CN113956966A - Bioreactor gas flow testing method - Google Patents

Bioreactor gas flow testing method Download PDF

Info

Publication number
CN113956966A
CN113956966A CN202111236225.3A CN202111236225A CN113956966A CN 113956966 A CN113956966 A CN 113956966A CN 202111236225 A CN202111236225 A CN 202111236225A CN 113956966 A CN113956966 A CN 113956966A
Authority
CN
China
Prior art keywords
pipe
gas
detection
block
adjusting
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN202111236225.3A
Other languages
Chinese (zh)
Other versions
CN113956966B (en
Inventor
陈超
卜兴
李达源
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shanghai Zhihe Biotechnology Co ltd
Original Assignee
Shanghai Zhihe Biotechnology Co ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shanghai Zhihe Biotechnology Co ltd filed Critical Shanghai Zhihe Biotechnology Co ltd
Priority to CN202111236225.3A priority Critical patent/CN113956966B/en
Publication of CN113956966A publication Critical patent/CN113956966A/en
Application granted granted Critical
Publication of CN113956966B publication Critical patent/CN113956966B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/48Holding appliances; Racks; Supports
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/50Means for positioning or orientating the apparatus
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/20Degassing; Venting; Bubble traps
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • C12M37/04Seals
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Clinical Laboratory Science (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

The application relates to a bioreactor gas flow testing method, which comprises the following steps of S1: connecting an exhaust pipe to the bioreactor; s2: the exhaust pipe is provided with a first control piece, a second control piece and a detection pipe, and the detection pipe is provided with a detection unit and a third control piece; s3: when the gas detection device needs to detect, the first control part, the second control part and the third control part are adjusted to enable gas to flow in the exhaust pipe and not to enter the detection pipe; s4: after a period of time, adjusting the third control element and the second control element to enable gas to enter the detection pipe through the exhaust pipe and not to circulate in the exhaust pipe; after a period of time, the detection unit on the detection tube detects the gas to obtain a group of values; finally, the second control piece and the third control piece are adjusted, so that the gas flows in the exhaust pipe and cannot enter the detection pipe; s5: s3 is repeated to process the plurality of sets of values, and the final value is finally calculated. The application has the effect of detecting the accuracy.

Description

Bioreactor gas flow testing method
Technical Field
The application relates to the technical field of gas detection, in particular to a gas flow testing method for a bioreactor.
Background
The bioreactor refers to a reaction system in which a liquid phase or a solid phase is inoculated with naturally occurring microorganisms or microorganisms having a specific degradation ability. After the bioreactor generates gas, the gas generated by the bioreactor needs to be detected.
At present, the conventional detection is that an exhaust pipe is directly connected to a bioreactor, a control valve is installed at one end of the exhaust pipe close to the bioreactor, and a gas flowmeter is installed on the exhaust pipe. And opening the control valve, enabling the gas generated by the bioreactor to enter the exhaust pipe, and detecting the flow of the gas entering the exhaust pipe by using the gas flowmeter.
In the process of implementing the application, the inventor finds that at least the following problems exist in the technology: the gas that bioreactor produced can enter into the blast pipe when opening the control in, initial gaseous ratio is many and also can produce certain impact force, and when the impact force was used gas flowmeter, can lead to certain damage to gas flowmeter, gas flowmeter damages the back, and the manual work is difficult to be observed, thereby leads to detecting inaccurate when continuing to detect gas flow with the gas flowmeter of damage.
Disclosure of Invention
In order to improve the detection accuracy, the application provides a bioreactor gas flow testing method.
The gas flow testing method for the bioreactor adopts the following technical scheme:
a bioreactor gas flow testing method comprises the following steps: s1: firstly, connecting an exhaust pipe to a bioreactor; s2: the gas detection device comprises an exhaust pipe, a first control piece and a second control piece, wherein the exhaust pipe is sequentially provided with the first control piece and the second control piece along the gas flowing direction; s3: when the gas detection device needs to detect, adjusting the third control part to seal the channel of the detection pipe so that the gas cannot enter the detection pipe; adjusting the first control member and the second control member to enable gas to flow in the exhaust pipe; s4: after a period of time, firstly adjusting the third control part to enable gas to enter the detection pipe through the exhaust pipe, and then adjusting the second control part to enable the second control part to seal a channel of the exhaust pipe; after a period of time, the detection unit on the detection tube detects the gas to obtain a group of values; finally, the second control piece is adjusted to enable the gas to flow in the exhaust pipe again, and the third control piece is adjusted to enable the third control piece to seal the channel of the detection pipe; s5: s4 is repeated to process the plurality of sets of values, and the final value is finally calculated.
By adopting the technical scheme, when the gas flow generated in the bioreactor needs to be detected, the third control part is firstly used for sealing the channel of the detection pipe, and then the first control part and the second control part are adjusted to enable the gas to flow in the exhaust pipe; after the gas flows in the exhaust pipe for a period of time, the third control part is adjusted to enable the gas to flow in the detection pipe again, then the second control part is adjusted to enable the second control part to seal a channel of the exhaust pipe, and the detection unit on the detection pipe can detect the gas which flows stably in the detection pipe to obtain a group of numerical values; then, repeatedly detecting by using the gas flowmeter, and finally detecting a plurality of groups of data; the set testing method can improve the detection accuracy.
Optionally, the detection unit includes a connection pipe, a gas flowmeter, a first check valve, a fourth control element and a fifth control element, the connection pipe is disposed at one side of the detection pipe, and both ends of the connection pipe are connected to the detection pipe; the fourth control piece is arranged on the detection pipe between two ends of the connecting pipe; the fifth control piece is arranged at one end, close to the exhaust pipe, of the connecting pipe, the first one-way valve is arranged at the other end of the connecting pipe, and the gas flow meter is arranged on the connecting pipe between the fifth control piece and the first one-way valve.
By adopting the technical scheme, when gas enters the detection pipe, the fifth control piece seals the channel of the connecting pipe, and the gas enters the detection pipe; after a period of time, adjusting the fifth control member to enable gas to enter the connecting pipe; and adjusting the fourth control piece to enable the fourth control piece to seal the channel of the detection pipe, and after a period of time, enabling the data measured by the gas flowmeter on the connecting pipe to be the required data.
Optionally, the number of the detection units is multiple, and the measuring range of the gas flowmeter in the multiple detection units is gradually reduced along one end far away from the exhaust pipe.
By adopting the technical scheme, when the data detected by the gas flow meter close to the exhaust pipe is small, the gas is detected by a plurality of gas flow meters, and the average value of the data detected by the plurality of gas flow meters is taken; when the data that the gas flowmeter that is close to the blast pipe detected is great, gas can detect through a small amount of gas flowmeter, and then subsequent gas flowmeter can not detect gas again, gets the average value of gas flowmeter survey data.
Optionally, the gas outlet assembly is arranged on the detection tube, and the gas outlet assembly includes a plurality of first gas outlet tubes, a plurality of second gas outlet tubes, a plurality of third gas outlet tubes, a plurality of second check valves and a plurality of sixth control elements, and the first gas outlet tubes are arranged on the detection tube between two adjacent detection units; the second air outlet pipe is arranged on one side of the detection pipe, and the plurality of first air outlet pipes are connected with the second air outlet pipe; the third air outlet pipe is arranged at one end of the detection pipe far away from the exhaust pipe and is connected with the second air outlet pipe; the first air outlet pipe and the third air outlet pipe are both provided with the second one-way valve and the sixth control piece.
By adopting the technical scheme, when gas is detected by the gas flow meters of a certain number of detection units, the sixth control piece on the first gas outlet pipe between the last used detection unit and the first unused detection unit is opened, and the other sixth control pieces seal the channels of the other first gas outlet pipes, so that the gas can enter the second gas outlet pipe through the first gas outlet pipe and is finally discharged; when the gas is processed by the gas flow meters in all the detection units, the channels of all the first connecting pipes are sealed by the sixth control element, and then the gas enters the second gas outlet pipe through the third gas outlet pipe and is finally discharged.
Optionally, the detection unit further includes a supporting device, the supporting device includes a fixing frame, a supporting block, a placing block and a first adjusting assembly, and the connecting pipe is disposed on the fixing frame; the supporting block is arranged on the supporting frame; the number of the placing blocks is two, the two placing blocks are arranged on the supporting block, and the gas flowmeter is arranged on the placing blocks; the first adjusting assembly comprises a first motor and a first screw rod, the first motor is arranged on the supporting frame, the first screw rod is connected with an output shaft of the first motor, and the first screw rod penetrates through the supporting block and is connected with the supporting block.
By adopting the technical scheme, the detection tube is supported by the arranged fixing frame; the placing block on the supporting block supports the gas flowmeter. Need change when the gas flowmeter damage, perhaps need change when the gas flowmeter adjusts measuring range, place new gas flowmeter on one places the piece earlier, then make the output shaft of first motor drive first screw rod and rotate, first screw rod drives the supporting shoe and moves on the support frame, makes the gas flowmeter that new gas flowmeter replacement needs to be changed.
Optionally, a clamping mechanism is arranged on the supporting block, the clamping mechanism comprises a fixed block, a connecting rod, a clamping block and a second adjusting assembly, the fixed block is arranged on the supporting block, and the placing block is arranged on the fixed block; the connecting rods are arranged on the fixed blocks on the two sides of the placing block in a sliding mode, the clamping blocks are arranged on the connecting rods, and the two clamping blocks are abutted against the gas flowmeter; the second adjusting assembly comprises a second motor and a bidirectional screw rod, and the second motor is arranged on the fixed block; the bidirectional screw rod is arranged on an output shaft of the second motor, the bidirectional screw rod penetrates through the two connecting rods, and the two connecting rods are respectively in threaded connection with two ends of the bidirectional screw rod.
By adopting the technical scheme, after the gas flowmeter is arranged on the placing block, the output shaft of the second motor drives the two-way screw to rotate, the two-way screw drives the two connecting rods to move in opposite directions, and the clamping blocks on the two connecting rods are abutted against the gas flowmeter; the fixture who sets up can improve the stability of gas flowmeter on placing the piece.
Optionally, an adjusting mechanism is arranged on the supporting block, the adjusting mechanism includes an adjusting block, a second screw and a third motor, the adjusting block is slidably arranged on the supporting block, and the placing block is arranged on the adjusting block; the second screw rod penetrates through the adjusting block and is in threaded connection with the adjusting block; the third motor is arranged on the supporting block, and an output shaft of the third motor is connected with the second screw rod.
Through adopting above-mentioned technical scheme, the third motor drives the second screw rod and rotates, and the second screw rod rotates and will drive the adjusting block motion, and the adjusting block drives the gas flowmeter motion to reduce artifical adjustment, improve work efficiency.
Optionally, a positioning mechanism is arranged on the supporting block, the positioning mechanism includes a positioning plate, a rack, a gear and a fourth motor, the positioning plate is slidably arranged on the supporting block, and the gas flowmeter abuts against the positioning plate; the rack is arranged on the positioning plate; the fourth motor is arranged on the supporting block, and the gear is arranged on an output shaft of the fourth motor and meshed with the rack.
By adopting the technical scheme, the output shaft of the fourth motor drives the gear to rotate, the rack meshed with the gear drives the positioning plate to move, and a part of the positioning plate extends out of the supporting block; when the gas flowmeter is abutted against the positioning plate, the gas flowmeter is positioned at the optimal position, so that the gas flowmeter is convenient to replace; the positioning mechanism who sets up can realize fixing a position gas flowmeter to reduce the time raise the efficiency of artifical adjustment.
To sum up, the application comprises the following beneficial technical effects:
1. the set testing method can improve the detection accuracy;
2. the positioning mechanism who sets up can realize fixing a position gas flowmeter to reduce the time raise the efficiency of artifical adjustment.
Drawings
FIG. 1 is a schematic structural view of a detection tube connected to an exhaust pipe in an embodiment of the present application;
FIG. 2 is a schematic structural diagram of a first adjustment assembly in an embodiment of the present application;
FIG. 3 is a schematic structural diagram of an adjusting mechanism according to an embodiment of the present application;
fig. 4 is a schematic structural diagram of a positioning mechanism in an embodiment of the present application.
Reference numerals: 11. a bioreactor; 12. an exhaust pipe; 13. a first control member; 14. a second control member; 15. a detection tube; 16. a third control; 17. a mounting frame; 18. a connecting frame; 2. a detection unit; 21. a connecting pipe; 22. a gas flow meter; 23. a first check valve; 24. a fourth control member; 25. a fifth control member; 3. an air outlet assembly; 31. a first air outlet pipe; 32. a second air outlet pipe; 33. a third air outlet pipe; 34. a second one-way valve; 35. a sixth control member; 4. a support device; 41. a fixed mount; 42. a support frame; 421. a first chute; 43. a support block; 431. a second chute; 44. placing the blocks; 45. a first adjustment assembly; 451. a first motor; 452. a first screw; 5. a clamping mechanism; 51. a fixed block; 511. a third chute; 52. a connecting rod; 53. a clamping block; 54. a second adjustment assembly; 541. a second motor; 542. a bidirectional screw; 6. an adjustment mechanism; 61. an adjusting block; 62. a second screw; 63. a third motor; 7. a positioning mechanism; 71. positioning a plate; 72. a rack; 73. a gear; 74. and a fourth motor.
Detailed Description
The present application is described in further detail below with reference to figures 1-4. In the embodiment, the first control element 13, the second control element 14, the third control element 16, the fourth control element 24, the fifth control element 25 and the sixth control element 35 are all control valves.
The embodiment of the application discloses a bioreactor gas flow testing method.
Referring to fig. 1 and 2, the bioreactor gas flow rate test method includes the steps of:
s1: the exhaust pipe 12 is first connected to the bioreactor 11.
S2: the exhaust pipe 12 is provided with a first control part 13 and a second control part 14 along the gas flowing direction, a detection pipe 15 is installed on the exhaust pipe 12 between the first control part 13 and the second control part 14, a plurality of groups of detection units 2 are arranged on the detection pipe 15, and a third control part 16 is installed on the detection pipe 15 between the detection unit 2 closest to the exhaust pipe 12 and the exhaust pipe 12.
The detection unit 2 comprises a connecting pipe 21 arranged on one side of the detection pipe 15, two ends of the connecting pipe 21 are connected to the detection pipe 15, one end, close to the exhaust pipe 12, of the connecting pipe 21 is connected with a fifth control element 25, and one end, far away from the fifth control element 25, of the connecting pipe 21 is connected with a first one-way valve 23; a gas flow meter 22 is connected to the connection pipe 21 between the fifth connection member and the first check valve 23. The fourth control member 24 is connected to the sensing tube 15 between both ends of the connection tube 21. And the range of the gas flow meter 22 in the detection unit 2 becomes gradually smaller toward the end away from the exhaust pipe 12.
S3: when the detection is needed, the third control part 16 is firstly sealed on the passage of the detection pipe 15, and then the first control part 13 and the second control part 14 are adjusted to enable the gas to flow in the exhaust pipe 12; gas does not enter the sensing tube 15.
S4: after the gas flows in the exhaust pipe 12 for a period of time, the third control element 16 is adjusted to allow the gas to enter the detection pipe 15, and then the second control element 14 is adjusted to seal the passage of the exhaust pipe 12 by the second control element 14.
When the gas enters into the detecting tube 15, the fifth control member 25 seals the passage of the connection tube 21, and the gas flows in the detecting tube 15; after a period of time, the fifth control element 25 is adjusted to allow the gas to enter the connection tube 21; the fourth control member 24 is adjusted so that the fourth control member 24 seals the passage of the sensing tube 15, and after a certain time interval, the gas flowmeter 22 on the connecting tube 21 measures the data.
When the data detected by the gas flowmeter 22 close to the exhaust pipe 12 is small, the gas is detected by a plurality of gas flowmeters 22, and the data detected by the plurality of gas flowmeters 22 are averaged; when the data detected by the gas flow meter 22 near the exhaust pipe 12 is large, the gas will be detected by a small number of gas flow meters 22, and then the subsequent gas flow meters 22 will not detect the gas any more, and the data detected by the gas flow meters 22 is averaged.
S5: s4 is repeated to process the plurality of sets of values, and the final value is finally calculated.
Referring to fig. 1 and 2, the gas outlet assembly 3 is arranged on the detection tube 15, one end of the detection tube 15, which is far away from the exhaust tube 12, is connected with a third gas outlet tube 33, and the third gas outlet tube 33 is sequentially connected with a sixth control element 35 and a second one-way valve 34 along the direction far away from the detection tube 15; the connecting frame 18 is placed on one side, far away from the connecting pipe 21, of the detecting pipe 15, the second air outlet pipe 32 is placed on the connecting frame 18, and one end, far away from the detecting pipe 15, of the third air outlet pipe 33 is connected with the second air outlet pipe 32.
The first air outlet pipe 31 is connected to the detection pipe 15 between two adjacent detection units 2, the first air outlet pipe 31 is sequentially connected with the sixth control element 35 and the second one-way valve 34 along the direction far away from the detection pipe 15, and one end, far away from the detection pipe 15, of the first air outlet pipe 31 is connected with the second air outlet pipe 32.
When the gas is performed by the gas flow meters 22 in all the sensing units 2, all the passages of the first connection pipe 21 are sealed by the sixth control member 35, and then the gas is introduced into the second gas outlet pipe 32 through the third gas outlet pipe 33 and finally discharged.
When gas is detected by the gas flow meters 22 of a certain number of detecting units 2, the sixth control element 35 on the first gas outlet pipe 31 between the last used detecting unit 2 and the first unused detecting unit 2 is opened, and the other sixth control elements 35 seal the channels of the other first gas outlet pipes 31, so that the gas can enter the second gas outlet pipe 32 through the first gas outlet pipe 31 and finally be discharged.
Referring to fig. 1 and 3, the exhaust pipe 12 is provided at one side thereof with a mounting bracket 17, and the mounting bracket 17 supports the sensing tube 15.
The exhaust pipe 12 is provided with a support device 4 on one side thereof, and the support device 4 includes a holder 41 for supporting the connection pipe 21.
A supporting frame 42 is placed on one side, away from the detection tube 15, of the fixing frame 41, a first sliding groove 421 is formed in the supporting frame 42, a supporting block 43 is connected in the first sliding groove 421 in a sliding manner, a first adjusting assembly 45 is arranged on the supporting frame 42, the first adjusting assembly 45 comprises a first screw 452 rotatably connected in the first sliding groove 421, and the first screw 452 penetrates through the supporting block 43 and is in threaded connection with the supporting block 43; the supporting frame 42 is fixedly connected with a first motor 451, and an output shaft of the first motor 451 is connected with one end of a first screw 452.
Referring to fig. 2 and 3, two adjusting mechanisms 6 are disposed on the supporting block 43, a second sliding groove 431 is disposed on a side of the supporting block 43 away from the supporting frame 42, and an axis of the second sliding groove 431 is perpendicular to an axis of the first sliding groove 421.
The adjusting mechanism 6 comprises an adjusting block 61 connected in the second sliding groove 431 in a sliding manner; a second screw 62 is rotatably connected in the second sliding groove 431, and a second bolt passes through the adjusting block 61 and is in threaded connection with the adjusting block 61; a third motor 63 is fixedly connected to the supporting block 43, and an output shaft of the third motor 63 is connected to one end of the second screw 62.
The adjusting block 61 is provided with a clamping mechanism 5, the clamping mechanism 5 comprises a fixed block 51 fixedly connected to the adjusting block 61, and the fixed block 51 is fixedly connected with a placing block 44 for placing the gas flowmeter 22; the fixed block 51 is provided with a third sliding chute 511, the axis of the third sliding chute 511 is parallel to the axis of the first sliding chute 421, the fixed blocks 51 on the two sides of the placing block 44 are both provided with a connecting rod 52, and the connecting rod 52 slides in the third sliding chute 511; the two connecting rods 52 are fixedly connected with clamping blocks 53, and the two clamping blocks 53 are tightly propped against the gas flowmeter 22.
The fixed block 51 is provided with a second adjusting assembly 54, the second adjusting assembly 54 comprises a bidirectional screw 542 which is rotatably connected in the third sliding groove 511, the thread directions of two ends of the bidirectional screw 542 are opposite, the bidirectional screw 542 penetrates through the two connecting rods 52, and the two connecting rods 52 are respectively in thread connection with two ends of the bidirectional screw 542; the fixed block 51 is fixedly connected with a second motor 541, and an output shaft of the second motor 541 is connected with one end of the bidirectional screw 542.
The supporting block 43 is provided with a positioning mechanism 7, the positioning mechanism 7 comprises a positioning plate 71 connected to the supporting block 43 in a sliding manner, and the positioning plate 71 is perpendicular to the supporting block 43; a rack 72 is fixedly connected to the side wall of the positioning plate 71; a fourth motor 74 is fixedly connected to the supporting block 43, and a gear 73 engaged with the rack 72 is keyed on an output shaft of the fourth motor 74.
The motors in this embodiment are all three-phase asynchronous motors.
The implementation principle of the bioreactor gas flow testing method in the embodiment of the application is as follows:
when detection is needed, the third control part 16 is firstly sealed on the channel of the detection pipe 15, and then the first control part 13 and the second control part 14 are adjusted to enable gas to flow in the exhaust pipe 12; gas does not enter the sensing tube 15.
After the gas flows in the exhaust pipe 12 for a period of time, the third control element 16 is adjusted to allow the gas to enter the detection pipe 15, and then the second control element 14 is adjusted to seal the passage of the exhaust pipe 12 by the second control element 14.
When the gas enters into the detecting tube 15, the fifth control member 25 seals the passage of the connection tube 21, and the gas flows from the detecting tube 15; after a period of time, the fifth control element 25 is adjusted to allow the gas to enter the connection tube 21; the fourth control member 24 is adjusted so that the fourth control member 24 seals the passage of the sensing tube 15, and after a certain time interval, the gas flowmeter 22 on the connecting tube 21 measures the data.
When the data detected by the gas flowmeter 22 close to the exhaust pipe 12 is small, the gas is detected by a plurality of gas flowmeters 22, and the data detected by the plurality of gas flowmeters 22 are averaged; when the data detected by the gas flow meter 22 near the exhaust pipe 12 is large, the gas will be detected by a small number of gas flow meters 22, and then the subsequent gas flow meters 22 will not detect the gas any more, and the data detected by the gas flow meters 22 is averaged.
When the gas flowmeter 22 needs to be replaced, the gas flowmeter 22 is placed on the placing block 44, then the second motor 541 is started, the output shaft of the second motor 541 drives the two-way screw 542 to rotate, the two-way screw 542 drives the two connecting rods 52 to move in opposite directions, the clamping blocks 53 on the two connecting rods 52 move in opposite directions, and the two clamping blocks 53 clamp the gas flowmeter 22 on the placing block 44.
Then, the fourth motor 74 is started, the output shaft of the fourth motor 74 drives the gear 73 to rotate, the gear 73 drives the rack 72 to move, and the rack 72 drives the positioning plate 71 to move.
Then, the third motor 63 is started, the output shaft of the third motor 63 drives the second screw 62 to rotate, the second screw 62 drives the adjusting block 61 to move, the adjusting block 61 drives the fixing block 51 to move, the fixing block 51 drives the gas flowmeter 22 to move, and when the gas flowmeter 22 abuts against the positioning plate 71, the third motor 63 is turned off. The fourth motor 74 is then activated to extend the positioning plate 71 into the support block 43.
Finally, the first motor 451 is started, an output shaft of the first motor 451 drives the first screw 452 to rotate, the first screw 452 drives the supporting block 43 to move, the supporting block 43 drives the adjusting block 61 to move, and the two gas flow meters 22 can be switched by the movement of the adjusting block 61.
The above embodiments are preferred embodiments of the present application, and the protection scope of the present application is not limited by the above embodiments, so: all equivalent changes made according to the structure, shape and principle of the present application shall be covered by the protection scope of the present application.

Claims (8)

1. A bioreactor gas flow testing method is characterized by comprising the following steps: s1: firstly, connecting an exhaust pipe (12) to a bioreactor (11);
s2: a first control piece (13) and a second control piece (14) are sequentially arranged on the exhaust pipe (12) along the gas flowing direction, a detection pipe (15) is installed on the exhaust pipe (12) between the first control piece (13) and the second control piece (14), a detection unit (2) is arranged on the detection pipe (15), and a third control piece (16) is arranged on the detection pipe (15) between the detection unit (2) and the exhaust pipe (12);
s3: when the detection is needed, adjusting the third control part (16) to enable the third control part (16) to seal the passage of the detection pipe (15) so that gas cannot enter the detection pipe (15); adjusting the first control element (13) and the second control element (14) to enable a gas to flow in the exhaust pipe (12);
s4: after a period of time, firstly adjusting the third control part (16) to enable gas to enter the detection pipe (15) through the exhaust pipe (12), and then adjusting the second control part (14) to enable the second control part (14) to seal the passage of the exhaust pipe (12); after a period of time, the detection unit (2) on the detection tube (15) detects the gas to obtain a group of values; finally, the second control element (14) is adjusted to enable gas to flow in the exhaust pipe (12), and the third control element (16) is adjusted to enable the third control element (16) to seal the passage of the detection pipe (15);
s5: s4 is repeated to process the plurality of sets of values, and the final value is finally calculated.
2. A bioreactor gas flow rate test method according to claim 1, characterized in that the detection unit (2) comprises a connection pipe (21), a gas flow meter (22), a first one-way valve (23), a fourth control element (24) and a fifth control element (25), the connection pipe (21) is arranged at one side of the detection pipe (15), and both ends of the connection pipe (21) are connected with the detection pipe (15);
the fourth control member (24) is arranged on the detection pipe (15) between two ends of the connecting pipe (21);
the fifth control part (25) is arranged at one end of the connecting pipe (21) close to the exhaust pipe (12), the first one-way valve (23) is arranged at the other end of the connecting pipe (21), and the gas flow meter (22) is arranged on the connecting pipe (21) between the fifth control part (25) and the first one-way valve (23).
3. The bioreactor gas flow rate test method according to claim 2, wherein a plurality of detection units (2) are provided, and the range of the gas flowmeter (22) in the plurality of detection units (2) is gradually reduced along the end far away from the exhaust pipe (12).
4. The bioreactor gas flow testing method according to claim 3, characterized in that a gas outlet assembly (3) is arranged on the detection tube (15), the gas outlet assembly (3) comprises a plurality of first gas outlet tubes (31), a plurality of second gas outlet tubes (32), a plurality of third gas outlet tubes (33), a plurality of second one-way valves (34) and a sixth control element (35), and the first gas outlet tubes (31) are arranged on the detection tube (15) between two adjacent detection units (2); the second air outlet pipe (32) is arranged on one side of the detection pipe (15), and the plurality of first air outlet pipes (31) are connected with the second air outlet pipe (32);
the third air outlet pipe (33) is arranged at one end of the detection pipe (15) far away from the exhaust pipe (12), and the third air outlet pipe (33) is connected with the second air outlet pipe (32);
the first air outlet pipe (31) and the third air outlet pipe (33) are both provided with the second one-way valve (34) and the sixth control piece (35).
5. The bioreactor gas flow test method according to claim 2, wherein the detection unit (2) further comprises a support device (4), the support device (4) comprises a fixing frame (41), a support frame (42), a support block (43), a placing block (44) and a first adjusting component (45), the connecting pipe (21) is arranged on the fixing frame (41);
the supporting block (43) is arranged on the supporting frame (42);
two placing blocks (44) are arranged, the two placing blocks (44) are arranged on the supporting block (43), and the gas flowmeter (22) is arranged on the placing blocks (44);
the first adjusting assembly (45) comprises a first motor (451) and a first screw rod (452), the first motor (451) is arranged on the supporting frame (42), the first screw rod (452) is connected with an output shaft of the first motor (451), and the first screw rod (452) penetrates through the supporting block (43) and is connected with the supporting block (43).
6. The bioreactor gas flow testing method according to claim 5, characterized in that a clamping mechanism (5) is arranged on the supporting block (43), the clamping mechanism (5) comprises a fixed block (51), a connecting rod (52), a clamping block (53) and a second adjusting component (54), the fixed block (51) is arranged on the supporting block (43), and the placing block (44) is arranged on the fixed block (51); the connecting rods (52) are arranged on the fixed blocks (51) on the two sides of the placing block (44) in a sliding mode, the clamping blocks (53) are arranged on the connecting rods (52), and the two clamping blocks (53) are abutted against the gas flowmeter (22);
the second adjusting assembly (54) comprises a second motor (541) and a bidirectional screw (542), and the second motor (541) is arranged on the fixed block (51); the bidirectional screw (542) is arranged on an output shaft of the second motor (541), the bidirectional screw (542) penetrates through the two connecting rods (52), and the two connecting rods (52) are respectively in threaded connection with two ends of the bidirectional screw (542).
7. The bioreactor gas flow test method according to claim 5, wherein an adjusting mechanism (6) is arranged on the supporting block (43), the adjusting mechanism (6) comprises an adjusting block (61), a second screw (62) and a third motor (63), the adjusting block (61) is slidably arranged on the supporting block (43), and the placing block (44) is arranged on the adjusting block (61);
the second screw (62) penetrates through the adjusting block (61) and is in threaded connection with the adjusting block (61); the third motor (63) is arranged on the supporting block (43), and an output shaft of the third motor (63) is connected with the second screw (62).
8. The bioreactor gas flow testing method according to claim 5, characterized in that a positioning mechanism (7) is arranged on the supporting block (43), the positioning mechanism (7) comprises a positioning plate (71), a rack (72), a gear (73) and a fourth motor (74), the positioning plate (71) is slidably arranged on the supporting block (43), and the gas flowmeter (22) abuts against the positioning plate (71); the rack (72) is arranged on the positioning plate (71);
the fourth motor (74) is arranged on the supporting block (43), and the gear (73) is arranged on an output shaft of the fourth motor (74) and meshed with the rack (72).
CN202111236225.3A 2021-10-22 2021-10-22 Bioreactor gas flow testing method Active CN113956966B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202111236225.3A CN113956966B (en) 2021-10-22 2021-10-22 Bioreactor gas flow testing method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202111236225.3A CN113956966B (en) 2021-10-22 2021-10-22 Bioreactor gas flow testing method

Publications (2)

Publication Number Publication Date
CN113956966A true CN113956966A (en) 2022-01-21
CN113956966B CN113956966B (en) 2023-08-01

Family

ID=79466216

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202111236225.3A Active CN113956966B (en) 2021-10-22 2021-10-22 Bioreactor gas flow testing method

Country Status (1)

Country Link
CN (1) CN113956966B (en)

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0953965A (en) * 1995-08-15 1997-02-25 Asahi Tekuneion Kk Differential pressure flowmeter
CN104195033A (en) * 2014-09-09 2014-12-10 青岛汇君环境能源工程有限公司 Flat-plate type photobioreactor
US20160032233A1 (en) * 2014-08-02 2016-02-04 Cashido Corporation Aeration apparatus, aeration method and cleaning method
US20160377469A1 (en) * 2013-12-09 2016-12-29 Politecnico Di Milano Method and Apparatus for Measuring Gas Flow
CN206328205U (en) * 2016-12-22 2017-07-14 北京城市排水集团有限责任公司 Membrane bioreactor fluidised form test device
CN112730761A (en) * 2021-02-09 2021-04-30 河北名华质检技术服务有限公司 Detection equipment and detection method applied to air detection anti-pollution prediction
CN112823203A (en) * 2018-10-17 2021-05-18 环球生命科技咨询美国有限责任公司 Bioreactor system
CN213986357U (en) * 2020-09-11 2021-08-17 深圳市泰诚检测有限公司 Industrial waste gas detection device

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0953965A (en) * 1995-08-15 1997-02-25 Asahi Tekuneion Kk Differential pressure flowmeter
US20160377469A1 (en) * 2013-12-09 2016-12-29 Politecnico Di Milano Method and Apparatus for Measuring Gas Flow
US20160032233A1 (en) * 2014-08-02 2016-02-04 Cashido Corporation Aeration apparatus, aeration method and cleaning method
CN104195033A (en) * 2014-09-09 2014-12-10 青岛汇君环境能源工程有限公司 Flat-plate type photobioreactor
CN206328205U (en) * 2016-12-22 2017-07-14 北京城市排水集团有限责任公司 Membrane bioreactor fluidised form test device
CN112823203A (en) * 2018-10-17 2021-05-18 环球生命科技咨询美国有限责任公司 Bioreactor system
CN213986357U (en) * 2020-09-11 2021-08-17 深圳市泰诚检测有限公司 Industrial waste gas detection device
CN112730761A (en) * 2021-02-09 2021-04-30 河北名华质检技术服务有限公司 Detection equipment and detection method applied to air detection anti-pollution prediction

Also Published As

Publication number Publication date
CN113956966B (en) 2023-08-01

Similar Documents

Publication Publication Date Title
CN105865589B (en) Intelligent full flow point full-automatic water meter calibration equipment
CN107014598B (en) A kind of air valve air inlet performance testing device and test method
CN110873286B (en) Multiple air source supply device for high-pressure large-flow gas experiment
CN113956966B (en) Bioreactor gas flow testing method
CN110849582A (en) Multiphase flow experimental device
CN100338454C (en) Rotary gas collector
CN219714639U (en) Water meter tightness detection device
CN212513604U (en) Multifunctional double-valve fatigue testing machine
CN220207063U (en) Flow valve accuracy detection table
CN113252529B (en) High-temperature gas-driven penetration testing system and method for metal pipe fitting
CN215065703U (en) Pipeline flow erosion corrosion test device simulates
CN105784341A (en) Standard device for checking flow and differential pressure of constant flow valve and checking method
CN209230744U (en) A kind of wide-range verifies standard set-up than water meter
CN208833501U (en) A kind of zero-pressure valve test device
RU2239795C2 (en) Device for testing gas meters
CN215984772U (en) Flow calibrating device workbench with replaceable test caliber
CN212340376U (en) Manual meter clamping device and manual water flow calibrating device with same
CN219015426U (en) Calibrating device of flowmeter
CN109405937B (en) Wide-range ratio water meter calibration standard device and water meter calibration method thereof
CN220418542U (en) Electromagnetic flowmeter convenient to installation
CN218121103U (en) Calibration device for flow testing tool of electromagnetic flowmeter
CN220337189U (en) Servo hydraulic valve endurance test device
CN219798506U (en) Flowmeter detection device with switchable detection interfaces
CN215573268U (en) Ultrasonic water meter calibration table based on instantaneous flow method
CN220708504U (en) Elliptic gear flowmeter calibrating device

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant