CN113933290A - Chemical indicator card for detecting concentration of quaternary ammonium salt disinfectant, mother solution and preparation method of chemical indicator card - Google Patents
Chemical indicator card for detecting concentration of quaternary ammonium salt disinfectant, mother solution and preparation method of chemical indicator card Download PDFInfo
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- CN113933290A CN113933290A CN202111116453.7A CN202111116453A CN113933290A CN 113933290 A CN113933290 A CN 113933290A CN 202111116453 A CN202111116453 A CN 202111116453A CN 113933290 A CN113933290 A CN 113933290A
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- 150000003242 quaternary ammonium salts Chemical class 0.000 title claims abstract description 104
- 239000000126 substance Substances 0.000 title claims abstract description 85
- 239000000645 desinfectant Substances 0.000 title claims abstract description 79
- 239000010413 mother solution Substances 0.000 title claims abstract description 25
- 238000002360 preparation method Methods 0.000 title claims abstract description 17
- 239000002250 absorbent Substances 0.000 claims abstract description 44
- 230000002745 absorbent Effects 0.000 claims abstract description 43
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 33
- 239000000243 solution Substances 0.000 claims description 98
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 80
- 239000012452 mother liquor Substances 0.000 claims description 56
- 239000007853 buffer solution Substances 0.000 claims description 49
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 48
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 45
- 238000001514 detection method Methods 0.000 claims description 37
- UDSAIICHUKSCKT-UHFFFAOYSA-N bromophenol blue Chemical group C1=C(Br)C(O)=C(Br)C=C1C1(C=2C=C(Br)C(O)=C(Br)C=2)C2=CC=CC=C2S(=O)(=O)O1 UDSAIICHUKSCKT-UHFFFAOYSA-N 0.000 claims description 34
- 238000000034 method Methods 0.000 claims description 32
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 claims description 31
- 238000012360 testing method Methods 0.000 claims description 29
- 229920000191 poly(N-vinyl pyrrolidone) Polymers 0.000 claims description 25
- 229920000036 polyvinylpyrrolidone Polymers 0.000 claims description 24
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 claims description 24
- 239000001267 polyvinylpyrrolidone Substances 0.000 claims description 24
- 239000002202 Polyethylene glycol Substances 0.000 claims description 23
- 229920001223 polyethylene glycol Polymers 0.000 claims description 23
- 239000013543 active substance Substances 0.000 claims description 22
- 239000008367 deionised water Substances 0.000 claims description 21
- 229910021641 deionized water Inorganic materials 0.000 claims description 21
- 229960004106 citric acid Drugs 0.000 claims description 18
- WHNWPMSKXPGLAX-UHFFFAOYSA-N N-Vinyl-2-pyrrolidone Chemical compound C=CN1CCCC1=O WHNWPMSKXPGLAX-UHFFFAOYSA-N 0.000 claims description 13
- 229920003081 Povidone K 30 Polymers 0.000 claims description 13
- 229960004543 anhydrous citric acid Drugs 0.000 claims description 13
- FRPHFZCDPYBUAU-UHFFFAOYSA-N Bromocresolgreen Chemical compound CC1=C(Br)C(O)=C(Br)C=C1C1(C=2C(=C(Br)C(O)=C(Br)C=2)C)C2=CC=CC=C2S(=O)(=O)O1 FRPHFZCDPYBUAU-UHFFFAOYSA-N 0.000 claims description 12
- 238000001035 drying Methods 0.000 claims description 12
- 238000002791 soaking Methods 0.000 claims description 10
- 238000004519 manufacturing process Methods 0.000 claims description 6
- 238000002156 mixing Methods 0.000 claims description 6
- -1 quaternary ammonium salt cations Chemical class 0.000 claims description 5
- 239000000872 buffer Substances 0.000 claims description 3
- 239000002904 solvent Substances 0.000 claims description 3
- 239000006179 pH buffering agent Substances 0.000 abstract description 5
- 239000012752 auxiliary agent Substances 0.000 abstract description 3
- 239000000523 sample Substances 0.000 description 71
- LZZYPRNAOMGNLH-UHFFFAOYSA-M Cetrimonium bromide Chemical compound [Br-].CCCCCCCCCCCCCCCC[N+](C)(C)C LZZYPRNAOMGNLH-UHFFFAOYSA-M 0.000 description 15
- 239000011324 bead Substances 0.000 description 14
- 239000012086 standard solution Substances 0.000 description 14
- 238000004448 titration Methods 0.000 description 12
- 239000003153 chemical reaction reagent Substances 0.000 description 10
- 238000004659 sterilization and disinfection Methods 0.000 description 10
- 238000005520 cutting process Methods 0.000 description 9
- 238000005259 measurement Methods 0.000 description 9
- 239000002390 adhesive tape Substances 0.000 description 8
- 239000003086 colorant Substances 0.000 description 7
- 230000000249 desinfective effect Effects 0.000 description 5
- 238000004140 cleaning Methods 0.000 description 4
- 150000002500 ions Chemical class 0.000 description 4
- 239000002253 acid Substances 0.000 description 3
- 238000010923 batch production Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000006174 pH buffer Substances 0.000 description 3
- 230000001954 sterilising effect Effects 0.000 description 3
- 125000002091 cationic group Chemical group 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 238000007598 dipping method Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000009472 formulation Methods 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 231100000252 nontoxic Toxicity 0.000 description 2
- 230000003000 nontoxic effect Effects 0.000 description 2
- 239000007793 ph indicator Substances 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 239000012488 sample solution Substances 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- RBTBFTRPCNLSDE-UHFFFAOYSA-N 3,7-bis(dimethylamino)phenothiazin-5-ium Chemical compound C1=CC(N(C)C)=CC2=[S+]C3=CC(N(C)C)=CC=C3N=C21 RBTBFTRPCNLSDE-UHFFFAOYSA-N 0.000 description 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 1
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- 125000000217 alkyl group Chemical group 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 125000000129 anionic group Chemical group 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 239000003899 bactericide agent Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229960000686 benzalkonium chloride Drugs 0.000 description 1
- 125000001797 benzyl group Chemical group [H]C1=C([H])C([H])=C(C([H])=C1[H])C([H])([H])* 0.000 description 1
- XIWFQDBQMCDYJT-UHFFFAOYSA-M benzyl-dimethyl-tridecylazanium;chloride Chemical compound [Cl-].CCCCCCCCCCCCC[N+](C)(C)CC1=CC=CC=C1 XIWFQDBQMCDYJT-UHFFFAOYSA-M 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 230000003139 buffering effect Effects 0.000 description 1
- 150000001768 cations Chemical class 0.000 description 1
- 229960001701 chloroform Drugs 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000002845 discoloration Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 238000012921 fluorescence analysis Methods 0.000 description 1
- 231100000086 high toxicity Toxicity 0.000 description 1
- 125000004435 hydrogen atom Chemical group [H]* 0.000 description 1
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 1
- 229960000907 methylthioninium chloride Drugs 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000004400 mucous membrane Anatomy 0.000 description 1
- 125000004433 nitrogen atom Chemical group N* 0.000 description 1
- 125000000962 organic group Chemical group 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 239000003206 sterilizing agent Substances 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plasma & Fusion (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The invention discloses a chemical indicator card for detecting concentration of quaternary ammonium salt disinfectant and a preparation method thereof, the chemical indicator card comprises absorbent paper loaded with a color-changing reactive substance and a PVC carrier strip, the absorbent paper is also loaded with an auxiliary agent comprising a leveling agent, a color fixing agent and a pH buffering agent, the color-changing reactive substance, the leveling agent, the color fixing agent and the pH buffering agent are prepared into a mother solution according to a certain preparation sequence, then the absorbent paper is soaked and dried in the mother solution and is attached to the PVC carrier strip, and the chemical indicator card for detecting concentration of quaternary ammonium salt disinfectant is obtained.
Description
Technical Field
The invention relates to the technical field of disinfectant concentration detection, in particular to a chemical indicator card for detecting the concentration of a quaternary ammonium salt disinfectant, a mother solution and a preparation method thereof.
Background
The disinfectant is a preparation which can kill microorganisms on a transmission medium and meet the disinfection requirement. Disinfectants can be classified according to their ability to kill microorganisms: sterilizing agent, high-efficiency disinfectant, middle-efficiency disinfectant and low-efficiency disinfectant.
According to the technical specification WS/T367 of medical institution disinfection, the compound of quaternary ammonium salt disinfectants (such as benzalkonium chloride and the like), alcohols and quaternary ammonium salt compounds belongs to the low-level or medium-level disinfectants. The environmental surface cleaning and disinfecting management standard of the medical institution stipulates the cleaning and management requirements, the cleaning and disinfecting principles and the like of the internal surfaces of buildings of the medical institution and the surfaces of medical equipment and equipment. The standard specifies that the disinfection standards or principles used for disinfecting the surfaces of general objects of medical institutions and the surfaces of medical instruments are different, and the corresponding disinfectant concentrations are different, for example, the quaternary ammonium salt disinfectant with the concentration of 500mg/L can be used for disinfecting the surfaces of the general objects of the medical institutions, and the quaternary ammonium salt disinfectant with the concentration of 1000mg/L can be used for disinfecting the surfaces of the medical instruments, fabrics, surgical hands, sanitary hands, skins and mucous membranes. Therefore, it is important to identify the concentration of disinfectant formulation to ensure that the concentration of disinfectant formulation meets the disinfection requirements.
At present, methods for detecting the concentration of the quaternary ammonium salt comprise a two-phase titration method, a spectrophotometry method, a fluorescence analysis method and the like, but the methods are not suitable for the operation of common cleaning and disinfection personnel in medical institution scenes because large and professional analytical instruments and equipment or reagents with high toxicity are required. In the technical specification of disinfection, the concentration of quaternary ammonium salt is measured in an acid environment by taking an anionic dye bromophenol blue as an indicator of a two-phase titration method, quantitative ion association reaction can be carried out between the bromophenol blue and quaternary ammonium salt cations to generate a blue ion association complex to indicate a titration end point, and a trichloromethane organic solvent is required to be used as an extracting agent in the method, so that the method has toxicity and can cause harm to human bodies and the environment.
The invention utilizes the cationic property of quaternary ammonium salt disinfectant. The cation of quaternary ammonium salt is composed of ammonium cation [ H ]4N+]The four hydrogen atoms are replaced by methyl, saturated or unsaturated long-chain alkyl, benzyl and other organic groups, and the four groups and the N atom form a cationic group which is an effective part of the bactericide.
The bromophenol blue can be used as a pH indicator, the pH indicator develops acid color-yellow when the pH is 3, the bromophenol blue and quaternary ammonium salt cations generate ion association reaction, the generated ion association complex is blue, the acid color yellow disappears, and the apparent color is a composite color of yellow and blue, namely green-blue.
For example, in the prior art, chinese patent publication No. CN104198480A, "test paper for detecting quaternary ammonium salt in water quality and method for preparing the same", the prepared test paper utilizes the color change of bromocresol green and methylene blue solution to indicate the change of the concentration of quaternary ammonium salt in water, but the test paper has many kinds of raw material reagents, complicated preparation steps, complex process and correspondingly high cost. The invention utilizes the color change principle of bromophenol blue to prepare the chemical indicator card for detecting the concentration of the quaternary ammonium salt disinfectant, can detect different concentrations in a wider range, is portable to use and has high detection sensitivity.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention provides the mother liquor for manufacturing the chemical indicator card for detecting the concentration of the quaternary ammonium salt disinfectant, and the mother liquor is friendly to human body and environment, safe, nontoxic, simple in reagent type, low in cost and suitable for factory batch production.
In order to achieve the purpose, the invention adopts the technical scheme that: a mother liquor for manufacturing a quaternary ammonium salt disinfectant concentration detection chemical indicator card is characterized in that: the mother liquor comprises a pH buffer solution, a leveling agent, a color fixing agent and a color-changing reactive substance capable of reacting with quaternary ammonium salt cations, wherein the pH of the mother liquor is 2.8-3.8, the buffer pair substances of the pH buffer solution are citric acid and disodium hydrogen phosphate, the color-changing reactive substance is bromophenol blue or bromocresol green, the mass fraction of the color-changing reactive substance in the mother liquor is 0.03-0.1%, the volume fraction of a solvent is 95% ethanol, the mass fraction of 95% ethanol in the mother liquor is 19-20%, the mass fraction of the leveling agent in the mother liquor is 0.1-1%, and the mass fraction of the color fixing agent in the mother liquor is 3.2%.
Further, the color-changing reactive substance comprises bromophenol blue or bromocresol green, when the bromophenol blue is selected as the color-changing reactive substance, the pH of the mother liquor is adjusted to be 2.8-3.0 through a pH buffer solution, and when the bromocresol green is selected as the color-changing reactive substance, the pH of the mother liquor is adjusted to be 3.6-3.8 through a pH buffer solution.
Further, the leveling agent is peregal O-25 or peregal O-20.
Further, the color fixing agent is polyethylene glycol and polyvinylpyrrolidone (PVP K30), and the content of the color fixing agent in the mother liquor is 1.2% and 2%, respectively.
By adopting the scheme, the reagent used in the mother liquor formula is non-toxic, the preparation process is simple, and the batch production is convenient.
Another object of the invention is: the invention also provides a preparation method of the chemical indicator card for detecting the concentration of the quaternary ammonium salt disinfectant, which is characterized by comprising the following preparation steps:
s1: dissolving a color-changing active substance and a leveling agent by using 95% ethanol in volume fraction to obtain a solution A, wherein the mass fractions of the color-changing active substance and the leveling agent are respectively 0.03-0.1% and 0.1-1% of a mother solution, and the mass fraction of 95% ethanol in the mother solution is 19-20%;
s2: preparing a pH buffer solution: preparing pH buffer solutions from anhydrous citric acid and disodium hydrogen phosphate by using deionized water respectively;
s3: sequentially dissolving polyethylene glycol and polyvinylpyrrolidone (PVP K30) in a pH buffer solution with a formula amount to ensure that the mass fractions of the polyethylene glycol and the polyvinylpyrrolidone in the mother solution are 1.2% and 2% respectively to obtain a solution B;
s4: uniformly mixing the solution A and the solution B to obtain mother liquor, wherein the pH of the mother liquor is 2.8-3.8;
s5: soaking absorbent paper in the mother liquor for 10-15min, and drying at 55-70 ℃ to obtain absorbent paper containing the color-changing reactive active substances;
s6: and (5) fixing the absorbent paper of S5 on a carrier card to obtain the quaternary ammonium salt disinfectant concentration indication card.
Further, when the color-changing active substance is bromophenol blue, the pH of the mother liquor is adjusted to 2.8-3.0 by a pH buffer solution.
Further, the anhydrous citric acid and the disodium hydrogen phosphate are respectively prepared into 0.1mol/L and 0.2mol/L solutions by deionized water; 0.2mol/L disodium hydrogen phosphate solution and 0.1mol/L citric acid solution are mixed according to the volume ratio of 2.18:17.82 to prepare pH buffer solution with the pH value of 2.6.
Further, when the color-changing reaction active substance is bromocresol green, the pH of the mother liquor is adjusted to 3.6-3.8 by a pH buffer solution.
Further, the anhydrous citric acid and the disodium hydrogen phosphate are respectively prepared into 0.1mol/L and 0.2mol/L solutions by deionized water; 0.2mol/L disodium hydrogen phosphate solution and 0.1mol/L citric acid solution are mixed according to the volume ratio of 6.44:13.56 to prepare pH buffer solution with the pH value of 3.6.
By adopting the scheme, the preparation method disclosed by the invention has the following beneficial effects:
the method has the advantages of safe and nonhazardous reagents, few types of reagents, simple process, low cost and relatively short time consumption of the whole process, and is suitable for batch production in factories. In the preparation process, the pH buffer solution is prepared firstly, and then other raw materials are dissolved, so that the pH buffer solution and other substances are prevented from reacting in advance, and the buffering effect is reduced.
Another object of the invention is: the invention also provides the quaternary ammonium salt disinfectant concentration detection test paper or the quaternary ammonium salt disinfectant concentration detection chemical indicator card obtained by the preparation method of the quaternary ammonium salt disinfectant concentration detection chemical indicator card.
By adopting the scheme, the chemical indicator card for detecting the concentration of the quaternary ammonium salt disinfectant comprises absorbent paper loaded with a color-changing reactive substance and a PVC carrier strip, wherein the absorbent paper is also loaded with an auxiliary agent, and the auxiliary agent comprises a leveling agent, a color fixing agent and a pH buffering agent. According to a certain preparation sequence, preparing a color-changing reaction active substance, a leveling agent, a color fixing agent and a pH buffering agent into a mother solution, then dipping water-absorbing paper in the mother solution, drying the water-absorbing paper, and then sticking the water-absorbing paper to a PVC carrier strip to obtain the chemical indicator card for detecting the concentration of the quaternary ammonium salt disinfectant.
The indicator card has the following advantages: the concentration of the quaternary ammonium salt sterilization factor in the quaternary ammonium salt disinfectant can be rapidly detected, and the method is suitable for detecting the sterilization factor during the sterilization of the quaternary ammonium salt on the surface of a hospital object; the quaternary ammonium salt disinfectant can detect different concentrations in a wide range, and the detection range is 0-1000 ppm. The indicating card also has the advantages of portability, high detection sensitivity, high detection speed and the like. In addition, the color-changing reactive substance on the chemical indicator card can be uniformly and firmly loaded on the absorbent paper, so that the color development is stable and uniform, and the problem that the color-changing reactive substance falls off and enters a solution to be detected to cause color development failure during detection is avoided.
The invention is further described below with reference to the accompanying drawings.
Drawings
FIG. 1 is a chart of a color chart according to embodiment 1 of the present invention;
FIG. 2 is a chart of a color chart according to embodiment 2 of the present invention;
FIG. 3 is a chart of a color chart according to embodiment 3 of the present invention;
FIG. 4 is a chart of a color chart according to embodiment 4 of the present invention;
FIG. 5 is a chart of a color chart according to embodiment 5 of the present invention;
FIG. 6 is a chart of a color chart according to embodiment 6 of the present invention;
FIG. 7 is a chart of a color chart according to embodiment 7 of the present invention.
Detailed Description
The chemical indicator card for detecting the concentration of the quaternary ammonium salt disinfectant in the specific embodiment of the invention is prepared by the following method: firstly, preparing a mother solution from a color-changing reaction active substance bromophenol blue (or bromocresol green), a solvent, a pH buffering agent, a leveling agent and a color fixing agent, then dipping absorbent paper in the mother solution, drying to obtain absorbent paper capable of changing color, and cutting the absorbent paper and fixing the absorbent paper on a PVC carrier strip. Wherein, the mass fraction of the discoloration reaction active substance in the mother solution is 0.03-0.1%, and the pH buffer takes citric acid and disodium hydrogen phosphate as a buffer pair; the leveling agent is peregal O-25 or O-20, and the mass fraction of the leveling agent in the mother liquor is 0-1%; the color fixing agents are polyethylene glycol and polyvinylpyrrolidone (PVP K30), and the content of the two color fixing agents in the mother liquor is 1.2% and 2% respectively;
the method comprises the following specific steps:
(1) dissolving bromophenol blue/bromocresol green with 95% volume fraction ethanol, and adding peacefully to obtain solution A; the peregal can be peregal O-25 and peregal O-20, and the peregal O-25 and bromophenol blue are used for illustration in the embodiment, and the bromophenol blue can be developed when the pH of the mother liquor is 2.8-3.0, but the pH of the mother liquor is 2.8 for illustration in the embodiment;
(2) preparing a pH buffer solution: preparing citric acid and disodium hydrogen phosphate into 0.1mol/L citric acid solution and 0.2mol/L disodium hydrogen phosphate solution respectively by using deionized water; when 0.2mol/L disodium hydrogen phosphate solution and 0.1mol/L citric acid solution are mixed according to the volume ratio of 2.18:17.82 to prepare a pH buffer solution with the pH of 2.6, and the color-changing reaction active substance is bromocresol green, the preparation method of the pH buffer solution is as follows: 0.2mol/L disodium hydrogen phosphate solution and 0.1mol/L citric acid solution are mixed according to the volume ratio of 6.44:13.56 to prepare pH buffer solution with the pH of 3.6, and the pH buffer solution is weighed according to the formula amount when in use.
(3) Sequentially dissolving polyethylene glycol and polyvinylpyrrolidone (PVP K30) in a pH buffer solution with a formula amount to obtain a solution B;
(4) uniformly mixing the solution A and the solution B to obtain mother liquor, wherein the pH of the mother liquor is 2.8-3.0; wherein, in the step (1), the mass fractions of bromophenol blue and peregal are respectively 0.03-0.05% and 0.05-0.3% of the mother liquor, and the mass fraction of 95% ethanol in the mother liquor is 20%; in the step (3), the mass fractions of the polyethylene glycol and the polyvinyl pyrrolidone in the mother liquor are respectively 1.2% and 2%;
(5) soaking absorbent paper in the mother liquor for 10-15min, and drying at 55-70 ℃ to obtain absorbent paper containing the color-changing reactive active substances; the absorbent paper is absorbent fiber, the thickness is 0.35mm, and the gram weight is 115g/m 2;
(6) and (3) cutting the water absorbing paper in the step (5) into 5 multiplied by 5mm, fixing one end of a PVC strip carrier card with the width of 5mm, the length of 90mm and the thickness of 0.3mm by using a double-sided adhesive tape, and obtaining the chemical indicator card for detecting the concentration of the quaternary ammonium salt disinfectant.
The detection method of the concentration detection chemical indicator card comprises the following steps: (1) immersing a chemical indicator card for detecting the concentration of the quaternary ammonium salt disinfectant into the disinfectant sample solution to be detected for 3 seconds, and then taking out; (2) and throwing off the redundant solution on the indicator card, reacting for 15 seconds, and comparing with a standard colorimetric card to obtain the quaternary ammonium salt concentration value of the disinfectant sample to be detected.
Example 1: (variables are 0.03 mass percent of bromophenol blue, 0.1 mass percent of peregal O-25, 19.4 mass percent of ethanol, 1.2 mass percent of polyethylene glycol and 2 mass percent of polyvinylpyrrolidone)
A solution A was prepared by dissolving 0.025g bromophenol blue and 0.0825g peregal O-25 in 16.67g volume fraction 95% ethanol.
pH buffer solution: preparing 0.1mol/L of anhydrous citric acid solution by using deionized water, preparing 0.2mol/L of disodium hydrogen phosphate solution by using the deionized water, and preparing a pH buffer solution according to the volume ratio of the disodium hydrogen phosphate to the citric acid of 2.18:17.82, wherein the pH is 2.6.
0.99g of polyethylene glycol and 1.65g of polyvinylpyrrolidone (PVP K30) were dissolved in sequence in 66.6g of the above pH buffer and mixed well to obtain solution B.
The solution A and the solution B were mixed uniformly to obtain 86.02g of mother liquor, the pH of which was 2.8.
Soaking the absorbent paper in the mother liquor for 10-15min, and drying in an oven at 55-70 ℃ to obtain the absorbent paper containing the color-changing reactive active substance.
Cutting the dried absorbent paper into 5 multiplied by 5mm, fixing one end of a PVC strip carrier card with the width of 5mm, the length of 90mm and the thickness of 0.3mm by using a double-sided adhesive tape, and obtaining the chemical indicator card for detecting the concentration of the quaternary ammonium salt disinfectant.
The chemical indicator card for detecting the concentration is used for semi-quantitatively detecting the content of the quaternary ammonium salt in a quaternary ammonium salt disinfectant sample, the detection time is shortened, the detection of the quaternary ammonium salt disinfectant prepared in a hospital can be completed within 15 seconds, and the measurement range of the quaternary ammonium salt is 0-1000 mg/L.
The chemical indicator card of the embodiment is adopted to detect the concentration of the quaternary ammonium salt in the quaternary ammonium salt disinfectant solution sample to be detected, and the detection method comprises the following steps:
(1) immersing the end of the chemical indicator card into a sample to be detected for 3 seconds, taking out the end and throwing away redundant water beads; and (2) waiting for the indicator card color block to react with the solution for 15 seconds, and immediately comparing the indicator card color block with a standard color comparison card to obtain a measured value.
The determination method of the color card type (Pantong color number) on the color card comprises the following steps:
preparing a hexadecyl trimethyl ammonium bromide standard solution with the concentration of 250mg/L by using pure water, immersing the indicator card prepared in the example 1 into the solution, taking out the indicator card after 3 seconds, throwing away redundant water beads, and finding out a standard color with the consistent color on a pantone color card according to the color presented by the test paper after 15 seconds; respectively measuring the standard colors of the indicator card when the concentrations of the hexadecyl trimethyl ammonium bromide standard solution are 0mg/L, 500mg/L and 1000mg/L by the same method; comparing with Pantong color card to obtain color value of each standard color, and performing color matching and printing with computer to obtain standard color comparison card 1 #.
The corresponding relationship between the 1# colorimetric card and the concentration of the quaternary ammonium salt is as follows:
PANTONE 100C-0mg/L；
PANTONE 374C-250mg/L；
PANTONE 377C-500mg/L；
PANTONE 342U-1000mg/L。
example 2 (variables: bromophenol blue mass fraction 0.04%, peregal O-25 mass fraction 0.1%, ethanol mass fraction 19.4%, polyethylene glycol mass fraction 1.2%, and polyvinyl pyrrolidone mass fraction 2%) 12.5g of 95% volume fraction ethanol was dissolved in 0.025g of bromophenol blue and 0.0625g of peregal O-25 to obtain solution A.
pH buffer solution: preparing 0.1mol/L of anhydrous citric acid solution by using deionized water, preparing 0.2mol/L of disodium hydrogen phosphate solution by using the deionized water, and preparing a pH buffer solution according to the volume ratio of the disodium hydrogen phosphate to the citric acid of 2.18:17.82, wherein the pH is 2.6.
0.75g of polyethylene glycol and 1.25g of polyvinylpyrrolidone (PVP K30) were dissolved in the above pH buffer solution in that order and mixed to obtain solution B.
The solution A and the solution B were mixed uniformly to obtain 64.59g of mother liquor, the pH of which was 2.8.
Soaking the absorbent paper in the mother liquor for 10-15min, and drying in an oven at 55-70 ℃ to obtain the absorbent paper containing the color-changing reactive active substance.
Cutting the dried absorbent paper into 5 multiplied by 5mm, fixing one end of a PVC strip carrier card with the width of 5mm, the length of 90mm and the thickness of 0.3mm by using a double-sided adhesive tape, and obtaining the chemical indicator card for detecting the concentration of the quaternary ammonium salt disinfectant.
The chemical indicator card for detecting the concentration is used for semi-quantitatively detecting the content of the quaternary ammonium salt in a quaternary ammonium salt disinfectant sample, the detection time is shortened, the detection of the quaternary ammonium salt disinfectant prepared in a hospital can be completed within 15 seconds, and the measurement range of the quaternary ammonium salt is 0-1000 mg/L.
The chemical indicator card of the embodiment is adopted to detect the concentration of the quaternary ammonium salt in the quaternary ammonium salt disinfectant solution sample to be detected, and the detection method comprises the following steps:
(1) immersing the end of the chemical indicator card into a sample to be detected for 3 seconds, taking out the end and throwing away redundant water beads; and (2) waiting for the indicator card color block to react with the solution for 15 seconds, and immediately comparing the indicator card color block with a standard color comparison card to obtain a measured value.
The determination method of the color card type (Pantong color number) on the color card comprises the following steps:
preparing a hexadecyl trimethyl ammonium bromide standard solution with the concentration of 250mg/L by using pure water, immersing the indicator card prepared in the example 2 into the solution, taking out the indicator card after 3 seconds, throwing away redundant water beads, and finding out a standard color with the consistent color on a pantone color card according to the color presented by the test paper after 15 seconds; respectively measuring the standard colors of the indicator card when the concentrations of the hexadecyl trimethyl ammonium bromide standard solution are 0mg/L, 500mg/L and 1000mg/L by the same method; comparing with Pantong color card to obtain color value of each standard color, and performing color matching and printing with computer to obtain standard color comparison card 2 #.
The corresponding relationship between the 2# colorimetric card and the concentration of the quaternary ammonium salt is as follows:
PANTONE 142C-0mg/L；
PANTONE 368C-250mg/L；
PANTONE 329C-500mg/L；
PANTONE 3308U-1000mg/L。
example 3 (variables: bromophenol blue mass fraction 0.05%, peregal O-25 mass fraction 0.1%, ethanol mass fraction 19.3%, polyethylene glycol mass fraction 1.2%, and polyvinyl pyrrolidone mass fraction 2%) 0.025g of bromophenol blue and 0.05g of peregal O-25 were dissolved in 10g of 95% volume fraction ethanol to obtain solution A.
pH buffer solution: preparing 0.1mol/L of anhydrous citric acid solution by using deionized water, preparing 0.2mol/L of disodium hydrogen phosphate solution by using the deionized water, and preparing a pH buffer solution according to the volume ratio of the disodium hydrogen phosphate to the citric acid of 2.18:17.82, wherein the pH is 2.6.
0.6g of polyethylene glycol and 1.0g of polyvinylpyrrolidone (PVP K30) were dissolved in 40g of the above pH buffer solution in this order and mixed well to obtain solution B.
And uniformly mixing the solution A and the solution B to obtain a mother solution 51.68gl, wherein the pH value of the mother solution is 2.8.
Soaking the absorbent paper in the mother liquor for 10-15min, and drying in an oven at 55-70 ℃ to obtain the absorbent paper containing the color-changing reactive active substance.
Cutting the dried absorbent paper into 5 multiplied by 5mm, fixing one end of a PVC strip carrier card with the width of 5mm, the length of 90mm and the thickness of 0.3mm by using a double-sided adhesive tape, and obtaining the chemical indicator card for detecting the concentration of the quaternary ammonium salt disinfectant.
The chemical indicator card for detecting the concentration is used for semi-quantitatively detecting the content of the quaternary ammonium salt in a quaternary ammonium salt disinfectant sample, the detection time is shortened, the detection of the quaternary ammonium salt disinfectant prepared in a hospital can be completed within 15 seconds, and the measurement range of the quaternary ammonium salt is 0-1000 mg/L.
The chemical indicator card of the embodiment is adopted to detect the concentration of the quaternary ammonium salt in the quaternary ammonium salt disinfectant solution sample to be detected, and the detection method comprises the following steps:
(1) immersing the end of the chemical indicator card into a sample to be detected for 3 seconds, taking out the end and throwing away redundant water beads; and (2) waiting for the indicator card color block to react with the solution for 15 seconds, and immediately comparing the indicator card color block with a standard color comparison card to obtain a measured value.
The determination method of the color card type (Pantong color number) on the color card comprises the following steps:
preparing a hexadecyl trimethyl ammonium bromide standard solution with the concentration of 250mg/L by using pure water, immersing the indicator card prepared in the embodiment 3 into the solution, taking out the indicator card after 3 seconds, throwing away redundant water beads, and finding out a standard color with the consistent color on a pantone color card according to the color presented by the test paper after 15 seconds; respectively measuring the standard colors of the indicator card when the concentrations of the hexadecyl trimethyl ammonium bromide standard solution are 0mg/L, 500mg/L and 1000mg/L by the same method; comparing with Pantong color card to obtain color value of each standard color, and performing color matching and printing with computer to obtain standard color comparison card 3 #.
The corresponding relationship between the 3# colorimetric card and the concentration of the quaternary ammonium salt is as follows:
PANTONE 100C-0mg/L；
PANTONE 368C-250mg/L；
PANTONE 3415C-500mg/L；
PANTONE 3292U-1000mg/L。
example 4 (variables: bromophenol blue mass fraction 0.1%, peregal O-25 mass fraction 0.1%, ethanol mass fraction 19.3%, polyethylene glycol mass fraction 1.2%, polyvinyl pyrrolidone mass fraction 2%)
A solution A was prepared by dissolving 0.025g of bromophenol blue and 0.025g of peregal O-25 in 5g of 95% ethanol by volume.
pH buffer solution: preparing 0.1mol/L of anhydrous citric acid solution by using deionized water, preparing 0.2mol/L of disodium hydrogen phosphate solution by using the deionized water, and preparing a pH buffer solution according to the volume ratio of the disodium hydrogen phosphate to the citric acid of 2.18:17.82, wherein the pH is 2.6.
0.3g of polyethylene glycol and 0.5g of polyvinylpyrrolidone (PVP K30) were dissolved in the above pH buffer solution in this order and mixed to obtain solution B.
And uniformly mixing the solution A and the solution B to obtain 25.85 of mother liquor, wherein the pH value of the mother liquor is 2.9.
Soaking the absorbent paper in the mother liquor for 10-15min, and drying in an oven at 55-70 ℃ to obtain the absorbent paper containing the color-changing reactive active substance.
Cutting the dried absorbent paper into 5 multiplied by 5mm, fixing one end of a PVC strip carrier card with the width of 5mm, the length of 90mm and the thickness of 0.3mm by using a double-sided adhesive tape, and obtaining the chemical indicator card for detecting the concentration of the quaternary ammonium salt disinfectant.
The chemical indicator card for detecting the concentration is used for semi-quantitatively detecting the content of the quaternary ammonium salt in a quaternary ammonium salt disinfectant sample, the detection time is shortened, the detection of the quaternary ammonium salt disinfectant prepared in a hospital can be completed within 15 seconds, and the measurement range of the quaternary ammonium salt is 0-1000 mg/L.
The chemical indicator card of the embodiment is adopted to detect the concentration of the quaternary ammonium salt in the quaternary ammonium salt disinfectant solution sample to be detected, and the detection method comprises the following steps:
(1) immersing the end of the chemical indicator card into a sample to be detected for 3 seconds, taking out the end and throwing away redundant water beads; and (2) waiting for the indicator card color block to react with the solution for 15 seconds, and immediately comparing the indicator card color block with a standard color comparison card to obtain a measured value.
The determination method of the color card type (Pantong color number) on the color card comprises the following steps:
preparing a hexadecyl trimethyl ammonium bromide standard solution with the concentration of 250mg/L by using pure water, immersing the indicator card prepared in the embodiment 4 into the solution, taking out the indicator card after 3 seconds, throwing away redundant water beads, and finding out a standard color with the consistent color on a pantone color card according to the color presented by the test paper after 15 seconds; respectively measuring the standard colors of the indicator card when the concentrations of the hexadecyl trimethyl ammonium bromide standard solution are 0mg/L, 500mg/L and 1000mg/L by the same method; comparing with Pantong color card to obtain color value of each standard color, and performing color matching and printing with computer to obtain standard color comparison card No. 4.
The corresponding relationship between the 4# colorimetric card and the concentration of the quaternary ammonium salt is as follows:
PANTONE 367C-0mg/L；
PANTONE 383C-250mg/L；
PANTONE 349C-500mg/L；
PANTONE 3308C-1000mg/L。
the reagents used in examples 1 to 4 were different in the mass fraction of bromophenol blue (0.03%, 0.04%, 0.05%, 0.1%, respectively). As shown by the following test paper effect test, although the mass fraction of bromophenol blue in the above examples is different, the test paper prepared in each of the four examples can develop color and have distinction when tested in 250,500,1000ppm (see color chart, FIG. 1-FIG. 4).
Example 5 (variables: bromophenol blue mass fraction 0.1%, peregal O-25 mass fraction 0.5%, ethanol mass fraction 19.3%, polyethylene glycol mass fraction 1.2%, polyvinyl pyrrolidone mass fraction 2%)
A solution A was prepared by dissolving 0.025g of bromophenol blue and 0.125g of peregal O-25 in 5g of 95% ethanol by volume.
pH buffer solution: preparing 0.1mol/L of anhydrous citric acid solution by using deionized water, preparing 0.2mol/L of disodium hydrogen phosphate solution by using the deionized water, and preparing a pH buffer solution according to the volume ratio of the disodium hydrogen phosphate to the citric acid of 2.18:17.82, wherein the pH is 2.6.
0.3g of polyethylene glycol and 0.5g of polyvinylpyrrolidone (PVP K30) were dissolved in the above pH buffer solution in this order and mixed to obtain solution B.
And uniformly mixing the solution A and the solution B to obtain 25.95g of mother liquor, wherein the pH value of the mother liquor is 2.8.
Soaking the absorbent paper in the mother liquor for 10-15min, and drying in an oven at 55-70 ℃ to obtain the absorbent paper containing the color-changing reactive active substance.
Cutting the dried absorbent paper into 5 multiplied by 5mm, fixing one end of a PVC strip carrier card with the width of 5mm, the length of 90mm and the thickness of 0.3mm by using a double-sided adhesive tape, and obtaining the chemical indicator card for detecting the concentration of the quaternary ammonium salt disinfectant.
The chemical indicator card for detecting the concentration is used for semi-quantitatively detecting the content of the quaternary ammonium salt in a quaternary ammonium salt disinfectant sample, the detection time is shortened, the detection of the quaternary ammonium salt disinfectant prepared in a hospital can be completed within 15 seconds, and the measurement range of the quaternary ammonium salt is 0-1000 mg/L.
The chemical indicator card of the embodiment is adopted to detect the concentration of the quaternary ammonium salt in the quaternary ammonium salt disinfectant solution sample to be detected, and the detection method comprises the following steps:
(1) immersing the end of the chemical indicator card into a sample to be detected for 3 seconds, taking out the end and throwing away redundant water beads; and (2) waiting for the indicator card color block to react with the solution for 15 seconds, and immediately comparing the indicator card color block with a standard color comparison card to obtain a measured value.
The determination method of the color card type (Pantong color number) on the color card comprises the following steps:
preparing a hexadecyl trimethyl ammonium bromide standard solution with the concentration of 250mg/L by using pure water, immersing the indicator card prepared in the example 5 into the solution, taking out the indicator card after 3 seconds, throwing away redundant water beads, and finding out a standard color with the consistent color on a pantone color card according to the color presented by the test paper after 15 seconds; respectively measuring the standard colors of the indicator card when the concentrations of the hexadecyl trimethyl ammonium bromide standard solution are 0mg/L, 500mg/L and 1000mg/L by the same method; comparing with Pantong color card to obtain color value of each standard color, and performing color matching and printing with computer to obtain standard color comparison card No. 5.
The corresponding relationship between the 5# colorimetric card and the concentration of the quaternary ammonium salt is as follows:
PANTONE 381C-0mg/L；
PANTONE 368C-250mg/L；
PANTONE 378C-500mg/L；
PANTONE 3435C-1000mg/L。
example 6 (variables: bromophenol blue mass fraction 0.1%, peregal O-25 mass fraction 1%, ethanol mass fraction 19.2%, polyethylene glycol mass fraction 1.2%, polyvinyl pyrrolidone mass fraction 2%)
A solution A was prepared by dissolving 0.025g of bromophenol blue and 0.25g of peregal O-25 in 5g of 95% ethanol by volume.
pH buffer solution: preparing 0.1mol/L of anhydrous citric acid solution by using deionized water, preparing 0.2mol/L of disodium hydrogen phosphate solution by using the deionized water, and preparing a pH buffer solution according to the volume ratio of the disodium hydrogen phosphate to the citric acid of 2.18:17.82, wherein the pH is 2.6.
0.3g of polyethylene glycol and 0.5g of polyvinylpyrrolidone (PVP K30) were dissolved in the above pH buffer solution in this order and mixed to obtain solution B.
The solution A and the solution B are mixed evenly to obtain 26.08g of mother liquor, and the pH value of the mother liquor is 2.8.
Soaking the absorbent paper in the mother liquor for 10-15min, and drying in an oven at 55-70 ℃ to obtain the absorbent paper containing the color-changing reactive active substance.
Cutting the dried absorbent paper into 5 multiplied by 5mm, fixing one end of a PVC strip carrier card with the width of 5mm, the length of 90mm and the thickness of 0.3mm by using a double-sided adhesive tape, and obtaining the chemical indicator card for detecting the concentration of the quaternary ammonium salt disinfectant.
The chemical indicator card for detecting the concentration is used for semi-quantitatively detecting the content of the quaternary ammonium salt in a quaternary ammonium salt disinfectant sample, the detection time is shortened, the detection of the quaternary ammonium salt disinfectant prepared in a hospital can be completed within 15 seconds, and the measurement range of the quaternary ammonium salt is 0-1000 mg/L.
The chemical indicator card of the embodiment is adopted to detect the concentration of the quaternary ammonium salt in the quaternary ammonium salt disinfectant solution sample to be detected, and the detection method comprises the following steps:
(1) immersing the end of the chemical indicator card into a sample to be detected for 3 seconds, taking out the end and throwing away redundant water beads; and (2) waiting for the indicator card color block to react with the solution for 15 seconds, and immediately comparing the indicator card color block with a standard color comparison card to obtain a measured value.
The determination method of the color card type (Pantong color number) on the color card comprises the following steps:
preparing a hexadecyl trimethyl ammonium bromide standard solution with the concentration of 250mg/L by using pure water, immersing the indicator card prepared in the example 6 into the solution, taking out the indicator card after 3 seconds, throwing away redundant water beads, and finding out a standard color with the consistent color on a pantone color card according to the color presented by the test paper after 15 seconds; respectively measuring the standard colors of the indicator card when the concentrations of the hexadecyl trimethyl ammonium bromide standard solution are 0mg/L, 500mg/L and 1000mg/L by the same method; comparing with Pantong color card to obtain color value of each standard color, and performing color matching and printing with computer to obtain standard color comparison card 6 #.
The corresponding relationship between the 6# colorimetric card and the concentration of the quaternary ammonium salt is as follows:
PANTONE 381C-0mg/L；
PANTONE 383C-250mg/L；
PANTONE 347C-500mg/L；
PANTONE 330C-1000mg/L。
the reagents used in examples 4 to 5 to 6 were varied in the concentration of the peregal (0.1%, 0.5%, 1%, respectively), and the water-absorbent paper area was uniformly colored when tested using the three test strips.
Control-example 7 (variables: bromophenol blue mass fraction 0.03%, peregal O-25 mass fraction 0.0%, ethanol mass fraction 19.4%, polyethylene glycol mass fraction 1.2%, and polyvinyl pyrrolidone mass fraction 2%) solution A was prepared by dissolving 0.025g bromophenol blue in 16.67g of 95% volume fraction ethanol.
pH buffer solution: preparing 0.1mol/L of anhydrous citric acid solution by using deionized water, preparing 0.2mol/L of disodium hydrogen phosphate solution by using the deionized water, and preparing a pH buffer solution according to the volume ratio of the disodium hydrogen phosphate to the citric acid of 2.18:17.82, wherein the pH is 2.6.
0.99g of polyethylene glycol and 1.65g of polyvinylpyrrolidone (PVP K30) were dissolved in sequence in 66.6g of the above pH buffer and mixed well to obtain solution B.
The solution A and the solution B are mixed evenly to obtain 85.94g of mother liquor, and the pH value of the mother liquor is 2.8.
Soaking the absorbent paper in the mother liquor for 10-15min, and drying in an oven at 55-70 ℃ to obtain the absorbent paper containing the color-changing reactive active substance.
Cutting the dried absorbent paper into 5 multiplied by 5mm, fixing one end of a PVC strip carrier card with the width of 5mm, the length of 90mm and the thickness of 0.3mm by using a double-sided adhesive tape, and obtaining the chemical indicator card for detecting the concentration of the quaternary ammonium salt disinfectant.
The chemical indicator card for detecting the concentration is used for semi-quantitatively detecting the content of the quaternary ammonium salt in a quaternary ammonium salt disinfectant sample, the detection time is shortened, the detection of the quaternary ammonium salt disinfectant prepared in a hospital can be completed within 15 seconds, and the measurement range of the quaternary ammonium salt is 0-1000 mg/L.
The chemical indicator card of the embodiment is adopted to detect the concentration of the quaternary ammonium salt in the quaternary ammonium salt disinfectant solution sample to be detected, and the detection method comprises the following steps:
(1) immersing the end of the chemical indicator card into a sample to be detected for 3 seconds, taking out the end and throwing away redundant water beads; and (2) waiting for the indicator card color block to react with the solution for 15 seconds, and immediately comparing the indicator card color block with a standard color comparison card to obtain a measured value.
The determination method of the color card type (Pantong color number) on the color card comprises the following steps:
preparing a hexadecyl trimethyl ammonium bromide standard solution with the concentration of 250mg/L by using pure water, immersing the indicator card prepared in example 7 into the solution, taking out the indicator card after 3 seconds, throwing away redundant water beads, and finding out a standard color with the consistent color on a pantone color card according to the color presented by the test paper after 15 seconds; respectively measuring the standard colors of the indicator card when the concentrations of the hexadecyl trimethyl ammonium bromide standard solution are 0mg/L, 500mg/L and 1000mg/L by the same method; comparing with Pantong color card to obtain color value of each standard color, and performing color matching and printing with computer to obtain standard color comparison card 7 #.
The corresponding relationship between the 7# colorimetric card and the concentration of the quaternary ammonium salt is as follows:
PANTONE 100C-0mg/L；
PANTONE 374C-250mg/L；
PANTONE 377C-500mg/L；
PANTONE 342U-1000mg/L。
the comparison of examples 1 and 7 shows that the reagent used was the reagent with a variable of the presence or absence of the addition of the peregal (0.1% and/0, respectively), and the test paper of examples 1 and 7 showed that the color of the absorbent paper area of example 1 was uniform, whereas the color of the absorbent paper area of example 7 was different and non-uniform, but the detection accuracy was not affected by the following table 7.
Test effect comparison experiment of quaternary ammonium salt concentration:
the chemical indicator card 1# -7# for detecting the concentration of the quaternary ammonium salt disinfectant in the embodiment 1-7 of the invention is selected for carrying out a comparative experiment of the test effect of the concentration of the quaternary ammonium salt. The test method comprises the following steps: a series of sample solutions of cetyltrimethylammonium bromide with different concentrations were prepared and the concentrations were determined using the titration method in appendix A of the Disinfection Specification (2002 edition). When the color tone of the sample color developing solution is consistent with the standard color level, the color level indicating concentration is directly taken as a measured value, and if the color is inconsistent, two adjacent standard color levels are selected according to the color tone of the sample color developing solution to obtain a sample concentration range. The test results are shown in tables 1-6.
Table 1 example 1 test results vs experimental results:
| sample number | Titration method (mg/L) | Indicator card of example 1 (mg/L) |
| Sample 1 | 0 | 0 |
| Sample 3 | 248.5 | 250 |
| Sample No. 5 | 504.3 | 500 |
| Sample 7 | 997.6 | 1000 |
Table 2 example 2 test results vs experimental results:
| sample number | Titration method (mg/L) | Indicator card of example 2 (mg/L) |
| Sample 1 | 0 | 0 |
| Sample 2 | 198.4 | >0,<250 |
| Sample 3 | 248.5 | 250 |
| Sample No. 4 | 378.1 | >250,<500 |
| Sample No. 5 | 504.3 | 500 |
| Sample No. 6 | 753.2 | >500,<1000 |
| Sample 7 | 997.6 | 1000 |
Table 3 example 3 test results vs experimental results:
| sample number | Titration method (mg/L) | Indicator card of example 3 (mg/L) |
| Sample 1 | 0 | 0 |
| Sample 2 | 197.5 | >0,<250 |
| Sample 3 | 249.6 | 250 |
| Sample No. 4 | 379.3 | >250,<500 |
| Sample No. 5 | 503.4 | 500 |
| Sample No. 6 | 755.1 | >500,<1000 |
| Sample 7 | 997.6 | 1000 |
Table 4 example 4 test results vs experimental results:
| sample number | Titration method (mg/L) | Indicator card of example 4 (mg/L) |
| Sample 1 | 0 | 0 |
| Sample 2 | 198.7 | >0,<250 |
| Sample 3 | 247.8 | 250 |
| Sample No. 4 | 377.2 | >250,<500 |
| Sample No. 5 | 499.5 | 500 |
| Sample No. 6 | 802.2 | >500,<1000 |
| Sample 7 | 1002.3 | 1000 |
Table 5 example 5 test results vs experimental results:
| sample number | Titration method (mg/L) | Indicator card of example 5 (mg/L) |
| Sample 1 | 0 | 0 |
| Sample 2 | 201.3 | >0,<250 |
| Sample 3 | 250.5 | 250 |
| Sample No. 4 | 402.3 | >250,<500 |
| Sample No. 5 | 501.3 | 500 |
| Sample No. 6 | 802.2 | >500,<1000 |
| Sample 7 | 1002.3 | 1000 |
Table 6 example 6 test results vs experimental results:
| sample number | Titration method (mg/L) | Indicator card of example 6 (mg/L) |
| Sample 1 | 0 | 0 |
| Sample 2 | 151.9 | >0,<250 |
| Sample 3 | 298.9 | 250 |
| Sample No. 4 | 352.1 | >250,<500 |
| Sample No. 5 | 497.8 | 500 |
| Sample No. 6 | 701.6 | >500,<1000 |
| Sample 7 | 998.1 | 1000 |
Table 7 example 7 test results vs experimental results:
analysis of the above test results:
the test result is matched with the sample detection concentration measured by a titration method in the technical standard of the applied disinfectant, especially the content measurement of the part close to the end value of the indicator card is very close to the content measurement of the part close to the end value of the indicator card by the titration method, and the detection accuracy of the indicator card is very high.
The present invention is not limited to the above embodiments, and those skilled in the art can implement the present invention in other embodiments according to the disclosure of the present invention, or make simple changes or modifications on the design structure and idea of the present invention, and fall into the protection scope of the present invention.
Claims (10)
1. A mother liquor for manufacturing a quaternary ammonium salt disinfectant concentration detection chemical indicator card is characterized in that: the mother solution comprises a pH buffer solution, a leveling agent, a color fixing agent and a color-changing reactive substance capable of reacting with quaternary ammonium salt cations, wherein the pH of the mother solution is 2.8-3.8, a buffer pair substance of the pH buffer solution is citric acid and disodium hydrogen phosphate, the color-changing reactive substance is bromophenol blue or bromocresol green, the mass fraction of the color-changing reactive substance in the mother solution is 0.03-0.1%, a solvent is 95% ethanol by volume, the mass fraction of 95% ethanol in the mother solution is 19-20%, the mass fraction of the leveling agent in the mother solution is 0.1-1%, and the mass fraction of the color fixing agent in the mother solution is 3.2%.
2. The mother liquor for manufacturing the chemical indicator card for detecting the concentration of the quaternary ammonium salt disinfectant as claimed in claim 1, wherein: the color-changing reactive substance comprises bromophenol blue or bromocresol green, when the bromophenol blue is selected as the color-changing reactive substance, the pH of the mother liquor is adjusted to be 2.8-3.0 through a pH buffer solution, and when the bromocresol green is selected as the color-changing reactive substance, the pH of the mother liquor is adjusted to be 3.6-3.8 through the pH buffer solution.
3. The mother liquor for manufacturing the chemical indicator card for detecting the concentration of the quaternary ammonium salt disinfectant as claimed in claim 1, wherein: the leveling agent is peregal O-25 or peregal O-20.
4. The mother liquor for manufacturing the chemical indicator card for detecting the concentration of the quaternary ammonium salt disinfectant as claimed in claim 1, wherein: the color fixing agent is polyethylene glycol and polyvinylpyrrolidone (PVP K30), and the mass fractions of the color fixing agent and the polyvinylpyrrolidone in the mother liquor are 1.2% and 2%, respectively.
5. A preparation method of a chemical indicator card for detecting the concentration of a quaternary ammonium salt disinfectant is characterized by comprising the following preparation steps:
s1: dissolving a color-changing active substance and a leveling agent by using 95% ethanol in volume fraction to obtain a solution A, wherein the mass fractions of the color-changing active substance and the leveling agent are respectively 0.03% -0.1% and 0.1% -1% of a mother solution, and the mass fraction of 95% ethanol in the mother solution is 19% -20%;
s2: preparing a pH buffer solution:
preparing pH buffer solutions from anhydrous citric acid and disodium hydrogen phosphate by using deionized water respectively;
s3: sequentially dissolving polyethylene glycol and polyvinylpyrrolidone (PVP K30) in a pH buffer solution with a formula amount to ensure that the mass fractions of the polyethylene glycol and the polyvinylpyrrolidone in the mother solution are 1.2% and 2% respectively to obtain a solution B;
s4: uniformly mixing the solution A and the solution B to obtain mother liquor, wherein the pH of the mother liquor is 2.8-3.8;
s5: soaking absorbent paper in the mother liquor for 10-15min, and drying at 55-70 ℃ to obtain absorbent paper containing the color-changing reactive active substances;
s6: and (5) fixing the absorbent paper of S5 on a carrier card to obtain the quaternary ammonium salt disinfectant concentration indication card.
6. The method for preparing a chemical indicator card for detecting the concentration of quaternary ammonium salt disinfectant as claimed in claim 5, wherein the chemical indicator card comprises: when the color-changing active substance is bromophenol blue, the pH of the mother liquor is adjusted to 2.8-3.0 by a pH buffer solution.
7. The method for preparing a chemical indicator card for detecting the concentration of quaternary ammonium salt disinfectant as claimed in claim 6, wherein the chemical indicator card comprises: the anhydrous citric acid and the disodium hydrogen phosphate are respectively prepared into 0.1mol/L and 0.2mol/L solutions by deionized water; 0.2mol/L disodium hydrogen phosphate solution and 0.1mol/L citric acid solution are mixed according to the volume ratio of 2.18:17.82 to prepare pH buffer solution with the pH value of 2.6.
8. The method for preparing a chemical indicator card for detecting the concentration of quaternary ammonium salt disinfectant as claimed in claim 5, wherein the chemical indicator card comprises: and when the color-changing reaction active substance is bromocresol green, adjusting the pH of the mother solution to 3.6-3.8 by using a pH buffer solution.
9. The method for preparing a chemical indicator card for detecting the concentration of quaternary ammonium salt disinfectant according to claim 8, wherein the chemical indicator card comprises: the anhydrous citric acid and the disodium hydrogen phosphate are respectively prepared into 0.1mol/L and 0.2mol/L solutions by deionized water; 0.2mol/L disodium hydrogen phosphate solution and 0.1mol/L citric acid solution are mixed according to the volume ratio of 6.44:13.56 to prepare pH buffer solution with the pH value of 3.6.
10. The test paper for detecting the concentration of quaternary ammonium salt disinfectant or the chemical indicator card for detecting the concentration of quaternary ammonium salt disinfectant, which is obtained by the method for preparing the chemical indicator card for detecting the concentration of quaternary ammonium salt disinfectant according to any one of claims 5 to 9.
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Citations (7)
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2021
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| US3873272A (en) * | 1974-02-20 | 1975-03-25 | Baxter Laboratories Inc | Reagent and method for albumin determination |
| JPH01274061A (en) * | 1988-04-27 | 1989-11-01 | Terumo Corp | Test piece and testing tool for measurment of chloride |
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| JPH07140131A (en) * | 1993-11-19 | 1995-06-02 | Permachem Asia Ltd | Concentration control method for benzalkonium chloride disinfectant |
| JP2002243719A (en) * | 2001-02-14 | 2002-08-28 | Permachem Asia Ltd | Indicator and method for quantitative determination of bactericide |
| CN104198480A (en) * | 2014-08-20 | 2014-12-10 | 浙江大学 | Aqueous quaternary ammonium salt detection test paper and preparation method thereof |
| CN105300973A (en) * | 2015-10-27 | 2016-02-03 | 广东环凯微生物科技有限公司 | Rapid detection reagent and method for concentration of quaternary ammonium salt |
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