CN113956966B - Bioreactor gas flow testing method - Google Patents

Bioreactor gas flow testing method Download PDF

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Publication number
CN113956966B
CN113956966B CN202111236225.3A CN202111236225A CN113956966B CN 113956966 B CN113956966 B CN 113956966B CN 202111236225 A CN202111236225 A CN 202111236225A CN 113956966 B CN113956966 B CN 113956966B
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pipe
gas
detection
block
control piece
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CN113956966A (en
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陈超
卜兴
李达源
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Shanghai Zhihe Biotechnology Co ltd
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Shanghai Zhihe Biotechnology Co ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/48—Holding appliances; Racks; Supports
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/50—Means for positioning or orientating the apparatus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/20—Degassing; Venting; Bubble traps
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • C12M37/04—Seals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Clinical Laboratory Science (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
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Abstract

The application relates to a bioreactor gas flow testing method, which comprises the following steps of S1: connecting an exhaust pipe to the bioreactor; s2: the exhaust pipe is provided with a first control piece, a second control piece and a detection pipe, and the detection pipe is provided with a detection unit and a third control piece; s3: when detection is needed, the first control piece, the second control piece and the third control piece are adjusted, so that gas can flow in the exhaust pipe and cannot enter the detection pipe; s4: after a period of time, the third control piece and the second control piece are adjusted, so that gas can enter the detection pipe through the exhaust pipe and cannot circulate in the exhaust pipe; detecting the gas by a detection unit on the detection tube after a period of time to obtain a group of values; finally, the second control piece and the third control piece are regulated to enable the gas to flow in the exhaust pipe and not enter the detection pipe; s5: and S3, processing the plurality of groups of values, and finally calculating the final value.

Description

Bioreactor gas flow testing method
Technical Field
The application relates to the technical field of gas detection, in particular to a method for testing gas flow of a bioreactor.
Background
Bioreactor refers to a reaction system that utilizes naturally occurring microorganisms or microorganisms with specific degradability to inoculate a liquid or solid phase. After the bioreactor generates the gas, the gas generated by the bioreactor needs to be detected.
At present, the conventional detection is that an exhaust pipe is directly connected to the bioreactor, a control valve is arranged at one end of the exhaust pipe, which is close to the bioreactor, and a gas flowmeter is arranged on the exhaust pipe. And the control valve is opened, gas generated by the bioreactor enters the exhaust pipe, and the gas flow meter detects the flow of the gas entering the exhaust pipe.
In carrying out the present application, the inventors have found that at least the following problems exist in this technology: the gas that bioreactor produced can enter into the blast pipe when opening the control, and more and also can produce certain impact force at original gas, when the impact force acted on gas flowmeter, can cause certain damage to gas flowmeter, and gas flowmeter damages the back, thereby the manual work is difficult to observe, thereby leads to detecting inaccurately when continuing to detect gas flow with the gas flowmeter of damage.
Disclosure of Invention
In order to improve detection accuracy, the application provides a bioreactor gas flow testing method.
The method for testing the gas flow of the bioreactor adopts the following technical scheme:
a bioreactor gas flow test method comprising the steps of: s1: firstly, connecting an exhaust pipe to a bioreactor; s2: the exhaust pipe is sequentially provided with a first control piece and a second control piece along the gas flowing direction, a detection pipe is arranged on the exhaust pipe between the first control piece and the second control piece, a detection unit is arranged on the detection pipe, and a third control piece is arranged on the detection pipe between the detection unit and the exhaust pipe; s3: when detection is needed, the third control piece is adjusted, so that the third control piece seals a channel of the detection tube, and gas cannot enter the detection tube; adjusting the first control member and the second control member to enable the gas to flow in the exhaust pipe; s4: after a period of time, the third control piece is adjusted first, so that gas can enter the detection pipe through the exhaust pipe, and then the second control piece is adjusted, so that the second control piece seals a channel of the exhaust pipe; detecting the gas by a detection unit on the detection tube after a period of time to obtain a group of values; finally, the second control piece is regulated to enable the gas to flow in the exhaust pipe again, and the third control piece is regulated to enable the third control piece to seal the channel of the detection pipe; s5: and S4, processing the plurality of groups of values, and finally calculating the final value.
By adopting the technical scheme, when the flow of the gas generated in the bioreactor is required to be detected, the third control piece seals the channel of the detection pipe, and then the first control piece and the second control piece are adjusted to enable the gas to flow in the exhaust pipe; after the gas flows in the exhaust pipe for a period of time, the third control piece is firstly adjusted to enable the gas to flow in the exhaust pipe again, then the second control piece is adjusted to enable the second control piece to seal a channel of the exhaust pipe, a detection unit on the detection pipe can detect the gas flowing in the detection pipe stably, and then a group of values are obtained; then repeatedly detecting by using a gas flowmeter, and finally detecting a plurality of groups of data; the set testing method can improve the detection accuracy.
Optionally, the detection unit includes a connection pipe, a gas flowmeter, a first check valve, a fourth control piece and a fifth control piece, the connection pipe is arranged at one side of the detection pipe, and two ends of the connection pipe are connected with the detection pipe; the fourth control piece is arranged on the detection tube between the two ends of the connecting tube; the fifth control piece is arranged at one end of the connecting pipe close to the exhaust pipe, the first one-way valve is arranged at the other end of the connecting pipe, and the gas flowmeter is arranged on the connecting pipe between the fifth control piece and the first one-way valve.
By adopting the technical scheme, when the gas enters the detection tube, the fifth control piece seals the passage of the connecting tube, and the gas enters the detection tube; after a period of time, the fifth control element is adjusted to enable the gas to enter the connecting pipe; and adjusting the fourth control piece to enable the fourth control piece to seal the channel of the detection pipe, and after a period of time, measuring the data measured by the gas flow meter on the connecting pipe to be the required data.
Optionally, the detecting unit is provided with a plurality of, and a plurality of detecting units in the range of the gas flowmeter, along the one end that keeps away from the blast pipe diminishes gradually.
By adopting the technical scheme, when the data detected by the gas flow meters close to the exhaust pipe is smaller, the gas is detected by more gas flow meters, and the average value of the data detected by the plurality of gas flow meters is taken; when the data detected by the gas flow meter close to the exhaust pipe is larger, the gas can be detected by a small amount of gas flow meters, then the subsequent gas flow meters can not detect the gas any more, and the average value of the data detected by the gas flow meters is taken.
Optionally, the detecting tube upper air outlet assembly comprises a first air outlet tube, a second air outlet tube, a third air outlet tube, a second one-way valve and a sixth control member, wherein a plurality of first air outlet tubes are arranged, and the first air outlet tubes are arranged on the detecting tubes between two adjacent detecting units; the second air outlet pipe is arranged at one side of the detection pipe, and a plurality of first air outlet pipes are connected with the second air outlet pipe; the third air outlet pipe is arranged at one end of the detection pipe far away from the exhaust pipe, and is connected with the second air outlet pipe; the first air outlet pipe and the third air outlet pipe are respectively provided with the second one-way valve and the sixth control piece.
By adopting the technical scheme, when the gas is detected by the gas flow meters of a certain number of detection units, a sixth control piece on the first gas outlet pipe between the last used detection unit and the first unused detection unit is opened, and other sixth control pieces seal the channels of other first gas outlet pipes, so that the gas can enter the second gas outlet pipe through the first gas outlet pipe and finally is discharged; when the gas is carried out by the gas flow meters in all the detection units, all the channels of the first connecting pipes are sealed by the sixth control piece, then the gas enters the second gas outlet pipe through the third gas outlet pipe, and finally is discharged.
Optionally, the detection unit further comprises a supporting device, the supporting device comprises a fixing frame, a supporting block, a placing block and a first adjusting component, and the connecting pipe is arranged on the fixing frame; the supporting block is arranged on the supporting frame; the two placing blocks are arranged, the two placing blocks are arranged on the supporting block, and the gas flowmeter is arranged on the placing blocks; the first adjusting component comprises a first motor and a first screw rod, the first motor is arranged on the supporting frame, the first screw rod is connected with an output shaft of the first motor, and the first screw rod penetrates through the supporting block and is connected with the supporting block.
By adopting the technical scheme, the arranged fixing frame supports the detection tube; the placing block on the supporting block supports the gas flowmeter. When the gas flowmeter is damaged, the gas flowmeter needs to be replaced, or the detection range needs to be adjusted by replacing the gas flowmeter, a new gas flowmeter is placed on a placement block, then the output shaft of the first motor drives the first screw to rotate, and the first screw drives the support block to move on the support frame, so that the new gas flowmeter is replaced by the gas flowmeter needing to be replaced.
Optionally, the supporting block is provided with a clamping mechanism, the clamping mechanism comprises a fixed block, a connecting rod, a clamping block and a second adjusting component, the fixed block is arranged on the supporting block, and the placing block is arranged on the fixed block; the connecting rods are slidably arranged on the fixed blocks on two sides of the placing block, the clamping blocks are arranged on the connecting rods, and the two clamping blocks are abutted against the gas flowmeter; the second adjusting component comprises a second motor and a bidirectional screw, and the second motor is arranged on the fixed block; the bidirectional screw rod is arranged on the output shaft of the second motor, the bidirectional screw rod penetrates through the two connecting rods, and the two connecting rods are respectively in threaded connection with the two ends of the bidirectional screw rod.
By adopting the technical scheme, after the gas flowmeter is arranged on the placing block, the output shaft of the second motor drives the bidirectional screw rod to rotate, the bidirectional screw rod drives the two connecting rods to move in opposite directions, and the clamping blocks on the two connecting rods are propped against the gas flowmeter; the fixture that sets up can improve the stability of gas flowmeter on placing the piece.
Optionally, an adjusting mechanism is arranged on the supporting block, the adjusting mechanism comprises an adjusting block, a second screw and a third motor, the adjusting block is slidingly arranged on the supporting block, and the placing block is arranged on the adjusting block; the second screw rod passes through the adjusting block and is in threaded connection with the adjusting block; the third motor is arranged on the supporting block, and an output shaft of the third motor is connected with the second screw.
Through adopting above-mentioned technical scheme, the third motor drives the second screw rod and rotates, and the second screw rod rotates and will drive the adjusting block motion, and the adjusting block drives gas flowmeter motion to reduce manual adjustment, improve work efficiency.
Optionally, a positioning mechanism is arranged on the supporting block, the positioning mechanism comprises a positioning plate, a rack, a gear and a fourth motor, the positioning plate is slidably arranged on the supporting block, and the gas flowmeter abuts against the positioning plate; the rack is arranged on the positioning plate; the fourth motor is arranged on the supporting block, and the gear is arranged on an output shaft of the fourth motor and meshed with the rack.
By adopting the technical scheme, the output shaft of the fourth motor drives the gear to rotate, and the rack meshed with the gear drives the positioning plate to move, so that part of the positioning plate extends out of the supporting block; when the gas flowmeter abuts against the positioning plate, the gas flowmeter is at an optimal position, so that the gas flowmeter can be conveniently replaced; the positioning mechanism can position the gas flowmeter, so that the time of manual adjustment is reduced, and the efficiency is improved.
In summary, the present application includes the following beneficial technical effects:
1. the set testing method can improve the detection accuracy;
2. the positioning mechanism can position the gas flowmeter, so that the time of manual adjustment is reduced, and the efficiency is improved.
Drawings
FIG. 1 is a schematic view of a structure in which a detection pipe is connected to an exhaust pipe according to an embodiment of the present application;
FIG. 2 is a schematic structural view of a first adjusting component according to an embodiment of the present application;
FIG. 3 is a schematic structural diagram of an adjusting mechanism according to an embodiment of the present disclosure;
fig. 4 is a schematic structural diagram of a positioning mechanism in an embodiment of the present application.
Reference numerals: 11. a bioreactor; 12. an exhaust pipe; 13. a first control member; 14. a second control member; 15. a detection tube; 16. a third control member; 17. a mounting frame; 18. a connecting frame; 2. a detection unit; 21. a connecting pipe; 22. a gas flow meter; 23. a first one-way valve; 24. a fourth control member; 25. a fifth control member; 3. an air outlet assembly; 31. a first air outlet pipe; 32. a second air outlet pipe; 33. a third air outlet pipe; 34. a second one-way valve; 35. a sixth control member; 4. a support device; 41. a fixing frame; 42. a support frame; 421. a first chute; 43. a support block; 431. a second chute; 44. placing a block; 45. a first adjustment assembly; 451. a first motor; 452. a first screw; 5. a clamping mechanism; 51. a fixed block; 511. a third chute; 52. a connecting rod; 53. a clamping block; 54. a second adjustment assembly; 541. a second motor; 542. a bidirectional screw; 6. an adjusting mechanism; 61. adjusting the block; 62. a second screw; 63. a third motor; 7. a positioning mechanism; 71. a positioning plate; 72. a rack; 73. a gear; 74. and a fourth motor.
Detailed Description
The present application is described in further detail below in conjunction with figures 1-4. In the embodiment, the first control member 13, the second control member 14, the third control member 16, the fourth control member 24, the fifth control member 25 and the sixth control member 35 are control valves.
The embodiment of the application discloses a bioreactor gas flow testing method.
Referring to fig. 1 and 2, the bioreactor gas flow test method comprises the steps of:
s1: the exhaust pipe 12 is first connected to the bioreactor 11.
S2: the exhaust pipe 12 is provided with a first control member 13 and a second control member 14 in the gas flow direction, a detection pipe 15 is installed on the exhaust pipe 12 between the first control member 13 and the second control member 14, a plurality of groups of detection units 2 are provided on the detection pipe 15, and a third control member 16 is installed on the detection pipe 15 between the detection unit 2 closest to the exhaust pipe 12 and the exhaust pipe 12.
The detection unit 2 comprises a connecting pipe 21 arranged at one side of the detection pipe 15, both ends of the connecting pipe 21 are connected to the detection pipe 15, one end of the connecting pipe 21, which is close to the exhaust pipe 12, is connected with a fifth control piece 25, and one end of the connecting pipe 21, which is far away from the fifth control piece 25, is connected with a first one-way valve 23; a gas flow meter 22 is connected to the connection pipe 21 between the fifth connection piece and the first one-way valve 23. A fourth control member 24 is connected to the detection tube 15 between the two ends of the connection tube 21. And the range of the gas flow meter 22 in the detection unit 2 becomes gradually smaller toward the end away from the exhaust pipe 12.
S3: when detection is needed, the third control piece 16 seals the channel of the detection pipe 15, and then the first control piece 13 and the second control piece 14 are adjusted to enable gas to flow in the exhaust pipe 12; the gas does not enter the detection tube 15.
S4: after the gas flows in the exhaust pipe 12 for a period of time, the third control member 16 is adjusted so that the gas can enter the detection pipe 15, and then the second control member 14 is adjusted so that the second control member 14 seals the passage of the exhaust pipe 12.
When the gas enters the detection tube 15, the fifth control member 25 seals the passage of the connection tube 21, and the gas flows in the detection tube 15; after a period of time, the fifth control 25 is adjusted so that gas can enter the connecting tube 21; the fourth control member 24 is adjusted so that the fourth control member 24 seals the passage of the detection tube 15, and after a certain period of time, the gas flow meter 22 on the connection tube 21 measures data.
When the data detected by the gas flow meters 22 close to the exhaust pipe 12 is smaller, the gas is detected by the gas flow meters 22 with a relatively large number, and the data measured by the plurality of gas flow meters 22 are averaged; when the data detected by the gas flow meter 22 near the exhaust pipe 12 is large, the gas will pass through a small amount of the gas flow meter 22 for detection, then the subsequent gas flow meter 22 will not detect the gas any more, and the average value of the data detected by the gas flow meter 22 is taken.
S5: and S4, processing the plurality of groups of values, and finally calculating the final value.
Referring to fig. 1 and 2, an air outlet assembly 3 is arranged on the detection tube 15, one end of the detection tube 15 far away from the exhaust tube 12 is connected with a third air outlet tube 33, and the third air outlet tube 33 is sequentially connected with a sixth control member 35 and a second one-way valve 34 along the direction far away from the detection tube 15; the connecting frame 18 is placed to the side that detects pipe 15 kept away from connecting pipe 21, has placed second outlet duct 32 on the connecting frame 18, and the one end that the third outlet duct 33 kept away from detecting pipe 15 is connected with second outlet duct 32.
The detection tube 15 between two adjacent detection units 2 is connected with a first air outlet tube 31, the first air outlet tube 31 is sequentially connected with a sixth control member 35 and a second one-way valve 34 along the direction away from the detection tube 15, and one end of the first air outlet tube 31 away from the detection tube 15 is connected with a second air outlet tube 32.
When the gas is carried out by the gas flow meters 22 in all the detecting units 2, all the passages of the first connecting pipes 21 are sealed by the sixth control member 35, and then the gas enters the second gas outlet pipe 32 through the third gas outlet pipe 33 and finally is discharged.
When the gas is detected by the gas flow meters 22 of a certain number of the detecting units 2, the sixth control member 35 on the first gas outlet pipe 31 between the last used detecting unit 2 and the first unused detecting unit 2 is opened, and the other sixth control member 35 seals the passage of the other first gas outlet pipe 31, so that the gas can enter the second gas outlet pipe 32 through the first gas outlet pipe 31 and finally be discharged.
Referring to fig. 1 and 3, a mounting frame 17 is provided at one side of the exhaust pipe 12, and the mounting frame 17 supports the detection pipe 15.
The exhaust pipe 12 is provided with a support device 4 on one side, and the support device 4 includes a fixing frame 41 for supporting the connection pipe 21.
A support frame 42 is arranged on one side of the fixing frame 41 far away from the detection tube 15, a first sliding groove 421 is formed in the support frame 42, a support block 43 is slidably connected in the first sliding groove 421, a first adjusting component 45 is arranged on the support frame 42, the first adjusting component 45 comprises a first screw 452 rotatably connected in the first sliding groove 421, and the first screw 452 penetrates through the support block 43 and is in threaded connection with the support block 43; the support frame 42 is fixedly connected with a first motor 451, and an output shaft of the first motor 451 is connected with one end of a first screw 452.
Referring to fig. 2 and 3, two adjusting mechanisms 6 are disposed on the supporting block 43, a second sliding groove 431 is formed on a side, away from the supporting frame 42, of the supporting block 43, and an axis of the second sliding groove 431 is perpendicular to an axis of the first sliding groove 421.
The adjusting mechanism 6 comprises an adjusting block 61 which is glidingly connected in the second chute 431; the second sliding groove 431 is rotatably connected with a second screw 62, and a second bolt passes through the adjusting block 61 and is in threaded connection with the adjusting block 61; the third motor 63 is fixedly connected to the supporting block 43, and an output shaft of the third motor 63 is connected with one end of the second screw 62.
The clamping mechanism 5 is arranged on the adjusting block 61, the clamping mechanism 5 comprises a fixed block 51 fixedly connected to the adjusting block 61, and a placing block 44 for placing the gas flowmeter 22 is fixedly connected to the fixed block 51; the fixed block 51 is provided with a third chute 511, the axis of the third chute 511 is parallel to the axis of the first chute 421, the fixed blocks 51 on two sides of the placing block 44 are provided with connecting rods 52, and the connecting rods 52 slide in the third chute 511; clamping blocks 53 are fixedly connected to the two connecting rods 52, and the two clamping blocks 53 are abutted against the gas flowmeter 22.
The fixed block 51 is provided with a second adjusting component 54, the second adjusting component 54 comprises a bidirectional screw rod 542 rotatably connected in the third chute 511, the screw thread directions of two ends of the bidirectional screw rod 542 are opposite, the bidirectional screw rod 542 passes through the two connecting rods 52, and the two connecting rods 52 are respectively in threaded connection with two ends of the bidirectional screw rod 542; the fixed block 51 is fixedly connected with a second motor 541, and an output shaft of the second motor 541 is connected to one end of the bidirectional screw 542.
The support block 43 is provided with a positioning mechanism 7, the positioning mechanism 7 comprises a positioning plate 71 which is connected to the support block 43 in a sliding manner, and the positioning plate 71 is arranged perpendicular to the support block 43; a rack 72 is fixedly connected to the side wall of the positioning plate 71; the fourth motor 74 is fixedly connected to the supporting block 43, and a gear 73 meshed with the rack 72 is connected to an output shaft of the fourth motor 74 in a key manner.
The motors in this embodiment are all three-phase asynchronous motors.
The implementation principle of the bioreactor gas flow testing method in the embodiment of the application is as follows:
when detection is needed, the third control piece 16 seals the channel of the detection pipe 15, and then the first control piece 13 and the second control piece 14 are adjusted to enable gas to flow in the exhaust pipe 12; the gas does not enter the detection tube 15.
After the gas flows in the exhaust pipe 12 for a period of time, the third control member 16 is adjusted so that the gas can enter the detection pipe 15, and then the second control member 14 is adjusted so that the second control member 14 seals the passage of the exhaust pipe 12.
When the gas enters the detection tube 15, the fifth control member 25 seals the passage of the connection tube 21, and the gas flows from the detection tube 15; after a period of time, the fifth control 25 is adjusted so that gas can enter the connecting tube 21; the fourth control member 24 is adjusted so that the fourth control member 24 seals the passage of the detection tube 15, and after a certain period of time, the gas flow meter 22 on the connection tube 21 measures data.
When the data detected by the gas flow meters 22 close to the exhaust pipe 12 is smaller, the gas is detected by the gas flow meters 22 with a relatively large number, and the data measured by the plurality of gas flow meters 22 are averaged; when the data detected by the gas flow meter 22 near the exhaust pipe 12 is large, the gas will pass through a small amount of the gas flow meter 22 for detection, then the subsequent gas flow meter 22 will not detect the gas any more, and the average value of the data detected by the gas flow meter 22 is taken.
When the gas flowmeter 22 needs to be replaced, the gas flowmeter 22 is placed on the placement block 44, then the second motor 541 is started, the output shaft of the second motor 541 drives the bidirectional screw 542 to rotate, the bidirectional screw 542 drives the two connecting rods 52 to move in opposite directions, the clamping blocks 53 on the two connecting rods 52 move in opposite directions, and the two clamping blocks 53 clamp the gas flowmeter 22 located on the placement block 44.
Then, the fourth motor 74 is started, the output shaft of the fourth motor 74 drives the gear 73 to rotate, the gear 73 drives the rack 72 to move, and the rack 72 drives the positioning plate 71 to move.
Then the third motor 63 is started, the output shaft of the third motor 63 drives the second screw 62 to rotate, the second screw 62 drives the adjusting block 61 to move, the adjusting block 61 drives the fixing block 51 to move, the fixing block 51 drives the gas flowmeter 22 to move, and when the gas flowmeter 22 abuts against the positioning plate 71, the third motor 63 is turned off. The fourth motor 74 is then activated to extend the positioning plate 71 into the support block 43.
Finally, the first motor 451 is started, the output shaft of the first motor 451 drives the first screw 452 to rotate, the first screw 452 drives the supporting block 43 to move, the supporting block 43 drives the adjusting block 61 to move, and the adjusting block 61 moves to realize the switching of the two gas flow meters 22.
The foregoing are all preferred embodiments of the present application, and are not intended to limit the scope of the present application in any way, therefore: all equivalent changes in structure, shape and principle of this application should be covered in the protection scope of this application.

Claims (6)

1. A method for testing the gas flow rate of a bioreactor, comprising the steps of: s1: firstly, connecting an exhaust pipe (12) to a bioreactor (11);
s2: the exhaust pipe (12) is sequentially provided with a first control piece (13) and a second control piece (14) along the gas flow direction, a detection pipe (15) is arranged on the exhaust pipe (12) between the first control piece (13) and the second control piece (14), a detection unit (2) is arranged on the detection pipe (15), and a third control piece (16) is arranged on the detection pipe (15) between the detection unit (2) and the exhaust pipe (12);
s3: when detection is needed, the third control piece (16) is adjusted, so that the third control piece (16) seals a channel of the detection tube (15) and gas cannot enter the detection tube (15); adjusting the first control member (13) and the second control member (14) to enable the gas to flow in the exhaust pipe (12);
s4: after a period of time, the third control piece (16) is adjusted firstly, so that gas can enter the detection tube (15) through the exhaust tube (12), and then the second control piece (14) is adjusted, so that the second control piece (14) seals a channel of the exhaust tube (12); detecting the gas by a detection unit (2) on a detection tube (15) after a period of time to obtain a group of values; finally, the second control piece (14) is adjusted to enable the gas to flow in the exhaust pipe (12), and the third control piece (16) is adjusted to enable the third control piece (16) to seal a channel of the detection pipe (15);
s5: repeating S4, processing a plurality of groups of numerical values, and finally calculating a final numerical value;
the detection unit (2) comprises a connecting pipe (21), a gas flowmeter (22), a first one-way valve (23), a fourth control piece (24) and a fifth control piece (25), wherein the connecting pipe (21) is arranged on one side of the detection pipe (15), and two ends of the connecting pipe (21) are connected with the detection pipe (15);
the fourth control piece (24) is arranged on the detection tube (15) between the two ends of the connecting tube (21);
the fifth control member (25) is arranged at one end of the connecting pipe (21) close to the exhaust pipe (12), the first one-way valve (23) is arranged at the other end of the connecting pipe (21), and the gas flowmeter (22) is arranged on the connecting pipe (21) between the fifth control member (25) and the first one-way valve (23);
the detection units (2) are provided in plurality, and the measuring ranges of the gas flow meters (22) in the detection units (2) are gradually reduced along one end far away from the exhaust pipe (12).
2. A bioreactor gas flow testing method according to claim 1, characterized in that a gas outlet assembly (3) is arranged on the detection tube (15), the gas outlet assembly (3) comprises a first gas outlet tube (31), a second gas outlet tube (32), a third gas outlet tube (33), a second one-way valve (34) and a sixth control member (35), the first gas outlet tube (31) is provided with a plurality, and the first gas outlet tube (31) is arranged on the detection tube (15) between two adjacent detection units (2); the second air outlet pipe (32) is arranged at one side of the detection pipe (15), and a plurality of first air outlet pipes (31) are connected with the second air outlet pipe (32);
the third air outlet pipe (33) is arranged at one end, far away from the exhaust pipe (12), of the detection pipe (15), and the third air outlet pipe (33) is connected with the second air outlet pipe (32);
the first air outlet pipe (31) and the third air outlet pipe (33) are respectively provided with the second one-way valve (34) and the sixth control piece (35).
3. A bioreactor gas flow test method according to claim 1, characterized in that the detection unit (2) further comprises a support device (4), the support device (4) comprising a fixing frame (41), a support frame (42), a support block (43), a placement block (44) and a first adjusting assembly (45), the connecting tube (21) being arranged on the fixing frame (41);
the supporting block (43) is arranged on the supporting frame (42);
two placing blocks (44) are arranged, the two placing blocks (44) are arranged on the supporting block (43), and the gas flowmeter (22) is arranged on the placing blocks (44);
the first adjusting assembly (45) comprises a first motor (451) and a first screw rod (452), the first motor (451) is arranged on the supporting frame (42), the first screw rod (452) is connected with an output shaft of the first motor (451), and the first screw rod (452) penetrates through the supporting block (43) and is connected with the supporting block (43).
4. A bioreactor gas flow test method according to claim 3, characterized in that the support block (43) is provided with a clamping mechanism (5), the clamping mechanism (5) comprises a fixed block (51), a connecting rod (52), a clamping block (53) and a second adjusting assembly (54), the fixed block (51) is arranged on the support block (43), and the placing block (44) is arranged on the fixed block (51); the connecting rods (52) are slidably arranged on the fixed blocks (51) on two sides of the placing block (44), the clamping blocks (53) are arranged on the connecting rods (52), and the two clamping blocks (53) are abutted against the gas flowmeter (22);
the second adjusting assembly (54) comprises a second motor (541) and a bidirectional screw (542), wherein the second motor (541) is arranged on the fixed block (51); the bidirectional screw (542) is arranged on the output shaft of the second motor (541), the bidirectional screw (542) penetrates through the two connecting rods (52), and the two connecting rods (52) are respectively in threaded connection with two ends of the bidirectional screw (542).
5. A bioreactor gas flow test method according to claim 3, characterized in that the support block (43) is provided with an adjusting mechanism (6), the adjusting mechanism (6) comprises an adjusting block (61), a second screw (62) and a third motor (63), the adjusting block (61) is slidingly arranged on the support block (43), and the placing block (44) is arranged on the adjusting block (61);
the second screw (62) passes through the adjusting block (61) and is in threaded connection with the adjusting block (61); the third motor (63) is arranged on the supporting block (43), and an output shaft of the third motor (63) is connected with the second screw (62).
6. A bioreactor gas flow testing method according to claim 3, characterized in that a positioning mechanism (7) is arranged on the supporting block (43), the positioning mechanism (7) comprises a positioning plate (71), a rack (72), a gear (73) and a fourth motor (74), the positioning plate (71) is slidingly arranged on the supporting block (43), and the gas flowmeter (22) is abutted against the positioning plate (71); the rack (72) is arranged on the positioning plate (71);
the fourth motor (74) is arranged on the supporting block (43), and the gear (73) is arranged on an output shaft of the fourth motor (74) and meshed with the rack (72).
CN202111236225.3A 2021-10-22 2021-10-22 Bioreactor gas flow testing method Active CN113956966B (en)

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JPH0953965A (en) * 1995-08-15 1997-02-25 Asahi Tekuneion Kk Differential pressure flowmeter
CN104195033A (en) * 2014-09-09 2014-12-10 青岛汇君环境能源工程有限公司 Flat-plate type photobioreactor
CN206328205U (en) * 2016-12-22 2017-07-14 北京城市排水集团有限责任公司 Membrane bioreactor fluidised form test device
CN112730761A (en) * 2021-02-09 2021-04-30 河北名华质检技术服务有限公司 Detection equipment and detection method applied to air detection anti-pollution prediction
CN112823203A (en) * 2018-10-17 2021-05-18 环球生命科技咨询美国有限责任公司 Bioreactor system
CN213986357U (en) * 2020-09-11 2021-08-17 深圳市泰诚检测有限公司 Industrial waste gas detection device

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ITMI20132045A1 (en) * 2013-12-09 2015-06-10 Milano Politecnico METHOD AND EQUIPMENT FOR MEASURING THE FLOW OF A GASEOUS FLOW
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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0953965A (en) * 1995-08-15 1997-02-25 Asahi Tekuneion Kk Differential pressure flowmeter
CN104195033A (en) * 2014-09-09 2014-12-10 青岛汇君环境能源工程有限公司 Flat-plate type photobioreactor
CN206328205U (en) * 2016-12-22 2017-07-14 北京城市排水集团有限责任公司 Membrane bioreactor fluidised form test device
CN112823203A (en) * 2018-10-17 2021-05-18 环球生命科技咨询美国有限责任公司 Bioreactor system
CN213986357U (en) * 2020-09-11 2021-08-17 深圳市泰诚检测有限公司 Industrial waste gas detection device
CN112730761A (en) * 2021-02-09 2021-04-30 河北名华质检技术服务有限公司 Detection equipment and detection method applied to air detection anti-pollution prediction

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