CN113995826A

CN113995826A - External use infusion for treating onychomycosis by traditional Chinese medicine combination and preparation method thereof

Info

Publication number: CN113995826A
Application number: CN202111388635.XA
Authority: CN
Inventors: 石彬; 张世林; 石贵花; 石之州
Original assignee: Individual
Current assignee: Individual
Priority date: 2021-11-22
Filing date: 2021-11-22
Publication date: 2022-02-01

Abstract

The invention relates to the field of traditional Chinese medicines, and in particular relates to an external infusion for treating onychomycosis by a traditional Chinese medicine composition and a preparation method thereof, wherein the external infusion comprises the following components in parts by weight: 40-50 parts of tail wine, 2-10 parts of ginger juice and 2-5 parts of green pepper juice. The preparation method comprises the following steps: mixing the tail wine, rhizoma Zingiberis recens juice and green fructus Zanthoxyli juice at a certain proportion, standing for 18-24 hr, and filtering to obtain the topical infusion. The external use infusion provided by the invention contains the tail wine, the ginger juice and the green pepper juice, the tail wine is used as a main component, the tail wine fermented by grains can be secondarily utilized, the utilization efficiency of the tail wine is improved, the organic components in the tail wine can dissolve the effective substances in the green pepper juice and the ginger juice so as to improve the leaching rate of the effective components, and the ethanol in the tail wine has the disinfection and sterilization effects and can disinfect and sterilize diseased nails.

Description

External use infusion for treating onychomycosis by traditional Chinese medicine combination and preparation method thereof

Technical Field

The invention relates to the field of traditional Chinese medicines, and particularly relates to an external use infusion for treating onychomycosis by combining traditional Chinese medicines and a preparation method thereof.

Background

Onychomycosis refers to a disease caused by dermatophyte invading the nail plate or under the nail, is a mycosis of nails of fingers (toes) of hands and feet, often occurs after tinea manus and pedis, and can also occur simultaneously, is commonly called as 'onychomycosis', and is similar to 'goose paw wind' and 'greasy onychomycosis' in the traditional Chinese medicine. The nail usually turns color, loses luster, thickens and becomes brittle, uneven, brown or grey white, and the heavy nail plate is separated from the nail bed. Onychomycosis is an infection of the nail caused by fungi such as dermatophytes, yeasts and non-dermatophytes. Onychomycosis is common: fungal onychomycosis (superficial onychomycosis), a type of lesion limited to one piece of the nail or its tip; the onychomycosis includes distal lateral type, proximal hyponychium type and superficial white type, and the lesions start from both sides or distal end of the nail, and then the hyponychium is infected.

The current treatment means include surgical nail removal, oral medicine and external therapy. The operative nail pulling is suitable for single-shot nails, and the method has large wound surface, bleeding and easy infection. The postoperative patient generally feels more pain, is easy to re-infect and relapse, and is rarely used clinically. Patients with heart disease, hypertension, diabetes, etc. are not suitable for surgical treatment. The oral administration medicine is suitable for a plurality of nails (toes), for example, 250mg of terbinafine tablets are orally taken every day for 6-12 weeks continuously; 400 mg/day of itraconazole, continuously taking for 7 days, and taking a rest for 21 days as a treatment course, wherein the treatment course lasts for 3-6 treatment courses; 150mg of fluconazole is taken 1-2 times a week for more than 4 months continuously. Can be used for treating more than 80% of onychomycosis and onychomycosis. However, because the medicines can play an antibacterial role only by reaching the deck where fungi are parasitized, the dosage is large, the administration time is long, and the adverse reaction of the medicines is monitored regularly. The external therapy mainly comprises local application, package cutting, etc., such as grinding irregular bad nails with nail file, preferably once every 2 days; then applying 30% glacial acetic acid or 10% glacial acetic acid on the affected part A for 1 time per day for more than 3-6 months. If the diseased nails are thinned before the application, the curative effect is better. Care should be taken to protect the periungual skin with mild ointment prior to nail application; or topical antifungal agents such as terbinafine tincture, amorolfine liniment, ciclopirox olamine ketone liniment, etc.

Terbinafine and itraconazole are orally taken to treat onychomycosis, although the clinical curative effect is good, in 2002, the U.S. food and drug administration gives a warning to alert the risk of serious adverse drug reactions related to itraconazole and terbinafine, which indicates that the two drugs may cause serious liver function damage, liver failure and even death, and itraconazole may also cause congestive heart failure. Meanwhile, the elderly, children and patients with liver and kidney insufficiency are not suitable for systemic medication.

Therefore, how to develop a traditional Chinese medicine preparation for treating onychomycosis becomes an urgent problem to be solved in the prior art.

Disclosure of Invention

The invention aims to provide an external Chinese medicinal composition infusion for treating onychomycosis, which contains tail wine, green zanthoxylum juice and ginger juice, can disinfect and sterilize diseased nails, has the efficacy of relieving itching and can cure onychomycosis.

The invention also aims to provide a preparation method of the external-use infusion for treating onychomycosis by combining traditional Chinese medicines, which is characterized in that the tail wine obtained by fermenting the grain grains is directly mixed with the ginger juice and the green pepper juice, the preparation method is simple, the requirements on the preparation environment and equipment are low, and the external-use infusion can be produced in large scale and can be prepared by self.

The technical problem to be solved by the invention is realized by adopting the following technical scheme.

On one hand, the embodiment of the invention provides an external use infusion for treating onychomycosis by combining traditional Chinese medicines, which comprises the following components in parts by weight: 40-50 parts of tail wine, 2-10 parts of ginger juice and 2-5 parts of green pepper juice.

On the other hand, the embodiment of the invention provides a preparation method of the externally applied traditional Chinese medicine infusion for treating onychomycosis, which comprises the steps of uniformly mixing the tail wine, the ginger juice and the green pepper juice according to a proportion, standing for 18-24 hours and filtering.

Compared with the prior art, the embodiment of the invention has at least the following advantages or beneficial effects:

the external use infusion provided by the invention contains the tail wine, the ginger juice and the green pepper juice, the tail wine is used as a main component, the tail wine fermented by grains can be secondarily utilized, the utilization efficiency of the tail wine is improved, in addition, the tail wine contains rich organic components such as acid, ester, alcohol, aldehyde, ketone and the like, compared with alcohol, the components in the tail wine are more rich, the ginger juice and the green pepper juice are added into the tail wine, the organic components in the tail wine can dissolve effective substances in the green pepper juice and the ginger juice, the leaching rate of the effective components is improved, the content of the effective components dissolved in the tail wine is high, and the effective components can better enter pores of skin to deeply disinfect the pores to treat onychomycosis; on the other hand, the ethanol in the tail wine has the function of disinfection and sterilization, and can disinfect and sterilize diseased nails.

The green pricklyash peel juice contains a large amount of limonene, and the limonene can inhibit the propagation of staphylococcus aureus, aspergillus niger and escherichia coli. The compound is matched with the tail wine to realize synergistic interaction, so that the sterilization effect is further improved.

The ginger juice mainly contains zingerone, gingerol and gingerol, and has certain volatility. The ginger juice is added into the tail wine and applied to the treatment of onychomycosis, and in the treatment process, the zingerone, the gingerol and the gingerol in the ginger juice can enhance and accelerate the blood circulation of the affected skin position, so that the skin can absorb the effective components in the ginger juice and the green pepper juice more easily, and the treatment effect of the external infusion is improved.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.

It should be noted that the embodiments and features of the embodiments may be combined with each other without conflict. The present invention will be described in detail below with reference to specific examples.

In the invention, 40-50 parts of the tail wine is used as a main component, the tail wine contains rich organic components such as acid, ester, alcohol, aldehyde, ketone and the like, not only has excellent disinfection and sterilization effects, but also can be used as an organic solvent to dissolve the effective components in green pricklyash peel juice and ginger juice so as to improve the leaching rate of the effective components, and the effective components dissolved in the tail wine can better enter pores of skin to deeply disinfect and sterilize the pores so as to treat onychomycosis.

The main component of the green pricklyash peel juice is limonene accounting for 25.10% of the total oil, and the limonene can inhibit the propagation of staphylococcus aureus, aspergillus niger and escherichia coli. Limonene is a safe and nontoxic bacteriostatic substance, and the limonene is accumulated on the surface of a microorganism, so that the content of unsaturated fatty acid in the membrane is greatly reduced, the components of the membrane are changed or the integrity of the membrane is damaged, and the proton power can be reduced, thereby achieving the bacteriostatic action.

Also contains 21.79% of 1, 8-cineole, and the cineole can be used in combination with other medicines for treating influenza, common cold, bacillary dysentery, enteritis, various infections (including parotitis, epidemic cerebrospinal meningitis, suppurative tonsillitis, infantile head sore, erysipelas, and traumatic infection), pulmonary tuberculosis, and various dermatoses.

Also contains 11.99% of myrcene, and small amount of a-and beta-pinene, sabinene, beta-phellandrene, beta-ocimene-X, p-cymene, alpha-terpinene, perillene, linalool, 4-terpinenic acid, efletin, alpha-terpineol, trans-caryophyllene, terpineol acetate, humulene, nerol acetate, beta-piper cubene, geraniol acetate, nerolidol isomer, etc. The pericarp also contains vanilline, skimmianine, ruthenamine, 2' -hydroxy-N-isobutyl (2E,6E,8E,10E) -dodecenamide, and paprika is N-methyl-2-heptyl-4-quinolinone, dehydroentericin, nonacosane, etc.

The ginger juice mainly contains zingerone, gingerol and gingerol, and has certain volatility. Has effects in promoting blood circulation, stimulating gastric secretion, promoting digestion, and invigorating stomach. The ginger juice is added into the tail wine and applied to treating onychomycosis, and in the treatment process, the zingerone, the gingerol and the gingerol in the ginger juice can enhance and accelerate the blood circulation of the affected skin position, so that the skin can absorb the effective components in the ginger juice and the green prickleyash juice more easily, and the treatment effect of the external use infusion is improved.

In some embodiments of the present invention, the external use infusion for onychomycosis comprises the following components in parts by weight: 45 parts of tail wine, 7 parts of ginger juice and 3 parts of green pepper juice. Under the proportion, after the external use infusion is used for a long time by a patient, the treatment effect on onychomycosis is good, and meanwhile, the external use infusion has small irritation to skin and can be used for a long time.

In some embodiments of the present invention, the tail wine is one of a glutinous rice tail wine, a rice tail wine and a sorghum tail wine.

On the other hand, the embodiment of the invention provides a preparation method of the externally applied traditional Chinese medicine infusion for treating onychomycosis, which comprises the steps of uniformly mixing the tail wine, the ginger juice and the green pepper juice according to a proportion, standing for 18-24 hours and filtering. In the process of placing, the effective components in the ginger and the pepper can be fully dissolved in the tail liquor, so that the leaching rate of the effective components is improved, the content of the effective components in the tail liquor is high, and the treatment effect of the external infusion is improved.

In some embodiments of the invention, the method of preparing the tail wine comprises the steps of:

s1 soaking grains, steaming, spreading and airing;

s2 adding distiller' S yeast, sealing and fermenting;

s3 distilling, collecting the first fermented wine, fermenting the wine dregs, and distilling to collect the tail wine.

In some embodiments of the present invention, in the step S1, the cooking temperature is 90-120 ℃.

In some embodiments of the invention, in the step S2, the mass ratio of the distiller' S yeast to the grain is (0.5-1.5): 10.

In some embodiments of the present invention, in the step S2, the sealed fermentation time is 2-3 months.

In some embodiments of the invention, the ginger juice is prepared by: selecting fresh ginger, cleaning, adding the soaking solution, mixing uniformly, adding into a juicer, juicing, filtering, concentrating, and collecting juice.

In some embodiments of the present invention, the green pepper juice is prepared by the following steps: mixing fresh fructus Zanthoxyli with solvent, soaking for 3-4 days, adding into crusher, crushing, filtering, concentrating, and collecting juice.

The features and properties of the present invention are described in further detail below with reference to examples.

Example 1

Preparing the tail wine

Soaking Oryza Glutinosa in cold water for 24 hr, changing water every 6 hr, steaming at 100 + -2 deg.C, and spreading for air drying. When the temperature of the sticky rice is reduced to 25 +/-1 ℃, mixing the components in a mass ratio of 1: 10 mixing with distiller's yeast. Putting the mixed distiller's yeast clinker into a barrel, covering with a layer of preservative film, then covering with a layer of plastic cloth, binding with an elastic band, standing and fermenting for 2 months. After the first fermentation wine in the collecting barrel, the wine residue is continuously fermented for 2 months, and the wine residue is collected to be the tail wine.

Preparing ginger juice

Cleaning fresh ginger, slicing, adding water, placing in a container, soaking for 2 hr, adding water and ginger in the container into a juicer, squeezing to obtain juice, filtering with filter cloth, collecting juice, and concentrating at 30 deg.C until the water content is 10 wt% to obtain ginger juice.

Preparing green pricklyash peel juice

Mixing fresh fructus Zanthoxyli with ethanol water solution, soaking for 3 days, adding fructus Zanthoxyli and solvent into a crusher, crushing, filtering, collecting juice, and concentrating at 30 deg.C until water content is 10 wt% to obtain green fructus Zanthoxyli juice.

Preparation of an infusion for external use

Weighing 450g of tail wine, 70g of ginger juice and 30g of green pepper juice, uniformly mixing, standing for 18h, stirring 1 time every 1h in the standing process, stirring for 10min every time, standing for 18h, and then filtering to obtain the external infusion.

Example 2

Preparing the tail wine

Soaking rice in cold water for 24 hr, changing water every 7 hr, steaming at 110 + -2 deg.C, and spreading for air drying. When the temperature of the rice is reduced to 25 +/-1 ℃, the weight ratio of the rice to the water is 1.5: 10 mixing with distiller's yeast. Putting the mixed distiller's yeast clinker into a barrel, covering with a layer of preservative film, then covering with a layer of plastic cloth, binding with an elastic band, standing and fermenting for 2 months. After the first fermentation wine in the collecting barrel, the wine residue is continuously fermented for 2 months, and the wine residue is collected to be the tail wine.

Preparing ginger juice

Cleaning fresh ginger, slicing, adding water, placing in a container, soaking for 1.5 hr, adding water and ginger in the container into a juicer, squeezing to obtain juice, filtering with filter cloth, collecting juice, and concentrating at 30 deg.C until the water content is 15 wt% to obtain ginger juice.

Preparing green pricklyash peel juice

Mixing fresh fructus Zanthoxyli with ethanol water solution, soaking for 4 days, adding fructus Zanthoxyli and solvent into a crusher, crushing, filtering, collecting juice, and concentrating at 30 deg.C until water content is 15 wt% to obtain green fructus Zanthoxyli juice.

Preparation of an infusion for external use

Weighing 500g of tail wine, 20g of ginger juice and 50g of green pepper juice, uniformly mixing, standing for 20h, stirring 1 time every 1h in the standing process, stirring for 10min every time, standing for 20h, and then filtering to obtain the external infusion.

Example 3

Preparing the tail wine

Soaking sorghum in cold water for 24 hr, changing water every 6 hr, steaming at 90 + -2 deg.C, and spreading for air drying. When the temperature of the sorghum is reduced to 25 +/-1 ℃, the mass ratio of the sorghum to the sorghum is 0.5: 10 mixing with distiller's yeast. Putting the mixed distiller's yeast clinker into a barrel, covering with a layer of preservative film, then covering with a layer of plastic cloth, binding with an elastic band, standing and fermenting for 3 months. After the first fermentation wine in the collecting barrel, the wine residue is continuously fermented for 3 months, and the wine is collected, namely the tail wine.

Preparing ginger juice

Cleaning fresh ginger, slicing, adding water, placing in a container, soaking for 3 hr, adding water and ginger in the container into a juicer, squeezing to obtain juice, filtering with filter cloth, collecting juice, and concentrating at 30 deg.C until the water content is 10 wt% to obtain ginger juice.

Preparing green pricklyash peel juice

Preparation of an infusion for external use

Weighing 400g of tail wine, 100g of ginger juice and 20g of green pepper juice, uniformly mixing, standing for 24h, stirring 1 time every 1h in the standing process, stirring for 10min every time, standing for 24h, and then filtering to obtain the external infusion.

Example 4

The tail wine, the ginger juice and the green pepper juice prepared in the example 1 are adopted to prepare the external infusion according to the following mixture ratio:

weighing 480g of tail wine, 50g of ginger juice and 50g of green pepper juice, uniformly mixing, standing for 24h, stirring 1 time every 1h in the standing process, stirring for 10min every time, standing for 24h, and then filtering to obtain the external infusion.

Example 5

weighing 500g of tail wine, 90g of ginger juice and 30g of green pepper juice, uniformly mixing, standing for 20h, stirring 1 time every 1h in the standing process, stirring for 10min every time, standing for 20h, and then filtering to obtain the external infusion.

Example 6

The tail wine prepared in example 1, the ginger juice prepared in example 2 and the green pepper juice are used to prepare an external infusion according to the following mixture ratio:

weighing 420g of tail wine, 70g of ginger juice and 40g of green pepper juice, uniformly mixing, standing for 24h, stirring 1 time every 1h in the standing process, stirring for 10min every time, standing for 24h, and then filtering to obtain the external infusion.

Examples of the experiments

1. Determination of MIC for infectious Strain

The test drugs were prepared by using the infusion solutions of examples 1 to 6, and the Minimum Inhibitory Concentrations (MIC) of the drugs against Staphylococcus aureus, Escherichia coli, Shigella dysenteriae, Candida albicans, Proteus, and Salmonella typhi were determined by tube dilution.

The liquid culture medium was added to 1.0mL of each tube with different concentrations of the drug solutions to give final concentrations of 800.00, 400.00, 200.00, 100.00, 50.00, 25.00, 12.50, 6.25, and 3.13mg/mL, respectively, and a final total volume of 1mL for each tube. Additionally, a normal control containing equal amount of MH liquid culture medium without any drug and a positive control containing bacteria and MH liquid culture medium without any drug are arranged. The concentration of each experimental tube is 2 multiplied by 10⁷CFU/mL of fresh culture of each strain in logarithmic growth phase was 0.1mL, and the culture was incubated at 37 ℃ for 24 hours for observation. And (3) culturing the candida albicans in a culture environment at 28 ℃ for 48h to observe the result, and observing the growth conditions of different strains by naked eyes, wherein the highest dilution of the medicine without bacterial growth is MIC. The results are shown in Table 1.

TABLE 1 minimum inhibitory concentrations (mg/mL) of the topical infusions of examples 1-6

As can be seen from Table 1 above, the minimum inhibitory concentrations of the infusions for external use of examples 1 to 6 were low, wherein the minimum inhibitory concentration for Staphylococcus aureus was 6.25mg/mL, the minimum inhibitory concentration for Escherichia coli was 12.5mg/mL, the minimum inhibitory concentration for Proteus was 25mg/mL, the minimum inhibitory concentration for Salmonella typhi was 25mg/mL, the minimum inhibitory concentration for Shigella dysenteriae was 50mg/mL, and the minimum inhibitory concentration for Candida albicans was 25 mg/mL. It can be shown that the infusions for external use of examples 1 to 6 of the present invention have excellent sterilizing efficacy and good sterilizing effect even at low concentration. The external use infusion is used for treating onychomycosis, and has a sterilization effect by adopting a soaking or spraying mode.

2. Determination of intestinal infection strain bacteriostatic diameter

The test drugs were the external infusions of examples 1 to 6, and the control drugs were ampicillin, norfloxacin and fluconazole, respectively, to determine the inhibitory diameters of Staphylococcus aureus, Escherichia coli, Shigella dysenteriae, Candida albicans, Proteus and Salmonella typhi.

The agar diffusion method (tube-disc method) was used: 2mL of nutrient agar which is melted and is kept at a constant temperature of about 58 ℃ and 0.1mL of logarithmic phase bacterial liquid (the concentration of the bacterial liquid is 1 multiplied by 10) are respectively added into a sterile test tube⁷CFU/mL), mixing, pouring into prepared plate, spreading, condensing, adding 3 oxford cups with diameter of 6mm, transferring 200 μ L of test solution with quantitative pipettor, repeating for 2 times, making parallel holes for 3 times, and setting positive drug control. After culturing for 24h in an incubator at 37 ℃, the diameters of two vertical directions of each inhibition zone are respectively measured by a vernier caliper, and the edge of the inhibition zone is limited to the limit that bacteria can not obviously grow by naked eyes. The results are shown in Table 2.

TABLE 2 bacteriostatic diameter (mm) of each drug

Staphylococcus aureus

Escherichia coli

Proteobacteria

Salmonella typhi

Dysentery bacillus

Candida albicans

Example 1

20.72±0.14⁺

18.35±0.10⁺

16.44±0.14⁺

16.00±0.11⁺

17.37±0.14⁺

15.89±0.17⁺

Example 2

21.56±0.13⁺

17.89±0.11⁺

15.34±0.14⁺

16.12±0.11⁺

17.02±0.15⁺

15.78±0.16⁺

Example 3

20.23±0.11⁺

17.56±0.10⁺

16.12±0.16⁺

16.89±0.12⁺

16.89±0.13⁺

15.02±0.13⁺

Example 4

21.15±0.14⁺

18.12±0.13⁺

16.79±0.17⁺

17.02±0.11⁺

16.78±0.14⁺

15.14±0.12⁺

Example 5

20.89±0.14⁺

17.02±0.15⁺

17.32±0.12⁺

17.02±0.12⁺

15.24±0.18⁺

Example 6

20.56±0.14⁺

17.78±0.14⁺

16.12±0.12⁺

16.56±0.11⁺

17.13±0.12⁺

15.69±0.17⁺

Ampicillin

27.21±0.14

25.75±0.13

24.79±0.14

25.75±0.13

23.48±0.16

15.89±0.23⁺

Norfloxacin hydrochloride

25.27±0.11

26.00±0.10

26.14±0.13

26.08±0.14

25.65±0.12

/

Fluconazole

/

26.00±0.12

Note: compared with norfloxacin⁺P<0.05。

As can be seen from the above Table 2, the external infusions of examples 1 to 6 have good bacteriostatic effects on Staphylococcus aureus, Escherichia coli, Bacillus dysenteriae, Candida albicans, Bacillus proteus and Salmonella typhi, and are superior to conventional commercially available bactericidal agents. When the external preparations of examples 1 to 6 were used for treating onychomycosis, the affected part was deeply sterilized by the external preparations to inhibit the propagation of various germs, thereby treating the onychomycosis.

3. Effect on Candida albicans

The test article was diluted with M-H liquid medium (liquid medium of Sabouraud's dextrose for Candida albicans) using a 2-fold sequential broth dilution method. Taking 10 clean sterile test tubes, adding 2.0mL of M-H broth (the first tube is 2.0mL of a test sample stock solution) into each test tube except the first tube, adding 2.0mL of 1/2 (the final concentration of the test sample and the M-H broth after being uniformly mixed) into the second tube, adding 2.0mL of the third tube into the third tube, then adding 2.0mL of the third tube into the fourth tube … …, and so on to ensure that the final concentration of the experimental drugs in each tube is 1 & lt/EN & gt1. 1/2, 1/4, 1/8, 1/16, 1/32, 1/64 and 1/128 positive control (no bacteria added with the test article) and negative control (no bacteria added with the test article added with the added bacteria added with the test article added with the added bacteria added with the added. The concentration of each experimental tube is 1 multiplied by 10⁷The growth condition of each strain of CFU/mL of the culture in the logarithmic growth phase is observed after 0.1mL of the culture in the logarithmic growth phase of each strain is cultured for 18-24h (the culture is carried out for 36-48h at 25-28 ℃ of Candida albicans) at 36 +/-1 ℃, and the highest dilution of the drug without bacterial growth is taken as the Minimum Inhibitory Concentration (MIC) of the drug. The results are shown in Table 3.

Table 3 minimum inhibitory concentrations of topical infusions of example 1

As can be seen from table 3, the external infusion of example 1 has a certain inhibitory effect on escherichia coli, staphylococcus aureus, pseudomonas aeruginosa and candida albicans, wherein the inhibitory effect on fungi (candida albicans) is superior to that of bacteria (escherichia coli, staphylococcus aureus and pseudomonas aeruginosa).

In conclusion, the Chinese medicinal preparation is prepared by mixing the glutinous rice tail wine, the sorghum tail wine, the ginger juice and the green pepper juice, and the external-use infusion has the effects of resisting bacteria and relieving itching and has better curative effect on onychomycosis. Limonene and 1, 8-cineole in the green pepper juice, and zingerone, gingerol and gingerol in the ginger juice are synergistic with the tail wine, so that blood circulation of affected skin positions is enhanced and accelerated, propagation of staphylococcus aureus, aspergillus niger and escherichia coli is inhibited, and onychomycosis can be effectively treated.

The embodiments described above are some, but not all embodiments of the invention. The detailed description of the embodiments of the present invention is not intended to limit the scope of the invention as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Claims

1. The externally-applied traditional Chinese medicine infusion for treating onychomycosis is characterized by comprising the following components in parts by weight:

40-50 parts of tail wine, 2-10 parts of ginger juice and 2-5 parts of green pepper juice.

2. The external use infusion for treating onychomycosis by combination of traditional Chinese medicines according to claim 1, which is characterized by comprising the following components in parts by weight:

45 parts of tail wine, 7 parts of ginger juice and 3 parts of green pepper juice.

3. The topical infusion of a combination of traditional Chinese medicines for treating onychomycosis according to claim 1, wherein the cocktail is one of a glutinous rice cocktail, a rice cocktail, and a sorghum cocktail.

4. A preparation method of the external use infusion for treating onychomycosis with the traditional Chinese medicine composition according to any one of claims 1 to 3, which is characterized by comprising the steps of uniformly mixing the tail wine, the ginger juice and the green pepper juice according to a proportion, standing for 18 to 24 hours and filtering.

5. The preparation method of the external use infusion for treating onychomycosis by combining traditional Chinese medicines according to claim 4, wherein the preparation method of the tail wine comprises the following steps:

s1 soaking grains, steaming, spreading and airing;

s2 adding distiller' S yeast, sealing and fermenting;

6. The method for preparing the external use infusion for treating onychomycosis according to claim 5, wherein the cooking temperature in step S1 is 90-120 ℃.

7. The method for preparing the external infusion for treating onychomycosis by combining traditional Chinese medicines according to claim 5, wherein the mass ratio of the distiller' S yeast to the grains in step S2 is (0.5-1.5): 10.

8. The method for preparing the external use infusion for treating onychomycosis with combination of traditional Chinese medicines according to claim 5, wherein in the step S2, the sealed fermentation time is 2-3 months.

9. The preparation method of the external use infusion for treating onychomycosis with the traditional Chinese medicine composition according to claim 4, wherein the ginger juice is prepared by the following steps: selecting fresh ginger, cleaning, adding the soaking solution, mixing uniformly, adding into a juicer, juicing, filtering, concentrating, and collecting juice.

10. The preparation method of the external use infusion for treating onychomycosis by combining the traditional Chinese medicines according to claim 4, wherein the green zanthoxylum bungeanum juice is prepared by the following steps: mixing fresh fructus Zanthoxyli with solvent, soaking for 3-4 days, adding into crusher, crushing, filtering, concentrating, and collecting juice.