CN113985011A - Device for testing biotoxicity of volatile organic contaminated soil and using method thereof - Google Patents
Device for testing biotoxicity of volatile organic contaminated soil and using method thereof Download PDFInfo
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- CN113985011A CN113985011A CN202111456196.1A CN202111456196A CN113985011A CN 113985011 A CN113985011 A CN 113985011A CN 202111456196 A CN202111456196 A CN 202111456196A CN 113985011 A CN113985011 A CN 113985011A
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- bottle
- sponge
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- gas washing
- glass partition
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- 238000012360 testing method Methods 0.000 title claims abstract description 44
- 239000002689 soil Substances 0.000 title claims abstract description 43
- 238000000034 method Methods 0.000 title abstract description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 7
- 238000005192 partition Methods 0.000 claims description 41
- 239000011521 glass Substances 0.000 claims description 34
- 238000005406 washing Methods 0.000 claims description 34
- 230000002572 peristaltic effect Effects 0.000 claims description 14
- 239000003292 glue Substances 0.000 claims description 10
- 230000009191 jumping Effects 0.000 claims description 10
- 239000000126 substance Substances 0.000 claims description 8
- 239000007788 liquid Substances 0.000 claims description 6
- 239000008367 deionised water Substances 0.000 claims description 3
- 229910021641 deionized water Inorganic materials 0.000 claims description 3
- 241001411320 Eriogonum inflatum Species 0.000 claims description 2
- 239000003344 environmental pollutant Substances 0.000 abstract description 13
- 231100000719 pollutant Toxicity 0.000 abstract description 13
- 241001427559 Collembola Species 0.000 abstract description 3
- 230000035945 sensitivity Effects 0.000 abstract description 3
- 239000007789 gas Substances 0.000 description 26
- 239000000243 solution Substances 0.000 description 12
- 238000001514 detection method Methods 0.000 description 3
- 238000005201 scrubbing Methods 0.000 description 3
- 231100000331 toxic Toxicity 0.000 description 3
- 230000002588 toxic effect Effects 0.000 description 3
- 241000238631 Hexapoda Species 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 238000003745 diagnosis Methods 0.000 description 2
- 229910001385 heavy metal Inorganic materials 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 239000012085 test solution Substances 0.000 description 2
- 231100000041 toxicology testing Toxicity 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 210000001015 abdomen Anatomy 0.000 description 1
- 230000003187 abdominal effect Effects 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 239000006059 cover glass Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000002957 persistent organic pollutant Substances 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 239000003802 soil pollutant Substances 0.000 description 1
- 238000003900 soil pollution Methods 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 239000012855 volatile organic compound Substances 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/24—Earth materials
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Food Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Geology (AREA)
- General Life Sciences & Earth Sciences (AREA)
- Environmental & Geological Engineering (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Remote Sensing (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
- Processing Of Solid Wastes (AREA)
Abstract
The invention provides a biotoxicity testing device for volatile organic contaminated soil and a using method thereof. The device maintains the wetness of the sponge through the solution containing volatile pollutants siphoned into the sponge by the capillary, ensures the humid environment condition required by the Baiyitiao and simultaneously tests the behavior of the springtail avoidance. The device can simultaneously test the influence of volatile pollutants in air and water solution on the white symbol jump, and has high sensitivity.
Description
Technical Field
The invention relates to a toxicity testing device for volatile organic contaminated soil organisms and a using method thereof, belonging to the field of comprehensive toxicity testing methods for soil organisms aiming at soil habitat functions.
Background
Volatile organic compounds, as a class of specific soil pollutants, are classified as pollutants with high potential risks in the environment and to be preferentially controlled due to the complexity and harmfulness of the components. The pollution diagnosis of the site soil can provide important basis for site management decision. At the present stage, the soil pollution diagnosis in China is mainly based on pollutant concentration analysis, and has the defects of high detection cost, direct influence on detection results by preset detection indexes, incapability of reflecting comprehensive toxicity of complex multi-component pollutants and the like.
The comprehensive toxic effect of the polluted site is directly reflected by using sensitive organisms, the comprehensive toxic effect of the soil sample on organism individuals and populations can be represented, and the method has the characteristics of simplicity, convenience, economy, capability of screening polluted soil with various batches, varieties and different concentrations and the like. However, the existing testing method is only suitable for non-volatile organic pollution and heavy metal pollution sites, and is not suitable for volatile organic pollution soil.
The Baiyitiao is a representative of sensitive organisms in a soil ecosystem and is widely applied to environmental hazard assessment of pesticides, polluted soil and the like. The horn base of the Baiyitiao has a horn retrocessor which can be used for monitoring chemical substances in the air, and the ventral surface of the first section of the abdomen is provided with a pair of abdominal tubes which can communicate liquid with the external environment. Therefore, white-symbol jumps are a good target for monitoring volatile organic pollutants. A plurality of test methods are formulated by ISO based on animals of the order Largetoria such as Baiyitiao, but the methods are suitable for non-volatile organic pollution and heavy metal pollution sites and are not suitable for volatile organic pollution soil.
Disclosure of Invention
The invention aims to solve the defects in the prior art and provides a biotoxicity testing device suitable for volatile organic contaminated soil.
In order to achieve the purpose, the device is realized by the following technical scheme:
the device consists of a soil sample bottle, a gas washing and testing device bottle and a peristaltic pump.
The soil sample bottle is a cylindrical glass bottle with a plug and an upper opening and a lower opening, a guide pipe hole is formed in the plug, and a sieve plate is arranged in the bottle.
The gas washing and testing device bottle consists of a bottle plug and a bottle body, wherein the bottle plug is provided with a guide pipe hole, a glass partition is arranged in the upper cover, the bottle body is provided with a guide pipe socket, and the glass partition and a capillary pipe are arranged in the bottle body.
The upper part of the gas washing and testing device bottle is connected with the bottom of the soil sample bottle through a conduit, a peristaltic pump is arranged on the conduit, and the conduit connected with the upper part of the soil sample bottle penetrates through a conduit socket of the body of the gas washing and testing device bottle and is inserted into the bottom of the bottle.
Specifically, soil sample bottle top opening is greater than the below opening, the sieve is located soil sample bottle and is close to the bottom position.
Specifically, the glass partition inside the bottle body of the gas washing and testing device is positioned on the upper portion of the bottle body and parallel to the bottom of the bottle, the glass partition is in a shape of a U-arc, an arc edge is attached to the bottle body, an air passage partition perpendicular to the glass partition is arranged on a straight line edge, the height of the air passage partition is 20-30 mm, small holes are evenly distributed in the glass partition, the glass partition is divided into two areas by a transverse partition in an equal mode, a sponge is placed in one area, a capillary tube is inserted into the sponge and penetrates through the small hole in the glass partition to be perpendicular to the bottom surface downwards, and oily glue free of volatile substances is coated in the other area.
Specifically, the glass partition in the upper cover of the gas washing and testing device is parallel to the bottom of the bottle, and the bottom surface of the glass partition is coated with oily glue without volatile substances.
Specifically, the sponge is black and 5-10mm thick.
The invention also aims to provide a using method of the device, which is to fill soil to be detected on a sieve plate of a soil sample bottle; filling deionized water in the gas washing and testing device bottle as a gas washing solution, wherein the liquid level is lower than the glass partition in the bottle body; tightly covering all bottle stoppers, starting a peristaltic pump to pump gas above a gas washing and testing device bottle into the bottom of a soil sample bottle, then entering gas washing and testing device bottle gas washing solution from the top, pausing the peristaltic pump after sponge on a glass partition is wetted, opening an upper cover of the gas washing and testing device bottle, adding white-symbol jumping larvae on the sponge, covering the upper cover, starting the peristaltic pump, timing and recording the number of white-symbol jumping out of the sponge and the number of residual jumping insects on the sponge.
The device for testing the biotoxicity of the volatile organic contaminated soil has the following advantages: the solution containing volatile pollutants siphoned into the sponge through the capillary tube maintains the humidity of the sponge to ensure the humid environment condition required by the white jump on the one hand, and is also a test solution for testing the jumping pest avoidance behavior on the other hand. The holes in zone 9A allow excess solution siphoned to the sponge to leak back into the gas-liquid. The influence of volatile pollutants in air and water solution on the white symbol jump can be tested simultaneously, and the sensitivity is high. And the oily glue without volatile substances is used for coating part of the area, so that the phenomenon that part of white symbols jump to have avoidance behavior can be avoided, and after the white symbols jump out of the sponge, the avoidance behavior jumps back to the sponge area due to the influence of volatile pollutants in the air.
Drawings
FIG. 1 is a schematic view of the overall structure of the present invention;
FIG. 2 is a schematic view of the upper cap of the bottle of the gas scrubbing and testing apparatus;
FIG. 3 is a bottom view of the upper cap of the bottle of the apparatus for scrubbing and testing;
FIG. 4 is a schematic view of a bottle body of the gas washing and testing apparatus;
FIG. 5 is a top view of a bottle body of the gas scrubbing and testing apparatus;
in the figure: 1-soil sample bottle, 2-soil sample containing volatile pollutants, 3-sieve plate, 4-silica gel bottle plug, 5-air guide tube, 6-peristaltic pump, 7-upper cover glass partition, 8-sponge, 9-body glass partition, 10-capillary, 11-gas washing solution, 12-gas washing and testing device bottle, 9A-body glass partition sponge area, 9B-body glass partition glue area, 9C-sponge area and glue area cross partition, and 9D-air channel partition plate of body glass partition.
Detailed Description
The following description will explain embodiments of the present invention with reference to the accompanying drawings.
A device for testing the biotoxicity of volatile organic contaminated soil is shown in figure 1, and when the device is used, a certain amount (such as 500g) of soil to be tested is filled in a soil sample bottle 1.
In the air washer and tester bottle 12, an oily glue containing no volatile substance was applied to the lower side of the upper lid glass partition 7. Sponge is placed on the area A of the glass partition 9 of the bottle body, oily glue without volatile substances is smeared on the area B, meanwhile, capillaries are placed on the bottom surface of the sponge, deionized water is filled at the bottom of the bottle to serve as a gas washing solution, and the liquid level is lower than the glass partition 9 of the bottle body.
And starting the peristaltic pump 6 to enable the air flow to enter the bottom of the soil sample bottle 1 from the bottom, to enter the gas washing and testing device bottle 12 after the air flow goes out of the soil sample bottle 1 from the top, to go out of the gas washing and testing device bottle 12 through a channel above the sponge 8 after passing through the gas washing liquid, and to return to the soil sample bottle 1 through the peristaltic pump to enter the next cycle.
After running for 2h, the sponge placed on the area A of the glass partition 9 of the bottle body is observed to be wetted through the air washing and testing device bottle 12. The peristaltic pump 6 is stopped, the upper cover of the bottle 12 of the gas washing and testing device is opened, N (30 are recommended) white-symbol jumping larvae with the age of 9-12 days are gently added on the sponge, the upper cover of the bottle 12 of the gas washing and testing device is covered, the peristaltic pump is started, and the timing is started. Observing for 15 minutes, and recording the number N of white symbols jumping out of the sponge1。
Continuously running for 48h, and recording the number N of the residual springtails on the sponge2。
The avoidance rate calculation of springtails is disclosed as follows:
the volatile gas circulates in the closed system, so that the volatile pollutants can be gradually distributed and balanced in the water, soil and gas in the system. The solution containing volatile pollutants siphoned into the sponge through the capillary maintains the wetness of the sponge and guarantees the humid environment condition required by the white-fleshed jump on the one hand, and the solution is also a test solution for testing the flea-fleshed insect avoidance behavior on the other hand. When volatile pollutants in the solution and the airflow have toxic effect on the Baiyitiao, the Baiyitiao generates avoidance behavior and bounces to the B area on the glass partition 9 of the bottle body. Since this area has been smeared with oily glue, the white jump jumping to this area will not jump again. The device can be used for simultaneously testing the influence of volatile pollutants in air and water solution on the white symbol jump, and has high sensitivity.
The above is merely a basic embodiment of the present invention according to the technical solution, and any similar modifications proposed according to the present invention should be considered as the protection scope of the present invention.
Claims (6)
1. The device for testing the biotoxicity of the volatile organic contaminated soil is characterized by comprising a soil sample bottle, a gas washing and testing device bottle and a peristaltic pump;
the soil sample bottle is a cylindrical glass bottle with a plug and an upper opening and a lower opening, a guide pipe hole is formed in the plug, and a sieve plate is arranged in the bottle;
the gas washing and testing device bottle consists of a bottle plug and a bottle body, wherein the bottle plug is provided with a guide pipe hole, a glass partition is arranged in the upper cover, the bottle body is provided with a guide pipe socket, and the glass partition and a capillary pipe are arranged in the bottle body;
the upper part of the gas washing and testing device bottle is connected with the bottom of the soil sample bottle through a conduit, the conduit is connected with a peristaltic pump, and the conduit connected with the upper part of the soil sample bottle penetrates through a conduit socket of the body of the gas washing and testing device bottle and is inserted into the bottom of the bottle.
2. The apparatus of claim 1, wherein the soil sample bottle has a larger upper opening than a lower opening, and wherein the screen is positioned within the soil sample bottle near the bottom.
3. The device of claim 1, wherein the glass partition inside the bottle body of the washing and testing device is positioned on the upper portion of the bottle body and parallel to the bottom of the bottle, the glass partition is in a shape of a U-arc segment, an arc edge is attached to the bottle body, an air passage partition perpendicular to the glass partition is arranged on a straight line edge, the height of the air passage partition is 20mm-30mm, small holes are uniformly distributed in the glass partition, the glass partition is divided into two areas by a transverse partition, a sponge is placed in one area, a capillary tube is inserted into the sponge, the capillary tube penetrates through the small holes in the glass partition and is perpendicular to the bottom surface downwards, and the other area is coated with oily glue without volatile substances.
4. The device according to claim 1, characterized in that the glass partition in the upper lid of the bottle of the washing and testing device is semicircular and parallel to the bottom of the bottle, and the lower side of the glass partition is coated with oily glue without volatile substances.
5. A device according to claim 3, wherein the sponge is black and 5-10mm thick.
6. The use of the device according to claims 1-5, characterized in that the soil to be tested is filled on the sieve plate of the soil sample bottle; filling deionized water in the gas washing and testing device bottle as a gas washing solution, wherein the liquid level is lower than the glass partition in the bottle body; tightly covering all bottle stoppers, starting a peristaltic pump to pump gas above a gas washing and testing device bottle into the bottom of a soil sample bottle, then entering gas washing and testing device bottle gas washing solution from the top, pausing the peristaltic pump after sponge is wetted, opening an upper cover of the gas washing and testing device bottle, adding Chinese character ' bai ' jumping larvae on the sponge, covering the upper cover, starting the peristaltic pump, timing and recording the number of Chinese character ' bai jumping out of the sponge and the number of residual jumping worms on the sponge.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111456196.1A CN113985011A (en) | 2021-12-02 | 2021-12-02 | Device for testing biotoxicity of volatile organic contaminated soil and using method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111456196.1A CN113985011A (en) | 2021-12-02 | 2021-12-02 | Device for testing biotoxicity of volatile organic contaminated soil and using method thereof |
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| Publication Number | Publication Date |
|---|---|
| CN113985011A true CN113985011A (en) | 2022-01-28 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111456196.1A Pending CN113985011A (en) | 2021-12-02 | 2021-12-02 | Device for testing biotoxicity of volatile organic contaminated soil and using method thereof |
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| CN (1) | CN113985011A (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102495198A (en) * | 2011-12-06 | 2012-06-13 | 中国科学院生态环境研究中心 | Method for identifying soil pollution under assistance of springtail reproduction |
| CN105717275A (en) * | 2016-01-28 | 2016-06-29 | 东南大学 | One-dimensional testing device for simulating transport of volatile organic pollutants in soil |
| US20160266084A1 (en) * | 2015-03-09 | 2016-09-15 | Scott Russell Burge | Unified Sampling and Analytical System for Monitoring Volatile Chemicals in Ground Water, Soil-Gas and Indoor Air Quality with Sample Collection For Laboratory Analysis |
| CN206192994U (en) * | 2016-11-29 | 2017-05-24 | 中国科学院南京土壤研究所 | Test device is avoided to soil springtail |
| CN208780701U (en) * | 2018-06-27 | 2019-04-23 | 广州欣源生物科技有限公司 | Geobiont toxicity test device |
| CN211603178U (en) * | 2019-12-31 | 2020-09-29 | 中国地质科学院水文地质环境地质研究所 | Detection device and detection system for volatile organic compounds in soil |
| CN216411292U (en) * | 2021-12-02 | 2022-04-29 | 生态环境部南京环境科学研究所 | Device for testing biotoxicity of volatile organic contaminated soil |
-
2021
- 2021-12-02 CN CN202111456196.1A patent/CN113985011A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102495198A (en) * | 2011-12-06 | 2012-06-13 | 中国科学院生态环境研究中心 | Method for identifying soil pollution under assistance of springtail reproduction |
| US20160266084A1 (en) * | 2015-03-09 | 2016-09-15 | Scott Russell Burge | Unified Sampling and Analytical System for Monitoring Volatile Chemicals in Ground Water, Soil-Gas and Indoor Air Quality with Sample Collection For Laboratory Analysis |
| CN105717275A (en) * | 2016-01-28 | 2016-06-29 | 东南大学 | One-dimensional testing device for simulating transport of volatile organic pollutants in soil |
| CN206192994U (en) * | 2016-11-29 | 2017-05-24 | 中国科学院南京土壤研究所 | Test device is avoided to soil springtail |
| CN208780701U (en) * | 2018-06-27 | 2019-04-23 | 广州欣源生物科技有限公司 | Geobiont toxicity test device |
| CN211603178U (en) * | 2019-12-31 | 2020-09-29 | 中国地质科学院水文地质环境地质研究所 | Detection device and detection system for volatile organic compounds in soil |
| CN216411292U (en) * | 2021-12-02 | 2022-04-29 | 生态环境部南京环境科学研究所 | Device for testing biotoxicity of volatile organic contaminated soil |
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