CN113969217A

CN113969217A - Cleaning composition and use thereof

Info

Publication number: CN113969217A
Application number: CN202010837859.3A
Authority: CN
Inventors: 蔡昌廷; 陈劲中; 曾怡静; 郑新耀; 蔡承佳
Original assignee: CPC Corp Taiwan
Current assignee: CPC Corp Taiwan
Priority date: 2020-07-22
Filing date: 2020-08-19
Publication date: 2022-01-25
Also published as: JP2022022042A; TWI764208B; JP7062733B2; TW202204585A

Abstract

A cleaning composition comprises a rice bran enzyme crude extract, a surfactant and a preservative, wherein the rice bran enzyme crude extract is prepared by the following steps: providing rice bran and enzyme extraction solution; mixing the rice bran and the enzyme extract solution in a ratio of 1:8 to 1:2, stirring and centrifuging to obtain rice bran lipase enzyme extract; gradually adding ammonium sulfate into the rice bran lipase enzyme extract, and continuously stirring to obtain a rice bran lipase enzyme solution; and adding an alcohol water solution into the rice bran lipase enzyme solution, stirring and standing, and then carrying out centrifugation or gravity settling to obtain the rice bran lipase crude extract. A method of cleaning comprising the step of contacting the above cleaning composition with laundry to be cleaned.

Description

Cleaning composition and use thereof

Technical Field

The invention relates to a cleaning composition, in particular to a cleaning composition containing a rice bran enzyme crude extract; in addition, the invention also relates to a cleaning method using the cleaning composition.

Background

In the prior art, industrial enzymes such as protease, amylase and lipase are widely applied to industries such as food, feed, paper making, textile, detergent, health care products and biomass energy. The enzyme is applied to the cleaning agent, which is started from 1913 for the first time, trypsin extracted from pig pancreas is added into a Bumus cleaning agent by Rohm company in Germany, and protein stains of human bodies and food are decomposed by the protease. However, since trypsin is inactivated in alkaline environment, Novo Nordisk of Denmark and Gist-Brocade of the Netherlands have developed respectively the addition of alkali-resistant serine protease (alkaline serine protease) derived from Bacillus licheniformis (Bacillus licheniformis) to detergents to prepare detergents having the product names Alcal and Maxase, respectively, so that they exhibit high washing activity under alkaline conditions.

Subsequently, in the 1970 s, detergent developers in europe attempted to add amylase to detergent formulations, which aided in the removal of spots formed on laundry by food residues such as chocolate, by the property of amylase to hydrolyze alpha-1, 4-glucosidic bonds in starch-based soils, and by the anti-redeposition effect of iron ions in water and other types of soils contaminated with starch. However, amylase has an optimal activity at a pH of 6 to 7, and the most suitable temperature is 75 ℃ to 85 ℃, so amylase is only suitable for high temperature washing, not for low temperature washing environments.

In order to solve the problem that the amylase cannot be used for washing clothes in a low-temperature environment, in 1987, the Japanese lion king company adds lipase into a washing powder formula, and because the lipase can decompose sebum dirt on collars and cuffs and lipid dirt on food stains into glycerol and fatty acid, the sebum dirt and the lipid dirt can be easily emulsified and removed by a surfactant. However, since lipase acts on the oil-water interface, it is necessary to perform washing several times to exert the washing effect, and it is difficult to obtain the detergency effect of lipase after one washing.

In addition, in the case of a liquid detergent containing a large amount of water, the polar solvent and the charged surfactant also destroy the tertiary structure of the enzyme, resulting in denaturation of the protein, thereby degrading and inactivating the enzyme.

The rice bran is a product of a process of grinding the brown rice into white rice, and accounts for about 8 to 10% of the total weight of the brown rice, and about 12 to 15 ten thousand tons of rice bran can be produced by completely grinding the brown rice into white rice in taiwan, based on the yield of rice of about 150 to 170 ten thousand tons per year. However, since rice bran is a by-product having a very low economic value, it is often discarded as agricultural waste.

Therefore, it is an urgent need in the art to develop a stable enzyme cleaning composition having strong cleaning ability at room temperature, high temperature resistance, and organic solvent resistance by using rice bran, which is regarded as agricultural waste.

Disclosure of Invention

In order to effectively utilize rice bran, which is regarded as agricultural waste, the present invention provides a cleaning composition comprising a rice bran enzyme crude extract.

To achieve the above and other objects, the present invention provides a cleaning composition comprising a rice bran enzyme crude extract, a surfactant and a preservative, wherein the rice bran enzyme crude extract is prepared by the following steps:

providing rice bran and an enzyme extraction solution, wherein the enzyme extraction solution comprises distilled water and Triton X-100;

mixing the rice bran and the enzyme extract solution in a ratio of 1:8 to 1:2, stirring and centrifuging to obtain rice bran lipase enzyme extract;

gradually adding ammonium sulfate into the rice bran lipase enzyme extract until the concentration of ammonium sulfate in the rice bran lipase enzyme extract reaches 40 wt% to 60 wt%, and continuously stirring to obtain a rice bran lipase enzyme solution; and

adding 20 wt% to 50 wt% of alcohol water solution into the rice bran lipase enzyme solution, stirring and standing, centrifuging or gravity settling to obtain the rice bran enzyme crude extract,

wherein the content of the rice bran ferment crude extract ranges from 0.4 wt% to 20 wt% based on the total weight of the cleaning composition.

The cleaning composition of the present invention, wherein the amount of Triton X-100 is between 0.01 wt% and 1 wt% based on the total weight of the enzyme extract solution.

The content of the rice bran ferment crude extract in the cleaning composition may range from 0.5 wt% to 15 wt% based on the total weight of the cleaning composition.

The content of the rice bran ferment crude extract in the cleaning composition may range from 5 wt% to 10 wt% based on the total weight of the cleaning composition.

The content of the rice bran ferment crude extract in the cleaning composition may range from 0.5 wt% to 2 wt% based on the total weight of the cleaning composition.

The above cleaning composition, wherein the surfactant is selected from the group consisting of sodium lauryl sulfate, sodium dodecylbenzene sulfonate, sodium dodecylpolyoxyethylether sulfate, alpha-alkenyl sodium sulfonate, methyl polyglycol ether fatty acid ester, lauryl dimethyl amine oxide, and fatty alcohol polyoxyethylene ether.

The content of the surfactant in the cleaning composition may range from 4 wt% to 15 wt%, based on the total weight of the cleaning composition.

In the above cleaning composition, the preservative may be selected from the group consisting of methylparaben (methylparaben), phenoxyethanol (phenoxyethanol), benzoic acid (benzoic acid), and sodium benzoate (sodium benzoate).

The content of the preservative in the cleaning composition may range from 0.2 wt% to 0.6 wt%, based on the total weight of the cleaning composition.

The cleaning composition, wherein the cleaning composition further comprises a stabilizer.

The above cleaning composition, wherein the stabilizer comprises triethanolamine, sorbitol, 1, 2-propanediol, citric acid, sodium citrate, calcium chloride dihydrate, glycine, and glycerin.

The cleaning composition, wherein the content of the 1, 2-propylene glycol can range from 1.5 wt% to 6 wt%, the content of the citric acid can range from 0.01 wt% to 6 wt%, the content of the sodium citrate can range from 0.1 wt% to 6 wt%, the content of the calcium chloride dihydrate can range from 0.05 wt% to 0.5 wt%, the content of the glycine can range from 0.5 wt% to 4 wt%, and the content of the glycerin can range from 1.5 wt% to 6 wt%, based on the total weight of the cleaning composition.

To achieve the above and other objects, the present invention further provides a cleaning method comprising the step of contacting the above cleaning composition with laundry to be cleaned.

The cleaning percentage of the clothes cleaned by the cleaning composition is obviously superior to that of collar essence of Miaobute householders and Maobao ferment collar essence sold in the market.

In the embodiments of the present invention, it was confirmed that the cleaning composition comprising 0.5%, 1%, 2%, 5% or 10% rice bran enzyme crude extract provides superior washing ability to commercially available wool treasure enzyme collar essence for soiled towels, soiled cloths made of white cotton-hemp mixed fabrics, or commercially available standard soiled cloths, stained with oily stains such as fantastic pens or lipstick, stained with sauce such as mustard, stained with stains such as ink insoluble in dry cleaning solvents, and the like, without rubbing the soiled cloths.

In addition, in other embodiments of the present invention, it is also confirmed that the rice bran enzyme crude extract provided by the present invention has stability effects against high temperature and organic solvent. Therefore, the invention successfully obtains the rice bran enzyme crude extract from the rice bran, prepares the cleaning composition with the cleaning capability superior to that of the collar essence of commercially available wonderman and Maobao enzyme collar essence, and solves the problems that the enzyme cleaner in the prior art cannot provide strong cleaning capability, high temperature resistance, stability of organic solvent resistance and the like at normal temperature. In addition, because the rice bran is often used as agricultural waste, the invention successfully recycles the agricultural waste, and simultaneously achieves the purposes of permanently utilizing resources, saving energy and reducing carbon.

Drawings

FIG. 1 shows the cleaning effect of the cleaning composition of comparative example A and example A-1, commercially available Saikoujia collar extract, Saikoujia collar extract added with 1% rice bran ferment crude extract powder, and commercially available Maobaojia ferment collar extract on stains such as lipstick, mustard sauce, ink on white towels;

FIG. 2 shows the cleaning effect of the cleaning compositions of comparative example B, example B-1 and example B-2 and the commercially available Maobao enzyme collar essence on stains such as wonderful pen or ink on the white ramie mixed fabric;

FIG. 3 shows the cleaning effect of the cleaning compositions of comparative example B and example B-2 of the present invention and the commercially available Maobao enzyme collar essence on the commercially available standard soiled cloth such as JB-02 protein soiled cloth or JB-03 sebum soiled cloth;

FIG. 4 shows the cleaning effect of the cleaning compositions of comparative example B, example B-3 and example B-4 and the commercially available Maobao enzyme collar essence of the present invention on stains such as lipstick or ink on white hemp blended fabric after washing with clean water;

FIG. 5 shows the cleaning effect of the cleaning composition of comparative example B and example B-3, commercially available Maobao ferment collar essence, and Maobao ferment collar essence added with 0.5% rice bran ferment coarse extract powder after washing with clean water on stains such as lipstick or ink on white ramie mixed fabric;

FIG. 6 shows the cleaning effect of the cleaning compositions of comparative example B, example B-4 and example B-5 and the commercially available Maobao enzyme collar essence of the present invention on stains such as lipstick or ink on white hemp blended fabric after washing with clean water;

FIG. 7 shows the cleaning effect of the cleaning compositions of comparative example B and example B-4 and commercially available Maobao enzyme collar essence of the present invention after washing with Korea laundry detergent on stains such as lipstick or ink on white cotton-flax mixed fabric;

FIG. 8 shows the cleaning effect of the cleaning compositions of comparative example B, example B-4 and example B-5 and the commercially available Maobao enzyme collar essence of the present invention on lipstick stains on white cotton-flax blended fabric after washing with the Korea laundry essence;

FIG. 9 shows the cleaning effect of the cleaning compositions, commercially available laundry detergents to which 2% rice bran enzyme crude extract is added, or commercially available Persil laundry detergents on stains such as lipstick or ink on white cotton-flax mixed fabrics according to comparative example C, example C-1, example C-2, and example C-3 of the present invention;

FIG. 10 shows the cleaning effect of the cleaning compositions of comparative example C, example C-2, example C-3 and example C-4, commercially available laundry detergent with 3% rice bran enzyme crude extract added thereto, or commercially available Persil detergent on stains such as wonderpen or ink on white linen fabrics;

FIG. 11 shows the enzyme activity of the cleaning composition of comparative example B after mixing with stabilizer formulation D1 and standing at 4 ℃ and 45 ℃ for one to six weeks;

FIG. 12 is an observation of delamination after one week of standing at 45 ℃ after mixing the cleaning composition of comparative example B of the present invention with different stabilizer formulations;

FIG. 13 shows the cleaning effect of the cleaning compositions of comparative example E and example E-1 and the commercially available Maobao enzyme collar-treated white ramie mixed fabric after 5 minutes of stains such as wonderful pen, lipstick or ink, and then washed with clean water;

FIG. 14 shows the cleaning effect of the laundry detergent after washing the white linen-wool blended fabric with the cleaning composition of comparative example E and example E-1 of the present invention and the commercially available Maobao enzyme collar fine treated stains such as wonder, lipstick or ink for 5 minutes;

FIG. 15 shows the cleaning effect of the cleaning compositions of comparative example E and example E-1 of the present invention and the commercially available Maobao enzyme collar-treated white ramie mixed fabric after 20 minutes of stains such as wonderful pen, lipstick or ink, and washing with clean water;

FIG. 16 shows the cleaning effect of the laundry detergent after washing the white linen-wool blended fabric with the cleaning composition of comparative example E and example E-1 of the present invention and the commercially available Maobao enzyme collar fine treated stains such as wonders, lipsticks or ink for 20 minutes;

FIG. 17 shows lipase activities after the Collar extract formulation F is matched with different enzyme stabilizer formulations, and then different enzymes are added and placed at 45 ℃ for 1 week and 13 weeks;

FIG. 18 shows lipase activities after the collar extract formulation F is matched with different enzyme stabilizer formulations, and then different enzymes are added and placed at 45 ℃ for 3 weeks, 5 weeks and 13 weeks;

FIG. 19 shows lipase activities after the Collar extract formulation F is matched with different enzyme stabilizer formulations, and then different enzymes are added and placed at 45 ℃ for 1 week and 49 weeks; and

FIG. 20 shows the lipase activities of collar extract formulation F after mixing with different enzyme stabilizer formulations and adding different enzymes at 25 deg.C for 1 week and 49 weeks.

Reference numerals

F0 example F-0

F1 example F-1

F2 example F-2

F3 example F-3

F4 example F-4

1 standing for 1 week

3 standing for 3 weeks

5 standing for 5 weeks

13 standing for 13 weeks

49 standing for 49 weeks

Lo Lipolase

Lx Lipex

LoM Lipolase+MDA

LxM Lipex+MDA

R RBL

RM RBL+MDA

RL RBL+Lipolase

Detailed Description

The following description of the embodiments of the present invention is provided by way of specific examples, and other advantages and effects of the present invention will be apparent to those skilled in the art from the disclosure of the present specification. The invention is capable of other and different embodiments and its several details are capable of modification in various other respects, all without departing from the spirit and scope of the present invention.

As used in the specification and the appended claims, the singular forms "a", "an", and "the" include plural referents unless the context clearly dictates otherwise.

As used in the specification and the appended claims, the term "or" includes "and/or" is used in its sense unless the context clearly dictates otherwise.

Triton X-100 used in the present invention is a nonionic surfactant having IUPAC name of 2- [4- (2,4, 4-trimetylpentan-2-yl) phenoxy]ethanol (2- [4- (2,4, 4-trimethylpent-2-yl) phenoxy group]Ethanol) of the formula C₁₄H₂₂O(C₂H₄O)_n。

The invention provides a cleaning composition containing a rice bran enzyme crude extract, wherein the rice bran enzyme crude extract is prepared by the following steps: adding rice bran into the enzyme extraction solution according to different proportions of the rice bran and the enzyme extraction solution, stirring for 30-60 minutes, and centrifuging to obtain a rice bran lipase enzyme extract, wherein the proportion of the rice bran and the enzyme extraction solution is 1: 8-1: 2, and the enzyme extraction solution comprises distilled water and Triton X-100 (the content of the Triton X-100 is 0.01 wt% to 1 wt% based on the total weight of the enzyme extraction solution). The first stage of the rice bran lipase purification comprises gradually adding ammonium sulfate to the rice bran lipase enzyme extract until the concentration of ammonium sulfate in the rice bran lipase enzyme extract reaches 40 wt% to 60 wt%, and continuously stirring for 30 to 60 minutes to obtain a rice bran lipase enzyme solution completed in the first stage of the rice bran lipase purification. The second stage of rice bran lipase purification comprises adding the obtained rice bran lipase enzyme solution into an aqueous alcohol solution, stirring for 30-60 minutes, standing for 10 minutes, and then centrifuging or gravity settling to obtain rice bran lipase purified enzyme precipitate, wherein the concentration of the alcohol is 20-50 wt%, so as to obtain the rice bran lipase crude extract. The content of the rice bran ferment crude extract is between 0.4 wt% and 20 wt% based on the total weight of the cleaning composition. In one embodiment, the crude rice bran enzyme extract comprises lipase and esterase.

In one embodiment, the rice bran enzyme crude extract is at least 0.4 wt%, 0.5 wt%, 1 wt%, 1.2 wt%, 1.5 wt%, 1.8 wt%, 2 wt%, 2.5 wt%, 3 wt%, 4 wt%, 5 wt%, 8 wt%, or 10 wt%, and the rice bran enzyme crude extract is at most 20 wt%, 18 wt%, 16 wt%, 15 wt%, 14 wt%, 12 wt%, or 10 wt%, based on the total weight of the cleaning composition.

In one embodiment, the rice bran ferment crude extract is present in an amount ranging from 0.5 wt% to 15 wt% based on the total weight of the cleaning composition. In another embodiment, the rice bran ferment crude extract is present in an amount ranging from 1 wt% to 12 wt% based on the total weight of the cleaning composition. In another embodiment, the rice bran ferment crude extract is present in an amount ranging from 2 wt% to 10 wt% based on the total weight of the cleaning composition. In another embodiment, the rice bran ferment crude extract is present in an amount ranging from 5 wt% to 8 wt% based on the total weight of the cleaning composition. In another embodiment, the rice bran ferment crude extract is present in an amount ranging from 0.5 wt% to 2 wt% based on the total weight of the cleaning composition.

In one embodiment, the cleaning composition further comprises a surfactant and a preservative, wherein the surfactant includes, but is not limited to, sodium lauryl sulfate (sodium lauryl sulfate), sodium dodecyl benzene sulfonate (sodium dodecyl benzene sulfonate), sodium dodecyl polyoxyethylene ether sulfate (sodium lauryl sulfate), sodium alpha-olefin sulfonate (sodium alpha-olefin sulfonate), polyethylene glycol ether fatty acid methyl ester, lauryl amine oxide (lauryl dimethyl amine oxide), fatty alcohol polyoxyethylene ether (fatty alcohol ethoxylates), or combinations thereof, and the preservative includes, but is not limited to, methyl paraben (methyl paraben), phenoxyethanol (phenoxy ethanol), benzoic acid (benzoic acid), sodium benzoate (sodium benzoate), or combinations thereof. In one embodiment, the surfactant is present in an amount ranging from 4 wt% to 15 wt% and the preservative is present in an amount ranging from 0.2 wt% to 0.6 wt%, based on the total weight of the cleaning composition.

In one embodiment, the cleaning composition further comprises a stabilizer, wherein the stabilizer comprises triethanolamine, sorbitol, 1, 2-propanediol, citric acid, sodium citrate, calcium chloride dihydrate, glycine, and glycerin. In one embodiment, based on the total weight of the cleaning composition, the 1, 2-propanediol is present in an amount ranging from 1.5 wt% to 6 wt%, the citric acid is present in an amount ranging from 0.01 wt% to 6 wt%, the sodium citrate is present in an amount ranging from 0.1 wt% to 6 wt%, the calcium chloride dihydrate is present in an amount ranging from 0.05 wt% to 0.5 wt%, the glycine is present in an amount ranging from 0.5 wt% to 4 wt%, and the glycerol is present in an amount ranging from 1.5 wt% to 6 wt%.

In one embodiment, the cleaning composition is a liquid. In one embodiment, the cleaning composition is a laundry detergent or a collar detergent.

Preparation example 1 preparation of crude extract of rice bran enzyme

The method for extracting the crude rice bran ferment extract of the preparation example comprises the following steps:

mixing rice bran and enzyme extraction solution in a ratio of 1: 4 for 60 minutes, and separating the mixture in a continuous centrifuge to obtain a rice bran lipase enzyme extract solution, wherein the enzyme extract solution consists of 99.9 wt% of distilled water and 0.1 wt% of Triton X-100. Thereafter, ammonium sulfate was gradually added to the rice bran lipase enzyme extract until the concentration of ammonium sulfate in the rice bran lipase enzyme extract reached 60 wt%, and stirred for 60 minutes to obtain a rice bran lipase enzyme solution. Finally, 50 wt% of an aqueous alcohol solution was added to the rice bran lipase enzyme solution, stirred for 60 minutes and left to stand for 10 minutes, and then settled by gravity, and finally the precipitate was freeze-dried to obtain a rice bran enzyme crude extract powder.

Preparation example 2 preparation of cleaning composition

The rice bran enzyme crude extract powder prepared in preparation example 1 was mixed with a surfactant, a preservative and water to prepare the cleaning composition of the present invention. Examples of the cleansing composition of the present invention are shown in tables 1 and 2 below, wherein the cleansing composition of table 1 can be used as a collar essence; and the cleaning compositions of table 2 can be used as laundry detergents. In addition, table 1 and table 2 also provide a comparative example without rice bran ferment crude extract powder as a control.

TABLE 1 Collar extract formulation of cleaning composition

Table 2 laundry detergent formulations of cleaning compositions

Preparation example 3 preparation of a stabilizer for crude extract of Rice bran enzyme

Different weight percentages of 1, 2-propanediol, sodium citrate, calcium chloride dihydrate, glycine, and glycerin were mixed to prepare a stabilizer formulation for the cleaning composition, the stabilizer formulation prepared being shown in table 3 below.

TABLE 3 stabilizing agent formulation for crude extract of rice bran enzyme

	Formulation D1 (wt%)	Formulation D2 (wt%)	Formulation D3 (wt%)
				1, 2-propanediol	5	2.5	2.5
Citric acid sodium salt	5	2.5	5
				Calcium chloride dihydrate	0.1	0.1	0.1
Glycine	3	1.5	3
				Glycerol	5	2.5	2.5

Preparation example 4 preparation of self-soiled Fabric

The white towel or white cotton-flax mixed fabric of 8 cm × 5 cm is smeared with stains such as lipstick (one pass and repeated), 200 μ L of mustard paste or 100 μ L of ink, respectively, and left to stand for 1 hour.

Test mode 1 numerical analysis of stain cleaning Effect

A Canon EOS 60D camera was used in conjunction with a photographic light box with Light Emitting Diode (LED) light sources in three directions to take pictures, the three light sources of the light box were adjusted to the maximum, and a cleaning test sample was placed in the box and taken pictures were taken under conditions of light sensitivity ISO 100, aperture 8.0, and shutter 1/200 seconds. And then, performing image analysis before and after cleaning the stains of the test samples by using image adjusting software (Photoshop), respectively analyzing a white block (with the highest brightness value) and a stain block (with the lowest brightness value) of each sample, and subtracting the brightness values of the white block and the stain block to obtain a relative brightness value. The cleaning performance is expressed as the stain cleaning percentage, which is obtained by the following formula:

test mode 2 enzyme activity analysis of crude rice bran enzyme extract

18.45 ml of 1M K₂HPO₄With 11.55 ml of 1M KH₂PO₄Mixed and deionized water was added to a total volume of 300 ml to prepare 0.1M potassium phosphate buffer solution (pH 7). 40 mg of p-nitrophenylpalmitate (p-NPP) was dissolved in 12 ml of isopropanol to prepare an 8.8mM solution of p-NPP (i.e., solution A) and stored at-30 ℃ protected from light. 0.8889 g of Triton X-100 was mixed with 0.2222 g of gum arabic (gum arabic) in 200 ml of 0.1M potassium phosphate buffer solution to prepare solution B. After mixing 0.2 ml of solution a with 3 ml of solution B, shaking and thoroughly mixing to obtain a p-NPP matrix solution.

0.1 ml of the appropriately diluted sample to be tested is mixed with 3.2 ml of the p-NPP matrix solution,after shaking in a 35 ℃ water bath for 20 minutes, the enzyme reaction was stopped by boiling in a boiling water bath for 5 minutes. Centrifuging at 6000rpm for 10 min, collecting supernatant and determining OD₄₀₀The absorbance of (a).

Test example 1 test of detergency of cleaning composition

Test example 1 in order to test the washing ability of the cleaning composition containing rice bran enzyme crude extract, the cleaning compositions of comparative example a and example a-1, which were collar extracts, were tested using numerical analysis of the washing effect of stains, and the washing ability of white towels (self-made dirty cloths) stained with stains such as lipstick, mustard paste, or ink, were used as a positive control group, and a negative control group, which was washed with collar extracts of commercially available mao housekeeper and Maobao enzyme collar extracts. The cleaning method of this test example was a hand-kneading method of cleaning each soiled fabric, folding the soiled fabric in half, kneading the soiled fabric with hands 10 times by the same person, spreading the soiled fabric and rotating it at 90 degrees, folding the soiled fabric in half, kneading the soiled fabric with hands 10 times, and standing the soiled fabric at room temperature for 20 minutes. Soaking the dirty cloths in a beaker filled with 900 ml of clean water, stirring for 15 minutes at the rotating speed of 240rpm by using a stirrer, then pouring out the sewage, replacing 900 ml of clean water, and stirring for 15 minutes at the rotating speed of 240rpm so as to simulate the cleaning process of a washing machine. Finally, the sewage is poured out, a small amount of clear water is used for washing the dirty cloth, and then the dirty cloth is spread out, dried in the air and photographed and recorded. The results are shown in figure 1 and table 4, comparing

groups

1 and 2 and

groups

3 and 4, and show that the 1% rice bran enzyme crude extract significantly enhances the ability of the cleaning composition of comparative example a and the wonderman's collar to clean stains. In addition, the results of comparing groups 2 to 5 show that the cleaning ability of the cleaning composition of example a-1 is significantly better than the cleaning ability of the commercially available tubician collar extract, the tubician collar extract to which 1% rice bran enzyme crude extract powder is added, and the commercially available furnase collar extract. The results of this test example demonstrate that the cleaning composition comprising 1% rice bran enzyme crude extract provides superior cleaning ability to commercially available wonderman collar essence and wool treasure enzyme collar essence for self-made soiled cloth of white towels.

Table 4 comparison of cleaning percentages of the cleaning composition of comparative example a and example a-1, commercially available housekeeper collar essence, housekeeper collar essence added with 1% rice bran enzyme crude extract powder, and commercially available microbian enzyme collar essence for home-made white towel soiled cloth

Test example 2

Test example 2 in order to test the washing ability of the cleaning compositions containing rice bran enzyme crude extracts of 5% or 10% or the like at various concentrations, the cleaning compositions of comparative example B, example B-1 and example B-2, which are collar conditioners, were tested by numerical analysis of the washing effect of stains, for the washing ability of white cotton-hemp mixed cloth (self-made dirty cloth) stained with stains such as crayon or ink, or standard dirty cloth such as JB-02 protein dirty cloth or JB-03 sebum dirty cloth purchased from the institute of chemical industries of china, taiyuan, of shanxi, china, and the collar conditioner was commercially available as a positive control group and the clean water washing was used as a negative control group. The cleaning method of the test example is to clean with a vertical decontamination tester (Terg-o-meter), 1000 ml of clean water is filled in a glass cup of the vertical decontamination tester, each piece of soiled cloth is respectively put into the clean water, the clean water is stirred for 15 minutes in a forward rotation and a reverse rotation of 200rpm, the sewage is poured out, 1000 ml of clean water is replaced, and then the clean water is stirred for 15 minutes in a forward rotation and a reverse rotation of 200rpm to simulate the cleaning process of the washing machine. Finally, the sewage is poured out, a small amount of clear water is used for washing the dirty cloth, and then the dirty cloth is spread out, dried in the air and photographed and recorded. The results are shown in figure 2 and table 5, comparing groups 6 to 8, and show that 5% and 10% crude rice bran enzyme extracts significantly enhance the cleaning ability of the cleaning composition of comparative B for homemade soiled cloths. In addition, the results of comparing groups 7 to 10 show that the cleaning ability of the cleaning compositions of examples B-1 and B-2 is superior to that of the commercially available Maobao enzyme collar essence and water.

Table 5 compares the cleaning percentage of the cleaning compositions of comparative example B, example B-1 and example B-2, commercially available Maobao enzyme collar essence and water to the self-made soiled cloth of white ramie mixed fabric.

Also, as shown in fig. 3 and table 6, the results of comparing groups 11 and 12 show that the 10% rice bran enzyme crude extract significantly enhances the cleaning ability of the cleaning composition of comparative example B against standard soiled cloths. In addition, the results of comparing groups 12 to 14 show that the cleaning ability of the cleaning composition of example B-2 is superior to that of the commercially available Maobao enzyme collar essence and water. The results of this test example confirm that the cleaning composition containing 5% or 10% rice bran enzyme crude extract provides superior cleaning ability to the commercially available Maobao enzyme collar cleaner for the homemade dirty cloth of the white ramie mixed fabric or the commercially available standard dirty cloth without rubbing the dirty cloth.

TABLE 6 percentage of cleaning compositions, commercially available Maobao ferment Collar extract and Water, comparative example B and example B-2 to standard soiled cloths

Test example 3

Test example 3 in order to test the washing ability of the cleaning compositions containing rice bran enzyme crude extracts of various concentrations, such as 0.5%, 1%, or 2%, the washing ability of the cleaning compositions of comparative example B, example B-3, example B-4, and example B-5, with respect to the self-soiled cloths of white cotton hemp fabrics stained with stains, such as lipstick or ink, was tested by numerical analysis of the stain washing effect. The cleaning method of this embodiment is to spray the cleaning composition, Maobao ferment collar essence or clean water on the stains and to stand for 20 minutes, and then to place the stains in a vertical decontamination testing machine to be cleaned with clean water or to add laundry essence for cleaning.

The results of the cleaning with clean water, as shown in fig. 4 and table 7, for comparative groups 15 to 17, show that the 0.5% and 1% crude extracts of rice bran enzyme significantly enhance the cleaning ability of the cleaning composition of comparative example B for homemade soiled cloths. In addition, the results of comparing groups 16 to 19 show that the cleaning ability of the cleaning compositions of examples B-3 and B-4 is significantly better than that of the commercially available Maobao ferment collar essence and water.

Table 7 compares the cleaning percentages of the cleaning compositions of comparative example B, example B-3, and example B-4, commercially available Maobao enzyme Collar essence, and Water to clean Water-washed homemade soiled cloths

Also, as shown in fig. 5 and table 8, the cleaning ability of the cleaning composition of example B-3 was significantly better than that of commercially available napa enzyme collar extract, commercially available napa enzyme collar extract added with 0.5% rice bran enzyme crude extract, and water, as shown by comparing groups 20 to 24.

TABLE 8 percentage of clean-up for homemade soiled cloths washed with clean water compared with cleaning compositions of comparative example B and example B-3, commercially available Maobao enzyme collar essence supplemented with 0.5% crude rice bran enzyme extract, and Water

Also, as shown in fig. 6 and table 9, the results of comparing groups 25 to 27 show that 1% and 2% crude rice bran enzyme extracts significantly enhance the cleaning ability of the cleaning composition of comparative example B for homemade soiled cloths. In addition, the results of comparing groups 26 to 28 show that the cleaning ability of the cleaning compositions of examples B-4 and B-5 is significantly better than that of the commercially available Maobao enzyme collar essence and water.

TABLE 9 comparison of the percent cleaning of comparative example B, example B-4 and example B-5 cleaning compositions, commercially available Maobao enzyme Collar essence and Water on clean Water washed homemade soiled cloths

The results of cleaning with the laundry detergent, as shown in figure 7 and table 9, show that the 1% rice bran enzyme crude extract significantly enhances the cleaning ability of the cleaning composition of comparative example B for homemade soiled cloths by comparing groups 30 and 31. In addition, the results of comparing groups 31 to 33 show that the cleaning ability of the cleaning composition of example B-4 is significantly better than the cleaning ability of the commercially available Maobao enzyme collar essence and water.

TABLE 10 comparison of the cleaning percentages of the cleaning compositions of comparative example B and examples B-4, commercially available Maobao enzyme Collar essence, and Water for homemade soiled cloths laundered with Korea laundry essence

Also, as shown in fig. 8 and table 11, the results of comparing groups 34 to 36 show that 1% and 2% rice bran enzyme crude extracts significantly enhance the cleaning ability of the cleaning composition of comparative example B for homemade soiled cloths. In addition, the results of comparing groups 35 to 38 show that the cleaning ability of the cleaning compositions of examples B-4 and B-5 is significantly better than that of the commercially available Maobao ferment collar essence and water.

Table 11 compares the cleaning percentages of the cleaning compositions of comparative example B, example B-4, and example B-5, commercially available Maobao enzyme collar essence, and water, for homemade soiled cloths washed with Korea laundry essence.

The above results show that the cleaning composition containing 0.5%, 1% or 2% rice bran enzyme crude extract has better washing effect than commercially available Maobao enzyme collar extracts by treating stains with clean water or laundry detergent without rubbing dirty cloth. In addition, as shown by the results of groups 15 to 18, the cleaning ability of the self-made dirty cloth can be significantly improved by adding 0.5% or 1% of the rice bran enzyme crude extract, and as shown by the results of groups 26 and 27, and 35 and 36, the cleaning effect of the dirty cloth can be achieved by adding the rice bran enzyme crude extract with low concentration.

The results of test examples 1 to 3 confirmed that the cleaning ability of the cleaning composition containing the rice bran ferment crude extract as collar essence of the present invention is significantly better than that of the commercially available Maobao ferment collar essence, indicating that the rice bran ferment crude extract in the cleaning composition of the present invention provides excellent laundry cleaning effect.

Test example 4

Test example 4 in order to test the washing ability of the cleaning compositions containing rice bran enzyme crude extracts of various concentrations, such as 0.5%, 1%, 2% or 3%, the cleaning compositions of comparative example C, example C-1, example C-2 and example C-3, which are laundry detergents, and the commercially available Korea laundry detergent to which 2% rice bran enzyme crude extract is added were tested using numerical analysis of the stain washing effect, the washing ability of the white cotton hemp mixed fabric (self-made stain fabric) stained with lipstick or ink, and the commercially available Korea laundry detergent or the commercially available Persil laundry detergent was used as a positive control group, and the clean water washing was used as a negative control group. In the washing method of this example, each soiled cloth was immersed in each cleaning composition, commercially available kokura detergent added with 2% crude extract of rice bran enzyme, commercially available Persil detergent, or clean water, and was washed in a vertical stain removal tester. As shown in fig. 9 and table 12, the results of comparison of groups 39 to 42 show that 0.5%, 1% and 2% crude extracts of rice bran enzyme significantly enhance the cleaning ability of the cleaning composition of comparative example C for self-made soiled cloths, especially example C-2 containing 1% crude extract of rice bran enzyme provides better soil cleaning ability. In addition, the results of comparing groups 43 and 44 show that the 2% rice bran enzyme crude extract also enhances the cleaning ability of commercial laundry detergent for homemade soiled cloths. Furthermore, the results of comparing groups 39 to 46 show that the cleaning compositions of examples C-1, C-2 and C-3 have superior cleaning performance to commercial laundry detergents, commercial Persil detergents and water.

TABLE 12 percentage of cleaning compositions, commercial laundry detergents with 2% rice bran enzyme crude extract added, commercial Persil laundry detergents and water for self-made soiled cloths of white cotton-flax mixed cloths, comparative example C, example C-1, example C-2 and example C-3

Test example 5

Test example 5 in order to test the washing abilities of the cleaning compositions containing rice bran enzyme crude extracts of various concentrations of 1%, 2%, or 3%, etc., compared to the commercially available Persil laundry detergent and the commercially available Persil laundry detergent to which the 3% rice bran enzyme crude extract is added, the cleaning abilities of the cleaning compositions of comparative example C, example C-2, example C-3, and example C-4, which are laundry detergents, and the commercially available Persil laundry detergent to which the 3% rice bran enzyme crude extract is added, to white cotton mixed fabrics (self-made soiled fabrics) stained with stains such as fanciful pens or inks, etc., were measured using numerical analysis of stain washing effects, and the commercially available Persil laundry detergent or the commercially available Persil laundry detergent was used as a positive control group, and the clean water washing was used as a negative control group. In the washing method of this example, each soiled cloth was immersed in each cleaning composition, commercially available kokura detergent added with 3% crude extract of rice bran enzyme, commercially available Persil detergent, or clean water, and was washed in a vertical stain removal tester. As shown in fig. 10 and table 13, the results of comparison of groups 47 to 50 show that 1%, 2% and 3% crude extracts of rice bran enzyme significantly enhance the cleaning ability of the cleaning composition of comparative example C for homemade soiled cloths, especially the cleaning ability of the soil of example C-4 containing 3% crude extracts of rice bran enzyme. In addition, the results of comparing groups 51 and 52 show that the 3% rice bran enzyme crude extract also enhances the cleaning ability of commercial laundry detergent for homemade soiled cloths. Furthermore, comparison of the results of groups 48 to 54 shows that the cleaning compositions of examples C-2, C-3 and C-4 have superior cleaning performance to commercial laundry detergents, commercial Persil detergents and water.

TABLE 13 percentage of cleaning compositions, commercial laundry detergents with 3% rice bran enzyme crude extract added, commercial Persil laundry detergents and water for self-made soiled cloths of white cotton-linen blended fabrics compared with comparative example C, example C-2, example C-3 and example C-4

The results of test examples 4 and 5 prove that the cleaning composition containing the crude rice bran enzyme extract of the present invention has a washing performance as a laundry detergent significantly better than that of the commercially available Korea laundry detergent and Persil laundry detergent added with 5 enzymes, and the crude rice bran enzyme extract improves the washing performance of the commercially available Korea laundry detergent, indicating that the crude rice bran enzyme extract of the cleaning composition of the present invention provides excellent laundry cleaning performance.

Test example 6 stability of the cleaning composition

Test example 6-1: to test the effect of the stabilizer formulation on the stability of the crude rice bran enzyme extract in the collar extract formulation of the cleaning composition, 5 g of the crude rice bran enzyme extract powder was added with 3 ml of clear water and left to stand to obtain a clarified solution of the crude rice bran enzyme extract, 1 ml of the clarified solution of the crude rice bran enzyme extract was added to the cleaning composition of comparative example B to a total volume of 10 ml, and left to stand at 4 ℃ (control) and 45 ℃ for one, four and six weeks, and then the enzyme activity of the crude rice bran enzyme extract was measured. As shown in table 14 and fig. 11, the enzyme activities of comparative cleaning composition example B with formulation D1 left at 45 ℃ for one to six weeks were similar to the enzyme activities of comparative cleaning composition example B with formulation D1 left at 4 ℃ for one to six weeks, and the enzyme activities of comparative cleaning composition example B with formulation D1 left at 4 ℃ and 45 ℃ for six weeks were not much different from each other, according to comparative groups 55 to 60. However, delamination occurred after mixing comparative cleaning composition B with stabilizer formulation D1 at 4 ℃ or 45 ℃ for one week (groups 55 and 58) (results not shown). The results of this example demonstrate that stabilizer formulation D1 has the enzyme activity of stabilizing the cleaning composition of comparative example B containing crude rice bran enzyme extract, but it is not possible to avoid delamination.

Table 14 compares the effect of stabilizer formulations on enzyme activity stability of crude rice bran enzyme extract in collar extract formulations

Test example 6-2: to test the effect of different stabilizer formulations on the stability of crude rice bran enzyme extracts in cleaning compositions, the enzyme activity of the crude rice bran enzyme extracts was measured after standing at 4 ℃ (control) and 45 ℃ for one week. The method for measuring the enzymatic activity of the crude rice bran enzyme extract was the same as that of test example 6-1, and the results are shown in table 15, except that the enzymatic activity of the crude rice bran enzyme extract was slightly decreased after the cleaning compositions of groups 62 and 63 were allowed to stand at 45 ℃ for one week, except that the enzymatic activity of the crude rice bran enzyme extract was slightly decreased after group 61 (comparative cleaning composition B mixed with stabilizer formulation D1 prepared in preparation example 3) was allowed to stand at 45 ℃ for one week, compared to the control group allowed to stand at 4 ℃. Furthermore, as shown in fig. 12, both comparative cleaning composition B of groups 61 and 63 mixed with stabilizer formulation D1 or D3 and left standing at 45 ℃ for one week resulted in delamination, while comparative cleaning composition B of group 62 mixed with stabilizer formulation D2 and left standing at 45 ℃ for one week did not show delamination. The results of this example demonstrate that stabilizer formulation D2 has enzyme activity that stabilizes the cleaning composition of comparative example B containing crude rice bran enzyme extract and avoids delamination.

Table 15 compares the effect of different stabilizer formulations on enzyme activity stability of crude rice bran enzyme extract

Test example 7 cleaning efficacy of cleaning compositions containing stabilizer formulations

In order to test the detergency of the cleaning compositions to which the stabilizer formulations were added, the cleaning compositions used in comparative example E and example E-1 of collar extracts were tested for the detergency of self-soiled cloths of white ramie mixed fabrics stained with stains such as fantastic pens, lipstick, or ink as shown in table 16, using numerical analysis of stain-cleaning effect. The cleaning method of this embodiment is to spray the cleaning composition, maobao ferment collar essence or clean water on the stains and to stand for 5 minutes or 20 minutes, then place the stains in a vertical decontamination tester, and clean the stains with clean water or add laundry essence for cleaning.

TABLE 16 cleaning compositions of comparative example E and example E-1 with the addition of stabilizer formulations

The results of the cleaning compositions, the pilocarpine enzyme collar extracts, or the clear water of comparative example E and example E-1 sprayed on the stains and left to stand for 5 minutes, and then washed with water are shown in fig. 13 and table 17; the results of the cleaning compositions, the wool enzyme collar extracts, or the clear water of comparative example E and example E-1, which were sprayed on stains and left standing for 5 minutes, and then washed with the liquid laundry detergent are shown in FIG. 14 and Table 18. The results of comparison of groups 64 to 67 or groups 68 to 71 show that the cleaning composition of example E-1 has significantly better cleaning ability for homemade soiled cloths than the cleaning composition of comparative example E, Maobao enzyme collar essence and clear water, and that the cleaning effect of the laundry detergent is better than that of the water.

Table 17 compares the percentage of laundry detergent after 5 minutes of treatment with the detergent compositions of comparative example E and example E-1, commercially available Maobao enzyme collar essence, and water, and then with water

Table 18 compares the percent of clean-up of homemade soiled cloths after 5 minutes treatment with the cleaning compositions of comparative example E and example E-1, commercially available Maobao enzyme collar cleaner and water and then washed with Korea laundry cleaner

In addition, the results of the cleaning compositions, the pilocarpine enzyme collar extracts, or the clear water of comparative example E and example E-1 sprayed on stains and left to stand for 20 minutes, and then washed with water are shown in fig. 15 and table 19; the results of the cleaning compositions, the pilocarpine enzyme collar extracts, or the clear water of comparative example E and example E-1, which were sprayed on stains and left stand for 20 minutes, and then washed with the laundry detergent are shown in fig. 16 and table 20. The results of comparison of groups 72 to 75 or groups 76 to 79 show that the cleaning composition of example E-1 has significantly better cleaning ability for homemade soiled cloths than the cleaning composition, wool treasure enzyme collar essence and clear water of comparative example E, and that the cleaning effect of the washable laundry essence is better than that of the water.

Table 19 compares the cleaning percentage of the self-made soiled cloths after 20 minutes of treatment with the cleaning compositions of comparative example E and example E-1, the commercially available Maobao enzyme collar essence and water and then washing with water

Table 20 compares the percent of clean-up of homemade soiled cloths washed with laundry detergent after 20 minutes of treatment with the cleaning compositions of comparative example E and example E-1, commercially available Maobao enzyme collar cleaner, and water

Furthermore, by comparing group 69 of Table 18 with group 77 of Table 20, the results show that the cleaning composition of example E-1, after 20 minutes of treatment, had better cleaning ability than the cleaning composition of example E-1, after 5 minutes of treatment.

Test example 8

Test example 8 shows the effect of different enzyme stabilizer formulations on lipase activity, and the results are shown in table 21 and fig. 17.

TABLE 21

1 week	Lipo	Lipex	Lipo+MDA	Lipex+MDA	RBL	RBL+MDA	RBL+Lipo
								F-0	0.014	0.014	0.014	0.0147	0.017	0.0153	0.022
F-1	0.018	0.016	0.016	0.015	0.0164	0.016	0.049
								F-2	0.017	0.015	0.015	0.015	0.0156	0.0165	0.033
F-3	0.0182	0.0175	0.0170	0.016	0.015	0.016	0.030
								F-4	0.018	0.016	0.017	0.015	0.016	0.016	0.035
13 weeks	Lipo	Lipex	Lipo+MDA	Lipex+MDA	RBL	RBL+MDA	RBL+Lipo
								F-0	0.018	0.018	0.017	0.020	0.0273	0.0269	0.033
F-1	0.021	0.021	0.020	0.021	0.028	0.033	0.037
								F-2	0.024	0.020	0.018	0.022	0.026	0.032	0.039
F-3	0.028	0.020	0.019	0.023	0.025	0.033	0.040
								F-4	0.022	0.020	0.022	0.023	0.027	0.029	0.044

Table 21 and fig. 17 show lipase activities after the collar extract formulation F was combined with different enzyme stabilizer formulations and different enzymes were added and left at 45 ℃ for 1 week and 13 weeks.

Detailed description the stabilizer formulations in table 21 are as follows:

detailed description the ferments used in table 21 are as follows:

lipo Novozymes Lipase 100L 1%

Lipex Novozymes Lipase Lipex 100L 1%

MDA (Medium driven multiple Access) 1 percent of novozymesn three-in-one enzyme Medley advance

RBL is lyophilized brown rice sebum lipase 10%.

The codes used in FIG. 17 are detailed below:

f0 example F-0

F1 example F-1

F2 example F-2

F3 example F-3

F4 example F-4

1: standing for 1 week

13, standing for 13 weeks

Lo:Lipolase

Lx:Lipex

LoM:Lipolase+MDA

LxM:Lipex+MDA

R:RBL

RM:RBL+MDA

RL:RBL+Lipolase

From the test results of test example 8, it can be seen that:

1. from the results of the lipase activity measurement in this test example, it was found that RBL + Lipo showed a tendency of higher activity regardless of whether the F collar extract formulation was added or not added with a stabilizer, and RBL alone or RBL + MDA showed a tendency of second highest activity.

2. From the results of the lipase activity measurement in this test example, it was found that the enzyme has the lowest F-0 protective ability and the highest F-1 protective ability when only 2.5% of 1, 2-propanediol is added, and the remaining enzyme stabilizers F-2, F-3 and F-4 have little difference in their activities.

3. From the results of lipase activities at

weeks

1 and 13 in this test example, it was found that the enzyme had little effect on the activity when left at 45 ℃ for 13 weeks.

Test example 9

Test example 9 shows the effect of different enzyme stabilizer formulations on lipase activity, and the results are shown in table 22 and fig. 18.

TABLE 22

Table 22 and fig. 18 show lipase activities after the collar extract formula F was combined with different enzyme stabilizer formulas and different enzymes were added and left at 45 ℃ for 3 weeks, 5 weeks, and 13 weeks. The stabilizer formulations and enzymes used in Table 22 are as described in Table 21. The codes used in fig. 18 are as follows: 3, placing for 3 weeks; 5: standing for 5 weeks, as described in FIG. 17.

From the test results of test example 9, it can be seen that:

1. from the results of the lipase activity measurement in this test example, it was found that RBL + Lipo showed a tendency of higher activity regardless of whether the F collar extract formulation was added or not added with a stabilizer, and RBL alone or RBL + MDA showed a tendency of second higher activity.

2. From the results of the lipase activity measurement in this test example, it was found that F-0 added with 2.5% 1, 2-propanediol alone or F-1 to F-4 enzyme stabilizers did not show much difference in the ability to maintain the activity of the enzyme.

3. From the results of lipase activities at 3, 5 and 13 weeks in this test example, it was found that the enzyme had little effect on the activity when left at 45 ℃ for 13 weeks.

Test example 10

Test example 10 shows the effect of different enzyme stabilizer formulations on lipase activity, and the results are shown in table 23, fig. 19 and fig. 20.

TABLE 23

Table 23, fig. 19 and fig. 20 show lipase activities after the collar extract formula F was combined with different enzyme stabilizer formulas and different enzymes were added and left at 25 ℃ and 45 ℃ for 1 week and 49 weeks. The stabilizer formulations and enzymes used in Table 23 are as described in Table 21. The codes used in fig. 19 and 20 are as follows: 49, standing for 49 weeks, as described in FIG. 17.

From the test results of test example 10, it can be seen that:

1. from the results of the lipase activity measurement in this test example, after heat treatment at 45 ℃ for 1 week or 49 weeks, the formulations (F + RBL, F + RBL + Lipo, F + RBL + MDA) prepared by adding RBL enzyme to F collar extract showed a tendency of higher activity regardless of the addition of a stabilizer, and the formulations added with RBL enzyme were not greatly affected when placed at 45 ℃ for 49 weeks.

2. From the results of the lipase activity measurements in this test example, it was found that RBL and RBL + MDA had no significant effect on the enzyme activity after being left at room temperature (25 ℃) for 1 week and 49 weeks, and that F-0 with only 2.5% 1, 2-propanediol added thereto had the lowest enzyme protective ability and F-4 was the highest, and the remaining enzyme stabilizers F-1, F-2 and F-3 had little difference in enzyme activity maintaining ability, with or without addition of the stabilizer. The lipase activity of RBL + Lipo is higher than that of RBL, Lipo or Lipo + MDA alone.

3. From the lipase activity results of 1 week and 49 weeks in this test example, it was found that RBL or RBL + MDA had little effect on the enzyme activity when placed at 25 ℃ or 45 ℃ for 49 weeks, while RBL + Lipo had 68-82% of the activity at 45 ℃ for 49 weeks, and had no effect only in the F-3 stabilizer formulation.

The above examples are merely illustrative of the methods of making the present invention and are not intended to be limiting. Modifications and variations can be made to the above-described embodiments by those skilled in the art without departing from the spirit and scope of the present invention. Therefore, the scope of the invention should be measured by the appended claims.

Claims

1. A cleaning composition comprising a rice bran enzyme crude extract, a surfactant and a preservative, wherein the rice bran enzyme crude extract is prepared by the following steps:

mixing the rice bran with the enzyme extraction solution in a ratio of 1:8 to 1:2, stirring and centrifuging to obtain rice bran lipase enzyme extract;

adding 20 wt% to 50 wt% of alcohol water solution into the rice bran lipase enzyme solution, stirring and standing, centrifuging or performing gravity settling to obtain the rice bran enzyme crude extract,

2. The cleaning composition of claim 1, wherein the amount of Triton X-100 is in the range of 0.01 wt% to 1 wt% based on the total weight of the enzyme extract solution.

3. The cleaning composition of claim 1, wherein the rice bran ferment crude extract is present in an amount ranging from 0.5 wt% to 15 wt%, based on the total weight of the cleaning composition.

4. The cleaning composition of claim 1, wherein the rice bran ferment crude extract is present in an amount ranging from 5 wt% to 10 wt% based on the total weight of the cleaning composition.

5. The cleaning composition of claim 1, wherein the rice bran ferment crude extract is present in an amount ranging from 0.5 wt% to 2 wt% based on the total weight of the cleaning composition.

6. The cleaning composition of claim 1, wherein the surfactant is selected from the group consisting of sodium dodecyl sulfate, sodium dodecyl benzene sulfonate, sodium dodecyl polyoxyethylene ether sulfate, sodium alpha-olefin sulfonate, methyl polyglycol ether fatty acid ester, lauryl amine oxide, and fatty alcohol polyoxyethylene ether.

7. The cleaning composition of claim 6, wherein the surfactant is present in an amount ranging from 4 wt.% to 15 wt.%, based on the total weight of the cleaning composition.

8. The cleansing composition of claim 1 wherein the preservative is selected from the group consisting of methylparaben, phenoxyethanol, benzoic acid, and sodium benzoate.

9. The cleaning composition of claim 8, wherein the preservative is present in an amount ranging from 0.2 wt.% to 0.6 wt.%, based on the total weight of the cleaning composition.

10. The cleaning composition of claim 1, wherein the cleaning composition further comprises a stabilizer.

11. The cleaning composition of claim 10, wherein the stabilizing agent comprises triethanolamine, sorbitol, 1, 2-propanediol, citric acid, sodium citrate, calcium chloride dihydrate, glycine, and glycerin.

12. The cleaning composition of claim 11, wherein the 1, 2-propanediol is present in an amount ranging from 1.5 wt.% to 6 wt.%, the citric acid is present in an amount ranging from 0.01 wt.% to 6 wt.%, the sodium citrate is present in an amount ranging from 0.1 wt.% to 6 wt.%, the calcium chloride dihydrate is present in an amount ranging from 0.05 wt.% to 0.5 wt.%, the glycine is present in an amount ranging from 0.5 wt.% to 4 wt.%, and the glycerin is present in an amount ranging from 1.5 wt.% to 6 wt.%, based on the total weight of the cleaning composition.

13. A method of cleaning comprising the step of contacting the cleaning composition of any of claims 1 to 12 with laundry to be cleaned.