CN114009423A

CN114009423A - Mouse specimen storage method for tumor drug development

Info

Publication number: CN114009423A
Application number: CN202111216520.2A
Authority: CN
Inventors: 孙屏; 吕慧; 李平; 程洁
Original assignee: Wuxi No 2 Peoples Hospital
Current assignee: Wuxi No 2 Peoples Hospital
Priority date: 2021-08-31
Filing date: 2021-10-19
Publication date: 2022-02-08
Anticipated expiration: 2041-10-19
Also published as: CN114009423B

Abstract

The embodiment of the invention provides a method for storing mouse specimens for developing tumor drugs, which comprises S1, wherein according to the number of visceral organs to be stored, the end covers of corresponding accommodating cavities are taken down from a plurality of accommodating bodies, the adjusting blocks are taken out, the adjusting blocks are lowered to the bottom by the rest of the unnecessary accommodating bodies to block the side holes, then the adjusting rods are locked on the end covers through locking devices, and the end covers are fixed on the accommodating bodies; s2, injecting formaldehyde solution into the exposed accommodating bodies, synchronously injecting the formaldehyde solution into a plurality of accommodating bodies due to the existence of the connecting pipes, and injecting the formaldehyde solution into a conventional liquid level when the liquid level of each accommodating body is equal to the height of the other accommodating body; and S3, placing the viscera in the accommodating body with the exposed accommodating cavity, and continuously filling the formaldehyde solution until the liquid level is flush with the accommodating body.

Description

Mouse specimen storage method for tumor drug development

Technical Field

The invention relates to the technical field of tumor drug development, in particular to a mouse specimen storage method for tumor drug development.

Background

Anti-malignant tumor cell invasion and metastasis medicine; chemoradiotherapy sensitizing drugs; chemoprevention of tumors and for the treatment of precancerous lesions; new drugs acting on tumor cell signaling systems; other novel antineoplastic agents; drugs for tumor adjuvant therapy (including analgesia, antiemetic, immune function enhancement, hypercalcemia caused by tumors, and the like) and the like are researched by taking experimental animals as models, wherein the experimental animals are mainly mice. After the experiment is finished, the mouse is dissected, the experimenter stores the needed organs or routine organs (such as heart, liver, spleen, lung, kidney and the like) in the formaldehyde solution for long-term preservation, and the organs can be taken out for pathological examination when necessary in the future.

But put into repeatedly many times and draw the mouse sample of soaking into formaldehyde solution, complex operation on the one hand, on the other hand formaldehyde has human injury nature, has the injury to the experimenter health.

Whether a plurality of mouse specimens after tumor experiments can be placed in one step or not is determined, so that the operation steps and time are saved, and the technical problem to be solved is solved.

Disclosure of Invention

The embodiment of the application provides a method for storing a mouse specimen for tumor drug development, which solves the technical problem of the mouse specimen after a plurality of tumor experiments are put in one time.

The embodiment of the application provides an operation method of a mouse specimen storage method for tumor drug development,

the memory includes:

a base plate;

the accommodating body is arranged on the bottom plate and is provided with an accommodating cavity capable of accommodating visceral organs; a side hole is formed in the inner wall of the accommodating cavity, the side hole is a through hole, and the side hole is communicated with the accommodating cavity and the outside; the number of the accommodating bodies is at least two, and the two accommodating bodies are arranged at intervals;

the end cover is detachably connected to the accommodating body, and the accommodating cavity is sealed through the end cover;

the connecting pipe is hollow and is connected and communicated with the side holes on the two accommodating bodies, and the accommodating cavities of the two accommodating bodies are communicated through the connecting pipe; and

an adjustment assembly, the adjustment assembly comprising:

the adjusting device comprises an adjusting rod, an adjusting block and a locking device; the adjusting rod is connected with the adjusting block and the locking device respectively, and the adjusting rod can be locked on the end cover based on the locking device so as to be fixedly connected with the end cover.

The adjusting rod comprises a first rod body and a second rod body, the first rod body and the second rod body are an upper part and a lower part of a whole rod body, the first rod body penetrates through the end cover, one part of the first rod body is positioned in the end cover, and the other part of the first rod body is positioned outside the end cover; the second rod body is positioned below the first rod body, connected with the first rod body and positioned in the accommodating cavity; it is characterized in that the preparation method is characterized in that,

the use method of the memory comprises the following steps:

s1, according to the number of visceral organs to be stored, the end covers of the corresponding accommodating cavities are taken down from the accommodating bodies, the adjusting blocks are all taken out, the adjusting blocks are lowered to the bottom by the rest of the unnecessary accommodating bodies to block the side holes, then the adjusting rods are locked on the end covers through the locking devices, and the end covers are fixed on the accommodating bodies;

s2, injecting formaldehyde solution into the exposed accommodating bodies, synchronously injecting the formaldehyde solution into a plurality of accommodating bodies due to the existence of the connecting pipes, and injecting the formaldehyde solution into a conventional liquid level when the liquid level of each accommodating body is equal to the height of the other accommodating body;

s3, placing the viscera in the accommodating body with the exposed accommodating cavity, and then continuously filling the formaldehyde solution until the liquid level is flush with the accommodating body;

s4, flatly placing the adjusting blocks in the containing cavities at the top of the liquid level, simultaneously releasing a locking device of an idle containing cavity, lifting the adjusting blocks of the idle containing cavity upwards, and at the moment, the liquid level of the formaldehyde solution in the containing cavities in which the adjusting blocks are placed begins to descend, and the adjusting blocks also descend along with the liquid level;

s5, at the moment, the height of the adjusting block in each accommodating cavity can be freely adjusted according to the size of the visceral organ put in each accommodating cavity, so that the volume of the first accommodating cavity is matched with the volume of the visceral organ;

s6, assembling the containing body and the end cover corresponding to the containing body into a whole, and locking the adjusting rod on the end cover through a locking device;

and S7, when the viscera needs to be taken out, all the adjusting rods are unlocked, the adjusting blocks of the accommodating cavities can be arbitrarily lifted according to the principle of the communicating vessels until the adjusting blocks are taken out, and the viscera in the accommodating cavities are exposed at the moment.

Further, S5 is replaced by continuously lifting the height of the adjusting block of the idle accommodating cavity, so that the height of the adjusting block in each accommodating cavity is adjusted through the principle of the communicating vessels.

Further, S6 is replaced by that the adjusting rod of the idle accommodating cavity is locked on the end cover through a locking device.

Further, S6 is replaced by a special accommodating cavity which is a free accommodating cavity, a switch is arranged on a connecting pipe on the free accommodating cavity, so that after other accommodating cavities are adjusted, the switch on the connecting pipe is closed, the free accommodating cavity is isolated from the communicating vessel, and the adjusting rod of the free accommodating cavity at the moment does not need to be locked.

One or more technical solutions in the embodiments of the present application have at least one or more of the following technical effects:

1. in the embodiment of the application, between the first holding chamber of a plurality of that switches on each other, can be through a regulating block of lift because the principle of linker to the height of other regulating blocks of control, thereby reach the purpose of adjusting other holding chamber volumes simultaneously, thereby reach and put into mouse sample simultaneously, adjust the technical effect of antiseptic quality in the container simultaneously. Resulting in the ultimate goal of saving operating steps and time.

2, as another scheme in this application embodiment, can set up the holding chamber specially and be idle holding chamber, the connecting pipe on the idle holding chamber is provided with the switch, like this when other holding chambeies finish adjusting, can close the switch on the connecting pipe, separates idle holding chamber from the linker, and the regulation pole in idle holding chamber this moment just need not lock. Therefore, the operation is simpler on the basis of not changing the technical effect.

Drawings

FIG. 1 is a schematic axial view of a memory according to an embodiment of the present disclosure;

FIG. 2 is a schematic top view of a memory according to an embodiment of the present disclosure;

FIG. 3 is a schematic cross-sectional view of a memory according to an embodiment of the present application;

FIG. 4 is a schematic sectional view showing the structure of a storage method of a mouse specimen for tumor drug development in the embodiment of the present application when storing organs;

FIG. 5 is a schematic sectional view showing the organ storage according to the embodiment of the present application;

FIG. 6 is a schematic cross-sectional view of a memory according to an embodiment of the present application;

FIG. 7 is a schematic cross-sectional view of a memory according to an embodiment of the present application;

reference numerals: 1. a base plate; 2. an accommodating body; 21. an accommodating cavity; 211. a first accommodating cavity; 212. a second accommodating cavity; 22. a side hole; 3. an end cap; 31. an end cap seal; 4. an adjustment assembly; 41. adjusting a rod; 411. a first rod body; 412. a second rod body; 42. an adjusting block; 421. a balance hole; 422. a sealing strip; 43. a locking device; 5. a connecting pipe; 6. filtering with a screen; 7. a cover plate; 71. a cover plate side; 8. organs.

Detailed Description

In order to better understand the technical solution, the technical solution will be described in detail with reference to the drawings and the specific embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the present application and are not intended to limit the present application.

The method for storing mouse specimen for developing tumor medicine, referring to the attached fig. 1 and 2 of the specification, comprises a storage for storing animal viscera, which comprises the following steps: the device comprises a bottom plate 1, an accommodating body 2, an end cover 3, a connecting pipe 5, a filter screen 6, an adjusting component 4 and a cover plate 7; wherein, the holding body 2 is provided with a plurality ofly and is located bottom plate 1, based on connecting pipe 5 intercommunication between a plurality of holding bodies 2 to based on the connected state between a plurality of holding bodies 2 of adjusting part 4 control, reach the purpose that this device can once inject formaldehyde solution for a plurality of holding bodies 2. Of course, the device is used not only for mice but also for other animal specimens, such as rats, rabbits, monkeys, etc.

The bottom plate 1, refer to the attached figure 2 in the specification, and the bottom plate 1 is a plate body and serves as a carrier of the accommodating body 2. It can be understood that the attached figure 2 of the specification is a plane style diagram for placing the mouse viscera, the accommodating bodies 2 are arranged on the bottom plate 1 according to the arrangement mode of the viscera 8 in the mouse body, and the storage positions of the viscera 8 are selected based on the actual physiological structure of the mouse, so that under the condition that the labels are fuzzy or the visual angle is poor, the types of the viscera 8 stored in the accommodating bodies 2 can be quickly determined.

The container 2, referred to in the description and attached to fig. 3, is used for containing the mouse's viscera 8. The accommodating body 2 is arranged on the bottom plate 1, and the accommodating body 2 is provided with an accommodating cavity 21 capable of accommodating the visceral organ 8; a side hole 22 (fig. 6) is arranged on the inner wall of the accommodating cavity 21, the side hole 22 is a through hole, and the side hole 22 is communicated with the accommodating cavity 21 and the outside; at least two accommodating bodies 2 are arranged, and the two accommodating bodies 2 are arranged at intervals; if the two containers 2 are two containers with different cross-sectional areas, after the formaldehyde solution is filled, the formaldehyde solution stored between the two containers 2 is different according to the liquid level of the two containers 2 with different cross-sectional areas, and then the containers 2 are selected and placed according to the volume of the viscera to be stored. It should be noted that the accommodating body 2 and the bottom plate 1 may be an integral body, and the accommodating body 2 and the bottom plate 1 may also be a separate structure fixedly connected. It can be understood that the receiving cavity 21 in the receiving body 2 is opened upward, and the end cap 3 is connected to the receiving body 2 from top to bottom.

The size of each of the receiving bodies 2 is designed according to the size of a normal organ in scientific research, and a label (indicating what organ is) is provided beside each of the receiving bodies. In addition, it is necessary to reserve a plurality of containers 2 for holding a plurality of special organs for the experimenter to store a plurality of special organs, tumor tissues, special pathological tissues (abbreviated as unconventional organs) discovered unexpectedly in the dissection, and the like. Of course, if the containing cavities of the conventional visceral organs are not used up, the non-conventional visceral organs can be used by selecting one containing cavity of the conventional visceral organs which is not used yet and has almost the same size, and only the label needs to be changed.

The end cap 3, refer to fig. 3 of the specification, is used for cooperating with the accommodating body 2 to construct an accommodating cavity 21. The end cap 3 is detachably connected to the accommodating body 2, such as by sliding connection.

The connecting pipe 5, referring to the attached fig. 1 and 3, is used for connecting the accommodating cavities 21 of two or more accommodating bodies 2. The connecting pipe 5 is hollow, the connecting pipe 5 is connected with and communicated with the side holes 22 on the two accommodating bodies 2, and the accommodating cavities 21 of the two accommodating bodies 2 are communicated through the connecting pipe 5. It will be understood that the connecting tube 5 may be integral with the housing 2 or may be later ultrasonically welded together. Of course, in order to facilitate the observation of the usage of the device, the accommodating body 2, the end cap 3 and the connecting pipe 5 are preferably made of transparent materials.

It should be noted that the connection tube 5 may also be applied to the bottom of the two containers 2, so that when the containers 2 are connected and combined with each other, the liquid may flow between all the containers 2, thereby preventing the formaldehyde solution from flowing into the desired container 2 due to the connection circuit between the containers 2 being closed.

The filter screen 6, the filter screen 6 is fixed to be set up in the side opening 22 or the connecting pipe 5 of holding chamber 21, avoids the viscera 8 card in side opening 22 through filter screen 6, avoids the viscera 8 to get into in the connecting pipe 5.

The regulating assembly 4, referred to in the description in the attached figures 3-4, is used, on the one hand, to seal the lateral holes 22 of the containing body 2 and, on the other hand, to control the amount of formaldehyde solution inside the containing body 2. The adjustment assembly 4 comprises: an adjustment lever 41, an adjustment block 42, and a locking device 43; the adjusting rod 41 is connected with the adjusting block 42 and the locking device 43 respectively, and the adjusting rod 41 can be locked on the end cover 3 based on the locking device 43, so as to be fixedly connected with the end cover 3.

The adjusting rod 41, the adjusting rod 41 includes a first rod 411 and a second rod 412, and preferably, the first rod 411 and the second rod 412 are upper and lower portions of a whole rod. The first rod 411 penetrates the end cap 3, and a part of the first rod 411 is located inside the end cap 3, and the other part is located outside the end cap 3. In one embodiment, the first rod 411 is slidably connected to the end cap 3, and a locking device 43 is further connected to an outer portion of the first rod 411 opposite to the end cap 3. The second rod 412 is located below the first rod 411, the second rod 412 is connected to the first rod 411, and the second rod 412 is located in the accommodating cavity 21.

Referring to fig. 5, when the internal organs 8 are too large or one accommodating body 2 stores a plurality of internal organs 8, the lower adjusting block 42 can be detached to increase the actual available space in the accommodating cavity 21. Or the adjusting block 42 is designed to have an upper end surface recessed inwards, and when the available space of the accommodating cavity 21 is insufficient when the adjusting block 42 is used in the forward direction, the adjusting block 42 can be removed and connected in a mode of being connected to the adjusting rod 41 in the reverse direction, so that the available space of the accommodating cavity 21 is increased.

The adjusting block 42 is positioned in the accommodating cavity 21, and the adjusting block 42 is connected with the second rod body 412; and the adjusting block 42 corresponds to and is attached to the side hole 22 on the inner wall of the accommodating cavity 21. Based on the position of the first rod 411 relative to the end cover 3 is adjustable, the first rod 411 acts on the adjusting block 42 in a linkage manner, and the adjusting block 42 can enable the side hole 22 on the accommodating body 2 to be opened or closed. It will be appreciated that the end cap 3 may be removed separately or the adjustment assembly 4 may be raised and lowered separately.

It should be noted that, in order to improve the function of the adjusting assembly 4 in the present device, the accommodating cavity 21 is transversely sealed by the side surface of the adjusting block 42, the accommodating cavity 21 is divided into a first accommodating cavity 211 and a second accommodating cavity 212 by the adjusting block 42, and the volume of the first accommodating cavity 211 is adjustable by the adjusting block 42. It can be noted that the sidelines of the adjusting block 42 relative to the two side surfaces of the first accommodating cavity 211 and the second accommodating cavity 212 are both designed to be chamfered, so that the adjusting block 42 can move up and down along the inner wall of the accommodating cavity 21. Referring to fig. 3-4 of the specification, a balance hole 421 is formed in the adjusting block 42, the balance hole 421 is a through hole, and two ends of the balance hole 421 are respectively communicated with the first accommodating cavity 211 and the second accommodating cavity 212.

Based on the constructed adjusting component 4, the volume of the first accommodating cavity 211 in the accommodating body 2 can be adjusted at will when the accommodating body accommodates animal viscera, so as to accommodate animal viscera with different shapes and volumes. The liquid level of the formaldehyde does not need to be too high in small organs such as gall bladder, adrenal gland and the like or flat organs such as spleen, thymus and the like. The heart, the liver and the like are three-dimensional structures, and the liquid level of the required accommodating cavity is high.

More importantly, one adjusting block 42 can be lifted and lowered among a plurality of first accommodating cavities 211 communicated with each other, and the heights of other adjusting blocks 42 can be controlled due to the principle of the communicating device.

Further, referring to the attached drawing 6 of the specification, the sealing strip 422 is arranged on the side face of the adjusting block 42 in a surrounding mode, the sealing strip 422 is attached to the inner wall of the accommodating cavity 21, the material of the sealing strip 422 is selected, if the adjusting block 42 is made of metal, the sealing strip 422 is made of rubber, so that the adjusting block 42 has enough hardness to be connected with the adjusting rod 41 in a threaded mode under the condition that the adjusting block 42 can be sealed with the accommodating cavity 21. It will be appreciated that the sealing strip 422 may be provided in multiple layers in an up-down relationship on the adjustment block 42 to enhance the sealing effect; and when the two side sealing strips 422 are respectively positioned at the upper side and the lower side of the side hole 22, the amount of formaldehyde solution waste is reduced.

The cover plate 7, refer to fig. 7 of the specification, is used for making the appearance of the device smooth, and is convenient for stacking and storing. The cover plate 7 is connected with the bottom plate 1 in a bonding and clamping manner or a clamping manner by utilizing a clamp. And forms a containing cavity 21 for covering the containing body 2 and the end cover 3. The cover plate 7 comprises a cover plate side face 71, and the cover plate side face 71 is clamped and fixed with the end cover 3 or the accommodating body 2. It should be noted that, in the case of the cover plate 7, the accommodating body 2 and/or the end cap 3 are preferably spaced apart from the edge of the base plate 1, so as to facilitate the attachment and connection of the edges of the cover plate 7 and the base plate 1.

A method for storing a mouse specimen for tumor drug development comprises the following steps:

and S1, taking down the end covers 3 of the corresponding accommodating cavities from the accommodating bodies 2 according to the number of the viscera 8 required to be stored, and taking out all the adjusting blocks 42. If six organs 8 need to be stored, the end covers 3 on the corresponding six accommodating bodies 2 in the device are opened, so that the accommodating cavities of the six accommodating bodies 2 are exposed. The remaining unwanted container 2 lowers the regulating block 42 to the bottom to block the side hole 22. Then, the adjusting rod 41 is locked on the end cover 3 through the locking device 43, and the end cover 3 is fixed on the accommodating body 2, so that the side hole 22 is blocked, and the formaldehyde solution cannot enter the accommodating cavity of the unused accommodating body 2 through the connecting pipe 5.

And S2, filling the formaldehyde solution into the exposed accommodating bodies 2, and filling the formaldehyde solution into a plurality of accommodating bodies 2 synchronously due to the existence of the connecting pipes 5, wherein the liquid level of each accommodating body 2 is equal to the height. It should be noted that, a scale mark may be provided on the accommodating body 2, so as to fill the formaldehyde solution to a conventional liquid level based on the scale mark. The conventional level may be known based on past filling experience.

And S3, after the formaldehyde solution is filled in the accommodating body 2, placing the viscera 8 in the accommodating body 2 with the exposed accommodating cavity, and then continuously filling the formaldehyde solution until the liquid level is flush with the accommodating body 2.

S31, flatly placing the adjusting blocks 42 in the containing cavities at the top of the liquid level, releasing the locking device 43 of an idle containing cavity, lifting the adjusting blocks 42 of the idle containing cavity upwards, enabling the liquid level of the formaldehyde solution in the containing cavity where the adjusting blocks 42 are placed to start to descend, and enabling the adjusting blocks 42 to descend along with the liquid level.

S4, at this time, the height of the adjusting block 42 in each accommodating cavity can be freely adjusted according to the volume of the visceral organ placed in each accommodating cavity, so that the volume of the first accommodating cavity 211 matches the volume of the visceral organ, that is, based on the fact that the adjusting block 42 is immersed in the formaldehyde of the accommodating body 2, the part of the formaldehyde encroached by the adjusting block 42 is squeezed to flow into the other accommodating bodies 2 which are not covered, so that the liquid level of the formaldehyde in the other accommodating bodies 2 is raised, and because of the principle of the communicating vessel, the height of the adjusting block 42 in the other accommodating bodies 2 is raised, and vice versa. The height of the adjusting block 42 of the vacant accommodating cavity can be continuously lifted, so that the height of the adjusting block 42 in each accommodating cavity can be adjusted through the principle of the communicating vessel. That is, the volume of the other first receiving cavities 211 can be uniformly adjusted by adjusting the height of the adjusting block 42 of the empty receiving cavity, or the volume of each first receiving cavity 211 can be adjusted by adjusting the height of the adjusting block 42 in each receiving cavity individually.

S5, the accommodating body 2 and the corresponding end cap 3 of the accommodating body 2 are assembled into a whole, and the adjusting lever 41 is locked to the end cap 3 by the locking device 43. It is of course also possible to unlock or to directly remove the end cap 3. But the adjustment rod 41 of the free accommodation chamber must be locked to the end cap 3 by means of the locking device 43.

S6, the containing cavity can be specially set to be an idle containing cavity, the connecting pipe 5 on the idle containing cavity is provided with a switch, therefore, after other containing cavities are adjusted, the switch on the connecting pipe 5 can be closed, the idle containing cavity is isolated from the communicating vessel, and the adjusting rod 41 of the idle containing cavity at the moment does not need to be locked.

And S7, covering the cover plate on the bottom plate.

And S8, when the viscera needs to be taken out, all the adjusting rods 41 are unlocked, the adjusting blocks 42 of the accommodating cavities can be arbitrarily lifted according to the principle of the communicating vessels until the adjusting blocks 42 are taken out, and the viscera in the accommodating cavities are exposed at the moment.

Claims

1. The operation method of the mouse specimen storage method for tumor drug development comprises a storage device, wherein the storage device comprises: a base plate;

an adjustment assembly, the adjustment assembly comprising:

the use method of the memory comprises the following steps:

2. The operating method as claimed in claim 1, wherein S5 is replaced by continuously raising the height of the adjustment block of the vacant accommodation chamber, thereby adjusting the height of the adjustment block in each accommodation chamber by the principle of a communicating vessel.

3. The method of claim 1, wherein S6 is replaced by locking the adjustment rod of the empty receiving chamber to the end cap by a locking device.

4. The operating method according to claim 1, wherein S6 is replaced by specially providing a free accommodating chamber, and the connecting tube on the free accommodating chamber is provided with a switch, so that when the other accommodating chambers are adjusted, the switch on the connecting tube is closed to separate the free accommodating chamber from the communicating vessel, and the adjusting rod of the free accommodating chamber does not need to be locked.