CN113984584A - Portable hemorheological instrument test box for trace detection and hemorheological detection method - Google Patents
Portable hemorheological instrument test box for trace detection and hemorheological detection method Download PDFInfo
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- CN113984584A CN113984584A CN202111279631.8A CN202111279631A CN113984584A CN 113984584 A CN113984584 A CN 113984584A CN 202111279631 A CN202111279631 A CN 202111279631A CN 113984584 A CN113984584 A CN 113984584A
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- 238000012360 testing method Methods 0.000 title claims abstract description 92
- 238000001514 detection method Methods 0.000 title claims abstract description 86
- 238000002347 injection Methods 0.000 claims abstract description 18
- 239000007924 injection Substances 0.000 claims abstract description 18
- 230000004087 circulation Effects 0.000 claims abstract description 8
- 238000010438 heat treatment Methods 0.000 claims abstract description 8
- 230000005540 biological transmission Effects 0.000 claims description 25
- 210000004369 blood Anatomy 0.000 claims description 23
- 239000008280 blood Substances 0.000 claims description 23
- 239000012780 transparent material Substances 0.000 claims description 3
- 238000000518 rheometry Methods 0.000 claims description 2
- 238000005259 measurement Methods 0.000 claims 1
- 210000004204 blood vessel Anatomy 0.000 description 4
- 230000003044 adaptive effect Effects 0.000 description 2
- 230000017531 blood circulation Effects 0.000 description 2
- 239000012503 blood component Substances 0.000 description 2
- 238000001962 electrophoresis Methods 0.000 description 2
- 210000003743 erythrocyte Anatomy 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 206010008132 Cerebral thrombosis Diseases 0.000 description 1
- 102000008946 Fibrinogen Human genes 0.000 description 1
- 108010049003 Fibrinogen Proteins 0.000 description 1
- 201000001429 Intracranial Thrombosis Diseases 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000023555 blood coagulation Effects 0.000 description 1
- 230000036772 blood pressure Effects 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 229940012952 fibrinogen Drugs 0.000 description 1
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- 238000004062 sedimentation Methods 0.000 description 1
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N11/00—Investigating flow properties of materials, e.g. viscosity, plasticity; Analysing materials by determining flow properties
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
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Abstract
The invention relates to the technical field of hemorheology detection, in particular to a portable hemorheology instrument test box for trace detection and a hemorheology detection method, wherein the test box comprises a test box body and a detection piece, a control detection module, a micropump, a first electromagnetic valve, a second electromagnetic valve and a puncture needle base are fixedly arranged on the bottom surface in the test box body, a puncture needle is vertically arranged on the puncture needle base, the needle tail of the puncture needle is connected with a three-way joint, a detection pipeline is connected with the control detection module, a pressure supply pipeline is connected with the micropump, the second electromagnetic valve controls the circulation and the blockage of the pressure supply pipeline, the detection piece comprises a sample injection head, a capillary pipeline and an air bag cavity, an air bag is arranged in the air bag cavity, the puncture needle penetrates into a rubber plug of the air bag, the first electromagnetic valve controls the circulation and the blockage of the capillary pipeline, and a capillary heating groove is arranged on the upper surface of the test box body; the invention can effectively solve the problems of large sample consumption and complex operation, realizes small equipment volume and convenient carrying, and is suitable for clinics and outgoing treatment.
Description
Technical Field
The invention relates to the technical field of hemorheology detection, in particular to a portable hemorheology instrument test box for trace detection and a hemorheology detection method.
Background
Hemorheology includes more than 10 indexes of whole blood specific viscosity, whole blood reduced viscosity, plasma viscosity, erythrocyte electrophoresis time, platelet electrophoresis time, fibrinogen determination, blood sedimentation, erythrocyte deformability and the like. Mainly reflects the changes of blood fluidity, blood coagulation and blood viscosity caused by the changes of blood components. Normally, blood flows in a blood vessel by an external force (blood pressure), and changes according to the properties of the blood vessel (such as the condition of the blood vessel wall and the shape of the blood vessel) and the change of blood components (viscosity), thereby maintaining normal blood circulation. When the viscosity of blood becomes higher, the fluidity of blood becomes worse, and cerebral thrombosis is most likely to occur. Conversely, the viscosity is lower and the fluidity is better.
The current method of analysis is to draw venous blood into a test tube and then draw a blood sample from the tube for testing. The method has the advantages of more blood samples extracted, complex operation, increased pain and risk of patients, requirement of professional technical skills and suitability for large hospitals and detection institutions.
Disclosure of Invention
Aiming at the defects of the technology, the invention aims to solve the problems that: how to provide a portable hemorheology instrument test box for trace detection and an adaptive detection method to solve the problems of large sample consumption, complex operation and the like of the conventional hemorheology detection, realize small equipment volume and convenient carrying, and are suitable for small hospitals, clinics and outgoing clinics.
In order to solve the problems, the invention adopts the following technical scheme:
a portable hemorheology instrument test box for trace detection comprises a test box body and a detection piece movably embedded on the upper surface of the test box body, wherein a control detection module, a micropump, a first electromagnetic valve, a second electromagnetic valve and a puncture needle base are fixedly arranged on the bottom surface in the test box body; a puncture needle is vertically arranged on the puncture needle base, the needle tail of the puncture needle is fixedly arranged in the puncture needle base, the needle tail of the puncture needle is connected with a first joint of a three-way joint, a second joint of the three-way joint is connected with the control detection module through a detection pipeline, and a third joint of the three-way joint is connected with the micropump through a pressure supply pipeline; the second solenoid valve controls the flow and the blockage of the pressure supply pipeline;
the detection part comprises a sample injection head, a capillary channel and an air bag cavity, wherein an air bag is arranged in the air bag cavity, and a rubber plug is arranged at the bottom of the air bag; the sample injection head is provided with a sample injection port, the sample injection port is communicated with one end of the capillary pipeline, and the other end of the capillary pipeline is communicated with the air bag; the puncture needle pierces the rubber plug of the air bag, so that the detection piece is movably embedded on the upper surface of the test box body;
the first solenoid valve controls the circulation and the blockage of the capillary pipeline;
the test box comprises a test box body and is characterized in that a capillary heating groove is formed in the upper surface of the test box body, corresponds to the capillary pipeline and is arranged along the direction of the capillary pipeline.
Further, a printer is fixedly arranged on the bottom surface inside the test box body and is electrically connected with the control detection module; the display opening of printer is left on the test box body upper surface.
Furthermore, a storage battery is fixedly arranged on the bottom surface in the test box body and is electrically connected with the control detection module; the storage battery is connected with a charging connector, a charging connector opening is formed in the side edge of the test box body, and the charging connector is arranged at the charging connector opening.
Further, the electric joint opening (3-1) and the data transmission opening (3-2) are arranged at the left part of the bottom edge of the side edge of the test box body (1).
Furthermore, the control detection module is provided with a data transmission joint, a data transmission opening is formed in the side edge of the test box body, and the data transmission joint is arranged at the data transmission opening; and the control detection module performs data transmission with external equipment through the data transmission joint or wireless transmission.
Further, a rigid pipe is sleeved outside the capillary pipeline, one end of the rigid pipe is fixedly connected with the sample injection head, and the other end of the rigid pipe is fixedly connected with the air bag cavity; the rigid pipe is provided with an opening at the position corresponding to the first electromagnetic valve, so that the first electromagnetic valve controls the flow and the blockage of the capillary pipeline.
Furthermore, the test box body is cuboid, and four corners of the test box body are designed in an arc shape; the test box body comprises a test box body and a test box cover body matched with the test box body.
Furthermore, a rigid pipe is sleeved outside the capillary pipeline (4-2), one end of the rigid pipe is fixedly connected with the sample injection head (4-1), and the other end of the rigid pipe is fixedly connected with the air bag cavity (4-3); the rigid pipe is provided with an opening at the position corresponding to the first electromagnetic valve (8), so that the first electromagnetic valve (8) controls the circulation and the blockage of the capillary pipeline (4-2).
Further, the rigid pipe is made of transparent materials.
Furthermore, the sample inlet is of a conical structure.
Based on the portable hemorheology instrument test box for micro detection, the invention also provides a hemorheology detection method, a detection sample is added into the sample introduction head, a rubber plug of a gas bag in a gas bag cavity in the detection piece is aligned with the puncture needle, the puncture needle pierces the rubber plug, the detection piece is embedded into the test box body, the first electromagnetic valve controls the capillary pipeline to be blocked, the micro pump controls the pressure in the pressure supply pipeline under the condition that the pressure supply pipeline is circulated, the control detection module detects the pressure in the pipeline, when the pressure reaches the requirement, the second electromagnetic valve controls the pressure supply pipeline to be blocked, the micro pump stops working, the capillary heating groove heats the capillary pipeline, after the preset temperature is obtained, the first electromagnetic valve controls the capillary pipeline to be circulated, and simultaneously, the control detection module detects the pressure change in the pipeline, and calculating the viscosity value of the detection sample through the pressure change curve.
The invention has the beneficial effects that: the portable hemorheological instrument test box for trace detection and the adaptive detection method effectively solve the problems of large sample consumption, complex operation and the like of the conventional hemorheological detection, realize small equipment volume and convenient carrying, and are suitable for small hospitals, clinics and outgoing clinics.
Drawings
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention will be further described in detail with reference to the accompanying drawings, in which:
FIG. 1 is a perspective view of a body of the test cassette of the present invention;
FIG. 2 is a perspective view of a test element of the present invention;
FIG. 3 is an overall structure diagram of the portable blood rheometer test box for micro-amount testing according to the invention;
FIG. 4 is a schematic view of the internal structure of the test cassette of the present invention.
Wherein, 1 is a test box body, 1-2 is a test box cover body, 2 is a printer, 3-1 is a charging connector opening, 3-2 is a data transmission opening, 4 is a detection piece, 4-1 is a sample injection connector, 4-2 is a capillary pipeline, 4-3 is a capsule cavity, 6 is a control detection module, 7 is a micro pump, 8 is a first electromagnetic valve, 9 is a second electromagnetic valve, 10 is a puncture needle base, and 11 is a puncture needle.
Detailed Description
The present invention will be described in further detail with reference to specific examples.
It should be noted that these examples are only for illustrating the present invention, and not for limiting the present invention, and the simple modification of the method based on the idea of the present invention is within the protection scope of the present invention.
Referring to fig. 1-4, a portable blood rheometer test box for micro-detection comprises a test box body 1 and a detection piece 4 movably embedded on the upper surface of the test box body 1, wherein a control detection module 6, a micropump 7, a first electromagnetic valve 8, a second electromagnetic valve 9 and a puncture needle base 10 are fixedly arranged on the bottom surface inside the test box body 1; a puncture needle 11 is vertically arranged on the puncture needle base 10, the needle tail of the puncture needle 11 is fixedly arranged in the puncture needle base 10, the needle tail of the puncture needle 11 is connected with a first joint of a three-way joint, a second joint in the three-way joint is connected with a control detection module 6 through a detection pipeline, and a third joint in the three-way joint is connected with a micro pump 7 through a pressure supply pipeline; the second electromagnetic valve 9 controls the circulation and the blockage of the pressure supply pipeline;
the detection part 4 comprises a sample injection head 4-1, a capillary pipeline 4-2 and an air bag cavity 4-3, wherein an air bag is arranged in the air bag cavity, and a rubber plug is arranged at the bottom of the air bag; the sample injection head 4-1 is provided with a sample injection port, the sample injection port is communicated with one end of a capillary pipeline 4-2, and the other end of the capillary pipeline 4-2 is communicated with an air bag; the puncture needle 11 pierces the rubber plug of the air bag, so that the detection piece 4 is movably embedded on the upper surface of the test box body 1;
the first electromagnetic valve 8 controls the flow and the blockage of the capillary pipeline 4-2;
the upper surface of the test box body 1 is provided with a capillary heating groove corresponding to the capillary channel 4-2 and along the direction of the capillary channel 4-2.
Preferably, a printer 2 is further fixedly arranged on the bottom surface inside the test box body 1, and the printer 2 is electrically connected with the control detection module 6; the upper surface of the test box body 1 is provided with a display opening of the printer 2.
More specifically, a storage battery 5 is fixedly arranged on the bottom surface in the test box body 1, and the storage battery 5 is electrically connected with the control detection module 6; the storage battery 5 is connected with a charging connector, a charging connector opening 3-1 is formed in the side edge of the test box body 1, and the charging connector is arranged at the position 3-1.
Specifically, the control detection module 6 is provided with a data transmission joint, the side of the test box body 1 is provided with a data transmission opening 3-2, and the data transmission joint is arranged at the data transmission opening 3-2; the control detection module 6 performs data transmission with external equipment through a data transmission joint or wireless transmission. The electric joint opening 3-1 and the data transmission opening 3-2 are arranged at the left part of the bottom edge of the side edge of the test box body 1.
As an implementation mode, a rigid tube is sleeved outside the capillary tube 4-2, one end of the rigid tube is fixedly connected with the sample injection head 4-1, and the other end of the rigid tube is fixedly connected with the air bag cavity 4-3; the rigid tube is provided with an opening at the position corresponding to the first electromagnetic valve 8, so that the first electromagnetic valve 8 controls the flow and the blockage of the capillary channel 4-2. The test piece 4 can be taken without causing the test sample to topple and the like due to insufficient rigidity of the capillary channel 4-2. The rigid tube is preferably of a transparent material. The sample inlet is of a conical structure.
Specifically, four corners of the test box body 1 are designed to be arc-shaped; the test box body 1 comprises a test box body 1-1 and a test box cover body 1-2 matched with the test box body 1-1.
Based on the portable blood rheometer test box for micro detection, the blood rheology detection method comprises the following steps: adding a detection sample into a sample introduction head 4-1, aligning a rubber plug of a gas bag in a gas bag cavity 4-3 in a detection piece 4 with a puncture needle 11, enabling the puncture needle 11 to pierce the rubber plug, embedding the detection piece 4 into a test box body 1, controlling the capillary pipeline 4-2 to be blocked by a first electromagnetic valve 8, controlling the pressure of a micro pump 7 under the condition of circulating of a pressure supply pipeline, controlling a detection module 6 to detect the pressure in the pipeline, controlling the pressure supply pipeline to be blocked by a second electromagnetic valve 9 when the pressure meets the requirement, stopping the micro pump 7, heating the capillary pipeline 4-2 by a capillary heating groove, controlling the capillary pipeline 4-2 to be circulated by the first electromagnetic valve 8 after a preset temperature is obtained, controlling the detection module 6 to detect the pressure change in the pipeline, and calculating the viscosity value of the detection sample through a pressure change curve.
Finally, it is noted that the above-mentioned embodiments illustrate rather than limit the invention, and that, while the invention has been described with reference to preferred embodiments thereof, it will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims.
Claims (10)
1. A portable hemorheology instrument test box for micro detection comprises a test box body (1) and a detection piece (4) movably embedded on the upper surface of the test box body (1), and is characterized in that a control detection module (6), a micropump (7), a first electromagnetic valve (8), a second electromagnetic valve (9) and a puncture needle base (10) are fixedly arranged on the bottom surface inside the test box body (1); a puncture needle (11) is vertically arranged on the puncture needle base (10), the needle tail of the puncture needle (11) is fixedly arranged in the puncture needle base (10), the needle tail of the puncture needle (11) is connected with a first connector of a three-way connector, a second connector of the three-way connector is connected with the control detection module (6) through a detection pipeline, and a third connector of the three-way connector is connected with the micropump (7) through a pressure supply pipeline; the second electromagnetic valve (9) controls the circulation and the blockage of the pressure supply pipeline;
the detection piece (4) comprises a sample injection head (4-1), a capillary pipeline (4-2) and an air bag cavity (4-3), an air bag is arranged in the air bag cavity, and a rubber plug is arranged at the bottom of the air bag; the sample injection head (4-1) is provided with a sample injection port, the sample injection port is communicated with one end of the capillary pipeline (4-2), and the other end of the capillary pipeline (4-2) is communicated with the air bag; the puncture needle (11) pierces a rubber plug of the air bag, so that the detection piece (4) is movably embedded in the upper surface of the test box body (1);
the first electromagnetic valve (8) controls the circulation and the blockage of the capillary pipeline (4-2);
the upper surface of the test box body (1) is provided with a capillary heating groove corresponding to the capillary pipeline (4-2) and along the pipeline direction of the capillary pipeline (4-2).
2. The portable blood rheometer test box for micro-detection according to claim 1, wherein a printer (2) is further fixedly arranged on the bottom surface inside the test box body (1), and the printer (2) is electrically connected with the control detection module (6); the upper surface of the test box body (1) is provided with a display opening of the printer (2).
3. The portable blood rheometer test box for microscale detection according to claim 1, wherein a storage battery (5) is further fixedly arranged on the bottom surface inside the test box body (1), and the storage battery (5) is electrically connected with the control detection module (6); the storage battery (5) is connected with a charging connector, a charging connector opening (3-1) is formed in the side edge of the test box body (1), and the charging connector is arranged at the position (3-1).
4. The portable blood rheometer test box for microscale testing according to claim 3, wherein the control and detection module (6) is provided with a data transmission joint, a data transmission opening (3-2) is formed in a side of the test box body (1), and the data transmission joint is arranged at the data transmission opening (3-2); and the control detection module (6) performs data transmission with external equipment through the data transmission joint or wireless transmission.
5. The portable blood rheometer test cassette for micro-measurement according to claim 4, wherein the electrical connector opening (3-1) and the data transmission opening (3-2) are provided to the left of the side bottom edge of the test cassette body (1).
6. The portable blood rheometer test box for micro-detection according to claim 1, wherein a rigid tube is sleeved outside the capillary tube (4-2), one end of the rigid tube is fixedly connected with the sample injection head (4-1), and the other end of the rigid tube is fixedly connected with the air bag cavity (4-3); the rigid pipe is provided with an opening at the position corresponding to the first electromagnetic valve (8), so that the first electromagnetic valve (8) controls the circulation and the blockage of the capillary pipeline (4-2).
7. The portable blood rheometer test cassette for micro-scale testing as claimed in claim 6, wherein the rigid tube is made of a transparent material.
8. The portable blood rheometer test cassette for micro-scale detection as claimed in claim 1, wherein the sample inlet is of a cone-shaped configuration.
9. The portable blood rheometer test cassette for micro-scale detection according to claim 1, wherein the test cassette body (1) is a cuboid, and four corners of the test cassette body (1) are designed to be arc-shaped; the test box body (1) comprises a test box body (1-1) and a test box cover body (1-2) matched with the test box body (1-1).
10. A blood rheology detection method, characterized in that, based on claim 1 the portable hemorheometer test box for trace detection, add the detection sample into the sample introduction head (4-1), align the rubber plug of the air bag in the air bag cavity (4-3) in the detection piece (4) with the puncture needle (11), the puncture needle (11) pierces the rubber plug, imbed the detection piece (4) on the test box body (1), the first solenoid valve (8) controls the occlusion of the capillary pipeline (4-2), under the condition that the pressure supply pipeline circulates, the micro pump (7) controls the pressure, the control detection module (6) detects the pressure in the pipeline, when the pressure reaches the requirement, the second solenoid valve (9) controls the occlusion of the pressure supply pipeline, and the micro pump (7) stops working at the same time, the capillary heating groove heats the capillary pipeline (4-2), after the preset temperature is obtained, the first electromagnetic valve (8) controls the capillary pipeline (4-2) to circulate, the detection module (6) is controlled to detect the pressure change in the pipeline, and the viscosity value of the detection sample is calculated through the pressure change curve.
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Citations (5)
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US20050255601A1 (en) * | 2004-02-27 | 2005-11-17 | Medronic | Blood coagulation test cartridge, system, and method |
CN101813599A (en) * | 2010-04-20 | 2010-08-25 | 蔡泳 | Blood viscosity fast detection device and method thereof |
CN101819124A (en) * | 2009-02-27 | 2010-09-01 | 北京普利生仪器有限公司 | Method for automatically measuring blood viscosity |
CN204932404U (en) * | 2015-09-19 | 2016-01-06 | 李静 | Endocrinopathy effusion drainage device |
CN112932480A (en) * | 2019-11-26 | 2021-06-11 | 武汉普渡生物医药有限公司 | Finger blood trace quantitative sampling device and analysis method for rapid quantitative detection |
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2021
- 2021-10-28 CN CN202111279631.8A patent/CN113984584B/en active Active
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
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US20050255601A1 (en) * | 2004-02-27 | 2005-11-17 | Medronic | Blood coagulation test cartridge, system, and method |
CN101819124A (en) * | 2009-02-27 | 2010-09-01 | 北京普利生仪器有限公司 | Method for automatically measuring blood viscosity |
CN101813599A (en) * | 2010-04-20 | 2010-08-25 | 蔡泳 | Blood viscosity fast detection device and method thereof |
CN204932404U (en) * | 2015-09-19 | 2016-01-06 | 李静 | Endocrinopathy effusion drainage device |
CN112932480A (en) * | 2019-11-26 | 2021-06-11 | 武汉普渡生物医药有限公司 | Finger blood trace quantitative sampling device and analysis method for rapid quantitative detection |
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Title |
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