CN113943644A - Microorganism inspection extractor - Google Patents

Microorganism inspection extractor Download PDF

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Publication number
CN113943644A
CN113943644A CN202111369370.9A CN202111369370A CN113943644A CN 113943644 A CN113943644 A CN 113943644A CN 202111369370 A CN202111369370 A CN 202111369370A CN 113943644 A CN113943644 A CN 113943644A
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Prior art keywords
box
plate
rotating
extractor
fixedly arranged
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Withdrawn
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CN202111369370.9A
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Chinese (zh)
Inventor
王雪
李江涛
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Individual
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Priority to CN202111369370.9A priority Critical patent/CN113943644A/en
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/38Caps; Covers; Plugs; Pouring means
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Clinical Laboratory Science (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention discloses a microorganism detection extractor which comprises a detection box, wherein a servo motor is fixedly arranged in the detection box, a rotating box is fixedly arranged on a driving end of the servo motor, an oscillating mechanism is arranged in the rotating box, a plurality of liquid discharging pipes are fixedly arranged on the rotating box, a top plate is fixedly arranged on the detection box, two liquid taking pipes are arranged on the top plate through two rotating mechanisms, extraction pipes are fixedly arranged at the lower parts of the two liquid taking pipes and are positioned in the rotating box, a liquid inlet pipe is fixedly arranged on the top plate, and a sealing cover is arranged on the liquid inlet pipe in a threaded manner. Has the advantages that: the invention can vibrate the culture solution, thereby uniformly distributing the microorganisms, increasing the sampling uniformity, carrying out rapid multi-position sampling, having no external pollution during sampling and higher inspection accuracy.

Description

Microorganism inspection extractor
Technical Field
The invention relates to the technical field of microbial detection, in particular to a microbial detection extractor.
Background
When an existing laboratory is used for carrying out a microorganism culture observation experiment, a culture solution is usually injected into an extractor firstly, then microorganisms are put into the culture solution to culture the microorganisms, and the culture solution in the extractor is extracted and collected at any time in the culture process, so that the observation and the inspection of the growth condition of the microorganisms are facilitated;
the prior extractor usually has the following defects in the using process:
1. when the existing device is used for extracting microorganisms, the culture solution cannot be vibrated, so that the microorganisms can be accumulated in partial areas in the culture solution and cannot be uniformly distributed, and the number of the microorganisms in the culture solution taken out is too large or too small, namely, the inspection result is influenced;
2. when the existing device is used for extracting microorganisms, the whole device is usually opened for extraction, and microorganisms or impurities in external air enter a culture solution in the device in the extraction process in such a way, so that the inspection result is influenced;
3. the existing device cannot rapidly complete the collection and separation of the culture solution and flexibly collect the culture solution in different areas in the extractor, the collection efficiency is low, the collection effect is poor, and certain limitations are realized;
therefore, it is desirable to design a microbiological examination extractor to solve the above problems.
Disclosure of Invention
The invention aims to solve the problems in the prior art and provides a microbiological examination extractor.
In order to achieve the purpose, the invention adopts the following technical scheme:
a microorganism detection extractor comprises a detection box, wherein a servo motor is fixedly installed in the detection box, a rotating box is fixedly installed on a driving end of the servo motor, a vibration mechanism is installed in the rotating box, a plurality of liquid discharging pipes are fixedly installed on the detection box, a top plate is fixedly installed on the detection box, two liquid taking pipes are installed on the top plate through two rotating mechanisms, extraction pipes are fixedly installed at the lower parts of the two liquid taking pipes and are located in the rotating box, a liquid feeding pipe is fixedly installed on the top plate, and a sealing cover is installed on the liquid feeding pipe in a threaded mode;
two all install the movable plate through slide mechanism in getting the liquid pipe, and equal slidable mounting has the control lever between every movable plate and the corresponding liquid taking pipe, every equal fixed mounting has a plurality of reset spring, two between movable plate and the corresponding liquid taking pipe equal fixed mounting has the collecting pipe, two on the liquid taking pipe equal fixed mounting has the current-limiting plate in the extraction pipe, and all installs current-limiting mechanism in two current-limiting plates.
In foretell microbial detection extractor, vibrate the mechanism and include the toper piece of fixed mounting in rotating the box, fixed mounting has a plurality of mounts in rotating the box, every all rotate on the mount and install from the pivot that restores to the throne, and every from the pivot that restores to the throne equal fixed mounting have the shake board.
In foretell microbial detection extractor, every all seted up a plurality of vibrations holes on shaking the board, fixed mounting has the separation ring in the test box, and servo motor is located the separation ring.
In foretell microbial detection extractor, slewing mechanism is including seting up the semi-ring channel in the roof, there is the turning block through the cooperation slidable mounting of spacing groove and stopper in the semi-ring channel, and liquid intaking pipe fixed mounting is on the turning block.
In foretell microbial detection extractor, slide mechanism includes two trapezoidal sliders of fixed mounting on the movable plate, it has two trapezoidal spouts to open in the liquid intaking pipe, and two trapezoidal sliders block respectively and install in two trapezoidal spouts.
In foretell microbial detection extractor, current-limiting mechanism is including seting up fixed slot, the intercommunicating pore in the current-limiting plate, and fixed slot and intercommunicating pore are linked together, there is the lifter plate through a plurality of compression spring fixed mounting in the fixed slot, and fixed mounting has the intercommunication post with intercommunicating pore matched with on the lifter plate.
In the microorganism inspection extractor, the lifting plate is fixedly provided with a connecting rod, and the connecting rod is fixedly provided with a rubber sealing plate matched with the fixing groove.
Compared with the prior art, the invention has the advantages that:
1: through setting up the feed liquor pipe, put into the rotation box in the inspection box through the feed liquor pipe with the culture solution earlier, pour into the microorganism into the rotation box through the feed liquor pipe again, at last will seal the lid screw in the feed liquor pipe, can carry out the whole of inspection box, feed liquor pipe sealed.
2: through setting up slewing mechanism, the turning block in the slewing mechanism can be at the internal rotation of semicircular groove to can drive the liquid intaking pipe level on it and rotate, can rotate the interior multiposition culture solution sample of box, thereby multiplicable sample scope.
3: through setting up the oscillation mechanism, when taking a sample, can start servo motor and make it drive and rotate the box and rotate, rotate the box and rotate and can drive culture solution in it and rotate, the culture solution rotates and can contact the collision with the toper piece and oscillation board promptly to can accelerate the mobility of culture solution, make microorganism in it can not pile up, make microorganism evenly distributed in the culture solution, increase the accuracy nature of sample.
4: through setting up control lever and movable plate, after the position adjustment of extraction tube finished, operating personnel can hold the control lever and pull up, can drive the movable plate and shift up when the control lever shifts up, can inhale the culture solution in the rotation box through the extraction tube when the movable plate shifts up and get the liquid pipe in, can accomplish the sample, push down the movable plate after the sample is accomplished and can pass through the collecting pipe extrusion with getting the culture solution in the liquid pipe promptly, can carry out subsequent inspection work.
In conclusion, the invention can shake the culture solution, thereby uniformly distributing the microorganisms, increasing the sampling uniformity, carrying out rapid multi-position sampling, avoiding external pollution during sampling and having higher inspection accuracy.
Drawings
Embodiments of the invention are described in further detail below with reference to the attached drawing figures, wherein:
FIG. 1 is a schematic structural view of a microorganism testing extractor according to the present invention;
FIG. 2 is a cross-sectional view of the test cassette of FIG. 1 with a portion of the attachment structure thereon;
FIG. 3 is an enlarged view of the rotary case, the top plate and a portion of the connection structure therebetween shown in FIG. 2;
FIG. 4 is an enlarged view of the connection structure of the fixing frame and the upper portion of the fixing frame in FIG. 2;
FIG. 5 is an enlarged view of the fluid tube of FIG. 2 with the attachment structure thereon;
FIG. 6 is a sectional view showing the inner structure of the liquid take-out tube of FIG. 5;
fig. 7 is a schematic view of the internal structure of the restrictor plate of fig. 6.
In the figure: the device comprises a test box 1, a top plate 2, a liquid inlet pipe 3, a semi-annular groove 4, a liquid taking pipe 5, a liquid discharging pipe 6, a control rod 7, a blocking ring 8, a servo motor 9, a rotating box 10, a conical block 11, an extraction pipe 12, a fixed frame 13, a liquid discharging pipe 14, a self-resetting rotating shaft 15, an oscillating plate 16, a collecting pipe 17, a movable plate 18, a return spring 19, a flow limiting plate 20, a fixed groove 21, a lifting plate 22, a communication hole 23, a compression spring 24 and a rubber sealing plate 25.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are merely for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention.
Referring to fig. 1-4, a microorganism detection extractor comprises a detection box 1, wherein a servo motor 9 is fixedly installed in the detection box 1, a rotating box 10 is fixedly installed on a driving end of the servo motor 9, a vibration mechanism is installed in the rotating box 10, a plurality of liquid discharging pipes 14 are fixedly installed on the rotating box 10, a plurality of liquid discharging pipes 6 are fixedly installed on the detection box 1, and a top plate 2 is fixedly installed on the detection box 1;
the following points are notable:
1. install two liquid intaking pipes 5 through two slewing mechanism on roof 2, the equal fixed mounting in lower part of two liquid intaking pipes 5 has extraction pipe 12, and two extraction pipes 12 all are located and rotate box 10, and fixed mounting has feed liquor pipe 3 on roof 2, and threaded mounting has sealed lid on the feed liquor pipe 3.
2. Put into the rotation box 10 in the inspection box 1 through feed liquor pipe 3 earlier the culture solution, again with microorganism also through feed liquor pipe 3 injection rotation box 10 in, at last will seal the lid screw in feed liquor pipe 3 in, can carry out the whole of inspection box 1, feed liquor pipe 3 sealed to avoid impurity in the outside air and microorganism to get into in the inspection box 1.
3. Slewing mechanism has the turning block including offering the semicircular groove 4 in roof 2, and there is the cooperation slidable mounting of spacing groove and stopper in the semicircular groove 4, and liquid taking pipe 5 fixed mounting on the turning block, and the turning block can be at the 4 internal rotations of semicircular groove to can drive the horizontal rotation of liquid taking pipe 5 on it, can rotate the culture solution sample of the interior multiposition of box 10, thereby multiplicable sample scope.
4. The oscillating mechanism comprises a conical block 11 fixedly arranged in a rotating box 10, a plurality of fixing frames 13 are fixedly arranged in the rotating box 10, a self-resetting rotating shaft 15 is rotatably arranged on each fixing frame 13, and an oscillating plate 16 is fixedly arranged on each self-resetting rotating shaft 15;
when taking a sample, can start servo motor 9 and make it drive and rotate box 10 and rotate, rotate box 10 and rotate and can drive the culture solution rotation in it promptly, the culture solution rotates and can contact the collision with toper piece 11 and shock board 16 promptly to can accelerate the mobility of culture solution, make the microorganism in it can not pile up, make microorganism evenly distributed in the culture solution, increase the accuracy nature of sample.
5. Each vibrating plate 16 is provided with a plurality of vibrating holes, and the contact area and range of the vibrating plate 16 and the culture solution can be increased by the vibrating holes, so that the vibrating fluidity of the culture solution can be further increased.
6. After the culture solution in the rotating box 10 is sampled, the operator can open the liquid dropping pipe 14 on the rotating box 10, and at this time, the culture solution can fall into the testing box 1 through the liquid dropping pipe 14 and can be discharged to the outside of the testing box 1 through the liquid discharging pipes 6.
7. Fixed mounting has separation ring 8 in inspection box 1, and servo motor 9 is located separation ring 8, and separation ring 8 plays the effect of separation, can prevent that the culture solution from falling into and contacting with servo motor 9 behind the bottom of inspection box 1 to prevent to pile up the culture solution on servo motor 9.
Referring to fig. 5-7, moving plates 18 are mounted in the two liquid taking tubes 5 through sliding mechanisms, a control rod 7 is slidably mounted between each moving plate 18 and the corresponding liquid taking tube 5, a plurality of return springs 19 are fixedly mounted between each moving plate 18 and the corresponding liquid taking tube 5, collecting tubes 17 are fixedly mounted on the two liquid taking tubes 5, flow limiting plates 20 are fixedly mounted in the two extraction tubes 12, and flow limiting mechanisms are mounted in the two flow limiting plates 20;
the following points are notable:
1. the sliding mechanism comprises two trapezoidal sliding blocks fixedly mounted on the moving plate 18, two trapezoidal sliding grooves are formed in the liquid taking pipe 5, the two trapezoidal sliding blocks are respectively clamped and mounted in the two trapezoidal sliding grooves, and the sliding mechanism is used for enabling the moving plate 18 to only slide up and down in the liquid taking pipe 5 without deviating and separating from the liquid taking pipe 5.
2. After the position of the extraction tube 12 is adjusted, an operator can hold the control rod 7 and pull the control rod up, the control rod 7 drives the moving plate 18 to move up when moving up, the moving plate 18 sucks the culture solution in the rotating box 10 into the liquid taking tube 5 through the extraction tube 12 when moving up, sampling can be completed, and after sampling is completed, the culture solution in the liquid taking tube 5 can be pressed out through the collecting tube 17 by pressing down the moving plate 18, and subsequent inspection work can be performed.
3. The flow limiting mechanism comprises a fixing groove 21 and a communicating hole 23 which are arranged in the flow limiting plate 20, the fixing groove 21 is communicated with the communicating hole 23, a lifting plate 22 is fixedly arranged in the fixing groove 21 through a plurality of compression springs 24, and a communicating column matched with the communicating hole 23 is fixedly arranged on the lifting plate 22;
4. when the moving plate 18 moves up to suck the culture solution, the connecting column and the lifting plate 22 move up at the moment, the culture solution enters the liquid taking tube 5 through the communicating hole 23 and the fixing groove 21, after the liquid taking is completed, the lifting plate 22 moves down to reset under the resetting action of the compression spring 24, so that the connecting column is driven to return to the communicating hole 23, the communicating hole 23 can be sealed, when the moving plate 18 moves down, the lifting plate 22 moves down to drive the connecting column to move down, the communicating hole 23 cannot be in a communicating state, and the culture solution in the liquid taking tube 5 cannot flow back through the flow limiting plate 20 and is only discharged through the collecting tube 17.
5. Fixed mounting has the connecting rod on the lifter plate 22, and fixed mounting has the rubber seal plate 25 with fixed slot 21 matched with on the connecting rod, and when lifter plate 22 moved down, rubber seal plate 25 can move down and get into fixed slot 21 simultaneously promptly, can carry out the shutoff to fixed slot 21 promptly, makes the culture solution in the liquid intaking pipe 5 can not get into in the fixed slot 21.
Further, unless otherwise specifically stated or limited, the above-described fixed connection is to be understood in a broad sense, and may be, for example, welded, glued, or integrally formed as is conventional in the art.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.

Claims (7)

1. A microorganism detection extractor comprises a detection box (1), and is characterized in that a servo motor (9) is fixedly arranged in the detection box (1), a rotating box (10) is fixedly arranged on the driving end of the servo motor (9), a vibration mechanism is arranged in the rotating box (10), a plurality of liquid discharging pipes (14) are fixedly arranged on the rotating box (10), a plurality of liquid discharging pipes (6) are fixedly arranged on the inspection box (1), the inspection box (1) is fixedly provided with a top plate (2), the top plate (2) is provided with two liquid taking pipes (5) through two rotating mechanisms, the lower parts of the two liquid taking pipes (5) are fixedly provided with extraction pipes (12), the two extraction pipes (12) are positioned in the rotating box (10), the liquid inlet pipe (3) is fixedly arranged on the top plate (2), and the liquid inlet pipe (3) is provided with a sealing cover in a threaded manner;
two all install movable plate (18) through slide mechanism in liquid taking pipe (5), and equal slidable mounting has control lever (7), every between every movable plate (18) and corresponding liquid taking pipe (5) equal fixed mounting has a plurality of reset spring (19), two between movable plate (18) and corresponding liquid taking pipe (5) equal fixed mounting has collecting pipe (17), two on liquid taking pipe (5) equal fixed mounting has current-limiting plate (20) in extraction pipe (12), and all installs current-limiting mechanism in two current-limiting plate (20).
2. The microorganism inspection extractor as claimed in claim 1, wherein the oscillating mechanism comprises a conical block (11) fixedly installed in a rotating box (10), a plurality of fixing frames (13) are fixedly installed in the rotating box (10), each fixing frame (13) is rotatably installed with a self-resetting rotating shaft (15), and each self-resetting rotating shaft (15) is fixedly installed with an oscillating plate (16).
3. The microorganism inspection extractor as claimed in claim 2, wherein each of the oscillation plates (16) has a plurality of oscillation holes, a blocking ring (8) is fixedly installed in the inspection box (1), and the servo motor (9) is located in the blocking ring (8).
4. The microorganism inspection extractor as claimed in claim 1, wherein the rotating mechanism comprises a semi-annular groove (4) formed in the top plate (2), a rotating block is slidably mounted in the semi-annular groove (4) through the matching of a limiting groove and a limiting block, and the liquid taking pipe (5) is fixedly mounted on the rotating block.
5. The microorganism testing extractor as claimed in claim 1, wherein the sliding mechanism comprises two trapezoidal sliding blocks fixedly mounted on the moving plate (18), two trapezoidal sliding grooves are formed in the liquid taking tube (5), and the two trapezoidal sliding blocks are respectively snap-mounted in the two trapezoidal sliding grooves.
6. The microorganism inspection extractor according to claim 1, wherein the flow restriction mechanism comprises a fixing groove (21) and a communication hole (23) which are arranged in the flow restriction plate (20), the fixing groove (21) is communicated with the communication hole (23), a lifting plate (22) is fixedly arranged in the fixing groove (21) through a plurality of compression springs (24), and a communication column which is matched with the communication hole (23) is fixedly arranged on the lifting plate (22).
7. The microorganism testing extractor as claimed in claim 6, wherein the lifting plate (22) is fixedly installed with a connecting rod, and the connecting rod is fixedly installed with a rubber sealing plate (25) which is matched with the fixing groove (21).
CN202111369370.9A 2021-11-18 2021-11-18 Microorganism inspection extractor Withdrawn CN113943644A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202111369370.9A CN113943644A (en) 2021-11-18 2021-11-18 Microorganism inspection extractor

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202111369370.9A CN113943644A (en) 2021-11-18 2021-11-18 Microorganism inspection extractor

Publications (1)

Publication Number Publication Date
CN113943644A true CN113943644A (en) 2022-01-18

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Application Number Title Priority Date Filing Date
CN202111369370.9A Withdrawn CN113943644A (en) 2021-11-18 2021-11-18 Microorganism inspection extractor

Country Status (1)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115595245A (en) * 2022-11-10 2023-01-13 延边大学(Cn) Medical clinical microbiological detection sampling device

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115595245A (en) * 2022-11-10 2023-01-13 延边大学(Cn) Medical clinical microbiological detection sampling device
CN115595245B (en) * 2022-11-10 2023-06-27 延边大学 Medical clinical microorganism detection sampling device

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Application publication date: 20220118

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