CN114002047A - Tobacco product aerosol condensation, trapping and extraction device and trapping and extraction method - Google Patents
Tobacco product aerosol condensation, trapping and extraction device and trapping and extraction method Download PDFInfo
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- CN114002047A CN114002047A CN202111285468.6A CN202111285468A CN114002047A CN 114002047 A CN114002047 A CN 114002047A CN 202111285468 A CN202111285468 A CN 202111285468A CN 114002047 A CN114002047 A CN 114002047A
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- 239000000443 aerosol Substances 0.000 title claims abstract description 90
- 238000000605 extraction Methods 0.000 title claims abstract description 54
- 238000009833 condensation Methods 0.000 title claims abstract description 41
- 230000005494 condensation Effects 0.000 title claims abstract description 41
- 235000019505 tobacco product Nutrition 0.000 title claims abstract description 36
- 238000001926 trapping method Methods 0.000 title claims abstract description 9
- 238000010828 elution Methods 0.000 claims abstract description 48
- 235000019504 cigarettes Nutrition 0.000 claims abstract description 47
- 238000006073 displacement reaction Methods 0.000 claims abstract description 44
- 238000001816 cooling Methods 0.000 claims abstract description 41
- 230000000391 smoking effect Effects 0.000 claims description 52
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 32
- 238000000034 method Methods 0.000 claims description 24
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 claims description 15
- 239000012156 elution solvent Substances 0.000 claims description 15
- 239000007788 liquid Substances 0.000 claims description 14
- 239000000203 mixture Substances 0.000 claims description 11
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims description 8
- 235000011089 carbon dioxide Nutrition 0.000 claims description 8
- UGFAIRIUMAVXCW-UHFFFAOYSA-N Carbon monoxide Chemical compound [O+]#[C-] UGFAIRIUMAVXCW-UHFFFAOYSA-N 0.000 claims description 6
- 239000003546 flue gas Substances 0.000 claims description 6
- 238000002156 mixing Methods 0.000 claims description 5
- 238000009413 insulation Methods 0.000 claims description 3
- 239000000779 smoke Substances 0.000 abstract description 20
- 238000011156 evaluation Methods 0.000 abstract description 9
- 239000002904 solvent Substances 0.000 abstract description 9
- 230000001988 toxicity Effects 0.000 abstract description 5
- 231100000419 toxicity Toxicity 0.000 abstract description 5
- 238000000338 in vitro Methods 0.000 abstract description 4
- 239000002609 medium Substances 0.000 description 16
- SNICXCGAKADSCV-JTQLQIEISA-N (-)-Nicotine Chemical compound CN1CCC[C@H]1C1=CC=CN=C1 SNICXCGAKADSCV-JTQLQIEISA-N 0.000 description 9
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 9
- 229960002715 nicotine Drugs 0.000 description 9
- SNICXCGAKADSCV-UHFFFAOYSA-N nicotine Natural products CN1CCCC1C1=CC=CN=C1 SNICXCGAKADSCV-UHFFFAOYSA-N 0.000 description 9
- 238000010586 diagram Methods 0.000 description 6
- 239000002245 particle Substances 0.000 description 5
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 3
- GGNALUCSASGNCK-UHFFFAOYSA-N carbon dioxide;propan-2-ol Chemical compound O=C=O.CC(C)O GGNALUCSASGNCK-UHFFFAOYSA-N 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 2
- 231100000750 In vitro toxicology Toxicity 0.000 description 2
- 241000208125 Nicotiana Species 0.000 description 2
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 2
- 238000004587 chromatography analysis Methods 0.000 description 2
- 239000003480 eluent Substances 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 239000005457 ice water Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000006143 cell culture medium Substances 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000003571 electronic cigarette Substances 0.000 description 1
- 235000011187 glycerol Nutrition 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 230000002110 toxicologic effect Effects 0.000 description 1
- 231100000027 toxicology Toxicity 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/4055—Concentrating samples by solubility techniques
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D11/00—Solvent extraction
- B01D11/04—Solvent extraction of solutions which are liquid
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/4055—Concentrating samples by solubility techniques
- G01N2001/4061—Solvent extraction
Abstract
The invention provides a tobacco product aerosol condensation, trapping and extraction device and a trapping and extraction method, and relates to the technical field of tobacco products, wherein the device comprises a trapping part, a cooling cavity and an elution part, the trapping part comprises a spiral pipe, a first end pipe and a second end pipe, a condensing medium is arranged in the cooling cavity, and the inner diameter of the cooling cavity is not less than the length of the spiral pipe; elution portion includes first branch pipe, second branch pipe and communicating pipe, is provided with the piston in communicating pipe, and the communicating pipe outside is provided with displacement platform, and displacement platform passes through the connecting rod and is connected with the piston, is provided with on the communicating pipe lateral wall to be used for making things convenient for the gliding spout of connecting rod for drive piston reciprocating motion in the communicating pipe when displacement platform reciprocating displacement motion. The invention has simple structure, can meet the requirements of trapping the smoke of the heated cigarette, can realize extraction by using a solvent as little as possible, and further meets the experimental requirements of the in vitro toxicity evaluation of the aerosol of the novel tobacco products.
Description
Technical Field
The invention relates to the technical field of tobacco products,
in particular, the invention relates to a tobacco product aerosol condensation, trapping and extraction device and a trapping and extraction method.
Background
In recent years, new tobacco products such as heating cigarettes, electronic cigarettes, hookahs and the like are emerging, the safety of the new tobacco products is widely concerned by the society, and effective toxicological means are urgently needed to carry out biological safety evaluation. At present, a great deal of literature reports adopt methods such as in vitro toxicology to carry out biological safety evaluation on the aerosol of the traditional cigarette and the novel tobacco product, but the aerosol needs to be trapped firstly before the evaluation.
The traditional method for trapping the aerosol of the tobacco products at present is to trap the novel aerosol of the whole smoke of the tobacco by using a trapping bottle containing PBS or cell culture medium, and has the great defects that the concentration of the aerosol component in a trapping substance is very low, and the trapping bottle cannot trap a lot of aerosol components well; with the proposal of the method for capturing the aerosol by the cambridge filter disc, the method is adopted to replace the traditional method for capturing the aerosol at present, namely, the Cambridge filter disc is used for shaking extraction by using an organic solvent (DMSO, ethanol or the like) after being trapped, the concentration of the trapped substance can be improved to a certain degree, for example, chinese patent invention CN106769750A discloses a method for testing and calculating the quantity interception efficiency of cambridge filter disc to electronic smoke sol, which adopts a smoking machine to smoke electronic smoke through a trap, collects the electronic smoke sol by the cambridge filter disc in the trap, an aerosol particle number and particle size tester is connected into a suction pipeline between the catcher and the smoking machine through a three-way joint and used for measuring the aerosol particle number and the particle size, and calculating the quantity interception efficiency of the Cambridge filter disc on the electronic smoke sol by measuring the aerosol particle number when the Cambridge filter disc is added into and removed from the catcher. The method is simple to operate, and can quickly test and calculate the quantity interception efficiency of the cambridge filter disc on different electronic smoke sols.
However, the above-mentioned method of trapping aerosols has significant drawbacks: after the aerosol is extracted by the solution, the aerosol is easy to contain the extraction solution when the aerosol is extracted, so that the detection result of the extracted aerosol is influenced.
Therefore, in order to solve the problems, it is necessary to design a reasonably efficient condensation, collection and extraction device for aerosol of tobacco products.
Disclosure of Invention
The invention aims to provide a tobacco product aerosol condensation and collection extraction device which is simple in structure, can collect smoke of a heated cigarette, can realize extraction by using a solvent as little as possible, and further meets the requirements of a novel tobacco product aerosol in-vitro toxicity evaluation experiment.
In order to achieve the purpose, the invention is realized by adopting the following technical scheme:
the utility model provides a tobacco products aerosol condensation entrapment extraction element, including the entrapment portion that is used for the entrapment aerosol that produces when the cigarette suction, be used for during entrapment portion entrapment aerosol for the cooling chamber that the entrapment portion cooled down and be used for extract behind the entrapment portion entrapment aerosol the elution portion of the aerosol in the entrapment portion, the entrapment portion includes the spiral pipe, set up in the first end pipe of spiral pipe one end with set up in the spiral pipe is kept away from the second end pipe of the one end of first end pipe, be provided with the condensing medium in the cooling intracavity, just the internal diameter in cooling chamber is not less than the length of spiral pipe for insert when the first end pipe at spiral pipe both ends and second end pipe are connected to suction end and cigarette end respectively to the flue gas that the cigarette was supported with the cooling intracavity is carried out the condensation and is convenient for suction end entrapment aerosol; elution portion is including being used for behind the entrapment portion entrapment aerosol with first branch pipe of first end pipe intercommunication, be used for behind the entrapment portion entrapment aerosol with the second branch pipe of second end pipe intercommunication and be used for the intercommunication communicating pipe of first branch pipe and second branch pipe, be provided with the piston in communicating pipe, the communicating pipe outside be provided with parallel arrangement's displacement platform communicating pipe, displacement platform pass through the connecting rod with the piston is connected, it is used for conveniently to be provided with on the lateral wall communicating pipe the gliding spout of connecting rod for drive when displacement platform reciprocating displacement motion the piston in reciprocating motion in the communicating pipe.
Preferably, the condensation medium is a mixture of powdered dry ice and isopropanol.
Preferably, the cooling cavity is a heat insulation piece.
Preferably, the volume of the communication pipe is not less than twice the volume of the spiral pipe.
Preferably, a holding rod is connected between the first end tube and the second end tube.
Preferably, the inner diameter of the spiral pipe is 8mm, and the length of the spiral pipe is 100 cm; the width of the coiled spiral pipe is 10cm, and the inner diameter of the cooling cavity is not smaller than 10 cm.
The invention also provides a tobacco product aerosol condensation, trapping and extraction method, which adopts a tobacco product aerosol condensation, trapping and extraction device, and the device comprises a trapping part, a cooling cavity and an elution part, wherein the trapping part comprises a spiral pipe, a first end pipe arranged at one end of the spiral pipe and a second end pipe arranged at one end of the spiral pipe, which is far away from the first end pipe; the elution part comprises a first branch pipe, a second branch pipe and a communicating pipe, wherein the first branch pipe is used for being communicated with the first end pipe after the aerosol is captured by the capture part, the second branch pipe is used for being communicated with the second end pipe after the aerosol is captured by the capture part, the communicating pipe is used for being communicated with the first branch pipe and the second branch pipe, a piston is arranged in the communicating pipe, a displacement platform which is parallel to the communicating pipe is arranged on the outer side of the communicating pipe, the displacement platform is connected with the piston through a connecting rod, and a sliding groove which is used for facilitating the connecting rod to slide is arranged on the side wall of the communicating pipe, so that the piston is driven to reciprocate in the communicating pipe when the displacement platform reciprocates;
the method comprises the following steps:
s1: pouring a condensing medium into the cooling cavity;
s2: inserting the spiral pipe of the trapping part into the cooling cavity and immersing the spiral pipe in a condensing medium, wherein a first end pipe and a second end pipe at two ends of the spiral pipe extend upwards to the upper part of the cooling cavity;
s3: the first end pipe and the second end pipe are respectively connected to the cigarette branch end and the smoking end of the smoking machine, the smoking machine is started to perform smoking, and the cigarettes with the first preset numerical value are continuously smoked;
s4: interchanging the positions of the cigarette branch end and the suction end of the smoking machine, starting the smoking machine to perform smoking, continuously smoking the cigarettes with the second preset numerical value, and finishing the trapping process;
s5: taking the trapping part out of the cooling cavity, adding a third preset numerical value ml of elution solvent ethanol into the trapping part, and hermetically connecting the first branch pipe and the second branch pipe of the elution part with the first end pipe and the second end pipe of the trapping part respectively;
s6: controlling a displacement platform of the elution part to perform reciprocating displacement to drive a piston to vibrate in a communicating pipe of the elution part in a reciprocating manner, extruding air to drive an elution solvent ethanol to perform primary elution on the inner side of the trapping part, stopping displacement of the displacement platform, disassembling the elution part, and guiding out an extraction liquid in the trapping part;
s7: and adding a fourth preset numerical value ml of elution solvent ethanol into the trapping part, connecting the eluting part, displacing the displacement platform again to carry out secondary elution on the inner side of the trapping part, and mixing the extract liquid generated for the second time with the extract liquid generated for the first time in the step S6 to finish the extraction process.
As a preferred aspect of the present invention, when step S1 is performed, the condensing medium is a mixture of powdered dry ice and isopropyl alcohol.
Preferably, when step S4 is executed, the second predetermined value is the same as the first predetermined value, and the sum of the first predetermined value and the second predetermined value is not greater than 30.
Preferably, in the step S2, the spiral pipe has an inner diameter of 8mm and a length of 100 cm; the width of the coiled spiral pipe is 10cm, and the inner diameter of the cooling cavity is not smaller than 10 cm.
As a preferable aspect of the present invention, when step S5 is executed, the third predetermined value is 5;
in step S7, the fourth predetermined value is 2.
The invention discloses a tobacco product aerosol condensation, trapping and extraction device and a trapping and extraction method, which have the beneficial effects that: simple structure can satisfy and make heating cigarette flue gas entrapment, can realize the extraction with the solvent as few as possible again, and then satisfies the external toxicity evaluation experiment requirement of novel tobacco products aerosol.
Drawings
FIG. 1 is a schematic structural diagram of a device for condensing, collecting and extracting an aerosol from a tobacco product according to the present invention;
FIG. 2 is a schematic structural diagram of a device for condensing, collecting and extracting an aerosol from smoke in the device for condensing, collecting and extracting the aerosol from tobacco products according to the present invention;
FIG. 3 is a schematic flow chart of a method for condensing, capturing and extracting aerosol of tobacco products according to the present invention;
FIG. 4 is a schematic diagram of the content of nicotine extracted under the inner diameters of different spiral curved tubes in the method for condensing, capturing and extracting the aerosol of tobacco products according to the present invention;
FIG. 5 is a schematic diagram illustrating the content of nicotine extracted at different temperatures in an aerosol condensation, trapping and extraction method for tobacco products according to the present invention;
FIG. 6 is a schematic diagram illustrating the nicotine content of a single cigarette extracted under different smoking counts in the method for condensing, capturing and extracting an aerosol of a tobacco product according to the present invention;
FIG. 7 is a schematic diagram illustrating the content of nicotine extracted by different elution methods in the method for condensing, capturing and extracting an aerosol of a tobacco product according to the present invention;
in the figure: 1. the device comprises a trapping part, 11, a spiral tube, 12, a first end tube, 13, a second end tube, 14, a holding rod, 2, a cooling cavity, 3, an elution part, 31, a first branch tube, 32, a second branch tube, 33, a communicating tube, 34, a piston, 35, a displacement platform, 36 and a connecting rod.
Detailed Description
The following are specific examples of the present invention and further describe the technical solutions of the present invention, but the present invention is not limited to these examples.
Various exemplary embodiments of the present invention will now be described in detail with reference to the accompanying drawings. It should be noted that: the relative arrangement of the modules and steps set forth in these embodiments and steps do not limit the scope of the invention unless specifically stated otherwise.
Meanwhile, it should be understood that the flows in the drawings are not merely performed individually for convenience of description, but a plurality of steps are performed alternately with each other.
The following description of at least one exemplary embodiment is merely illustrative in nature and is in no way intended to limit the invention, its application, or uses.
Techniques, methods, and systems known to those of ordinary skill in the relevant art may not be discussed in detail, but are intended to be part of the specification where appropriate.
The first embodiment is as follows: as shown in fig. 1 and 2, which are only one embodiment of the present invention, a condensation, trapping and extraction device for aerosol of tobacco products includes a trapping portion 1 for trapping aerosol generated during smoking of a cigarette, a temperature reduction chamber 2 for reducing the temperature of the trapping portion 1 when the trapping portion 1 traps the aerosol, and an elution portion 3 for extracting the aerosol in the trapping portion 1 after the trapping portion 1 traps the aerosol, where the trapping portion 1 includes a spiral tube 11, a first end tube 12 disposed at one end of the spiral tube 11, and a second end tube 13 disposed at one end of the spiral tube 11 far from the first end tube 12, a condensation medium is disposed in the temperature reduction chamber 2, and an inner diameter of the temperature reduction chamber 2 is not less than a length of the spiral tube 11, so that the first end tube 12 and the second end tube 13 at two ends of the spiral tube 11 are respectively connected to a smoking end and a cigarette branch end, and are inserted into the temperature reduction chamber 2 to condense smoke of the cigarette at the smoking end, thereby facilitating smoking Aerosol; elution portion 3 is including being used for behind the 1 entrapment aerosol of entrapment portion with first branch pipe 31 of first end pipe 12 intercommunication, be used for behind the 1 entrapment aerosol of entrapment portion with the second branch pipe 32 of second end pipe 13 intercommunication and be used for the intercommunication first branch pipe 31 and second branch pipe 32 communicating pipe 33, be provided with piston 34 in the communicating pipe 33, the communicating pipe 33 outside be provided with communicating pipe 33 parallel arrangement's displacement platform 35, displacement platform 35 through connecting rod 36 with piston 34 is connected, be provided with on the communicating pipe 33 lateral wall and be used for the convenience connecting rod 36 gliding spout for drive during displacement platform 35 reciprocating motion piston 34 is in reciprocating motion in communicating pipe 33.
In the invention, in order to collect aerosol in smoke when a smoking machine sucks cigarettes at a suction end, a collecting part 1 is arranged between the suction end and the cigarettes, the smoke passes through the collecting part 1 to collect the aerosol, the smoke needs to be condensed in order to collect the aerosol conveniently, and a spiral pipe 11 of the collecting part 1 is immersed into a cooling cavity 2 provided with a condensing medium to be condensed; and finally, the aerosol generated by complete condensation is sucked and remained in the spiral tube 11, the aerosol in the trapping part 1 is extracted through the elution part 3 and the elution solvent ethanol, so that the extraction liquid mixed with the smoke aerosol is obtained, and the subsequent in-vitro toxicology experiment evaluation is facilitated.
Firstly, the cooling part 2 is provided, the cooling cavity 2 is internally provided with a condensing medium, the condensing medium is a mixture of powdered dry ice and isopropanol, and can form a low temperature of-78 ℃ to cool the spiral pipe 11, and of course, the cooling cavity 2 is a heat insulation part, so that the temperature loss of the condensing medium is reduced as much as possible, and the guarantee of the condensation time is ensured.
Then is entrapment portion 1, entrapment portion 1 include spiral pipe 11, set up in the first end pipe 12 of spiral pipe 11 one end with set up in spiral pipe 11 keeps away from the second end pipe 13 of the one end of first end pipe 12, suction end and cigarette end are connected to first end pipe 12 and second end pipe 13 respectively and aspirate, and spiral pipe 11 sets up for taking place the area of contact of heat exchange with the condensation medium in increase and the cooling chamber 2.
Here, the inner diameter of the spiral pipe is 8mm, and the length is 100 cm; the width of the coiled spiral pipe is 10cm, and the inner diameter of the cooling cavity is not smaller than 10 cm. Like this the internal diameter in cooling chamber 2 is not less than the length of spiral pipe 11 for first end pipe 12 and the second end pipe 13 at spiral pipe 11 both ends insert when being connected to suction end and cigarette end respectively and insert to carry out the condensation with the flue gas of a cigarette in cooling chamber 2 and be convenient for suction end entrapment aerosol.
Before the flue gas aerosol entrapment that uses entrapment portion 1 to heat incombustible cigarette, be connected to the entrapment portion 1 on the electron cigarette smoking machine, also be that first end pipe 12 is connected to the smoking machine suction end, second end pipe 13 is connected to cigarette branch end, then with spiral pipe 11 submergence to the condensation medium in cooling chamber 2 in, and first end pipe 12 and second end pipe 13 stretch out to the condensation medium top, just begin to carry out the entrapment this moment, open the smoking machine and carry out a cigarette suction.
It should be noted that, in the trapping process, the suction end and the cigarette branch end of the smoking machine can be replaced and installed, and the same trapping operation is carried out again; namely, the smoking machine suction end is connected to the first end pipe, the cigarette end is connected to the second end pipe, and the first stage trapping is carried out; the smoking machine smoking end is then connected to the second end tube and the tobacco rod end is connected to the first end tube for a second stage of trapping.
And finally, the elution part 3 is also called as an extraction auxiliary part, the elution part 3 comprises a first branch pipe 31 which is used for being communicated with the first end pipe 12 after the trapping part 1 traps the aerosol, a second branch pipe 32 which is used for being communicated with the second end pipe 13 after the trapping part 1 traps the aerosol, and a communicating pipe 33 which is used for being communicated with the first branch pipe 31 and the second branch pipe 32, a piston 34 is arranged in the communicating pipe 33, a displacement platform 35 which is arranged in parallel with the communicating pipe 33 is arranged on the outer side of the communicating pipe 33, the displacement platform 35 is connected with the piston 34 through a connecting rod 36, and a sliding groove which is used for facilitating the sliding of the connecting rod 36 is arranged on the side wall of the communicating pipe 33, so that the piston 34 is driven to reciprocate in the communicating pipe 33 when the displacement platform 35 reciprocates.
Here, the displacement platform 35 is actually a movable block, and is driven by a driving machine to displace, and the displacement platform 35 drives the piston 34 to reciprocate in the communicating pipe 33 along the extending direction of the communicating pipe 33 through the connecting rod 36, so as to push the air in the communicating pipe 33 to squeeze the eluent in the trap part 1, and elute and extract the eluent in the trap part 1.
When elution is carried out, a certain amount of elution solvent ethanol is added into the trapping part 1, and the first branch pipe 31 and the second branch pipe 32 of the elution part 3 are respectively connected with the first end pipe 12 and the second end pipe 13 of the trapping part 1 in a sealing way; controlling the displacement platform 35 of the elution part 3 to carry out reciprocating displacement to drive the piston 34 to vibrate in a communicating pipe 33 of the elution part 3 in a reciprocating manner, and extruding air to drive the elution solvent ethanol to elute the inner side of the trapping part 1; after the elution is completed, the displacement platform 35 stops displacing, the elution part 3 is disassembled, and the extraction liquid in the trapping part 1 is led out, thereby completing the extraction process.
It should be noted that the elution process is also performed twice, and 5ml of ethanol as an elution solvent is used for the first elution; then using 2ml of elution solvent ethanol for secondary elution; and mixing the extraction liquid obtained by two times of elution to obtain the extraction liquid actually obtained in the whole capturing and extracting process.
Moreover, the volume of the communicating tube 33 is not less than twice of the volume of the spiral tube 11, that is, when the piston 34 performs a cycle of reciprocating motion, the blown air can push the elution solvent ethanol to flow to the whole trapping part 1 (including the spiral tube 11, the first end tube 12 and the second end tube 13), that is, the elution can be performed in all directions, and the aerosol at each position in the whole trapping part 1 can be extracted.
Finally, a holding rod 14 is connected between the first end tube 12 and the second end tube 13 to facilitate holding the entire trapping part 1 during both trapping and elution steps, and the holding rod 14 is typically held by a robot arm.
The condensation, trapping and extraction device for the aerosol of the tobacco products, disclosed by the invention, has a simple structure, can meet the trapping of smoke of heated cigarettes, can realize extraction by using a solvent as little as possible, and further meets the experimental requirements of in vitro toxicity evaluation of the aerosol of the novel tobacco products.
In a second embodiment, as shown in fig. 1 to 7, the present invention further provides a method for condensing, capturing and extracting an aerosol of a tobacco product in the above embodiment, which employs a device for condensing, capturing and extracting an aerosol of a tobacco product, the device including a capturing portion, a temperature-lowering chamber, and an eluting portion, wherein the capturing portion includes a spiral tube, a first end tube disposed at one end of the spiral tube, and a second end tube disposed at one end of the spiral tube away from the first end tube; the elution part comprises a first branch pipe, a second branch pipe and a communicating pipe, wherein the first branch pipe is used for being communicated with the first end pipe after the aerosol is captured by the capture part, the second branch pipe is used for being communicated with the second end pipe after the aerosol is captured by the capture part, the communicating pipe is used for being communicated with the first branch pipe and the second branch pipe, a piston is arranged in the communicating pipe, a displacement platform which is parallel to the communicating pipe is arranged on the outer side of the communicating pipe, the displacement platform is connected with the piston through a connecting rod, and a sliding groove which is used for facilitating the connecting rod to slide is arranged on the side wall of the communicating pipe, so that the piston is driven to reciprocate in the communicating pipe when the displacement platform reciprocates;
the method comprises the following steps:
s1: pouring a condensing medium into the cooling cavity;
s2: inserting the spiral pipe of the trapping part into the cooling cavity and immersing the spiral pipe in a condensing medium, wherein a first end pipe and a second end pipe at two ends of the spiral pipe extend upwards to the upper part of the cooling cavity;
s3: the first end pipe and the second end pipe are respectively connected to the cigarette branch end and the smoking end of the smoking machine, the smoking machine is started to perform smoking, and the cigarettes with the first preset numerical value are continuously smoked;
s4: interchanging the positions of the cigarette branch end and the suction end of the smoking machine, starting the smoking machine to perform smoking, continuously smoking the cigarettes with the second preset numerical value, and finishing the trapping process;
s5: taking the trapping part out of the cooling cavity, adding a third preset numerical value ml of elution solvent ethanol into the trapping part, and hermetically connecting the first branch pipe and the second branch pipe of the elution part with the first end pipe and the second end pipe of the trapping part respectively;
s6: controlling a displacement platform of the elution part to perform reciprocating displacement to drive a piston to vibrate in a communicating pipe of the elution part in a reciprocating manner, extruding air to drive an elution solvent ethanol to perform primary elution on the inner side of the trapping part, stopping displacement of the displacement platform, disassembling the elution part, and guiding out an extraction liquid in the trapping part;
s7: and adding a fourth preset numerical value ml of elution solvent ethanol into the trapping part, connecting the eluting part, displacing the displacement platform again to carry out secondary elution on the inner side of the trapping part, and mixing the extract liquid generated for the second time with the extract liquid generated for the first time in the step S6 to finish the extraction process.
In the present embodiment, when step S1 is executed, the condensing medium is a mixture of powdered dry ice and isopropyl alcohol.
Optimization for condensation temperature: the ice-water mixture, the pure powder dry ice and the dry ice isopropanol mixture are respectively used for carrying out smoke condensation and trapping, and the low temperatures which can be realized by the ice-water mixture, the pure powder dry ice and the dry ice isopropanol mixture are respectively 0 ℃, 78.5 ℃ and 78 ℃. The same cigarette number is sucked and trapped by an electronic smoking machine, and the best effect of the condensed smoke of the dry ice isopropanol mixture is obtained through experimental comparison, as shown in figure 5.
In this embodiment, when step S2 is executed, the inner diameter of the spiral pipe is 8mm, and the length is 100 cm; the width of the coiled spiral pipe is 10cm, and the inner diameter of the cooling cavity is not smaller than 10 cm.
Optimizing the size of the spiral condenser pipe: the same cigarettes are respectively sucked under the conditions of different inner diameters and the same length of the spiral condensation pipes, then the smoke condensate is extracted by the same solvent volume, the chromatographic analysis is carried out, the numerical values of the main chemical components of glycerin (VG), Propylene Glycol (PG) and Nicotine (NIC) are compared, and the inner diameter (8 mm) and the length (100 cm) of each spiral condensation pipe are finally obtained through a series of numerical comparison (the numerical values of the nicotine content are mainly compared), so that the optimal size of the device is shown in figure 4.
In the present embodiment, when step S4 is executed, the second predetermined value is the same as the first predetermined value, and the sum of the first predetermined value and the second predetermined value is not greater than 30.
Optimizing aiming at the number of smoking cigarettes: after the dimensions of the condenser tube were determined, the number of smoking cigarettes 5, 10, 15, 20, 25, 30, 35 were obtained, and as shown in fig. 6, it was found that when the number of smoking cigarettes trapped by the condenser tube was 30, the nicotine content per cigarette was the highest and was most similar to the result obtained by the standard method (filter trapping), and therefore the maximum number of cigarettes trapped by the condenser tube was 30.
Then the second predetermined value and the first predetermined value are both 15.
In the present embodiment, when step S5 is executed, the third predetermined value is 5; in step S7, the fourth predetermined value is 2.
Optimizing the volume of the extraction liquid: in order to ensure that the solvent can extract the condensate of the flue gas in the condensation pipe to the maximum extent, the condensation pipe is respectively flushed by using 7 ml of disposable solvent and 5ml + 2ml of solvent, and the obtained solution is subjected to chromatographic analysis on VG, PG and NIC values to obtain that the condensation pipe is eluted cleanly by using a 5ml + 2ml extraction method. (Note: 5ml + 2ml elution was performed by first using a 5ml extraction condenser and then washing the condenser with 2ml, and mixing the two solutions) as shown in FIG. 7.
The tobacco product aerosol condensation, trapping and extraction device and the trapping and extraction method have simple structures, can meet the trapping of the smoke of the heated cigarette, can realize extraction by using a solvent as less as possible, and further meet the experimental requirements of novel tobacco product aerosol in-vitro toxicity evaluation.
The present invention is not limited to the specific embodiments described, and various modifications and variations of the present invention are possible. Any modifications, equivalents, improvements and the like made to the above embodiments in accordance with the technical spirit of the present invention should be included in the scope of the present invention.
Claims (10)
1. A tobacco products aerosol condensation entrapment extraction element which characterized in that: including entrapment portion (1) that is used for entrapment cigarette aerosol that produces when smoking, be used for do during entrapment portion (1) entrapment aerosol cooling chamber (2) that lower the temperature and being used for are carried out in entrapment portion (1) extraction behind entrapment portion (1) entrapment aerosol elution portion (3) of aerosol in entrapment portion (1), entrapment portion (1) include spiral pipe (11), set up in first end pipe (12) of spiral pipe (11) one end with set up in spiral pipe (11) are kept away from second end pipe (13) of the one end of first end pipe (12), be provided with condensing medium in cooling chamber (2), just the internal diameter of cooling chamber (2) is not less than the length of spiral pipe (11) for first end pipe (12) and second end pipe (13) at spiral pipe (11) both ends insert when being connected to suction end and cigarette end respectively to be convenient for the flue gas of cigarette condenses in cooling chamber (2) (ii) a The elution part (3) comprises a first branch pipe (31) communicated with the first end pipe (12) after the collection part (1) collects the aerosol, a second branch pipe (32) communicated with the second end pipe (13) after the collection part (1) collects the aerosol, and a communicating pipe (33) communicated with the first branch pipe (31) and the second branch pipe (32), a piston (34) is arranged in the communicating pipe (33), a displacement platform (35) which is arranged in parallel with the communicating pipe (33) is arranged on the outer side of the communicating pipe (33), the displacement platform (35) is connected with the piston (34) through a connecting rod (36), a sliding groove for facilitating the sliding of the connecting rod (36) is arranged on the side wall of the communicating pipe (33), so that the piston (34) is driven to reciprocate in the communicating pipe (33) when the displacement platform (35) reciprocates.
2. A smoking article aerosol condensation capture extraction device according to claim 1, wherein: the condensing medium is a mixture of powdered dry ice and isopropanol.
3. A smoking article aerosol condensation capture extraction device according to claim 1, wherein: the cooling cavity (2) is a heat insulation piece.
4. A smoking article aerosol condensation capture extraction device according to claim 1, wherein: the volume of the communicating pipe (33) is not less than twice the volume of the spiral pipe (11).
5. A smoking article aerosol condensation capture extraction device according to claim 1, wherein: a holding rod (14) is connected between the first end tube (12) and the second end tube (13).
6. A tobacco product aerosol condensation, trapping and extraction method adopts a tobacco product aerosol condensation, trapping and extraction device which comprises a trapping part, a cooling cavity and an elution part, and is characterized by comprising the following steps:
s1: pouring a condensing medium into the cooling cavity;
s2: inserting the spiral pipe of the trapping part into the cooling cavity and immersing the spiral pipe in a condensing medium, wherein a first end pipe and a second end pipe at two ends of the spiral pipe extend upwards to the upper part of the cooling cavity;
s3: the first end pipe and the second end pipe are respectively connected to the cigarette branch end and the smoking end of the smoking machine, the smoking machine is started to perform smoking, and the cigarettes with the first preset numerical value are continuously smoked;
s4: interchanging the positions of the cigarette branch end and the suction end of the smoking machine, starting the smoking machine to perform smoking, continuously smoking the cigarettes with the second preset numerical value, and finishing the trapping process;
s5: taking the trapping part out of the cooling cavity, adding a third preset numerical value ml of elution solvent ethanol into the trapping part, and hermetically connecting the first branch pipe and the second branch pipe of the elution part with the first end pipe and the second end pipe of the trapping part respectively;
s6: controlling a displacement platform of the elution part to perform reciprocating displacement to drive a piston to vibrate in a communicating pipe of the elution part in a reciprocating manner, extruding air to drive an elution solvent ethanol to perform primary elution on the inner side of the trapping part, stopping displacement of the displacement platform, disassembling the elution part, and guiding out an extraction liquid in the trapping part;
s7: and adding a fourth preset numerical value ml of elution solvent ethanol into the trapping part, connecting the eluting part, displacing the displacement platform again to carry out secondary elution on the inner side of the trapping part, and mixing the extract liquid generated for the second time with the extract liquid generated for the first time in the step S6 to finish the extraction process.
7. A smoking article aerosol condensation capture extraction process according to claim 6, wherein:
in step S1, the condensing medium is a mixture of powdered dry ice and isopropyl alcohol.
8. A smoking article aerosol condensation capture extraction process according to claim 6, wherein:
in step S4, the second predetermined value is the same as the first predetermined value, and the sum of the first predetermined value and the second predetermined value is not greater than 30.
9. A smoking article aerosol condensation capture extraction process according to claim 6, wherein:
when the step S2 is executed, the inner diameter of the spiral pipe is 8mm, and the length of the spiral pipe is 100 cm; the width of the coiled spiral pipe is 10cm, and the inner diameter of the cooling cavity is not smaller than 10 cm.
10. A smoking article aerosol condensation capture extraction process according to claim 6, wherein:
when step S5 is executed, the third predetermined value is 5;
in step S7, the fourth predetermined value is 2.
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Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH05142110A (en) * | 1991-11-25 | 1993-06-08 | Matsushita Electric Ind Co Ltd | Sample elution method and device and sample measuring device |
| CN102636374A (en) * | 2012-04-18 | 2012-08-15 | 广东中烟工业有限责任公司 | Cold-trap gathering analytical method for volatile and semi-volatile matters in cigarette smoke |
| CN203490202U (en) * | 2013-09-23 | 2014-03-19 | 王飞 | High performance liquid chromatograph |
| CN205484222U (en) * | 2016-01-07 | 2016-08-17 | 刘广文 | Liquid chromatograph improves device |
| CN108051524A (en) * | 2018-01-05 | 2018-05-18 | 国家烟草质量监督检验中心 | A kind of cigarette, the assay method for heating Related Component in do not burn cigarette, electronic cigarette main flume |
| CN207816658U (en) * | 2018-01-05 | 2018-09-04 | 国家烟草质量监督检验中心 | A kind of smoking machine moisture of cigarette main stream smoke collection device |
| CN110988204A (en) * | 2019-11-07 | 2020-04-10 | 云南中烟工业有限责任公司 | Method for measuring migration volume of colorant smoke in filter tip by simulating cigarette smoking |
| CN111366666A (en) * | 2018-12-26 | 2020-07-03 | 贵州中烟工业有限责任公司 | In-situ extraction method for particulate matter components of main stream smoke of cigarettes |
| CN211042858U (en) * | 2019-11-18 | 2020-07-17 | 国电南京电力试验研究有限公司 | SO in flue gas of thermal power plant3Sampling device |
| CN212341128U (en) * | 2020-06-02 | 2021-01-12 | 成都和合医学检验所有限公司 | Liquid chromatograph sampling device |
-
2021
- 2021-11-02 CN CN202111285468.6A patent/CN114002047B/en active Active
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH05142110A (en) * | 1991-11-25 | 1993-06-08 | Matsushita Electric Ind Co Ltd | Sample elution method and device and sample measuring device |
| CN102636374A (en) * | 2012-04-18 | 2012-08-15 | 广东中烟工业有限责任公司 | Cold-trap gathering analytical method for volatile and semi-volatile matters in cigarette smoke |
| CN203490202U (en) * | 2013-09-23 | 2014-03-19 | 王飞 | High performance liquid chromatograph |
| CN205484222U (en) * | 2016-01-07 | 2016-08-17 | 刘广文 | Liquid chromatograph improves device |
| CN108051524A (en) * | 2018-01-05 | 2018-05-18 | 国家烟草质量监督检验中心 | A kind of cigarette, the assay method for heating Related Component in do not burn cigarette, electronic cigarette main flume |
| CN207816658U (en) * | 2018-01-05 | 2018-09-04 | 国家烟草质量监督检验中心 | A kind of smoking machine moisture of cigarette main stream smoke collection device |
| CN111366666A (en) * | 2018-12-26 | 2020-07-03 | 贵州中烟工业有限责任公司 | In-situ extraction method for particulate matter components of main stream smoke of cigarettes |
| CN110988204A (en) * | 2019-11-07 | 2020-04-10 | 云南中烟工业有限责任公司 | Method for measuring migration volume of colorant smoke in filter tip by simulating cigarette smoking |
| CN211042858U (en) * | 2019-11-18 | 2020-07-17 | 国电南京电力试验研究有限公司 | SO in flue gas of thermal power plant3Sampling device |
| CN212341128U (en) * | 2020-06-02 | 2021-01-12 | 成都和合医学检验所有限公司 | Liquid chromatograph sampling device |
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