CN113994952A

CN113994952A - Tumor cell temporary storage device capable of isolating external bacteria

Info

Publication number: CN113994952A
Application number: CN202111365864.XA
Authority: CN
Inventors: 朱晓菊; 李梦侠; 熊艳丽
Original assignee: Chinese Peoples Liberation Army Army Specialized Medical Center
Current assignee: Chinese Peoples Liberation Army Army Specialized Medical Center
Priority date: 2021-11-18
Filing date: 2021-11-18
Publication date: 2022-02-01

Abstract

The invention relates to the technical field of temporary cell storage, in particular to a temporary tumor cell storage device capable of isolating external bacteria, which comprises a storage box, wherein a storage cavity is formed in the storage box, a sterilization solution is injected into the storage cavity, an end cover is arranged at the top end of the storage box, a placement frame is placed in the storage cavity, a storage test tube is placed on the placement frame, a tube opening sealing device is arranged at the tube opening of the storage test tube and comprises a sleeve column, a sealing rubber sleeve and a sealing cover, the sleeve column is sleeved on the outer side of the tube opening of the storage test tube, the sealing rubber sleeve is covered at the top end of the sleeve column, the sealing cover is in threaded connection with the top end of the sleeve column, and the sealing cover is covered on the outer side of the top end of the storage test tube. According to the invention, the placing frame provided with the storage test tube is placed into the storage box filled with the sterilizing solution, so that the whole storage test tube can be soaked in the sterilizing solution after the end cover is arranged, tumor cells stored in the storage test tube are isolated from the external sterile environment, and the stored tumor cells are prevented from being polluted.

Description

Tumor cell temporary storage device capable of isolating external bacteria

Technical Field

The invention relates to the technical field of temporary cell storage, in particular to a temporary tumor cell storage device capable of isolating external bacteria.

Background

The tumor is characterized in that under the action of tumorigenic factors, genes of cells are changed, the normal regulation and control of the growth of the cells are lost, abnormal proliferation is caused, tumor cells are main components of the tumor and have tissue source specificity, after the tumor cells are sampled from the tumor tissues, samples need to be detected and analyzed, and when the detection and analysis cannot be timely performed after the tumor cells are sampled, the samples of the tumor cells need to be temporarily stored in a sterile test tube. Because when tumor cell is in the time keeping, external bacterium adheres to the test tube easily to lead to the bacterium to pollute tumor cell who keeps in easily, can cause the influence to tumor cell's detection and analysis result. In view of the above, we propose a temporary tumor cell buffer device capable of isolating external bacteria.

Disclosure of Invention

The present invention is directed to a temporary tumor cell storage device capable of isolating external bacteria, so as to solve the above problems in the prior art.

In order to achieve the purpose, the invention provides the following technical scheme:

the utility model provides a can completely cut off external bacterium's tumour cell temporary storage device, includes and stores the box, storage chamber has been seted up in the storage box, it has the bactericidal solution to inject into in the storage chamber, the top of storing the box is equipped with the end cover, the rack has been placed in the storage chamber, place on the rack and store the test tube, the mouth of pipe department of storing the test tube is provided with mouth of pipe sealing device, mouth of pipe sealing device includes the socle, seals gum cover and sealed lid, the socle cover is established store the orificial outside of test tube, sealed gum cover is established on the top of socle, sealed lid threaded connection is on the top of socle, and sealed lid cover is established in the outside on storage test tube top.

Preferably, the storage box is provided with a lug integrally formed on the left and right end surfaces of the top of the storage box, the lug is provided with a threaded hole, the left and right end surfaces of the end cover are provided with a convex plate integrally formed on the left and right end surfaces of the end cover, the convex plate is provided with a screwing handle, a threaded column is embedded at the bottom end of the screwing handle, and the threaded column penetrates through the convex plate and is in threaded connection with the threaded hole.

Preferably, the inner walls of the left side and the right side of the storage cavity are provided with slots, the top slot of each slot is provided with a guide groove, the end faces of the left side and the right side of the placing frame are uniformly and integrally formed with convex strips, and the convex strips are inserted into the slots.

Preferably, the end cover is internally sleeved with a sealing ring, the sealing ring can be covered on the end face of the top end of the storage box, a plurality of abutting blocks are tightly bonded on the bottom face of the end cover, the positions of the abutting blocks correspond to those of the storage test tubes, and when the end cover is folded on the storage box, the abutting blocks abut against the top end of the tube opening sealing device.

Preferably, the top of the placing frame is provided with a plurality of storing hole sites, and the storing test tubes are inserted into the storing hole sites and are lapped at the bottom of the placing frame.

Preferably, the top end of the convex strip is connected with a vertical rod in a threaded manner, and the top end of the vertical rod is tightly welded with a lifting plate.

Preferably, the top end of the sleeve column is provided with a cavity, the bottom end of the sleeve column is provided with a sleeve hole communicated with the cavity, the diameter of the sleeve hole is smaller than that of the cavity, the pipe orifice of the storage test tube penetrates through the sleeve hole and is inserted into the cavity, the inner wall of the sleeve hole is provided with a ring groove, a spacing ring is embedded in the ring groove, and the inner side surface of the spacing ring is tightly attached to the outer surface of the storage test tube.

Preferably, the peripheral surface of the top of the sleeve column is in a threaded shape, a convex edge is integrally formed at the top end of the sleeve column, the outer diameter of the convex edge is smaller than that of the sleeve column, the sealing rubber sleeve is sleeved at the top of the convex edge and wraps the convex edge, and when the sealing cover is in a screwed state at the top end of the sleeve column, the sealing cover tightly extrudes the sealing rubber sleeve at the top end of the convex edge.

Compared with the prior art, the invention has the beneficial effects that:

according to the invention, the placing frame provided with the storage test tube is placed into the storage box filled with the sterilizing solution, so that the whole storage test tube can be soaked in the sterilizing solution after the end cover is arranged, tumor cells stored in the storage test tube are isolated from the external bacteria environment, and the stored tumor cells are prevented from being polluted by external bacteria.

Drawings

FIG. 1 is a schematic view of the overall structure of the present invention;

FIG. 2 is an exploded view of the overall structure of the present invention;

FIG. 3 is a schematic structural view of a storage case according to the present invention;

FIG. 4 is an exploded view of the bottom structure of the end cap of the present invention;

FIG. 5 is a schematic structural view of the rack of the present invention;

FIG. 6 is an exploded view of the orifice sealing device of the present invention;

FIG. 7 is a cross-sectional view of a collet of the present invention;

fig. 8 is a partial cross-sectional view of the nozzle sealing device of the present invention wrapping a storage tube.

The meaning of the individual reference symbols in the figures is:

1. a storage box; 11. a storage chamber; 111. a slot; 112. a guide groove; 12. a lug; 121. a threaded hole; 2. an end cap; 21. a convex plate; 22. twisting a handle; 221. a threaded post; 23. a seal ring; 24. a resisting block; 3. placing a rack; 31. storing hole sites; 32. a convex strip; 321. erecting a rod; 322. lifting the plate; 4. storing the test tubes; 5. a pipe orifice sealing device; 51. sleeving a column; 511. a cavity; 512. trepanning; 5121. a ring groove; 513. a convex edge; 52. sealing the rubber sleeve; 53. a sealing cover; 54. and a space ring.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

In the description of the present invention, it is to be understood that the terms "left", "right", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on those shown in the drawings, and are used only for convenience in describing the present invention and for simplicity in description, and do not indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and thus, are not to be construed as limiting the present invention.

Referring to fig. 1 to 8, the present invention provides a technical solution:

the utility model provides a can completely cut off external bacterium's tumour cell temporary storage device, including storing box 1, it stores chamber 11 to have seted up in the storage box 1, it has bactericidal solution to pour into in the storage chamber 11, the top of storing box 1 is equipped with end cover 2, it has lug 12 to store homogeneous body shaping on the left and right sides terminal surface at box 1 top, set up threaded hole 121 on the lug 12, homogeneous body shaping has flange 21 on the left and right sides terminal surface of end cover 2, be equipped with on the flange 21 and twist 22, it is equipped with screw thread post 221 to twist the bottom of 22, screw thread post 221's bottom run through flange 21 and with screw hole 121 threaded connection. When the end cap 2 is folded to the top end of the storage box 1 and the screw handle 22 is screwed, the convex plate 21 is fixed with the lug 12 under the action of the threaded column 221, so that the end cap 2 is fixed at the top end of the storage box 1, and the sterilizing solution injected into the storage box 1 is prevented from leaking out of the storage box 1.

In the invention, the sealing ring 23 is sleeved in the end cover 2, and the sealing ring 23 can be covered on the end surface of the top end of the storage box 1, so that when the end cover 2 is fixed to the top end of the storage box 1, the sealing ring 23 is extruded by the top ends of the end cover 2 and the storage box 1, the storage box 1 and the end cover 2 are sealed by the sealing ring 23, and the leakage of the sterilizing solution is further prevented.

Specifically, place rack 3 in the storage chamber 11, a plurality of hole sites 31 of depositing have been seted up at rack 3's top, have placed on rack 3 and have stored test tube 4, store test tube 4 and insert and deposit in the hole site 31 and the overlap joint in rack 3's bottom, can deposit a plurality of storage test tubes 4 in the messenger rack 3 simultaneously. All seted up slot 111 on storing the left and right sides inner wall in chamber 11, homogeneous body shaping has sand grip 32 on rack 3's the left and right sides terminal surface, sand grip 32 inserts in slot 111, through align sand grip 32 on rack 3 with slot 111 after down pressing down rack 3 can make rack 3 be restricted between two slot 111 under the effect of sand grip 32, reach in storing box 1 to the spacing purpose of rack 3, prevent that rack 3 from taking place the slope in storing box 1. The top notch of the slot 111 is provided with a guide slot 112, and the notch size of the guide slot 112 is larger than the opening size of the slot 111, so that the convex strip 32 can be conveniently inserted into the slot 111 under the guidance of the guide slot 112, and the storage box 1 can be conveniently placed in the placing frame 3.

Further, the top end threaded connection of sand grip 32 has pole setting 321, and the top of pole setting 321 closely welds and has carried board 322, when rack 3 was located and stores box 1, can make and carry board 322 upwards pull rack 3 through pole setting 321 through upwards mentioning and carrying board 322, makes rack 3 deviate from in can following storing box 1, reaches the purpose of taking out rack 3.

In addition, the mouth of pipe department of storing test tube 4 is provided with mouth of pipe sealing device 5, and mouth of pipe sealing device 5 includes cover post 51, sealed gum cover 52 and sealed lid 53, and the orificial outside at storing test tube 4 is established to cover post 51 cover, and sealed gum cover 52 covers the top of establishing at cover post 51, and sealed lid 53 threaded connection is on the top of cover post 51, and sealed lid 53 covers the outside of establishing on storing test tube 4 top. The top of sleeve post 51 has seted up cavity 511, the trepanning 512 that is linked together with cavity 511 is seted up to the bottom of sleeve post 51, the diameter of trepanning 512 is less than the diameter of cavity 511, the mouth of pipe of storage test tube 4 passes from trepanning 512 and inserts inside the cavity 511, annular 5121 has been seted up on the inner wall of trepanning 512, the embedded spacer ring 54 that is equipped with of annular 5121, the inboard surface of spacer ring 54 and the surface of storage test tube 4 closely laminate, make the space between storage test tube 4 and the sleeve post 51 sealed by spacer ring 54, prevent that the antiseptic solution in the storage chamber 11 from stretching into in the sleeve post 51.

It should be noted that the peripheral surface of the top of the socket 51 is threaded, so that the sealing cover 53 can be screwed on the top of the socket 51, the top of the socket 51 is integrally formed with a convex edge 513, the outer diameter of the convex edge 513 is smaller than the outer diameter of the socket 51, the sealing rubber sleeve 52 is sleeved on the top of the convex edge 513 and wraps the convex edge 513 inside, when the sealing cover 53 is screwed on the top of the socket 51, the sealing cover 53 presses the sealing rubber sleeve 52 tightly against the top of the convex edge 513, so that the top of the convex edge 513 is sealed by the sealing rubber sleeve 52, further preventing the sterilization solution in the storage chamber 11 from permeating into the socket 51, under the sealing protection of the nozzle sealing device 5, the nozzle of the storage tube 4 is wrapped by the nozzle sealing device 5, so that the nozzle of the storage tube 4 is isolated from the sterilization solution in the storage chamber 11 by the nozzle sealing device 5, and preventing the nozzle of the storage tube 4 from being stained with the sterilization solution, thereby preventing the antiseptic solution from infiltrating into the storage tube 4 and damaging the stored tumor cells.

It is worth to say that, a plurality of resisting blocks 24 are closely bonded on the bottom surface of the end cover 2, the resisting blocks 24 are made of flexible rubber materials, the positions of the resisting blocks 24 correspond to the positions of the storage test tubes 4, when the end cover 2 is folded on the storage box 1, the resisting blocks 24 are abutted against the top end of the tube opening sealing device 5, so that the top ends of the storage test tubes 4 are abutted by the resisting blocks 24, the storage test tubes 4 can be completely soaked in the sterilization solution, the phenomenon that the storage test tubes 4 float in the storage cavity 11 due to air left in the storage test tubes 4 is prevented, the storage test tubes 4 are isolated from the external bacteria environment, and the storage test tubes 4 are prevented from being adhered by external bacteria.

It should be added that the sterilizing solution filled in the storage cavity 11 can be 75% medical alcohol, the molecules of the alcohol have great permeability, and the sterilizing solution can penetrate through the membrane on the surface of the bacteria and enter the interior of the bacteria, so that the protein forming the life base of the bacteria is solidified, the bacteria are killed, and the storage test tube 4 soaked in the storage cavity 11 can be isolated from the outside bacteria.

When the temporary storage device for tumor cells capable of isolating external bacteria is used, firstly, a storage test tube 4 filled with tumor cells penetrates through a sleeve column 51, then a sealing cover 53 is screwed at the top end of the sleeve column 51, a tube mouth sealing device 5 is wrapped at the tube mouth of the storage test tube 4, the tube mouth of the storage test tube 4 is isolated, then the storage test tube 4 is inserted into a storage hole 31 in a placing frame 3, then a raised strip 32 on the placing frame 3 is aligned with a slot 111, then the placing frame 3 is pressed downwards, the placing frame 3 is limited between the two slots 111 under the action of the raised strip 32, at the moment, the placing frame 3 is sleeved with the whole storage test tube 4 in a storage cavity 11, finally, a sterilizing solution is injected into the storage cavity 11, the sterilizing solution is soaked in the whole storage test tube 4, an end cover 2 is folded at the top end of the storage box 1, and the end cover 2 is fixed at the top end of the storage box 1 through screwing a handle 22, make and store test tube 4 and can seal in the inside of storing box 1, make and store test tube 4 and keep apart with the external world through bactericidal solution to prevent that external bacterium from causing the pollution to the tumor cell who stores in the test tube 4.

The foregoing shows and describes the general principles, essential features, and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, and the preferred embodiments of the present invention are described in the above embodiments and the description, and are not intended to limit the present invention. The scope of the invention is defined by the appended claims and equivalents thereof.

Claims

1. The utility model provides a can isolate external bacterium's tumor cell temporary storage device, includes storage box (1), its characterized in that: store and seted up storage chamber (11) in box (1), it has the bactericidal solution to inject in storage chamber (11), the top of storing box (1) is equipped with end cover (2), rack (3) have been placed in storage chamber (11), placed on rack (3) and stored test tube (4), the mouth of pipe department of storing test tube (4) is provided with mouth of pipe sealing device (5), mouth of pipe sealing device (5) are including socle (51), sealed gum cover (52) and sealed lid (53), socle (51) cover is established store test tube (4) orificial outside, sealed gum cover (52) are covered and are established on the top of socle (51), sealed lid (53) threaded connection is on the top of socle (51), and sealed lid (53) cover is established in the outside on storage test tube (4) top.

2. The temporary tumor cell buffer device capable of isolating external bacteria as claimed in claim 1, wherein: it has lug (12) to form a whole on the left and right sides terminal surface at storage box (1) top, set up threaded hole (121) on lug (12), the homogeneous shaping has flange (21) on the left and right sides terminal surface of end cover (2), be equipped with on flange (21) and twist (22), it inlays and is equipped with screw thread post (221) to twist the bottom of (22), screw thread post (221) run through flange (21) and with screw hole (121) threaded connection.

3. The temporary tumor cell buffer device capable of isolating external bacteria as claimed in claim 1, wherein: all seted up slot (111) on the left and right sides inner wall of storage chamber (11), guide way (112) have been seted up to the top notch department of slot (111), homogeneous body shaping has sand grip (32) on the left and right sides terminal surface of rack (3), sand grip (32) insert in slot (111).

4. The temporary tumor cell buffer device capable of isolating external bacteria as claimed in claim 1, wherein: the sealing device is characterized in that a sealing ring (23) is sleeved in the end cover (2), the sealing ring (23) can be covered on the end face of the top end of the storage box (1), a plurality of abutting blocks (24) are tightly adhered to the bottom face of the end cover (2), the positions of the abutting blocks (24) correspond to the positions of the storage test tubes (4), and when the end cover (2) is folded on the storage box (1), the abutting blocks (24) abut against the top end of the tube opening sealing device (5).

5. The temporary tumor cell buffer device capable of isolating external bacteria as claimed in claim 1, wherein: a plurality of storage hole sites (31) are arranged at the top of the placing frame (3), and the storage test tubes (4) are inserted into the storage hole sites (31) and are lapped at the bottom of the placing frame (3).

6. The temporary tumor cell buffer device for isolating external bacteria as claimed in claim 3, wherein: the top end threaded connection of sand grip (32) has pole setting (321), the top of pole setting (321) is closely welded and is had lifting plate (322).

7. The temporary tumor cell buffer device capable of isolating external bacteria as claimed in claim 1, wherein: cavity (511) have been seted up on the top of cover post (51), the bottom of cover post (51) seted up with trepanning (512) that cavity (511) are linked together, the diameter of trepanning (512) is less than the diameter of cavity (511), the mouth of pipe of storing test tube (4) is followed pass in trepanning (512) and insert cavity (511) inside, annular (5121) have been seted up on the inner wall of trepanning (512), the embedded spacer ring (54) that is equipped with of annular (5121), the inboard surface of spacer ring (54) with the surface of storing test tube (4) closely laminates.

8. The temporary tumor cell buffer device for isolating external bacteria as claimed in claim 7, wherein: the peripheral surface at cover post (51) top is the screw thread form, the top integrated into one piece of cover post (51) has protruding edge (513), the external diameter of protruding edge (513) is less than the external diameter of cover post (51), sealed gum cover (52) cover is established protruding top along (513) and will protruding edge (513) parcel is including, works as sealed lid (53) are in when the top of cover post (51) is the state of screwing, sealed lid (53) will sealed gum cover (52) are crowded tightly and are in the top of protruding edge (513).