CN113966745A - Botanical preparation for preventing and treating red spiders and application thereof - Google Patents

Botanical preparation for preventing and treating red spiders and application thereof Download PDF

Info

Publication number
CN113966745A
CN113966745A CN202111339597.9A CN202111339597A CN113966745A CN 113966745 A CN113966745 A CN 113966745A CN 202111339597 A CN202111339597 A CN 202111339597A CN 113966745 A CN113966745 A CN 113966745A
Authority
CN
China
Prior art keywords
red spiders
preparation
citrus
botanical
plant
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202111339597.9A
Other languages
Chinese (zh)
Inventor
吴王洋
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Guangxi Zhendi Biotechnology Co ltd
Original Assignee
Guangxi Zhendi Biotechnology Co ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Guangxi Zhendi Biotechnology Co ltd filed Critical Guangxi Zhendi Biotechnology Co ltd
Priority to CN202111339597.9A priority Critical patent/CN113966745A/en
Publication of CN113966745A publication Critical patent/CN113966745A/en
Pending legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G13/00—Protecting plants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00—Botany in general
    • A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
    • A01N31/02—Acyclic compounds
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/18—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group —CO—N<, e.g. carboxylic acid amides or imides; Thio analogues thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
    • A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N45/00—Biocides, pest repellants or attractants, or plant growth regulators, containing compounds having three or more carbocyclic rings condensed among themselves, at least one ring not being a six-membered ring
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08—Magnoliopsida [dicotyledons]
    • A01N65/14—Celastraceae [Staff-tree or Bittersweet family], e.g. spindle tree, bittersweet or thunder god vine

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Environmental Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Dentistry (AREA)
  • Zoology (AREA)
  • Agronomy & Crop Science (AREA)
  • Plant Pathology (AREA)
  • Pest Control & Pesticides (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Biotechnology (AREA)
  • Botany (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Ecology (AREA)
  • Forests & Forestry (AREA)
  • Toxicology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention relates to the technical field of citrus pest control, and particularly discloses a botanical preparation for controlling red spiders, which comprises the following raw materials in percentage by mass: 2-10% of berberine, 2-4% of coptisine, 3-10% of capsaicin, 3-5% of tripterygium wilfordii alkaloid, 0.05-0.1% of kaempferol, 0.01-0.03% of beta-sitosterol, 0.02-0.03% of rutin, 0.03-0.05% of luteolin, 4-8% of orange oil, 0.08-0.2% of limonin, 3-6% of chitosan oligosaccharide and 1-3% of mannitol. The botanical preparation for preventing and treating the red spiders, disclosed by the invention, has the advantages of scientific compounding of different botanical substances, good compatibility action, capability of quickly killing citrus red spiders and worm eggs thereof, quick response, long lasting period, capability of avoiding the generation of drug resistance of pests and the like compared with chemical pesticide killing, no chemical hazard, no residue, no environmental pollution, greenness and safety, and has ecological benefits of being friendly to human bodies and environment.

Description

Botanical preparation for preventing and treating red spiders and application thereof
Technical Field
The invention belongs to the technical field of prevention and control of citrus diseases and insect pests, and particularly relates to a botanical preparation for preventing and controlling red spiders and application thereof.
Background
The citrus is an economic fruit, is rich in nutrition, is rich in vitamin C, is delicious in taste, is planted in more than one hundred and forty countries all over the world, and is the first fruit in the world. Citrus is one of the most important economic fruit trees in China, and has a long cultivation history, rich resources and wide distribution in China. During the growth of the citrus, various plant diseases and insect pests can be encountered, if the control is not timely carried out, the yield of the citrus is reduced to different degrees, the fruit quality of the citrus is reduced, and therefore the economic benefit is reduced.
The citrus red spider belongs to the genus Tetranychus of Acarina of Arachnida, and is widely harmful to hosts, such as oranges, loquats, grapes, cherries, peaches, pears and the like, particularly seriously harmful to citrus, mainly harmful to parts of citrus, such as leaves, twigs, young fruits, flower buds and the like, particularly serious damage to the tender leaves and the young tips, the damaged parts fade green firstly, then appear grey-white spots, the leaves lose luster, damaged fruits are grey-white, and a large amount of fallen leaves, fallen flowers, fallen fruits are seriously, tender and withered seriously, so that the yield and the quality of the citrus are greatly influenced. At present, the method for preventing and treating the citrus red mites mainly adopts a chemical acaricide to prevent and treat the citrus red mites, but the citrus red mites have larger propagation coefficient and frequent generation, eggs, young mites, nymphs and adult mites are in four forms on one leaf, and no acaricide can kill the four forms at the same time at present; the resistance to the acaricide is extremely easy to generate, so that the pesticide effect is obviously reduced, meanwhile, the acaricide is largely and repeatedly used due to the shortage of the acaricide and the variety substitution of the acaricide, the generation of the resistance to the citrus red spiders is accelerated, the body wall of the citrus red spiders generating the resistance is thickened, the barrier effect is enhanced, and the speed and the dosage of the common liquid medicine reaching the target are limited; secondly, the toxin-removing metabolism of the citrus red spiders is enhanced, the sensitivity to medicaments is reduced, the pesticide effect is greatly reduced, the citrus red spiders are more and more seriously damaged, and great economic loss is caused to farmers. The chemical agent is adopted to prevent citrus red spiders, and the chemical agent also has short duration, can achieve better effect after being applied, but often explodes again after three or five days; the toxicity of the medicament is high, the medicament residue is serious, the environment is polluted, the growth environment of fruit trees is worsened and the like. Many natural plants contain insecticidal substances. The botanical pesticide with natural phytochemicals as effective components has the advantages of environment friendship, low toxicity, less resistance of pests, etc. The existing botanical pesticide also has the defects that the content of the active ingredients of the existing pesticide is not clear, so that the use effect is influenced; slow drug effect, short effect duration and the like. Therefore, the development of a plant source preparation which is green, has no residue, takes effect quickly and has long-lasting efficacy and is used for preventing and treating red spiders plays an important role in increasing the income of the citrus industry and realizing the sustainable development of the citrus industry.
Disclosure of Invention
The invention aims to provide a botanical preparation for preventing and treating red spiders, which overcomes the defects of short duration, high toxicity, easy generation of drug resistance and the like of chemical agents for preventing and treating red spiders.
In order to realize the purpose, the invention provides a botanical preparation for preventing and treating red spiders, which comprises the following raw materials in percentage by mass: 2-10% of berberine, 2-4% of coptisine, 3-10% of capsaicin, 3-5% of tripterygium wilfordii alkaloid, 0.05-0.1% of kaempferol, 0.01-0.03% of beta-sitosterol, 0.02-0.03% of rutin, 0.03-0.05% of luteolin, 4-8% of orange oil, 0.08-0.2% of limonin, 3-6% of chitosan oligosaccharide and 1-3% of mannitol.
Preferably, the botanical preparation for controlling red spiders comprises the following raw materials in percentage by mass: 8% of berberine, 2% of coptisine, 6% of capsaicin, 4% of tripterygium wilfordii alkaloid, 0.08% of kaempferol, 0.015% of beta-sitosterol, 0.025% of rutin, 0.04% of luteolin, 6% of orange oil, 0.12% of limonin, 5% of chitosan oligosaccharide and 1.5% of mannitol.
Preferably, the botanical preparation for preventing and treating red spiders further comprises 3-6% of an auxiliary agent, wherein the auxiliary agent is formed by mixing glycerol and plant type polyoxyethylene ether according to a weight ratio of 1: 2-3.
A preparation method of a plant source preparation for preventing and treating red spiders comprises the following steps:
(1) homogenizing and stirring coptisine, capsaicin, tripterygium wilfordii alkaloid, kaempferol, beta-sitosterol, rutin, limonin, orange oil and an auxiliary agent at 1000-2000 r/min for 15-30 min to obtain a mixed substance;
(2) adding berberine, luteolin, chitosan oligosaccharide and mannitol into water, stirring and dissolving to obtain a mixed solution;
(3) and (3) adding the mixed solution obtained in the step (2) into the mixed substance obtained in the step (1), supplementing 100% of water, and then stirring at a high speed to obtain the plant source preparation for controlling the red spiders.
The plant source preparation for preventing and treating red spiders is applied to preventing and treating citrus red spiders.
Preferably, in the application, the plant source preparation is applied to citrus trees suffering from red spider insect pests, the plant source preparation is diluted by 500-600 times of water to obtain a liquid medicine, and the liquid medicine is sprayed on the leaves of the citrus trees.
Preferably, in the application, the dosage of the spraying liquid medicine is 35-50L/mu.
The inventor obtains the plant source preparation for preventing and treating red spider through previous repeated screening and tests, wherein berberine exists in berberidaceae, papaveraceae, ranunculaceae, rutaceae, menispermaceae and rhamnaceae plants, can be extracted from coptis chinensis, phellodendron amurense, barberry and other plants, and has rich plant resources and low market price; berberine has effects of resisting arrhythmia, lowering blood pressure, blood lipid and blood sugar, inhibiting inflammation, bacteria and virus infection, inhibiting bacteria and inflammation for many fungi and bacterial diseases, even for livestock, preventing and treating aphids and mites, and weeding many weeds;
coptisine is a natural protoberberine type alkaloid quaternary ammonium salt, exists in various plants of Ranunculaceae and Papaveraceae, is rich in resources and wide in pharmacological action, has antifungal activity, changes cell membrane permeability, destroys nutrient substances and excrement of cell membranes, diffuses into cells, and further combines phospholipid of the cell membranes and cell nucleuses to cause the disappearance of organelles and damage to the structures and functions of the cells;
capsaicin, also called capsaicin, is present in capsicum, a solanaceous plant, and consists essentially of 3 analogs: the medicine consists of nordihydrocapsaicin, capsaicin and dihydrocapsaicin, and the capsaicin has the effects of sterilization and disinfection and can promote the absorption of the outer tissues of the red spiders to the liquid medicine;
tripterygium wilfordii alkaloid exists in Tripterygium wilfordii plant, and is monomer mixture containing at least three different alkaloids of Tripterygium wilfordii, Tripterygium wilfordii alkaloid and Tripterygium wilfordii alkaloid by chemical extraction and separation, and has antiinflammatory, immunosuppressive, antitumor, fertility inhibiting, and pesticidal effects;
kaempferol, flavonoid secondary metabolite, is present in various plants, and is derived from folium Camelliae sinensis, Cauliflower, fructus Coryli Heterophyllae, propolis,ShaddockOther green plant extracts; it has anticancer, antiinflammatory, antioxidant, antibacterial, and antiviral effects, and has effects in inhibiting in vivo enzyme activity of insect, and influencing normal metabolism of insect;
beta-sitosterol, a wide variety of occurring in natureVegetable oil、NutThe plant seeds are also present in certain plant medicines and are used in medical raw materials and health-care foods; beta-sitosterol obviously changes the protein content in the insect body and the interconversion of carbohydrate-fat-protein, thus influencing the normal development of the insect;
rutin, also known as rutin and vitamin P, is a natural flavonoid glycoside, widely exists in plants such as myrcia, tobacco leaves, orange peels, tomatoes and the like, has the effects of resisting inflammation, oxidation, allergy, virus and the like, and can inhibit the activity of important enzymes in insects;
luteolin, a natural flavonoid compound, exists in various plants such as dracocephalum heterophyllum benth, capsicum, wild chrysanthemum flower, honeysuckle flower, perilla frutescens and the like, can be separated from various natural medicinal materials and fruits of vegetables, has various pharmacological activities such as diminishing inflammation, resisting allergy, reducing uric acid, resisting tumor, bacteria and viruses and the like, and can influence the normal physiological activity of insects together with metal ions such as calcium, iron and the like;
limonin exists in citrus fruits and has certain anticancer and antiviral effects and other beneficial biological activities;
the chitosan oligosaccharide is the only cationic basic amino oligosaccharide with positive charge in nature, and is a micromolecular oligosaccharide with amino groups degraded from chitosan of shrimp and crab shells, the chitosan oligosaccharide contains charges, so that the gradient difference of an oxidation-reduction field formed on two sides of a biological membrane can be enhanced to be larger, the permeation of liquid medicine is facilitated, the speed of the liquid medicine reaching a target is increased, meanwhile, the chitosan oligosaccharide has more remarkable biological activity, the opening or closing of an ion channel for signal conduction of pests can be effectively regulated, and the conformation of carrier protein changes physiological growth and metabolic activity, so that the purpose of killing pests is achieved;
mannitol is a sugar alcohol, has sweet taste similar to sucrose, has the effects of attracting pests to suck liquid medicine, can regulate cell osmotic pressure and is beneficial to the absorption of the pests to the medicine; the orange oil is volatile oil extracted from plants, and has effects of improving penetration and spreadability of medicinal liquid;
according to the invention, multiple (plant source substances) Chinese herbal medicines are compounded, so that the control effect on plant diseases and insect pests can be improved according to the synergistic effect of different active ingredients in the formula, the quick action and the lasting effect are improved, the pests are prevented from generating drug resistance, and the pesticide has the advantages of low toxicity, high efficiency, strong selectivity and the like.
Compared with the prior art, the invention has the following beneficial effects:
1. the botanical preparation for preventing and treating the red spiders is prepared by scientifically compounding various botanical substances, has good compatibility, can quickly kill the citrus red spiders and eggs thereof, has the advantages of quick response, long lasting period, avoidance of generation of drug resistance of pests and the like compared with chemical pesticide killing, has no phytotoxicity, no pesticide residue, no environmental pollution, greenness and safety, and has ecological benefits of being friendly to human bodies and environment.
2. The botanical preparation for preventing and treating red spiders, disclosed by the invention, utilizes different prevention and treatment action mechanisms and synergistic effects of various plant bioactive components according to the characteristics and action mechanisms of various natural plant active components, destroys a digestive system, interferes nerve conduction of pests, inhibits detoxification enzymes and endocrine to inhibit the growth of pest populations, and is beneficial to enhancing the prevention and treatment effect on citrus red spiders.
3. The botanical preparation for preventing and treating red spiders, disclosed by the invention, has the advantages of easiness in obtaining raw materials, simplicity in preparation, easiness in operation of a using method, thorough prevention and treatment effect on red spiders in oranges, almost 100% prevention and treatment rate, capability of sustaining for 45-55 days, no toxic action on oranges, capability of providing organic nutrition for oranges and promotion of bright green of orange leaf surfaces.
Detailed Description
The following detailed description of specific embodiments of the invention is provided, but it should be understood that the scope of the invention is not limited to the specific embodiments.
Example 1
A botanical preparation for preventing and treating red spiders comprises the following raw materials in percentage by mass: 8% of berberine, 2% of coptisine, 6% of capsaicin, 4% of tripterygium wilfordii alkaloid, 0.08% of kaempferol, 0.015% of beta-sitosterol, 0.025% of rutin, 0.04% of luteolin, 6% of orange oil, 0.12% of limonin, 5% of chitosan oligosaccharide, 2% of mannitol, 1.5% of glycerol, 4% of plant type polyoxyethylene ether (NSF-10E) and the balance of water.
The embodiment also provides a preparation method of the plant source preparation for controlling red spiders, which comprises the following steps:
(1) weighing the raw materials according to the weight fraction of the raw materials;
(2) homogenizing coptisine, capsaicin, Tripterygium wilfordii alkaloid, kaempferol, beta-sitosterol, rutin, limonin, orange oil, glycerol and plant type polyoxyethylene ether at 1500r/min, stirring for 30min, and mixing to obtain mixed substance;
(3) adding berberine, luteolin, chitosan oligosaccharide and mannitol into water, stirring and dissolving to obtain a mixed solution;
(4) and (3) adding the mixed solution obtained in the step (3) into the mixed substance obtained in the step (2), and homogenizing and emulsifying at a high speed of 4000r/min to obtain the plant source preparation for preventing and treating the red spider.
Example 2
A botanical preparation for preventing and treating red spiders comprises the following raw materials in percentage by mass: 5% of berberine, 3% of coptisine, 9% of capsaicin, 3% of tripterygium wilfordii alkaloid, 0.05% of kaempferol, 0.02% of beta-sitosterol, 0.02% of rutin, 0.04% of luteolin, 7% of orange oil, 0.15% of limonin, 4% of chitosan oligosaccharide, 2% of mannitol, 1.5% of glycerol, 4% of plant type polyoxyethylene ether (NSF-10E) and the balance of water.
Example 3
A botanical preparation for preventing and treating red spiders comprises the following raw materials in percentage by mass: 7% of berberine, 3.5% of coptisine, 7% of capsaicin, 3.5% of tripterygium wilfordii alkaloid, 0.07% of kaempferol, 0.02% of beta-sitosterol, 0.028% of rutin, 0.03% of luteolin, 6% of orange oil, 0.1% of limonin, 5% of chitosan oligosaccharide, 1.5% of mannitol, 1% of glycerol, 3% of plant type polyoxyethylene ether (NSF-10E) and the balance of water.
Application example
The plant source preparation for controlling red spiders in the embodiment 1-3 is applied to red spider control in a citrus planting process.
The red spider prevention and control test is carried out in the citrus phytolaccatus planting base, the citrus tree age of the phytolaccatus is 4 years, the plant spacing of the citrus trees is 2m, the row spacing is 3m, the cultivation period, the growth period and the density of each treatment are consistent, intertillage weeding, water and fertilizer and the like are managed according to the conventional method, and no other medicament is used in the test period. During the test, the red spiders in the field are in the advanced adult mite stage, the leaves are investigated by using a handheld magnifier in the test, and the occurrence conditions of the red spiders in the test area are basically consistent.
The test is carried out by setting 5 treatment groups which are respectively an example 1-3 group, a pesticide comparison group and a clear water comparison group, wherein each treatment group is provided with 3 repetitions, the treatment groups are arranged in random blocks, and each cell is 30cm2And protective rows are arranged around the protective rows. The method comprises the following steps of spraying liquid medicine on citrus trees by using a sprayer, wherein the liquid medicine is not dropped, the plant source preparations of examples 1-3 are respectively diluted into liquid medicine by using 500 times of water, and the liquid medicine is sprayed by using a knapsack sprayer, wherein the liquid medicine amount per mu is 40L; the pesticide control group is that 40% dicofol missible oil is diluted 1000 times, the application amount of the 40% dicofol missible oil is 40L, and the clear water control group adopts clear water spraying.
Two citrus trees are taken in each cell, lateral branches are marked in east, west, south, north and middle 5 directions of the trees, the number of red spiders on the front and back sides of 20 leaves is investigated totally, and all live mites are directly observed and recorded by a hand-held magnifier. The number of the base of the study before administration was 800 or more, and 8 studies were conducted 1, 3, 7, 14, 20, 30, 40, and 55 days after administration. According to the base number of red spiders before the medicine and the number of the survival red spiders in each day after the medicine, the population reduction rate and the prevention and treatment effect are calculated.
The oral cavity decline rate is (number of live red spiders before the drug-number of live red spiders after the drug)/number of live red spiders before the drug × 100%;
the control effect is (the oral cavity decline rate of the treated area-the oral cavity decline rate of the control area)/(100-the oral cavity decline rate of the control area) × 100%.
The table 1 and the table 2 show the population reduction rate and the control effect of each treatment group after the pesticide is applied, and the plant source preparation can effectively kill red spiders and worm eggs and has quick-acting performance, the population reduction rate is more than 99% in the first day after the pesticide is applied, the population reduction rate is still more than 99% in 14 days after the pesticide is applied, the control effect is more than 98% in 20 days after the pesticide is applied, the control effect is improved by 100% compared with that of a pesticide comparison group, and the control effect can be continued for 45-55 days, and has long-acting performance. Compared with the control group and the control group, the leaf surfaces of the plants of the groups of examples 1-3 after the application of the pesticide are brighter green.
TABLE 1 reduction of population by treatment groups
Figure BDA0003351976240000071
TABLE 2 controlling Effect of the treatment groups after application of the drugs
Figure BDA0003351976240000072
The foregoing descriptions of specific exemplary embodiments of the present invention have been presented for purposes of illustration and description. It is not intended to limit the invention to the precise form disclosed, and obviously many modifications and variations are possible in light of the above teaching. The exemplary embodiments were chosen and described in order to explain certain principles of the invention and its practical application to enable one skilled in the art to make and use various exemplary embodiments of the invention and various alternatives and modifications as are suited to the particular use contemplated. It is intended that the scope of the invention be defined by the claims and their equivalents.

Claims (7)

1. The botanical preparation for preventing and treating the red spiders is characterized by comprising the following raw materials in percentage by mass: 2-10% of berberine, 2-4% of coptisine, 3-10% of capsaicin, 3-5% of tripterygium wilfordii alkaloid, 0.05-0.1% of kaempferol, 0.01-0.03% of beta-sitosterol, 0.02-0.03% of rutin, 0.03-0.05% of luteolin, 4-8% of orange oil, 0.08-0.2% of limonin, 3-6% of chitosan oligosaccharide and 1-3% of mannitol.
2. The plant source preparation for controlling red spiders according to claim 1, which is characterized by comprising the following raw materials in percentage by mass: 8% of berberine, 2% of coptisine, 6% of capsaicin, 4% of tripterygium wilfordii alkaloid, 0.08% of kaempferol, 0.015% of beta-sitosterol, 0.025% of rutin, 0.04% of luteolin, 6% of orange oil, 0.12% of limonin, 5% of chitosan oligosaccharide and 2% of mannitol.
3. The botanical preparation for controlling red spiders according to claim 1, further comprising 3-6% of an auxiliary agent, wherein the auxiliary agent is a mixture of glycerol and plant type polyoxyethylene ether in a weight ratio of 1: 2-3.
4. A method for preparing a plant-derived preparation for controlling red spiders according to claim 3, comprising the steps of:
(1) homogenizing and stirring coptisine, capsaicin, tripterygium wilfordii alkaloid, kaempferol, beta-sitosterol, rutin, limonin, orange oil and an auxiliary agent at 1000-2000 r/min for 15-30 min to obtain a mixed substance;
(2) adding berberine, luteolin, chitosan oligosaccharide and mannitol into water, stirring and dissolving to obtain a mixed solution;
(3) and (3) adding the mixed solution obtained in the step (2) into the mixed substance obtained in the step (1), supplementing 100% of water, and then stirring at a high speed to obtain the plant source preparation for controlling the red spiders.
5. The application of the plant source preparation for controlling red spiders according to any one of claims 1 to 3 in controlling citrus red spiders.
6. The application of claim 5, wherein the plant source preparation is applied to citrus trees suffering from red spider insect pests, the plant source preparation is diluted with 500-600 times of water to obtain a liquid medicine, and the liquid medicine is sprayed on the leaf surfaces of the citrus trees.
7. The application of claim 6, wherein the spraying amount of the liquid medicine is 35-50L/mu.
CN202111339597.9A 2021-11-12 2021-11-12 Botanical preparation for preventing and treating red spiders and application thereof Pending CN113966745A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202111339597.9A CN113966745A (en) 2021-11-12 2021-11-12 Botanical preparation for preventing and treating red spiders and application thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202111339597.9A CN113966745A (en) 2021-11-12 2021-11-12 Botanical preparation for preventing and treating red spiders and application thereof

Publications (1)

Publication Number Publication Date
CN113966745A true CN113966745A (en) 2022-01-25

Family

ID=79589742

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202111339597.9A Pending CN113966745A (en) 2021-11-12 2021-11-12 Botanical preparation for preventing and treating red spiders and application thereof

Country Status (1)

Country Link
CN (1) CN113966745A (en)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101940229A (en) * 2010-08-05 2011-01-12 宁夏农林科学院 Berberine plant source insecticide and preparation method thereof
CN104115877A (en) * 2014-06-27 2014-10-29 广西平乐农药厂 Biological pesticide composition
CN105123777A (en) * 2015-08-26 2015-12-09 青岛文创科技有限公司 Botanical insecticide
CN105532755A (en) * 2015-12-16 2016-05-04 罗永城 Novel plant source insecticide for fruit trees, preparation method and application thereof
CN105994432A (en) * 2016-05-26 2016-10-12 长沙理工大学 Botanical spider mite insecticide for organic plums and using method thereof

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101940229A (en) * 2010-08-05 2011-01-12 宁夏农林科学院 Berberine plant source insecticide and preparation method thereof
CN104115877A (en) * 2014-06-27 2014-10-29 广西平乐农药厂 Biological pesticide composition
CN105123777A (en) * 2015-08-26 2015-12-09 青岛文创科技有限公司 Botanical insecticide
CN105532755A (en) * 2015-12-16 2016-05-04 罗永城 Novel plant source insecticide for fruit trees, preparation method and application thereof
CN105994432A (en) * 2016-05-26 2016-10-12 长沙理工大学 Botanical spider mite insecticide for organic plums and using method thereof

Similar Documents

Publication Publication Date Title
CN102578164B (en) Compounded botanical synergistic pesticide containing sophocarpidine and macleaya alkaloids and preparation method of compounded botanical synergistic pesticide
CN104855442B (en) For preventing and treating plant pathogeny line insect medicine fertilizer and application process
CN100382707C (en) A botanical pesticide
Kumar et al. Screening of selected biological activities of Artocarpus lakoocha Roxb (Moraceae) fruit pericarp
CN104322572B (en) A kind of plant insecticide
CN103535389A (en) Plant source fruit tree insecticide
CN102283260A (en) Biologic bactericide prepared from plant sources
CN106467433A (en) Fruit tree diseases prevention promotees bud and keeps a full stand of seedings accelerator and its application
CN111066831A (en) Chinese herbal medicine source pesticide
Haq et al. Phytochemical and biological evaluation of defatted seeds of Jatropha curcas
CN108477241A (en) A kind of Queensland nut pest control method
KR100852398B1 (en) Composition for controlling noxious animals in horticultural crops and using method thereof
CN105407725B (en) The composition and method of selectivity control invasive species
Vinayaka et al. Studies on in vitro antioxidant, antibacterial and insecticidal activity of methanolic extract of Abrus pulchellus Wall (Fabaceae)
CN104920487B (en) A kind of Chinese medicinal material extract and preparation method thereof and the method using Chinese medicinal material extract preventing and treating wheat aphid
CN104886168A (en) Biopesticide for preventing and treating tomato bacterial wilt and preparation method thereof
Kushwaha et al. Plant Anacyclus pyrethrum-A review
Rekha et al. Cultivation prospects of endangered species Celastrus paniculatus Willd.
CN113966745A (en) Botanical preparation for preventing and treating red spiders and application thereof
Rajani et al. A review on medicinal uses, pharmacology and phytochemistry of Aristolochia tagala Cham. An endangered medicinal plant
CN113367164A (en) Method for extracting castor-oil plant insecticidal active substance and combined application of castor-oil plant insecticidal active substance and microbial source insecticide
CN103918705B (en) Pesticidal combination containing spiral shell worm ethyl ester and gamma cyhalothrin and application thereof
CN113767901A (en) Application of thymol in prevention and treatment of peanut southern blight
CN103444794A (en) Bactericide prepared from plant extract liquid
CN106495862A (en) A kind of composite pesticide for apple tree cultivation and preparation method thereof

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
RJ01 Rejection of invention patent application after publication
RJ01 Rejection of invention patent application after publication

Application publication date: 20220125