CN113951937A - Oral mucus collection system for PCR detection of hog cholera virus - Google Patents
Oral mucus collection system for PCR detection of hog cholera virus Download PDFInfo
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- CN113951937A CN113951937A CN202111111297.5A CN202111111297A CN113951937A CN 113951937 A CN113951937 A CN 113951937A CN 202111111297 A CN202111111297 A CN 202111111297A CN 113951937 A CN113951937 A CN 113951937A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B10/00—Other methods or instruments for diagnosis, e.g. instruments for taking a cell sample, for biopsy, for vaccination diagnosis; Sex determination; Ovulation-period determination; Throat striking implements
- A61B10/0045—Devices for taking samples of body liquids
- A61B10/0051—Devices for taking samples of body liquids for taking saliva or sputum samples
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D15/00—Mouth openers
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D3/00—Appliances for supporting or fettering animals for operative purposes
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B2503/00—Evaluating a particular growth phase or type of persons or animals
- A61B2503/40—Animals
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D3/00—Appliances for supporting or fettering animals for operative purposes
- A61D2003/003—Appliances for supporting or fettering animals for operative purposes with head or neck restraining means
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The invention belongs to the technical field of animal experimental articles, and particularly relates to an oral mucus collecting device for PCR detection of hog cholera virus, which comprises a cage body and a fixed cavity; an opening is formed in one side of the cage body, and a fixing cavity is fixedly connected to one side, far away from the opening, of the cage body through a support; the top of the fixed cavity is provided with a light screen, the bottom of the light screen is fixedly connected with an air cylinder through a support, and the end part of the air cylinder is connected with a collecting pipe; a U-shaped elastic sleeve is arranged in the cage body between the fixed cavity and the opening through a support, two sides of the elastic sleeve are fixedly connected with L-shaped connecting rods, the middle parts of the connecting rods are hinged through rotating pins, the tops of the connecting rods are hinged with pull rods, and the pull rods and the connecting rods form a scissor fork mechanism; the top of the elastic sleeve is fixedly connected with an electric push rod, and the top of the electric push rod is hinged with one end of the pull rod, which is far away from the connecting rod; according to the invention, the pig head is aligned and embedded into the fixing cavity, and the flexible clamping and fixing are carried out on the pig head part by matching with the elastic sleeve, so that the shaking of the pig head is reduced, the injury and the acquisition difficulty caused by the severe reaction of the pig to be detected are reduced, and the operation safety is improved.
Description
Technical Field
The invention belongs to the technical field of animal experiment products, and particularly relates to an oral mucus collecting device for PCR detection of hog cholera viruses.
Background
The classical swine fever virus is a pathogen of classical swine fever, and harms pigs and wild pigs, and other animals do not attack the swine fever virus. Hog cholera is an acute, hot and highly contagious infectious disease, and is mainly characterized by systemic hemorrhage, necrosis, infarction and plague bacterial infection caused by high temperature and microvascular degeneration. The swine fever is one of main infectious diseases affecting the healthy production of large-scale pig farms in China, and causes great economic loss to the pig industry.
The swine fever vaccine immunization is an effective means for preventing and controlling swine fever, and the failure of the swine fever vaccine immunization caused by various reasons often occurs. Therefore, the method detects the swine with the swine fever virus by a certain technical means, gradually reduces individuals with continuous infection of the swine fever in the breeding herd by means of eliminating or isolating, strengthening the immunization of the swine fever and the like, and is the most effective method for preventing and controlling the swine fever in a large-scale pig farm (particularly the breeding herd).
The swine fever virus-carrying pig can be judged by checking whether the mucus of the pig carries the swine fever virus. The RT-PCR method is adopted to detect the swine fever virus in the pig saliva, so that the live detection of swine fever virus-carrying pigs in the breeding herd is achieved, and the RT-PCR method is applied to swine fever purification in a large-scale pig farm. Therefore, the research and development of the oral mucus collector of the live pigs are particularly important.
Some technical solutions related to oral mucus collection devices have appeared in the prior art, for example, a chinese patent with application number 2018209071241 discloses a collection device for bovine oral mucus, which includes a collection cylinder, one side of collection tube is connected with the collection pipe that the level set up, the one end that collection tube was kept away from to collection pipe is spherical, the one end that collection tube was kept away from to collection pipe is equipped with the collection hole of even setting, one side parcel that collection tube's outside is close to collection tube has the sponge, sliding connection has the piston on collection tube is close to one side inner wall of collection tube, be fixed with the piston rod that the level set up on the lateral wall of one side of collection tube is kept away from to the intermediate position of piston, it has the first through-hole that link up to open on collection tube's the lateral wall of one side, collection tube's inner wall intermediate position is fixed with the fixed plate of perpendicular setting, open the intermediate position of fixed plate has the second through-hole that link up.
But in stretching into the oral cavity of pig through the collection pipe with collection device among the prior art, later through scraping and carrying out the collection of oral cavity mucus with negative pressure suction, nevertheless because the surprised of pig for the pig head is difficult to the location, causes and gathers the mucus difficulty, and the pig that is surprised still can attack the collection personnel simultaneously, causes the danger.
Therefore, the invention provides an oral mucus collecting device for PCR detection of classical swine fever viruses.
Disclosure of Invention
In order to make up for the defects of the prior art and solve the problems that in the prior art, the collection tube of the collection device extends into the oral cavity of a pig, and then oral mucus is collected by scraping and negative pressure suction, but the pig is frightened, so that the pig head is difficult to position, mucus is difficult to collect, and meanwhile, the frightened pig can attack collection personnel to cause danger, the invention provides the oral mucus collection device for the PCR detection of the classical swine fever virus.
The technical scheme adopted by the invention for solving the technical problems is as follows: the oral mucus collecting device for PCR detection of the classical swine fever virus comprises a cage body and a fixed cavity; an opening is formed in one side of the cage body, a fixing cavity is fixedly connected to one side, far away from the opening, of the cage body through a support, and the fixing cavity is matched with the shape of a pig head to be detected; the top of the fixed cavity is provided with a light screen, and a light hole is formed in the fixed wall at a position corresponding to the top of the light screen; the bottom of the shading plate is fixedly connected with an air cylinder through a support, the end part of the air cylinder is connected with a collecting pipe, and the collecting pipe is connected with a vacuum pump through a pipeline; a U-shaped elastic sleeve is arranged in the cage body between the fixed cavity and the opening through a support, two sides of the elastic sleeve are fixedly connected with L-shaped connecting rods, the middle parts of the connecting rods are hinged through rotating pins, the tops of the connecting rods are hinged with pull rods, and the pull rods and the connecting rods form a scissor fork mechanism; the top of the elastic sleeve is fixedly connected with an electric push rod, and the top of the electric push rod is hinged with one end of the pull rod, which is far away from the connecting rod; when the device works, the collecting pipe of the collecting device extends into the oral cavity of a pig in the prior art, then oral cavity mucus is collected through scraping and negative pressure suction, but the pig head is difficult to position due to frightening of the pig, mucus collection is difficult, and meanwhile the frightened pig can attack collecting personnel to cause danger, at the moment, the device drives the pig to be detected into the cage body, then the opening is closed, so that the pig head in the cage body is aligned to and embedded into the fixed cavity, then the electric push rod drives the connecting rod to rotate, then the connecting rod is matched with the elastic sleeve to flexibly clamp and fix the pig head part, the shaking of the pig head is reduced, the collecting pipe is driven to extend into the oral cavity of the pig head through the electric push rod after the oral cavity is expanded, oral cavity mucus is collected through negative pressure, meanwhile, the severe sight of the pig is shielded and guided through the matching of the light hole and the light screen, the injury and the collection difficulty caused by the anti-reaction of the pig to be detected are reduced, the collection action of gathering the pipe is sheltered from through the light screen, further reduces the surprised struggle of pig, increases the collection efficiency of collection personnel to the oral cavity mucus, avoids receiving the attack simultaneously, increases operational safety.
Preferably, the end part of the cylinder is fixedly connected with a horseshoe-shaped elastic pressure-resistant ring; the collecting pipe is fixedly connected to one end of the pressure ring, which is close to the cylinder; one side of the pressure ring, which is far away from the cylinder, is fixedly connected with a rigid rod, and the periphery of the rigid rod is wrapped with an elastomer; the end part of the collecting pipe is fixedly connected with an elastic flat nozzle; through with behind the fixed chamber of pig head embedding, drive the resistance to pressure ring through electric putter when howling in the mouth of pig and stretch into the oral cavity, cooperation rigidity stick and elastomer carry out certain expansion to the oral cavity, reduce the injury to the oral cavity through the resistance to pressure ring, to the chewing damage of gathering the pipe when reducing the oral cavity closure, increase the operating space of gathering the pipe, further increase the visual area in the oral cavity, increase the degree of depth and the collection time control accuracy that the pipe stretched into in the oral cavity, further increase the collection efficiency of oral cavity mucus, guarantee that oral cavity mucus collection volume is up to standard.
Preferably, the pressure ring is internally provided with a roller arranged in parallel with the rigid rod, the middle part of the roller is sleeved with a rotating shaft, and the roller is rotationally connected with the rotating shaft; the roller is driven to rotate by a hub motor, and two ends of the rotating shaft are connected with the inner periphery of the pressure ring; a group of scraping blades are uniformly distributed on the periphery of the roller; the roller is driven to rotate through the hub motor, and then the mucus of the upper surface of the tongue in the oral cavity is scraped to be gathered by matching with the scraping blade, so that the negative pressure collection efficiency of the collection tube for rolling the oral cavity is further increased, the sample collection time is shortened, and the harm to the pig in the sample collection process is further reduced.
Preferably, a sliding groove is formed in the position, corresponding to the rotating shaft, of the inner periphery of the pressure ring, and a sliding block is connected in the sliding groove in a sliding manner; the rotating shaft is fixedly connected with the sliding block; one side of the roller is fixedly connected with a belt wheel, a pull rope is wound on the belt wheel, and the pull rope is fixedly connected with one end of the sliding groove, which is close to the air cylinder, after being led out by the belt wheel; the sliding block is connected with one end of the sliding chute far away from the air cylinder through a tension spring; through the rotatory in-process of running roller, drive the band pulley and rotate and the rolling stay cord, the cooperation extension spring makes the running roller to the direction removal of being close to the collection pipe, further increases the oral cavity mucus and to the efficiency that is close to the collection pipe position and collects, increases the suction efficiency of collection pipe.
Preferably, the scraping blade is elastic, and a ferromagnetic elastic strip is fixedly connected to the position, close to the outer edge, of the scraping blade; the roller is of a hollow structure, a fixed column is arranged in the roller and fixedly connected with the rotating shaft, and a semicircular magnetic tube is embedded in one side, close to the air cylinder, of the periphery of the fixed column; when moving to one side that is close to the cylinder through the doctor-bar, the magnetic tube attracts the elastic strip for the elastic strip drives the doctor-bar and will be close to the crooked and laminating of running roller outer fringe position, and the mucus that makes the adhesion on the doctor-bar overflows and the one side drippage of top roller near the cylinder flows after receiving the extrusion, further increases the conveying efficiency of doctor-bar to the intracavity mucus.
Preferably, the top of the pressure ring is fixedly connected with a scraper at a position corresponding to the roller, one side of the scraper close to the roller is arranged in an arc shape, and the distance between the inner side of the scraper and the outer edge of the roller is gradually reduced along the direction of the pressure ring close to the rigid rod; one side that is close to the running roller through the scraper blade is the arc and arranges for when the scraper blade was put through the scraper blade, the elasticity strip supported the scraper blade inner wall and drawn close to being close to running roller periphery direction, and the attraction of cooperation magnetic tube makes the laminating degree that increases after the scraper blade warp and running roller periphery, further increases the efficiency of scraping off of adhesion mucus on the scraper blade, increases and gathers pipe collection mucus efficiency.
Preferably, the periphery of the roller is provided with an arc-shaped hole corresponding to the root of the scraping blade, and the arc-shaped hole is communicated with the two sides of the scraping blade; the scraping piece is spirally arranged, and the collecting pipe is positioned at one side where the scraping piece drives oral mucus to collect; communicate the running roller periphery of doctor-bar root both sides through the arc hole, increase the doctor-bar crooked and when laminating near the running roller periphery, remaining mucus is to being close to the efficiency that gathers the pipe direction and spill over between doctor-bar and the running roller, spiral doctor-bar makes the mucus flow to the pipe position of gathering near running roller one end after the mucus is scraped simultaneously, further increase the efficiency of assembling of mucus, spiral doctor-bar is when passing through the scraper blade simultaneously, the doctor-bar outer fringe is in proper order through the scraper blade, reduce the doctor-bar and support the damage that the mucus that the back turned up and cause splashes waste and doctor-bar by the scraper blade.
Preferably, one end of the sliding chute, which is close to the rigid rod, is provided with a sliding cavity, and a sliding plate is connected in the sliding cavity in a sliding manner; the tension spring is fixedly connected with the sliding plate, an annular air bag is fixedly connected between one side of the sliding plate, which is close to the tension spring, and the sliding cavity, the air bag is communicated with a spray pipe arranged at the root part of the scraping blade, which is close to the roller wheel, through an air pipe, and the spray pipe is communicated with the outer surface of the scraping blade through a spray hole and a one-way valve; the slide plate is driven to extrude the air bag when the tension spring receives the pull rope, compressed air generated in the air bag is sprayed out from the spray hole after passing through the spray pipe, the dropping efficiency of adhesive mucus on the scraping blade is further increased, the air pressure in the closed cavity between the scraping blade and the periphery of the roller wheel is increased, and the overflowing confluence efficiency of the adhesive mucus on the scraping blade and the roller wheel is further increased by matching the arc-shaped hole.
Preferably, the air bag is filled with cleaning liquid, and one end of the air pipe penetrates through the inner wall of the air bag and extends to a position below the liquid level of the cleaning liquid; through gathering after accomplishing the back pig head and break away from back through running roller cooperation slider reciprocating sliding in the spout, pour into the washing liquid into the gasbag simultaneously for the washing liquid is through the spray tube blowout, makes running roller intercommunication doctor-bar and scraper blade washed by the washing liquid, increases collection system's cleanliness, reduces the different mucus collection cross mixing that awaits measuring the pig, increases the sample and gathers the purity.
Preferably, one end of the air pipe, which is positioned in the air bag, is fixedly connected with a porous floating ball, and the air pipe is communicated with the floating ball; the length of the air pipe in the air bag is greater than the outer diameter of the air bag, and the air pipe can be bent at will; the end part of the air pipe is always floated on the liquid level of the cleaning liquid through the floating ball, so that the cleaning liquid is mixed and atomized in the air pipe after being mixed with air in the air bag, and then the spraying holes of the mechanism are sprayed out, and the cleaning efficiency of the cleaning liquid on the roller and the scraping blade is further improved.
The invention has the following beneficial effects:
1. the oral mucus collecting device for PCR detection of classical swine fever viruses is characterized in that a pig to be detected is driven into a cage body, an opening is sealed, the pig head in the cage body is aligned and embedded into a fixed cavity, an electric push rod drives a connecting rod to rotate and then is matched with an elastic sleeve to flexibly clamp and fix the pig head part, so that the shaking of the pig head is reduced, an electric push rod drives a collecting pipe to extend into the oral cavity of the pig head after the oral cavity is expanded, oral mucus is collected through negative pressure, the sight line of the pig is shielded and guided through the matching of a light hole and a light shielding plate, the injury and the collection difficulty caused by severe reaction of the pig to be detected are reduced, the collection action of the collecting pipe is shielded through the light shielding plate, the fear of struggling of the pig is further reduced, the collection efficiency of collection personnel on the oral mucus is increased, and the attack is avoided, the operation safety is increased.
2. According to the oral mucus collecting device for PCR detection of CSFV, the slide plate is driven to extrude the air bag when the tension spring is subjected to the pull rope, so that compressed air generated in the air bag is sprayed out from the spray hole after passing through the spray pipe, the dropping efficiency of mucus adhered to the scraping blade is further increased, the air pressure in a closed cavity between the scraping blade and the periphery of the roller is increased, and the overflowing and converging efficiency of the mucus adhered to the scraping blade and the roller is further increased by matching with the arc-shaped hole.
Drawings
The invention will be further explained with reference to the drawings.
FIG. 1 is a perspective view of the present invention;
FIG. 2 is a front view of the present invention;
FIG. 3 is a cross-sectional view taken at A-A in FIG. 2;
FIG. 4 is a cross-sectional view of a fixation lumen in the present invention;
FIG. 5 is a schematic view of the pressure ring and the roller of the present invention;
FIG. 6 is a sectional view of a roller in the present invention;
FIG. 7 is a cross-sectional view of a pressure ring of the present invention;
FIG. 8 is an enlarged view of a portion of FIG. 7 at B;
in the figure: the device comprises a cage body 1, a fixed cavity 11, an opening 12, a light screen 13, a light hole 14, a cylinder 15, a collection pipe 16, an elastic sleeve 17, a connecting rod 18, a pull rod 19, an electric push rod 2, a pressure ring 21, a rigid rod 22, an elastic body 23, a flat nozzle 24, a roller 25, a rotating shaft 26, a scraping blade 27, a sliding chute 28, a sliding block 29, a pull rope 3, a tension spring 31, an elastic strip 32, a fixed column 33, a magnetic pipe 34, a scraping blade 35, an arc hole 36, a sliding cavity 37, a sliding plate 38, an air bag 39, an air pipe 4, a spray pipe 41 and a floating ball 42.
Detailed Description
In order to make the technical means, the creation characteristics, the achievement purposes and the effects of the invention easy to understand, the invention is further described with the specific embodiments.
Example one
As shown in fig. 1 to 7, the oral mucus collection device for PCR detection of swine fever virus according to the present invention comprises a cage 1 and a fixing cavity 11; an opening 12 is formed in one side of the cage body 1, a fixing cavity 11 is fixedly connected to one side, far away from the opening 12, of the cage body 1 through a support, and the fixing cavity 11 is matched with the shape of a pig head to be detected; a light screen 13 is arranged at the top of the fixed cavity 11, and a light hole 14 is formed in the fixed wall at a position corresponding to the top of the light screen 13; the bottom of the shading plate 13 is fixedly connected with an air cylinder 15 through a support, the end part of the air cylinder 15 is connected with a collecting pipe 16, and the collecting pipe 16 is connected with a vacuum pump through a pipeline; a U-shaped elastic sleeve 17 is arranged in the cage body 1 between the fixed cavity 11 and the opening 12 through a support, L-shaped connecting rods 18 are fixedly connected to two sides of the elastic sleeve 17, the middle parts of the connecting rods 18 are hinged through rotating pins, pull rods 19 are hinged to the tops of the connecting rods 18, and the pull rods 19 and the connecting rods 18 form a scissor fork mechanism; the top of the elastic sleeve 17 is fixedly connected with an electric push rod 2, and the top of the electric push rod 2 is hinged with one end of the pull rod 19 far away from the connecting rod 18; when the device works, the collection pipe 16 of the collection device is inserted into the oral cavity of a pig in the prior art, then oral mucus is collected through scraping and negative pressure suction, but the pig head is difficult to position due to frightening of the pig, so that mucus collection is difficult, and meanwhile, the frightened pig can attack collection personnel to cause danger, at the moment, the device drives the pig to be detected into the cage body 1, then the opening 12 is closed, so that the pig head in the cage body 1 is aligned to and embedded into the fixed cavity 11, then the electric push rod 2 drives the connecting rod 18 to rotate, and then the elastic sleeve 17 is matched to flexibly clamp and fix the pig head part, so that the shaking of the pig head is reduced, then the collection pipe 16 is driven to extend into the oral cavity of the pig head through the electric push rod 2 after the oral cavity is expanded, oral mucus is collected through negative pressure, and simultaneously the light shading plate 13 is matched through the light hole 14, so that the sight line of the pig is shielded and guided, the pig that the reduction was waited to detect acutely resists the injury that causes and gathers the degree of difficulty and increase, shelters from the collection action of gathering pipe 16 through light screen 13, further reduces the surprised struggle of pig, increases the collection efficiency of gathering personnel to the oral cavity mucus, avoids receiving the attack simultaneously, increases operational safety.
The end part of the cylinder 15 is fixedly connected with a horseshoe-shaped elastic pressure-resistant ring 21; the collecting pipe 16 is fixedly connected to one end of the pressure ring 21 close to the cylinder 15; one side of the pressure ring 21, which is far away from the air cylinder 15, is fixedly connected with a rigid rod 22, and the periphery of the rigid rod 22 is wrapped with an elastomer 23; an elastic flat nozzle 24 is fixedly connected with the end part of the collection tube 16; through with pig head embedding fixed cavity 11 back, drive pressure ring 21 through electric putter 2 when the howling of pig open the mouth and stretch into in the oral cavity, cooperation rigidity stick 22 and elastomer 23 carry out certain expansion to the oral cavity, reduce the injury to the oral cavity through pressure ring 21, to gathering 16 chewing damage of pipe when reducing the oral cavity closure, increase the operating space who gathers pipe 16, further increase the visible area in the oral cavity, increase and gather the degree of depth and the collection time control precision that pipe 16 stretched into in the oral cavity, further increase the collection efficiency of oral cavity mucus, guarantee that oral cavity mucus collection volume is up to standard.
A roller 25 arranged in parallel with the rigid rod 22 is arranged in the pressure-resistant ring 21, a rotating shaft 26 is sleeved in the middle of the roller 25, and the roller 25 is rotationally connected with the rotating shaft 26; the roller 25 is driven to rotate by a hub motor, and two ends of the rotating shaft 26 are connected with the inner periphery of the pressure ring 21; a group of scraping blades 27 are uniformly distributed on the periphery of the roller 25; the roller 25 is driven to rotate through the hub motor, and then the mucus on the upper surface of the tongue in the oral cavity is scraped and gathered by the scraping blade 27, so that the negative pressure collection efficiency of the collection tube 16 for rolling the oral cavity is further increased, the sample collection time is shortened, and the damage to the pig in the sample collection process is further reduced.
A sliding groove 28 is formed in the inner periphery of the pressure ring 21 and corresponds to the rotating shaft 26, and a sliding block 29 is connected in the sliding groove 28 in a sliding manner; the rotating shaft 26 is fixedly connected with a sliding block 29; one side of the roller 25 is fixedly connected with a belt wheel, a pull rope 3 is wound on the belt wheel, and the pull rope 3 is fixedly connected with one end of the sliding groove 28 close to the air cylinder 15 after being led out by the belt wheel; the sliding block 29 is connected with one end of the sliding groove 28 far away from the air cylinder 15 through a tension spring 31; through the rotatory in-process of running roller 25, drive the band pulley and rotate and the rolling stay cord 3, cooperation extension spring 31 makes running roller 25 to the direction removal of being close to gathering pipe 16, further increases the oral cavity mucus and to the efficiency of being close to gathering pipe 16 position and colleting, increases the suction efficiency of gathering pipe 16.
The scraping blade 27 is elastic, and a ferromagnetic elastic strip 32 is fixedly connected to the scraping blade 27 near the outer edge; the roller 25 is of a hollow structure, a fixing column 33 is arranged in the roller 25, the fixing column 33 is fixedly connected with the rotating shaft 26, and a semicircular magnetic tube 34 is embedded in one side, close to the air cylinder 15, of the periphery of the fixing column 33; when the scraping blade 27 moves to one side close to the cylinder 15, the magnetic tube 34 attracts the elastic strip 32, so that the elastic strip 32 drives the scraping blade 27 to bend and attach to the position close to the outer edge of the roller 25, the sticky liquid adhered to the scraping blade 27 overflows after being extruded and drips and flows on one side of the roller 25 close to the cylinder 15, and the transportation efficiency of the scraping blade 27 on the sticky liquid in the oral cavity is further improved.
The top of the pressure ring 21 is fixedly connected with a scraper 35 at a position corresponding to the roller 25, one side of the scraper 35 close to the roller 25 is arranged in an arc shape, and the distance between the inner side of the scraper 35 and the outer edge of the roller 25 is gradually reduced along the direction of the pressure ring 21 close to the rigid rod 22; one side of being close to running roller 25 through scraper blade 35 is the arc nature and arranges for when doctor-bar 27 passes through scraper blade 35 position, elastic strip 32 supports scraper blade 35 inner wall and draws close to being close to running roller 25 periphery direction, and the attraction of cooperation magnetic tube 34 makes the laminating degree that increases doctor-bar 27 after warping and running roller 25 periphery, further increases the efficiency of scraping off of adhesion mucus on doctor-bar 27, increases and gathers pipe 16 and collect mucus efficiency.
The periphery of the roller 25 is provided with an arc-shaped hole 36 corresponding to the root of the scraping blade 27, and the arc-shaped hole 36 is communicated with two sides of the scraping blade 27; the scraping blade 27 is spirally arranged, and the collecting tube 16 is positioned at one side where the scraping blade 27 drives oral mucus to collect; the peripheries of the rollers 25 on two sides of the root of the scraping blade 27 are communicated through the arc-shaped holes 36, when the scraping blade 27 is bent and attached to the periphery close to the rollers 25, the efficiency of overflowing of residual mucus between the scraping blade 27 and the rollers 25 towards the direction close to the collecting pipe 16 is increased, meanwhile, the spiral scraping blade 27 enables the mucus to flow towards the position, close to the collecting pipe 16 at one end of the rollers 25, of the collecting pipe after being scraped, the mucus is matched with the scraper 35 to flow, the gathering efficiency of the mucus is further increased, meanwhile, when the spiral scraping blade 27 passes through the scraper 35, the outer edge of the scraping blade 27 sequentially passes through the scraper 35, and mucus splashing waste and damage of the scraping blade 27 caused by outward turning of the scraping blade 27 after being propped by the scraper 35 are reduced.
One end of the sliding chute 28 close to the rigid rod 22 is provided with a sliding cavity 37, and a sliding plate 38 is connected in the sliding cavity 37 in a sliding manner; the tension spring 31 is fixedly connected with the sliding plate 38, an annular air bag 39 is fixedly connected between one side of the sliding plate 38 close to the tension spring 31 and the sliding cavity 37, the air bag 39 is communicated with a spray pipe 41 arranged at the root part of the scraping blade 27 close to the roller 25 through an air pipe 4, and the spray pipe 41 is communicated with the outer surface of the scraping blade 27 through a spray hole and a one-way valve; when the tension spring 31 is subjected to the pull rope 3, the sliding plate 38 is driven to extrude the air bag 39, compressed air generated in the air bag 39 is sprayed out from the spraying hole after passing through the spraying pipe 41, the dropping efficiency of mucus adhered to the scraping blade 27 is further improved, meanwhile, the air pressure in a closed cavity between the scraping blade 27 and the periphery of the roller 25 is increased, and the overflowing and converging efficiency of the mucus adhered to the scraping blade 27 and the roller 25 is further improved by matching with the arc-shaped hole 36.
Example two
As shown in fig. 8, a first comparative example, in which another embodiment of the present invention is: the air bag 39 is filled with cleaning liquid, and one end of the air pipe 4 penetrates through the inner wall of the air bag 39 and extends to a position below the liquid level of the cleaning liquid; through gathering the pig head and breaking away from back through running roller 25 cooperation slider 29 reciprocal slip in spout 28 after accomplishing, pour into the washing liquid into gasbag 39 simultaneously for the washing liquid is spout through spray tube 41, makes running roller 25 intercommunication doctor-bar 27 and scraper blade 35 washed by the washing liquid, increases collection device's cleanliness, reduces the different mucus collection cross-mixing that awaits measuring the pig, increases the sample and gathers the purity.
One end of the air pipe 4 positioned in the air bag 39 is fixedly connected with a porous floating ball 42, and the air pipe 4 is communicated with the floating ball 42; the length of the air pipe 4 in the air bag 39 is larger than the outer diameter of the air bag 39, and the air pipe 4 can be bent at will; the floating ball 42 makes the end of the air tube 4 always float on the liquid level of the cleaning liquid, so that the cleaning liquid is mixed and atomized in the air tube 4 after being mixed with the air in the air bag 39, and then is sprayed out through the spray holes, and the cleaning efficiency of the cleaning liquid on the roller 25 and the scraper 27 is further improved.
When in work, the invention drives the pig to be detected into the cage body 1, then the opening 12 is closed, the pig head in the cage body 1 is aligned and embedded into the fixed cavity 11, then the electric push rod 2 drives the connecting rod 18 to rotate, then the elastic sleeve 17 is matched to flexibly clamp and fix the pig head part, the shaking of the pig head is reduced, then the electric push rod 2 drives the collecting pipe 16 to extend into the oral cavity of the pig head after the oral cavity is expanded, oral mucus is collected through negative pressure, meanwhile, the light hole 14 is matched with the light screen 13, so that the sight of the pig is shielded and guided, the injury and the acquisition difficulty caused by the severe resistance of the pig to be detected are reduced, the light screen 13 is used for shielding the collecting action of the collecting tube 16, so that the frightened struggle of the pig is further reduced, the collecting efficiency of the collecting personnel on oral mucus is increased, meanwhile, the attack is avoided, and the operation safety is improved; after the pig head is embedded into the fixed cavity 11, the electric push rod 2 drives the pressure ring 21 to extend into the oral cavity when a pig howls opens the mouth, the rigid rod 22 and the elastic body 23 are matched to expand the oral cavity to a certain extent, the damage to the oral cavity is reduced through the pressure ring 21, the chewing damage to the collecting pipe 16 when the oral cavity is closed is reduced, the operating space of the collecting pipe 16 is increased, the visible area in the oral cavity is further increased, the depth and collecting time control precision of the collecting pipe 16 extending into the oral cavity are increased, the collecting efficiency of oral mucus is further increased, and the collecting quantity of the oral mucus is guaranteed to reach the standard; the roller 25 is driven to rotate by the hub motor, and then the scraping blade 27 is matched to scrape and collect mucus on the upper surface of the tongue in the oral cavity, so that the negative pressure collection efficiency of the collection tube 16 for grinding the oral cavity is further increased, the sample collection time is reduced, and the damage to pigs in the sample collection process is further reduced; in the rotating process of the roller 25, the belt wheel is driven to rotate and the pull rope 3 is wound, the roller 25 is driven to move towards the direction close to the collection pipe 16 by matching with the tension spring 31, the collection efficiency of mucus in the oral cavity towards the position close to the collection pipe 16 is further increased, and the suction efficiency of the collection pipe 16 is increased; when the scraping blade 27 moves to one side close to the cylinder 15, the magnetic tube 34 attracts the elastic strip 32, so that the elastic strip 32 drives the scraping blade 27 to bend and attach the position close to the outer edge of the roller 25, and the mucus adhered to the scraping blade 27 overflows after being extruded and drips and flows on one side of the roller 25 close to the cylinder 15, thereby further increasing the transportation efficiency of the scraping blade 27 on the mucus in the oral cavity; one side of the scraping plate 35 close to the roller 25 is arranged in an arc shape, so that when the scraping plate 27 passes through the position of the scraping plate 35, the elastic strip 32 props against the inner wall of the scraping plate 35 and is close to the periphery of the roller 25, the adhesion degree of the deformed scraping plate 27 and the periphery of the roller 25 is increased by matching with the attraction of the magnetic tube 34, the scraping efficiency of the sticky liquid adhered on the scraping plate 27 is further increased, and the efficiency of the collection tube 16 for collecting the sticky liquid is increased; the peripheries of the rollers 25 at two sides of the root of the scraping blade 27 are communicated through the arc-shaped holes 36, so that the efficiency of overflowing residual mucus between the scraping blade 27 and the rollers 25 to the direction close to the collecting pipe 16 when the scraping blade 27 is bent and attached to the periphery close to the rollers 25 is increased, meanwhile, the spiral scraping blade 27 enables the mucus to flow to the position of the collecting pipe 16 close to one end of the rollers 25 by matching with the scraping blade 35 after being scraped, the gathering efficiency of the mucus is further increased, meanwhile, when the spiral scraping blade 27 passes through the scraping blade 35, the outer edge of the scraping blade 27 sequentially passes through the scraping blade 35, and the mucus splashing waste and the damage of the scraping blade 27 caused by the outward turning of the scraping blade 27 after being propped by the scraping blade 35 are reduced; when the tension spring 31 is subjected to the pull rope 3, the sliding plate 38 is driven to extrude the air bag 39, compressed air generated in the air bag 39 is sprayed out from the spraying hole after passing through the spraying pipe 41, the dropping efficiency of mucus adhered to the scraping blade 27 is further improved, meanwhile, the air pressure in a closed cavity between the scraping blade 27 and the periphery of the roller 25 is increased, and the overflowing and converging efficiency of the mucus adhered to the scraping blade 27 and the roller 25 is further improved by matching with the arc-shaped hole 36.
The front, the back, the left, the right, the upper and the lower are all based on figure 1 in the attached drawings of the specification, according to the standard of the observation angle of a person, the side of the device facing an observer is defined as the front, the left side of the observer is defined as the left, and the like.
In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate orientations or positional relationships based on those shown in the drawings, and are used merely for convenience in describing the present invention and for simplifying the description, but do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the scope of the present invention.
The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (10)
1. The utility model provides a classical swine fever virus PCR detects uses oral cavity mucus collection system which characterized in that: comprises a cage body (1) and a fixed cavity (11); an opening (12) is formed in one side of the cage body (1), a fixing cavity (11) is fixedly connected to one side, far away from the opening (12), of the cage body (1) through a support, and the fixing cavity (11) is matched with the shape of a pig head to be detected; a light screen (13) is arranged at the top of the fixed cavity (11), and a light hole (14) is formed in the fixed wall at a position corresponding to the top of the light screen (13); the bottom of the shading plate (13) is fixedly connected with an air cylinder (15) through a support, the end part of the air cylinder (15) is connected with a collection pipe (16), and the collection pipe (16) is connected with a vacuum pump through a pipeline; a U-shaped elastic sleeve (17) is arranged in the cage body (1) between the fixed cavity (11) and the opening (12) through a support, two sides of the elastic sleeve (17) are fixedly connected with L-shaped connecting rods (18), the middle parts of the connecting rods (18) are hinged through rotating pins, the top parts of the connecting rods (18) are hinged with pull rods (19), and the pull rods (19) and the connecting rods (18) form a scissor fork mechanism; the top of the elastic sleeve (17) is fixedly connected with an electric push rod (2), and the top of the electric push rod (2) is hinged with one end of the pull rod (19) far away from the connecting rod (18).
2. The oral mucus collection device for PCR detection of CSFV according to claim 1, wherein: the end part of the cylinder (15) is fixedly connected with a horseshoe-shaped elastic pressure-resistant ring (21); the collecting pipe (16) is fixedly connected to one end of the pressure ring (21) close to the cylinder (15); one side of the pressure ring (21) far away from the air cylinder (15) is fixedly connected with a rigid rod (22), and the periphery of the rigid rod (22) is wrapped with an elastomer (23); the end part of the collecting pipe (16) is fixedly connected with an elastic flat nozzle (24).
3. The oral mucus collection device for PCR detection of CSFV according to claim 2, wherein: a roller (25) which is arranged in parallel with the rigid rod (22) is arranged in the pressure ring (21), a rotating shaft (26) is sleeved in the middle of the roller (25), and the roller (25) is rotationally connected with the rotating shaft (26); the roller (25) is driven to rotate by a hub motor, and two ends of the rotating shaft (26) are connected with the inner periphery of the pressure ring (21); a group of scraping blades (27) are uniformly distributed on the periphery of the roller (25).
4. The oral mucus collection device for PCR detection of CSFV according to claim 3, wherein: a sliding groove (28) is formed in the inner periphery of the pressure ring (21) and corresponds to the rotating shaft (26), and a sliding block (29) is connected in the sliding groove (28) in a sliding manner; the rotating shaft (26) is fixedly connected with the sliding block (29); one side of the roller (25) is fixedly connected with a belt wheel, a pull rope (3) is wound on the belt wheel, and the pull rope (3) is fixedly connected with one end, close to the cylinder (15), of the sliding groove (28) after being led out through the belt wheel; the sliding block (29) is connected with one end, far away from the air cylinder (15), of the sliding groove (28) through a tension spring (31).
5. The oral mucus collection device for PCR detection of CSFV according to claim 4, wherein: the scraping blade (27) is elastic, and a ferromagnetic elastic strip (32) is fixedly connected to the position, close to the outer edge, of the scraping blade (27); running roller (25) are hollow structure, and are equipped with fixed column (33) in running roller (25), and fixed column (33) link firmly with pivot (26), and fixed column (33) periphery is close to one side of cylinder (15) and inlays and have semicircular magnetron (34).
6. The oral mucus collection device for PCR detection of CSFV according to claim 3, wherein: the top of the pressure ring (21) is fixedly connected with a scraper (35) at a position corresponding to the roller (25), one side of the scraper (35) close to the roller (25) is arranged in an arc shape, and the distance between the inner side of the scraper (35) and the outer edge of the roller (25) is gradually reduced along the direction of the pressure ring (21) close to the rigid rod (22).
7. The oral mucus collection device for PCR detection of CSFV according to claim 3, wherein: the periphery of the roller (25) is provided with an arc-shaped hole (36) at a position corresponding to the root of the scraping blade (27), and the arc-shaped hole (36) is communicated with the two sides of the scraping blade (27); the scraping blade (27) is spirally arranged, and the collecting pipe (16) is positioned at one side where the scraping blade (27) drives oral mucus to collect.
8. The oral mucus collection device for PCR detection of CSFV according to claim 4, wherein: one end of the sliding chute (28) close to the rigid rod (22) is provided with a sliding cavity (37), and a sliding plate (38) is connected in the sliding cavity (37) in a sliding manner; extension spring (31) and slide (38) link firmly, have linked firmly annular gasbag (39) between slide (38) one side and sliding chamber (37) that are close to extension spring (31), and spout (41) intercommunication that the root position that gasbag (39) are close to running roller (25) through trachea (4) and doctor-bar (27) was seted up, and spout (41) are through orifice and check valve and doctor-bar (27) surface intercommunication.
9. The oral mucus collection device for PCR detection of CSFV according to claim 8, wherein: the air bag (39) is filled with cleaning liquid, and one end of the air pipe (4) penetrates through the inner wall of the air bag (39) and extends to a position below the liquid level of the cleaning liquid.
10. The oral mucus collection device for PCR detection of CSFV according to claim 8, wherein: one end of the air pipe (4) positioned in the air bag (39) is fixedly connected with a porous floating ball (42), and the air pipe (4) is communicated with the floating ball (42); the length of the air pipe (4) in the air bag (39) is larger than the outer diameter of the air bag (39), and the air pipe (4) can be bent at will.
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| CN202111111297.5A CN113951937B (en) | 2021-09-23 | 2021-09-23 | Oral mucus collection device for classical swine fever virus PCR detection |
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| CN202111111297.5A CN113951937B (en) | 2021-09-23 | 2021-09-23 | Oral mucus collection device for classical swine fever virus PCR detection |
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| CN113951937B CN113951937B (en) | 2024-02-09 |
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