CN113980927B - 抗葡萄灰霉病和霜霉病相关蛋白chs1及其编码基因和应用 - Google Patents
抗葡萄灰霉病和霜霉病相关蛋白chs1及其编码基因和应用 Download PDFInfo
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1025—Acyltransferases (2.3)
- C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
- C12N9/1037—Naringenin-chalcone synthase (2.3.1.74), i.e. chalcone synthase
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- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C12N15/8282—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for fungal resistance
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- C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
- C12Y203/01074—Naringenin-chalcone synthase (2.3.1.74), i.e. chalcone synthase
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Abstract
Description
引物名称 | 序列(5’-3’) | 用途 | 序列表中的编号 |
CHS1-F | ATGGTGTCAGTGGGGGAAAT | 基因扩增 | SEQIDNO.6 |
CHS1-R | TGCTACACAATCGACTCAC | 基因扩增 | SEQIDNO.7 |
CHS1-qF | CGTTCTGAGCGAGTATGGGA | 实时荧光定量PCR | SEQIDNO.8 |
CHS1-qR | TGTGGTGCCCTTTCCTTCTT | 实时荧光定量PCR | SEQIDNO.9 |
EF-1α-F | AATTTTGACCAAGATCGACAGG | 葡萄内参基因 | SEQIDNO.10 |
EF-1α-R | CAGCAACAGTTTGACGCATG | 葡萄内参基因 | SEQIDNO.11 |
CHS1-2F | GCTCTAGAATGGTGTCAGTGGGGGAAATC | 亚细胞定位 | SEQIDNO.12 |
CHS1-2R | GCCCCGGGATGTGAGTCGATTGTGTAGCA | 亚细胞定位 | SEQIDNO.13 |
Claims (3)
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CN202111386527.9A CN113980927B (zh) | 2021-11-22 | 2021-11-22 | 抗葡萄灰霉病和霜霉病相关蛋白chs1及其编码基因和应用 |
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CN202111386527.9A CN113980927B (zh) | 2021-11-22 | 2021-11-22 | 抗葡萄灰霉病和霜霉病相关蛋白chs1及其编码基因和应用 |
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CN113980927A CN113980927A (zh) | 2022-01-28 |
CN113980927B true CN113980927B (zh) | 2023-08-11 |
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Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110734481A (zh) * | 2019-11-12 | 2020-01-31 | 中国农业大学 | 番茄SlMIP蛋白及其编码基因在调控植物灰霉病抗性中的应用 |
CN111118042A (zh) * | 2020-01-08 | 2020-05-08 | 西北农林科技大学 | 抗白粉病的葡萄钙依赖蛋白激酶基因VpCDPK9及其应用 |
WO2020126500A1 (en) * | 2018-12-17 | 2020-06-25 | Enza Zaden Beheer B.V. | Lettuce plant resistant to downy mildew and resistance gene |
CN113005129A (zh) * | 2021-01-25 | 2021-06-22 | 山东农业大学 | HrpZ_K基因在调控植物超敏反应性、抗病性和防治葡萄霜霉病中的应用及方法 |
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- 2021-11-22 CN CN202111386527.9A patent/CN113980927B/zh active Active
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2020126500A1 (en) * | 2018-12-17 | 2020-06-25 | Enza Zaden Beheer B.V. | Lettuce plant resistant to downy mildew and resistance gene |
WO2020125928A1 (en) * | 2018-12-17 | 2020-06-25 | Enza Zaden Beheer B.V. | Lettuce plant resistant to downy mildew and resistance gene |
CN110734481A (zh) * | 2019-11-12 | 2020-01-31 | 中国农业大学 | 番茄SlMIP蛋白及其编码基因在调控植物灰霉病抗性中的应用 |
CN111118042A (zh) * | 2020-01-08 | 2020-05-08 | 西北农林科技大学 | 抗白粉病的葡萄钙依赖蛋白激酶基因VpCDPK9及其应用 |
CN113005129A (zh) * | 2021-01-25 | 2021-06-22 | 山东农业大学 | HrpZ_K基因在调控植物超敏反应性、抗病性和防治葡萄霜霉病中的应用及方法 |
Non-Patent Citations (1)
Title |
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青花菜查尔酮合成酶基因 BoCHS 的克隆、表达及序列分析;彭礼琼;《湖 北 农 业 科 学》;第49卷(第8期);1796-1800 * |
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