mixture A;
s3, adding taurine, arginine, glutamine, sodium butyrate and zinc oxide into the residual carrier substrate, and uniformly stirring and mixing to obtain a mixture B;
s4, uniformly stirring and mixing the mixture A of S2 and the mixture B of S3 to prepare the feed additive for preventing the intestinal barrier damage of the piglets.
In practical application, the feed additive is added according to 1.5 percent of the total mass of the daily feed on the basis of the formula of the daily feed for piglets. The specific test is as follows:
180 piglets of 28 days old are selected, and are randomly distributed to 3 treatment groups, namely a control group, an LPS group and a test group according to the weight and the sex by adopting single-factor test design.
Wherein the feed additive of example 3 was not added to the daily feed of the control group and the LPS group; the test group added 1.5% of the feed additive of example 3 to the daily feed. Each group was repeated 6 times, each time with 10 pigs, for a 28-day test period. On the 28 th day, LPS is injected into the abdominal cavity of both the LPS group and the test group, sampling is carried out 6h after injection, blood is collected through an anterior vena cava, duodenum, jejunum and ileum sections are collected, paraformaldehyde is fixed, and paraffin sections are prepared; scraping mucosa of duodenum and jejunum ileum, quickly freezing with liquid nitrogen, and freezing at-80 deg.C; collecting colon intestine segments, fixing glutaraldehyde, and making electron microscope slices.
TABLE 3 Effect of the feed supplement of example 3 on DAO and D-LA in piglet serum
As can be seen from Table 3, LPS increases intestinal mucosal permeability of piglets, and increases levels of DAO and D-LA in serum, while the feed additive of example 3, which is added in a preventive manner at 1.5%, can significantly reduce levels of DAO and D-LA in serum, which indicates that the feed additive of example 3 can prevent the influence of LPS treatment on intestinal mucosal permeability and protect intestinal mucosal epithelial cells.
TABLE 4 Effect of the feed supplement of example 3 on the duodenum, jejunum, ileum villus length, crypt depth, villus length/crypt depth ratio
FIG. 1 is a comparative scanning electron microscope image showing the effect of the feed supplement of example 3 on villus length, crypt depth, ratio of villus length/crypt depth in duodenum, jejunum, ileum.
FIG. 2 is a comparative scanning electron microscope image showing the effect of the feed supplement of example 3 on the ultrastructure of colonic intestinal mucosal epithelial cells.
In fig. 1 and 2, C represents a control group; LPS means LPS treated group; LPST represents the test group.
As can be seen from Table 4 and FIG. 1, the feed supplement of example 3 was able to increase the length of the villi, decrease crypt depth, and increase the ratio of villi to crypt.
As can be seen from FIG. 2, the feed additive of example 3 was added prophylactically to protect the intestinal mucosal epithelial cells against LPS damage.
TABLE 5 Effect of the feed additive of example 3 on the expression of the genes of duodenum, jejunum, ileum ZO-1, Claudin-1, Oudin
As can be seen from Table 5, the LPS can obviously reduce the gene expression levels of duodenum, jejunum, ileum ZO-1, Claudin-1 and Ocudin of the piglets, and the prophylactic addition of the feed additive in the example 3 can obviously improve the gene expression levels of duodenum, jejunum, ileum ZO-1, Claudin-1 and Ocudin 1 of the piglets, which indicates that the feed additive in the example 3 can protect the tight junction structure of epithelial cells of intestinal mucosa. TABLE 6 influence of feed additives of example 3 on expression of proinflammatory and anti-inflammatory factor releasing genes of epithelial cells of the intestinal mucosa
As shown in Table 6, LPS can increase the expression level of proinflammatory factor genes (TNF-alpha, IL-6, IL-1 beta and IFN-beta) of piglet jejunal mucosal epithelial cells and reduce the levels of anti-inflammatory factors (IL-4 and IL-10). The feed additive of the embodiment 3 is added in a preventive manner, so that the expression level of proinflammatory factor genes (TNF-alpha, IL-6, IL-1 beta and IFN-beta) of jejunal mucosal epithelial cells of piglets can be obviously reduced, and the levels of anti-inflammatory factors (IL-4 and IL-10) are improved.
Example 4
A feed additive for preventing intestinal barrier injury of piglets is divided into two groups of X group and Y group, wherein the X group feed additive consists of 640 parts of X group active components and 350 parts of carrier matrix according to parts by weight; the Y group feed additive consists of 350 parts of Y group active components and 150 parts of carrier matrix.
The X group of active components are prepared from the following raw materials in parts by weight: 200 parts of taurine, 80 parts of arginine, 80 parts of glutamine, 80 parts of sodium butyrate, 50 parts of zinc oxide, 50 parts of vitamin A, 50 parts of vitamin C and 50 parts of folic acid.
The Y group active component is prepared from the following raw materials in parts by weight: 200 parts of taurine, 50 parts of vitamin A, 50 parts of vitamin C and 50 parts of folic acid.
The carrier matrix is prepared from 50% of corncob powder and 50% of corn protein powder by mass percentage.
The specific preparation method of the group X feed additive comprises the following steps:
s101, weighing raw materials according to the weight part ratio of the feed additive to prepare X groups of feed additives;
s102, adding vitamin A, vitamin C and folic acid into 100 parts of carrier matrix, and uniformly stirring and mixing to obtain a mixture A;
s103, taking the rest carrier matrix, adding taurine, arginine, glutamine, sodium butyrate and zinc oxide, and uniformly stirring and mixing to obtain a mixture B;
and S104, uniformly stirring and mixing the mixture A of the S102 and the mixture B of the S103 to prepare the group X feed additive for preventing the intestinal barrier injury of the piglets.
The specific preparation method of the Y group feed additive comprises the following steps:
s201, weighing raw materials according to the weight part ratio of the feed additive to prepare a Y group of feed additive;
s202, adding vitamin A, vitamin C and folic acid into 100 parts of carrier matrix, and uniformly stirring and mixing to obtain a mixture A;
s203, adding taurine into the residual carrier matrix, and uniformly stirring and mixing to obtain a mixture B;
and S204, uniformly stirring and mixing the mixture A of the S202 and the mixture B of the S203 to prepare the group Y feed additive for preventing the intestinal barrier injury of the piglets.
In practical application, the X group feed additive and the Y group feed additive are added on the basis of a piglet daily feed formula according to 1% of the total mass of daily feeds. The specific test is as follows:
240 piglets of 28 days old are selected, and are randomly distributed to 4 treatment groups, namely a control group, an LPS group, an experiment X group and an experiment Y group according to the weight and the sex by adopting single-factor experiment design.
Wherein the feed additive of example 4 was not added to the daily feed of the control group and the LPS group; test group X1% of feed additives of group X were added to the daily feed; test group Y1% of the feed additives of group Y were added to the daily feed. Each group was repeated 6 times, each time with 10 pigs, for a 28-day test period. On day 28, LPS groups, test X and test Y groups were each intraperitoneally injected with LPS, and the observation was continued for 5 days after the injection. Recording the diarrhea rate and mortality rate of each group of piglets, and calculating the feed-meat ratio, see table 7; blood was collected and tested for DAO and D-LA.
Table 7 effect of group X and group Y feed additives of example 4 on piglet diarrhea rate, mortality and feed-to-meat ratio
As can be seen from the test results of table 7, the feed additive of example 4 can significantly reduce the mortality, diarrhea rate and feed conversion ratio, and the test X group is superior to the test Y group.
TABLE 8 Effect of the feed supplement of example 4 on DAO and D-LA in piglet serum
As can be seen from the test results of Table 8, the feed additive of example 4 was able to significantly reduce DAO and D-LA, and the test X group was superior to the test Y group.
The present invention is not limited to the above preferred embodiments, and any modifications, equivalent substitutions and improvements made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (8)
1. A feed additive for preventing intestinal barrier injury of piglets is characterized by comprising active components and a carrier matrix; the active component is prepared from the following raw materials in parts by weight:
200 parts of taurine 150-.
2. The feed additive for preventing intestinal barrier injury of piglets according to claim 1, wherein the carrier matrix is any one of or a mixture of at least two of bran meal, rice bran, unite bran, corncob meal, corn gluten meal, zeolite powder and bentonite.
3. The feed additive for preventing intestinal barrier injury of piglets according to claim 1, wherein the mass ratio of the active component to the carrier matrix is 350-640: 150-350.
4. The feed additive for preventing piglet intestinal barrier injury according to claim 1, wherein the mass ratio of vitamin A to vitamin C to folic acid is 1: 1: 1.
5. a preparation method of a feed additive for preventing intestinal barrier injury of piglets is characterized by comprising the following steps:
s1, weighing the raw materials according to the weight part ratio of the feed additive of any one of claims 1 to 4;
s2, uniformly mixing a part of carrier matrix with vitamin A, vitamin C and folic acid to obtain a mixture A;
s3, uniformly mixing the rest carrier matrix with taurine, arginine, glutamine, sodium butyrate and zinc oxide to obtain a mixture B;
s4, uniformly mixing the mixture A of the S2 and the mixture B of the S3 to prepare the feed additive for preventing the intestinal barrier damage of the piglets.
6. The method for preparing a feed additive for preventing intestinal barrier injury of piglets as claimed in claim 5, wherein the total amount of the active components is 350-640 parts by weight; the total amount of the carrier matrix is 150-350 parts by weight.
7. The application of the feed additive for preventing the barrier injury of the intestinal tracts of the piglets according to any one of claims 1 to 4, wherein the feed additive for preventing the barrier injury of the intestinal tracts of the piglets is used for being added into the daily ration of the piglets to prepare the piglet feed.
8. The use of the feed additive for preventing the barrier injury of the intestinal tract of the piglets according to the claim 7, wherein the dosage of the feed additive for preventing the barrier injury of the intestinal tract of the piglets accounts for 1-2% of the total mass of the feed for the piglets.
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