WO2023056892A1 - Technical stains comprising dna - Google Patents

Technical stains comprising dna Download PDF

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Publication number
WO2023056892A1
WO2023056892A1 PCT/CN2022/123105 CN2022123105W WO2023056892A1 WO 2023056892 A1 WO2023056892 A1 WO 2023056892A1 CN 2022123105 W CN2022123105 W CN 2022123105W WO 2023056892 A1 WO2023056892 A1 WO 2023056892A1
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WO
WIPO (PCT)
Prior art keywords
stain
odor
test material
dna
yellow
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PCT/CN2022/123105
Other languages
French (fr)
Inventor
Qi Wang
Ting Yang
Klaus GORI
Rubini KANNANGARA
Simone Anika Skou OLSEN
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Novozymes A/S
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Publication of WO2023056892A1 publication Critical patent/WO2023056892A1/en

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90Enzymes; Proenzymes
    • G01N2333/914Hydrolases (3)
    • G01N2333/916Hydrolases (3) acting on ester bonds (3.1), e.g. phosphatases (3.1.3), phospholipases C or phospholipases D (3.1.4)
    • G01N2333/922Ribonucleases (RNAses); Deoxyribonucleases (DNAses)

Definitions

  • the present invention relates to a technical stain test material, e.g. in the form of a swatch, comprising a substrate comprising an odor stain and/or a colorant stain comprising DNA.
  • nucleases e.g. deoxyribonucleases
  • WO 2014/087011 WO 2015/155350, WO 2015/155351 and WO 2015/181286, which e.g. discuss the role of DNA in microbial biofilm in textiles.
  • detergent enzymes such as nucleases aimed at removing malodor from e.g. textiles, it is necessary to have access to odor stains on which such enzymes may be tested. This is challenging however, since uniform odor stains that are able to provide a realistic assessment of the malodor removal effect of an enzyme are not readily available.
  • the present invention provides technical stains suitable for testing the malodor and/or yellowing removing effect of enzymes and cleaning compositions, as well as methods for making the stains and use thereof.
  • the stains contain a mixture of compounds that simulate body grime such as sebum as well as a mixture of laundry-relevant malodor compounds and/or a yellow colorant.
  • These technical stains can be used to demonstrate the malodor and/or yellowing removal effect of a detergent containing an enzyme, for example a nuclease enzyme such as a DNase, where malodor can e.g. be analyzed by gas chromatography-mass spectrometry (GC-MS) and/or using human sensory evaluation, while yellow discoloration can e.g. be analyzed by determining remission values using a spectrophotometer.
  • GC-MS gas chromatography-mass spectrometry
  • the present invention relates to stain test materials, e.g. in the form of a swatch, comprising a substrate comprising an odor stain and/or a yellow stain, wherein the odor stain comprises DNA and two or more odor compounds, and optionally synthetic sebum, and wherein the yellow stain comprises DNA, synthetic sebum and at least colorant.
  • the invention further relates to a method for preparing the stain test materials, use of the stain test materials for testing the malodor and/or yellowing removal effect of a cleaning composition comprising an enzyme such as a nuclease, and methods for testing the malodor and/or yellowing removal effect of a cleaning composition comprising an enzyme such as a nuclease.
  • DNA refers to deoxyribonucleic acid from any suitable source, including but not limited to microbial DNA, e.g. bacterial or fungal DNA, or animal DNA, e.g. from a mammal or other vertebrate, for example salmon testes DNA, salmon sperm DNA, herring sperm DNA, calf thymus DNA, DNA from pork or BALB/c mouse DNA.
  • microbial DNA this may be in the form of extracellular DNA (eDNA) e.g. as described in Example 2 herein, or isolated bacterial or fungal DNA, for example DNA from Micrococcus luteus or from Escherichia coli.
  • a ” substrate refers to a textile material which has been or may be treated with an odor stain to result in an odor stain test material.
  • the substrate may be of any suitable textile material such as those listed further below.
  • the substrate is typically a fabric, e.g. comprising one or more materials selected from cotton, polyester, polyacrylic, silk, wool and/or nylon, and/or other materials commonly used for fabrics.
  • the substrate is a cotton, polyester or a cotton/polyester blend fabric.
  • the substrate for use in the present invention will typically be provided in the form of swatches, and the terms “substrate” and “swatch” may be used interchangeably herein.
  • odor stain test material which may also be referred to as an “odor stain swatch” , refers to a test material comprising a substrate and an odor stain, where the odor stain comprises DNA and two or more odor compounds as described herein.
  • odor compound refers to a malodor compound with relevance for common malodors detected in laundry after wear and which may also be detected after wash.
  • the odor compounds should have an odor (malodor) that people would generally consider to be unpleasant, they should be safe to smell so that they can be used for human sensory testing, and they should not be easily washed off by commonly used, commercially available laundry detergents.
  • malodor removal and “malodor removal effect” refer to the ability of an enzyme such as a nuclease, in particular in the form of a detergent composition comprising a nuclease, to remove malodor from a material or reduce the amount of malodor in the material.
  • the word “removal” in the context of the term “malodor removal” includes “reduction” and should thus be understood to include any measurable reduction of malodor.
  • yellowing removal and “yellowing removal effect” refer to the ability of an enzyme such as a nuclease, in particular in the form of a detergent composition comprising a nuclease, to remove yellowing from a material or reduce the amount of yellowing in the material.
  • the word “removal” in the context of the term “yellowing removal” includes “reduction” and should thus be understood to include any measurable reduction of yellowing.
  • nuclease refers to any nuclease enzyme, including enzymes with deoxyribonuclease activity (DNases) or ribonuclease activity (RNases) as well as enzymes that have both DNase and RNase activity.
  • DNases deoxyribonuclease activity
  • RNases ribonuclease activity
  • the odor stain test materials of the invention may be used to test the malodor removing effect of any desired nuclease.
  • DNase refers to a polypeptide with DNase activity that catalyzes the hydrolytic cleavage of phosphodiester linkages in the DNA backbone, thus degrading DNA.
  • synthetic sebum refers to a standardized synthetic sebum product that is designed to emulate natural human sebum.
  • a number of different synthetic sebum products are commercially available, for example from Center for Testmaterials (CFT) (the Netherlands) and from wfk-Testgewebe GmbH (Germany) , as well as from other suppliers, and any such synthetic sebum product may be used in the context of the present invention.
  • Synthetic sebum products typically comprise a mixture of components such as free fatty acids, fatty acid triglycerides, cholesterol, vegetable oil such as olive oil, tallow such as beef tallow, and hydrocarbons.
  • the term “fabric” refers to textile materials including non-woven materials, natural materials, synthetic materials, and any other textile material, fabrics made of these materials and products made from fabrics (e.g., garments and other articles) .
  • the textile or fabric may be in the form of knits, wovens, denims, non-wovens, felts, yarns or toweling.
  • the textile may be cellulose-based such as natural cellulosics, including cotton, flax/linen, jute, ramie, sisal or coir, or manmade cellulosics (e.g.
  • the textile or fabric may also be non-cellulose based such as natural polyamides including wool, camel, cashmere, mohair, rabbit or silk or synthetic polymers such as nylon, aramid, polyester, acrylic, polypropylene or spandex/elastane, or blends thereof as well as blends of cellulose based and non-cellulose based fibers.
  • non-cellulose based such as natural polyamides including wool, camel, cashmere, mohair, rabbit or silk or synthetic polymers such as nylon, aramid, polyester, acrylic, polypropylene or spandex/elastane, or blends thereof as well as blends of cellulose based and non-cellulose based fibers.
  • blends are blends of cotton and/or rayon/viscose with one or more companion material such as wool, synthetic fiber (e.g.
  • polyamide fiber acrylic fiber, polyester fiber, polyvinyl chloride fiber, polyurethane fiber, polyurea fiber, aramid fiber
  • polyester/cotton such as polyester/cotton
  • cellulose-containing fiber e.g. rayon/viscose, ramie, flax/linen, jute, cellulose acetate fiber, lyocell
  • the terms “textile” and “fabric” may be used interchangeably.
  • detergent composition refers to a composition that finds use in the removal of undesired compounds from items to be cleaned, for example textiles.
  • Detergent compositions may be used to clean e.g. textiles for both household cleaning and industrial cleaning.
  • the term encompasses any materials/compounds selected for the particular type of cleaning composition desired and the form of the product (e.g., liquid, gel, powder, granulate, paste, or spray compositions) and includes e.g., liquid and/or solid laundry detergents and fine fabric detergents; fabric fresheners; fabric softeners; and textile and laundry pre-spotters/pretreatment.
  • detergent compositions may contain one or more additional enzymes (such as proteases, amylases, lipases, cutinases, cellulases, endoglucanases, xyloglucanases, pectinases, pectin lyases, xanthanases, peroxidases, haloperoxygenases, catalases and mannanases, or any mixture thereof) , and will typically contain one or more detergent adjunct ingredients such as surfactants, builders, chelators or chelating agents, bleach system or bleach components, polymers, fabric conditioners, foam boosters, suds suppressors, dyes, perfume, tannish inhibitors, optical brighteners, bactericides, fungicides, soil suspending agents, anti-corrosion agents, enzyme inhibitors or stabilizers, enzyme activators, oxido-reductases, bluing agents and fluorescent dyes, antioxidants and/or solubilizers.
  • additional enzymes such as proteases, amylases,
  • laundering includes both household laundering and industrial laundering and means the process of treating textiles with a solution containing a cleaning or detergent composition.
  • the laundering process can for example be carried out using e.g. a household or an industrial washing machine or can be carried out by hand.
  • yellow discoloration refers to discoloration of textiles, in particular clothing items that are in contact with the body, and especially areas such as collars, underarms, cuffs etc. that are subject to sebum and sweat, and where microbial biofilm that can contribute to yellow discoloration tends to form.
  • yellow stain test material refers to a test material comprising a substrate and a yellow stain, where the yellow stain comprises DNA, synthetic sebum and at least one yellow colorant as described herein.
  • yellow colorant refers to any suitable colorant with a yellowish or orange hue.
  • the colorant will typically be a microbial, e.g. bacterial, colorant, typically a carotenoid such as lutein.
  • Other suitable colorants include flexirubin, ankaflavin, monaphilone A, zeaxanthin, astaxanthin, ⁇ -carotene, riboflavin and azaphilones (see e.g. Aruldass et al., Journal of Cleaner Production 180 (2016) 168e182) .
  • references to “about” a value or parameter herein includes aspects that are directed to that value or parameter per se.
  • a reference to “about X” includes the aspect “X” .
  • the invention provides technical stains in the form of a test material, wherein the test material comprises a substrate comprising a) DNA, b) two or more odor compounds and/or at least one yellow colorant, and optionally c) synthetic sebum.
  • the stains may be an odor stain, a yellow stain, or a combined odor and yellow stain.
  • the present invention relates to an odor stain test material, e.g. in the form of a swatch, comprising a substrate comprising an odor stain, wherein the odor stain comprises DNA, optionally synthetic sebum, and two or more odor compounds.
  • the invention in another aspect, relates to a yellow stain test material, e.g. in the form of a swatch, comprising a substrate comprising a yellow stain, wherein the yellow stain comprises DNA, synthetic sebum and at least one yellow colorant.
  • the invention relates to a stain test material, e.g. in the form of a swatch, comprising a substrate comprising a combined odor stain and yellow stain, wherein the substrate comprises DNA, synthetic sebum, two or more odor compounds, and a least one yellow colorant.
  • the substrate of the stain test material of the invention is typically a fabric, for example comprising cotton, polyester, polyacrylic, silk, wool and/or nylon.
  • the substrate comprises cotton, polyester or a cotton/polyester blend fabric, since these are commonly used materials in clothing, towels, bedding, etc.
  • the substrate may comprise, consist essentially of or consist of synthetic polymers such as nylon, aramid, polyester, acrylic, polypropylene and spandex/elastane, or blends thereof as well as blends of one or more of these materials with cellulose based and/or non-cellulose based fibers.
  • Synthetic materials are in some cases of particular interest for testing the malodor removing effect of a nuclease, since malodor compounds often tend to be more difficult to remove from hydrophobic synthetic materials such as polyester than from materials such as cotton, e.g. in the case of odors that are difficult to remove from athletic clothing made of synthetic materials.
  • Preferred substrate materials include cotton, polyester and cotton/polyester blends.
  • the fabric may e.g. contain the two materials in a ratio of from 95: 5 to 5: 95, preferably from 80: 20 to 20: 80, such as from 65: 35 to 35: 65, e.g. about 50: 50.
  • the substrate or swatch is aged. Aging of a test swatch is used to mimic the situation where fabrics are worn or used rather than being new. Aging of a swatch can e.g. be done with the Warscator machine (Electrolux Professional FOM71CLS) by using Program 152 with tap water at 40°C, where the aging time can range from 1 hr to 24 hrs, preferably from 5 hrs to 20 hrs, more preferably from 8 hrs to 14 hrs.
  • the DNA may be from any suitable source, e.g. microbial DNA or animal DNA.
  • animal DNA it is apparent that this will be in the form of isolated DNA that is applied to the substrate.
  • microbial DNA this may be either in the form of extracellular DNA (eDNA) obtained e.g. as described in Example 2 herein, where a swatch (i.e. the substrate) is incubated in the presence of a microorganism that produces eDNA, or it may be applied to the substrate in the form of isolated bacterial or fungal DNA.
  • eDNA extracellular DNA
  • the amount of DNA for an individual swatch will depend on factors such as the size of the swatch and the type of DNA, and will be able to be determined by persons skilled in the art. It will be apparent that when isolated DNA is applied to a swatch, the amount of DNA will vary depending e.g. on the size of the swatch. As an example, for a 5 cm x 5 cm textile swatch with isolated DNA such as salmon testes DNA applied, a suitable amount of DNA may e.g. be in the range of about 100-1000 ⁇ l of 1%DNA, for example about 200-600 ⁇ l of 1%DNA, such as about 300-500 ⁇ l of 1%DNA.
  • a suitable amount of microbial eDNA may e.g. be provided using 1-2 ml of a starting culture of the microorganism of interest, allowing the swatches to grow in the culture under suitable conditions of temperature etc. for a suitable period of time, for example about 24 hours; see Example 2 herein. It should be emphasized that these are non-limiting examples only, as it will be apparent that the DNA, whether isolated DNA or microbial eDNA, may be provided by various means and in various amounts.
  • the odor stain comprises synthetic sebum.
  • the synthetic sebum is used in combination with isolated DNA which is applied to the substrate as a mixture containing the sebum and the DNA, and preferably an emulsifier, for example as described in Example 1.
  • isolated DNA which is applied to the substrate as a mixture containing the sebum and the DNA, and preferably an emulsifier, for example as described in Example 1.
  • Malodor compounds bound to a mixture of sebum and DNA can be particularly difficult to remove from fabrics, thus the use of a combination of synthetic sebum and DNA allows identification of enzymes, for example nucleases such as DNases, that may be expected to be effective at removing malodor compounds in challenging situations.
  • synthetic sebum products are commercially available, and the amount of sebum to be applied can therefore readily be determined by persons skilled in the art.
  • the DNA is microbial eDNA, in which case the odor stain test material may comprise synthetic sebum or it may be without synthetic sebum.
  • Microbial eDNA contains dead bacterial cells as well as proteins and other cellular debris which contribute to malodor binding even in the absence of sebum.
  • a substrate containing microbial eDNA can therefore perform similarly to a substrate containing isolated DNA and sebum even without the addition of synthetic sebum.
  • volatile odor compounds also referred to herein as “malodor compounds”
  • laundry-relevant volatile odor compounds as long as they have an odor that most people would consider to be unpleasant, are safe to smell, and are not too easily removed from the relevant substrate material by common laundry detergents.
  • Volatile odor compounds are compounds that are sufficiently volatile to be transmitted via the air to the olfactory system and bind to receptors in the nose, thus being perceived as a smell or odor. While compounds such as perfume compounds are perceived as having a pleasant smell, malodor compounds are perceived as having an unpleasant smell. Such volatile compounds generally have a molecular weight of less than about 300 g/mol, typically less than about 200 g/mol.
  • Suitable odor compounds may include compounds from one or more categories selected from fatty acids, steroid compounds, sulfur compounds, ketones, esters, aldehydes and alcohols.
  • the odor compounds include at least one aldehyde, preferably two or more aldehydes.
  • Suitable odor compounds include those from the groups listed below:
  • Fatty acids for example acetic acid, propanoic acid, 2-methylpropanoic acid (isobutyric acid) , butanoic acid, 2-methylbutanoic acid, 3-methylbutanoic acid (isovaleric acid) , 3-methyl-2-hexenoic acid, 4-methyl-3-hexenoic acid (4M3H) , 5-methyl-4-hexenoic acid, 3-methyl-3-hydroxy-hexanoic acid, 6-heptenoic acid, 4-methyloctanoic acid, 4-ethyloctanoic acid, (2E) -3-methylhex-2-enoic acid.
  • acetic acid for example acetic acid, propanoic acid, 2-methylpropanoic acid (isobutyric acid) , butanoic acid, 2-methylbutanoic acid, 3-methylbutanoic acid (isovaleric acid) , 3-methyl-2-hexenoic acid, 4-methyl-3-hexenoic acid (4M3H) ,
  • Steroid compounds for example 5- ⁇ -androstenol, 5- ⁇ -andro-stenone, 5- ⁇ -androst-2-en-17-one, 5- ⁇ -androst-16-ene-3-one.
  • Sulfur compounds for example 3-methyl-3-sulfanyl-hexan-1-ol (3M3SH) , dimethyl disulphides, dimethyl trisulphides, benzyl mercaptan.
  • Ketones for example 1-hexen-3-one, 2-heptanone, 2-octanone, 1-octen-3-one, 2-nonanone, (E) -3-octen-2-one, other medium chain ketones.
  • Esters for example ethyl-2-methylpropanoate, ethyl butanoate, methyl-3-methyl hexanoate, methyl laurate, methyl myristate, 2-aminoacetophenone, diethyl phthalate.
  • Aldehydes for example methional, pentanal, heptanal, hexanal, nonanal, (Z) -4-heptnal, octanal, (E) -2-octenal, cis/trans-2-nonenal, (E, Z) -2, 4-nonadienal, (E, Z) -2, 6-nonadienal, decanal, (E) -2-decenal, (E, E) -2, 4-decadienal, (E) -4, 5-epoxy-E-2-decenal, 4-methoxybenzaldehyde, 4-isopropylbenzaldehyde, cuminaldehyde, other medium-chain aldehydes.
  • Alcohols for example oct-1-en-3-ol, 2-nonanol, 1-decanol, 1-dodecanol, 2-phenylethanol, 2-methoxyphenol (guaiacol) .
  • Examples of some preferred odor compounds for use in the present invention include the following:
  • the odor compounds may be diluted in any appropriate solvent, for example dipropylene glycol as described in the examples below.
  • the amount of odor compounds to be used may be readily determined by persons skilled in the art based e.g. on the nature of the substrate, the size of the swatch, and the type and amount of DNA, to result in a swatch that allows suitable differentiation of residual odor compounds after the test materials are washed using the chosen nuclease, detergent and wash conditions.
  • a suitable amount of malodor compounds may e.g. be approximately as described in the examples, for example about 80 ⁇ l of a malodor mix such as that described in Example 1.
  • an example of suitable amount of malodor compounds may e.g. be about 10 ⁇ l of a malodor mix such as that described in Example 2. It should be emphasized that these are non-limiting examples only, as it will be apparent that the malodor compounds may be applied in different amounts and concentrations depending on factors such as the nature of the substrate, the size of the swatch, the amount and type of DNA and optionally sebum, and the number of different malodor compounds used.
  • the odor stain test material of the invention comprises at least two odor compounds and will typically comprise more than two such compounds, e.g. three, four, five, six, seven, eight, nine, ten or more odor compounds, for example selected from those groups of compounds and individual compounds listed above.
  • the odor stain comprises two or more odor compounds selected from the group consisting of pentanal, hexanal, heptanal, (Z) -4-heptenal, octanal, 1-octen-3-one, nonanal, (E) -3-octen-2-one, trans-2-nonenal, (E) -2-decenal, (E, E) -2, 4-decadienal, decanal, 4-isopropylbenzaldehyde, isovaleric acid, (2E) -3-methylhex-2-enoic acid, 4-methyloctanoic acid and cuminaldehyde.
  • odor compounds selected from the group consisting of pentanal, hexanal, heptanal, (Z) -4-heptenal, octanal, 1-octen-3-one, nonanal, (E) -3-octen-2-one, trans-2-nonenal, (E) -2
  • the odor stain may comprise three or more such compounds, e.g. four, five, six, seven, eight or more odor compounds selected from the group consisting of pentanal, hexanal, heptanal, (Z) -4-heptenal, octanal, 1-octen-3-one, nonanal, (E) -3-octen-2-one, trans-2-nonenal, (E) -2-decenal, (E, E) -2, 4-decadienal, decanal, 4-isopropyl-benzaldehyde, isovaleric acid, (2E) -3-methylhex-2-enoic acid, 4-methyloctanoic acid and cuminaldehyde.
  • odor compounds selected from the group consisting of pentanal, hexanal, heptanal, (Z) -4-heptenal, octanal, 1-octen-3-one, nonanal, (E) -3
  • the odor stain test material may further comprise an emulsifier, in particular where the odor stain includes synthetic sebum.
  • Suitable emulsifiers include those that can help to form oil-in-water (O/W) emulsions, preferably emulsifiers with a Hydrophilic-Lipophilic Balance (HLB) value in the range of between about 8 and 16.
  • the emulsifier may, e.g., be a polysorbate emulsifier having a suitable HLB value, for example polysorbate 80 (polyoxyethylene sorbitan monooleate, for example 80) .
  • the invention relates to a method for preparing an odor stain test material, the method comprising applying DNA, two or more odor compounds and optionally an emulsifier to a substrate.
  • the substrate and the odor compounds may comprise any of those described above.
  • the method involves applying a mixture of DNA, synthetic sebum and optional emulsifier to the substrate prior to application of the odor compounds.
  • the stain comprises both DNA and artificial sebum as explained above.
  • the DNA and sebum may be applied to the substrate separately, but they are preferably applied as a mixture of DNA and synthetic sebum, typically in the form of an emulsion prepared using any suitable emulsifier as mentioned above.
  • the DNA, or DNA and artificial sebum is typically applied to the substrate and then dried, for example drying at room temperature e.g. overnight, before the odor compounds are applied to the substrate.
  • An example of preparation of odor stain swatches comprising isolated DNA and artificial sebum is provided in Example 1 herein.
  • the DNA can be supplied in the form of powders or liquid solutions and can be formulated as needed.
  • a DNA solution can be prepared by dissolving the DNA in water or another suitable solvent or buffer.
  • a DNA solution may, for example, be provided in the concentration of from 0.1 mg/mL to 20 mg/mL, for example from 5 mg/mL to 15 mg/mL, e.g. from 8 mg/mL to 12 mg/mL.
  • the DNA is eDNA obtained by incubating the substrate (e.g. a fabric swatch) in the presence of a microorganism that produces eDNA under conditions that allow for growth of the microorganism and deposit of eDNA on the substrate.
  • the substrate is then typically rinsed with water and then dried, e.g. at room temperature, before application of odor compounds to the dry substrate.
  • Artificial sebum may optionally be added to the substrate, e.g. before or after rinsing the substrate following microorganism growth, or prior to microorganism growth.
  • a substrate containing microbial eDNA can be without sebum.
  • the invention relates to 1) use of an odor stain test material as described herein for testing the malodor removal effect of a cleaning composition comprising an enzyme such as a nuclease; and 2) a method for testing the malodor removal effect of a cleaning composition comprising an enzyme such as a nuclease, the method comprising providing an odor stain test material as described herein, subjecting the odor stain test material to washing with a cleaning composition comprising a nuclease or other enzyme, and evaluating residual malodor on the odor stain after wash.
  • an odor stain test material which has been prepared as described herein using any of the methods and materials disclosed above or e.g. in the examples below, is washed with a cleaning composition comprising a nuclease, after which the malodor removal effect of the cleaning composition comprising a nuclease is determined by measuring the residual malodor after wash.
  • a yellow stain test material may be prepared substantially as described above for the odor stain test material in relation to the DNA component and the synthetic sebum component. However, instead of or in addition to the two or more odor compounds, the yellow stain test material will comprise at least one yellow colorant.
  • the yellow colorant may be any suitable colorant with a yellowish or orange hue, and will typically be a microbial, e.g. bacterial, colorant, typically a carotenoid, such as lutein.
  • suitable colorants include flexirubin, ankaflavin, monaphilone A, zeaxanthin, astaxanthin, ⁇ -carotene, riboflavin and azaphilones.
  • the yellow colorant is a carotenoid, with a preferred carotenoid being lutein.
  • Carotenoids for example lutein, zeaxanthin and ⁇ -carotene, are hydrophobic molecules.
  • the yellow stain test materials of the invention therefore preferably contain an emulsifier. This allows the hydrophilic DNA to be more readily mixed with the hydrophobic sebum and colorant and applied to a textile.
  • the emulsifier may be as described above in the context of the odor stain test material, for example a polysorbate emulsifier such as polysorbate 80.
  • the invention relates to 1) use of a yellow stain test material as described herein for testing the yellowing removal effect of a cleaning composition comprising an enzyme such as a nuclease; and 2) a method for testing the yellowing removal effect of a cleaning composition comprising an enzyme such as a nuclease, the method comprising providing a yellow stain test material as described herein, subjecting the yellow stain test material to washing with a cleaning composition comprising a nuclease or other enzyme, and evaluating the whiteness of the yellow stain after wash.
  • a yellow stain test material which has been prepared as described herein using any of the methods and materials disclosed above or e.g. in the examples below, is washed with a cleaning composition comprising a nuclease, after which the yellowing removal or reduction effect of the cleaning composition comprising a nuclease is determined by measuring the whiteness of the yellow stain after wash.
  • the stain test material of the invention comprises both an odor stain and a yellow stain in the form of a combined odor and yellow stain.
  • the substrate comprises a combined odor and yellow stain, wherein said stain comprises DNA, two or more odor compounds, at least one yellow colorant, synthetic sebum, and preferably an emulsifier.
  • An example of such a stain test material comprising a combined odor and yellow stain and use thereof is provided in Example 4.
  • the invention relates to a method for preparing a yellow stain test material, the method comprising applying DNA, synthetic sebum, at least one yellow colorant and preferably an emulsifier to a substrate.
  • the substrate and yellow colorant may comprise any of those described above.
  • the method may e.g. be performed by first forming a mixture of DNA, synthetic sebum, yellow colorant and emulsifier, whereafter this mixture is applied to the substrate.
  • the odor compounds may be suitably applied to the substrate after application of the mixture of DNA, synthetic sebum, yellow colorant and emulsifier.
  • divalent ions such as magnesium or calcium ions may be included in the yellow stain, odor stain or combined yellow and odor stain. This serves to reduce the stain’s sensitivity to DNase, which has been found to better mimic the effect of DNase on real laundry items, thus allowing for a more realistic assessment of the ability of different DNases to remove yellow and/or odor stains and a better dose response to addition of DNase.
  • Preferred divalent ions include magnesium ions, e.g. in the form of magnesium chloride (MgCl 2 ) .
  • Calcium ions e.g. in the form of calcium chloride (CaCl 2 ) , may also be used.
  • the divalent ions may be added to the mixture of DNA, synthetic sebum, yellow colorant and preferably an emulsifier.
  • the divalent ions may e.g. be added a mixture of DNA, odor compounds, optional synthetic sebum and optional emulsifier.
  • magnesium ions these may be added to result in a concentration of magnesium chloride in the range of from about 1 mM to about 40 mM, for example from about 2 mM to about 20 mM, such as from about 5 mM to about 15 mM, such as about 10 mM.
  • magnesium ions these may be added to result in a concentration of calcium chloride in the range of from about 1 mM to about 500 mM, such as from about 5 to about 50 mM.
  • Testing the malodor or yellowing removal effect of a cleaning composition comprising a nuclease using an odor stain test material of the invention is not limited to any particular nuclease or cleaning composition. Rather, any nuclease in any desired cleaning/detergent composition may be tested in order to determine the malodor removal effect of a chosen nuclease in a chosen composition.
  • the nucleases that may be tested using the stain test materials and methods of the invention may include DNases, RNases, and enzymes with both DNase and RNase activity.
  • the nuclease is a DNase, including DNase polypeptides derived from bacteria and fungi, as well as genetically engineered variants thereof.
  • DNases include DNases derived from Aspergillus, e.g. Aspergillus oryzae, such as those disclosed in WO 2015/155350, DNases derived from Trichoderma, e.g. Trichoderma harzianum, such as those disclosed in WO 2015/155351, DNases derived from genera formerly classified as Bacillus, e.g.
  • Metabacillus indicus (formerly known as Bacillus cibi) , such as those disclosed in WO 2017/060475, DNases derived from Vibrissea, e.g. Vibrissea flavovirens, such as those disclosed in WO 2017/060493, DNases derived from Morchella, e.g. Morchella costata, such as those disclosed in WO 2018/177203, and DNases derived from Rhizoctonia, e.g. Rhizoctonia solani, such as those disclosed in WO 2018/177938.
  • Vibrissea e.g. Vibrissea flavovirens
  • Morchella e.g. Morchella costata
  • Rhizoctonia e.g. Rhizoctonia solani
  • DNases are also included, for example variants of a DNase from Aspergillus oryzae such as those disclosed in WO 2017/064269, and variants of a DNase from Metabacillus indicus (formerly known as Bacillus cibi) such as those disclosed in WO 2018/011277, WO 2019/081724 and WO 2019/081721.
  • a DNase from Aspergillus oryzae such as those disclosed in WO 2017/064269
  • variants of a DNase from Metabacillus indicus (formerly known as Bacillus cibi) such as those disclosed in WO 2018/011277, WO 2019/081724 and WO 2019/081721.
  • a preferred method for analysis of residual malodor of an odor stain swatch of the invention is by chromatography-mass spectrometry (GC-MS) .
  • GC-MS combines gas chromatography to separate the compounds in a chemical mixture and mass spectrometry to identify the compounds at the molecular level.
  • GC-MS is particularly applicable to analysis of volatile compounds, and is therefore well-suited to analyzing volatile odor compounds that are the cause of malodor on e.g. textiles.
  • GC-MS analysis is a technique which is well-known to persons skilled in the art, and instruments for GC-MS analysis are commercially available.
  • residual malodor may be analyzed by human sensory evaluation, e.g. using persons specially trained to detect odors and/or a panel of consumer test persons.
  • Determination of the yellowing removal or reduction effect may suitably be performed by measuring the remission values using a spectrophotometer, e.g. as described in the examples below.
  • the remission values are correlated with the “whiteness” of the fabric and thus with the degree to which the yellow stain has been removed.
  • Example 1 Stain containing sebum, DNA and malodor to show malodor removal effect in detergents with a DNase
  • Knitted cotton fabric (CN-42, Center For Testmaterials BV, the Netherlands) was prewashed as described below and cut into pieces of 5 cm ⁇ 5 cm.
  • Prewash of textiles was done primarily to remove starch, carboxymethyl cellulose (CMC) and other additives from the textiles.
  • CMC carboxymethyl cellulose
  • Three kg of textile fabrics were washed three times in 78.6 g of W-ECE-2 detergent (wfk Testgewebe GmbH, Germany) using water with 15°dH water hardness [2.139 mM CaCl 2 , 0.5355 mM MgCl 2 , 4.0125 mM NaHCO 3 dissolved in deionized water] and containing the following enzymes (available from Novozymes A/S, Denmark) for each of the three prewash steps :
  • Prewashing was carried out in a Miele Softtronic W1935 WTL machine at 40°C using a standard washing program with 13-15 liters of water for 3 kg of textile. After the third prewash step, a rinse was carried out in deionized water and textile fabrics were then line dried.
  • Deoxyribonucleic acid (DNA) sodium salt from salmon testes (Sigma Aldrich) was dissolved in MilliQ water in a 50 ml falcon tube to prepare a 1%DNA solution by rotating the tubes at 20 rpm for 1 h. While rotating the DNA solution, artificial sebum (BEY sebum, wfk-Testgewebe GmbH, Germany) was melted in a glass beaker in a water bath at 90°C. After DNA was fully dissolved and the sebum was melted, 1.6 ml sebum was transferred into 30 ml 1%DNA in a glass beaker with magnetic stirring.
  • Sebum and DNA solution were mixed by vigorous pipetting to form an emulsion, and 0.02% (v/v) 80 (Sigma Aldrich) was added to the emulsion to stabilize it.
  • the glass beaker was put in a water bath on a heat stirrer with a stirring speed of 400 rpm. To avoid water evaporation during stirring, the glass beaker was covered with tin foil.
  • the Tergotometer consists of sixteen 2 L metal beakers, each fitted with an agitator which rotates in a back-and-forth manner at a controlled speed (120 rpm) to simulate the agitation mode occurring in commercial top-loader washing machines.
  • the beakers were partly submerged in thermostatic water baths where the temperature was kept at 30°C.
  • Four stains were washed in one beaker filled with 1 L detergent solution (15 dH water with 5.1 g/L Ropa Blanca Y Color or with 5.3 g/L Gut &Günstig Aktiv Vollwaschstoff) , and 1.0 ppm DNase was added to each beaker.
  • the DNase was a fungal DNase derived from Aspergillus oryzae (mature polypeptide of SEQ ID NO: 2 as disclosed in WO 2015/155350) . Washes without enzyme were included as controls. Two replicates (1 replicate/beaker) were used for each condition (detergent/DNase combination) .
  • each odor stain was centrifuged in a falcon tube containing a filter (HOZELOCK K3 Kaldness filter, Aquacadabra, eBey) at 4000 g for 5 min to remove excess water. This plastic filter separated the excess water from the swatch after centrifuging.
  • a filter HOZELOCK K3 Kaldness filter, Aquacadabra, eBey
  • ⁇ GC oven temperature initial 40°C; hold 2 min; rate 5°C/min until 180°C; rate 30°C/min until 240°C; hold 0 min.
  • Front SS Inlet He Mode Split; T a 230°C, Split Ratio 10: 1; Split Flow 15 mL/min.
  • Gerstel MPS SPME Incubator Agitator. incubation temperature: 60°C; incubation time: 10.00 min; agitator speed: 250 rpm; sample parameters: extraction time: 2.00 min; inj. desorption time: 120 s.
  • MS Information Acquisition Mode: Scan. Solvent Delay (minutes) : 1. Scan Parameters: Start Time: 1. Low Mass: 35. High Mass: 350. Threshold: 100. A/D Samples: 4. MSZones: MS Source: 230°C. MS Quad: 150°C
  • the DNase also showed good malodor removal benefit in the two powder detergents, Persil Color 360° and Persil Universal Pulver Tiefenrein, with an overall reduction of 38.1%and 52.7%, respectively. It is noteworthy that Persil Universal Pulver Tiefenrein contains 15-30%oxygen-based bleach in its formulation. Thus the results show that the DNase is also effective in reducing malodor in bleach-containing detergents.
  • Example 2 Stain containing microbial extracellular DNA and malodor to show malodor removal effect in detergents with a DNase
  • Knitted cotton fabric (CN-42, Center For Testmaterials BV, the Netherlands) was pre-washed and cut into round pieces with diameter of 2 cm.
  • TSA Tryptone Soya Agar
  • CM0131 Oxoid Lid. Basurgstukc, UK
  • TAB Tryptone Soya Broth
  • Pseudomonas fluorescens was pelleted by centrifugation (Sigma Laboritory Centrifuge 6K15) (3000 g at 21 °C, in 7 min) and resuspended in 10 mL of TSB diluted twice with water.
  • Optical density (OD) at 600 nm was measured using a spectrophometer (POLARstar Omega (BMG Labtech, Ortenberg, Germany) .
  • Fresh TSB diluted twice with water was inoculated to an OD 600nm of 0.03, and 1.6 ml was added into each well of a 12-well polystyrene flat-bottom microplate (3512; Corning Incorporated, Corning, N.
  • the stains were left on a plastic tray in a flow cabinet to dry at room temperature for 24 h. Then the tray was moved to a fume hood and 10 ⁇ l of malodor mix (Table 6) was added to the center of each stain. The odor stain was left in the fume hood for 1 h before washing.
  • wash solutions of liquid model detergent A2 or powder model detergent T (without bleach) were prepared by weighing the detergents and dissolving them in water with 15°dH water. Dosing of model detergent A and T (without bleach) was 3.33 g/L and 5.3 g/L, respectively.
  • the miniLOM assay uses a small-scale version of a (LOM) .
  • a miniLOM basically consists of closed test tubes which are rotated in a heating cabinet for a given time and at a given temperature. Each test tube represents one small washing machine. It is mainly used in testing of detergents and enzymes at European wash conditions.
  • an enzyme blend Brilliant (Novozymes A/S, Denmark) was added to all glass tubes at the dosage of 2.5%of the detergent.
  • the enzyme blend contains a variety of commonly used enzymes, including protease, amylase, lipase, mannanase, pectate lyase and two cellulases.
  • the glass tubes were then mounted in a mini-Launder-Ometer (aStuart Tube Rotator SB3) and washed at 30°C for 60 minutes at 20 rpm. After wash, the rotator was placed at room temperature while swatches from one glass tube at a time were rinsed with 15°dH water and placed back into the rotator. Each glass tube was rinsed twice in 30 mL 15°dH water.
  • Example 3 Stain containing sebum, DNA and yellow colorant to show yellow color removal effect in detergent with a DNase
  • the textile fabrics used were cotton (W-80A) , polyester/cotton (polycotton) (T-7422) , polyester (T720) . All fabrics were purchased from Center For Testmaterials B.V, The Netherlands. The fabrics were prewashed as described above in Example 1 before and cut into pieces of 6 cm ⁇ 6 cm.
  • lutein (CAS Number 127-40-2, Shanghai Aladdin Bio-Chem Technology Co., LTD, China) was added to the mixture to provide yellow color to the stain. The specific procedure is described below.
  • 600 ⁇ L, 600 ⁇ L or 400 ⁇ L of the mix solution was applied to cotton, polycotton or polyester swatches, respectively. Fabrics were picked up one at a time and place on the lid of 50 mL tubes, stretching the fabric with fingers, and the mix solution was smeared evenly using a finger for thirty cycles in a clockwise direction. The swatches were subsequently dried overnight at room temperature without direct sunlight.
  • a commercial EU liquid detergent was used: Domol Colorwaschsch Violet Dream (Nopa Nordic A/S, Denmark) .
  • Washes were performed in water with 15°dH [2.139 mM CaCl 2 , 0.5355 mM MgCl 2 , 4.0125 mM NaHCO 3 dissolved in MilliQ water] in a Terg-O-tometer (TOM) instrument.
  • the TOM instrument consisted of 1 L beakers with agitator spindles inserted into the beakers and rotated in a back-and forth manner at a controlled speed (120rpm) to simulate a commercial top-loader/vertical drum washing machine.
  • the beakers were immersed in a temperature-controlled water bath at 30°C during wash.
  • the detergent was dissolved in 15°dH water at a concentration of 3.5 g/L.
  • the stains were promptly removed from the beakers and rinsed thoroughly under running deionized water for 30 sec. Then they were allowed to air dry on filter paper overnight at room temperature or until dry. The stains were yellow or pale yellow after washing and drying.
  • the dried stains were measured for whiteness on a Datacolor 800V spectrophotometer (Datacolor, Lawrenceville, NJ, USA) under the CIE Standard Illuminant D65 and the CIE 1964 10-degree Standard Observer. The measurements were made without UV in the incident light, and remission values at 460 nm were recorded for textile traces washed with and without DNase. The yellow color removal benefits were evident when the differences in remission between stains washed with and without DNase were higher than two units. For these remission value differences, the human eye is be able to detect the yellow color removal benefits between stains.
  • the yellow stains with DNA of this example were able to show a good yellow color removal benefit of the DNase in the liquid EU detergent for all fabric types, and both with and without magnesium ions, with remission value differences that were easily distinguishable to the human eye.
  • the results for the different fabrics are provided below in Tables 9 and 10, where Table 10 shows the results including 10 mM Mg 2+ and Table 9 shows the results without Mg 2+ .
  • Example 4 Stain containing sebum, DNA, yellow colorant and malodor to show yellow color and malodor removal effect in detergent with a DNase
  • Knitted cotton fabric (CN-42) was pre-washed as described above and cut into pieces of 5 cm ⁇ 5 cm.
  • the stain was prepared as described above in Example 3, with two small differences: 1) the sebum was not pre-aged; 2) the mix of DNA, sebum and yellow color was not applied to the fabric by finger, but by pipetting to the center of the swatches as in Examples 1 and 2. The specific procedure is described below.
  • Sebum was melted in a glass beaker in a water bath at 90°C.
  • Deoxyribonucleic acid (DNA) sodium salt from salmon testes (Sigma Aldrich) was dissolved in MilliQ water in a glass beaker to prepare a 1% (1 g/100 ml) DNA solution by stirring on a magnet stirrer for 1 hour at 500 rpm, 70°C. While stirring the DNA solution, 0.25 g lutein was added to 5 g of melted sebum, which was put in a water bath on a heat stirrer with stirring speed at 500 rpm. The sebum and lutein mix was stirred until the lutein dissolved, then added to fully dissolved DNA solution (100 ml) .
  • the sebum, lutein and DNA solution were mixed by vigorous pipetting to form an emulsion, and 1% (v/v) 80 (Sigma Aldrich) was added to the emulsion to stabilize it.
  • the mixed solution was stirred at 500 rpm in the glass beaker in a water bath at 70°C for 1 h 20 minutes. The solution had an orange color.
  • a commercial EU liquid detergent was used: Domol Colorwaschsch Violet Dream (Nopa Nordic A/S, Denmark) .
  • the stains were analyzed by SPME-GC-MS as described in Example 1 to evaluate the malodor removal benefit. Afterwards, the stains were left on a filter paper in a fume hood overnight at room temperature to evaporate malodors, and subsequently analyzed by Datacolor as described in Example 3 to evaluate the yellow color removal benefit.
  • the malodor benefit using malodor compounds 5-13 listed in Table 1, was evaluated by headspace SPME-GC-MS analysis as described in Example 1.
  • the yellow stain with DNA of this example was able to show a good yellow color removal benefit of the DNase in the liquid EU detergent, with a remission value difference of 14 units.
  • the stain was also able to show an overall malodor reduction of 68%by the DNase.

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Abstract

Provided is a technical stain test material, e.g. in the form of a swatch, comprising a substrate comprising an odor stain and/or colorant stain comprising DNA, wherein the stain comprises DNA and two or more odor compounds and/or at least one yellow colorant. The stain test material can be used to demonstrate the malodor and/or yellowing removal effect of a detergent containing a nuclease enzyme such as a DNase.

Description

TECHNICAL STAINS COMPRISING DNA FIELD OF THE INVENTION
The present invention relates to a technical stain test material, e.g. in the form of a swatch, comprising a substrate comprising an odor stain and/or a colorant stain comprising DNA.
BACKGROUND OF THE INVENTION
The use of nucleases, e.g. deoxyribonucleases, in cleaning compositions for removing biofilm and associated malodor from laundry and other surfaces has been disclosed; see e.g. WO 2014/087011, WO 2015/155350, WO 2015/155351 and WO 2015/181286, which e.g. discuss the role of DNA in microbial biofilm in textiles. When developing detergent enzymes such as nucleases aimed at removing malodor from e.g. textiles, it is necessary to have access to odor stains on which such enzymes may be tested. This is challenging however, since uniform odor stains that are able to provide a realistic assessment of the malodor removal effect of an enzyme are not readily available. Instead, it is to a large extent necessary to rely on real clothing items collected from volunteers to test the malodor removal effect of enzymes and detergents. However, real items are expensive, they have a high degree of variation, and they can be difficult to obtain. This can be exacerbated by hygiene concerns, e.g. the risk of Covid-19 infection.
A similar situation applies to discolored textiles, in particular to textiles that have lost whiteness due to greying or have developed yellowish stains in e.g. underarm areas or collars that can be difficult to remove using standard laundering processes. The presence of sweat and sebum can lead to the formation of microbial biofilms and to the development of yellowish stains on clothing. It has been found that this yellow discoloration is related to the presence of microbial biofilm, and especially to bacteria of the genus Micrococcus (Tsuchiya et al., Colloids and Surfaces B: Biointerfaces 64 (2008) 216–222) . In the case of such yellowish stains as well, it would be useful to have access to relevant technical stains, rather than having to rely on real clothing items, for the purpose of developing detergent enzymes that can remove this type of stain.
The present invention provides technical stains suitable for testing the malodor and/or yellowing removing effect of enzymes and cleaning compositions, as well as methods for making the stains and use thereof. The stains contain a mixture of compounds that simulate body grime such as sebum as well as a mixture of laundry-relevant malodor compounds and/or a yellow colorant. These technical stains can be used to demonstrate the malodor and/or yellowing removal effect of a detergent containing an enzyme, for example a nuclease enzyme such as a DNase, where malodor can e.g. be analyzed by gas chromatography-mass  spectrometry (GC-MS) and/or using human sensory evaluation, while yellow discoloration can e.g. be analyzed by determining remission values using a spectrophotometer.
SUMMARY OF THE INVENTION
The present invention relates to stain test materials, e.g. in the form of a swatch, comprising a substrate comprising an odor stain and/or a yellow stain, wherein the odor stain comprises DNA and two or more odor compounds, and optionally synthetic sebum, and wherein the yellow stain comprises DNA, synthetic sebum and at least colorant.
The invention further relates to a method for preparing the stain test materials, use of the stain test materials for testing the malodor and/or yellowing removal effect of a cleaning composition comprising an enzyme such as a nuclease, and methods for testing the malodor and/or yellowing removal effect of a cleaning composition comprising an enzyme such as a nuclease.
Definitions
For the purposes of this application, the following definitions apply.
As used herein, DNA refers to deoxyribonucleic acid from any suitable source, including but not limited to microbial DNA, e.g. bacterial or fungal DNA, or animal DNA, e.g. from a mammal or other vertebrate, for example salmon testes DNA, salmon sperm DNA, herring sperm DNA, calf thymus DNA, DNA from pork or BALB/c mouse DNA. In the case of microbial DNA, this may be in the form of extracellular DNA (eDNA) e.g. as described in Example 2 herein, or isolated bacterial or fungal DNA, for example DNA from Micrococcus luteus or from Escherichia coli.
A ” substrate” as used herein refers to a textile material which has been or may be treated with an odor stain to result in an odor stain test material. The substrate may be of any suitable textile material such as those listed further below. The substrate is typically a fabric, e.g. comprising one or more materials selected from cotton, polyester, polyacrylic, silk, wool and/or nylon, and/or other materials commonly used for fabrics. In some preferred aspects, the substrate is a cotton, polyester or a cotton/polyester blend fabric. The substrate for use in the present invention will typically be provided in the form of swatches, and the terms “substrate” and “swatch” may be used interchangeably herein.
The term “odor stain test material” , which may also be referred to as an “odor stain swatch” , refers to a test material comprising a substrate and an odor stain, where the odor stain comprises DNA and two or more odor compounds as described herein.
The term “odor compound” refers to a malodor compound with relevance for common malodors detected in laundry after wear and which may also be detected after wash. The odor compounds should have an odor (malodor) that people would generally consider to be unpleasant, they should be safe to smell so that they can be used for human sensory testing,  and they should not be easily washed off by commonly used, commercially available laundry detergents.
The terms “malodor removal” and “malodor removal effect” refer to the ability of an enzyme such as a nuclease, in particular in the form of a detergent composition comprising a nuclease, to remove malodor from a material or reduce the amount of malodor in the material. The word “removal” in the context of the term “malodor removal” includes “reduction” and should thus be understood to include any measurable reduction of malodor.
Similarly, the terms “yellowing removal” and “yellowing removal effect” refer to the ability of an enzyme such as a nuclease, in particular in the form of a detergent composition comprising a nuclease, to remove yellowing from a material or reduce the amount of yellowing in the material. The word “removal” in the context of the term “yellowing removal” includes “reduction” and should thus be understood to include any measurable reduction of yellowing.
The term “nuclease” refers to any nuclease enzyme, including enzymes with deoxyribonuclease activity (DNases) or ribonuclease activity (RNases) as well as enzymes that have both DNase and RNase activity. The odor stain test materials of the invention may be used to test the malodor removing effect of any desired nuclease.
The term “DNase” refers to a polypeptide with DNase activity that catalyzes the hydrolytic cleavage of phosphodiester linkages in the DNA backbone, thus degrading DNA.
The term “synthetic sebum” refers to a standardized synthetic sebum product that is designed to emulate natural human sebum. A number of different synthetic sebum products are commercially available, for example from Center for Testmaterials (CFT) (the Netherlands) and from wfk-Testgewebe GmbH (Germany) , as well as from other suppliers, and any such synthetic sebum product may be used in the context of the present invention. Synthetic sebum products typically comprise a mixture of components such as free fatty acids, fatty acid triglycerides, cholesterol, vegetable oil such as olive oil, tallow such as beef tallow, and hydrocarbons.
As used herein the term “fabric” (or “textile” ) refers to textile materials including non-woven materials, natural materials, synthetic materials, and any other textile material, fabrics made of these materials and products made from fabrics (e.g., garments and other articles) . The textile or fabric may be in the form of knits, wovens, denims, non-wovens, felts, yarns or toweling. The textile may be cellulose-based such as natural cellulosics, including cotton, flax/linen, jute, ramie, sisal or coir, or manmade cellulosics (e.g. originating from wood pulp) including viscose/rayon, cellulose acetate fibers (tricell) , lyocell or blends thereof. The textile or fabric may also be non-cellulose based such as natural polyamides including wool, camel, cashmere, mohair, rabbit or silk or synthetic polymers such as nylon, aramid, polyester, acrylic, polypropylene or spandex/elastane, or blends thereof as well as blends of cellulose based and non-cellulose based fibers. Examples of blends are blends of cotton and/or rayon/viscose with one or more companion material such as wool, synthetic fiber (e.g. polyamide fiber, acrylic fiber,  polyester fiber, polyvinyl chloride fiber, polyurethane fiber, polyurea fiber, aramid fiber) , such as polyester/cotton, and/or cellulose-containing fiber (e.g. rayon/viscose, ramie, flax/linen, jute, cellulose acetate fiber, lyocell) . In the context of the present invention, the terms “textile” and “fabric” may be used interchangeably.
The term “detergent composition” or “cleaning composition” refers to a composition that finds use in the removal of undesired compounds from items to be cleaned, for example textiles. Detergent compositions may be used to clean e.g. textiles for both household cleaning and industrial cleaning. The term encompasses any materials/compounds selected for the particular type of cleaning composition desired and the form of the product (e.g., liquid, gel, powder, granulate, paste, or spray compositions) and includes e.g., liquid and/or solid laundry detergents and fine fabric detergents; fabric fresheners; fabric softeners; and textile and laundry pre-spotters/pretreatment. In addition to containing a nuclease enzyme, detergent compositions may contain one or more additional enzymes (such as proteases, amylases, lipases, cutinases, cellulases, endoglucanases, xyloglucanases, pectinases, pectin lyases, xanthanases, peroxidases, haloperoxygenases, catalases and mannanases, or any mixture thereof) , and will typically contain one or more detergent adjunct ingredients such as surfactants, builders, chelators or chelating agents, bleach system or bleach components, polymers, fabric conditioners, foam boosters, suds suppressors, dyes, perfume, tannish inhibitors, optical brighteners, bactericides, fungicides, soil suspending agents, anti-corrosion agents, enzyme inhibitors or stabilizers, enzyme activators, oxido-reductases, bluing agents and fluorescent dyes, antioxidants and/or solubilizers.
The term “laundering” includes both household laundering and industrial laundering and means the process of treating textiles with a solution containing a cleaning or detergent composition. The laundering process can for example be carried out using e.g. a household or an industrial washing machine or can be carried out by hand.
The term “yellowing” (or e.g. “yellow discoloration” ) refers to discoloration of textiles, in particular clothing items that are in contact with the body, and especially areas such as collars, underarms, cuffs etc. that are subject to sebum and sweat, and where microbial biofilm that can contribute to yellow discoloration tends to form.
The term “yellow stain test material” refers to a test material comprising a substrate and a yellow stain, where the yellow stain comprises DNA, synthetic sebum and at least one yellow colorant as described herein.
The term “yellow colorant” refers to any suitable colorant with a yellowish or orange hue. The colorant will typically be a microbial, e.g. bacterial, colorant, typically a carotenoid such as lutein. Other suitable colorants include flexirubin, ankaflavin, monaphilone A, zeaxanthin, astaxanthin, β-carotene, riboflavin and azaphilones (see e.g. Aruldass et al., Journal of Cleaner Production 180 (2018) 168e182) .
The singular forms "a" , "an" and "the" include plural references unless the context clearly dictates otherwise.
Reference to “about” a value or parameter herein includes aspects that are directed to that value or parameter per se. For example, a reference to “about X” includes the aspect “X” .
The term "comprising" includes the meanings "consisting of" and "consisting essentially of” .
Unless defined otherwise or clearly indicated by context, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.
DETAILED DESCRIPTION OF THE INVENTION
The invention provides technical stains in the form of a test material, wherein the test material comprises a substrate comprising a) DNA, b) two or more odor compounds and/or at least one yellow colorant, and optionally c) synthetic sebum. As will be explained in detail in the description below, the stains may be an odor stain, a yellow stain, or a combined odor and yellow stain.
In one aspect, the present invention relates to an odor stain test material, e.g. in the form of a swatch, comprising a substrate comprising an odor stain, wherein the odor stain comprises DNA, optionally synthetic sebum, and two or more odor compounds.
In another aspect, the invention relates to a yellow stain test material, e.g. in the form of a swatch, comprising a substrate comprising a yellow stain, wherein the yellow stain comprises DNA, synthetic sebum and at least one yellow colorant.
In a further aspect the invention relates to a stain test material, e.g. in the form of a swatch, comprising a substrate comprising a combined odor stain and yellow stain, wherein the substrate comprises DNA, synthetic sebum, two or more odor compounds, and a least one yellow colorant.
The substrate of the stain test material of the invention is typically a fabric, for example comprising cotton, polyester, polyacrylic, silk, wool and/or nylon. In some embodiments, the substrate comprises cotton, polyester or a cotton/polyester blend fabric, since these are commonly used materials in clothing, towels, bedding, etc. In other embodiments, the substrate may comprise, consist essentially of or consist of synthetic polymers such as nylon, aramid, polyester, acrylic, polypropylene and spandex/elastane, or blends thereof as well as blends of one or more of these materials with cellulose based and/or non-cellulose based fibers. Synthetic materials are in some cases of particular interest for testing the malodor removing effect of a nuclease, since malodor compounds often tend to be more difficult to remove from hydrophobic synthetic materials such as polyester than from materials such as cotton, e.g. in the case of odors that are difficult to remove from athletic clothing made of synthetic materials.
Preferred substrate materials include cotton, polyester and cotton/polyester blends. In the case of a cotton/polyester blend, the fabric may e.g. contain the two materials in a ratio of from 95: 5 to 5: 95, preferably from 80: 20 to 20: 80, such as from 65: 35 to 35: 65, e.g. about 50: 50.
In one embodiment, the substrate or swatch is aged. Aging of a test swatch is used to mimic the situation where fabrics are worn or used rather than being new. Aging of a swatch can e.g. be done with the Warscator machine (Electrolux Professional FOM71CLS) by using Program 152 with tap water at 40℃, where the aging time can range from 1 hr to 24 hrs, preferably from 5 hrs to 20 hrs, more preferably from 8 hrs to 14 hrs.
As indicated above, the DNA may be from any suitable source, e.g. microbial DNA or animal DNA. In the case of animal DNA, it is apparent that this will be in the form of isolated DNA that is applied to the substrate. In contrast, in the case of microbial DNA, this may be either in the form of extracellular DNA (eDNA) obtained e.g. as described in Example 2 herein, where a swatch (i.e. the substrate) is incubated in the presence of a microorganism that produces eDNA, or it may be applied to the substrate in the form of isolated bacterial or fungal DNA.
For either isolated DNA or microbial eDNA, the amount of DNA for an individual swatch will depend on factors such as the size of the swatch and the type of DNA, and will be able to be determined by persons skilled in the art. It will be apparent that when isolated DNA is applied to a swatch, the amount of DNA will vary depending e.g. on the size of the swatch. As an example, for a 5 cm x 5 cm textile swatch with isolated DNA such as salmon testes DNA applied, a suitable amount of DNA may e.g. be in the range of about 100-1000 μl of 1%DNA, for example about 200-600 μl of 1%DNA, such as about 300-500 μl of 1%DNA. Similarly, as an example using microbial eDNA and round textile swatches with a diameter of 2 cm, a suitable amount of microbial eDNA may e.g. be provided using 1-2 ml of a starting culture of the microorganism of interest, allowing the swatches to grow in the culture under suitable conditions of temperature etc. for a suitable period of time, for example about 24 hours; see Example 2 herein. It should be emphasized that these are non-limiting examples only, as it will be apparent that the DNA, whether isolated DNA or microbial eDNA, may be provided by various means and in various amounts.
In one aspect of the invention, the odor stain comprises synthetic sebum. In a preferred embodiment of this aspect, the synthetic sebum is used in combination with isolated DNA which is applied to the substrate as a mixture containing the sebum and the DNA, and preferably an emulsifier, for example as described in Example 1. Malodor compounds bound to a mixture of sebum and DNA can be particularly difficult to remove from fabrics, thus the use of a combination of synthetic sebum and DNA allows identification of enzymes, for example nucleases such as DNases, that may be expected to be effective at removing malodor compounds in challenging situations. As explained above, synthetic sebum products are  commercially available, and the amount of sebum to be applied can therefore readily be determined by persons skilled in the art.
In another aspect of the invention, the DNA is microbial eDNA, in which case the odor stain test material may comprise synthetic sebum or it may be without synthetic sebum. Microbial eDNA contains dead bacterial cells as well as proteins and other cellular debris which contribute to malodor binding even in the absence of sebum. A substrate containing microbial eDNA can therefore perform similarly to a substrate containing isolated DNA and sebum even without the addition of synthetic sebum.
A variety of different volatile odor compounds (also referred to herein as “malodor compounds” ) may be used in the context of the present invention, in particular laundry-relevant volatile odor compounds, as long as they have an odor that most people would consider to be unpleasant, are safe to smell, and are not too easily removed from the relevant substrate material by common laundry detergents.
Volatile odor compounds are compounds that are sufficiently volatile to be transmitted via the air to the olfactory system and bind to receptors in the nose, thus being perceived as a smell or odor. While compounds such as perfume compounds are perceived as having a pleasant smell, malodor compounds are perceived as having an unpleasant smell. Such volatile compounds generally have a molecular weight of less than about 300 g/mol, typically less than about 200 g/mol.
Suitable odor compounds may include compounds from one or more categories selected from fatty acids, steroid compounds, sulfur compounds, ketones, esters, aldehydes and alcohols. In one embodiment, the odor compounds include at least one aldehyde, preferably two or more aldehydes.
Some non-limiting examples of suitable odor compounds include those from the groups listed below:
Fatty acids: for example acetic acid, propanoic acid, 2-methylpropanoic acid (isobutyric acid) , butanoic acid, 2-methylbutanoic acid, 3-methylbutanoic acid (isovaleric acid) , 3-methyl-2-hexenoic acid, 4-methyl-3-hexenoic acid (4M3H) , 5-methyl-4-hexenoic acid, 3-methyl-3-hydroxy-hexanoic acid, 6-heptenoic acid, 4-methyloctanoic acid, 4-ethyloctanoic acid, (2E) -3-methylhex-2-enoic acid.
Steroid compounds: for example 5-α-androstenol, 5-α-andro-stenone, 5-α-androst-2-en-17-one, 5-α-androst-16-ene-3-one.
Sulfur compounds: for example 3-methyl-3-sulfanyl-hexan-1-ol (3M3SH) , dimethyl disulphides, dimethyl trisulphides, benzyl mercaptan.
Ketones: for example 1-hexen-3-one, 2-heptanone, 2-octanone, 1-octen-3-one, 2-nonanone, (E) -3-octen-2-one, other medium chain ketones.
Esters: for example ethyl-2-methylpropanoate, ethyl butanoate, methyl-3-methyl hexanoate, methyl laurate, methyl myristate, 2-aminoacetophenone, diethyl phthalate.
Aldehydes: for example methional, pentanal, heptanal, hexanal, nonanal, (Z) -4-heptnal, octanal, (E) -2-octenal, cis/trans-2-nonenal, (E, Z) -2, 4-nonadienal, (E, Z) -2, 6-nonadienal, decanal, (E) -2-decenal, (E, E) -2, 4-decadienal, (E) -4, 5-epoxy-E-2-decenal, 4-methoxybenzaldehyde, 4-isopropylbenzaldehyde, cuminaldehyde, other medium-chain aldehydes.
Alcohols: for example oct-1-en-3-ol, 2-nonanol, 1-decanol, 1-dodecanol, 2-phenylethanol, 2-methoxyphenol (guaiacol) .
Examples of some preferred odor compounds for use in the present invention include the following:
● pentanal
● hexanal
● heptanal
● (Z) -4-heptenal
● octanal
● 1-octen-3-one
● nonanal
● (E) -3-octen-2-one
● trans-2-nonenal ( (E) -2-nonenal)
● (E) -2-decenal
● (E, E) -2, 4-decadienal
● decanal
● 4-isopropylbenzaldehyde
● isovaleric acid
● (2E) -3-methylhex-2-enoic acid
● 4-methyloctanoic acid
● cuminaldehyde.
For application to the substrate comprising DNA, the odor compounds may be diluted in any appropriate solvent, for example dipropylene glycol as described in the examples below.
The amount of odor compounds to be used may be readily determined by persons skilled in the art based e.g. on the nature of the substrate, the size of the swatch, and the type and amount of DNA, to result in a swatch that allows suitable differentiation of residual odor compounds after the test materials are washed using the chosen nuclease, detergent and wash conditions. As an example, for a 5 cm x 5 cm textile swatch containing DNA + synthetic sebum, a suitable amount of malodor compounds may e.g. be approximately as described in the examples, for example about 80 μl of a malodor mix such as that described in Example 1. Similarly, for a textile swatch 2 cm in diameter containing microbial eDNA, an example of suitable amount of malodor compounds may e.g. be about 10 μl of a malodor mix such as that described in Example 2. It should be emphasized that these are non-limiting examples only, as  it will be apparent that the malodor compounds may be applied in different amounts and concentrations depending on factors such as the nature of the substrate, the size of the swatch, the amount and type of DNA and optionally sebum, and the number of different malodor compounds used.
The odor stain test material of the invention comprises at least two odor compounds and will typically comprise more than two such compounds, e.g. three, four, five, six, seven, eight, nine, ten or more odor compounds, for example selected from those groups of compounds and individual compounds listed above.
In one embodiment, the odor stain comprises two or more odor compounds selected from the group consisting of pentanal, hexanal, heptanal, (Z) -4-heptenal, octanal, 1-octen-3-one, nonanal, (E) -3-octen-2-one, trans-2-nonenal, (E) -2-decenal, (E, E) -2, 4-decadienal, decanal, 4-isopropylbenzaldehyde, isovaleric acid, (2E) -3-methylhex-2-enoic acid, 4-methyloctanoic acid and cuminaldehyde. In some embodiments, the odor stain may comprise three or more such compounds, e.g. four, five, six, seven, eight or more odor compounds selected from the group consisting of pentanal, hexanal, heptanal, (Z) -4-heptenal, octanal, 1-octen-3-one, nonanal, (E) -3-octen-2-one, trans-2-nonenal, (E) -2-decenal, (E, E) -2, 4-decadienal, decanal, 4-isopropyl-benzaldehyde, isovaleric acid, (2E) -3-methylhex-2-enoic acid, 4-methyloctanoic acid and cuminaldehyde.
In some embodiments, the odor stain test material may further comprise an emulsifier, in particular where the odor stain includes synthetic sebum. Suitable emulsifiers include those that can help to form oil-in-water (O/W) emulsions, preferably emulsifiers with a Hydrophilic-Lipophilic Balance (HLB) value in the range of between about 8 and 16. The emulsifier may, e.g., be a polysorbate emulsifier having a suitable HLB value, for example polysorbate 80 (polyoxyethylene sorbitan monooleate, for example 
Figure PCTCN2022123105-appb-000001
80) .
In a further aspect, the invention relates to a method for preparing an odor stain test material, the method comprising applying DNA, two or more odor compounds and optionally an emulsifier to a substrate. In this aspect, the substrate and the odor compounds may comprise any of those described above. Preferably, the method involves applying a mixture of DNA, synthetic sebum and optional emulsifier to the substrate prior to application of the odor compounds.
In one embodiment, the stain comprises both DNA and artificial sebum as explained above. In this case, when the DNA is isolated DNA, the DNA and sebum may be applied to the substrate separately, but they are preferably applied as a mixture of DNA and synthetic sebum, typically in the form of an emulsion prepared using any suitable emulsifier as mentioned above. The DNA, or DNA and artificial sebum, is typically applied to the substrate and then dried, for example drying at room temperature e.g. overnight, before the odor compounds are applied to the substrate. An example of preparation of odor stain swatches comprising isolated DNA and artificial sebum is provided in Example 1 herein.
The DNA can be supplied in the form of powders or liquid solutions and can be formulated as needed. A DNA solution can be prepared by dissolving the DNA in water or another suitable solvent or buffer. For preparing the odor stain swatches, a DNA solution may, for example, be provided in the concentration of from 0.1 mg/mL to 20 mg/mL, for example from 5 mg/mL to 15 mg/mL, e.g. from 8 mg/mL to 12 mg/mL.
In another embodiment, the DNA is eDNA obtained by incubating the substrate (e.g. a fabric swatch) in the presence of a microorganism that produces eDNA under conditions that allow for growth of the microorganism and deposit of eDNA on the substrate. The substrate is then typically rinsed with water and then dried, e.g. at room temperature, before application of odor compounds to the dry substrate. Artificial sebum may optionally be added to the substrate, e.g. before or after rinsing the substrate following microorganism growth, or prior to microorganism growth. As mentioned above, however, a substrate containing microbial eDNA can be without sebum.
In further aspects, the invention relates to 1) use of an odor stain test material as described herein for testing the malodor removal effect of a cleaning composition comprising an enzyme such as a nuclease; and 2) a method for testing the malodor removal effect of a cleaning composition comprising an enzyme such as a nuclease, the method comprising providing an odor stain test material as described herein, subjecting the odor stain test material to washing with a cleaning composition comprising a nuclease or other enzyme, and evaluating residual malodor on the odor stain after wash.
In these aspects, an odor stain test material, which has been prepared as described herein using any of the methods and materials disclosed above or e.g. in the examples below, is washed with a cleaning composition comprising a nuclease, after which the malodor removal effect of the cleaning composition comprising a nuclease is determined by measuring the residual malodor after wash.
A yellow stain test material may be prepared substantially as described above for the odor stain test material in relation to the DNA component and the synthetic sebum component. However, instead of or in addition to the two or more odor compounds, the yellow stain test material will comprise at least one yellow colorant.
As indicated above, the yellow colorant may be any suitable colorant with a yellowish or orange hue, and will typically be a microbial, e.g. bacterial, colorant, typically a carotenoid, such as lutein. Other suitable colorants include flexirubin, ankaflavin, monaphilone A, zeaxanthin, astaxanthin, β-carotene, riboflavin and azaphilones. Preferably, the yellow colorant is a carotenoid, with a preferred carotenoid being lutein.
Carotenoids, for example lutein, zeaxanthin and β-carotene, are hydrophobic molecules. The yellow stain test materials of the invention therefore preferably contain an emulsifier. This allows the hydrophilic DNA to be more readily mixed with the hydrophobic sebum and colorant  and applied to a textile. The emulsifier may be as described above in the context of the odor stain test material, for example a polysorbate emulsifier such as polysorbate 80.
In further aspects, the invention relates to 1) use of a yellow stain test material as described herein for testing the yellowing removal effect of a cleaning composition comprising an enzyme such as a nuclease; and 2) a method for testing the yellowing removal effect of a cleaning composition comprising an enzyme such as a nuclease, the method comprising providing a yellow stain test material as described herein, subjecting the yellow stain test material to washing with a cleaning composition comprising a nuclease or other enzyme, and evaluating the whiteness of the yellow stain after wash.
In these aspects, a yellow stain test material, which has been prepared as described herein using any of the methods and materials disclosed above or e.g. in the examples below, is washed with a cleaning composition comprising a nuclease, after which the yellowing removal or reduction effect of the cleaning composition comprising a nuclease is determined by measuring the whiteness of the yellow stain after wash.
In a preferred embodiment, the stain test material of the invention comprises both an odor stain and a yellow stain in the form of a combined odor and yellow stain. In this case, the substrate comprises a combined odor and yellow stain, wherein said stain comprises DNA, two or more odor compounds, at least one yellow colorant, synthetic sebum, and preferably an emulsifier. An example of such a stain test material comprising a combined odor and yellow stain and use thereof is provided in Example 4.
In a further aspect, the invention relates to a method for preparing a yellow stain test material, the method comprising applying DNA, synthetic sebum, at least one yellow colorant and preferably an emulsifier to a substrate. In this aspect, the substrate and yellow colorant may comprise any of those described above. The method may e.g. be performed by first forming a mixture of DNA, synthetic sebum, yellow colorant and emulsifier, whereafter this mixture is applied to the substrate. In the case of a combined odor and yellow stain, the odor compounds may be suitably applied to the substrate after application of the mixture of DNA, synthetic sebum, yellow colorant and emulsifier.
In an embodiment, divalent ions such as magnesium or calcium ions may be included in the yellow stain, odor stain or combined yellow and odor stain. This serves to reduce the stain’s sensitivity to DNase, which has been found to better mimic the effect of DNase on real laundry items, thus allowing for a more realistic assessment of the ability of different DNases to remove yellow and/or odor stains and a better dose response to addition of DNase. Preferred divalent ions include magnesium ions, e.g. in the form of magnesium chloride (MgCl 2) . Calcium ions, e.g. in the form of calcium chloride (CaCl 2) , may also be used. For a yellow stain, the divalent ions may be added to the mixture of DNA, synthetic sebum, yellow colorant and preferably an emulsifier. Similarly, for an odor stain, the divalent ions may e.g. be added a mixture of DNA, odor compounds, optional synthetic sebum and optional emulsifier. For magnesium ions, these  may be added to result in a concentration of magnesium chloride in the range of from about 1 mM to about 40 mM, for example from about 2 mM to about 20 mM, such as from about 5 mM to about 15 mM, such as about 10 mM. For calcium ions, these may be added to result in a concentration of calcium chloride in the range of from about 1 mM to about 500 mM, such as from about 5 to about 50 mM.
Testing the malodor or yellowing removal effect of a cleaning composition comprising a nuclease using an odor stain test material of the invention is not limited to any particular nuclease or cleaning composition. Rather, any nuclease in any desired cleaning/detergent composition may be tested in order to determine the malodor removal effect of a chosen nuclease in a chosen composition.
The nucleases that may be tested using the stain test materials and methods of the invention may include DNases, RNases, and enzymes with both DNase and RNase activity. In one embodiment, the nuclease is a DNase, including DNase polypeptides derived from bacteria and fungi, as well as genetically engineered variants thereof. Non-limiting examples of such DNases include DNases derived from Aspergillus, e.g. Aspergillus oryzae, such as those disclosed in WO 2015/155350, DNases derived from Trichoderma, e.g. Trichoderma harzianum, such as those disclosed in WO 2015/155351, DNases derived from genera formerly classified as Bacillus, e.g. Metabacillus indicus (formerly known as Bacillus cibi) , such as those disclosed in WO 2017/060475, DNases derived from Vibrissea, e.g. Vibrissea flavovirens, such as those disclosed in WO 2017/060493, DNases derived from Morchella, e.g. Morchella costata, such as those disclosed in WO 2018/177203, and DNases derived from Rhizoctonia, e.g. Rhizoctonia solani, such as those disclosed in WO 2018/177938. Genetically engineered variants of any of these DNases are also included, for example variants of a DNase from Aspergillus oryzae such as those disclosed in WO 2017/064269, and variants of a DNase from Metabacillus indicus (formerly known as Bacillus cibi) such as those disclosed in WO 2018/011277, WO 2019/081724 and WO 2019/081721.
A preferred method for analysis of residual malodor of an odor stain swatch of the invention is by chromatography-mass spectrometry (GC-MS) . GC-MS combines gas chromatography to separate the compounds in a chemical mixture and mass spectrometry to identify the compounds at the molecular level. GC-MS is particularly applicable to analysis of volatile compounds, and is therefore well-suited to analyzing volatile odor compounds that are the cause of malodor on e.g. textiles. GC-MS analysis is a technique which is well-known to persons skilled in the art, and instruments for GC-MS analysis are commercially available.
Additionally or alternatively, residual malodor may be analyzed by human sensory evaluation, e.g. using persons specially trained to detect odors and/or a panel of consumer test persons.
Determination of the yellowing removal or reduction effect may suitably be performed by measuring the remission values using a spectrophotometer, e.g. as described in the examples  below. The remission values are correlated with the “whiteness” of the fabric and thus with the degree to which the yellow stain has been removed.
The invention is further described in the following non-limiting examples.
EXAMPLES
Example 1: Stain containing sebum, DNA and malodor to show malodor removal effect in detergents with a DNase
Fabric
Knitted cotton fabric (CN-42, Center For Testmaterials BV, the Netherlands) was prewashed as described below and cut into pieces of 5 cm × 5 cm.
Prewash
Prewash of textiles was done primarily to remove starch, carboxymethyl cellulose (CMC) and other additives from the textiles. Three kg of textile fabrics were washed three times in 78.6 g of W-ECE-2 detergent (wfk Testgewebe GmbH, Germany) using water with 15°dH water hardness [2.139 mM CaCl 2, 0.5355 mM MgCl 2, 4.0125 mM NaHCO 3 dissolved in deionized water] and containing the following enzymes (available from Novozymes A/S, Denmark) for each of the three prewash steps :
Figure PCTCN2022123105-appb-000002
Prewashing was carried out in a Miele Softtronic W1935 WTL machine at 40℃ using a standard washing program with 13-15 liters of water for 3 kg of textile. After the third prewash step, a rinse was carried out in deionized water and textile fabrics were then line dried.
Malodor
All malodor compounds (Table 1) were purchased from Sigma Aldrich. They were diluted in dipropylene glycol (CAS Number 25265-71-8, Sigma Aldrich) . For the experiments with liquid detergents (Tables 2 and 3) , compounds 1-11 were used, and they were mixed to reach a final concentration of 200 mM for each compound. For the experiments with powder  detergents (Tables 4 and 5) , compounds 5-13 were used, and they were mixed to reach a final concentration of 5mM for compounds 5-12 and 80 mM for compound 13.
Table 1. Malodor compounds used to prepare odor stain
Figure PCTCN2022123105-appb-000003
* Examples of suitable acid malodor compounds, not used in the present examples
Preparation of odor swatches
Preparing DNA and sebum stain
Deoxyribonucleic acid (DNA) sodium salt from salmon testes (Sigma Aldrich) was dissolved in MilliQ water in a 50 ml falcon tube to prepare a 1%DNA solution by rotating the tubes at 20 rpm for 1 h. While rotating the DNA solution, artificial sebum (BEY sebum, wfk-Testgewebe GmbH, Germany) was melted in a glass beaker in a water bath at 90℃. After DNA was fully dissolved and the sebum was melted, 1.6 ml sebum was transferred into 30 ml 1%DNA in a glass beaker with magnetic stirring. Sebum and DNA solution were mixed by vigorous pipetting to form an emulsion, and 0.02% (v/v) 
Figure PCTCN2022123105-appb-000004
80 (Sigma Aldrich) was added to the emulsion to stabilize it. The glass beaker was put in a water bath on a heat stirrer with a stirring speed of 400 rpm. To avoid water evaporation during stirring, the glass beaker was covered with tin foil.
400 μl of the DNA and sebum emulsion was then applied to the center of the cotton swatches to make the DNA and sebum stain, which was subsequently dried over night at room temperature without direct sunlight.
Adding malodor compounds to the DNA and sebum stain
After overnight drying, the DNA and sebum stains were placed in a fume hood. 80 μl of the malodor mix was added to the center of each DNA and sebum stain to make the odor stain, which was left in the fume hood for 1 h before washing.
Detergents
Two commercial liquid detergents and two commercial powder detergents from different EU areas were tested: Ropa Blanca Y Color (Inquiba, Spain; liquid) , Gut &Günstig Aktiv Vollwaschmittel (McBride, Germany; liquid) , Persil Color 360° (Henkel, Germany; powder) , and Persil Universal Pulver Tiefenrein (Henkel, Germany; powder) .
Wash assay
1. Terg-O-tometer (TOM) wash
The Tergotometer consists of sixteen 2 L metal beakers, each fitted with an agitator which rotates in a back-and-forth manner at a controlled speed (120 rpm) to simulate the agitation mode occurring in commercial top-loader washing machines. The beakers were partly submerged in thermostatic water baths where the temperature was kept at 30℃. Four stains were washed in one beaker filled with 1 L detergent solution (15 dH water with 5.1 g/L Ropa Blanca Y Color or with 5.3 g/L Gut &Günstig Aktiv Vollwaschmittel) , and 1.0 ppm DNase was added to each beaker. The DNase was a fungal DNase derived from Aspergillus oryzae (mature polypeptide of SEQ ID NO: 2 as disclosed in WO 2015/155350) . Washes without  enzyme were included as controls. Two replicates (1 replicate/beaker) were used for each condition (detergent/DNase combination) .
After a wash period of 20 minutes, the stains were promptly removed from the beakers and put back to the same beaker to be rinsed with 1 L 15°dH water for 5 minutes.
2. Drying of stains
After washing and rinsing, each odor stain was centrifuged in a falcon tube containing a filter (HOZELOCK K3 Kaldness filter, Aquacadabra, eBey) at 4000 g for 5 min to remove excess water. This plastic filter separated the excess water from the swatch after centrifuging.
3. Evaluation by headspace SPME-GC-MS analysis
To analyze the malodor left on the stain after wash, 4 centrifuged stains from the same beaker were placed in one 20 mL GC-MS vial (Mikrolab Aarhus A/S, Aarhus, Denmark) and capped with silicone screw top lids (Mikrolab Aarhus A/S, Aarhus, Denmark) . Samples were analyzed in in randomized order, with the headspace from each tube being analyzed in a GCMS Agilent 7890 GC with split/splitless injector and 5977 MS with extractor ion source coupled to a Gerstel MPS2 sampler with HS/SPME, SPME needle heater.
The method used was:
● GC oven temperature: initial 40℃; hold 2 min; rate 5℃/min until 180℃; rate 30℃/min until 240℃; hold 0 min. Front SS Inlet He: Mode Split; T a 230℃, Split Ratio 10: 1; Split Flow 15 mL/min. Column: Agilent 19091F-433: FFAP-01 HP-FFAP 30 m x 250 μm x 0.25 μm.
● Gerstel MPS SPME Incubator: Agitator. incubation temperature: 60℃; incubation time: 10.00 min; agitator speed: 250 rpm; sample parameters: extraction time: 2.00 min; inj. desorption time: 120 s.
● Fiber type: carboxen/Polydimethylsiloxane (CAR/PDMS)
● MS Information: Acquisition Mode: Scan. Solvent Delay (minutes) : 1. Scan Parameters: Start Time: 1. Low Mass: 35. High Mass: 350. Threshold: 100. A/D Samples: 4. MSZones: MS Source: 230℃. MS Quad: 150℃
Results
The data below show that Gut &Günstig Aktiv Vollwaschmittel detergent removed more malodor than Ropa Blanca Y Color detergent in the absence of the DNase, but still a large amount of malodor was left after wash. The use of DNase resulted in a substantially lower level of malodor in both liquid detergents, with an overall reduction of 77.2%and 41.3%, respectively, in Ropa Blanca Y Color and Gut &Günstig Aktiv Vollwaschmittel (Tables 2 and 3) .
The DNase also showed good malodor removal benefit in the two powder detergents, Persil Color 360° and Persil Universal Pulver Tiefenrein, with an overall reduction of 38.1%and 52.7%, respectively. It is noteworthy that Persil Universal Pulver Tiefenrein contains 15-30%oxygen-based bleach in its formulation. Thus the results show that the DNase is also effective in reducing malodor in bleach-containing detergents.
Table 2. Malodor intensity after wash with Ropa Blanca Y Color liquid detergent without and  with a DNase. The malodor mix contained compounds 1-11 listed in Table 1.
Figure PCTCN2022123105-appb-000005
Table 3. Malodor intensity after wash with Gut &Günstig Aktiv Vollwaschmittel liquid detergent  without and with a DNase. The malodor mix contained compounds 1-11 listed in Table 1.
Figure PCTCN2022123105-appb-000006
Figure PCTCN2022123105-appb-000007
Table 4. Malodor intensity after wash with Persil Color 360° powder detergent without and with  a DNase. The malodor mix contained compounds 5-13 listed in Table 1.
Figure PCTCN2022123105-appb-000008
N.D., not detected; N.A., not applicable
Table 5. Malodor intensity after wash with Persil Universal Pulver Tiefenrein powder detergent  without and with a DNase. The malodor mix contained compounds 5-13 listed in Table 1.
Figure PCTCN2022123105-appb-000009
N.D., not detected; N.A., not applicable
Example 2: Stain containing microbial extracellular DNA and malodor to show malodor removal effect in detergents with a DNase
Fabric
Knitted cotton fabric (CN-42, Center For Testmaterials BV, the Netherlands) was pre-washed and cut into round pieces with diameter of 2 cm.
Malodor
All malodor compounds (Table 6) were purchased from Sigma Aldrich. They were diluted in dipropylene glycol (CAS Number 25265-71-8, Sigma Aldrich) and mixed to reach a final concentration of 40 mM for each compound.
Table 6. Malodor compounds used to prepare odor stain
Figure PCTCN2022123105-appb-000010
Preparation of swatches of the invention
Preparation of microbial extracellular DNA (eDNA) stain
Pseudomonas fluorescens was pre-grown on Tryptone Soya Agar (TSA) (pH 7.3) (CM0131, Oxoid Lid. Basurgstukc, UK) for 2-5 days at 30℃. From a single colony, a loop-full was transferred to 10 mL of Tryptone Soya Broth (TSB) and incubated for 1 day at 30℃ with shaking (240 rpm) . After propagation, Pseudomonas fluorescens was pelleted by centrifugation (Sigma Laboritory Centrifuge 6K15) (3000 g at 21 ℃, in 7 min) and resuspended in 10 mL of TSB diluted twice with water. Optical density (OD) at 600 nm was measured using a spectrophometer (POLARstar Omega (BMG Labtech, Ortenberg, Germany) . Fresh TSB diluted twice with water was inoculated to an OD 600nm of 0.03, and 1.6 ml was added into each well of a  12-well polystyrene flat-bottom microplate (3512; Corning Incorporated, Corning, N. Y., USA) in which a round swatch (diameter 2 cm) of sterile CN-42 was placed. After incubation (24 h at 15℃, with shaking at 100 rpm) , swatches were rinsed twice with 0.9% (w/v) NaCI.
Adding malodor compounds to the microbial eDNA stain
After rinsing, the stains were left on a plastic tray in a flow cabinet to dry at room temperature for 24 h. Then the tray was moved to a fume hood and 10 μl of malodor mix (Table 6) was added to the center of each stain. The odor stain was left in the fume hood for 1 h before washing.
Detergents
Wash solutions of liquid model detergent A2 or powder model detergent T (without bleach) were prepared by weighing the detergents and dissolving them in water with 15°dH water. Dosing of model detergent A and T (without bleach) was 3.33 g/L and 5.3 g/L, respectively.
Composition of Model Detergent A2 (liquid)
Ingredients: 12%Sodium salt of linear alkylbenzene sulphonates (Na-LAS) , 12%alcohol ethoxylate (AEO) Biosoft N25-7 (NI) , 4%alkyl ethoxysulfate /sodium laureth sulfate (AEOS/SLES) , 2%MPG (monopropylene glycol) , 3.1%ethanol, 2%triethanolamine (TEA) , 3%palm kernel oil soap, 2%sodium hydroxide, 3.9%sodium citrate, 1.5%diethylenetriaminepenta (methylene phosphonic acid) (DTMPA) and 0.5%phenoxyethanol (all percentages are w/w) .
Composition of Model Detergent T (without bleach, powder)
Ingredients: 11%LAS, 2%alkyl sulfate (AS) /alkyl ethoxysulfate (AEOS) , 2%soap, 3%alcohol ethoxylate (AEO) , 15.15%sodium carbonate, 3%sodium slilcate, 18.75%zeolite, 0.15%chelant, 2%sodium citrate, 1.65%copoly (acrylic acid/maleic acid) (AA/MA) copolymer, 2.5%(carboxymethyl) cellulose (CMC) and 0.5%soil-release polymer (SRP) (all percentages are w/w) .
Wash assay
Mini Launder-O-Meter (MiniLOM) Wash
The miniLOM assay uses a small-scale version of a
Figure PCTCN2022123105-appb-000011
 (LOM) . A miniLOM basically consists of closed test tubes which are rotated in a heating cabinet for a given time and at a given temperature. Each test tube represents one small washing machine. It is mainly used in testing of detergents and enzymes at European wash conditions.
In this experiment, three odor stains were placed in a 50 ml glass tube, and 20 ml of wash solution (15 dH water with 3.15 g/L Model A2 or 5.3 g/L Model T (without bleach) ) and  1.0 ppm DNase were added to each tube. The DNase was a fungal DNase derived from Aspergillus oryzae (mature polypeptide of SEQ ID NO: 2 as disclosed in WO 2015/155350) . Washes without DNase were included as controls. Three replicates (one replicate/tube) were used for each condition (detergent/DNase combination) . To better mimic the wash performance of commercial detergents in Europe, an enzyme blend, 
Figure PCTCN2022123105-appb-000012
Brilliant (Novozymes A/S, Denmark) , was added to all glass tubes at the dosage of 2.5%of the detergent. The enzyme blend contains a variety of commonly used enzymes, including protease, amylase, lipase, mannanase, pectate lyase and two cellulases.
The glass tubes were then mounted in a mini-Launder-Ometer (aStuart Tube Rotator SB3) and washed at 30℃ for 60 minutes at 20 rpm. After wash, the rotator was placed at room temperature while swatches from one glass tube at a time were rinsed with 15°dH water and placed back into the rotator. Each glass tube was rinsed twice in 30 mL 15°dH water.
Drying of stains and evaluation by headspace SPME-GC-MS analysis
After washing and rinsing, the stains were dried and evaluated as described above in Example 1.
Results
The results below show that the use of DNase in both liquid model detergent A2 and powder model detergent T (without bleach) resulted in a substantially greater removal of malodor, with a reduction of 56.2%and 24.5%, respectively, for the model A2 and model T detergents (Tables 7 and 8) .
Table 7. Malodor intensity on odor stains washed in liquid model detergent A2 without and with  a DNase
Figure PCTCN2022123105-appb-000013
Table 8. Malodor intensity on odor stains washed in powder model detergent T without and with  a DNase
Figure PCTCN2022123105-appb-000014
Example 3: Stain containing sebum, DNA and yellow colorant to show yellow color removal effect in detergent with a DNase
Fabric
The textile fabrics used were cotton (W-80A) , polyester/cotton (polycotton) (T-7422) , polyester (T720) . All fabrics were purchased from Center For Testmaterials B.V, The Netherlands. The fabrics were prewashed as described above in Example 1 before and cut into pieces of 6 cm × 6 cm.
Preparation of swatches
In addition to DNA, sebum and
Figure PCTCN2022123105-appb-000015
80 used in the above odor stain, lutein (CAS Number 127-40-2, Shanghai Aladdin Bio-Chem Technology Co., LTD, China) was added to the mixture to provide yellow color to the stain. The specific procedure is described below.
Artificial sebum (BEY sebum, wfk-Testgewebe GmbH, Germany) was pre-aged in an oven at 180℃ for 3 days. Deoxyribonucleic acid (DNA) sodium salt from salmon testes (Sigma Aldrich) was dissolved in MilliQ water in a glass beaker to prepare a 1% (1 g/100 ml) DNA solution by stirring on a magnet stirrer for 1 hour at 500 rpm, 70℃. This experiment was run as two separate trials, one of which included magnesium chloride hexahydrate (Sigma Aldrich) added to the DNA solution to a final MgCl 2 concentration of 10 mM. While stirring the DNA solution, 0.25 g lutein was added to 5 g of pre-aged sebum, which was put in a water bath on a heat stirrer with a stirring speed at 500 rpm. The sebum and lutein mix was stirred until the lutein dissolved, efter which the sebum and lutein mix was added to the to fully dissolved DNA  solution (100 ml) . The sebum, lutein and DNA solution were mixed by vigorous pipetting to form an emulsion, and 1% (v/v) 
Figure PCTCN2022123105-appb-000016
80 (Sigma Aldrich) was added to the emulsion to stabilize it. The mixed solution was stirred at 500 rpm in the glass beaker in a water bath at 70℃ for 20 minutes. The solution had an orange color.
600 μL, 600 μL or 400 μL of the mix solution was applied to cotton, polycotton or polyester swatches, respectively. Fabrics were picked up one at a time and place on the lid of 50 mL tubes, stretching the fabric with fingers, and the mix solution was smeared evenly using a finger for thirty cycles in a clockwise direction. The swatches were subsequently dried overnight at room temperature without direct sunlight.
Detergents
A commercial EU liquid detergent was used: Domol Colorwaschmittel Violet Dream (Nopa Nordic A/S, Denmark) .
Wash assay
Washes were performed in water with 15°dH [2.139 mM CaCl 2, 0.5355 mM MgCl 2, 4.0125 mM NaHCO 3 dissolved in MilliQ water] in a Terg-O-tometer (TOM) instrument. The TOM instrument consisted of 1 L beakers with agitator spindles inserted into the beakers and rotated in a back-and forth manner at a controlled speed (120rpm) to simulate a commercial top-loader/vertical drum washing machine. The beakers were immersed in a temperature-controlled water bath at 30℃ during wash. The detergent was dissolved in 15°dH water at a concentration of 3.5 g/L.
To each beaker, two swatches from each of the above three fabric types were added together with one-third of an SBL2004 sheet (Center For Testmaterials B. V, The Netherlands) and some clean prewashed 80A textile (Center For Testmaterials B. V, The Netherlands) . The textile in each beaker weighed 30 g in total, and they were washed in 1 L detergent solution to which 0.5 ppm DNase had been added. The DNase was a stabilized variant of the fungal DNase used in Example 1. Washes without enzyme were included as controls.
After a wash period of 20 minutes, the stains were promptly removed from the beakers and rinsed thoroughly under running deionized water for 30 sec. Then they were allowed to air dry on filter paper overnight at room temperature or until dry. The stains were yellow or pale yellow after washing and drying.
Evaluation of the yellow color removal benefits of DNase
To assess the yellow color removal benefits of DNase on textiles, the dried stains were measured for whiteness on a Datacolor 800V spectrophotometer (Datacolor, Lawrenceville, NJ, USA) under the CIE Standard Illuminant D65 and the CIE 1964 10-degree Standard Observer.  The measurements were made without UV in the incident light, and remission values at 460 nm were recorded for textile traces washed with and without DNase. The yellow color removal benefits were evident when the differences in remission between stains washed with and without DNase were higher than two units. For these remission value differences, the human eye is be able to detect the yellow color removal benefits between stains.
Results
The yellow stains with DNA of this example were able to show a good yellow color removal benefit of the DNase in the liquid EU detergent for all fabric types, and both with and without magnesium ions, with remission value differences that were easily distinguishable to the human eye. The results for the different fabrics are provided below in Tables 9 and 10, where Table 10 shows the results including 10 mM Mg 2+ and Table 9 shows the results without Mg 2+.
Table 9: Remission values (460 nm) measured for yellow stains washed in TOM without and  with a DNase in liquid EU detergent (Domol Colorwaschmittel Violet Dream)
Figure PCTCN2022123105-appb-000017
Table 10:  Remission values (460 nm) measured for yellow stains (containing 10 mM Mg 2+ washed in TOM without and with a DNase in liquid EU detergent (Domol Colorwaschmittel  Violet Dream)
Figure PCTCN2022123105-appb-000018
Example 4: Stain containing sebum, DNA, yellow colorant and malodor to show yellow color and malodor removal effect in detergent with a DNase
Fabric
Knitted cotton fabric (CN-42) was pre-washed as described above and cut into pieces of 5 cm × 5 cm.
Preparation of swatches
The stain was prepared as described above in Example 3, with two small differences: 1) the sebum was not pre-aged; 2) the mix of DNA, sebum and yellow color was not applied to the  fabric by finger, but by pipetting to the center of the swatches as in Examples 1 and 2. The specific procedure is described below.
Sebum was melted in a glass beaker in a water bath at 90℃. Deoxyribonucleic acid (DNA) sodium salt from salmon testes (Sigma Aldrich) was dissolved in MilliQ water in a glass beaker to prepare a 1% (1 g/100 ml) DNA solution by stirring on a magnet stirrer for 1 hour at 500 rpm, 70℃. While stirring the DNA solution, 0.25 g lutein was added to 5 g of melted sebum, which was put in a water bath on a heat stirrer with stirring speed at 500 rpm. The sebum and lutein mix was stirred until the lutein dissolved, then added to fully dissolved DNA solution (100 ml) . The sebum, lutein and DNA solution were mixed by vigorous pipetting to form an emulsion, and 1% (v/v) 
Figure PCTCN2022123105-appb-000019
80 (Sigma Aldrich) was added to the emulsion to stabilize it. The mixed solution was stirred at 500 rpm in the glass beaker in a water bath at 70℃ for 1 h 20 minutes. The solution had an orange color.
400 μL of the mix solution was applied to the center of the cotton swatches (CN-42) , which were subsequently dried overnight at room temperature without direct sunlight.
Adding malodor compounds to the DNA, sebum and yellow color stain
After overnight drying, the DNA and sebum stains were placed in a fume hood. 80 μl of the malodor mix was added to the center of each DNA and sebum stain to make an odor stain, which was left in the fume hood for 1 h before washing.
Detergent
A commercial EU liquid detergent was used: Domol Colorwaschmittel Violet Dream (Nopa Nordic A/S, Denmark) .
Wash assay
Washes were performed in 15°dH water [2.139 mM CaCl 2, 0.5355 mM MgCl 2, 4.0125 mM NaHCO 3 dissolved in MilliQ water] in a Terg-O-tometer (TOM) instrument as described in Example 1. In this experiment, 0.5 ppm DNase was added to each beaker, and washes without enzyme were included as controls. The DNase was a stabilized variant of the DNase used in Example 3. After wash, the stained swatches were dried as described in Example 1.
Evaluation of yellow color and malodor removal benefits of DNase
After drying by centrifugation, the stains were analyzed by SPME-GC-MS as described in Example 1 to evaluate the malodor removal benefit. Afterwards, the stains were left on a filter paper in a fume hood overnight at room temperature to evaporate malodors, and subsequently analyzed by Datacolor as described in Example 3 to evaluate the yellow color removal benefit.
The malodor benefit, using malodor compounds 5-13 listed in Table 1, was evaluated by headspace SPME-GC-MS analysis as described in Example 1.
Results
The yellow stain with DNA of this example was able to show a good yellow color removal benefit of the DNase in the liquid EU detergent, with a remission value difference of 14 units. The stain was also able to show an overall malodor reduction of 68%by the DNase.
Table 11: Remission values (460 nm) measured for yellow odor stains washed in TOM without  and with a DNase in liquid EU detergent (Domol Colorwaschmittel Violet Dream)
Figure PCTCN2022123105-appb-000020
Table 12. Malodor intensity after wash in EU detergent (Domol Colorwaschmittel Violet Dream)  without and with a DNase.
Figure PCTCN2022123105-appb-000021

Claims (14)

  1. A stain test material, comprising a substrate comprising a) DNA, b) two or more odor compounds and/or at least one yellow colorant, and optionally c) synthetic sebum.
  2. The test material of claim 1, comprising a substrate comprising an odor stain, wherein the odor stain comprises DNA and two or more odor compounds.
  3. The test material of claim 2, wherein the odor stain further comprises synthetic sebum.
  4. The test material of any of the preceding claims, wherein the odor compounds comprise at least one aldehyde; for example wherein the odor compounds comprise at least two compounds selected from the group consisting of pentanal, hexanal, heptanal, (Z) -4-heptenal, octanal, 1-octen-3-one, nonanal, (E) -3-octen-2-one, trans-2-nonenal, (E) -2-decenal, (E, E) -2, 4-decadienal, decanal, 4-isopropylbenzaldehyde, isovaleric acid, (2E) -3-methylhex-2-enoic acid, 4-methyloctanoic acid and cuminaldehyde.
  5. The test material of any of the preceding claims, wherein the odor stain comprises three, four, five, six, seven, eight or more odor compounds; preferably wherein the odor stain comprises three, four, five, six, seven, eight or more of the odor compounds listed in claim 4.
  6. The test material of any of the preceding claims, comprising a substrate comprising a yellow stain, wherein the yellow stain comprises DNA, synthetic sebum and at least one yellow colorant.
  7. The test material of claim 6, wherein the yellow colorant is a carotenoid, preferably lutein, or is selected from the group consisting of flexirubin, ankaflavin, monaphilone A, zeaxanthin, astaxanthin, β-carotene, riboflavin and azaphilones.
  8. The test material of any of the preceding claims, wherein the substrate is a fabric, e.g. comprising cotton, polyester, polyacrylic, silk, wool and/or nylon; preferably wherein the substrate is a cotton, polyester or cotton/polyester fabric.
  9. The test material of any of the preceding claims, wherein the odor and/or yellow stain further comprises an emulsifier, e.g. a polysorbate such as polysorbate 80.
  10. The test material of any of the preceding claims, wherein the substrate comprises a combined odor and yellow stain, wherein said stain comprises DNA, two or more odor compounds, at least one yellow colorant, synthetic sebum, and preferably an emulsifier.
  11. The test material of any of the preceding claims, wherein the yellow stain, odor stain or combined yellow and odor stain further comprises divalent ions, preferably magnesium ions.
  12. Use of a test material as defined in any of claims 1-11 for testing the malodor and/or yellowing removal effect of a cleaning composition comprising a nuclease or other enzyme.
  13. A method for testing the malodor removal effect of a cleaning composition comprising an enzyme such as a nuclease, the method comprising providing an odor stain test material as defined in any of claims 1-5 or 8-11, subjecting the odor stain test material to washing with a cleaning composition comprising a nuclease or other enzyme, and evaluating residual malodor on the odor stain after wash.
  14. A method for testing the yellowing removal effect of a cleaning composition comprising an enzyme such as a nuclease, the method comprising providing a yellow stain test material as defined in any of claims 1 or 6-11, subjecting the yellow stain test material to washing with a cleaning composition comprising a nuclease or other enzyme, and evaluating the whiteness of the yellow stain after wash.
PCT/CN2022/123105 2021-10-08 2022-09-30 Technical stains comprising dna WO2023056892A1 (en)

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