CN113913447A - SARS-CoV-2 full length cDNA clone single copy plasmid and its construction method - Google Patents
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Abstract
The invention relates to a SARS-CoV-2 full-length cDNA clone single copy plasmid and its construction method, concretely, combining In-Fusion and Gibson Assembly seamless clone to make full-length cDNA synthesis and clone for SARS-CoV-2 genome. A single copy plasmid pBac-mini (named pY1B at the early stage and named pBacm at present) with stable replication and smaller molecular weight based on a BAC vector is constructed, the full-length cDNA of SARS-CoV-2 is cloned to the vector, a recombinant single copy plasmid pBacm-SARS-CoV-2 is constructed, and the Recombineering technology is utilized to carry out beneficial base modification, gene knockout and other genetic engineering modifications on the full-length cDNA.
Description
Technical Field
The invention belongs to the field of biotechnology, and particularly relates to a SARS-CoV-2 full-length cDNA clone single copy plasmid and a construction method thereof.
Background
At present, new coronary pneumonia[1]The infectious diseases of the second class are regulated by infectious disease prevention and treatment laws of the people's republic of China, are managed according to the infectious diseases of the first class, have the characteristics of strong infectivity, long incubation period, complex transmission path and the like, and the clinical signs of infected patients are manifested as fever, respiratory symptoms, serious respiratory diseases and pneumonia[2]。
SARS-CoV-2 is a single-stranded positive-stranded RNA virus, with a genome length of about 29.8kb, possessing the largest genome among known RNA viruses. The SARS-CoV-2 genome structure follows the known specific gene characteristics of Coronaviruses (CoVs), with a cap structure at the 5 'end and a polyadenylated tail at the 3' end. The SARS-CoV-2 genome contains 10 Open Reading Frames (ORFs), and may co-encode at least 29 proteins[3]. The regions located at the 5' end of the genome and more than two thirds are ORF1 and ORF1b, and the translation generates two large overlapping polyproteins pp1a and pp1ab, the latter is translated by a-1-position ribosome frameshift mechanism, and is cleaved by virus-encoded proteases (Main protease and Papain-like protease) to generate 16 non-structural proteins (named NSP 1-NSP 16 respectively), the assembly releases replication-transcription complexBody[4]. The third region located at the 3' end of the genome encodes 4 viral structural proteins, including Spike glycoprotein (S), Envelope protein (E), Membrane protein (M) and Nucleocapsid protein (N). In addition, the SARS-CoV-2 genome 3 'end also encodes at least 6-9 species-specific accessory proteins unique to each CoV, ORF3a, ORF6, ORF7a, ORF7b, ORF8, ORF9b and ORF10, respectively, expressed from a series of Subgenomic mRNAs (sgmRNAs) at the 3' end[5]. Some of these accessory proteins assemble on the viral particle, e.g. 3a is found as a structural protein in SARS-CoV[6]3a number of molecules in SARS-CoV-2, which is equivalent to the structural protein M with the highest abundance of molecules[7]。
Because the genome of the coronavirus is the largest known genome in the RNA virus, the replicase gene carried by the coronavirus has instability when the replicase gene is propagated in bacteria in a cDNA clone form, and the difficulty in synthesizing large genome full-length cDNA in vitro is high, the construction of the full-length infectious cDNA of the coronavirus is greatly hindered[8]. To overcome these difficulties, three innovative research approaches different from the traditional approach to establish a reverse genetics system for coronaviruses have been established, including the use of Bacterial Artificial Chromosomes (BACs)[9]In vitro ligation of cDNA fragments[10]And propagating full-length cDNA of coronavirus using poxvirus as vector[11]。
After the SARS-CoV-2 virus appears and causes the COVID-19 epidemic situation, various variant strains continuously appear in the propagation of various countries and regions in the world. Some of these Variants have enhanced transmission and virulence and develop escape mutations, and are defined by the world health organization as Variants of cancer (VOC) that need close attention. The research on the function, the transmissibility, the pathogenicity and the molecular mechanism of the immune escape of the mutant gene and the gene product of the VOC virus strain provides a theoretical basis for the discovery of the target of the antiviral drug and the design and the research and the development of the antibody drug and the vaccine of the immune escape strain. The reverse genetics technology platform plays an irreplaceable role in basic research and application research, is also a basic tool for researching new coronavirus fulminant infectious disease pathogens SARS-CoV, MERS-CoV and SARS-CoV-2, and has been published in a large number of documents. If the attenuated gene is found, the virus which is based on the prototype strain framework with weak transmission and is further attenuated is constructed, the spike protein S is modified, a safe strain can be provided for the production of the inactivated vaccine, the safety risk to the production personnel and the environment of the inactivated vaccine is reduced, and the virus is a new generation vaccine aiming at immune escape strains.
In order to better research the structure and function of SARS-CoV-2 genome and better respond to various variant strains which are continuously appeared at present, the invention adopts a BAC vector and a homologous recombination seamless cloning method (combining In-Fusion, Gibson Assembly and Recombineering)[12]Three techniques), the full-length cDNA clone plasmid of SARS-CoV-2 is quickly constructed. This provides a new idea for the subsequent study of SARS-CoV-2 and the construction of full-length cDNA clone of large genome such as other coronavirus.
Reference documents:
1.Sun P,Lu X,Xu C,et al.Understanding of COVID-19based on current evidence[J].J Med Virol.2020；92(6):548-551.
2.Jin Y,Yang H,Ji W,et al.Virology,epidemiology,pathogenesis,and control of COVID-19[J].Viruses.2020；12(4):372.
3.Almazán F,Dediego ML,Galán C,et al.Construction of a severe acute respiratory syndrome coronavirus infectious cDNA clone and a replicon to study coronavirus RNA synthesis[J].J Virol.2006；80(21):10900-10906.
4.Khailany RA,Safdar M,Ozaslan M.Genomic characterization of a novel SARS-CoV-2[J].Gene Rep.2020；19:100682.
5.Kirtipal N,Bharadwaj S,Kang SG.From SARS to SARS-CoV-2,insights on structure,pathogenicity and immunity aspects of pandemic human coronaviruses[J].Infect Genet Evol.2020；85:104502.
6.Shen S,Lin PS,Chao YC,et al.The severe acute respiratory syndrome coronavirus 3a is a novel structural protein[J].Biochem Biophys Res Commun.2005；330(1):286-292.
7.Zhang XY,Guo J,Wan X,et al.Biochemical and antigenic characterization of the structural proteins and their post-translational modifications in purified SARS-CoV-2virions of an inactivated vaccine candidate[J].Emerg Microbes Infect.2020；9(1):2653-2662.
8.Almazán F,Sola I,
S,et al.Coronavirus reverse genetic systems:infectious clones and replicons[J].Virus Res.2014；189:262-270.
9.Almazán F,González JM,Pénzes Z,et al.Engineering the largest RNA virus genome as an infectious bacterial artificial chromosome[J].Proc Natl Acad Sci U S A.2000；97(10):5516-5521.
10.Baric RS,Yount B.Subgenomic negative-strand RNA function during mouse hepatitis virus infection[J].J Virol.2000；74(9):4039-4046.
11.Thiel V,Herold J,Schelle B,et al.Infectious RNA transcribed in vitro from a cDNA copy of the human coronavirus genome cloned in vaccinia virus[J].J Gen Virol.2001；82(6):1273-1281.
12.Yu D,Ellis HM,Lee EC,et al.An efficient recombination system for chromosome engineering in Escherichia coli[J].Proc Natl Acad Sci U S A.2000；97(11):5978-5983.
disclosure of Invention
The invention firstly relates to a full-length cDNA clone single-copy plasmid pBacm-T7p-SARS-CoV-2-FL-T7T of SARS-CoV-2 vaccine strain, the size is about 36.4kb, and the nucleotide sequence is shown in SEQ ID NO. 1. Wherein, the sequence of SARS-CoV-2 is shown as SEQ ID NO.2, and the sequence of pBacm vector is shown as SEQ ID NO. 3.
The invention also relates to a construction method of the pBacm-T7p-SARS-CoV-2-FL-T7T plasmid, which comprises the following steps:
(1) constructing a pBacm vector and modifying enzyme cutting sites added on two sides of the pBacm vector;
(2) constructing tool plasmids pBacm-SacB-Kan and pBacm-SacB-Amp containing forward and reverse screening markers;
(3) constructing a full-length cDNA single-copy plasmid of the SARS-CoV-2 vaccine strain;
(4) connecting the SARS-CoV-2 full-length genome sequence with the single copy carrier pBacm to obtain pBacm-SARS-CoV-2- (FL &2GC) plasmid;
(5) repairing redundant GC bases of a site in the pBacm-SARS-CoV-2- (FL &2GC) recombinant plasmid, and simultaneously cloning a T7 promoter sequence and a T7 terminator sequence into the single-copy vector to obtain a target vector pBacm-T7 p-SARS-CoV-2-FL-T7T.
The specific steps of the step (1) comprise:
1) taking pBacm plasmid as a template, reversely amplifying the large plasmid ring through PCR, and respectively introducing NotI and AscI enzyme cutting sites at the 5 'end and the 3' end of an amplification product;
2) using pY44 plasmid as a template, amplifying a kala antibiotic gene (Kan) by PCR, and respectively introducing restriction enzyme AscI and NotI enzyme cutting sites at the 5 'end and the 3' end of an amplification product;
3) the PCR products in 1) and 2) were purified, digested with AscI and NotI, and ligated with T4 DNA ligase to obtain pBacm-AscI-Kan-NotI plasmid.
The specific steps of the step (2) comprise:
1) designing a primer to amplify a sucrose lethal gene SacB by taking a pK18mob-SacB plasmid as a template, wherein the 5 'end and the 3' end of the amplification primer respectively contain AscI enzyme cutting sites and NotI enzyme cutting sites;
2) purifying the SacB gene PCR product, and then carrying out enzyme digestion by AscI and NotI;
3) digesting the constructed pBacm-AscI-Kan-NotI plasmid by AscI and NotI;
4) purifying the enzyme digestion products in 2) and 3), and then connecting with T4 DNA ligase to obtain a recombinant plasmid pBacm-SacB;
5) respectively taking pY44 and pBR322 plasmids as templates, designing primers to amplify Kan and ampicillin (Amp) genes, wherein the 5 'end and the 3' end of the amplification primers respectively contain 50bp sequences which are homologous with the two sides of the end of the SacB gene in pBacm-SacB;
6) after the PCR product of the resistance gene in 5) is purified, the Kan or Amp gene is respectively inserted into a pBacm-SacB vector to obtain recombinant plasmids pBacm-SacB-Kan and pBacm-SacB-Amp.
The specific steps of the step (3) comprise:
1) extracting SARS-CoV-2 inactivated vaccine strain RNA and reverse transcribing into cDNA;
2) using the cDNA as a template, designing primers to carry out PCR amplification respectively to obtain 17 gene segments of the SARS-CoV-2 inactivated vaccine strain, wherein each gene segment contains a homologous sequence of 15 bp; in addition, the locus T of 8791 and the locus T of 12655 are respectively synonymously mutated into C, and two BglI endonuclease sequences are introduced to be used as genetic markers;
3) the gene fragments in 2) are respectively connected to pUC19 vector which is cut by KpnI and HindIII by taking the length of every 5kb as a unit, so as to obtain 6 recombinant plasmids;
4) using the 6 recombinant plasmids as templates, designing primers to perform PCR amplification respectively to obtain 6 SARS-CoV-2 genome fragments with each length of about 5 kb;
5) the 6-segment gene fragment in 4) is gradually connected to the linearized pBR322 vector by 3 times, and 10kb of each connection is carried out to obtain recombinant plasmids containing SARS-CoV-210 kb, 20kb and full-length 30kb respectively.
The invention has the beneficial effects that:
aiming at SARS-CoV-2 inactivated vaccine strain, the invention adopts a method based on minimum single copy plasmid pBacm, homologous recombination seamless cloning (In-Fusion, Gibson Assembly) and Recombineering gene editing to quickly and conveniently obtain the full-length cDNA clone plasmid pBacm-T7p-SARS-CoV-2-FL-T7T of SARS-CoV-2 vaccine strain. The SARS-CoV-2 full-length cDNA clone plasmid constructed based on the method can be applied to the deep research of the genetic variation of SARS-CoV-2, virulence genes, virus infection and pathogenic mechanism, and can also provide a powerful technical platform for vaccine development. The method is also suitable for constructing SARS-CoV-2 replicon plasmid, and can be used for screening antiviral drugs and researching the functions of novel coronavirus genomes. The method can also be widely applied to cDNA construction work of other small genome RNA viruses such as enterovirus, measles virus and the like. The invention has the following advantages:
firstly, pBeloBAC11 plasmid (7.5 kb, which is the BAC vector with the minimum molecular weight accepted at present) is modified, redundant sequences are deleted on the premise of not influencing the function of the vector, so that the plasmid becomes a single copy plasmid pBacm (6.4 kb) with smaller molecular weight, and the molecular biological operation such as PCR amplification and the like is easy to carry out.
Secondly, the full-length cDNA of SARS-CoV-2 is connected with pBacm single copy plasmid, the virus large genome sequence can keep stable and not easy to mutate, and exists in the body of bacteria in a ring structure, the conversion efficiency is high, and the resolution, the separation and the purification are easy.
Again, pBacm plasmids can be further modified, such as by the addition of single cleavage sites (e.g., AscI and NotI), to facilitate the shuttling of the viral full-length cDNA from a single copy plasmid to a low copy (e.g., pBR22) or multicopy plasmid, to facilitate mass production and extraction of subsequent plasmids.
Finally, the Recombineering technology can be used for quickly and conveniently editing genes or bases of SARS-CoV-2 full-length cDNA on the pBacm single-copy plasmid, such as knockout and knock-in of any gene and base, so that the gene functions of the virus can be researched, for example, the construction of SARS-CoV-2 replicon containing a reporter gene and similar viral vectors, the construction of inactivated vaccine strains based on attenuated strain frameworks, and the research of recombinant proteins and virus-like particle vaccines based on viral vectors. .
Drawings
FIG. 1 is a schematic diagram of a pBacm single copy plasmid construction strategy with smaller molecular weight.
FIG. 2 is a schematic diagram of a pBacm single copy plasmid modification strategy containing a single restriction enzyme site.
FIG. 3, schematic diagram of pBacm-SacB-Kan/Amp single copy plasmid construction strategy.
FIG. 4 is a schematic diagram of the SARS-CoV-2 full-length cDNA cloning strategy.
FIG. 5, In Fusion (A) and Gibson Assembly (B) are schematic representations of the ligation (first 5kb and first 10kb ligations, respectively).
FIG. 6 shows the results of the restriction enzyme identification electrophoresis of the recombinant plasmids cloned in pBR322 vector (A) and pBacm vector (B)
In fig. 6A:
m: the leftmost side is DNA marker DL 15000 and the rightmost side is DNA marker DL 5000.
1: pBR322-SARS-CoV-2- (1-9975) recombinant plasmid.
2: the pBR322-SARS-CoV-2- (1-9975) recombinant plasmid is cut into product by AscI and Not I.
3: pBR322-SARS-CoV-2- (1-19975&1GC) recombinant plasmid.
4: the pBR322-SARS-CoV-2- (1-19975&1GC) recombinant plasmid is digested with AscI and Not I to obtain the product.
5: pBR322-SARS-CoV-2- (FL &2GC) recombinant plasmid.
6: the pBR322-SARS-CoV-2- (FL &2GC) recombinant plasmid is cut into products by AscI and Not I.
In fig. 6B:
M:DNA marker DL 15000。
1 pBacm-SARS-CoV-2- (FL &2GC) recombinant plasmid.
2, the product of pBacm-SARS-CoV-2- (FL &2GC) after AscI and Not I enzyme digestion.
FIG. 7, SARS-CoV-2 full-length cDNA 9975nt (A) and 19975nt (B) repair sequencing results
In the 7A and 7B diagrams, the first row gene sequences are respectively the SARS-CoV-2 genome 9967-9984nt and 19965-19982nt amplified by taking the SARS-CoV-2 vaccine strain cDNA as a template, the second row gene sequences are respectively the SARS-CoV-2 genome 9967-9986nt and 19965-19984nt in the pBR322-SARS-CoV-2- (FL &2GC) recombinant plasmid, and the third row gene sequences are respectively the SARS-CoV-2 genome 9967-9984nt and 19965-19982nt in the pBacm-T7p-SARS-CoV-2-FL-T7T recombinant plasmid.
FIG. 8, pBacm-T7p-SARS-CoV-2-FL-T7T recombinant plasmid in SARS-CoV-2 genome 8791nt and 12655nt BglI enzyme cutting site (genetic marker) validation electrophoresis results, in which:
M:DNA marker DL 5000。
1: 7945-9655 fragment of SARS-CoV-2 was amplified using pBacm-T7p-SARS-CoV-2-FL-T7T as template.
2: 1, the PCR fragment is cut by Bgl I enzyme.
3: the 11419-13298 fragment of SARS-CoV-2 was amplified using pBacm-T7p-SARS-CoV-2-FL-T7T as a template.
4: the PCR fragment of 2 is cut by Bgl I enzyme.
5: 7945-9655 fragment of SARS-CoV-2 amplified using vaccine strain cDNA as template.
6: 5, the PCR fragment is cut by Bgl I enzyme.
7: 11419-13298 fragment of SARS-CoV-2 amplified by using vaccine strain cDNA as template.
8: 7, the PCR fragment is cut by Bgl I.
FIG. 9, a schematic diagram of the complete structure of pBacm-T7p-SARS-CoV-2-T7T single copy plasmid.
Detailed Description
Example 1: construction of single copy plasmid pBacm with smaller molecular weight and modification containing single enzyme cutting site
The pBacm plasmid construction strategy is shown in figures 1 and 2, and the specific steps are as follows:
(1) backward PCR amplification was performed using pBeloBAC11 plasmid (purchased from Biovector plasmid vector strain cell Gene Collection) as a template and primers (SEQ ID NO.4 and NO.5), the product sequence was shown in SEQ ID NO.3, and both the 5 'and 3' ends contained PacI cleavage sites.
(2) The PCR product was purified, digested with PacI, and ligated with T4 DNA ligase to obtain pBacm plasmid (. about.6.4 kb).
(3) The pBacm plasmid is used as a template, the plasmid macrocycle is reversely amplified through PCR primers (SEQ ID NO.7 and NO.8), the product sequence is shown as SEQ ID NO.6, and the 5 'end and the 3' end of the product respectively contain NotI and AscI enzyme cutting sites.
(4) Using pY44 plasmid as template, amplifying kala antibiotic gene (Kan, SEQ ID NO.9) by PCR primer (SEQ ID NO.10 and NO.11), 5 'end and 3' end respectively containing AscI and NotI enzyme cutting site.
(5) The PCR products in (3) and (4) were purified, digested simultaneously with AscI and NotI, and ligated to each other to obtain pBacm-AscI-Kan-NotI plasmid containing cleavage sites at both ends.
Example 2: construction of screening tool plasmid pBacm-SacB-Kan/Amp
The construction strategy of the plasmid pBacm-SacB-Kan/Amp is shown in figure 3, and the specific steps are as follows:
(1) a sucrose lethal gene SacB (SEQ ID NO.12) is amplified by using pK18mob-SacB plasmid (purchased from Biovector plasmid vector strain cell gene collection center) as a template and designing a 5 'end and a 3' end which respectively contain AscI and NotI enzyme cutting sites (SEQ ID NO.13 and NO. 14).
(2) The SacB gene PCR product was purified and digested with AscI and NotI.
(3) The constructed pBacm-AscI-Kan-NotI plasmid was digested with AscI and NotI.
(4) And (3) purifying the enzyme digestion products in the steps (2) and (3), and then connecting the enzyme digestion products by using T4 DNA ligase to obtain the recombinant plasmid pBacm-SacB.
(5) Primers (Kan: SEQ ID NO.16 and NO. 17; Amp: SEQ ID NO.19 and NO.20) were designed to amplify the genes Kan (SEQ ID NO.15) and ampicillin (Amp, SEQ ID NO.18) using pY44 and pBR322 plasmids as templates, respectively.
(6) The clearly recovered Kan or Amp resistance gene was shock-transformed into DY380 competent cells containing pBacm-SacB plasmid. The process needs to involve the preparation of DY380 competent cells containing recombinant plasmid[12]The specific preparation process is as follows:
(a) the constructed pBacm-SacB plasmid is click-transformed into DY380 competent cells, and the DY380 competent cells are spread on LB solid plates containing chloramphenicol (Chl) and are inverted at 30 ℃ for overnight culture.
(b) Individual colonies on the plates were picked and plated in 2mL LB liquid medium containing Chl and shake-cultured at 30 ℃.
(c) 200 μ L of DY380 bacterial liquid containing pBacm-SacB plasmid was transferred to fresh 11mL LB (Chl) liquid medium and shake-cultured at 30 ℃.
(d) When OD of the bacterial liquid is 0.6-0.9, putting the bacterial liquid into a rotary shaking water bath shaking table, inducing at 42 ℃ for 15min, then quickly putting the bacterial liquid on ice, putting an ice box into the shaking table, and rotationally shaking the ice box for 10 min.
(e) Taking out the bacterial liquid, placing in a precooled 4 ℃ centrifuge for centrifugation, 5500 Xg, 8 min.
(f) The supernatant was discarded, and the pellet was vortex-washed with pre-cooled 20mL sterile water, then centrifuged at 5500 Xg for 8min at 4 ℃ and the washing step was repeated 1 time.
(g) The cells were resuspended in 100. mu.L of sterile water to prepare DY380 electroporation competent cells.
(h) The clearly recovered Kan or Amp PCR product is transformed into DY380 competent cells containing pBacm-SacB plasmid by electric shock, spread on LB solid plate containing Chl antibiotic, and cultured overnight by inversion at 30 ℃. The positive clone is selected to carry out colony PCR and extract plasmids, thus obtaining recombinant tool plasmids pBacm-SacB-Kan and pBacm-SacB-Amp.
Example 3: acquisition of cDNA for SARS-CoV-2
The RNA of the stock solution of SARS-CoV-2 inactivated vaccine (see CN202010559132.3) developed by Wuhan Biopreparation research institute was extracted with QIAamp Viral RNA Mini Kit (available from QIAGEN Co.), and purified by PrimeScriptTMThe Double Strand cDNA Synthesis Kit (purchased from Takara) reverse-transcribed cDNA to obtain cDNA, which was used as a template for subsequent full-length PCR amplification.
Example 4: SARS-CoV-2 full-length 17 gene fragment amplification
Using the cDNA of example 3 as a template, the primers were each separated by the Phusion enzyme (available from NEB corporation):
a to F: SEQ ID NO.38 and A-R: SEQ ID NO.39,
B-F: SEQ ID NO.40 and B-R: SEQ ID NO.41,
C-F: SEQ ID NO.42 and C-R: SEQ ID NO.43,
D-F: SEQ ID NO.44 and D-R: SEQ ID NO.45,
E-F: SEQ ID NO.46 and E-R: SEQ ID NO.47,
F to F: SEQ ID NO.48 and F-R: SEQ ID NO.49,
G-F: SEQ ID NO.50 and G-R: SEQ ID NO.51,
H-F: SEQ ID NO.52 and H-R: SEQ ID NO.53,
I-F: SEQ ID No.54 and I-R: SEQ ID NO.55,
J-F: SEQ ID NO.56 and J-R: SEQ ID NO.57,
K-F: SEQ ID NO.58 and K-R: SEQ ID NO.59,
L-F: SEQ ID NO.60 and L-R: SEQ ID NO.61,
M-F: SEQ ID NO.62 and M-R: SEQ ID NO.63,
N-F: SEQ ID NO.64 and N-R: SEQ ID NO.65,
O-F: SEQ ID NO.66 and O-R: SEQ ID NO.67,
P-F: SEQ ID NO.68 and P-R: SEQ ID NO.69,
Q-F: SEQ ID NO.70 and Q-R: PCR amplification was performed with SEQ ID NO. 71.
17 small SARS-CoV-2 genomic fragments were obtained, which were designated:
gene fragment A: SEQ ID NO.21,
Gene fragment B: SEQ ID NO.22,
Gene fragment C: SEQ ID NO.23,
Gene fragment D: SEQ ID NO.24,
Gene fragment E: SEQ ID NO.25,
Gene fragment F: SEQ ID NO.26,
Gene fragment G: SEQ ID NO.27,
Gene fragment H: SEQ ID NO.28,
Gene fragment I: SEQ ID NO.29,
Gene fragment J: SEQ ID NO.30,
Gene fragment K: SEQ ID NO.31,
Gene fragment L: SEQ ID NO.32,
Gene fragment M: SEQ ID NO.33,
Gene fragment N: SEQ ID NO.34,
Gene fragment O: SEQ ID NO.35,
Gene fragment P: SEQ ID NO.36 and Gene fragment Q: SEQ ID NO. 37.
Wherein 5 'end of the gene fragment A, D, H, J, M, P and 3' end of pUC19 vector digested with KpnI and HindIII have 15bp homologous sequence, and 3 'end of the gene fragment C, G, I, L, O, Q and 5' end of pUC19 vector digested with KpnI and HindIII have 15bp homologous sequence. It is worth mentioning that, in order to distinguish from wild type SARS-CoV-2 strain, 1 synonymous mutation is introduced into each of the full length 8791 and 12655 sites, and 1 BglI cleavage site is generated respectively, and is used as genetic marker, so as to confirm that the strain rescued in the subsequent transfection experiment is artificially synthesized, but not caused by wild strain contamination. The results of the verification of the Bgl I cleavage site are shown in FIG. 8.
Example 5: construction of recombinant plasmid set containing SARS-CoV-2 Gene fragment of about 5kb
Each PCR product amplified In example 4 was ligated to Kpn I and Hind III digested pUC19 (. about.2.6 kb) vectors (incubated at 50 ℃ for 15min) In a mixture of A + B + C, D + E + F + G, H + I, J + K + L, M + N + O and P + Q using In-Fusion HD Cloning kit (available from Takara Co.), as shown In FIG. 5A. Wherein the molar concentration ratio of each gene fragment to the vector is 2: 1. the In-Fusion ligation product was then transformed into Stellar component Cell (available from Takara), plasmids were extracted from positive clones, and the correct plasmids were sequenced by restriction enzyme digestion and sent to Shanghai Biometrics, Inc. The 6 recombinant plasmids containing 5kb SARS-CoV-2cDNA fragment were designated as pUC19-I, pUC 19-II, pUC 19-III, pUC 19-IV, pUC 19-V and pUC 19-VI, respectively.
Example 6: construction of recombinant plasmid pBR322-SARS-CoV-2- (FL &2GC) containing target Gene of about 30kb
Using 6 recombinant plasmids containing SARS-CoV-2cDNA fragments of about 5kb as templates in example 5, primer pairs were used
I-F: SEQ ID No.78 and I-R: SEQ ID NO.79,
II-F: SEQ ID NO.80 and II-R: SEQ ID NO.81,
III-F: SEQ ID NO.82 and III-R: SEQ ID NO.83,
IV-F: SEQ ID NO.84 and IV-R: SEQ ID NO.85,
V-F: SEQ ID NO.86 and V-R: SEQ ID NO.87,
VI-F: SEQ ID NO.88 and VI-R: the PCR amplification was carried out for SEQ ID NO.89, respectively.
The resulting 6 SARS-CoV-2PCR products of about 5kb were each referred to as
Gene fragment I: SEQ ID NO.72,
Gene fragment II: SEQ ID NO.73,
Gene fragment III: SEQ ID NO.74,
Gene fragment IV: SEQ ID NO.75,
Gene fragment v: SEQ ID NO.76,
Gene fragment VI: SEQ ID NO. 77.
Combining gene segments I and II, and PCR primer pair pBR 322-F: SEQ ID NO.91 and pBR 322-R: the Gibson Assembly ligation (kit from invitrogen) was performed with the pBR322 linearized vector (SEQ ID NO.92, SEQ ID NO. 90) back-amplified to give a recombinant plasmid of approximately 13kb in length, designated pBR322-SARS-CoV-2- (1-9975), as shown in FIG. 5B. The plasmid is subjected to NotI enzyme digestion and CIP dephosphorylation treatment, gel cutting recovery and purification, and then mixed with III and IV to carry out a second round of Gibson Assembly connection, so as to obtain a recombinant plasmid with the length of about 23kb, which is named pBR322-SARS-CoV-2- (1-19975&1 GC). The recombinant plasmid is subjected to NotI enzyme digestion and CIP dephosphorylation treatment, gel cutting recovery and purification, and then combined with V and VI to be subjected to a third-round Gibson Assembly to obtain the recombinant plasmid with the length of about 33kb, which is named as pBR322-SARS-CoV-2- (FL &2 GC).
The molar concentration ratio of each gene fragment to the vector in each Gibson Assembly reaction system is 1: 1,
the reaction conditions are 37 ℃ for 5min, 75 ℃ for 20min and 60 ℃ for 30 min.
Each round of Gibson Assembly ligation products was ligated with sterile water in a 1: 5 ratio dilution and introduction into Electropore ElectroMaxMDH 10B cells (from Invitrogen) or One shot Shot TMTOP10 chemical company E.coli cells (from Invitrogen) by either electric shock transformation or chemical transformation. As shown in FIG. 5B, primer pairs are designed at 3 junctions, and clones positive for PCR identification are picked to extract recombinant plasmids, and sent to sequencing for production.
FIG. 6A shows the results of AscI/NotI cleavage electrophoresis of 3 recombinant plasmids.
Example 7: construction of pBacm-SARS-CoV-2- (FL &2GC)
The plasmid pBR322-SARS-CoV-2- (FL &2GC) (prepared in example 6) was cleaved with AscI and Not I, and the cleaved full-length genome was recovered by phenol chloroform extraction and isopropanol precipitation. The linearized SARS-CoV-2 full-length genome is connected with a pBacm plasmid (an amplification primer pair is SEQ ID NO.94 and NO.95, and the sequence of an amplification product is shown as SEQ ID NO. 93) which is subjected to PCR reverse amplification linearization by utilizing a Gibson Assembly Kit, the obtained recombinant plasmid is named as pBacm-SARS-CoV-2- (FL &2GC), and is transformed into a DY380 temperature sensitive competent cell by electric shock, a single colony is picked, and the culture is carried out overnight at 30 ℃. FIG. 6B shows the results of AscI/NotI cleavage electrophoresis of the recombinant plasmid.
Example 8: deletion of two GC bases
It should be noted that, when using recombination for gene editing, the single copy plasmid to be edited needs to be transformed to DY380 temperature-sensitive competent cell by electric shock. The two-step screening method is adopted to operate, namely, the sequence of the region to be edited is replaced by the (SacB + Kan) segment containing the positive screening marker and the negative screening marker, and then the (SacB + Kan) segment of the region is replaced by the correct sequence.
Since there are redundant GC bases at both positions 9975nt and 19975nt, it is necessary to repair one sequence first, and then repair the second sequence on the basis of the first sequence, so 4 rounds of recombination process are needed.
The details are described in the following, in which the excess GC base at the 9975 site is repaired first, and then the GC base at the 19975 site is repaired:
(1) the pBacm-SacB-Kan plasmid is used as a template, PCR amplification (SacB + Kan) fragments are carried out on SEQ ID NO:96 and SEQ ID NO:97 by a primer with 50bp homologous with SARS-CoV-2 gene, and clean recovery is carried out. The (SacB + Kan) PCR product was then shock transformed into DY380 competent cells containing the pBacm-SARS-CoV-2- (FL &2GC) plasmid (prepared as described in example 2), spread on LB solid plates containing Kan antibiotics, and cultured overnight at 30 ℃ in an inverted state. Positive colonies were picked and the recombinant plasmid pBacm-SARS-CoV-2- (SacB + Kan) -9975 was extracted.
(2) The cDNA is taken as a template, and primers are designed for carrying out PCR amplification on the correct sequence (shown as SEQ ID NO. 98) of about 600bp around a position crossing 9975 on the SEQ ID NO.99 and the SEQ ID NO. 100. The correct sequence position is identical to the (SacB + Kan) -9975 position of the previous insertion. The correct sequence was transformed into DY380 competent cells containing pBacm-SARS-CoV-2- (SacB + Kan) -9975 plasmid by electric shock, spread on LB solid medium containing chloramphenicol (Chl) resistance and 10% sucrose, and cultured overnight at 30 ℃ by inversion. Positive clones were picked, cultured overnight at 30 ℃ with shaking, and then transferred to 500mL of LB liquid medium containing Chl for mass culture, to extract the recombinant plasmid pBacm-SARS-CoV-2- (FL &19975 GC).
(3) The pBacm-SacB-Kan plasmid is used as a template, PCR amplification (SacB + Kan) fragments are carried out on SEQ ID NO:101 and SEQ ID NO:102 through a primer with 50bp homologous SARS-CoV-2 genome, and clean recovery is carried out. The (SacB + Kan) PCR product was then shock-transformed into DY380 competent cells containing pBacm-SARS-CoV-2- (FL &19975GC) plasmid, plated on LB solid plate containing Kan antibiotic, and cultured overnight at 30 ℃ by inversion. Positive colonies were picked and the recombinant plasmid pBacm-SARS-CoV-2- (SacB + Kan) -19975 was extracted.
(4) The cDNA is taken as a template, and primers are designed for carrying out PCR amplification on the correct sequence (SEQ ID NO.103) of about 600bp around 19975 sites across SEQ ID NO.104 and SEQ ID NO. 105. The correct sequence position was identical to the (SacB + Kan) -19975 position of the previous step insertion. The correct sequence was transformed into DY380 competent cells containing pBacm-SARS-CoV-2- (SacB + Kan) -19975 plasmid by electric shock, plated on LB solid medium containing Chl antibiotic and 10% sucrose, and cultured overnight at 30 ℃ by inversion. The positive clones were picked, cultured overnight at 30 ℃ in a shaker, and then transferred to 500mL of LB liquid medium containing Chl for mass culture to extract recombinant plasmids. The plasmid thus obtained through the 4 rounds of recombination process was named pBacm-SARS-CoV-2-FL.
The sequencing results of the recombinant plasmids after knocking out 9975nt and 19975nt by using the Recombineering technology are shown in FIG. 7.
Example 9: insertion of the T7 promoter and T7 terminator
The T7 promoter sequence is inserted first, and then the T7 terminator sequence is repaired on the basis of the T7 promoter sequence, so 4 rounds of recombination editing process are needed. The whole process is similar to that of example 7, and will not be described in detail, and the following points are notable.
(1) The primer pairs of the PCR amplified (SacB + Kan) fragments are respectively
T7p-SacB-F SEQ ID NO.106 and T7p-Kan-R SEQ ID NO. 107;
or T7T-SacB-F SEQ ID NO.108 and T7T-Kan-R SEQ ID NO. 109.
The primer pairs for PCR amplification of correct replacement fragments are respectively
T7p-right-F SEQ ID NO.110 and T7p-right-R SEQ ID NO. 111;
or T7T-SacB-F SEQ ID NO.112 and T7T-Kan-R SEQ ID NO. 113.
The correct replacement sequences for the synthetic T7 promoter and T7 terminator are shown in SEQ ID NO.114 and SEQ ID NO.115, respectively.
(2) Knocking in the T7 promoter sequence, firstly, the (SacB + Kan) fragment amplified by the primer pair SEQ ID NO.106 and SEQ ID NO.107 is transformed into DY380 competent cells containing pBacm-SARS-CoV-2-FL plasmid, and the positive recombinant plasmid pBacm-SARS-CoV-2- (SacB + Kan) -T7p is identified. Then the T7 promoter sequence amplified by the primer pair SEQ ID NO.110 and SEQ ID NO.111 is transformed into DY380 competent cell containing pBacm-SARS-CoV-2- (SacB + Kan) -T7p by electric shock, and the positive recombinant plasmid pBacm-T7p-SARS-CoV-2 is identified. Then knocking in T7 terminator sequence, the operation steps are similar, firstly, the (SacB + Kan) fragment amplified by the primer pair SEQ ID NO.108 and SEQ ID NO.109 is transformed into DY380 competent cell containing pBacm-T7p-SARS-CoV-2 plasmid, and the positive recombinant plasmid pBacm-T7p-SARS-CoV-2- (SacB + Kan) -T7T is identified. Then the T7 terminator sequence amplified by the primer pair SEQ ID NO.112 and SEQ ID NO.113 is transformed into DY380 competent cells containing pBacm-T7p-SARS-CoV-2- (SacB + Kan) -T7T by electric shock, and the positive recombinant plasmid pBacm-T7p-SARS-CoV-2-T7T is obtained through identification.
Finally, it should be noted that the above embodiments are only used to help those skilled in the art understand the essence of the present invention, and are not used to limit the protection scope of the present invention.
Sequence listing
<110> Wuhan Biometrics institute of Biotechnology, Inc
<120> SARS-CoV-2 full length cDNA clone single copy plasmid and its construction method
<160> 115
<170> SIPOSequenceListing 1.0
<210> 1
<211> 36385
<212> DNA
<213> Artificial sequence ()
<400> 1
gtcgacagcg acacacttgc atcggatgca gcccggttaa cgtgccggca cggcctgggt 60
aaccaggtat tttgtccaca taaccgtgcg caaaatgttg tggataagca ggacacagca 120
gcaatccaca gcaggcatac aaccgcacac cgaggttact ccgttctaca ggttacgacg 180
acatgtcaat acttgccctt gacaggcatt gatggaatcg tagtctcacg ctgatagtct 240
gatcgacaat acaagtggga ccgtggtccc agaccgataa tcagaccgac aacacgagtg 300
ggatcgtggt cccagactaa taatcagacc gacgatacga gtgggaccgt ggtcccagac 360
taataatcag accgacgata cgagtgggac cgtggttcca gactaataat cagaccgacg 420
atacgagtgg gaccgtggtc ccagactaat aatcagaccg acgatacgag tgggaccatg 480
gtcccagact aataatcaga ccgacgatac gagtgggacc gtggtcccag tctgattatc 540
agaccgacga tacgagtggg accgtggtcc cagactaata atcagaccga cgatacgagt 600
gggaccgtgg tcccagacta ataatcagac cgacgatacg agtgggaccg tggtcccagt 660
ctgattatca gaccgacgat acaagtggaa cagtgggccc agagagaata ttcaggccag 720
ttatgctttc tggcctgtaa caaaggacat taagtaaaga cagataaacg tagactaaaa 780
cgtggtcgca tcagggtgct ggcttttcaa gttccttaag aatggcctca attttctcta 840
tacactcagt tggaacacga gacctgtcca ggttaagcac cattttatcg cccttataca 900
atactgtcgc tccaggagca aactgatgtc gtgagcttaa actagttctt gatgcagatg 960
acgttttaag cacagaagtt aaaagagtga taacttcttc agcttcaaat atcaccccag 1020
cttttttctg ctcatgaagg ttagatgcct gctgcttaag taattcctct ttatctgtaa 1080
aggctttttg aagtgcatca cctgaccggg cagatagttc accggggtga gaaaaaagag 1140
caacaactga tttaggcaat ttggcggtgt tgatacagcg ggtaataatc ttacgtgaaa 1200
tattttccgc atcagccagc gcagaaatat ttccagcaaa ttcattctgc aatcggcttg 1260
cataacgctg accacgttca taagcacttg ttgggcgata atcgttaccc aatctggata 1320
atgcagccat ctgctcatca tccagctcgc caaccagaac acgataatca ctttcggtaa 1380
gtgcagcagc tttacgacgg cgactcccat cggcaatttc tatgacacca gatactcttc 1440
gaccgaacgc cggtgtctgt tgaccagtca gtagaaaaga agggatgaga tcatccagtg 1500
cgtcctcagt aagcagctcc tggtcacgtt cattacctga ccatacccga gaggtcttct 1560
caacactatc accccggagc acttcaagag taaacttcac atcccgacca catacaggca 1620
aagtaatggc attaccgcga gccattactc ctacgcgcgc aattaacgaa tccaccatcg 1680
gggcagctgg tgtcgataac gaagtatctt caaccggttg agtattgagc gtatgttttg 1740
gaataacagg cgcacgcttc attatctaat ctcccagcgt ggtttaatca gacgatcgaa 1800
aatttcattg cagacaggtt cccaaataga aagagcattt ctccaggcac cagttgaaga 1860
gcgttgatca atggcctgtt caaaaacagt tctcatccgg atctgacctt taccaacttc 1920
atccgtttca cgtacaacat tttttagaac catgcttccc caggcatccc gaatttgctc 1980
ctccatccac ggggactgag agccattact attgctgtat ttggtaagca aaatacgtac 2040
atcaggctcg aaccctttaa gatcaacgtt cttgagcaga tcacgaagca tatcgaaaaa 2100
ctgcagtgcg gaggtgtagt caaacaactc agcaggcgtg ggaacaatca gcacatcagc 2160
agcacatacg acattaatcg tgccgatacc caggttaggc gcgctgtcaa taactatgac 2220
atcatagtca tgagcaacag tttcaatggc cagtcggagc atcaggtgtg gatcggtggg 2280
cagtttacct tcatcaaatt tgcccattaa ctcagtttca atacggtgca gagccagaca 2340
ggaaggaata atgtcaagcc ccggccagca agtgggcttt attgcataag tgacatcgtc 2400
cttttcccca agatagaaag gcaggagagt gtcttctgca tgaatatgaa gatctggtac 2460
ccatccgtga tacattgagg ctgttccctg ggggtcgtta ccttccacga gcaaaacacg 2520
tagccccttc agagccagat cctgagcaag atgaacagaa actgaggttt tgtaaacgcc 2580
acctttatgg gcagcaaccc cgatcaccgg tggaaatacg tcttcagcac gtcgcaatcg 2640
cgtaccaaac acatcacgca tatgattaat ttgttcaatt gtataaccaa cacgttgctc 2700
aacccgtcct cgaatttcca tatccgggtg cggtagtcgc cctgctttct cggcatctct 2760
gatagcctga gaagaaaccc caactaaatc cgctgcttca cctattctcc agcgccgggt 2820
tattttcctc gcttccgggc tgtcatcatt aaactgtgca atggcgatag ccttcgtcat 2880
ttcatgacca gcgtttatgc actggttaag tgtttccatg agtttcattc tgaacatcct 2940
ttaatcattg ctttgcgttt ttttattaaa tcttgcaatt tactgcaaag caacaacaaa 3000
atcgcaaagt catcaaaaaa ccgcaaagtt gtttaaaata agagcaacac tacaaaagga 3060
gataagaaga gcacatacct cagtcactta ttatcactag cgctcgccgc agccgtgtaa 3120
ccgagcatag cgagcgaact ggcgaggaag caaagaagaa ctgttctgtc agatagctct 3180
tacgctcagc gcaagaagaa atatccaccg tgggaaaaac tccaggtaga ggtacacacg 3240
cggatagcca attcagagta ataaactgtg ataatcaacc ctcatcaatg atgacgaact 3300
aacccccgat atcaggtcac atgacgaagg gaaagagaag gaaatcaact gtgacaaact 3360
gccctcaaat ttggcttcct taaaaattac agttcaaaaa gtatgagaaa atccatgcag 3420
gctgaaggaa acagcaaaac tgtgacaaat taccctcagt aggtcagaac aaatgtgacg 3480
aaccaccctc aaatctgtga cagataaccc tcagactatc ctgtcgtcat ggaagtgata 3540
tcgcggaagg aaaatacgat atgagtcgtc tggcggcctt tctttttctc aatgtatgag 3600
aggcgcattg gagttctgct gttgatctca ttaacacaga cctgcaggaa gcggcggcgg 3660
aagtcaggca tacgctggta actttgaggc agctggtaac gctctatgat ccagtcgatt 3720
ttcagagaga cgatgcctga gccatccggc ttacgatact gacacaggga ttcgtataaa 3780
cgcatggcat acggattggt gatttctttt gtttcactaa gccgaaactg cgtaaaccgg 3840
ttctgtaacc cgataaagaa gggaatgaga tatgggttga tatgtacact gtaaagccct 3900
ctggatggac tgtgcgcacg tttgataaac caaggaaaag attcatagcc tttttcatcg 3960
ccggcatcct cttcagggcg ataaaaaacc acttccttcc ccgcgaaact cttcaatgcc 4020
tgccgtatat ccttactggc ttccgcagag gtcaatccga atatttcagc atatttagca 4080
acatggatct cgcagatacc gtcatgttcc tgtagggtgc catcagattt tctgatctgg 4140
tcaacgaaca gatacagcat acgtttttga tcccgggaga gactatatgc cgcctcagtg 4200
aggtcgtttg actggacgat tcgcgggcta tttttacgtt tcttgtgatt gataaccgct 4260
gtttccgcca tgacagatcc atgtgaagtg tgacaagttt ttagattgtc acactaaata 4320
aaaaagagtc aataagcagg gataactttg tgaaaaaaca gcttcttctg agggcaattt 4380
gtcacagggt taagggcaat ttgtcacaga caggactgtc atttgagggt gatttgtcac 4440
actgaaaggg caatttgtca caacaccttc tctagaacca gcatggataa aggcctacaa 4500
ggcgctctaa aaaagaagat ctaaaaacta taaaaaaaat aattataaaa atatccccgt 4560
ggataagtgg ataaccccaa gggaagtttt ttcaggcatc gtgtgtaagc agaatatata 4620
agtgctgttc cctggtgctt cctcgctcac tcgagggctt cgccctgtcg ctcgactgcg 4680
gcgagcacta ctggctgtaa aaggacagac cacatcatgg ttctgtgttc attaggttgt 4740
tctgtccatt gctgacataa tccgctccac ttcaacgtaa caccgcacga agatttctat 4800
tgttcctgaa ggcatattca aatcgttttc gttaccgctt gcaggcatca tgacagaaca 4860
ctacttccta taaacgctac acaggctcct gagattaata atgcggatct ctacgataat 4920
gggagatttt cccgactgtt tcgttcgctt ctcagtggat aacagccagc ttctctgttt 4980
aacagacaaa aacagcatat ccactcagtt ccacatttcc atataaaggc caaggcattt 5040
attctcagga taattgtttc agcatcgcaa ccgcatcaga ctccggcatc gcaaactgca 5100
cccggtgccg ggcagccaca tccagcgcaa aaaccttcgt gtagacttcc gttgaactga 5160
tggacttatg tcccatcagg ctttgcagaa ctttcagcgg tataccggca tacagcatgt 5220
gcatcgcata ggaatggcgg aacgtatgtg gtgtgaccgg aacagagaac gtcacaccgt 5280
cagcagcagc ggcggcaacc gcctccccaa tccaggtcct gaccgttctg tccgtcactt 5340
cccagatccg cgctttctct gtccttcctg tgcgacggtt acgccgctcc atgagcttat 5400
cgcgaataaa tacctgtgac ggaagatcac ttcgcagaat aaataaatcc tggtgtccct 5460
gttgataccg ggaagccctg ggccaacttt tggcgaaaat gagacgttga tcggcacgta 5520
agaggttcca actttcacca taatgaaata agatcactac cgggcgtatt ttttgagtta 5580
tcgagatttt caggagctaa ggaagctaaa atggagaaaa aaatcactgg atataccacc 5640
gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc agttgctcaa 5700
tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac cgtaaagaaa 5760
aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat gaatgctcat 5820
ccggaattcc gtatggcaat gaaagacggt gagctggtga tatgggatag tgttcaccct 5880
tgttacaccg ttttccatga gcaaactgaa acgttttcat cgctctggag tgaataccac 5940
gacgatttcc ggcagtttct acacatatat tcgcaagatg tggcgtgtta cggtgaaaac 6000
ctggcctatt tccctaaagg gtttattgag aatatgtttt tcgtctcagc caatccctgg 6060
gtgagtttca ccagttttga tttaaacgtg gccaatatgg acaacttctt cgcccccgtt 6120
ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag 6180
gttcatcatg ccgtttgtga tggcttccat gtcggcagaa tgcttaatga attacaacag 6240
tactgcgatg agtggcaggg cggggcgtaa tttttttaag gcagttattg gtgcccttaa 6300
acgcctggtt gctacgcctg aataagtgat aataagcgga tgaatggcag aaattcgatg 6360
ataagctgtc aaacatgaga attggtcgac ttggcgcgcc taatacgact cactatagga 6420
ttaaaggttt ataccttccc aggtaacaaa ccaaccaact ttcgatctct tgtagatctg 6480
ttctctaaac gaactttaaa atctgtgtgg ctgtcactcg gctgcatgct tagtgcactc 6540
acgcagtata attaataact aattactgtc gttgacagga cacgagtaac tcgtctatct 6600
tctgcaggct gcttacggtt tcgtccgtgt tgcagccgat catcagcaca tctaggtttc 6660
gtccgggtgt gaccgaaagg taagatggag agccttgtcc ctggtttcaa cgagaaaaca 6720
cacgtccaac tcagtttgcc tgttttacag gttcgcgacg tgctcgtacg tggctttgga 6780
gactccgtgg aggaggtctt atcagaggca cgtcaacatc ttaaagatgg cacttgtggc 6840
ttagtagaag ttgaaaaagg cgttttgcct caacttgaac agccctatgt gttcatcaaa 6900
cgttcggatg ctcgaactgc acctcatggt catgttatgg ttgagctggt agcagaactc 6960
gaaggcattc agtacggtcg tagcggtgag acacttggtg tccttgtccc tcatgtgggc 7020
gaaataccag tggcttaccg caaggttctt cttcgtaaga acggtaataa aggagctggt 7080
ggccatagtt acggcgccga tctaaagtca tttgacttag gcgacgagct tggcactgat 7140
ccttatgaag attttcaaga aaactggaac actaaacata gcagtggtgt tacccgtgaa 7200
ctcatgcgtg agcttaacgg aggggcatac actcgctatg tcgataacaa cttctgtggc 7260
cctgatggct accctcttga gtgcattaaa gaccttctag cacgtgctgg taaagcttca 7320
tgcactttgt ccgaacaact ggactttatt gacactaaga ggggtgtata ctgctgccgt 7380
gaacatgagc atgaaattgc ttggtacacg gaacgttctg aaaagagcta tgaattgcag 7440
acaccttttg aaattaaatt ggcaaagaaa tttgacacct tcaatgggga atgtccaaat 7500
tttgtatttc ccttaaattc cataatcaag actattcaac caagggttga aaagaaaaag 7560
cttgatggct ttatgggtag aattcgatct gtctatccag ttgcgtcacc aaatgaatgc 7620
aaccaaatgt gcctttcaac tctcatgaag tgtgatcatt gtggtgaaac ttcatggcag 7680
acgggcgatt ttgttaaagc cacttgcgaa ttttgtggca ctgagaattt gactaaagaa 7740
ggtgccacta cttgtggtta cttaccccaa aatgctgttg ttaaaattta ttgtccagca 7800
tgtcacaatt cagaagtagg acctgagcat agtcttgccg aataccataa tgaatctggc 7860
ttgaaaacca ttcttcgtaa gggtggtcgc actattgcct ttggaggctg tgtgttctct 7920
tatgttggtt gccataacaa gtgtgcctat tgggttccac gtgctagcgc taacataggt 7980
tgtaaccata caggtgttgt tggagaaggt tccgaaggtc ttaatgacaa ccttcttgaa 8040
atactccaaa aagagaaagt caacatcaat attgttggtg actttaaact taatgaagag 8100
atcgccatta ttttggcatc tttttctgct tccacaagtg cttttgtgga aactgtgaaa 8160
ggtttggatt ataaagcatt caaacaaatt gttgaatcct gtggtaattt taaagttaca 8220
aaaggaaaag ctaaaaaagg tgcctggaat attggtgaac agaaatcaat actgagtcct 8280
ctttatgcat ttgcatcaga ggctgctcgt gttgtacgat caattttctc ccgcactctt 8340
gaaactgctc aaaattctgt gcgtgtttta cagaaggccg ctataacaat actagatgga 8400
atttcacagt attcactgag actcattgat gctatgatgt tcacatctga tttggctact 8460
aacaatctag ttgtaatggc ctacattaca ggtggtgttg ttcagttgac ttcgcagtgg 8520
ctaactaaca tctttggcac tgtttatgaa aaactcaaac ccgtccttga ttggcttgaa 8580
gagaagttta aggaaggtgt agagtttctt agagacggtt gggaaattgt taaatttatc 8640
tcaacctgtg cttgtgaaat tgtcggtgga caaattgtca cctgtgcaaa ggaaattaag 8700
gagagtgttc agacattctt taagcttgta aataaatttt tggctttgtg tgctgactct 8760
atcattattg gtggagctaa acttaaagcc ttgaatttag gtgaaacatt tgtcacgcac 8820
tcaaagggat tgtacagaaa gtgtgttaaa tccagagaag aaactggcct actcatgcct 8880
ctaaaagccc caaaagaaat tatcttctta gagggagaaa cacttcccac agaagtgtta 8940
acagaggaag ttgtcttgaa aactggtgat ttacaaccat tagaacaacc tactagtgaa 9000
gctgttgaag ctccattggt tggtacacca gtttgtatta acgggcttat gttgctcgaa 9060
atcaaagaca cagaaaagta ctgtgccctt gcacctaata tgatggtaac aaacaatacc 9120
ttcacactca aaggcggtgc accaacaaag gttacttttg gtgatgacac tgtgatagaa 9180
gtgcaaggtt acaagagtgt gaatatcact tttgaacttg atgaaaggat tgataaagta 9240
cttaatgaga agtgctctgc ctatacagtt gaactcggta cagaagtaaa tgagttcgcc 9300
tgtgttgtgg cagatgctgt cataaaaact ttgcaaccag tatctgaatt acttacacca 9360
ctgggcattg atttagatga gtggagtatg gctacatact acttatttga tgagtctggt 9420
gagtttaaat tggcttcaca tatgtattgt tctttctacc ctccagatga ggatgaagaa 9480
gaaggtgatt gtgaagaaga agagtttgag ccatcaactc aatatgagta tggtactgaa 9540
gatgattacc aaggtaaacc tttggaattt ggtgccactt ctgctgctct tcaacctgaa 9600
gaagagcaag aagaagattg gttagatgat gatagtcaac aaactgttgg tcaacaagac 9660
ggcagtgagg acaatcagac aactactatt caaacaattg ttgaggttca acctcaatta 9720
gagatggaac ttacaccagt tgttcagact attgaagtga atagttttag tggttattta 9780
aaacttactg acaatgtata cattaaaaat gcagacattg tggaagaagc taaaaaggta 9840
aaaccaacag tggttgttaa tgcagccaat gtttacctta aacatggagg aggtgttgca 9900
ggagccttaa ataaggctac taacaatgcc atgcaagttg aatctgatga ttacatagct 9960
actaatggac cacttaaagt gggtggtagt tgtgttttaa gcggacacaa tcttgctaaa 10020
cactgtcttc atgttgtcgg cctaaatgtt aacaaaggtg aagacattca acttcttaag 10080
agtgcttatg aaaattttaa tcagcacgaa gttctacttg caccattatt atcagctggt 10140
atttttggtg ctgaccctat acattcttta agagtttgtg tagatactgt tcgcacaaat 10200
gtctacttag ctgtctttga taaaaatctc tatgacaaac ttgtttcaag ctttttggaa 10260
atgaagagtg aaaagcaagt tgaacaaaag atcgctgaga ttcctaaaga ggaagttaag 10320
ccatttataa ctgaaagtaa accttcagtt gaacagagaa aacaagatga taagaaaatc 10380
aaagcttgtg ttgaagaagt tacaacaact ctggaagaaa ctaagttcct cacagaaaac 10440
ttgttacttt atattgacat taatggcaat cttcatccag attctgccac tcttgttagt 10500
gacattgaca tcactttctt aaagaaagat gctccatata tagtgggtga tgttattcaa 10560
gagggtgttt taactgctgt ggttatacct actaaaaagg ctggtggcac tactgaaatg 10620
ctagcgaaag ctttgagaaa agtgccaaca gacaattata taaccactta cccgggtcag 10680
ggtttaaatg gttacactgt agaggaggca aagacagtgc ttaaaaagtg taaaagtgcc 10740
ttttacattc taccatctat tatctctaat gagaagcaag aaattcttgg aactgtttct 10800
tggaatttgc gagaaatgct tgcacatgca gaagaaacac gcaaattaat gcctgtctgt 10860
gtggaaacta aagccatagt ttcaactata cagcgtaaat ataagggtat taaaatacaa 10920
gagggtgtgg ttgattatgg tgctagattt tacttttaca ccagtaaaac aactgtagcg 10980
tcacttatca acacacttaa cgatctaaat gaaactcttg ttacaatgcc acttggctat 11040
gtaacacatg gcttaaattt ggaagaagct gctcggtata tgagatctct caaagtgcca 11100
gctacagttt ctgtttcttc acctgatgct gttacagcgt ataatggtta tcttacttct 11160
tcttctaaaa cacctgaaga acattttatt gaaaccatct cacttgctgg ttcctataaa 11220
gattggtcct attctggaca atctacacaa ctaggtatag aatttcttaa gagaggtgat 11280
aaaagtgtat attacactag taatcctacc acattccacc tagatggtga agttatcacc 11340
tttgacaatc ttaagacact tctttctttg agagaagtga ggactattaa ggtgtttaca 11400
acagtagaca acattaacct ccacacgcaa gttgtggaca tgtcaatgac atatggacaa 11460
cagtttggtc caacttattt ggatggagct gatgttacta aaataaaacc tcataattca 11520
catgaaggta aaacatttta tgttttacct aatgatgaca ctctacgtgt tgaggctttt 11580
gagtactacc acacaactga tcctagtttt ctgggtaggt acatgtcagc attaaatcac 11640
actaaaaagt ggaaataccc acaagttaat ggtttaactt ctattaaatg ggcagataac 11700
aactgttatc ttgccactgc attgttaaca ctccaacaaa tagagttgaa gtttaatcca 11760
cctgctctac aagatgctta ttacagagca agggctggtg aagctgctaa cttttgtgca 11820
cttatcttag cctactgtaa taagacagta ggtgagttag gtgatgttag agaaacaatg 11880
agttacttgt ttcaacatgc caatttagat tcttgcaaaa gagtcttgaa cgtggtgtgt 11940
aaaacttgtg gacaacagca gacaaccctt aagggtgtag aagctgttat gtacatgggc 12000
acactttctt atgaacaatt taagaaaggt gttcagatac cttgtacgtg tggtaaacaa 12060
gctacaaaat atctagtaca acaggagtca ccttttgtta tgatgtcagc accacctgct 12120
cagtatgaac ttaagcatgg tacatttact tgtgctagtg agtacactgg taattaccag 12180
tgtggtcact ataaacatat aacttctaaa gaaactttgt attgcataga cggtgcttta 12240
cttacaaagt cctcagaata caaaggtcct attacggatg ttttctacaa agaaaacagt 12300
tacacaacaa ccataaaacc agttacttat aaattggatg gtgttgtttg tacagaaatt 12360
gaccctaagt tggacaatta ttataagaaa gacaattctt atttcacaga gcaaccaatt 12420
gatcttgtac caaaccaacc atatccaaac gcaagcttcg ataattttaa gtttgtatgt 12480
gataatatca aatttgctga tgatttaaac cagttaactg gttataagaa acctgcttca 12540
agagagctta aagttacatt tttccctgac ttaaatggtg atgtggtggc tattgattat 12600
aaacactaca caccctcttt taagaaagga gctaaattgt tacataaacc tattgtttgg 12660
catgttaaca atgcaactaa taaagccacg tataaaccaa atacctggtg tatacgttgt 12720
ctttggagca caaaaccagt tgaaacatca aattcgtttg atgtactgaa gtcagaggac 12780
gcgcagggaa tggataatct tgcctgcgaa gagctaaaac cagtctctga agaagtagtg 12840
gaaaatccta ccatacagaa agacgttctt gagtgtaatg tgaaaactac cgaagttgta 12900
ggagacatta tacttaaacc agcaaataat agtttaaaaa ttacagaaga ggttggccac 12960
acagatctaa tggctgctta tgtagacaat tctagtctta ctattaagaa acctaatgaa 13020
ttatctagag tattaggttt gaaaaccctt gctactcatg gtttagctgc tgttaatagt 13080
gtcccttggg atactatagc taattatgct aagccttttc ttaacaaagt tgttagtaca 13140
actactaaca tagttacacg gtgtttaaac cgtgtttgta ctaattatat gccttatttc 13200
tttactttat tgctacaatt gtgtactttt actagaagta caaattctag aattaaagca 13260
tctatgccga ctactatagc aaagaatact gttaagagtg tcggtaaatt ttgtctagag 13320
gcttcattta attatttgaa gtcacctaat ttttctaaac tgataaatat tataatttgg 13380
tttttactat taagtgtttg cctaggttct ttaatctact caaccgctgc tttaggtgtt 13440
ttaatgtcta atttaggcat gccttcttac tgtactggtt acagagaagg ctatttgaac 13500
tctactaatg tcactattgc aacctactgt actggttcta taccttgtag tgtttgtctt 13560
agtggtttag attctttaga cacctatcct tctttagaaa ctatacaaat taccatttca 13620
tcttttaaat gggatttaac tgcttttggc ttagttgcag agtggttttt ggcatatatt 13680
cttttcacta ggtttttcta tgtacttgga ttggctgcaa tcatgcaatt gtttttcagc 13740
tattttgcag tacattttat tagtaattct tggcttatgt ggttaataat taatcttgta 13800
caaatggccc cgatttcagc tatggttaga atgtacatct tctttgcatc attttattat 13860
gtatggaaaa gttatgtgca tgttgtagac ggttgtaatt catcaacttg tatgatgtgt 13920
tacaaacgta atagagcaac aagagtcgaa tgtacaacta ttgttaatgg tgttagaagg 13980
tccttttatg tctatgctaa tggaggtaaa ggcttttgca aactacacaa ttggaattgt 14040
gttaattgtg atacattctg tgctggtagt acatttatta gtgatgaagt tgcgagagac 14100
ttgtcactac agtttaaaag accaataaat cctactgacc agtcttctta catcgttgat 14160
agtgttacag tgaagaatgg ttccatccat ctttactttg ataaagctgg tcaaaagact 14220
tatgaaagac attctctctc tcattttgtt aacttagaca acctgagagc taataacact 14280
aaaggttcat tgcctattaa tgttatagtt tttgatggta aatcaaaatg tgaagaatca 14340
tctgcaaaat cagcgtctgt ttactacagt cagcttatgt gtcaacctat actgttacta 14400
gatcaggcat tagtgtctga tgttggtgat agtgcggaag ttgcagttaa aatgtttgat 14460
gcttacgtta atacgttttc atcaactttt aacgtaccaa tggaaaaact caaaacacta 14520
gttgcaactg cagaagctga acttgcaaag aatgtgtcct tagacaatgt cttatctact 14580
tttatttcag cagctcggca agggtttgtt gattcagatg tagaaactaa agatgttgtt 14640
gaatgtctta aattgtcaca tcaatctgac atagaagtta ctggcgatag ttgtaataac 14700
tatatgctca cctataacaa agttgaaaac atgacacccc gtgaccttgg tgcttgtatt 14760
gactgtagtg cgcgtcatat taatgcgcag gtagcaaaaa gtcacaacat tgctttgata 14820
tggaacgtta aagatttcat gtcattgtct gaacaactac gaaaacaaat acgtagtgct 14880
gctaaaaaga ataacttacc ttttaagttg acatgtgcaa ctactagaca agttgttaat 14940
gttgtaacaa caaagatagc acttaagggt ggtaaaattg ttaataattg gttgaagcag 15000
ttaattaaag ttacacttgt gttccttttt gttgctgcta ttttctattt aataacacct 15060
gttcatgtca tgtctaaaca tactgacttt tcaagtgaaa tcataggata caaggctatt 15120
gatggtggtg tcactcgtga catagcatct acagatactt gttttgctaa caaacatgct 15180
gattttgaca catggtttag ccagcgtggc ggtagttata ctaatgacaa agcttgccca 15240
ttgattgctg cagtcataac aagagaagtg ggttttgtcg tgcctggttt gcctggcacg 15300
atattacgca caactaatgg tgactttttg catttcttac ctagagtttt tagtgcagtt 15360
ggtaacatct gttacacacc atcaaaactt atagagtaca ctgactttgc aacatcagct 15420
tgtgttttgg ctgctgaatg tacaattttt aaagatgctt ctggtaagcc agtaccatat 15480
tgttatgata ccaatgtact agaaggttct gttgcttatg aaagtttacg ccctgacaca 15540
cgttatgtgc tcatggatgg ctctattatt caatttccta acacctacct tgaaggttct 15600
gttagagtgg taacaacttt tgattctgag tactgtaggc acggcacttg tgaaagatca 15660
gaagctggtg tttgtgtatc tactagtggt agatgggtac ttaacaatga ttattacaga 15720
tctttaccag gagttttctg tggtgtagat gctgtaaatt tacttactaa tatgtttaca 15780
ccactaattc aacctattgg tgctttggac atatcagcat ctatagtagc tggtggtatt 15840
gtagctatcg tagtaacatg ccttgcctac tattttatga ggtttagaag agcttttggt 15900
gaatacagtc atgtagttgc ctttaatact ttactattcc ttatgtcatt cactgtactc 15960
tgtttaacac cagtttactc attcttacct ggtgtttatt ctgttattta cttgtacttg 16020
acattttatc ttactaatga tgtttctttt ttagcacata ttcagtggat ggttatgttc 16080
acacctttag tacctttctg gataacaatt gcttatatca tttgtatttc cacaaagcat 16140
ttctattggt tctttagtaa ttacctaaag agacgtgtag tctttaatgg tgtttccttt 16200
agtacttttg aagaagctgc gctgtgcacc tttttgttaa ataaagaaat gtatctaaag 16260
ttgcgtagtg atgtgctatt acctcttacg caatataata gatacttagc tctttataat 16320
aagtacaagt attttagtgg agcaatggat acaactagct acagagaagc tgcttgttgt 16380
catctcgcaa aggctctcaa tgacttcagt aactcaggtt ctgatgttct ttaccaacca 16440
ccacaaacct ctatcacctc agctgttttg cagagtggtt ttagaaaaat ggcattccca 16500
tctggtaaag ttgagggttg tatggtacaa gtaacttgtg gtacaactac acttaacggt 16560
ctttggcttg atgacgtagt ttactgtcca agacatgtga tctgcacctc tgaagacatg 16620
cttaacccta attatgaaga tttactcatt cgtaagtcta atcataattt cttggtacag 16680
gctggtaatg ttcaactcag ggttattgga cattctatgc aaaattgtgt acttaagctt 16740
aaggttgata cagccaatcc taagacacct aagtataagt ttgttcgcat tcaaccagga 16800
cagacttttt cagtgttagc ttgttacaat ggttcaccat ctggtgttta ccaatgtgct 16860
atgaggccca atttcactat taagggttca ttccttaatg gttcatgtgg tagtgttggt 16920
tttaacatag attatgactg tgtctctttt tgttacatgc accatatgga attaccaact 16980
ggagttcatg ctggcacaga cttagaaggt aacttttatg gaccttttgt tgacaggcaa 17040
acagcacaag cagctggtac ggacacaact attacagtta atgttttagc ttggttgtac 17100
gctgctgtta taaatggaga caggtggttt ctcaatcgat ttaccacaac tcttaatgac 17160
tttaaccttg tggctatgaa gtacaattat gaacctctaa cacaagacca tgttgacata 17220
ctaggacctc tttctgctca aactggaatt gccgttttag atatgtgtgc ttcattaaaa 17280
gaattactgc aaaatggtat gaatggacgt accatattgg gtagtgcttt attagaagat 17340
gaatttacac cttttgatgt tgttagacaa tgctcaggtg ttactttcca aagtgcagtg 17400
aaaagaacaa tcaagggtac acaccactgg ttgttactca caattttgac ttcactttta 17460
gttttagtcc agagtactca atggtctttg ttcttttttt tgtatgaaaa tgccttttta 17520
ccttttgcta tgggtattat tgctatgtct gcttttgcaa tgatgtttgt caaacataag 17580
catgcatttc tctgtttgtt tttgttacct tctcttgcca ctgtagctta ttttaatatg 17640
gtctatatgc ctgctagttg ggtgatgcgt attatgacat ggttggatat ggttgatact 17700
agtttgtctg gttttaagct aaaagactgt gttatgtatg catcagctgt agtgttacta 17760
atccttatga cagcaagaac tgtgtatgat gatggtgcta ggagagtgtg gacacttatg 17820
aatgtcttga cactcgttta taaagtttat tatggtaatg ctttagatca agccatttcc 17880
atgtgggctc ttataatctc tgttacttct aactactcag gtgtagttac aactgtcatg 17940
tttttggcca gaggtattgt ttttatgtgt gttgagtatt gccctatttt cttcataact 18000
ggtaatacac ttcagtgtat aatgctagtt tattgtttct taggctattt ttgtacttgt 18060
tactttggcc tcttttgttt actcaaccgc tactttagac tgactcttgg tgtttatgat 18120
tacttagttt ctacacagga gtttagatat atgaattcac agggactact cccacccaag 18180
aatagcatag atgccttcaa actcaacatt aaattgttgg gtgttggtgg caaaccttgt 18240
atcaaagtag ccactgtaca gtctaaaatg tcagatgtaa agtgcacgtc agtagtctta 18300
ctctcagttt tgcaacaact cagagtagaa tcatcatcta aattgtgggc tcaatgtgtc 18360
cagttacaca atgacattct cttagctaaa gatactactg aagcctttga aaaaatggtt 18420
tcactacttt ctgttttgct ttccatgcag ggtgctgtag acataaacaa gctttgtgaa 18480
gaaatgctgg acaacagggc aaccttacaa gctatagcct cagagtttag ttcccttcca 18540
tcatatgcag cttttgctac tgctcaagaa gcttatgagc aggctgttgc taatggtgat 18600
tctgaagttg ttcttaaaaa gttgaagaag tctttgaatg tggctaaatc tgaatttgac 18660
cgtgatgcag ccatgcaacg taagttggaa aagatggctg atcaagctat gacccaaatg 18720
tataaacagg ctagatctga ggacaagagg gcaaaagtta ctagtgctat gcagacaatg 18780
cttttcacta tgcttagaaa gttggataat gatgcactca acaacattat caacaatgca 18840
agagatggtt gtgttccctt gaacataata cctcttacaa cagcagccaa actaatggtt 18900
gtcataccag actataacac atataaaaat acgtgtgatg gtacaacatt tacttatgca 18960
tcagcattgt gggaaatcca acaggttgta gatgcagata gtaaaattgt tcaacttagt 19020
gaaattagta tggacaattc acctaattta gcatggcctc ttattgtaac agccttaagg 19080
gccaattctg ctgtcaaatt acagaataat gagcttagtc ctgttgcact acgacagatg 19140
tcttgtgctg ccggtactac acaaactgct tgcactgatg acaatgcgtt agcttactac 19200
aacacaacaa agggaggtag gtttgtactt gcactgttat ccgatttaca ggatttgaaa 19260
tgggctagat tccctaagag tgatggaact ggtactatct atacagaact ggaaccacct 19320
tgtaggtttg ttacagacac acctaaaggt cctaaagtga agtatttata ctttattaaa 19380
ggattaaaca acctaaatag aggtatggta cttggtagtt tagctgccac agtacgtcta 19440
caagctggta atgcaacaga agtgcctgcc aattcaactg tattatcttt ctgtgctttt 19500
gctgtagatg ctgctaaagc ttacaaagat tatctagcta gtgggggaca accaatcact 19560
aattgtgtta agatgttgtg tacatacact ggtactggtc aggcaataac agttacaccg 19620
gaagccaata tggatcaaga atcctttggt ggtgcatcgt gttgtctgta ctgccgttgc 19680
cacatagatc atccaaatcc taaaggattt tgtgacttaa aaggtaagta tgtacaaata 19740
cctacaactt gtgctaatga ccctgtgggt tttacactta aaaacacagt ctgtaccgtc 19800
tgcggtatgt ggaaaggtta tggctgtagt tgtgatcaac tccgcgaacc catgcttcag 19860
tcagctgatg cacaatcgtt tttaaacggg tttgcggtgt aagtgcagcc cgtcttacac 19920
cgtgcggcac aggcactagt actgatgtcg tatacagggc ttttgacatc tacaatgata 19980
aagtagctgg ttttgctaaa ttcctaaaaa ctaattgttg tcgcttccaa gaaaaggacg 20040
aagatgacaa tttaattgat tcttactttg tagttaagag acacactttc tctaactacc 20100
aacatgaaga aacaatttat aatttactta aggattgtcc agctgttgct aaacatgact 20160
tctttaagtt tagaatagac ggtgacatgg taccacatat atcacgtcaa cgtcttacta 20220
aatacacaat ggcagacctc gtctatgctt taaggcattt tgatgaaggt aattgtgaca 20280
cattaaaaga aatacttgtc acatacaatt gttgtgatga tgattatttc aataaaaagg 20340
actggtatga ttttgtagaa aacccagata tattacgcgt atacgccaac ttaggtgaac 20400
gtgtacgcca agctttgtta aaaacagtac aattctgtga tgccatgcga aatgctggta 20460
ttgttggtgt actgacatta gataatcaag atctcaatgg taactggtat gatttcggtg 20520
atttcataca aaccacgcca ggtagtggag ttcctgttgt agattcttat tattcattgt 20580
taatgcctat attaaccttg accagggctt taactgcaga gtcacatgtt gacactgact 20640
taacaaagcc ttacattaag tgggatttgt taaaatatga cttcacggaa gagaggttaa 20700
aactctttga ccgttatttt aaatattggg atcagacata ccacccaaat tgtgttaact 20760
gtttggatga cagatgcatt ctgcattgtg caaactttaa tgttttattc tctacagtgt 20820
tcccacctac aagttttgga ccactagtga gaaaaatatt tgttgatggt gttccatttg 20880
tagtttcaac tggataccac ttcagagagc taggtgttgt acataatcag gatgtaaact 20940
tacatagctc tagacttagt tttaaggaat tacttgtgta tgctgctgac cctgctatgc 21000
acgctgcttc tggtaatcta ttactagata aacgcactac gtgcttttca gtagctgcac 21060
ttactaacaa tgttgctttt caaactgtca aacccggtaa ttttaacaaa gacttctatg 21120
actttgctgt gtctaagggt ttctttaagg aaggaagttc tgttgaatta aaacacttct 21180
tctttgctca ggatggtaat gctgctatca gcgattatga ctactatcgt tataatctac 21240
caacaatgtg tgatatcaga caactactat ttgtagttga agttgttgat aagtactttg 21300
attgttacga tggtggctgt attaatgcta accaagtcat cgtcaacaac ctagacaaat 21360
cagctggttt tccatctaat aaatggggta aggctagact ttattatgat tcaatgagtt 21420
atgaggatca agatgcactt ttcgcatata caaaacgtaa tgtcatccct actataactc 21480
aaatgaatct taagtatgcc attagtgcaa agaatagagc tcgcaccgta gctggtgtct 21540
ctatctgtag tactatgacc aatagacagt ttcatcaaaa attattgaaa tcaatagccg 21600
ccactagagg agctactgta gtaattggaa caagcaaatt ctatggtggt tggcacaaca 21660
tgttaaaaac tgtttatagt gatgtagaaa accctcacct tatgggttgg gattatccta 21720
aatgtgatag agccatgcct aacatgctta gaattatggc ctcacttgtt cttgctcgca 21780
aacatacaac gtgttgtagc ttgtcacacc gtttctatag attagctaat gagtgtgctc 21840
aagtattgag tgaaatggtc atgtgtggcg gttcactata tgttaaacca ggtggaacct 21900
catcaggaga tgccacaact gcttatgcta atagtgtttt taacatttgt caagctgtca 21960
cggccaatgt taatgcactt ttatctactg atggtaacaa aattgccgat aagtatgtcc 22020
gcaatttaca acacagactt tatgagtgtc tctatagaaa tagagatgtt gacacagact 22080
ttgtgaatga gttttacgca tatttgcgta aacatttctc aatgatgata ctctctgacg 22140
atgctgttgt gtgtttcaat agcacttatg catctcaagg tctagtggct agcataaaga 22200
actttaagtc agttctttat tatcaaaaca atgtttttat gtctgaagca aaatgttgga 22260
ctgagactga ccttactaaa ggacctcatg aattttgctc tcaacataca atgctagtta 22320
aacagggtga tgattatgtg taccttcctt acccagatcc atcaagaatc ctaggggccg 22380
gctgttttgt agatgatatc gtaaaaacag atggtacact tatgattgaa cggttcgtgt 22440
ctttagctat agatgcttac ccacttacta aacatcctaa tcaggagtat gctgatgtct 22500
ttcatttgta cttacaatac ataagaaagc tacatgatga gttaacagga cacatgttag 22560
acatgtattc tgttatgctt actaatgata acacttcaag gtattgggaa cctgagtttt 22620
atgaggctat gtacacaccg catacagtct tacaggctgt tggggcttgt gttctttgca 22680
attcacagac ttcattaaga tgtggtgctt gcatacgtag accattctta tgttgtaaat 22740
gctgttacga ccatgtcata tcaacatcac ataaattagt cttgtctgtt aatccgtatg 22800
tttgcaatgc tccaggttgt gatgtcacag atgtgactca actttactta ggaggtatga 22860
gctattattg taaatcacat aaaccaccca ttagttttcc attgtgtgct aatggacaag 22920
tttttggttt atataaaaat acatgtgttg gtagcgataa tgttactgac tttaatgcaa 22980
ttgcaacatg tgactggaca aatgctggtg attacatttt agctaacacc tgtactgaaa 23040
gactcaagct ttttgcagca gaaacgctca aagctactga ggagacattt aaactgtctt 23100
atggtattgc tactgtacgt gaagtgctgt ctgacagaga attacatctt tcatgggaag 23160
ttggtaaacc tagaccacca cttaaccgaa attatgtctt tactggttat cgtgtaacta 23220
aaaacagtaa agtacaaata ggagagtaca cctttgaaaa aggtgactat ggtgatgctg 23280
ttgtttaccg aggtacaaca acttacaaat taaatgttgg tgattatttt gtgctgacat 23340
cacatacagt aatgccatta agtgcaccta cactagtgcc acaagagcac tatgttagaa 23400
ttactggctt atacccaaca ctcaatatct cagatgagtt ttctagcaat gttgcaaatt 23460
atcaaaaggt tggtatgcaa aagtattcta cactccaggg accacctggt actggtaaga 23520
gtcattttgc tattggccta gctctctact acccttctgc tcgcatagtg tatacagctt 23580
gctctcatgc cgctgttgat gcactatgtg agaaggcatt aaaatatttg cctatagata 23640
aatgtagtag aattatacct gcacgtgctc gtgtagagtg ttttgataaa ttcaaagtga 23700
attcaacatt agaacagtat gtcttttgta ctgtaaatgt attgcctgag acgacagcag 23760
atatagttgt ctttgatgaa atttcaatgg ccacaaatta tgatttgagt gttgtcaatg 23820
ccagattacg tgctaagcac tatgtgtaca ttggcgaccc tgctcaatta cctgcaccac 23880
gcacattgct aactaagggc acactagaac cagaatattt caattcagtg tgtagactta 23940
tgaaaactat aggtccagac atgttcctcg gaacttgtcg gcgttgtcct gctgaaattg 24000
ttgacactgt gagtgctttg gtttatgata ataagcttaa agcacataaa gacaaatcag 24060
ctcaatgctt taaaatgttt tataagggtg ttatcacgca tgatgtttca tctgcaatta 24120
acaggccaca aataggcgtg gtaagagaat tccttacacg taaccctgct tggagaaaag 24180
ctgtctttat ttcaccttat aattcacaga atgctgtagc ctcaaagatt ttgggactac 24240
caattcaaac tgttgattca tcacagggct cagaatatga ctatgtcata ttcactcaaa 24300
ccactgaaac agctcactct tgtaatgtaa acagatttaa tgttgctatt accagagcaa 24360
aagtaggcat actttgcata atgtctgata gagaccttta tgacaagttg caatttacaa 24420
gtcttgaaat tccacgtagg aatgtggcaa ctttacaagc tgaaaatgta acaggactct 24480
ttaaagattg tagtaaggta atcactgggt tacatcctac acaggcacct acacacctca 24540
gtgttgacac taaattcaaa actgaaggtt tatgtgttga catacctggc atacctaagg 24600
acatgaccta tagaagactc atctctatga tgggttttaa aatgaattat caagttaatg 24660
gttaccctaa catgtttatc acccgcgaag aagctataag acatgtacgt gcatggattg 24720
gcttcgatgt cgaggggtgt catgctacta gagaagctgt tggtaccaat ttacctttac 24780
agctaggttt ttctacaggt gttaacctag ttgctgtacc tacaggttat gttgatacac 24840
ctaataatac agatttctcc agagttagtg ctaaaccacc gcctggagat caatttaaac 24900
acctcatacc acttatgtac aaaggacttc cttggaatgt agtgcgtata aagattgtac 24960
aaatgttaag tgacacactt aaaaatctct ctgacagagt cgtatttgtc ttatgggcac 25020
atggctttga gttgacatct atgaagtatt ttgtgaaaat aggacctgag cgcacctgtt 25080
gtctatgtga tagacgtgcc acatgctttt ccactgcttc agacacttat gcctgttggc 25140
atcattctat tggatttgat tacgtctata atccgtttat gattgatgtt caacaatggg 25200
gttttacagg taacctacaa agcaaccatg atctgtattg tcaagtccat ggtaatgcac 25260
atatagctag ttgtgatgca atcatgacta ggtgtctagc tgtccacgag tgctttgtta 25320
agcgtgttga ctggactatt gaatatccta taattggtga tgaactgaag attaatgcgg 25380
cttgtagaaa ggttcaacac atggttgtta aagctgcatt attagcagac aaattcccag 25440
ttcttcacga cattggtaac cctaaagcta ttaagtgtgt acctcaagct gatgtagaat 25500
ggaagttcta tgatgcacag ccttgtagtg acaaagctta taaaatagaa gaattattct 25560
attcttatgc cacacattct gacaaattca cagatggtgt atgcctattt tggaattgca 25620
atgtcgatag atatcctgct aattccattg tttgtagatt tgacactaga gtgctatcta 25680
accttaactt gcctggttgt gatggtggca gtttgtatgt aaataaacat gcattccaca 25740
caccagcttt tgataaaagt gcttttgtta atttaaaaca attaccattt ttctattact 25800
ctgacagtcc atgtgagtct catggaaaac aagtagtgtc agatatagat tatgtaccac 25860
taaagtctgc tacgtgtata acacgttgca atttaggtgg tgctgtctgt agacatcatg 25920
ctaatgagta cagattgtat ctcgatgctt ataacatgat gatctcagct ggctttagct 25980
tgtgggttta caaacaattt gatacttata acctctggaa cacttttaca agacttcaga 26040
gtttagaaaa tgtggctttt aatgttgtaa ataagggaca ctttgatgga caacagggtg 26100
aagtaccagt ttctatcatt aataacactg tttacacaaa agttgatggt gttgatgtag 26160
aattgtttga aaataaaaca acattacctg ttaatgtagc atttgagctt tgggctaagc 26220
gcaacattaa accagtacca gaggtgaaaa tactcaataa tttgggtgtg gacattgctg 26280
ctaatactgt gatctgggac tacaaaagag atgctccagc acatatatct actattggtg 26340
tttgttctat gactgacata gccaagaaac caactgaaac gatttgtgca ccactcactg 26400
tcttttttga tggtagagtt gatggtcaag tagacttatt tagaaatgcc cgtaatggtg 26460
ttcttattac agaaggtagt gttaaaggtt tacaaccatc tgtaggtccc aaacaagcta 26520
gtcttaatgg agtcacatta attggagaag ccgtaaaaac acagttcaat tattataaga 26580
aagttgatgg tgttgtccaa caattacctg aaacttactt tactcagagt agaaatttac 26640
aagaatttaa acccaggagt caaatggaaa ttgatttctt agaattagct atggatgaat 26700
tcattgaacg gtataaatta gaaggctatg ccttcgaaca tatcgtttat ggagatttta 26760
gtcatagtca gttaggtggt ttacatctac tgattggact agctaaacgt tttaaggaat 26820
caccttttga attagaagat tttattccta tggacagtac agttaaaaac tatttcataa 26880
cagatgcgca aacaggttca tctaagtgtg tgtgttctgt tattgattta ttacttgatg 26940
attttgttga aataataaaa tcccaagatt tatctgtagt ttctaaggtt gtcaaagtga 27000
ctattgacta tacagaaatt tcatttatgc tttggtgtaa agatggccat gtagaaacat 27060
tttacccaaa attacaatct agtcaagcgt ggcaaccggg tgttgctatg cctaatcttt 27120
acaaaatgca aagaatgcta ttagaaaagt gtgaccttca aaattatggt gatagtgcaa 27180
cattacctaa aggcataatg atgaatgtcg caaaatatac tcaactgtgt caatatttaa 27240
acacattaac attagctgta ccctataata tgagagttat acattttggt gctggttctg 27300
ataaaggagt tgcaccaggt acagctgttt taagacagtg gttgcctacg ggtacgctgc 27360
ttgtcgattc agatcttaat gactttgtct ctgatgcaga ttcaactttg attggtgatt 27420
gtgcaactgt acatacagct aataaatggg atctcattat tagtgatatg tacgacccta 27480
agactaaaaa tgttacaaaa gaaaatgact ctaaagaggg ttttttcact tacatttgtg 27540
ggtttataca acaaaagcta gctcttggag gttccgtggc tataaagata acagaacatt 27600
cttggaatgc tgatctttat aagctcatgg gacacttcgc atggtggaca gcctttgtta 27660
ctaatgtgaa tgcgtcatca tctgaagcat ttttaattgg atgtaattat cttggcaaac 27720
cacgcgaaca aatagatggt tatgtcatgc atgcaaatta catattttgg aggaatacaa 27780
atccaattca gttgtcttcc tattctttat ttgacatgag taaatttccc cttaaattaa 27840
ggggtactgc tgttatgtct ttaaaagaag gtcaaatcaa tgatatgatt ttatctcttc 27900
ttagtaaagg tagacttata attagagaaa acaacagagt tgttatttct agtgatgttc 27960
ttgttaacaa ctaaacgaac aatgtttgtt tttcttgttt tattgccact agtctctagt 28020
cagtgtgtta atcttacaac cagaactcaa ttaccccctg catacactaa ttctttcaca 28080
cgtggtgttt attaccctga caaagttttc agatcctcag ttttacattc aactcaggac 28140
ttgttcttac ctttcttttc caatgttact tggttccatg ctatacatgt ctctgggacc 28200
aaaggtacta agaggtttga taaccctgtc ctaccattta atgatggtgt ttattttgct 28260
tccactgaga agtctaacat aataagaggc tggatttttg gtactacttt agattcgaag 28320
acccagtccc tacttattgt taataacgct actaatgttg ttattaaagt ctgtgaattt 28380
caattttgta atgatccatt tttgggtgtt tattaccaca aaaacaacaa aagttggatg 28440
gaaagtgagt tcagagttta ttctagtgcg aataattgca cttttgaata tgtctctcag 28500
ccttttctta tggaccttga aggaaaacag ggtaatttca aaaatcttag ggaatttgtg 28560
tttaagaata ttgatggtta ttttaaaata tattctaagc acacgcctat taatttagtg 28620
cgtgatctcc ctcagggttt ttcggcttta gaaccattgg tagatttgcc aataggtatt 28680
aacatcacta ggtttcaaac tttacttgct ttacatagaa gttatttgac tcctggtgat 28740
tcttcttcag gttggacagc tggtgctgca gcttattatg tgggttatct tcaacctagg 28800
acttttctat taaaatataa tgaaaatgga accattacag atgctgtaga ctgtgcactt 28860
gaccctctct cagaaacaaa gtgtacgttg aaatccttca ctgtagaaaa aggaatctat 28920
caaacttcta actttagagt ccaaccaaca gaatctattg ttagatttcc taatattaca 28980
aacttgtgcc cttttggtga agtttttaac gccaccagat ttgcatctgt ttatgcttgg 29040
aacaggaaga gaatcagcaa ctgtgttgct gattattctg tcctatataa ttccgcatca 29100
ttttccactt ttaagtgtta tggagtgtct cctactaaat taaatgatct ctgctttact 29160
aatgtctatg cagattcatt tgtaattaga ggtgatgaag tcagacaaat cgctccaggg 29220
caaactggaa agattgctga ttataattat aaattaccag atgattttac aggctgcgtt 29280
atagcttgga attctaacaa tcttgattct aaggttggtg gtaattataa ttacctgtat 29340
agattgttta ggaagtctaa tctcaaacct tttgagagag atatttcaac tgaaatctat 29400
caggccggta gcacaccttg taatggtgtt gaaggtttta attgttactt tcctttacaa 29460
tcatatggtt tccaacccac taatggtgtt ggttaccaac catacagagt agtagtactt 29520
tcttttgaac ttctacatgc accagcaact gtttgtggac ctaaaaagtc tactaatttg 29580
gttaaaaaca aatgtgtcaa tttcaacttc aatggtttaa caggcacagg tgttcttact 29640
gagtctaaca aaaagtttct gcctttccaa caatttggca gagacattgc tgacactact 29700
gatgctgtcc gtgatccaca gacacttgag attcttgaca ttacaccatg ttcttttggt 29760
ggtgtcagtg ttataacacc aggaacaaat acttctaacc aggttgctgt tctttatcag 29820
gatgttaact gcacagaagt ccctgttgct attcatgcag atcaacttac tcctacttgg 29880
cgtgtttatt ctacaggttc taatgttttt caaacacgtg caggctgttt aataggggct 29940
gaatatgtca acaactcata tgagtgtgac atacccattg gtgcaggtat atgcgctagt 30000
tatcagactc agactaattc tcctcggcgg gcacgtagtg tagctagtca atccatcatt 30060
gcctacacta tgtcacttgg tgcagaaaat tcagttgctt actctaataa ctctattgcc 30120
atacccacaa attttactat tagtgttacc acagaaattc taccagtgtc tatgaccaag 30180
acatcagtag attgtacaat gtacatttgt ggtgattcaa ctgaatgcag caatcttttg 30240
ttgcaatatg gcagtttttg tacacaatta aaccgtgctt taactggaat agctgttgaa 30300
caagacaaaa acacccaaga agtttttgca caagtcaaac aaatttacaa aacaccacca 30360
attaaagatt ttggtggttt taatttttca caaatattac cagatccatc aaaaccaagc 30420
aagaggtcat ttattgaaga tctacttttc aacaaagtga cacttgcaga tgctggcttc 30480
atcaaacaat atggtgattg ccttggtgat attgctgcta gagacctcat ttgtgcacaa 30540
aagtttaacg gccttactgt tttgccacct ttgctcacag atgaaatgat tgctcaatac 30600
acttctgcac tgttagcggg tacaatcact tctggttgga cctttggtgc aggtgctgca 30660
ttacaaatac catttgctat gcaaatggct tataggttta atggtattgg agttacacag 30720
aatgttctct atgagaacca aaaattgatt gccaaccaat ttaatagtgc tattggcaaa 30780
attcaagact cactttcttc cacagcaagt gcacttggaa aacttcaaga tgtggtcaac 30840
caaaatgcac aagctttaaa cacgcttgtt aaacaactta gctccaattt tggtgcaatt 30900
tcaagtgttt taaatgatat cctttcacgt cttgacaaag ttgaggctga agtgcaaatt 30960
gataggttga tcacaggcag acttcaaagt ttgcagacat atgtgactca acaattaatt 31020
agagctgcag aaatcagagc ttctgctaat cttgctgcta ctaaaatgtc agagtgtgta 31080
cttggacaat caaaaagagt tgatttttgt ggaaagggct atcatcttat gtccttccct 31140
cagtcagcac ctcatggtgt agtcttcttg catgtgactt atgtccctgc acaagaaaag 31200
aacttcacaa ctgctcctgc catttgtcat gatggaaaag cacactttcc tcgtgaaggt 31260
gtctttgttt caaatggcac acactggttt gtaacacaaa ggaattttta tgaaccacaa 31320
atcattacta cagacaacac atttgtgtct ggtaactgtg atgttgtaat aggaattgtc 31380
aacaacacag tttatgatcc tttgcaacct gaattagact cattcaagga ggagttagat 31440
aaatatttta agaatcatac atcaccagat gttgatttag gtgacatctc tggcattaat 31500
gcttcagttg taaacattca aaaagaaatt gaccgcctca atgaggttgc caagaattta 31560
aatgaatctc tcatcgatct ccaagaactt ggaaagtatg agcagtatat aaaatggcca 31620
tggtacattt ggctaggttt tatagctggc ttgattgcca tagtaatggt gacaattatg 31680
ctttgctgta tgaccagttg ctgtagttgt ctcaagggct gttgttcttg tggatcctgc 31740
tgcaaatttg atgaagacga ctctgagcca gtgctcaaag gagtcaaatt acattacaca 31800
taaacgaact tatggatttg tttatgagaa tcttcacaat tggaactgta actttgaagc 31860
aaggtgaaat caaggatgct actccttcag attttgttcg cgctactgca acgataccga 31920
tacaagcctc actccctttc ggatggctta ttgttggcgt tgcacttctt gctgtttttc 31980
agagcgcttc caaaatcata accctcaaaa agagatggca actagcactc tccaagggtg 32040
ttcactttgt ttgcaacttg ctgttgttgt ttgtaacagt ttactcacac cttttgctcg 32100
ttgctgctgg ccttgaagcc ccttttctct atctttatgc tttagtctac ttcttgcaga 32160
gtataaactt tgtaagaata ataatgaggc tttggctttg ctggaaatgc cgttccaaaa 32220
acccattact ttatgatgcc aactattttc tttgctggca tactaattgt tacgactatt 32280
gtatacctta caatagtgta acttcttcaa ttgtcattac ttcaggtgat ggcacaacaa 32340
gtcctatttc tgaacatgac taccagattg gtggttatac tgaaaaatgg gaatctggag 32400
taaaagactg tgttgtatta cacagttact tcacttcaga ctattaccag ctgtactcaa 32460
ctcaattgag tacagacact ggtgttgaac atgttacctt cttcatctac aataaaattg 32520
ttgatgagcc tgaagaacat gtccaaattc acacaatcga cggttcatcc ggagttgtta 32580
atccagtaat ggaaccaatt tatgatgaac cgacgacgac tactagcgtg cctttgtaag 32640
cacaagctga tgagtacgaa cttatgtact cattcgtttt ggaagagaca ggtacgttaa 32700
tagttaatag cgtacttctt tttcttgctt tcgtggtatt cttgctagtt acactagcca 32760
tccttactgc gcttcgattg tgtgcgtact gctgcaatat tgttaacgtg agtcttgtaa 32820
aaccttcttt ttacgtttac tctcgtgtta aaaatctgaa ttcttctaga gttcctgatc 32880
ttctggtcta aacgaactaa atattatatt agtttttctg tttggaactt taattttagc 32940
catggcagat tccaacggta ctattaccgt tgaagagctt aaaaagctcc ttgaacaatg 33000
gaacctagta ataggtttcc tattccttac atggatttgt cttctacaat ttgcctatgc 33060
caacaggaat aggtttttgt atataattaa gttaattttc ctctggctgt tatggccagt 33120
aactttagct tgttttgtgc ttgctgctgt ttacagaata aattggatca ccggtggaat 33180
tgctatcgca atggcttgtc ttgtaggctt gatgtggctc agctacttca ttgcttcttt 33240
cagactgttt gcgcgtacgc gttccatgtg gtcattcaat ccagaaacta acattcttct 33300
caacgtgcca ctccatggca ctattctgac cagaccgctt ctagaaagtg aactcgtaat 33360
cggagctgtg atccttcgtg gacatcttcg tattgctgga caccatctag gacgctgtga 33420
catcaaggac ctgcctaaag aaatcactgt tgctacatca cgaacgcttt cttattacaa 33480
attgggagct tcgcagcgtg tagcaggtga ctcaggtttt gctgcataca gtcgctacag 33540
gattggcaac tataaattaa acacagacca ttccagtagc agtgacaata ttgctttgct 33600
tgtacagtaa gtgacaacag atgtttcatc tcgttgactt tcaggttact atagcagaga 33660
tattactaat tattatgagg acttttaaag tttccatttg gaatcttgat tacatcataa 33720
acctcataat taaaaattta tctaagtcac taactgagaa taaatattct caattagatg 33780
aagagcaacc aatggagatt gattaaacga acatgaaaat tattcttttc ttggcactga 33840
taacactcgc tacttgtgag ctttatcact accaagagtg tgttagaggt acaacagtac 33900
ttttaaaaga accttgctct tctggaacat acgagggcaa ttcaccattt catcctctag 33960
ctgataacaa atttgcactg acttgcttta gcactcaatt tgcttttgct tgtcctgacg 34020
gcgtaaaaca cgtctatcag ttacgtgcca gatcagtttc acctaaactg ttcatcagac 34080
aagaggaagt tcaagaactt tactctccaa tttttcttat tgttgcggca atagtgttta 34140
taacactttg cttcacactc aaaagaaaga cagaatgatt gaactttcat taattgactt 34200
ctatttgtgc tttttagcct ttctgctatt ccttgtttta attatgctta ttatcttttg 34260
gttctcactt gaactgcaag atcataatga aacttgtcac gcctaaacga acatgaaatt 34320
tcttgttttc ttaggaatca tcacaactgt agctgcattt caccaagaat gtagtttaca 34380
gtcatgtact caacatcaac catatgtagt tgatgacccg tgtcctattc acttctattc 34440
taaatggtat attagagtag gagctagaaa atcagcacct ttaattgaat tgtgcgtgga 34500
tgaggctggt tctaaatcac ccattcagta catcgatatc ggtaattata cagtttcctg 34560
tttacctttt acaattaatt gccaggaacc taaattgggt agtcttgtag tgcgttgttc 34620
gttctatgaa gactttttag agtatcatga cgttcgtgtt gttttagatt tcatctaaac 34680
gaacaaacta aaatgtctga taatggaccc caaaatcagc gaaatgcacc ccgcattacg 34740
tttggtggac cctcagattc aactggcagt aaccagaatg gagaacgcag tggggcgcga 34800
tcaaaacaac gtcggcccca aggtttaccc aataatactg cgtcttggtt caccgctctc 34860
actcaacatg gcaaggaaga ccttaaattc cctcgaggac aaggcgttcc aattaacacc 34920
aatagcagtc cagatgacca aattggctac taccgaagag ctaccagacg aattcgtggt 34980
ggtgacggta aaatgaaaga tctcagtcca agatggtatt tctactacct aggaactggg 35040
ccagaagctg gacttcccta tggtgctaac aaagacggca tcatatgggt tgcaactgag 35100
ggagccttga atacaccaaa agatcacatt ggcacccgca atcctgctaa caatgctgca 35160
atcgtgctac aacttcctca aggaacaaca ttgccaaaag gcttctacgc agaagggagc 35220
agaggcggca gtcaagcctc ttctcgttcc tcatcacgta gtcgcaacag ttcaagaaat 35280
tcaactccag gcagcagtag gggaacttct cctgctagaa tggctggcaa tggcggtgat 35340
gctgctcttg ctttgctgct gcttgacaga ttgaaccagc ttgagagcaa aatgtctggt 35400
aaaggccaac aacaacaagg ccaaactgtc actaagaaat ctgctgctga ggcttctaag 35460
aagcctcggc aaaaacgtac tgccactaaa gcatacaatg taacacaagc tttcggcaga 35520
cgtggtccag aacaaaccca aggaaatttt ggggaccagg aactaatcag acaaggaact 35580
gattacaaac attggccgca aattgcacaa tttgccccca gcgcttcagc gttcttcgga 35640
atgtcgcgca ttggcatgga agtcacacct tcgggaacgt ggttgaccta cacaggtgcc 35700
atcaaattgg atgacaaaga tccaaatttc aaagatcaag tcattttgct gaataagcat 35760
attgacgcat acaaaacatt cccaccaaca gagcctaaaa aggacaaaaa gaagaaggct 35820
gatgaaactc aagccttacc gcagagacag aagaaacagc aaactgtgac tcttcttcct 35880
gctgcagatt tggatgattt ctccaaacaa ttgcaacaat ccatgagcag tgctgactca 35940
actcaggcct aaactcatgc agaccacaca aggcagatgg gctatataaa cattttcgct 36000
tttccgttta cgatatatag tctactcttg tgcagaatga attctcgtaa ctacatagca 36060
caagtagatg tagttaactt taatctcaca tagcaatctt taatcagtgt gtaacattag 36120
ggaggacttg aaagagccac cacattttca ccgaggccac gcggagtacg atcgagtgta 36180
cagtgaacaa tgctagggag agctgcctat atggaagagc cctaatgtgt aaaattaatt 36240
ttagtagtgc tatccccatg tgattttaat agcttcttag gagaatgaca aaaaaaaaaa 36300
aaaaaaaaaa aaaaaaaaac tagcataacc ccttggggcc tctaaacggg tcttgagggg 36360
ttttttggcg gccgcttttt tcctt 36385
<210> 2
<211> 29674
<212> DNA
<213> cDNA(SARS-CoV-2)
<400> 2
attaaaggtt tataccttcc caggtaacaa accaaccaac tttcgatctc ttgtagatct 60
gttctctaaa cgaactttaa aatctgtgtg gctgtcactc ggctgcatgc ttagtgcact 120
cacgcagtat aattaataac taattactgt cgttgacagg acacgagtaa ctcgtctatc 180
ttctgcaggc tgcttacggt ttcgtccgtg ttgcagccga tcatcagcac atctaggttt 240
cgtccgggtg tgaccgaaag gtaagatgga gagccttgtc cctggtttca acgagaaaac 300
acacgtccaa ctcagtttgc ctgttttaca ggttcgcgac gtgctcgtac gtggctttgg 360
agactccgtg gaggaggtct tatcagaggc acgtcaacat cttaaagatg gcacttgtgg 420
cttagtagaa gttgaaaaag gcgttttgcc tcaacttgaa cagccctatg tgttcatcaa 480
acgttcggat gctcgaactg cacctcatgg tcatgttatg gttgagctgg tagcagaact 540
cgaaggcatt cagtacggtc gtagcggtga gacacttggt gtccttgtcc ctcatgtggg 600
cgaaatacca gtggcttacc gcaaggttct tcttcgtaag aacggtaata aaggagctgg 660
tggccatagt tacggcgccg atctaaagtc atttgactta ggcgacgagc ttggcactga 720
tccttatgaa gattttcaag aaaactggaa cactaaacat agcagtggtg ttacccgtga 780
actcatgcgt gagcttaacg gaggggcata cactcgctat gtcgataaca acttctgtgg 840
ccctgatggc taccctcttg agtgcattaa agaccttcta gcacgtgctg gtaaagcttc 900
atgcactttg tccgaacaac tggactttat tgacactaag aggggtgtat actgctgccg 960
tgaacatgag catgaaattg cttggtacac ggaacgttct gaaaagagct atgaattgca 1020
gacacctttt gaaattaaat tggcaaagaa atttgacacc ttcaatgggg aatgtccaaa 1080
ttttgtattt cccttaaatt ccataatcaa gactattcaa ccaagggttg aaaagaaaaa 1140
gcttgatggc tttatgggta gaattcgatc tgtctatcca gttgcgtcac caaatgaatg 1200
caaccaaatg tgcctttcaa ctctcatgaa gtgtgatcat tgtggtgaaa cttcatggca 1260
gacgggcgat tttgttaaag ccacttgcga attttgtggc actgagaatt tgactaaaga 1320
aggtgccact acttgtggtt acttacccca aaatgctgtt gttaaaattt attgtccagc 1380
atgtcacaat tcagaagtag gacctgagca tagtcttgcc gaataccata atgaatctgg 1440
cttgaaaacc attcttcgta agggtggtcg cactattgcc tttggaggct gtgtgttctc 1500
ttatgttggt tgccataaca agtgtgccta ttgggttcca cgtgctagcg ctaacatagg 1560
ttgtaaccat acaggtgttg ttggagaagg ttccgaaggt cttaatgaca accttcttga 1620
aatactccaa aaagagaaag tcaacatcaa tattgttggt gactttaaac ttaatgaaga 1680
gatcgccatt attttggcat ctttttctgc ttccacaagt gcttttgtgg aaactgtgaa 1740
aggtttggat tataaagcat tcaaacaaat tgttgaatcc tgtggtaatt ttaaagttac 1800
aaaaggaaaa gctaaaaaag gtgcctggaa tattggtgaa cagaaatcaa tactgagtcc 1860
tctttatgca tttgcatcag aggctgctcg tgttgtacga tcaattttct cccgcactct 1920
tgaaactgct caaaattctg tgcgtgtttt acagaaggcc gctataacaa tactagatgg 1980
aatttcacag tattcactga gactcattga tgctatgatg ttcacatctg atttggctac 2040
taacaatcta gttgtaatgg cctacattac aggtggtgtt gttcagttga cttcgcagtg 2100
gctaactaac atctttggca ctgtttatga aaaactcaaa cccgtccttg attggcttga 2160
agagaagttt aaggaaggtg tagagtttct tagagacggt tgggaaattg ttaaatttat 2220
ctcaacctgt gcttgtgaaa ttgtcggtgg acaaattgtc acctgtgcaa aggaaattaa 2280
ggagagtgtt cagacattct ttaagcttgt aaataaattt ttggctttgt gtgctgactc 2340
tatcattatt ggtggagcta aacttaaagc cttgaattta ggtgaaacat ttgtcacgca 2400
ctcaaaggga ttgtacagaa agtgtgttaa atccagagaa gaaactggcc tactcatgcc 2460
tctaaaagcc ccaaaagaaa ttatcttctt agagggagaa acacttccca cagaagtgtt 2520
aacagaggaa gttgtcttga aaactggtga tttacaacca ttagaacaac ctactagtga 2580
agctgttgaa gctccattgg ttggtacacc agtttgtatt aacgggctta tgttgctcga 2640
aatcaaagac acagaaaagt actgtgccct tgcacctaat atgatggtaa caaacaatac 2700
cttcacactc aaaggcggtg caccaacaaa ggttactttt ggtgatgaca ctgtgataga 2760
agtgcaaggt tacaagagtg tgaatatcac ttttgaactt gatgaaagga ttgataaagt 2820
acttaatgag aagtgctctg cctatacagt tgaactcggt acagaagtaa atgagttcgc 2880
ctgtgttgtg gcagatgctg tcataaaaac tttgcaacca gtatctgaat tacttacacc 2940
actgggcatt gatttagatg agtggagtat ggctacatac tacttatttg atgagtctgg 3000
tgagtttaaa ttggcttcac atatgtattg ttctttctac cctccagatg aggatgaaga 3060
agaaggtgat tgtgaagaag aagagtttga gccatcaact caatatgagt atggtactga 3120
agatgattac caaggtaaac ctttggaatt tggtgccact tctgctgctc ttcaacctga 3180
agaagagcaa gaagaagatt ggttagatga tgatagtcaa caaactgttg gtcaacaaga 3240
cggcagtgag gacaatcaga caactactat tcaaacaatt gttgaggttc aacctcaatt 3300
agagatggaa cttacaccag ttgttcagac tattgaagtg aatagtttta gtggttattt 3360
aaaacttact gacaatgtat acattaaaaa tgcagacatt gtggaagaag ctaaaaaggt 3420
aaaaccaaca gtggttgtta atgcagccaa tgtttacctt aaacatggag gaggtgttgc 3480
aggagcctta aataaggcta ctaacaatgc catgcaagtt gaatctgatg attacatagc 3540
tactaatgga ccacttaaag tgggtggtag ttgtgtttta agcggacaca atcttgctaa 3600
acactgtctt catgttgtcg gcctaaatgt taacaaaggt gaagacattc aacttcttaa 3660
gagtgcttat gaaaatttta atcagcacga agttctactt gcaccattat tatcagctgg 3720
tatttttggt gctgacccta tacattcttt aagagtttgt gtagatactg ttcgcacaaa 3780
tgtctactta gctgtctttg ataaaaatct ctatgacaaa cttgtttcaa gctttttgga 3840
aatgaagagt gaaaagcaag ttgaacaaaa gatcgctgag attcctaaag aggaagttaa 3900
gccatttata actgaaagta aaccttcagt tgaacagaga aaacaagatg ataagaaaat 3960
caaagcttgt gttgaagaag ttacaacaac tctggaagaa actaagttcc tcacagaaaa 4020
cttgttactt tatattgaca ttaatggcaa tcttcatcca gattctgcca ctcttgttag 4080
tgacattgac atcactttct taaagaaaga tgctccatat atagtgggtg atgttattca 4140
agagggtgtt ttaactgctg tggttatacc tactaaaaag gctggtggca ctactgaaat 4200
gctagcgaaa gctttgagaa aagtgccaac agacaattat ataaccactt acccgggtca 4260
gggtttaaat ggttacactg tagaggaggc aaagacagtg cttaaaaagt gtaaaagtgc 4320
cttttacatt ctaccatcta ttatctctaa tgagaagcaa gaaattcttg gaactgtttc 4380
ttggaatttg cgagaaatgc ttgcacatgc agaagaaaca cgcaaattaa tgcctgtctg 4440
tgtggaaact aaagccatag tttcaactat acagcgtaaa tataagggta ttaaaataca 4500
agagggtgtg gttgattatg gtgctagatt ttacttttac accagtaaaa caactgtagc 4560
gtcacttatc aacacactta acgatctaaa tgaaactctt gttacaatgc cacttggcta 4620
tgtaacacat ggcttaaatt tggaagaagc tgctcggtat atgagatctc tcaaagtgcc 4680
agctacagtt tctgtttctt cacctgatgc tgttacagcg tataatggtt atcttacttc 4740
ttcttctaaa acacctgaag aacattttat tgaaaccatc tcacttgctg gttcctataa 4800
agattggtcc tattctggac aatctacaca actaggtata gaatttctta agagaggtga 4860
taaaagtgta tattacacta gtaatcctac cacattccac ctagatggtg aagttatcac 4920
ctttgacaat cttaagacac ttctttcttt gagagaagtg aggactatta aggtgtttac 4980
aacagtagac aacattaacc tccacacgca agttgtggac atgtcaatga catatggaca 5040
acagtttggt ccaacttatt tggatggagc tgatgttact aaaataaaac ctcataattc 5100
acatgaaggt aaaacatttt atgttttacc taatgatgac actctacgtg ttgaggcttt 5160
tgagtactac cacacaactg atcctagttt tctgggtagg tacatgtcag cattaaatca 5220
cactaaaaag tggaaatacc cacaagttaa tggtttaact tctattaaat gggcagataa 5280
caactgttat cttgccactg cattgttaac actccaacaa atagagttga agtttaatcc 5340
acctgctcta caagatgctt attacagagc aagggctggt gaagctgcta acttttgtgc 5400
acttatctta gcctactgta ataagacagt aggtgagtta ggtgatgtta gagaaacaat 5460
gagttacttg tttcaacatg ccaatttaga ttcttgcaaa agagtcttga acgtggtgtg 5520
taaaacttgt ggacaacagc agacaaccct taagggtgta gaagctgtta tgtacatggg 5580
cacactttct tatgaacaat ttaagaaagg tgttcagata ccttgtacgt gtggtaaaca 5640
agctacaaaa tatctagtac aacaggagtc accttttgtt atgatgtcag caccacctgc 5700
tcagtatgaa cttaagcatg gtacatttac ttgtgctagt gagtacactg gtaattacca 5760
gtgtggtcac tataaacata taacttctaa agaaactttg tattgcatag acggtgcttt 5820
acttacaaag tcctcagaat acaaaggtcc tattacggat gttttctaca aagaaaacag 5880
ttacacaaca accataaaac cagttactta taaattggat ggtgttgttt gtacagaaat 5940
tgaccctaag ttggacaatt attataagaa agacaattct tatttcacag agcaaccaat 6000
tgatcttgta ccaaaccaac catatccaaa cgcaagcttc gataatttta agtttgtatg 6060
tgataatatc aaatttgctg atgatttaaa ccagttaact ggttataaga aacctgcttc 6120
aagagagctt aaagttacat ttttccctga cttaaatggt gatgtggtgg ctattgatta 6180
taaacactac acaccctctt ttaagaaagg agctaaattg ttacataaac ctattgtttg 6240
gcatgttaac aatgcaacta ataaagccac gtataaacca aatacctggt gtatacgttg 6300
tctttggagc acaaaaccag ttgaaacatc aaattcgttt gatgtactga agtcagagga 6360
cgcgcaggga atggataatc ttgcctgcga agagctaaaa ccagtctctg aagaagtagt 6420
ggaaaatcct accatacaga aagacgttct tgagtgtaat gtgaaaacta ccgaagttgt 6480
aggagacatt atacttaaac cagcaaataa tagtttaaaa attacagaag aggttggcca 6540
cacagatcta atggctgctt atgtagacaa ttctagtctt actattaaga aacctaatga 6600
attatctaga gtattaggtt tgaaaaccct tgctactcat ggtttagctg ctgttaatag 6660
tgtcccttgg gatactatag ctaattatgc taagcctttt cttaacaaag ttgttagtac 6720
aactactaac atagttacac ggtgtttaaa ccgtgtttgt actaattata tgccttattt 6780
ctttacttta ttgctacaat tgtgtacttt tactagaagt acaaattcta gaattaaagc 6840
atctatgccg actactatag caaagaatac tgttaagagt gtcggtaaat tttgtctaga 6900
ggcttcattt aattatttga agtcacctaa tttttctaaa ctgataaata ttataatttg 6960
gtttttacta ttaagtgttt gcctaggttc tttaatctac tcaaccgctg ctttaggtgt 7020
tttaatgtct aatttaggca tgccttctta ctgtactggt tacagagaag gctatttgaa 7080
ctctactaat gtcactattg caacctactg tactggttct ataccttgta gtgtttgtct 7140
tagtggttta gattctttag acacctatcc ttctttagaa actatacaaa ttaccatttc 7200
atcttttaaa tgggatttaa ctgcttttgg cttagttgca gagtggtttt tggcatatat 7260
tcttttcact aggtttttct atgtacttgg attggctgca atcatgcaat tgtttttcag 7320
ctattttgca gtacatttta ttagtaattc ttggcttatg tggttaataa ttaatcttgt 7380
acaaatggcc ccgatttcag ctatggttag aatgtacatc ttctttgcat cattttatta 7440
tgtatggaaa agttatgtgc atgttgtaga cggttgtaat tcatcaactt gtatgatgtg 7500
ttacaaacgt aatagagcaa caagagtcga atgtacaact attgttaatg gtgttagaag 7560
gtccttttat gtctatgcta atggaggtaa aggcttttgc aaactacaca attggaattg 7620
tgttaattgt gatacattct gtgctggtag tacatttatt agtgatgaag ttgcgagaga 7680
cttgtcacta cagtttaaaa gaccaataaa tcctactgac cagtcttctt acatcgttga 7740
tagtgttaca gtgaagaatg gttccatcca tctttacttt gataaagctg gtcaaaagac 7800
ttatgaaaga cattctctct ctcattttgt taacttagac aacctgagag ctaataacac 7860
taaaggttca ttgcctatta atgttatagt ttttgatggt aaatcaaaat gtgaagaatc 7920
atctgcaaaa tcagcgtctg tttactacag tcagcttatg tgtcaaccta tactgttact 7980
agatcaggca ttagtgtctg atgttggtga tagtgcggaa gttgcagtta aaatgtttga 8040
tgcttacgtt aatacgtttt catcaacttt taacgtacca atggaaaaac tcaaaacact 8100
agttgcaact gcagaagctg aacttgcaaa gaatgtgtcc ttagacaatg tcttatctac 8160
ttttatttca gcagctcggc aagggtttgt tgattcagat gtagaaacta aagatgttgt 8220
tgaatgtctt aaattgtcac atcaatctga catagaagtt actggcgata gttgtaataa 8280
ctatatgctc acctataaca aagttgaaaa catgacaccc cgtgaccttg gtgcttgtat 8340
tgactgtagt gcgcgtcata ttaatgcgca ggtagcaaaa agtcacaaca ttgctttgat 8400
atggaacgtt aaagatttca tgtcattgtc tgaacaacta cgaaaacaaa tacgtagtgc 8460
tgctaaaaag aataacttac cttttaagtt gacatgtgca actactagac aagttgttaa 8520
tgttgtaaca acaaagatag cacttaaggg tggtaaaatt gttaataatt ggttgaagca 8580
gttaattaaa gttacacttg tgttcctttt tgttgctgct attttctatt taataacacc 8640
tgttcatgtc atgtctaaac atactgactt ttcaagtgaa atcataggat acaaggctat 8700
tgatggtggt gtcactcgtg acatagcatc tacagatact tgttttgcta acaaacatgc 8760
tgattttgac acatggttta gccagcgtgg cggtagttat actaatgaca aagcttgccc 8820
attgattgct gcagtcataa caagagaagt gggttttgtc gtgcctggtt tgcctggcac 8880
gatattacgc acaactaatg gtgacttttt gcatttctta cctagagttt ttagtgcagt 8940
tggtaacatc tgttacacac catcaaaact tatagagtac actgactttg caacatcagc 9000
ttgtgttttg gctgctgaat gtacaatttt taaagatgct tctggtaagc cagtaccata 9060
ttgttatgat accaatgtac tagaaggttc tgttgcttat gaaagtttac gccctgacac 9120
acgttatgtg ctcatggatg gctctattat tcaatttcct aacacctacc ttgaaggttc 9180
tgttagagtg gtaacaactt ttgattctga gtactgtagg cacggcactt gtgaaagatc 9240
agaagctggt gtttgtgtat ctactagtgg tagatgggta cttaacaatg attattacag 9300
atctttacca ggagttttct gtggtgtaga tgctgtaaat ttacttacta atatgtttac 9360
accactaatt caacctattg gtgctttgga catatcagca tctatagtag ctggtggtat 9420
tgtagctatc gtagtaacat gccttgccta ctattttatg aggtttagaa gagcttttgg 9480
tgaatacagt catgtagttg cctttaatac tttactattc cttatgtcat tcactgtact 9540
ctgtttaaca ccagtttact cattcttacc tggtgtttat tctgttattt acttgtactt 9600
gacattttat cttactaatg atgtttcttt tttagcacat attcagtgga tggttatgtt 9660
cacaccttta gtacctttct ggataacaat tgcttatatc atttgtattt ccacaaagca 9720
tttctattgg ttctttagta attacctaaa gagacgtgta gtctttaatg gtgtttcctt 9780
tagtactttt gaagaagctg cgctgtgcac ctttttgtta aataaagaaa tgtatctaaa 9840
gttgcgtagt gatgtgctat tacctcttac gcaatataat agatacttag ctctttataa 9900
taagtacaag tattttagtg gagcaatgga tacaactagc tacagagaag ctgcttgttg 9960
tcatctcgca aaggctctca atgacttcag taactcaggt tctgatgttc tttaccaacc 10020
accacaaacc tctatcacct cagctgtttt gcagagtggt tttagaaaaa tggcattccc 10080
atctggtaaa gttgagggtt gtatggtaca agtaacttgt ggtacaacta cacttaacgg 10140
tctttggctt gatgacgtag tttactgtcc aagacatgtg atctgcacct ctgaagacat 10200
gcttaaccct aattatgaag atttactcat tcgtaagtct aatcataatt tcttggtaca 10260
ggctggtaat gttcaactca gggttattgg acattctatg caaaattgtg tacttaagct 10320
taaggttgat acagccaatc ctaagacacc taagtataag tttgttcgca ttcaaccagg 10380
acagactttt tcagtgttag cttgttacaa tggttcacca tctggtgttt accaatgtgc 10440
tatgaggccc aatttcacta ttaagggttc attccttaat ggttcatgtg gtagtgttgg 10500
ttttaacata gattatgact gtgtctcttt ttgttacatg caccatatgg aattaccaac 10560
tggagttcat gctggcacag acttagaagg taacttttat ggaccttttg ttgacaggca 10620
aacagcacaa gcagctggta cggacacaac tattacagtt aatgttttag cttggttgta 10680
cgctgctgtt ataaatggag acaggtggtt tctcaatcga tttaccacaa ctcttaatga 10740
ctttaacctt gtggctatga agtacaatta tgaacctcta acacaagacc atgttgacat 10800
actaggacct ctttctgctc aaactggaat tgccgtttta gatatgtgtg cttcattaaa 10860
agaattactg caaaatggta tgaatggacg taccatattg ggtagtgctt tattagaaga 10920
tgaatttaca ccttttgatg ttgttagaca atgctcaggt gttactttcc aaagtgcagt 10980
gaaaagaaca atcaagggta cacaccactg gttgttactc acaattttga cttcactttt 11040
agttttagtc cagagtactc aatggtcttt gttctttttt ttgtatgaaa atgccttttt 11100
accttttgct atgggtatta ttgctatgtc tgcttttgca atgatgtttg tcaaacataa 11160
gcatgcattt ctctgtttgt ttttgttacc ttctcttgcc actgtagctt attttaatat 11220
ggtctatatg cctgctagtt gggtgatgcg tattatgaca tggttggata tggttgatac 11280
tagtttgtct ggttttaagc taaaagactg tgttatgtat gcatcagctg tagtgttact 11340
aatccttatg acagcaagaa ctgtgtatga tgatggtgct aggagagtgt ggacacttat 11400
gaatgtcttg acactcgttt ataaagttta ttatggtaat gctttagatc aagccatttc 11460
catgtgggct cttataatct ctgttacttc taactactca ggtgtagtta caactgtcat 11520
gtttttggcc agaggtattg tttttatgtg tgttgagtat tgccctattt tcttcataac 11580
tggtaataca cttcagtgta taatgctagt ttattgtttc ttaggctatt tttgtacttg 11640
ttactttggc ctcttttgtt tactcaaccg ctactttaga ctgactcttg gtgtttatga 11700
ttacttagtt tctacacagg agtttagata tatgaattca cagggactac tcccacccaa 11760
gaatagcata gatgccttca aactcaacat taaattgttg ggtgttggtg gcaaaccttg 11820
tatcaaagta gccactgtac agtctaaaat gtcagatgta aagtgcacgt cagtagtctt 11880
actctcagtt ttgcaacaac tcagagtaga atcatcatct aaattgtggg ctcaatgtgt 11940
ccagttacac aatgacattc tcttagctaa agatactact gaagcctttg aaaaaatggt 12000
ttcactactt tctgttttgc tttccatgca gggtgctgta gacataaaca agctttgtga 12060
agaaatgctg gacaacaggg caaccttaca agctatagcc tcagagttta gttcccttcc 12120
atcatatgca gcttttgcta ctgctcaaga agcttatgag caggctgttg ctaatggtga 12180
ttctgaagtt gttcttaaaa agttgaagaa gtctttgaat gtggctaaat ctgaatttga 12240
ccgtgatgca gccatgcaac gtaagttgga aaagatggct gatcaagcta tgacccaaat 12300
gtataaacag gctagatctg aggacaagag ggcaaaagtt actagtgcta tgcagacaat 12360
gcttttcact atgcttagaa agttggataa tgatgcactc aacaacatta tcaacaatgc 12420
aagagatggt tgtgttccct tgaacataat acctcttaca acagcagcca aactaatggt 12480
tgtcatacca gactataaca catataaaaa tacgtgtgat ggtacaacat ttacttatgc 12540
atcagcattg tgggaaatcc aacaggttgt agatgcagat agtaaaattg ttcaacttag 12600
tgaaattagt atggacaatt cacctaattt agcatggcct cttattgtaa cagccttaag 12660
ggccaattct gctgtcaaat tacagaataa tgagcttagt cctgttgcac tacgacagat 12720
gtcttgtgct gccggtacta cacaaactgc ttgcactgat gacaatgcgt tagcttacta 12780
caacacaaca aagggaggta ggtttgtact tgcactgtta tccgatttac aggatttgaa 12840
atgggctaga ttccctaaga gtgatggaac tggtactatc tatacagaac tggaaccacc 12900
ttgtaggttt gttacagaca cacctaaagg tcctaaagtg aagtatttat actttattaa 12960
aggattaaac aacctaaata gaggtatggt acttggtagt ttagctgcca cagtacgtct 13020
acaagctggt aatgcaacag aagtgcctgc caattcaact gtattatctt tctgtgcttt 13080
tgctgtagat gctgctaaag cttacaaaga ttatctagct agtgggggac aaccaatcac 13140
taattgtgtt aagatgttgt gtacatacac tggtactggt caggcaataa cagttacacc 13200
ggaagccaat atggatcaag aatcctttgg tggtgcatcg tgttgtctgt actgccgttg 13260
ccacatagat catccaaatc ctaaaggatt ttgtgactta aaaggtaagt atgtacaaat 13320
acctacaact tgtgctaatg accctgtggg ttttacactt aaaaacacag tctgtaccgt 13380
ctgcggtatg tggaaaggtt atggctgtag ttgtgatcaa ctccgcgaac ccatgcttca 13440
gtcagctgat gcacaatcgt ttttaaacgg gtttgcggtg taagtgcagc ccgtcttaca 13500
ccgtgcggca caggcactag tactgatgtc gtatacaggg cttttgacat ctacaatgat 13560
aaagtagctg gttttgctaa attcctaaaa actaattgtt gtcgcttcca agaaaaggac 13620
gaagatgaca atttaattga ttcttacttt gtagttaaga gacacacttt ctctaactac 13680
caacatgaag aaacaattta taatttactt aaggattgtc cagctgttgc taaacatgac 13740
ttctttaagt ttagaataga cggtgacatg gtaccacata tatcacgtca acgtcttact 13800
aaatacacaa tggcagacct cgtctatgct ttaaggcatt ttgatgaagg taattgtgac 13860
acattaaaag aaatacttgt cacatacaat tgttgtgatg atgattattt caataaaaag 13920
gactggtatg attttgtaga aaacccagat atattacgcg tatacgccaa cttaggtgaa 13980
cgtgtacgcc aagctttgtt aaaaacagta caattctgtg atgccatgcg aaatgctggt 14040
attgttggtg tactgacatt agataatcaa gatctcaatg gtaactggta tgatttcggt 14100
gatttcatac aaaccacgcc aggtagtgga gttcctgttg tagattctta ttattcattg 14160
ttaatgccta tattaacctt gaccagggct ttaactgcag agtcacatgt tgacactgac 14220
ttaacaaagc cttacattaa gtgggatttg ttaaaatatg acttcacgga agagaggtta 14280
aaactctttg accgttattt taaatattgg gatcagacat accacccaaa ttgtgttaac 14340
tgtttggatg acagatgcat tctgcattgt gcaaacttta atgttttatt ctctacagtg 14400
ttcccaccta caagttttgg accactagtg agaaaaatat ttgttgatgg tgttccattt 14460
gtagtttcaa ctggatacca cttcagagag ctaggtgttg tacataatca ggatgtaaac 14520
ttacatagct ctagacttag ttttaaggaa ttacttgtgt atgctgctga ccctgctatg 14580
cacgctgctt ctggtaatct attactagat aaacgcacta cgtgcttttc agtagctgca 14640
cttactaaca atgttgcttt tcaaactgtc aaacccggta attttaacaa agacttctat 14700
gactttgctg tgtctaaggg tttctttaag gaaggaagtt ctgttgaatt aaaacacttc 14760
ttctttgctc aggatggtaa tgctgctatc agcgattatg actactatcg ttataatcta 14820
ccaacaatgt gtgatatcag acaactacta tttgtagttg aagttgttga taagtacttt 14880
gattgttacg atggtggctg tattaatgct aaccaagtca tcgtcaacaa cctagacaaa 14940
tcagctggtt ttccatctaa taaatggggt aaggctagac tttattatga ttcaatgagt 15000
tatgaggatc aagatgcact tttcgcatat acaaaacgta atgtcatccc tactataact 15060
caaatgaatc ttaagtatgc cattagtgca aagaatagag ctcgcaccgt agctggtgtc 15120
tctatctgta gtactatgac caatagacag tttcatcaaa aattattgaa atcaatagcc 15180
gccactagag gagctactgt agtaattgga acaagcaaat tctatggtgg ttggcacaac 15240
atgttaaaaa ctgtttatag tgatgtagaa aaccctcacc ttatgggttg ggattatcct 15300
aaatgtgata gagccatgcc taacatgctt agaattatgg cctcacttgt tcttgctcgc 15360
aaacatacaa cgtgttgtag cttgtcacac cgtttctata gattagctaa tgagtgtgct 15420
caagtattga gtgaaatggt catgtgtggc ggttcactat atgttaaacc aggtggaacc 15480
tcatcaggag atgccacaac tgcttatgct aatagtgttt ttaacatttg tcaagctgtc 15540
acggccaatg ttaatgcact tttatctact gatggtaaca aaattgccga taagtatgtc 15600
cgcaatttac aacacagact ttatgagtgt ctctatagaa atagagatgt tgacacagac 15660
tttgtgaatg agttttacgc atatttgcgt aaacatttct caatgatgat actctctgac 15720
gatgctgttg tgtgtttcaa tagcacttat gcatctcaag gtctagtggc tagcataaag 15780
aactttaagt cagttcttta ttatcaaaac aatgttttta tgtctgaagc aaaatgttgg 15840
actgagactg accttactaa aggacctcat gaattttgct ctcaacatac aatgctagtt 15900
aaacagggtg atgattatgt gtaccttcct tacccagatc catcaagaat cctaggggcc 15960
ggctgttttg tagatgatat cgtaaaaaca gatggtacac ttatgattga acggttcgtg 16020
tctttagcta tagatgctta cccacttact aaacatccta atcaggagta tgctgatgtc 16080
tttcatttgt acttacaata cataagaaag ctacatgatg agttaacagg acacatgtta 16140
gacatgtatt ctgttatgct tactaatgat aacacttcaa ggtattggga acctgagttt 16200
tatgaggcta tgtacacacc gcatacagtc ttacaggctg ttggggcttg tgttctttgc 16260
aattcacaga cttcattaag atgtggtgct tgcatacgta gaccattctt atgttgtaaa 16320
tgctgttacg accatgtcat atcaacatca cataaattag tcttgtctgt taatccgtat 16380
gtttgcaatg ctccaggttg tgatgtcaca gatgtgactc aactttactt aggaggtatg 16440
agctattatt gtaaatcaca taaaccaccc attagttttc cattgtgtgc taatggacaa 16500
gtttttggtt tatataaaaa tacatgtgtt ggtagcgata atgttactga ctttaatgca 16560
attgcaacat gtgactggac aaatgctggt gattacattt tagctaacac ctgtactgaa 16620
agactcaagc tttttgcagc agaaacgctc aaagctactg aggagacatt taaactgtct 16680
tatggtattg ctactgtacg tgaagtgctg tctgacagag aattacatct ttcatgggaa 16740
gttggtaaac ctagaccacc acttaaccga aattatgtct ttactggtta tcgtgtaact 16800
aaaaacagta aagtacaaat aggagagtac acctttgaaa aaggtgacta tggtgatgct 16860
gttgtttacc gaggtacaac aacttacaaa ttaaatgttg gtgattattt tgtgctgaca 16920
tcacatacag taatgccatt aagtgcacct acactagtgc cacaagagca ctatgttaga 16980
attactggct tatacccaac actcaatatc tcagatgagt tttctagcaa tgttgcaaat 17040
tatcaaaagg ttggtatgca aaagtattct acactccagg gaccacctgg tactggtaag 17100
agtcattttg ctattggcct agctctctac tacccttctg ctcgcatagt gtatacagct 17160
tgctctcatg ccgctgttga tgcactatgt gagaaggcat taaaatattt gcctatagat 17220
aaatgtagta gaattatacc tgcacgtgct cgtgtagagt gttttgataa attcaaagtg 17280
aattcaacat tagaacagta tgtcttttgt actgtaaatg tattgcctga gacgacagca 17340
gatatagttg tctttgatga aatttcaatg gccacaaatt atgatttgag tgttgtcaat 17400
gccagattac gtgctaagca ctatgtgtac attggcgacc ctgctcaatt acctgcacca 17460
cgcacattgc taactaaggg cacactagaa ccagaatatt tcaattcagt gtgtagactt 17520
atgaaaacta taggtccaga catgttcctc ggaacttgtc ggcgttgtcc tgctgaaatt 17580
gttgacactg tgagtgcttt ggtttatgat aataagctta aagcacataa agacaaatca 17640
gctcaatgct ttaaaatgtt ttataagggt gttatcacgc atgatgtttc atctgcaatt 17700
aacaggccac aaataggcgt ggtaagagaa ttccttacac gtaaccctgc ttggagaaaa 17760
gctgtcttta tttcacctta taattcacag aatgctgtag cctcaaagat tttgggacta 17820
ccaattcaaa ctgttgattc atcacagggc tcagaatatg actatgtcat attcactcaa 17880
accactgaaa cagctcactc ttgtaatgta aacagattta atgttgctat taccagagca 17940
aaagtaggca tactttgcat aatgtctgat agagaccttt atgacaagtt gcaatttaca 18000
agtcttgaaa ttccacgtag gaatgtggca actttacaag ctgaaaatgt aacaggactc 18060
tttaaagatt gtagtaaggt aatcactggg ttacatccta cacaggcacc tacacacctc 18120
agtgttgaca ctaaattcaa aactgaaggt ttatgtgttg acatacctgg catacctaag 18180
gacatgacct atagaagact catctctatg atgggtttta aaatgaatta tcaagttaat 18240
ggttacccta acatgtttat cacccgcgaa gaagctataa gacatgtacg tgcatggatt 18300
ggcttcgatg tcgaggggtg tcatgctact agagaagctg ttggtaccaa tttaccttta 18360
cagctaggtt tttctacagg tgttaaccta gttgctgtac ctacaggtta tgttgataca 18420
cctaataata cagatttctc cagagttagt gctaaaccac cgcctggaga tcaatttaaa 18480
cacctcatac cacttatgta caaaggactt ccttggaatg tagtgcgtat aaagattgta 18540
caaatgttaa gtgacacact taaaaatctc tctgacagag tcgtatttgt cttatgggca 18600
catggctttg agttgacatc tatgaagtat tttgtgaaaa taggacctga gcgcacctgt 18660
tgtctatgtg atagacgtgc cacatgcttt tccactgctt cagacactta tgcctgttgg 18720
catcattcta ttggatttga ttacgtctat aatccgttta tgattgatgt tcaacaatgg 18780
ggttttacag gtaacctaca aagcaaccat gatctgtatt gtcaagtcca tggtaatgca 18840
catatagcta gttgtgatgc aatcatgact aggtgtctag ctgtccacga gtgctttgtt 18900
aagcgtgttg actggactat tgaatatcct ataattggtg atgaactgaa gattaatgcg 18960
gcttgtagaa aggttcaaca catggttgtt aaagctgcat tattagcaga caaattccca 19020
gttcttcacg acattggtaa ccctaaagct attaagtgtg tacctcaagc tgatgtagaa 19080
tggaagttct atgatgcaca gccttgtagt gacaaagctt ataaaataga agaattattc 19140
tattcttatg ccacacattc tgacaaattc acagatggtg tatgcctatt ttggaattgc 19200
aatgtcgata gatatcctgc taattccatt gtttgtagat ttgacactag agtgctatct 19260
aaccttaact tgcctggttg tgatggtggc agtttgtatg taaataaaca tgcattccac 19320
acaccagctt ttgataaaag tgcttttgtt aatttaaaac aattaccatt tttctattac 19380
tctgacagtc catgtgagtc tcatggaaaa caagtagtgt cagatataga ttatgtacca 19440
ctaaagtctg ctacgtgtat aacacgttgc aatttaggtg gtgctgtctg tagacatcat 19500
gctaatgagt acagattgta tctcgatgct tataacatga tgatctcagc tggctttagc 19560
ttgtgggttt acaaacaatt tgatacttat aacctctgga acacttttac aagacttcag 19620
agtttagaaa atgtggcttt taatgttgta aataagggac actttgatgg acaacagggt 19680
gaagtaccag tttctatcat taataacact gtttacacaa aagttgatgg tgttgatgta 19740
gaattgtttg aaaataaaac aacattacct gttaatgtag catttgagct ttgggctaag 19800
cgcaacatta aaccagtacc agaggtgaaa atactcaata atttgggtgt ggacattgct 19860
gctaatactg tgatctggga ctacaaaaga gatgctccag cacatatatc tactattggt 19920
gtttgttcta tgactgacat agccaagaaa ccaactgaaa cgatttgtgc accactcact 19980
gtcttttttg atggtagagt tgatggtcaa gtagacttat ttagaaatgc ccgtaatggt 20040
gttcttatta cagaaggtag tgttaaaggt ttacaaccat ctgtaggtcc caaacaagct 20100
agtcttaatg gagtcacatt aattggagaa gccgtaaaaa cacagttcaa ttattataag 20160
aaagttgatg gtgttgtcca acaattacct gaaacttact ttactcagag tagaaattta 20220
caagaattta aacccaggag tcaaatggaa attgatttct tagaattagc tatggatgaa 20280
ttcattgaac ggtataaatt agaaggctat gccttcgaac atatcgttta tggagatttt 20340
agtcatagtc agttaggtgg tttacatcta ctgattggac tagctaaacg ttttaaggaa 20400
tcaccttttg aattagaaga ttttattcct atggacagta cagttaaaaa ctatttcata 20460
acagatgcgc aaacaggttc atctaagtgt gtgtgttctg ttattgattt attacttgat 20520
gattttgttg aaataataaa atcccaagat ttatctgtag tttctaaggt tgtcaaagtg 20580
actattgact atacagaaat ttcatttatg ctttggtgta aagatggcca tgtagaaaca 20640
ttttacccaa aattacaatc tagtcaagcg tggcaaccgg gtgttgctat gcctaatctt 20700
tacaaaatgc aaagaatgct attagaaaag tgtgaccttc aaaattatgg tgatagtgca 20760
acattaccta aaggcataat gatgaatgtc gcaaaatata ctcaactgtg tcaatattta 20820
aacacattaa cattagctgt accctataat atgagagtta tacattttgg tgctggttct 20880
gataaaggag ttgcaccagg tacagctgtt ttaagacagt ggttgcctac gggtacgctg 20940
cttgtcgatt cagatcttaa tgactttgtc tctgatgcag attcaacttt gattggtgat 21000
tgtgcaactg tacatacagc taataaatgg gatctcatta ttagtgatat gtacgaccct 21060
aagactaaaa atgttacaaa agaaaatgac tctaaagagg gttttttcac ttacatttgt 21120
gggtttatac aacaaaagct agctcttgga ggttccgtgg ctataaagat aacagaacat 21180
tcttggaatg ctgatcttta taagctcatg ggacacttcg catggtggac agcctttgtt 21240
actaatgtga atgcgtcatc atctgaagca tttttaattg gatgtaatta tcttggcaaa 21300
ccacgcgaac aaatagatgg ttatgtcatg catgcaaatt acatattttg gaggaataca 21360
aatccaattc agttgtcttc ctattcttta tttgacatga gtaaatttcc ccttaaatta 21420
aggggtactg ctgttatgtc tttaaaagaa ggtcaaatca atgatatgat tttatctctt 21480
cttagtaaag gtagacttat aattagagaa aacaacagag ttgttatttc tagtgatgtt 21540
cttgttaaca actaaacgaa caatgtttgt ttttcttgtt ttattgccac tagtctctag 21600
tcagtgtgtt aatcttacaa ccagaactca attaccccct gcatacacta attctttcac 21660
acgtggtgtt tattaccctg acaaagtttt cagatcctca gttttacatt caactcagga 21720
cttgttctta cctttctttt ccaatgttac ttggttccat gctatacatg tctctgggac 21780
caaaggtact aagaggtttg ataaccctgt cctaccattt aatgatggtg tttattttgc 21840
ttccactgag aagtctaaca taataagagg ctggattttt ggtactactt tagattcgaa 21900
gacccagtcc ctacttattg ttaataacgc tactaatgtt gttattaaag tctgtgaatt 21960
tcaattttgt aatgatccat ttttgggtgt ttattaccac aaaaacaaca aaagttggat 22020
ggaaagtgag ttcagagttt attctagtgc gaataattgc acttttgaat atgtctctca 22080
gccttttctt atggaccttg aaggaaaaca gggtaatttc aaaaatctta gggaatttgt 22140
gtttaagaat attgatggtt attttaaaat atattctaag cacacgccta ttaatttagt 22200
gcgtgatctc cctcagggtt tttcggcttt agaaccattg gtagatttgc caataggtat 22260
taacatcact aggtttcaaa ctttacttgc tttacataga agttatttga ctcctggtga 22320
ttcttcttca ggttggacag ctggtgctgc agcttattat gtgggttatc ttcaacctag 22380
gacttttcta ttaaaatata atgaaaatgg aaccattaca gatgctgtag actgtgcact 22440
tgaccctctc tcagaaacaa agtgtacgtt gaaatccttc actgtagaaa aaggaatcta 22500
tcaaacttct aactttagag tccaaccaac agaatctatt gttagatttc ctaatattac 22560
aaacttgtgc ccttttggtg aagtttttaa cgccaccaga tttgcatctg tttatgcttg 22620
gaacaggaag agaatcagca actgtgttgc tgattattct gtcctatata attccgcatc 22680
attttccact tttaagtgtt atggagtgtc tcctactaaa ttaaatgatc tctgctttac 22740
taatgtctat gcagattcat ttgtaattag aggtgatgaa gtcagacaaa tcgctccagg 22800
gcaaactgga aagattgctg attataatta taaattacca gatgatttta caggctgcgt 22860
tatagcttgg aattctaaca atcttgattc taaggttggt ggtaattata attacctgta 22920
tagattgttt aggaagtcta atctcaaacc ttttgagaga gatatttcaa ctgaaatcta 22980
tcaggccggt agcacacctt gtaatggtgt tgaaggtttt aattgttact ttcctttaca 23040
atcatatggt ttccaaccca ctaatggtgt tggttaccaa ccatacagag tagtagtact 23100
ttcttttgaa cttctacatg caccagcaac tgtttgtgga cctaaaaagt ctactaattt 23160
ggttaaaaac aaatgtgtca atttcaactt caatggttta acaggcacag gtgttcttac 23220
tgagtctaac aaaaagtttc tgcctttcca acaatttggc agagacattg ctgacactac 23280
tgatgctgtc cgtgatccac agacacttga gattcttgac attacaccat gttcttttgg 23340
tggtgtcagt gttataacac caggaacaaa tacttctaac caggttgctg ttctttatca 23400
ggatgttaac tgcacagaag tccctgttgc tattcatgca gatcaactta ctcctacttg 23460
gcgtgtttat tctacaggtt ctaatgtttt tcaaacacgt gcaggctgtt taataggggc 23520
tgaatatgtc aacaactcat atgagtgtga catacccatt ggtgcaggta tatgcgctag 23580
ttatcagact cagactaatt ctcctcggcg ggcacgtagt gtagctagtc aatccatcat 23640
tgcctacact atgtcacttg gtgcagaaaa ttcagttgct tactctaata actctattgc 23700
catacccaca aattttacta ttagtgttac cacagaaatt ctaccagtgt ctatgaccaa 23760
gacatcagta gattgtacaa tgtacatttg tggtgattca actgaatgca gcaatctttt 23820
gttgcaatat ggcagttttt gtacacaatt aaaccgtgct ttaactggaa tagctgttga 23880
acaagacaaa aacacccaag aagtttttgc acaagtcaaa caaatttaca aaacaccacc 23940
aattaaagat tttggtggtt ttaatttttc acaaatatta ccagatccat caaaaccaag 24000
caagaggtca tttattgaag atctactttt caacaaagtg acacttgcag atgctggctt 24060
catcaaacaa tatggtgatt gccttggtga tattgctgct agagacctca tttgtgcaca 24120
aaagtttaac ggccttactg ttttgccacc tttgctcaca gatgaaatga ttgctcaata 24180
cacttctgca ctgttagcgg gtacaatcac ttctggttgg acctttggtg caggtgctgc 24240
attacaaata ccatttgcta tgcaaatggc ttataggttt aatggtattg gagttacaca 24300
gaatgttctc tatgagaacc aaaaattgat tgccaaccaa tttaatagtg ctattggcaa 24360
aattcaagac tcactttctt ccacagcaag tgcacttgga aaacttcaag atgtggtcaa 24420
ccaaaatgca caagctttaa acacgcttgt taaacaactt agctccaatt ttggtgcaat 24480
ttcaagtgtt ttaaatgata tcctttcacg tcttgacaaa gttgaggctg aagtgcaaat 24540
tgataggttg atcacaggca gacttcaaag tttgcagaca tatgtgactc aacaattaat 24600
tagagctgca gaaatcagag cttctgctaa tcttgctgct actaaaatgt cagagtgtgt 24660
acttggacaa tcaaaaagag ttgatttttg tggaaagggc tatcatctta tgtccttccc 24720
tcagtcagca cctcatggtg tagtcttctt gcatgtgact tatgtccctg cacaagaaaa 24780
gaacttcaca actgctcctg ccatttgtca tgatggaaaa gcacactttc ctcgtgaagg 24840
tgtctttgtt tcaaatggca cacactggtt tgtaacacaa aggaattttt atgaaccaca 24900
aatcattact acagacaaca catttgtgtc tggtaactgt gatgttgtaa taggaattgt 24960
caacaacaca gtttatgatc ctttgcaacc tgaattagac tcattcaagg aggagttaga 25020
taaatatttt aagaatcata catcaccaga tgttgattta ggtgacatct ctggcattaa 25080
tgcttcagtt gtaaacattc aaaaagaaat tgaccgcctc aatgaggttg ccaagaattt 25140
aaatgaatct ctcatcgatc tccaagaact tggaaagtat gagcagtata taaaatggcc 25200
atggtacatt tggctaggtt ttatagctgg cttgattgcc atagtaatgg tgacaattat 25260
gctttgctgt atgaccagtt gctgtagttg tctcaagggc tgttgttctt gtggatcctg 25320
ctgcaaattt gatgaagacg actctgagcc agtgctcaaa ggagtcaaat tacattacac 25380
ataaacgaac ttatggattt gtttatgaga atcttcacaa ttggaactgt aactttgaag 25440
caaggtgaaa tcaaggatgc tactccttca gattttgttc gcgctactgc aacgataccg 25500
atacaagcct cactcccttt cggatggctt attgttggcg ttgcacttct tgctgttttt 25560
cagagcgctt ccaaaatcat aaccctcaaa aagagatggc aactagcact ctccaagggt 25620
gttcactttg tttgcaactt gctgttgttg tttgtaacag tttactcaca ccttttgctc 25680
gttgctgctg gccttgaagc cccttttctc tatctttatg ctttagtcta cttcttgcag 25740
agtataaact ttgtaagaat aataatgagg ctttggcttt gctggaaatg ccgttccaaa 25800
aacccattac tttatgatgc caactatttt ctttgctggc atactaattg ttacgactat 25860
tgtatacctt acaatagtgt aacttcttca attgtcatta cttcaggtga tggcacaaca 25920
agtcctattt ctgaacatga ctaccagatt ggtggttata ctgaaaaatg ggaatctgga 25980
gtaaaagact gtgttgtatt acacagttac ttcacttcag actattacca gctgtactca 26040
actcaattga gtacagacac tggtgttgaa catgttacct tcttcatcta caataaaatt 26100
gttgatgagc ctgaagaaca tgtccaaatt cacacaatcg acggttcatc cggagttgtt 26160
aatccagtaa tggaaccaat ttatgatgaa ccgacgacga ctactagcgt gcctttgtaa 26220
gcacaagctg atgagtacga acttatgtac tcattcgttt tggaagagac aggtacgtta 26280
atagttaata gcgtacttct ttttcttgct ttcgtggtat tcttgctagt tacactagcc 26340
atccttactg cgcttcgatt gtgtgcgtac tgctgcaata ttgttaacgt gagtcttgta 26400
aaaccttctt tttacgttta ctctcgtgtt aaaaatctga attcttctag agttcctgat 26460
cttctggtct aaacgaacta aatattatat tagtttttct gtttggaact ttaattttag 26520
ccatggcaga ttccaacggt actattaccg ttgaagagct taaaaagctc cttgaacaat 26580
ggaacctagt aataggtttc ctattcctta catggatttg tcttctacaa tttgcctatg 26640
ccaacaggaa taggtttttg tatataatta agttaatttt cctctggctg ttatggccag 26700
taactttagc ttgttttgtg cttgctgctg tttacagaat aaattggatc accggtggaa 26760
ttgctatcgc aatggcttgt cttgtaggct tgatgtggct cagctacttc attgcttctt 26820
tcagactgtt tgcgcgtacg cgttccatgt ggtcattcaa tccagaaact aacattcttc 26880
tcaacgtgcc actccatggc actattctga ccagaccgct tctagaaagt gaactcgtaa 26940
tcggagctgt gatccttcgt ggacatcttc gtattgctgg acaccatcta ggacgctgtg 27000
acatcaagga cctgcctaaa gaaatcactg ttgctacatc acgaacgctt tcttattaca 27060
aattgggagc ttcgcagcgt gtagcaggtg actcaggttt tgctgcatac agtcgctaca 27120
ggattggcaa ctataaatta aacacagacc attccagtag cagtgacaat attgctttgc 27180
ttgtacagta agtgacaaca gatgtttcat ctcgttgact ttcaggttac tatagcagag 27240
atattactaa ttattatgag gacttttaaa gtttccattt ggaatcttga ttacatcata 27300
aacctcataa ttaaaaattt atctaagtca ctaactgaga ataaatattc tcaattagat 27360
gaagagcaac caatggagat tgattaaacg aacatgaaaa ttattctttt cttggcactg 27420
ataacactcg ctacttgtga gctttatcac taccaagagt gtgttagagg tacaacagta 27480
cttttaaaag aaccttgctc ttctggaaca tacgagggca attcaccatt tcatcctcta 27540
gctgataaca aatttgcact gacttgcttt agcactcaat ttgcttttgc ttgtcctgac 27600
ggcgtaaaac acgtctatca gttacgtgcc agatcagttt cacctaaact gttcatcaga 27660
caagaggaag ttcaagaact ttactctcca atttttctta ttgttgcggc aatagtgttt 27720
ataacacttt gcttcacact caaaagaaag acagaatgat tgaactttca ttaattgact 27780
tctatttgtg ctttttagcc tttctgctat tccttgtttt aattatgctt attatctttt 27840
ggttctcact tgaactgcaa gatcataatg aaacttgtca cgcctaaacg aacatgaaat 27900
ttcttgtttt cttaggaatc atcacaactg tagctgcatt tcaccaagaa tgtagtttac 27960
agtcatgtac tcaacatcaa ccatatgtag ttgatgaccc gtgtcctatt cacttctatt 28020
ctaaatggta tattagagta ggagctagaa aatcagcacc tttaattgaa ttgtgcgtgg 28080
atgaggctgg ttctaaatca cccattcagt acatcgatat cggtaattat acagtttcct 28140
gtttaccttt tacaattaat tgccaggaac ctaaattggg tagtcttgta gtgcgttgtt 28200
cgttctatga agacttttta gagtatcatg acgttcgtgt tgttttagat ttcatctaaa 28260
cgaacaaact aaaatgtctg ataatggacc ccaaaatcag cgaaatgcac cccgcattac 28320
gtttggtgga ccctcagatt caactggcag taaccagaat ggagaacgca gtggggcgcg 28380
atcaaaacaa cgtcggcccc aaggtttacc caataatact gcgtcttggt tcaccgctct 28440
cactcaacat ggcaaggaag accttaaatt ccctcgagga caaggcgttc caattaacac 28500
caatagcagt ccagatgacc aaattggcta ctaccgaaga gctaccagac gaattcgtgg 28560
tggtgacggt aaaatgaaag atctcagtcc aagatggtat ttctactacc taggaactgg 28620
gccagaagct ggacttccct atggtgctaa caaagacggc atcatatggg ttgcaactga 28680
gggagccttg aatacaccaa aagatcacat tggcacccgc aatcctgcta acaatgctgc 28740
aatcgtgcta caacttcctc aaggaacaac attgccaaaa ggcttctacg cagaagggag 28800
cagaggcggc agtcaagcct cttctcgttc ctcatcacgt agtcgcaaca gttcaagaaa 28860
ttcaactcca ggcagcagta ggggaacttc tcctgctaga atggctggca atggcggtga 28920
tgctgctctt gctttgctgc tgcttgacag attgaaccag cttgagagca aaatgtctgg 28980
taaaggccaa caacaacaag gccaaactgt cactaagaaa tctgctgctg aggcttctaa 29040
gaagcctcgg caaaaacgta ctgccactaa agcatacaat gtaacacaag ctttcggcag 29100
acgtggtcca gaacaaaccc aaggaaattt tggggaccag gaactaatca gacaaggaac 29160
tgattacaaa cattggccgc aaattgcaca atttgccccc agcgcttcag cgttcttcgg 29220
aatgtcgcgc attggcatgg aagtcacacc ttcgggaacg tggttgacct acacaggtgc 29280
catcaaattg gatgacaaag atccaaattt caaagatcaa gtcattttgc tgaataagca 29340
tattgacgca tacaaaacat tcccaccaac agagcctaaa aaggacaaaa agaagaaggc 29400
tgatgaaact caagccttac cgcagagaca gaagaaacag caaactgtga ctcttcttcc 29460
tgctgcagat ttggatgatt tctccaaaca attgcaacaa tccatgagca gtgctgactc 29520
aactcaggcc taaactcatg cagaccacac aaggcagatg ggctatataa acattttcgc 29580
ttttccgttt acgatatata gtctactctt gtgcagaatg aattctcgta actacatagc 29640
acaagtagat gtagttaact ttaatctcac atag 29674
<210> 3
<211> 6410
<212> DNA
<213> Artificial sequence ()
<400> 3
ccttaattaa gtcgacagcg acacacttgc atcggatgca gcccggttaa cgtgccggca 60
cggcctgggt aaccaggtat tttgtccaca taaccgtgcg caaaatgttg tggataagca 120
ggacacagca gcaatccaca gcaggcatac aaccgcacac cgaggttact ccgttctaca 180
ggttacgacg acatgtcaat acttgccctt gacaggcatt gatggaatcg tagtctcacg 240
ctgatagtct gatcgacaat acaagtggga ccgtggtccc agaccgataa tcagaccgac 300
aacacgagtg ggatcgtggt cccagactaa taatcagacc gacgatacga gtgggaccgt 360
ggtcccagac taataatcag accgacgata cgagtgggac cgtggttcca gactaataat 420
cagaccgacg atacgagtgg gaccgtggtc ccagactaat aatcagaccg acgatacgag 480
tgggaccatg gtcccagact aataatcaga ccgacgatac gagtgggacc gtggtcccag 540
tctgattatc agaccgacga tacgagtggg accgtggtcc cagactaata atcagaccga 600
cgatacgagt gggaccgtgg tcccagacta ataatcagac cgacgatacg agtgggaccg 660
tggtcccagt ctgattatca gaccgacgat acaagtggaa cagtgggccc agagagaata 720
ttcaggccag ttatgctttc tggcctgtaa caaaggacat taagtaaaga cagataaacg 780
tagactaaaa cgtggtcgca tcagggtgct ggcttttcaa gttccttaag aatggcctca 840
attttctcta tacactcagt tggaacacga gacctgtcca ggttaagcac cattttatcg 900
cccttataca atactgtcgc tccaggagca aactgatgtc gtgagcttaa actagttctt 960
gatgcagatg acgttttaag cacagaagtt aaaagagtga taacttcttc agcttcaaat 1020
atcaccccag cttttttctg ctcatgaagg ttagatgcct gctgcttaag taattcctct 1080
ttatctgtaa aggctttttg aagtgcatca cctgaccggg cagatagttc accggggtga 1140
gaaaaaagag caacaactga tttaggcaat ttggcggtgt tgatacagcg ggtaataatc 1200
ttacgtgaaa tattttccgc atcagccagc gcagaaatat ttccagcaaa ttcattctgc 1260
aatcggcttg cataacgctg accacgttca taagcacttg ttgggcgata atcgttaccc 1320
aatctggata atgcagccat ctgctcatca tccagctcgc caaccagaac acgataatca 1380
ctttcggtaa gtgcagcagc tttacgacgg cgactcccat cggcaatttc tatgacacca 1440
gatactcttc gaccgaacgc cggtgtctgt tgaccagtca gtagaaaaga agggatgaga 1500
tcatccagtg cgtcctcagt aagcagctcc tggtcacgtt cattacctga ccatacccga 1560
gaggtcttct caacactatc accccggagc acttcaagag taaacttcac atcccgacca 1620
catacaggca aagtaatggc attaccgcga gccattactc ctacgcgcgc aattaacgaa 1680
tccaccatcg gggcagctgg tgtcgataac gaagtatctt caaccggttg agtattgagc 1740
gtatgttttg gaataacagg cgcacgcttc attatctaat ctcccagcgt ggtttaatca 1800
gacgatcgaa aatttcattg cagacaggtt cccaaataga aagagcattt ctccaggcac 1860
cagttgaaga gcgttgatca atggcctgtt caaaaacagt tctcatccgg atctgacctt 1920
taccaacttc atccgtttca cgtacaacat tttttagaac catgcttccc caggcatccc 1980
gaatttgctc ctccatccac ggggactgag agccattact attgctgtat ttggtaagca 2040
aaatacgtac atcaggctcg aaccctttaa gatcaacgtt cttgagcaga tcacgaagca 2100
tatcgaaaaa ctgcagtgcg gaggtgtagt caaacaactc agcaggcgtg ggaacaatca 2160
gcacatcagc agcacatacg acattaatcg tgccgatacc caggttaggc gcgctgtcaa 2220
taactatgac atcatagtca tgagcaacag tttcaatggc cagtcggagc atcaggtgtg 2280
gatcggtggg cagtttacct tcatcaaatt tgcccattaa ctcagtttca atacggtgca 2340
gagccagaca ggaaggaata atgtcaagcc ccggccagca agtgggcttt attgcataag 2400
tgacatcgtc cttttcccca agatagaaag gcaggagagt gtcttctgca tgaatatgaa 2460
gatctggtac ccatccgtga tacattgagg ctgttccctg ggggtcgtta ccttccacga 2520
gcaaaacacg tagccccttc agagccagat cctgagcaag atgaacagaa actgaggttt 2580
tgtaaacgcc acctttatgg gcagcaaccc cgatcaccgg tggaaatacg tcttcagcac 2640
gtcgcaatcg cgtaccaaac acatcacgca tatgattaat ttgttcaatt gtataaccaa 2700
cacgttgctc aacccgtcct cgaatttcca tatccgggtg cggtagtcgc cctgctttct 2760
cggcatctct gatagcctga gaagaaaccc caactaaatc cgctgcttca cctattctcc 2820
agcgccgggt tattttcctc gcttccgggc tgtcatcatt aaactgtgca atggcgatag 2880
ccttcgtcat ttcatgacca gcgtttatgc actggttaag tgtttccatg agtttcattc 2940
tgaacatcct ttaatcattg ctttgcgttt ttttattaaa tcttgcaatt tactgcaaag 3000
caacaacaaa atcgcaaagt catcaaaaaa ccgcaaagtt gtttaaaata agagcaacac 3060
tacaaaagga gataagaaga gcacatacct cagtcactta ttatcactag cgctcgccgc 3120
agccgtgtaa ccgagcatag cgagcgaact ggcgaggaag caaagaagaa ctgttctgtc 3180
agatagctct tacgctcagc gcaagaagaa atatccaccg tgggaaaaac tccaggtaga 3240
ggtacacacg cggatagcca attcagagta ataaactgtg ataatcaacc ctcatcaatg 3300
atgacgaact aacccccgat atcaggtcac atgacgaagg gaaagagaag gaaatcaact 3360
gtgacaaact gccctcaaat ttggcttcct taaaaattac agttcaaaaa gtatgagaaa 3420
atccatgcag gctgaaggaa acagcaaaac tgtgacaaat taccctcagt aggtcagaac 3480
aaatgtgacg aaccaccctc aaatctgtga cagataaccc tcagactatc ctgtcgtcat 3540
ggaagtgata tcgcggaagg aaaatacgat atgagtcgtc tggcggcctt tctttttctc 3600
aatgtatgag aggcgcattg gagttctgct gttgatctca ttaacacaga cctgcaggaa 3660
gcggcggcgg aagtcaggca tacgctggta actttgaggc agctggtaac gctctatgat 3720
ccagtcgatt ttcagagaga cgatgcctga gccatccggc ttacgatact gacacaggga 3780
ttcgtataaa cgcatggcat acggattggt gatttctttt gtttcactaa gccgaaactg 3840
cgtaaaccgg ttctgtaacc cgataaagaa gggaatgaga tatgggttga tatgtacact 3900
gtaaagccct ctggatggac tgtgcgcacg tttgataaac caaggaaaag attcatagcc 3960
tttttcatcg ccggcatcct cttcagggcg ataaaaaacc acttccttcc ccgcgaaact 4020
cttcaatgcc tgccgtatat ccttactggc ttccgcagag gtcaatccga atatttcagc 4080
atatttagca acatggatct cgcagatacc gtcatgttcc tgtagggtgc catcagattt 4140
tctgatctgg tcaacgaaca gatacagcat acgtttttga tcccgggaga gactatatgc 4200
cgcctcagtg aggtcgtttg actggacgat tcgcgggcta tttttacgtt tcttgtgatt 4260
gataaccgct gtttccgcca tgacagatcc atgtgaagtg tgacaagttt ttagattgtc 4320
acactaaata aaaaagagtc aataagcagg gataactttg tgaaaaaaca gcttcttctg 4380
agggcaattt gtcacagggt taagggcaat ttgtcacaga caggactgtc atttgagggt 4440
gatttgtcac actgaaaggg caatttgtca caacaccttc tctagaacca gcatggataa 4500
aggcctacaa ggcgctctaa aaaagaagat ctaaaaacta taaaaaaaat aattataaaa 4560
atatccccgt ggataagtgg ataaccccaa gggaagtttt ttcaggcatc gtgtgtaagc 4620
agaatatata agtgctgttc cctggtgctt cctcgctcac tcgagggctt cgccctgtcg 4680
ctcgactgcg gcgagcacta ctggctgtaa aaggacagac cacatcatgg ttctgtgttc 4740
attaggttgt tctgtccatt gctgacataa tccgctccac ttcaacgtaa caccgcacga 4800
agatttctat tgttcctgaa ggcatattca aatcgttttc gttaccgctt gcaggcatca 4860
tgacagaaca ctacttccta taaacgctac acaggctcct gagattaata atgcggatct 4920
ctacgataat gggagatttt cccgactgtt tcgttcgctt ctcagtggat aacagccagc 4980
ttctctgttt aacagacaaa aacagcatat ccactcagtt ccacatttcc atataaaggc 5040
caaggcattt attctcagga taattgtttc agcatcgcaa ccgcatcaga ctccggcatc 5100
gcaaactgca cccggtgccg ggcagccaca tccagcgcaa aaaccttcgt gtagacttcc 5160
gttgaactga tggacttatg tcccatcagg ctttgcagaa ctttcagcgg tataccggca 5220
tacagcatgt gcatcgcata ggaatggcgg aacgtatgtg gtgtgaccgg aacagagaac 5280
gtcacaccgt cagcagcagc ggcggcaacc gcctccccaa tccaggtcct gaccgttctg 5340
tccgtcactt cccagatccg cgctttctct gtccttcctg tgcgacggtt acgccgctcc 5400
atgagcttat cgcgaataaa tacctgtgac ggaagatcac ttcgcagaat aaataaatcc 5460
tggtgtccct gttgataccg ggaagccctg ggccaacttt tggcgaaaat gagacgttga 5520
tcggcacgta agaggttcca actttcacca taatgaaata agatcactac cgggcgtatt 5580
ttttgagtta tcgagatttt caggagctaa ggaagctaaa atggagaaaa aaatcactgg 5640
atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc 5700
agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac 5760
cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat 5820
gaatgctcat ccggaattcc gtatggcaat gaaagacggt gagctggtga tatgggatag 5880
tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat cgctctggag 5940
tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg tggcgtgtta 6000
cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt tcgtctcagc 6060
caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg acaacttctt 6120
cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct 6180
ggcgattcag gttcatcatg ccgtttgtga tggcttccat gtcggcagaa tgcttaatga 6240
attacaacag tactgcgatg agtggcaggg cggggcgtaa tttttttaag gcagttattg 6300
gtgcccttaa acgcctggtt gctacgcctg aataagtgat aataagcgga tgaatggcag 6360
aaattcgatg ataagctgtc aaacatgaga attggtcgac ttaattaagg 6410
<210> 4
<211> 33
<212> DNA
<213> Artificial sequence ()
<400> 4
ccttaattaa gtcgaccaat tctcatgttt gac 33
<210> 5
<211> 32
<212> DNA
<213> Artificial sequence ()
<400> 5
ccttaattaa gtcgacagcg acacacttgc at 32
<210> 6
<211> 6418
<212> DNA
<213> Artificial sequence ()
<400> 6
aaggaaaaaa gcggccgcgt cgacagcgac acacttgcat cggatgcagc ccggttaacg 60
tgccggcacg gcctgggtaa ccaggtattt tgtccacata accgtgcgca aaatgttgtg 120
gataagcagg acacagcagc aatccacagc aggcatacaa ccgcacaccg aggttactcc 180
gttctacagg ttacgacgac atgtcaatac ttgcccttga caggcattga tggaatcgta 240
gtctcacgct gatagtctga tcgacaatac aagtgggacc gtggtcccag accgataatc 300
agaccgacaa cacgagtggg atcgtggtcc cagactaata atcagaccga cgatacgagt 360
gggaccgtgg tcccagacta ataatcagac cgacgatacg agtgggaccg tggttccaga 420
ctaataatca gaccgacgat acgagtggga ccgtggtccc agactaataa tcagaccgac 480
gatacgagtg ggaccatggt cccagactaa taatcagacc gacgatacga gtgggaccgt 540
ggtcccagtc tgattatcag accgacgata cgagtgggac cgtggtccca gactaataat 600
cagaccgacg atacgagtgg gaccgtggtc ccagactaat aatcagaccg acgatacgag 660
tgggaccgtg gtcccagtct gattatcaga ccgacgatac aagtggaaca gtgggcccag 720
agagaatatt caggccagtt atgctttctg gcctgtaaca aaggacatta agtaaagaca 780
gataaacgta gactaaaacg tggtcgcatc agggtgctgg cttttcaagt tccttaagaa 840
tggcctcaat tttctctata cactcagttg gaacacgaga cctgtccagg ttaagcacca 900
ttttatcgcc cttatacaat actgtcgctc caggagcaaa ctgatgtcgt gagcttaaac 960
tagttcttga tgcagatgac gttttaagca cagaagttaa aagagtgata acttcttcag 1020
cttcaaatat caccccagct tttttctgct catgaaggtt agatgcctgc tgcttaagta 1080
attcctcttt atctgtaaag gctttttgaa gtgcatcacc tgaccgggca gatagttcac 1140
cggggtgaga aaaaagagca acaactgatt taggcaattt ggcggtgttg atacagcggg 1200
taataatctt acgtgaaata ttttccgcat cagccagcgc agaaatattt ccagcaaatt 1260
cattctgcaa tcggcttgca taacgctgac cacgttcata agcacttgtt gggcgataat 1320
cgttacccaa tctggataat gcagccatct gctcatcatc cagctcgcca accagaacac 1380
gataatcact ttcggtaagt gcagcagctt tacgacggcg actcccatcg gcaatttcta 1440
tgacaccaga tactcttcga ccgaacgccg gtgtctgttg accagtcagt agaaaagaag 1500
ggatgagatc atccagtgcg tcctcagtaa gcagctcctg gtcacgttca ttacctgacc 1560
atacccgaga ggtcttctca acactatcac cccggagcac ttcaagagta aacttcacat 1620
cccgaccaca tacaggcaaa gtaatggcat taccgcgagc cattactcct acgcgcgcaa 1680
ttaacgaatc caccatcggg gcagctggtg tcgataacga agtatcttca accggttgag 1740
tattgagcgt atgttttgga ataacaggcg cacgcttcat tatctaatct cccagcgtgg 1800
tttaatcaga cgatcgaaaa tttcattgca gacaggttcc caaatagaaa gagcatttct 1860
ccaggcacca gttgaagagc gttgatcaat ggcctgttca aaaacagttc tcatccggat 1920
ctgaccttta ccaacttcat ccgtttcacg tacaacattt tttagaacca tgcttcccca 1980
ggcatcccga atttgctcct ccatccacgg ggactgagag ccattactat tgctgtattt 2040
ggtaagcaaa atacgtacat caggctcgaa ccctttaaga tcaacgttct tgagcagatc 2100
acgaagcata tcgaaaaact gcagtgcgga ggtgtagtca aacaactcag caggcgtggg 2160
aacaatcagc acatcagcag cacatacgac attaatcgtg ccgataccca ggttaggcgc 2220
gctgtcaata actatgacat catagtcatg agcaacagtt tcaatggcca gtcggagcat 2280
caggtgtgga tcggtgggca gtttaccttc atcaaatttg cccattaact cagtttcaat 2340
acggtgcaga gccagacagg aaggaataat gtcaagcccc ggccagcaag tgggctttat 2400
tgcataagtg acatcgtcct tttccccaag atagaaaggc aggagagtgt cttctgcatg 2460
aatatgaaga tctggtaccc atccgtgata cattgaggct gttccctggg ggtcgttacc 2520
ttccacgagc aaaacacgta gccccttcag agccagatcc tgagcaagat gaacagaaac 2580
tgaggttttg taaacgccac ctttatgggc agcaaccccg atcaccggtg gaaatacgtc 2640
ttcagcacgt cgcaatcgcg taccaaacac atcacgcata tgattaattt gttcaattgt 2700
ataaccaaca cgttgctcaa cccgtcctcg aatttccata tccgggtgcg gtagtcgccc 2760
tgctttctcg gcatctctga tagcctgaga agaaacccca actaaatccg ctgcttcacc 2820
tattctccag cgccgggtta ttttcctcgc ttccgggctg tcatcattaa actgtgcaat 2880
ggcgatagcc ttcgtcattt catgaccagc gtttatgcac tggttaagtg tttccatgag 2940
tttcattctg aacatccttt aatcattgct ttgcgttttt ttattaaatc ttgcaattta 3000
ctgcaaagca acaacaaaat cgcaaagtca tcaaaaaacc gcaaagttgt ttaaaataag 3060
agcaacacta caaaaggaga taagaagagc acatacctca gtcacttatt atcactagcg 3120
ctcgccgcag ccgtgtaacc gagcatagcg agcgaactgg cgaggaagca aagaagaact 3180
gttctgtcag atagctctta cgctcagcgc aagaagaaat atccaccgtg ggaaaaactc 3240
caggtagagg tacacacgcg gatagccaat tcagagtaat aaactgtgat aatcaaccct 3300
catcaatgat gacgaactaa cccccgatat caggtcacat gacgaaggga aagagaagga 3360
aatcaactgt gacaaactgc cctcaaattt ggcttcctta aaaattacag ttcaaaaagt 3420
atgagaaaat ccatgcaggc tgaaggaaac agcaaaactg tgacaaatta ccctcagtag 3480
gtcagaacaa atgtgacgaa ccaccctcaa atctgtgaca gataaccctc agactatcct 3540
gtcgtcatgg aagtgatatc gcggaaggaa aatacgatat gagtcgtctg gcggcctttc 3600
tttttctcaa tgtatgagag gcgcattgga gttctgctgt tgatctcatt aacacagacc 3660
tgcaggaagc ggcggcggaa gtcaggcata cgctggtaac tttgaggcag ctggtaacgc 3720
tctatgatcc agtcgatttt cagagagacg atgcctgagc catccggctt acgatactga 3780
cacagggatt cgtataaacg catggcatac ggattggtga tttcttttgt ttcactaagc 3840
cgaaactgcg taaaccggtt ctgtaacccg ataaagaagg gaatgagata tgggttgata 3900
tgtacactgt aaagccctct ggatggactg tgcgcacgtt tgataaacca aggaaaagat 3960
tcatagcctt tttcatcgcc ggcatcctct tcagggcgat aaaaaaccac ttccttcccc 4020
gcgaaactct tcaatgcctg ccgtatatcc ttactggctt ccgcagaggt caatccgaat 4080
atttcagcat atttagcaac atggatctcg cagataccgt catgttcctg tagggtgcca 4140
tcagattttc tgatctggtc aacgaacaga tacagcatac gtttttgatc ccgggagaga 4200
ctatatgccg cctcagtgag gtcgtttgac tggacgattc gcgggctatt tttacgtttc 4260
ttgtgattga taaccgctgt ttccgccatg acagatccat gtgaagtgtg acaagttttt 4320
agattgtcac actaaataaa aaagagtcaa taagcaggga taactttgtg aaaaaacagc 4380
ttcttctgag ggcaatttgt cacagggtta agggcaattt gtcacagaca ggactgtcat 4440
ttgagggtga tttgtcacac tgaaagggca atttgtcaca acaccttctc tagaaccagc 4500
atggataaag gcctacaagg cgctctaaaa aagaagatct aaaaactata aaaaaaataa 4560
ttataaaaat atccccgtgg ataagtggat aaccccaagg gaagtttttt caggcatcgt 4620
gtgtaagcag aatatataag tgctgttccc tggtgcttcc tcgctcactc gagggcttcg 4680
ccctgtcgct cgactgcggc gagcactact ggctgtaaaa ggacagacca catcatggtt 4740
ctgtgttcat taggttgttc tgtccattgc tgacataatc cgctccactt caacgtaaca 4800
ccgcacgaag atttctattg ttcctgaagg catattcaaa tcgttttcgt taccgcttgc 4860
aggcatcatg acagaacact acttcctata aacgctacac aggctcctga gattaataat 4920
gcggatctct acgataatgg gagattttcc cgactgtttc gttcgcttct cagtggataa 4980
cagccagctt ctctgtttaa cagacaaaaa cagcatatcc actcagttcc acatttccat 5040
ataaaggcca aggcatttat tctcaggata attgtttcag catcgcaacc gcatcagact 5100
ccggcatcgc aaactgcacc cggtgccggg cagccacatc cagcgcaaaa accttcgtgt 5160
agacttccgt tgaactgatg gacttatgtc ccatcaggct ttgcagaact ttcagcggta 5220
taccggcata cagcatgtgc atcgcatagg aatggcggaa cgtatgtggt gtgaccggaa 5280
cagagaacgt cacaccgtca gcagcagcgg cggcaaccgc ctccccaatc caggtcctga 5340
ccgttctgtc cgtcacttcc cagatccgcg ctttctctgt ccttcctgtg cgacggttac 5400
gccgctccat gagcttatcg cgaataaata cctgtgacgg aagatcactt cgcagaataa 5460
ataaatcctg gtgtccctgt tgataccggg aagccctggg ccaacttttg gcgaaaatga 5520
gacgttgatc ggcacgtaag aggttccaac tttcaccata atgaaataag atcactaccg 5580
ggcgtatttt ttgagttatc gagattttca ggagctaagg aagctaaaat ggagaaaaaa 5640
atcactggat ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca 5700
tttcagtcag ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt 5760
ttaaagaccg taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc 5820
cgcctgatga atgctcatcc ggaattccgt atggcaatga aagacggtga gctggtgata 5880
tgggatagtg ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg 5940
ctctggagtg aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg 6000
gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc 6060
gtctcagcca atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac 6120
aacttcttcg cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg 6180
atgccgctgg cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg 6240
cttaatgaat tacaacagta ctgcgatgag tggcagggcg gggcgtaatt tttttaaggc 6300
agttattggt gcccttaaac gcctggttgc tacgcctgaa taagtgataa taagcggatg 6360
aatggcagaa attcgatgat aagctgtcaa acatgagaat tggtcgacgg cgcgccaa 6418
<210> 7
<211> 35
<212> DNA
<213> Artificial sequence ()
<400> 7
aaggaaaaaa gcggccgcgt cgacagcgac acact 35
<210> 8
<211> 28
<212> DNA
<213> Artificial sequence ()
<400> 8
ttggcgcgcc gtcgaccaat tctcatgt 28
<210> 9
<211> 1020
<212> DNA
<213> Artificial sequence ()
<400> 9
ttggcgcgcc cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg 60
ggcctttcga cctgcagcct gttgacaatt aatcatcggc atagtatatc ggcatagtat 120
aatacgacaa ggtgaggaac taaaccatgg gatcggccat tgaacaagat ggattgcacg 180
caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa 240
tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg 300
tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt 360
ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa 420
gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc 480
ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg 540
ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg 600
aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg 660
aactgttcgc caggctcaag gcgcgcatgc ccgacggcga tgatctcgtc gtgacccatg 720
gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact 780
gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg 840
ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc 900
ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga ggggatcaat 960
tctctagagc tcgctgatca gcctcgactg tgccttctag ttgcggccgc ttttttcctt 1020
<210> 10
<211> 28
<212> DNA
<213> Artificial sequence ()
<400> 10
ttggcgcgcc cgcttcaaaa gcgcacgt 28
<210> 11
<211> 38
<212> DNA
<213> Artificial sequence ()
<400> 11
aaggaaaaaa gcggccgcaa ctagaaggca cagtcgag 38
<210> 12
<211> 1939
<212> DNA
<213> Artificial sequence ()
<400> 12
ttggcgcgcc cattcaaata tgtatccgct caccgcgatc ctttttaacc catcacatat 60
acctgccgtt cactattatt tagtgaaatg agatattatg atattttctg aattgtgatt 120
aaaaaggcaa ctttatgccc atgcaacaga aactataaaa aatacagaga atgaaaagaa 180
acagatagat tttttagttc tttaggcccg tagtctgcaa atccttttat gattttctat 240
caaacaaaag aggaaaatag accagttgca atccaaacga gagtctaata gaatgaggtc 300
gaaaagtaaa tcgcgcgggt ttgttactga taaagcaggc aagacctaaa atgtgtaaag 360
ggcaaagtgt atactttggc gtcacccctt acatatttta ggtctttttt tattgtgcgt 420
aactaacttg ccatcttcaa acaggagggc tggaagaagc agaccgctaa cacagtacat 480
aaaaaaggag acatgaacga tgaacatcaa aaagtttgca aaacaagcaa cagtattaac 540
ctttactacc gcactgctgg caggaggcgc aactcaagcg tttgcgaaag aaacgaacca 600
aaagccatat aaggaaacat acggcatttc ccatattaca cgccatgata tgctgcaaat 660
ccctgaacag caaaaaaatg aaaaatatca agttcctgaa ttcgattcgt ccacaattaa 720
aaatatctct tctgcaaaag gcctggacgt ttgggacagc tggccattac aaaacgctga 780
cggcactgtc gcaaactatc acggctacca catcgtcttt gcattagccg gagatcctaa 840
aaatgcggat gacacatcga tttacatgtt ctatcaaaaa gtcggcgaaa cttctattga 900
cagctggaaa aacgctggcc gcgtctttaa agacagcgac aaattcgatg caaatgattc 960
tatcctaaaa gaccaaacac aagaatggtc aggttcagcc acatttacat ctgacggaaa 1020
aatccgttta ttctacactg atttctccgg taaacattac ggcaaacaaa cactgacaac 1080
tgcacaagtt aacgtatcag catcagacag ctctttgaac atcaacggtg tagaggatta 1140
taaatcaatc tttgacggtg acggaaaaac gtatcaaaat gtacagcagt tcatcgatga 1200
aggcaactac agctcaggcg acaaccatac gctgagagat cctcactacg tagaagataa 1260
aggccacaaa tacttagtat ttgaagcaaa cactggaact gaagatggct accaaggcga 1320
agaatcttta tttaacaaag catactatgg caaaagcaca tcattcttcc gtcaagaaag 1380
tcaaaaactt ctgcaaagcg ataaaaaacg cacggctgag ttagcaaacg gcgctctcgg 1440
tatgattgag ctaaacgatg attacacact gaaaaaagtg atgaaaccgc tgattgcatc 1500
taacacagta acagatgaaa ttgaacgcgc gaacgtcttt aaaatgaacg gcaaatggta 1560
cctgttcact gactcccgcg gatcaaaaat gacgattgac ggcattacgt ctaacgatat 1620
ttacatgctt ggttatgttt ctaattcttt aactggccca tacaagccgc tgaacaaaac 1680
tggccttgtg ttaaaaatgg atcttgatcc taacgatgta acctttactt actcacactt 1740
cgctgtacct caagcgaaag gaaacaatgt cgtgattaca agctatatga caaacagagg 1800
attctacgca gacaaacaat caacgtttgc gccaagcttc ctgctgaaca tcaaaggcaa 1860
gaaaacatct gttgtcaaag acagcatcct tgaacaagga caattaacag ttaacaaata 1920
agcggccgct tttttcctt 1939
<210> 13
<211> 30
<212> DNA
<213> Artificial sequence ()
<400> 13
ttggcgcgcc cattcaaata tgtatccgct 30
<210> 14
<211> 37
<212> DNA
<213> Artificial sequence ()
<400> 14
aaggaaaaaa gcggccgctt atttgttaac tgttaat 37
<210> 15
<211> 1092
<212> DNA
<213> Artificial sequence ()
<400> 15
ttgtcaaaga cagcatcctt gaacaaggac aattaacagt taacaaataa cgcttcaaaa 60
gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg ggcctttcga cctgcagcct 120
gttgacaatt aatcatcggc atagtatatc ggcatagtat aatacgacaa ggtgaggaac 180
taaaccatgg gatcggccat tgaacaagat ggattgcacg caggttctcc ggccgcttgg 240
gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc 300
gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt 360
gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt 420
ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc 480
gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc 540
atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac 600
caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag 660
gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag 720
gcgcgcatgc ccgacggcga tgatctcgtc gtgacccatg gcgatgcctg cttgccgaat 780
atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg 840
gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa 900
tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc 960
ttctatcgcc ttcttgacga gttcttctga ggggatcaat tctctagagc tcgctgatca 1020
gcctcgactg tgccttctag ttgcggccgc ttttttcctt gtcgacagcg acacacttgc 1080
atcggatgca gc 1092
<210> 16
<211> 72
<212> DNA
<213> Artificial sequence ()
<400> 16
ttgtcaaaga cagcatcctt gaacaaggac aattaacagt taacaaataa cgcttcaaaa 60
gcgcacgtct gc 72
<210> 17
<211> 70
<212> DNA
<213> Artificial sequence ()
<400> 17
gctgcatccg atgcaagtgt gtcgctgtcg acaaggaaaa aagcggccgc aactagaagg 60
cacagtcgag 70
<210> 18
<211> 1066
<212> DNA
<213> Artificial sequence ()
<400> 18
ttgtcaaaga cagcatcctt gaacaaggac aattaacagt taacaaataa cgcggaaccc 60
ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct 120
gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg 180
cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg 240
tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc 300
tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca 360
cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac 420
tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa 480
agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg 540
ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt 600
ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg 660
aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc 720
gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga 780
tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta 840
ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc 900
cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg 960
atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaagcgg 1020
ccgctttttt ccttgtcgac agcgacacac ttgcatcgga tgcagc 1066
<210> 19
<211> 72
<212> DNA
<213> Artificial sequence ()
<400> 19
ttgtcaaaga cagcatcctt gaacaaggac aattaacagt taacaaataa cgcggaaccc 60
ctatttgttt at 72
<210> 20
<211> 69
<212> DNA
<213> Artificial sequence ()
<400> 20
gctgcatccg atgcaagtgt gtcgctgtcg acaaggaaaa aagcggccgc ttaccaatgc 60
ttaatcagt 69
<210> 21
<211> 1572
<212> DNA
<213> New coronavirus ()
<400> 21
gtgaattcga gctcgattaa aggtttatac cttcccaggt aacaaaccaa ccaactttcg 60
atctcttgta gatctgttct ctaaacgaac tttaaaatct gtgtggctgt cactcggctg 120
catgcttagt gcactcacgc agtataatta ataactaatt actgtcgttg acaggacacg 180
agtaactcgt ctatcttctg caggctgctt acggtttcgt ccgtgttgca gccgatcatc 240
agcacatcta ggtttcgtcc gggtgtgacc gaaaggtaag atggagagcc ttgtccctgg 300
tttcaacgag aaaacacacg tccaactcag tttgcctgtt ttacaggttc gcgacgtgct 360
cgtacgtggc tttggagact ccgtggagga ggtcttatca gaggcacgtc aacatcttaa 420
agatggcact tgtggcttag tagaagttga aaaaggcgtt ttgcctcaac ttgaacagcc 480
ctatgtgttc atcaaacgtt cggatgctcg aactgcacct catggtcatg ttatggttga 540
gctggtagca gaactcgaag gcattcagta cggtcgtagc ggtgagacac ttggtgtcct 600
tgtccctcat gtgggcgaaa taccagtggc ttaccgcaag gttcttcttc gtaagaacgg 660
taataaagga gctggtggcc atagttacgg cgccgatcta aagtcatttg acttaggcga 720
cgagcttggc actgatcctt atgaagattt tcaagaaaac tggaacacta aacatagcag 780
tggtgttacc cgtgaactca tgcgtgagct taacggaggg gcatacactc gctatgtcga 840
taacaacttc tgtggccctg atggctaccc tcttgagtgc attaaagacc ttctagcacg 900
tgctggtaaa gcttcatgca ctttgtccga acaactggac tttattgaca ctaagagggg 960
tgtatactgc tgccgtgaac atgagcatga aattgcttgg tacacggaac gttctgaaaa 1020
gagctatgaa ttgcagacac cttttgaaat taaattggca aagaaatttg acaccttcaa 1080
tggggaatgt ccaaattttg tatttccctt aaattccata atcaagacta ttcaaccaag 1140
ggttgaaaag aaaaagcttg atggctttat gggtagaatt cgatctgtct atccagttgc 1200
gtcaccaaat gaatgcaacc aaatgtgcct ttcaactctc atgaagtgtg atcattgtgg 1260
tgaaacttca tggcagacgg gcgattttgt taaagccact tgcgaatttt gtggcactga 1320
gaatttgact aaagaaggtg ccactacttg tggttactta ccccaaaatg ctgttgttaa 1380
aatttattgt ccagcatgtc acaattcaga agtaggacct gagcatagtc ttgccgaata 1440
ccataatgaa tctggcttga aaaccattct tcgtaagggt ggtcgcacta ttgcctttgg 1500
aggctgtgtg ttctcttatg ttggttgcca taacaagtgt gcctattggg ttccacgtgc 1560
tagcgctaac at 1572
<210> 22
<211> 1691
<212> DNA
<213> New coronavirus ()
<400> 22
tgctagcgct aacataggtt gtaaccatac aggtgttgtt ggagaaggtt ccgaaggtct 60
taatgacaac cttcttgaaa tactccaaaa agagaaagtc aacatcaata ttgttggtga 120
ctttaaactt aatgaagaga tcgccattat tttggcatct ttttctgctt ccacaagtgc 180
ttttgtggaa actgtgaaag gtttggatta taaagcattc aaacaaattg ttgaatcctg 240
tggtaatttt aaagttacaa aaggaaaagc taaaaaaggt gcctggaata ttggtgaaca 300
gaaatcaata ctgagtcctc tttatgcatt tgcatcagag gctgctcgtg ttgtacgatc 360
aattttctcc cgcactcttg aaactgctca aaattctgtg cgtgttttac agaaggccgc 420
tataacaata ctagatggaa tttcacagta ttcactgaga ctcattgatg ctatgatgtt 480
cacatctgat ttggctacta acaatctagt tgtaatggcc tacattacag gtggtgttgt 540
tcagttgact tcgcagtggc taactaacat ctttggcact gtttatgaaa aactcaaacc 600
cgtccttgat tggcttgaag agaagtttaa ggaaggtgta gagtttctta gagacggttg 660
ggaaattgtt aaatttatct caacctgtgc ttgtgaaatt gtcggtggac aaattgtcac 720
ctgtgcaaag gaaattaagg agagtgttca gacattcttt aagcttgtaa ataaattttt 780
ggctttgtgt gctgactcta tcattattgg tggagctaaa cttaaagcct tgaatttagg 840
tgaaacattt gtcacgcact caaagggatt gtacagaaag tgtgttaaat ccagagaaga 900
aactggccta ctcatgcctc taaaagcccc aaaagaaatt atcttcttag agggagaaac 960
acttcccaca gaagtgttaa cagaggaagt tgtcttgaaa actggtgatt tacaaccatt 1020
agaacaacct actagtgaag ctgttgaagc tccattggtt ggtacaccag tttgtattaa 1080
cgggcttatg ttgctcgaaa tcaaagacac agaaaagtac tgtgcccttg cacctaatat 1140
gatggtaaca aacaatacct tcacactcaa aggcggtgca ccaacaaagg ttacttttgg 1200
tgatgacact gtgatagaag tgcaaggtta caagagtgtg aatatcactt ttgaacttga 1260
tgaaaggatt gataaagtac ttaatgagaa gtgctctgcc tatacagttg aactcggtac 1320
agaagtaaat gagttcgcct gtgttgtggc agatgctgtc ataaaaactt tgcaaccagt 1380
atctgaatta cttacaccac tgggcattga tttagatgag tggagtatgg ctacatacta 1440
cttatttgat gagtctggtg agtttaaatt ggcttcacat atgtattgtt ctttctaccc 1500
tccagatgag gatgaagaag aaggtgattg tgaagaagaa gagtttgagc catcaactca 1560
atatgagtat ggtactgaag atgattacca aggtaaacct ttggaatttg gtgccacttc 1620
tgctgctctt caacctgaag aagagcaaga agaagattgg ttagatgatg atagtcaaca 1680
aactgttggt c 1691
<210> 23
<211> 1809
<212> DNA
<213> New coronavirus ()
<400> 23
aacaaactgt tggtcaacaa gacggcagtg aggacaatca gacaactact attcaaacaa 60
ttgttgaggt tcaacctcaa ttagagatgg aacttacacc agttgttcag actattgaag 120
tgaatagttt tagtggttat ttaaaactta ctgacaatgt atacattaaa aatgcagaca 180
ttgtggaaga agctaaaaag gtaaaaccaa cagtggttgt taatgcagcc aatgtttacc 240
ttaaacatgg aggaggtgtt gcaggagcct taaataaggc tactaacaat gccatgcaag 300
ttgaatctga tgattacata gctactaatg gaccacttaa agtgggtggt agttgtgttt 360
taagcggaca caatcttgct aaacactgtc ttcatgttgt cggcctaaat gttaacaaag 420
gtgaagacat tcaacttctt aagagtgctt atgaaaattt taatcagcac gaagttctac 480
ttgcaccatt attatcagct ggtatttttg gtgctgaccc tatacattct ttaagagttt 540
gtgtagatac tgttcgcaca aatgtctact tagctgtctt tgataaaaat ctctatgaca 600
aacttgtttc aagctttttg gaaatgaaga gtgaaaagca agttgaacaa aagatcgctg 660
agattcctaa agaggaagtt aagccattta taactgaaag taaaccttca gttgaacaga 720
gaaaacaaga tgataagaaa atcaaagctt gtgttgaaga agttacaaca actctggaag 780
aaactaagtt cctcacagaa aacttgttac tttatattga cattaatggc aatcttcatc 840
cagattctgc cactcttgtt agtgacattg acatcacttt cttaaagaaa gatgctccat 900
atatagtggg tgatgttatt caagagggtg ttttaactgc tgtggttata cctactaaaa 960
aggctggtgg cactactgaa atgctagcga aagctttgag aaaagtgcca acagacaatt 1020
atataaccac ttacccgggt cagggtttaa atggttacac tgtagaggag gcaaagacag 1080
tgcttaaaaa gtgtaaaagt gccttttaca ttctaccatc tattatctct aatgagaagc 1140
aagaaattct tggaactgtt tcttggaatt tgcgagaaat gcttgcacat gcagaagaaa 1200
cacgcaaatt aatgcctgtc tgtgtggaaa ctaaagccat agtttcaact atacagcgta 1260
aatataaggg tattaaaata caagagggtg tggttgatta tggtgctaga ttttactttt 1320
acaccagtaa aacaactgta gcgtcactta tcaacacact taacgatcta aatgaaactc 1380
ttgttacaat gccacttggc tatgtaacac atggcttaaa tttggaagaa gctgctcggt 1440
atatgagatc tctcaaagtg ccagctacag tttctgtttc ttcacctgat gctgttacag 1500
cgtataatgg ttatcttact tcttcttcta aaacacctga agaacatttt attgaaacca 1560
tctcacttgc tggttcctat aaagattggt cctattctgg acaatctaca caactaggta 1620
tagaatttct taagagaggt gataaaagtg tatattacac tagtaatcct accacattcc 1680
acctagatgg tgaagttatc acctttgaca atcttaagac acttctttct ttgagagaag 1740
tgaggactat taaggtgttt acaacagtag acaacattaa cctccacacg caagagcttg 1800
gcgtaatca 1809
<210> 24
<211> 1385
<212> DNA
<213> New coronavirus ()
<400> 24
gtgaattcga gctcgttgtg gacatgtcaa tgacatatgg acaacagttt ggtccaactt 60
atttggatgg agctgatgtt actaaaataa aacctcataa ttcacatgaa ggtaaaacat 120
tttatgtttt acctaatgat gacactctac gtgttgaggc ttttgagtac taccacacaa 180
ctgatcctag ttttctgggt aggtacatgt cagcattaaa tcacactaaa aagtggaaat 240
acccacaagt taatggttta acttctatta aatgggcaga taacaactgt tatcttgcca 300
ctgcattgtt aacactccaa caaatagagt tgaagtttaa tccacctgct ctacaagatg 360
cttattacag agcaagggct ggtgaagctg ctaacttttg tgcacttatc ttagcctact 420
gtaataagac agtaggtgag ttaggtgatg ttagagaaac aatgagttac ttgtttcaac 480
atgccaattt agattcttgc aaaagagtct tgaacgtggt gtgtaaaact tgtggacaac 540
agcagacaac ccttaagggt gtagaagctg ttatgtacat gggcacactt tcttatgaac 600
aatttaagaa aggtgttcag ataccttgta cgtgtggtaa acaagctaca aaatatctag 660
tacaacagga gtcacctttt gttatgatgt cagcaccacc tgctcagtat gaacttaagc 720
atggtacatt tacttgtgct agtgagtaca ctggtaatta ccagtgtggt cactataaac 780
atataacttc taaagaaact ttgtattgca tagacggtgc tttacttaca aagtcctcag 840
aatacaaagg tcctattacg gatgttttct acaaagaaaa cagttacaca acaaccataa 900
aaccagttac ttataaattg gatggtgttg tttgtacaga aattgaccct aagttggaca 960
attattataa gaaagacaat tcttatttca cagagcaacc aattgatctt gtaccaaacc 1020
aaccatatcc aaacgcaagc ttcgataatt ttaagtttgt atgtgataat atcaaatttg 1080
ctgatgattt aaaccagtta actggttata agaaacctgc ttcaagagag cttaaagtta 1140
catttttccc tgacttaaat ggtgatgtgg tggctattga ttataaacac tacacaccct 1200
cttttaagaa aggagctaaa ttgttacata aacctattgt ttggcatgtt aacaatgcaa 1260
ctaataaagc cacgtataaa ccaaatacct ggtgtatacg ttgtctttgg agcacaaaac 1320
cagttgaaac atcaaattcg tttgatgtac tgaagtcaga ggacgcgcag ggaatggata 1380
atctt 1385
<210> 25
<211> 1228
<212> DNA
<213> New coronavirus ()
<400> 25
ggaatggata atcttgcctg cgaagagcta aaaccagtct ctgaagaagt agtggaaaat 60
cctaccatac agaaagacgt tcttgagtgt aatgtgaaaa ctaccgaagt tgtaggagac 120
attatactta aaccagcaaa taatagttta aaaattacag aagaggttgg ccacacagat 180
ctaatggctg cttatgtaga caattctagt cttactatta agaaacctaa tgaattatct 240
agagtattag gtttgaaaac ccttgctact catggtttag ctgctgttaa tagtgtccct 300
tgggatacta tagctaatta tgctaagcct tttcttaaca aagttgttag tacaactact 360
aacatagtta cacggtgttt aaaccgtgtt tgtactaatt atatgcctta tttctttact 420
ttattgctac aattgtgtac ttttactaga agtacaaatt ctagaattaa agcatctatg 480
ccgactacta tagcaaagaa tactgttaag agtgtcggta aattttgtct agaggcttca 540
tttaattatt tgaagtcacc taatttttct aaactgataa atattataat ttggttttta 600
ctattaagtg tttgcctagg ttctttaatc tactcaaccg ctgctttagg tgttttaatg 660
tctaatttag gcatgccttc ttactgtact ggttacagag aaggctattt gaactctact 720
aatgtcacta ttgcaaccta ctgtactggt tctatacctt gtagtgtttg tcttagtggt 780
ttagattctt tagacaccta tccttcttta gaaactatac aaattaccat ttcatctttt 840
aaatgggatt taactgcttt tggcttagtt gcagagtggt ttttggcata tattcttttc 900
actaggtttt tctatgtact tggattggct gcaatcatgc aattgttttt cagctatttt 960
gcagtacatt ttattagtaa ttcttggctt atgtggttaa taattaatct tgtacaaatg 1020
gccccgattt cagctatggt tagaatgtac atcttctttg catcatttta ttatgtatgg 1080
aaaagttatg tgcatgttgt agacggttgt aattcatcaa cttgtatgat gtgttacaaa 1140
cgtaatagag caacaagagt cgaatgtaca actattgtta atggtgttag aaggtccttt 1200
tatgtctatg ctaatggagg taaaggct 1228
<210> 26
<211> 1212
<212> DNA
<213> New coronavirus ()
<400> 26
atggaggtaa aggcttttgc aaactacaca attggaattg tgttaattgt gatacattct 60
gtgctggtag tacatttatt agtgatgaag ttgcgagaga cttgtcacta cagtttaaaa 120
gaccaataaa tcctactgac cagtcttctt acatcgttga tagtgttaca gtgaagaatg 180
gttccatcca tctttacttt gataaagctg gtcaaaagac ttatgaaaga cattctctct 240
ctcattttgt taacttagac aacctgagag ctaataacac taaaggttca ttgcctatta 300
atgttatagt ttttgatggt aaatcaaaat gtgaagaatc atctgcaaaa tcagcgtctg 360
tttactacag tcagcttatg tgtcaaccta tactgttact agatcaggca ttagtgtctg 420
atgttggtga tagtgcggaa gttgcagtta aaatgtttga tgcttacgtt aatacgtttt 480
catcaacttt taacgtacca atggaaaaac tcaaaacact agttgcaact gcagaagctg 540
aacttgcaaa gaatgtgtcc ttagacaatg tcttatctac ttttatttca gcagctcggc 600
aagggtttgt tgattcagat gtagaaacta aagatgttgt tgaatgtctt aaattgtcac 660
atcaatctga catagaagtt actggcgata gttgtaataa ctatatgctc acctataaca 720
aagttgaaaa catgacaccc cgtgaccttg gtgcttgtat tgactgtagt gcgcgtcata 780
ttaatgcgca ggtagcaaaa agtcacaaca ttgctttgat atggaacgtt aaagatttca 840
tgtcattgtc tgaacaacta cgaaaacaaa tacgtagtgc tgctaaaaag aataacttac 900
cttttaagtt gacatgtgca actactagac aagttgttaa tgttgtaaca acaaagatag 960
cacttaaggg tggtaaaatt gttaataatt ggttgaagca gttaattaaa gttacacttg 1020
tgttcctttt tgttgctgct attttctatt taataacacc tgttcatgtc atgtctaaac 1080
atactgactt ttcaagtgaa atcataggat acaaggctat tgatggtggt gtcactcgtg 1140
acatagcatc tacagatact tgttttgcta acaaacatgc tgattttgac acatggttta 1200
gccagcgtgg cg 1212
<210> 27
<211> 1250
<212> DNA
<213> New coronavirus ()
<400> 27
ttagccagcg tggcggtagt tatactaatg acaaagcttg cccattgatt gctgcagtca 60
taacaagaga agtgggtttt gtcgtgcctg gtttgcctgg cacgatatta cgcacaacta 120
atggtgactt tttgcatttc ttacctagag tttttagtgc agttggtaac atctgttaca 180
caccatcaaa acttatagag tacactgact ttgcaacatc agcttgtgtt ttggctgctg 240
aatgtacaat ttttaaagat gcttctggta agccagtacc atattgttat gataccaatg 300
tactagaagg ttctgttgct tatgaaagtt tacgccctga cacacgttat gtgctcatgg 360
atggctctat tattcaattt cctaacacct accttgaagg ttctgttaga gtggtaacaa 420
cttttgattc tgagtactgt aggcacggca cttgtgaaag atcagaagct ggtgtttgtg 480
tatctactag tggtagatgg gtacttaaca atgattatta cagatcttta ccaggagttt 540
tctgtggtgt agatgctgta aatttactta ctaatatgtt tacaccacta attcaaccta 600
ttggtgcttt ggacatatca gcatctatag tagctggtgg tattgtagct atcgtagtaa 660
catgccttgc ctactatttt atgaggttta gaagagcttt tggtgaatac agtcatgtag 720
ttgcctttaa tactttacta ttccttatgt cattcactgt actctgttta acaccagttt 780
actcattctt acctggtgtt tattctgtta tttacttgta cttgacattt tatcttacta 840
atgatgtttc ttttttagca catattcagt ggatggttat gttcacacct ttagtacctt 900
tctggataac aattgcttat atcatttgta tttccacaaa gcatttctat tggttcttta 960
gtaattacct aaagagacgt gtagtcttta atggtgtttc ctttagtact tttgaagaag 1020
ctgcgctgtg cacctttttg ttaaataaag aaatgtatct aaagttgcgt agtgatgtgc 1080
tattacctct tacgcaatat aatagatact tagctcttta taataagtac aagtatttta 1140
gtggagcaat ggatacaact agctacagag aagctgcttg ttgtcatctc gcaaaggctc 1200
tcaatgactt cagtaactca ggttctgatg ttcttagctt ggcgtaatca 1250
<210> 28
<211> 2664
<212> DNA
<213> New coronavirus ()
<400> 28
gtgaattcga gctcgtacca accaccacaa acctctatca cctcagctgt tttgcagagt 60
ggttttagaa aaatggcatt cccatctggt aaagttgagg gttgtatggt acaagtaact 120
tgtggtacaa ctacacttaa cggtctttgg cttgatgacg tagtttactg tccaagacat 180
gtgatctgca cctctgaaga catgcttaac cctaattatg aagatttact cattcgtaag 240
tctaatcata atttcttggt acaggctggt aatgttcaac tcagggttat tggacattct 300
atgcaaaatt gtgtacttaa gcttaaggtt gatacagcca atcctaagac acctaagtat 360
aagtttgttc gcattcaacc aggacagact ttttcagtgt tagcttgtta caatggttca 420
ccatctggtg tttaccaatg tgctatgagg cccaatttca ctattaaggg ttcattcctt 480
aatggttcat gtggtagtgt tggttttaac atagattatg actgtgtctc tttttgttac 540
atgcaccata tggaattacc aactggagtt catgctggca cagacttaga aggtaacttt 600
tatggacctt ttgttgacag gcaaacagca caagcagctg gtacggacac aactattaca 660
gttaatgttt tagcttggtt gtacgctgct gttataaatg gagacaggtg gtttctcaat 720
cgatttacca caactcttaa tgactttaac cttgtggcta tgaagtacaa ttatgaacct 780
ctaacacaag accatgttga catactagga cctctttctg ctcaaactgg aattgccgtt 840
ttagatatgt gtgcttcatt aaaagaatta ctgcaaaatg gtatgaatgg acgtaccata 900
ttgggtagtg ctttattaga agatgaattt acaccttttg atgttgttag acaatgctca 960
ggtgttactt tccaaagtgc agtgaaaaga acaatcaagg gtacacacca ctggttgtta 1020
ctcacaattt tgacttcact tttagtttta gtccagagta ctcaatggtc tttgttcttt 1080
tttttgtatg aaaatgcctt tttacctttt gctatgggta ttattgctat gtctgctttt 1140
gcaatgatgt ttgtcaaaca taagcatgca tttctctgtt tgtttttgtt accttctctt 1200
gccactgtag cttattttaa tatggtctat atgcctgcta gttgggtgat gcgtattatg 1260
acatggttgg atatggttga tactagtttg tctggtttta agctaaaaga ctgtgttatg 1320
tatgcatcag ctgtagtgtt actaatcctt atgacagcaa gaactgtgta tgatgatggt 1380
gctaggagag tgtggacact tatgaatgtc ttgacactcg tttataaagt ttattatggt 1440
aatgctttag atcaagccat ttccatgtgg gctcttataa tctctgttac ttctaactac 1500
tcaggtgtag ttacaactgt catgtttttg gccagaggta ttgtttttat gtgtgttgag 1560
tattgcccta ttttcttcat aactggtaat acacttcagt gtataatgct agtttattgt 1620
ttcttaggct atttttgtac ttgttacttt ggcctctttt gtttactcaa ccgctacttt 1680
agactgactc ttggtgttta tgattactta gtttctacac aggagtttag atatatgaat 1740
tcacagggac tactcccacc caagaatagc atagatgcct tcaaactcaa cattaaattg 1800
ttgggtgttg gtggcaaacc ttgtatcaaa gtagccactg tacagtctaa aatgtcagat 1860
gtaaagtgca cgtcagtagt cttactctca gttttgcaac aactcagagt agaatcatca 1920
tctaaattgt gggctcaatg tgtccagtta cacaatgaca ttctcttagc taaagatact 1980
actgaagcct ttgaaaaaat ggtttcacta ctttctgttt tgctttccat gcagggtgct 2040
gtagacataa acaagctttg tgaagaaatg ctggacaaca gggcaacctt acaagctata 2100
gcctcagagt ttagttccct tccatcatat gcagcttttg ctactgctca agaagcttat 2160
gagcaggctg ttgctaatgg tgattctgaa gttgttctta aaaagttgaa gaagtctttg 2220
aatgtggcta aatctgaatt tgaccgtgat gcagccatgc aacgtaagtt ggaaaagatg 2280
gctgatcaag ctatgaccca aatgtataaa caggctagat ctgaggacaa gagggcaaaa 2340
gttactagtg ctatgcagac aatgcttttc actatgctta gaaagttgga taatgatgca 2400
ctcaacaaca ttatcaacaa tgcaagagat ggttgtgttc ccttgaacat aatacctctt 2460
acaacagcag ccaaactaat ggttgtcata ccagactata acacatataa aaatacgtgt 2520
gatggtacaa catttactta tgcatcagca ttgtgggaaa tccaacaggt tgtagatgca 2580
gatagtaaaa ttgttcaact tagtgaaatt agtatggaca attcacctaa tttagcatgg 2640
cctcttattg taacagcctt aagg 2664
<210> 29
<211> 2381
<212> DNA
<213> New coronavirus ()
<400> 29
gtaacagcct taagggccaa ttctgctgtc aaattacaga ataatgagct tagtcctgtt 60
gcactacgac agatgtcttg tgctgccggt actacacaaa ctgcttgcac tgatgacaat 120
gcgttagctt actacaacac aacaaaggga ggtaggtttg tacttgcact gttatccgat 180
ttacaggatt tgaaatgggc tagattccct aagagtgatg gaactggtac tatctataca 240
gaactggaac caccttgtag gtttgttaca gacacaccta aaggtcctaa agtgaagtat 300
ttatacttta ttaaaggatt aaacaaccta aatagaggta tggtacttgg tagtttagct 360
gccacagtac gtctacaagc tggtaatgca acagaagtgc ctgccaattc aactgtatta 420
tctttctgtg cttttgctgt agatgctgct aaagcttaca aagattatct agctagtggg 480
ggacaaccaa tcactaattg tgttaagatg ttgtgtacat acactggtac tggtcaggca 540
ataacagtta caccggaagc caatatggat caagaatcct ttggtggtgc atcgtgttgt 600
ctgtactgcc gttgccacat agatcatcca aatcctaaag gattttgtga cttaaaaggt 660
aagtatgtac aaatacctac aacttgtgct aatgaccctg tgggttttac acttaaaaac 720
acagtctgta ccgtctgcgg tatgtggaaa ggttatggct gtagttgtga tcaactccgc 780
gaacccatgc ttcagtcagc tgatgcacaa tcgtttttaa acgggtttgc ggtgtaagtg 840
cagcccgtct tacaccgtgc ggcacaggca ctagtactga tgtcgtatac agggcttttg 900
acatctacaa tgataaagta gctggttttg ctaaattcct aaaaactaat tgttgtcgct 960
tccaagaaaa ggacgaagat gacaatttaa ttgattctta ctttgtagtt aagagacaca 1020
ctttctctaa ctaccaacat gaagaaacaa tttataattt acttaaggat tgtccagctg 1080
ttgctaaaca tgacttcttt aagtttagaa tagacggtga catggtacca catatatcac 1140
gtcaacgtct tactaaatac acaatggcag acctcgtcta tgctttaagg cattttgatg 1200
aaggtaattg tgacacatta aaagaaatac ttgtcacata caattgttgt gatgatgatt 1260
atttcaataa aaaggactgg tatgattttg tagaaaaccc agatatatta cgcgtatacg 1320
ccaacttagg tgaacgtgta cgccaagctt tgttaaaaac agtacaattc tgtgatgcca 1380
tgcgaaatgc tggtattgtt ggtgtactga cattagataa tcaagatctc aatggtaact 1440
ggtatgattt cggtgatttc atacaaacca cgccaggtag tggagttcct gttgtagatt 1500
cttattattc attgttaatg cctatattaa ccttgaccag ggctttaact gcagagtcac 1560
atgttgacac tgacttaaca aagccttaca ttaagtggga tttgttaaaa tatgacttca 1620
cggaagagag gttaaaactc tttgaccgtt attttaaata ttgggatcag acataccacc 1680
caaattgtgt taactgtttg gatgacagat gcattctgca ttgtgcaaac tttaatgttt 1740
tattctctac agtgttccca cctacaagtt ttggaccact agtgagaaaa atatttgttg 1800
atggtgttcc atttgtagtt tcaactggat accacttcag agagctaggt gttgtacata 1860
atcaggatgt aaacttacat agctctagac ttagttttaa ggaattactt gtgtatgctg 1920
ctgaccctgc tatgcacgct gcttctggta atctattact agataaacgc actacgtgct 1980
tttcagtagc tgcacttact aacaatgttg cttttcaaac tgtcaaaccc ggtaatttta 2040
acaaagactt ctatgacttt gctgtgtcta agggtttctt taaggaagga agttctgttg 2100
aattaaaaca cttcttcttt gctcaggatg gtaatgctgc tatcagcgat tatgactact 2160
atcgttataa tctaccaaca atgtgtgata tcagacaact actatttgta gttgaagttg 2220
ttgataagta ctttgattgt tacgatggtg gctgtattaa tgctaaccaa gtcatcgtca 2280
acaacctaga caaatcagct ggttttccat ctaataaatg gggtaaggct agactttatt 2340
atgattcaat gagttatgag gatcaaagct tggcgtaatc a 2381
<210> 30
<211> 1593
<212> DNA
<213> New coronavirus ()
<400> 30
gtgaattcga gctcggatgc acttttcgca tatacaaaac gtaatgtcat ccctactata 60
actcaaatga atcttaagta tgccattagt gcaaagaata gagctcgcac cgtagctggt 120
gtctctatct gtagtactat gaccaataga cagtttcatc aaaaattatt gaaatcaata 180
gccgccacta gaggagctac tgtagtaatt ggaacaagca aattctatgg tggttggcac 240
aacatgttaa aaactgttta tagtgatgta gaaaaccctc accttatggg ttgggattat 300
cctaaatgtg atagagccat gcctaacatg cttagaatta tggcctcact tgttcttgct 360
cgcaaacata caacgtgttg tagcttgtca caccgtttct atagattagc taatgagtgt 420
gctcaagtat tgagtgaaat ggtcatgtgt ggcggttcac tatatgttaa accaggtgga 480
acctcatcag gagatgccac aactgcttat gctaatagtg tttttaacat ttgtcaagct 540
gtcacggcca atgttaatgc acttttatct actgatggta acaaaattgc cgataagtat 600
gtccgcaatt tacaacacag actttatgag tgtctctata gaaatagaga tgttgacaca 660
gactttgtga atgagtttta cgcatatttg cgtaaacatt tctcaatgat gatactctct 720
gacgatgctg ttgtgtgttt caatagcact tatgcatctc aaggtctagt ggctagcata 780
aagaacttta agtcagttct ttattatcaa aacaatgttt ttatgtctga agcaaaatgt 840
tggactgaga ctgaccttac taaaggacct catgaatttt gctctcaaca tacaatgcta 900
gttaaacagg gtgatgatta tgtgtacctt ccttacccag atccatcaag aatcctaggg 960
gccggctgtt ttgtagatga tatcgtaaaa acagatggta cacttatgat tgaacggttc 1020
gtgtctttag ctatagatgc ttacccactt actaaacatc ctaatcagga gtatgctgat 1080
gtctttcatt tgtacttaca atacataaga aagctacatg atgagttaac aggacacatg 1140
ttagacatgt attctgttat gcttactaat gataacactt caaggtattg ggaacctgag 1200
ttttatgagg ctatgtacac accgcataca gtcttacagg ctgttggggc ttgtgttctt 1260
tgcaattcac agacttcatt aagatgtggt gcttgcatac gtagaccatt cttatgttgt 1320
aaatgctgtt acgaccatgt catatcaaca tcacataaat tagtcttgtc tgttaatccg 1380
tatgtttgca atgctccagg ttgtgatgtc acagatgtga ctcaacttta cttaggaggt 1440
atgagctatt attgtaaatc acataaacca cccattagtt ttccattgtg tgctaatgga 1500
caagtttttg gtttatataa aaatacatgt gttggtagcg ataatgttac tgactttaat 1560
gcaattgcaa catgtgactg gacaaatgct ggt 1593
<210> 31
<211> 1809
<212> DNA
<213> New coronavirus ()
<400> 31
tggacaaatg ctggtgatta cattttagct aacacctgta ctgaaagact caagcttttt 60
gcagcagaaa cgctcaaagc tactgaggag acatttaaac tgtcttatgg tattgctact 120
gtacgtgaag tgctgtctga cagagaatta catctttcat gggaagttgg taaacctaga 180
ccaccactta accgaaatta tgtctttact ggttatcgtg taactaaaaa cagtaaagta 240
caaataggag agtacacctt tgaaaaaggt gactatggtg atgctgttgt ttaccgaggt 300
acaacaactt acaaattaaa tgttggtgat tattttgtgc tgacatcaca tacagtaatg 360
ccattaagtg cacctacact agtgccacaa gagcactatg ttagaattac tggcttatac 420
ccaacactca atatctcaga tgagttttct agcaatgttg caaattatca aaaggttggt 480
atgcaaaagt attctacact ccagggacca cctggtactg gtaagagtca ttttgctatt 540
ggcctagctc tctactaccc ttctgctcgc atagtgtata cagcttgctc tcatgccgct 600
gttgatgcac tatgtgagaa ggcattaaaa tatttgccta tagataaatg tagtagaatt 660
atacctgcac gtgctcgtgt agagtgtttt gataaattca aagtgaattc aacattagaa 720
cagtatgtct tttgtactgt aaatgtattg cctgagacga cagcagatat agttgtcttt 780
gatgaaattt caatggccac aaattatgat ttgagtgttg tcaatgccag attacgtgct 840
aagcactatg tgtacattgg cgaccctgct caattacctg caccacgcac attgctaact 900
aagggcacac tagaaccaga atatttcaat tcagtgtgta gacttatgaa aactataggt 960
ccagacatgt tcctcggaac ttgtcggcgt tgtcctgctg aaattgttga cactgtgagt 1020
gctttggttt atgataataa gcttaaagca cataaagaca aatcagctca atgctttaaa 1080
atgttttata agggtgttat cacgcatgat gtttcatctg caattaacag gccacaaata 1140
ggcgtggtaa gagaattcct tacacgtaac cctgcttgga gaaaagctgt ctttatttca 1200
ccttataatt cacagaatgc tgtagcctca aagattttgg gactaccaat tcaaactgtt 1260
gattcatcac agggctcaga atatgactat gtcatattca ctcaaaccac tgaaacagct 1320
cactcttgta atgtaaacag atttaatgtt gctattacca gagcaaaagt aggcatactt 1380
tgcataatgt ctgatagaga cctttatgac aagttgcaat ttacaagtct tgaaattcca 1440
cgtaggaatg tggcaacttt acaagctgaa aatgtaacag gactctttaa agattgtagt 1500
aaggtaatca ctgggttaca tcctacacag gcacctacac acctcagtgt tgacactaaa 1560
ttcaaaactg aaggtttatg tgttgacata cctggcatac ctaaggacat gacctataga 1620
agactcatct ctatgatggg ttttaaaatg aattatcaag ttaatggtta ccctaacatg 1680
tttatcaccc gcgaagaagc tataagacat gtacgtgcat ggattggctt cgatgtcgag 1740
gggtgtcatg ctactagaga agctgttggt accaatttac ctttacagct aggtttttct 1800
acaggtgtt 1809
<210> 32
<211> 1658
<212> DNA
<213> New coronavirus ()
<400> 32
ttttctacag gtgttaacct agttgctgta cctacaggtt atgttgatac acctaataat 60
acagatttct ccagagttag tgctaaacca ccgcctggag atcaatttaa acacctcata 120
ccacttatgt acaaaggact tccttggaat gtagtgcgta taaagattgt acaaatgtta 180
agtgacacac ttaaaaatct ctctgacaga gtcgtatttg tcttatgggc acatggcttt 240
gagttgacat ctatgaagta ttttgtgaaa ataggacctg agcgcacctg ttgtctatgt 300
gatagacgtg ccacatgctt ttccactgct tcagacactt atgcctgttg gcatcattct 360
attggatttg attacgtcta taatccgttt atgattgatg ttcaacaatg gggttttaca 420
ggtaacctac aaagcaacca tgatctgtat tgtcaagtcc atggtaatgc acatatagct 480
agttgtgatg caatcatgac taggtgtcta gctgtccacg agtgctttgt taagcgtgtt 540
gactggacta ttgaatatcc tataattggt gatgaactga agattaatgc ggcttgtaga 600
aaggttcaac acatggttgt taaagctgca ttattagcag acaaattccc agttcttcac 660
gacattggta accctaaagc tattaagtgt gtacctcaag ctgatgtaga atggaagttc 720
tatgatgcac agccttgtag tgacaaagct tataaaatag aagaattatt ctattcttat 780
gccacacatt ctgacaaatt cacagatggt gtatgcctat tttggaattg caatgtcgat 840
agatatcctg ctaattccat tgtttgtaga tttgacacta gagtgctatc taaccttaac 900
ttgcctggtt gtgatggtgg cagtttgtat gtaaataaac atgcattcca cacaccagct 960
tttgataaaa gtgcttttgt taatttaaaa caattaccat ttttctatta ctctgacagt 1020
ccatgtgagt ctcatggaaa acaagtagtg tcagatatag attatgtacc actaaagtct 1080
gctacgtgta taacacgttg caatttaggt ggtgctgtct gtagacatca tgctaatgag 1140
tacagattgt atctcgatgc ttataacatg atgatctcag ctggctttag cttgtgggtt 1200
tacaaacaat ttgatactta taacctctgg aacactttta caagacttca gagtttagaa 1260
aatgtggctt ttaatgttgt aaataaggga cactttgatg gacaacaggg tgaagtacca 1320
gtttctatca ttaataacac tgtttacaca aaagttgatg gtgttgatgt agaattgttt 1380
gaaaataaaa caacattacc tgttaatgta gcatttgagc tttgggctaa gcgcaacatt 1440
aaaccagtac cagaggtgaa aatactcaat aatttgggtg tggacattgc tgctaatact 1500
gtgatctggg actacaaaag agatgctcca gcacatatat ctactattgg tgtttgttct 1560
atgactgaca tagccaagaa accaactgaa acgatttgtg caccactcac tgtctttttt 1620
gatggtagag ttgatggtca agtagcttgg cgtaatca 1658
<210> 33
<211> 1653
<212> DNA
<213> New coronavirus ()
<400> 33
gtgaattcga gctcgagact tatttagaaa tgcccgtaat ggtgttctta ttacagaagg 60
tagtgttaaa ggtttacaac catctgtagg tcccaaacaa gctagtctta atggagtcac 120
attaattgga gaagccgtaa aaacacagtt caattattat aagaaagttg atggtgttgt 180
ccaacaatta cctgaaactt actttactca gagtagaaat ttacaagaat ttaaacccag 240
gagtcaaatg gaaattgatt tcttagaatt agctatggat gaattcattg aacggtataa 300
attagaaggc tatgccttcg aacatatcgt ttatggagat tttagtcata gtcagttagg 360
tggtttacat ctactgattg gactagctaa acgttttaag gaatcacctt ttgaattaga 420
agattttatt cctatggaca gtacagttaa aaactatttc ataacagatg cgcaaacagg 480
ttcatctaag tgtgtgtgtt ctgttattga tttattactt gatgattttg ttgaaataat 540
aaaatcccaa gatttatctg tagtttctaa ggttgtcaaa gtgactattg actatacaga 600
aatttcattt atgctttggt gtaaagatgg ccatgtagaa acattttacc caaaattaca 660
atctagtcaa gcgtggcaac cgggtgttgc tatgcctaat ctttacaaaa tgcaaagaat 720
gctattagaa aagtgtgacc ttcaaaatta tggtgatagt gcaacattac ctaaaggcat 780
aatgatgaat gtcgcaaaat atactcaact gtgtcaatat ttaaacacat taacattagc 840
tgtaccctat aatatgagag ttatacattt tggtgctggt tctgataaag gagttgcacc 900
aggtacagct gttttaagac agtggttgcc tacgggtacg ctgcttgtcg attcagatct 960
taatgacttt gtctctgatg cagattcaac tttgattggt gattgtgcaa ctgtacatac 1020
agctaataaa tgggatctca ttattagtga tatgtacgac cctaagacta aaaatgttac 1080
aaaagaaaat gactctaaag agggtttttt cacttacatt tgtgggttta tacaacaaaa 1140
gctagctctt ggaggttccg tggctataaa gataacagaa cattcttgga atgctgatct 1200
ttataagctc atgggacact tcgcatggtg gacagccttt gttactaatg tgaatgcgtc 1260
atcatctgaa gcatttttaa ttggatgtaa ttatcttggc aaaccacgcg aacaaataga 1320
tggttatgtc atgcatgcaa attacatatt ttggaggaat acaaatccaa ttcagttgtc 1380
ttcctattct ttatttgaca tgagtaaatt tccccttaaa ttaaggggta ctgctgttat 1440
gtctttaaaa gaaggtcaaa tcaatgatat gattttatct cttcttagta aaggtagact 1500
tataattaga gaaaacaaca gagttgttat ttctagtgat gttcttgtta acaactaaac 1560
gaacaatgtt tgtttttctt gttttattgc cactagtctc tagtcagtgt gttaatctta 1620
caaccagaac tcaattaccc cctgcataca cta 1653
<210> 34
<211> 1666
<212> DNA
<213> New coronavirus ()
<400> 34
cccctgcata cactaattct ttcacacgtg gtgtttatta ccctgacaaa gttttcagat 60
cctcagtttt acattcaact caggacttgt tcttaccttt cttttccaat gttacttggt 120
tccatgctat acatgtctct gggaccaaag gtactaagag gtttgataac cctgtcctac 180
catttaatga tggtgtttat tttgcttcca ctgagaagtc taacataata agaggctgga 240
tttttggtac tactttagat tcgaagaccc agtccctact tattgttaat aacgctacta 300
atgttgttat taaagtctgt gaatttcaat tttgtaatga tccatttttg ggtgtttatt 360
accacaaaaa caacaaaagt tggatggaaa gtgagttcag agtttattct agtgcgaata 420
attgcacttt tgaatatgtc tctcagcctt ttcttatgga ccttgaagga aaacagggta 480
atttcaaaaa tcttagggaa tttgtgttta agaatattga tggttatttt aaaatatatt 540
ctaagcacac gcctattaat ttagtgcgtg atctccctca gggtttttcg gctttagaac 600
cattggtaga tttgccaata ggtattaaca tcactaggtt tcaaacttta cttgctttac 660
atagaagtta tttgactcct ggtgattctt cttcaggttg gacagctggt gctgcagctt 720
attatgtggg ttatcttcaa cctaggactt ttctattaaa atataatgaa aatggaacca 780
ttacagatgc tgtagactgt gcacttgacc ctctctcaga aacaaagtgt acgttgaaat 840
ccttcactgt agaaaaagga atctatcaaa cttctaactt tagagtccaa ccaacagaat 900
ctattgttag atttcctaat attacaaact tgtgcccttt tggtgaagtt tttaacgcca 960
ccagatttgc atctgtttat gcttggaaca ggaagagaat cagcaactgt gttgctgatt 1020
attctgtcct atataattcc gcatcatttt ccacttttaa gtgttatgga gtgtctccta 1080
ctaaattaaa tgatctctgc tttactaatg tctatgcaga ttcatttgta attagaggtg 1140
atgaagtcag acaaatcgct ccagggcaaa ctggaaagat tgctgattat aattataaat 1200
taccagatga ttttacaggc tgcgttatag cttggaattc taacaatctt gattctaagg 1260
ttggtggtaa ttataattac ctgtatagat tgtttaggaa gtctaatctc aaaccttttg 1320
agagagatat ttcaactgaa atctatcagg ccggtagcac accttgtaat ggtgttgaag 1380
gttttaattg ttactttcct ttacaatcat atggtttcca acccactaat ggtgttggtt 1440
accaaccata cagagtagta gtactttctt ttgaacttct acatgcacca gcaactgttt 1500
gtggacctaa aaagtctact aatttggtta aaaacaaatg tgtcaatttc aacttcaatg 1560
gtttaacagg cacaggtgtt cttactgagt ctaacaaaaa gtttctgcct ttccaacaat 1620
ttggcagaga cattgctgac actactgatg ctgtccgtga tccaca 1666
<210> 35
<211> 1741
<212> DNA
<213> New coronavirus ()
<400> 35
tgtccgtgat ccacagacac ttgagattct tgacattaca ccatgttctt ttggtggtgt 60
cagtgttata acaccaggaa caaatacttc taaccaggtt gctgttcttt atcaggatgt 120
taactgcaca gaagtccctg ttgctattca tgcagatcaa cttactccta cttggcgtgt 180
ttattctaca ggttctaatg tttttcaaac acgtgcaggc tgtttaatag gggctgaata 240
tgtcaacaac tcatatgagt gtgacatacc cattggtgca ggtatatgcg ctagttatca 300
gactcagact aattctcctc ggcgggcacg tagtgtagct agtcaatcca tcattgccta 360
cactatgtca cttggtgcag aaaattcagt tgcttactct aataactcta ttgccatacc 420
cacaaatttt actattagtg ttaccacaga aattctacca gtgtctatga ccaagacatc 480
agtagattgt acaatgtaca tttgtggtga ttcaactgaa tgcagcaatc ttttgttgca 540
atatggcagt ttttgtacac aattaaaccg tgctttaact ggaatagctg ttgaacaaga 600
caaaaacacc caagaagttt ttgcacaagt caaacaaatt tacaaaacac caccaattaa 660
agattttggt ggttttaatt tttcacaaat attaccagat ccatcaaaac caagcaagag 720
gtcatttatt gaagatctac ttttcaacaa agtgacactt gcagatgctg gcttcatcaa 780
acaatatggt gattgccttg gtgatattgc tgctagagac ctcatttgtg cacaaaagtt 840
taacggcctt actgttttgc cacctttgct cacagatgaa atgattgctc aatacacttc 900
tgcactgtta gcgggtacaa tcacttctgg ttggaccttt ggtgcaggtg ctgcattaca 960
aataccattt gctatgcaaa tggcttatag gtttaatggt attggagtta cacagaatgt 1020
tctctatgag aaccaaaaat tgattgccaa ccaatttaat agtgctattg gcaaaattca 1080
agactcactt tcttccacag caagtgcact tggaaaactt caagatgtgg tcaaccaaaa 1140
tgcacaagct ttaaacacgc ttgttaaaca acttagctcc aattttggtg caatttcaag 1200
tgttttaaat gatatccttt cacgtcttga caaagttgag gctgaagtgc aaattgatag 1260
gttgatcaca ggcagacttc aaagtttgca gacatatgtg actcaacaat taattagagc 1320
tgcagaaatc agagcttctg ctaatcttgc tgctactaaa atgtcagagt gtgtacttgg 1380
acaatcaaaa agagttgatt tttgtggaaa gggctatcat cttatgtcct tccctcagtc 1440
agcacctcat ggtgtagtct tcttgcatgt gacttatgtc cctgcacaag aaaagaactt 1500
cacaactgct cctgccattt gtcatgatgg aaaagcacac tttcctcgtg aaggtgtctt 1560
tgtttcaaat ggcacacact ggtttgtaac acaaaggaat ttttatgaac cacaaatcat 1620
tactacagac aacacatttg tgtctggtaa ctgtgatgtt gtaataggaa ttgtcaacaa 1680
cacagtttat gatcctttgc aacctgaatt agactcattc aaggagagct tggcgtaatc 1740
a 1741
<210> 36
<211> 3247
<212> DNA
<213> New coronavirus ()
<400> 36
gtgaattcga gctcggagtt agataaatat tttaagaatc atacatcacc agatgttgat 60
ttaggtgaca tctctggcat taatgcttca gttgtaaaca ttcaaaaaga aattgaccgc 120
ctcaatgagg ttgccaagaa tttaaatgaa tctctcatcg atctccaaga acttggaaag 180
tatgagcagt atataaaatg gccatggtac atttggctag gttttatagc tggcttgatt 240
gccatagtaa tggtgacaat tatgctttgc tgtatgacca gttgctgtag ttgtctcaag 300
ggctgttgtt cttgtggatc ctgctgcaaa tttgatgaag acgactctga gccagtgctc 360
aaaggagtca aattacatta cacataaacg aacttatgga tttgtttatg agaatcttca 420
caattggaac tgtaactttg aagcaaggtg aaatcaagga tgctactcct tcagattttg 480
ttcgcgctac tgcaacgata ccgatacaag cctcactccc tttcggatgg cttattgttg 540
gcgttgcact tcttgctgtt tttcagagcg cttccaaaat cataaccctc aaaaagagat 600
ggcaactagc actctccaag ggtgttcact ttgtttgcaa cttgctgttg ttgtttgtaa 660
cagtttactc acaccttttg ctcgttgctg ctggccttga agcccctttt ctctatcttt 720
atgctttagt ctacttcttg cagagtataa actttgtaag aataataatg aggctttggc 780
tttgctggaa atgccgttcc aaaaacccat tactttatga tgccaactat tttctttgct 840
ggcatactaa ttgttacgac tattgtatac cttacaatag tgtaacttct tcaattgtca 900
ttacttcagg tgatggcaca acaagtccta tttctgaaca tgactaccag attggtggtt 960
atactgaaaa atgggaatct ggagtaaaag actgtgttgt attacacagt tacttcactt 1020
cagactatta ccagctgtac tcaactcaat tgagtacaga cactggtgtt gaacatgtta 1080
ccttcttcat ctacaataaa attgttgatg agcctgaaga acatgtccaa attcacacaa 1140
tcgacggttc atccggagtt gttaatccag taatggaacc aatttatgat gaaccgacga 1200
cgactactag cgtgcctttg taagcacaag ctgatgagta cgaacttatg tactcattcg 1260
ttttggaaga gacaggtacg ttaatagtta atagcgtact tctttttctt gctttcgtgg 1320
tattcttgct agttacacta gccatcctta ctgcgcttcg attgtgtgcg tactgctgca 1380
atattgttaa cgtgagtctt gtaaaacctt ctttttacgt ttactctcgt gttaaaaatc 1440
tgaattcttc tagagttcct gatcttctgg tctaaacgaa ctaaatatta tattagtttt 1500
tctgtttgga actttaattt tagccatggc agattccaac ggtactatta ccgttgaaga 1560
gcttaaaaag ctccttgaac aatggaacct agtaataggt ttcctattcc ttacatggat 1620
ttgtcttcta caatttgcct atgccaacag gaataggttt ttgtatataa ttaagttaat 1680
tttcctctgg ctgttatggc cagtaacttt agcttgtttt gtgcttgctg ctgtttacag 1740
aataaattgg atcaccggtg gaattgctat cgcaatggct tgtcttgtag gcttgatgtg 1800
gctcagctac ttcattgctt ctttcagact gtttgcgcgt acgcgttcca tgtggtcatt 1860
caatccagaa actaacattc ttctcaacgt gccactccat ggcactattc tgaccagacc 1920
gcttctagaa agtgaactcg taatcggagc tgtgatcctt cgtggacatc ttcgtattgc 1980
tggacaccat ctaggacgct gtgacatcaa ggacctgcct aaagaaatca ctgttgctac 2040
atcacgaacg ctttcttatt acaaattggg agcttcgcag cgtgtagcag gtgactcagg 2100
ttttgctgca tacagtcgct acaggattgg caactataaa ttaaacacag accattccag 2160
tagcagtgac aatattgctt tgcttgtaca gtaagtgaca acagatgttt catctcgttg 2220
actttcaggt tactatagca gagatattac taattattat gaggactttt aaagtttcca 2280
tttggaatct tgattacatc ataaacctca taattaaaaa tttatctaag tcactaactg 2340
agaataaata ttctcaatta gatgaagagc aaccaatgga gattgattaa acgaacatga 2400
aaattattct tttcttggca ctgataacac tcgctacttg tgagctttat cactaccaag 2460
agtgtgttag aggtacaaca gtacttttaa aagaaccttg ctcttctgga acatacgagg 2520
gcaattcacc atttcatcct ctagctgata acaaatttgc actgacttgc tttagcactc 2580
aatttgcttt tgcttgtcct gacggcgtaa aacacgtcta tcagttacgt gccagatcag 2640
tttcacctaa actgttcatc agacaagagg aagttcaaga actttactct ccaatttttc 2700
ttattgttgc ggcaatagtg tttataacac tttgcttcac actcaaaaga aagacagaat 2760
gattgaactt tcattaattg acttctattt gtgcttttta gcctttctgc tattccttgt 2820
tttaattatg cttattatct tttggttctc acttgaactg caagatcata atgaaacttg 2880
tcacgcctaa acgaacatga aatttcttgt tttcttagga atcatcacaa ctgtagctgc 2940
atttcaccaa gaatgtagtt tacagtcatg tactcaacat caaccatatg tagttgatga 3000
cccgtgtcct attcacttct attctaaatg gtatattaga gtaggagcta gaaaatcagc 3060
acctttaatt gaattgtgcg tggatgaggc tggttctaaa tcacccattc agtacatcga 3120
tatcggtaat tatacagttt cctgtttacc ttttacaatt aattgccagg aacctaaatt 3180
gggtagtctt gtagtgcgtt gttcgttcta tgaagacttt ttagagtatc atgacgttcg 3240
tgttgtt 3247
<210> 37
<211> 1682
<212> DNA
<213> New coronavirus ()
<400> 37
gacgttcgtg ttgttttaga tttcatctaa acgaacaaac taaaatgtct gataatggac 60
cccaaaatca gcgaaatgca ccccgcatta cgtttggtgg accctcagat tcaactggca 120
gtaaccagaa tggagaacgc agtggggcgc gatcaaaaca acgtcggccc caaggtttac 180
ccaataatac tgcgtcttgg ttcaccgctc tcactcaaca tggcaaggaa gaccttaaat 240
tccctcgagg acaaggcgtt ccaattaaca ccaatagcag tccagatgac caaattggct 300
actaccgaag agctaccaga cgaattcgtg gtggtgacgg taaaatgaaa gatctcagtc 360
caagatggta tttctactac ctaggaactg ggccagaagc tggacttccc tatggtgcta 420
acaaagacgg catcatatgg gttgcaactg agggagcctt gaatacacca aaagatcaca 480
ttggcacccg caatcctgct aacaatgctg caatcgtgct acaacttcct caaggaacaa 540
cattgccaaa aggcttctac gcagaaggga gcagaggcgg cagtcaagcc tcttctcgtt 600
cctcatcacg tagtcgcaac agttcaagaa attcaactcc aggcagcagt aggggaactt 660
ctcctgctag aatggctggc aatggcggtg atgctgctct tgctttgctg ctgcttgaca 720
gattgaacca gcttgagagc aaaatgtctg gtaaaggcca acaacaacaa ggccaaactg 780
tcactaagaa atctgctgct gaggcttcta agaagcctcg gcaaaaacgt actgccacta 840
aagcatacaa tgtaacacaa gctttcggca gacgtggtcc agaacaaacc caaggaaatt 900
ttggggacca ggaactaatc agacaaggaa ctgattacaa acattggccg caaattgcac 960
aatttgcccc cagcgcttca gcgttcttcg gaatgtcgcg cattggcatg gaagtcacac 1020
cttcgggaac gtggttgacc tacacaggtg ccatcaaatt ggatgacaaa gatccaaatt 1080
tcaaagatca agtcattttg ctgaataagc atattgacgc atacaaaaca ttcccaccaa 1140
cagagcctaa aaaggacaaa aagaagaagg ctgatgaaac tcaagcctta ccgcagagac 1200
agaagaaaca gcaaactgtg actcttcttc ctgctgcaga tttggatgat ttctccaaac 1260
aattgcaaca atccatgagc agtgctgact caactcaggc ctaaactcat gcagaccaca 1320
caaggcagat gggctatata aacattttcg cttttccgtt tacgatatat agtctactct 1380
tgtgcagaat gaattctcgt aactacatag cacaagtaga tgtagttaac tttaatctca 1440
catagcaatc tttaatcagt gtgtaacatt agggaggact tgaaagagcc accacatttt 1500
caccgaggcc acgcggagta cgatcgagtg tacagtgaac aatgctaggg agagctgcct 1560
atatggaaga gccctaatgt gtaaaattaa ttttagtagt gctatcccca tgtgatttta 1620
atagcttctt aggagaatga caaaaaaaaa aaaaaaaaaa aaaaaaaagc ttggcgtaat 1680
ca 1682
<210> 38
<211> 34
<212> DNA
<213> Artificial sequence ()
<400> 38
gtgaattcga gctcgattaa aggtttatac cttc 34
<210> 39
<211> 23
<212> DNA
<213> Artificial sequence ()
<400> 39
atgttagcgc tagcacgtgg aac 23
<210> 40
<211> 28
<212> DNA
<213> Artificial sequence ()
<400> 40
tgctagcgct aacataggtt gtaaccat 28
<210> 41
<211> 29
<212> DNA
<213> Artificial sequence ()
<400> 41
gaccaacagt ttgttgacta tcatcatct 29
<210> 42
<211> 29
<212> DNA
<213> Artificial sequence ()
<400> 42
aacaaactgt tggtcaacaa gacggcagt 29
<210> 43
<211> 34
<212> DNA
<213> Artificial sequence ()
<400> 43
tgattacgcc aagctcttgc gtgtggaggt taat 34
<210> 44
<211> 32
<212> DNA
<213> Artificial sequence ()
<400> 44
gtgaattcga gctcgttgtg gacatgtcaa tg 32
<210> 45
<211> 31
<212> DNA
<213> Artificial sequence ()
<400> 45
aagattatcc attccctgcg cgtcctctga c 31
<210> 46
<211> 29
<212> DNA
<213> Artificial sequence ()
<400> 46
ggaatggata atcttgcctg cgaagagct 29
<210> 47
<211> 35
<212> DNA
<213> Artificial sequence ()
<400> 47
agcctttacc tccattagca tagacataaa aggac 35
<210> 48
<211> 34
<212> DNA
<213> Artificial sequence ()
<400> 48
atggaggtaa aggcttttgc aaactacaca attg 34
<210> 49
<211> 30
<212> DNA
<213> Artificial sequence ()
<400> 49
cgccacgctg gctaaaccat gtgtcaaaat 30
<210> 50
<211> 40
<212> DNA
<213> Artificial sequence ()
<400> 50
ttagccagcg tggcggtagt tatactaatg acaaagcttg 40
<210> 51
<211> 35
<212> DNA
<213> Artificial sequence ()
<400> 51
tgattacgcc aagctaagaa catcagaacc tgagt 35
<210> 52
<211> 32
<212> DNA
<213> Artificial sequence ()
<400> 52
gtgaattcga gctcgtacca accaccacaa ac 32
<210> 53
<211> 38
<212> DNA
<213> Artificial sequence ()
<400> 53
ccttaaggct gttacaataa gaggccatgc taaattag 38
<210> 54
<211> 30
<212> DNA
<213> Artificial sequence ()
<400> 54
gtaacagcct taagggccaa ttctgctgtc 30
<210> 55
<211> 31
<212> DNA
<213> Artificial sequence ()
<400> 55
tgattacgcc aagctttgat cctcataact c 31
<210> 56
<211> 31
<212> DNA
<213> Artificial sequence ()
<400> 56
gtgaattcga gctcggatgc acttttcgca t 31
<210> 57
<211> 29
<212> DNA
<213> Artificial sequence ()
<400> 57
accagcattt gtcagtcaca tgttgcaat 29
<210> 58
<211> 30
<212> DNA
<213> Artificial sequence ()
<400> 58
tggacaaatg ctggtgatta cattttagct 30
<210> 59
<211> 31
<212> DNA
<213> Artificial sequence ()
<400> 59
aacacctgta gaaaaaccta gctgtaaagg t 31
<210> 60
<211> 38
<212> DNA
<213> Artificial sequence ()
<400> 60
ttttctacag gtgttaacct agttgctgta cctacagg 38
<210> 61
<211> 33
<212> DNA
<213> Artificial sequence ()
<400> 61
tgattacgcc aagctacttg accatcaact cta 33
<210> 62
<211> 37
<212> DNA
<213> Artificial sequence ()
<400> 62
gtgaattcga gctcgagact tatttagaaa tgcccgt 37
<210> 63
<211> 31
<212> DNA
<213> Artificial sequence ()
<400> 63
tagtgtatgc agggggtaat tgagttctgg t 31
<210> 64
<211> 29
<212> DNA
<213> Artificial sequence ()
<400> 64
cccctgcata cactaattct ttcacacgt 29
<210> 65
<211> 23
<212> DNA
<213> Artificial sequence ()
<400> 65
tgtggatcac ggacagcatc agt 23
<210> 66
<211> 27
<212> DNA
<213> Artificial sequence ()
<400> 66
tgtccgtgat ccacagacac ttgagat 27
<210> 67
<211> 40
<212> DNA
<213> Artificial sequence ()
<400> 67
tgattacgcc aagctctcct tgaatgagtc taattcaggt 40
<210> 68
<211> 39
<212> DNA
<213> Artificial sequence ()
<400> 68
gtgaattcga gctcggagtt agataaatat tttaagaat 39
<210> 69
<211> 25
<212> DNA
<213> Artificial sequence ()
<400> 69
aacaacacga acgtcatgat actct 25
<210> 70
<211> 36
<212> DNA
<213> Artificial sequence ()
<400> 70
gacgttcgtg ttgttttaga tttcatctaa acgaac 36
<210> 71
<211> 53
<212> DNA
<213> Artificial sequence ()
<400> 71
tgattacgcc aagctttttt tttttttttt tttttttttt gtcattctcc tag 53
<210> 72
<211> 4999
<212> DNA
<213> New coronavirus ()
<400> 72
tctcatgttt gacagcttat catcgatagg cgcgccatta aaggtttata ccttcccagg 60
taacaaacca accaactttc gatctcttgt agatctgttc tctaaacgaa ctttaaaatc 120
tgtgtggctg tcactcggct gcatgcttag tgcactcacg cagtataatt aataactaat 180
tactgtcgtt gacaggacac gagtaactcg tctatcttct gcaggctgct tacggtttcg 240
tccgtgttgc agccgatcat cagcacatct aggtttcgtc cgggtgtgac cgaaaggtaa 300
gatggagagc cttgtccctg gtttcaacga gaaaacacac gtccaactca gtttgcctgt 360
tttacaggtt cgcgacgtgc tcgtacgtgg ctttggagac tccgtggagg aggtcttatc 420
agaggcacgt caacatctta aagatggcac ttgtggctta gtagaagttg aaaaaggcgt 480
tttgcctcaa cttgaacagc cctatgtgtt catcaaacgt tcggatgctc gaactgcacc 540
tcatggtcat gttatggttg agctggtagc agaactcgaa ggcattcagt acggtcgtag 600
cggtgagaca cttggtgtcc ttgtccctca tgtgggcgaa ataccagtgg cttaccgcaa 660
ggttcttctt cgtaagaacg gtaataaagg agctggtggc catagttacg gcgccgatct 720
aaagtcattt gacttaggcg acgagcttgg cactgatcct tatgaagatt ttcaagaaaa 780
ctggaacact aaacatagca gtggtgttac ccgtgaactc atgcgtgagc ttaacggagg 840
ggcatacact cgctatgtcg ataacaactt ctgtggccct gatggctacc ctcttgagtg 900
cattaaagac cttctagcac gtgctggtaa agcttcatgc actttgtccg aacaactgga 960
ctttattgac actaagaggg gtgtatactg ctgccgtgaa catgagcatg aaattgcttg 1020
gtacacggaa cgttctgaaa agagctatga attgcagaca ccttttgaaa ttaaattggc 1080
aaagaaattt gacaccttca atggggaatg tccaaatttt gtatttccct taaattccat 1140
aatcaagact attcaaccaa gggttgaaaa gaaaaagctt gatggcttta tgggtagaat 1200
tcgatctgtc tatccagttg cgtcaccaaa tgaatgcaac caaatgtgcc tttcaactct 1260
catgaagtgt gatcattgtg gtgaaacttc atggcagacg ggcgattttg ttaaagccac 1320
ttgcgaattt tgtggcactg agaatttgac taaagaaggt gccactactt gtggttactt 1380
accccaaaat gctgttgtta aaatttattg tccagcatgt cacaattcag aagtaggacc 1440
tgagcatagt cttgccgaat accataatga atctggcttg aaaaccattc ttcgtaaggg 1500
tggtcgcact attgcctttg gaggctgtgt gttctcttat gttggttgcc ataacaagtg 1560
tgcctattgg gttccacgtg ctagcgctaa cataggttgt aaccatacag gtgttgttgg 1620
agaaggttcc gaaggtctta atgacaacct tcttgaaata ctccaaaaag agaaagtcaa 1680
catcaatatt gttggtgact ttaaacttaa tgaagagatc gccattattt tggcatcttt 1740
ttctgcttcc acaagtgctt ttgtggaaac tgtgaaaggt ttggattata aagcattcaa 1800
acaaattgtt gaatcctgtg gtaattttaa agttacaaaa ggaaaagcta aaaaaggtgc 1860
ctggaatatt ggtgaacaga aatcaatact gagtcctctt tatgcatttg catcagaggc 1920
tgctcgtgtt gtacgatcaa ttttctcccg cactcttgaa actgctcaaa attctgtgcg 1980
tgttttacag aaggccgcta taacaatact agatggaatt tcacagtatt cactgagact 2040
cattgatgct atgatgttca catctgattt ggctactaac aatctagttg taatggccta 2100
cattacaggt ggtgttgttc agttgacttc gcagtggcta actaacatct ttggcactgt 2160
ttatgaaaaa ctcaaacccg tccttgattg gcttgaagag aagtttaagg aaggtgtaga 2220
gtttcttaga gacggttggg aaattgttaa atttatctca acctgtgctt gtgaaattgt 2280
cggtggacaa attgtcacct gtgcaaagga aattaaggag agtgttcaga cattctttaa 2340
gcttgtaaat aaatttttgg ctttgtgtgc tgactctatc attattggtg gagctaaact 2400
taaagccttg aatttaggtg aaacatttgt cacgcactca aagggattgt acagaaagtg 2460
tgttaaatcc agagaagaaa ctggcctact catgcctcta aaagccccaa aagaaattat 2520
cttcttagag ggagaaacac ttcccacaga agtgttaaca gaggaagttg tcttgaaaac 2580
tggtgattta caaccattag aacaacctac tagtgaagct gttgaagctc cattggttgg 2640
tacaccagtt tgtattaacg ggcttatgtt gctcgaaatc aaagacacag aaaagtactg 2700
tgcccttgca cctaatatga tggtaacaaa caataccttc acactcaaag gcggtgcacc 2760
aacaaaggtt acttttggtg atgacactgt gatagaagtg caaggttaca agagtgtgaa 2820
tatcactttt gaacttgatg aaaggattga taaagtactt aatgagaagt gctctgccta 2880
tacagttgaa ctcggtacag aagtaaatga gttcgcctgt gttgtggcag atgctgtcat 2940
aaaaactttg caaccagtat ctgaattact tacaccactg ggcattgatt tagatgagtg 3000
gagtatggct acatactact tatttgatga gtctggtgag tttaaattgg cttcacatat 3060
gtattgttct ttctaccctc cagatgagga tgaagaagaa ggtgattgtg aagaagaaga 3120
gtttgagcca tcaactcaat atgagtatgg tactgaagat gattaccaag gtaaaccttt 3180
ggaatttggt gccacttctg ctgctcttca acctgaagaa gagcaagaag aagattggtt 3240
agatgatgat agtcaacaaa ctgttggtca acaagacggc agtgaggaca atcagacaac 3300
tactattcaa acaattgttg aggttcaacc tcaattagag atggaactta caccagttgt 3360
tcagactatt gaagtgaata gttttagtgg ttatttaaaa cttactgaca atgtatacat 3420
taaaaatgca gacattgtgg aagaagctaa aaaggtaaaa ccaacagtgg ttgttaatgc 3480
agccaatgtt taccttaaac atggaggagg tgttgcagga gccttaaata aggctactaa 3540
caatgccatg caagttgaat ctgatgatta catagctact aatggaccac ttaaagtggg 3600
tggtagttgt gttttaagcg gacacaatct tgctaaacac tgtcttcatg ttgtcggcct 3660
aaatgttaac aaaggtgaag acattcaact tcttaagagt gcttatgaaa attttaatca 3720
gcacgaagtt ctacttgcac cattattatc agctggtatt tttggtgctg accctataca 3780
ttctttaaga gtttgtgtag atactgttcg cacaaatgtc tacttagctg tctttgataa 3840
aaatctctat gacaaacttg tttcaagctt tttggaaatg aagagtgaaa agcaagttga 3900
acaaaagatc gctgagattc ctaaagagga agttaagcca tttataactg aaagtaaacc 3960
ttcagttgaa cagagaaaac aagatgataa gaaaatcaaa gcttgtgttg aagaagttac 4020
aacaactctg gaagaaacta agttcctcac agaaaacttg ttactttata ttgacattaa 4080
tggcaatctt catccagatt ctgccactct tgttagtgac attgacatca ctttcttaaa 4140
gaaagatgct ccatatatag tgggtgatgt tattcaagag ggtgttttaa ctgctgtggt 4200
tatacctact aaaaaggctg gtggcactac tgaaatgcta gcgaaagctt tgagaaaagt 4260
gccaacagac aattatataa ccacttaccc gggtcagggt ttaaatggtt acactgtaga 4320
ggaggcaaag acagtgctta aaaagtgtaa aagtgccttt tacattctac catctattat 4380
ctctaatgag aagcaagaaa ttcttggaac tgtttcttgg aatttgcgag aaatgcttgc 4440
acatgcagaa gaaacacgca aattaatgcc tgtctgtgtg gaaactaaag ccatagtttc 4500
aactatacag cgtaaatata agggtattaa aatacaagag ggtgtggttg attatggtgc 4560
tagattttac ttttacacca gtaaaacaac tgtagcgtca cttatcaaca cacttaacga 4620
tctaaatgaa actcttgtta caatgccact tggctatgta acacatggct taaatttgga 4680
agaagctgct cggtatatga gatctctcaa agtgccagct acagtttctg tttcttcacc 4740
tgatgctgtt acagcgtata atggttatct tacttcttct tctaaaacac ctgaagaaca 4800
ttttattgaa accatctcac ttgctggttc ctataaagat tggtcctatt ctggacaatc 4860
tacacaacta ggtatagaat ttcttaagag aggtgataaa agtgtatatt acactagtaa 4920
tcctaccaca ttccacctag atggtgaagt tatcaccttt gacaatctta agacacttct 4980
ttctttgaga gaagtgagg 4999
<210> 73
<211> 5052
<212> DNA
<213> New coronavirus ()
<400> 73
agaagtgagg actattaagg tgtttacaac agtagacaac attaacctcc acacgcaagt 60
tgtggacatg tcaatgacat atggacaaca gtttggtcca acttatttgg atggagctga 120
tgttactaaa ataaaacctc ataattcaca tgaaggtaaa acattttatg ttttacctaa 180
tgatgacact ctacgtgttg aggcttttga gtactaccac acaactgatc ctagttttct 240
gggtaggtac atgtcagcat taaatcacac taaaaagtgg aaatacccac aagttaatgg 300
tttaacttct attaaatggg cagataacaa ctgttatctt gccactgcat tgttaacact 360
ccaacaaata gagttgaagt ttaatccacc tgctctacaa gatgcttatt acagagcaag 420
ggctggtgaa gctgctaact tttgtgcact tatcttagcc tactgtaata agacagtagg 480
tgagttaggt gatgttagag aaacaatgag ttacttgttt caacatgcca atttagattc 540
ttgcaaaaga gtcttgaacg tggtgtgtaa aacttgtgga caacagcaga caacccttaa 600
gggtgtagaa gctgttatgt acatgggcac actttcttat gaacaattta agaaaggtgt 660
tcagatacct tgtacgtgtg gtaaacaagc tacaaaatat ctagtacaac aggagtcacc 720
ttttgttatg atgtcagcac cacctgctca gtatgaactt aagcatggta catttacttg 780
tgctagtgag tacactggta attaccagtg tggtcactat aaacatataa cttctaaaga 840
aactttgtat tgcatagacg gtgctttact tacaaagtcc tcagaataca aaggtcctat 900
tacggatgtt ttctacaaag aaaacagtta cacaacaacc ataaaaccag ttacttataa 960
attggatggt gttgtttgta cagaaattga ccctaagttg gacaattatt ataagaaaga 1020
caattcttat ttcacagagc aaccaattga tcttgtacca aaccaaccat atccaaacgc 1080
aagcttcgat aattttaagt ttgtatgtga taatatcaaa tttgctgatg atttaaacca 1140
gttaactggt tataagaaac ctgcttcaag agagcttaaa gttacatttt tccctgactt 1200
aaatggtgat gtggtggcta ttgattataa acactacaca ccctctttta agaaaggagc 1260
taaattgtta cataaaccta ttgtttggca tgttaacaat gcaactaata aagccacgta 1320
taaaccaaat acctggtgta tacgttgtct ttggagcaca aaaccagttg aaacatcaaa 1380
ttcgtttgat gtactgaagt cagaggacgc gcagggaatg gataatcttg cctgcgaaga 1440
gctaaaacca gtctctgaag aagtagtgga aaatcctacc atacagaaag acgttcttga 1500
gtgtaatgtg aaaactaccg aagttgtagg agacattata cttaaaccag caaataatag 1560
tttaaaaatt acagaagagg ttggccacac agatctaatg gctgcttatg tagacaattc 1620
tagtcttact attaagaaac ctaatgaatt atctagagta ttaggtttga aaacccttgc 1680
tactcatggt ttagctgctg ttaatagtgt cccttgggat actatagcta attatgctaa 1740
gccttttctt aacaaagttg ttagtacaac tactaacata gttacacggt gtttaaaccg 1800
tgtttgtact aattatatgc cttatttctt tactttattg ctacaattgt gtacttttac 1860
tagaagtaca aattctagaa ttaaagcatc tatgccgact actatagcaa agaatactgt 1920
taagagtgtc ggtaaatttt gtctagaggc ttcatttaat tatttgaagt cacctaattt 1980
ttctaaactg ataaatatta taatttggtt tttactatta agtgtttgcc taggttcttt 2040
aatctactca accgctgctt taggtgtttt aatgtctaat ttaggcatgc cttcttactg 2100
tactggttac agagaaggct atttgaactc tactaatgtc actattgcaa cctactgtac 2160
tggttctata ccttgtagtg tttgtcttag tggtttagat tctttagaca cctatccttc 2220
tttagaaact atacaaatta ccatttcatc ttttaaatgg gatttaactg cttttggctt 2280
agttgcagag tggtttttgg catatattct tttcactagg tttttctatg tacttggatt 2340
ggctgcaatc atgcaattgt ttttcagcta ttttgcagta cattttatta gtaattcttg 2400
gcttatgtgg ttaataatta atcttgtaca aatggccccg atttcagcta tggttagaat 2460
gtacatcttc tttgcatcat tttattatgt atggaaaagt tatgtgcatg ttgtagacgg 2520
ttgtaattca tcaacttgta tgatgtgtta caaacgtaat agagcaacaa gagtcgaatg 2580
tacaactatt gttaatggtg ttagaaggtc cttttatgtc tatgctaatg gaggtaaagg 2640
cttttgcaaa ctacacaatt ggaattgtgt taattgtgat acattctgtg ctggtagtac 2700
atttattagt gatgaagttg cgagagactt gtcactacag tttaaaagac caataaatcc 2760
tactgaccag tcttcttaca tcgttgatag tgttacagtg aagaatggtt ccatccatct 2820
ttactttgat aaagctggtc aaaagactta tgaaagacat tctctctctc attttgttaa 2880
cttagacaac ctgagagcta ataacactaa aggttcattg cctattaatg ttatagtttt 2940
tgatggtaaa tcaaaatgtg aagaatcatc tgcaaaatca gcgtctgttt actacagtca 3000
gcttatgtgt caacctatac tgttactaga tcaggcatta gtgtctgatg ttggtgatag 3060
tgcggaagtt gcagttaaaa tgtttgatgc ttacgttaat acgttttcat caacttttaa 3120
cgtaccaatg gaaaaactca aaacactagt tgcaactgca gaagctgaac ttgcaaagaa 3180
tgtgtcctta gacaatgtct tatctacttt tatttcagca gctcggcaag ggtttgttga 3240
ttcagatgta gaaactaaag atgttgttga atgtcttaaa ttgtcacatc aatctgacat 3300
agaagttact ggcgatagtt gtaataacta tatgctcacc tataacaaag ttgaaaacat 3360
gacaccccgt gaccttggtg cttgtattga ctgtagtgcg cgtcatatta atgcgcaggt 3420
agcaaaaagt cacaacattg ctttgatatg gaacgttaaa gatttcatgt cattgtctga 3480
acaactacga aaacaaatac gtagtgctgc taaaaagaat aacttacctt ttaagttgac 3540
atgtgcaact actagacaag ttgttaatgt tgtaacaaca aagatagcac ttaagggtgg 3600
taaaattgtt aataattggt tgaagcagtt aattaaagtt acacttgtgt tcctttttgt 3660
tgctgctatt ttctatttaa taacacctgt tcatgtcatg tctaaacata ctgacttttc 3720
aagtgaaatc ataggataca aggctattga tggtggtgtc actcgtgaca tagcatctac 3780
agatacttgt tttgctaaca aacatgctga ttttgacaca tggtttagcc agcgtggcgg 3840
tagttatact aatgacaaag cttgcccatt gattgctgca gtcataacaa gagaagtggg 3900
ttttgtcgtg cctggtttgc ctggcacgat attacgcaca actaatggtg actttttgca 3960
tttcttacct agagttttta gtgcagttgg taacatctgt tacacaccat caaaacttat 4020
agagtacact gactttgcaa catcagcttg tgttttggct gctgaatgta caatttttaa 4080
agatgcttct ggtaagccag taccatattg ttatgatacc aatgtactag aaggttctgt 4140
tgcttatgaa agtttacgcc ctgacacacg ttatgtgctc atggatggct ctattattca 4200
atttcctaac acctaccttg aaggttctgt tagagtggta acaacttttg attctgagta 4260
ctgtaggcac ggcacttgtg aaagatcaga agctggtgtt tgtgtatcta ctagtggtag 4320
atgggtactt aacaatgatt attacagatc tttaccagga gttttctgtg gtgtagatgc 4380
tgtaaattta cttactaata tgtttacacc actaattcaa cctattggtg ctttggacat 4440
atcagcatct atagtagctg gtggtattgt agctatcgta gtaacatgcc ttgcctacta 4500
ttttatgagg tttagaagag cttttggtga atacagtcat gtagttgcct ttaatacttt 4560
actattcctt atgtcattca ctgtactctg tttaacacca gtttactcat tcttacctgg 4620
tgtttattct gttatttact tgtacttgac attttatctt actaatgatg tttctttttt 4680
agcacatatt cagtggatgg ttatgttcac acctttagta cctttctgga taacaattgc 4740
ttatatcatt tgtatttcca caaagcattt ctattggttc tttagtaatt acctaaagag 4800
acgtgtagtc tttaatggtg tttcctttag tacttttgaa gaagctgcgc tgtgcacctt 4860
tttgttaaat aaagaaatgt atctaaagtt gcgtagtgat gtgctattac ctcttacgca 4920
atataataga tacttagctc tttataataa gtacaagtat tttagtggag caatggatac 4980
aactagctac agagaagctg cttgttgtca tctcgcaaag gcgcggccgc ctaacggatt 5040
caccactcca ag 5052
<210> 74
<211> 5075
<212> DNA
<213> New coronavirus ()
<400> 74
ggatacaact agctacagag aagctgcttg ttgtcatctc gcaaaggcgc tctcaatgac 60
ttcagtaact caggttctga tgttctttac caaccaccac aaacctctat cacctcagct 120
gttttgcaga gtggttttag aaaaatggca ttcccatctg gtaaagttga gggttgtatg 180
gtacaagtaa cttgtggtac aactacactt aacggtcttt ggcttgatga cgtagtttac 240
tgtccaagac atgtgatctg cacctctgaa gacatgctta accctaatta tgaagattta 300
ctcattcgta agtctaatca taatttcttg gtacaggctg gtaatgttca actcagggtt 360
attggacatt ctatgcaaaa ttgtgtactt aagcttaagg ttgatacagc caatcctaag 420
acacctaagt ataagtttgt tcgcattcaa ccaggacaga ctttttcagt gttagcttgt 480
tacaatggtt caccatctgg tgtttaccaa tgtgctatga ggcccaattt cactattaag 540
ggttcattcc ttaatggttc atgtggtagt gttggtttta acatagatta tgactgtgtc 600
tctttttgtt acatgcacca tatggaatta ccaactggag ttcatgctgg cacagactta 660
gaaggtaact tttatggacc ttttgttgac aggcaaacag cacaagcagc tggtacggac 720
acaactatta cagttaatgt tttagcttgg ttgtacgctg ctgttataaa tggagacagg 780
tggtttctca atcgatttac cacaactctt aatgacttta accttgtggc tatgaagtac 840
aattatgaac ctctaacaca agaccatgtt gacatactag gacctctttc tgctcaaact 900
ggaattgccg ttttagatat gtgtgcttca ttaaaagaat tactgcaaaa tggtatgaat 960
ggacgtacca tattgggtag tgctttatta gaagatgaat ttacaccttt tgatgttgtt 1020
agacaatgct caggtgttac tttccaaagt gcagtgaaaa gaacaatcaa gggtacacac 1080
cactggttgt tactcacaat tttgacttca cttttagttt tagtccagag tactcaatgg 1140
tctttgttct tttttttgta tgaaaatgcc tttttacctt ttgctatggg tattattgct 1200
atgtctgctt ttgcaatgat gtttgtcaaa cataagcatg catttctctg tttgtttttg 1260
ttaccttctc ttgccactgt agcttatttt aatatggtct atatgcctgc tagttgggtg 1320
atgcgtatta tgacatggtt ggatatggtt gatactagtt tgtctggttt taagctaaaa 1380
gactgtgtta tgtatgcatc agctgtagtg ttactaatcc ttatgacagc aagaactgtg 1440
tatgatgatg gtgctaggag agtgtggaca cttatgaatg tcttgacact cgtttataaa 1500
gtttattatg gtaatgcttt agatcaagcc atttccatgt gggctcttat aatctctgtt 1560
acttctaact actcaggtgt agttacaact gtcatgtttt tggccagagg tattgttttt 1620
atgtgtgttg agtattgccc tattttcttc ataactggta atacacttca gtgtataatg 1680
ctagtttatt gtttcttagg ctatttttgt acttgttact ttggcctctt ttgtttactc 1740
aaccgctact ttagactgac tcttggtgtt tatgattact tagtttctac acaggagttt 1800
agatatatga attcacaggg actactccca cccaagaata gcatagatgc cttcaaactc 1860
aacattaaat tgttgggtgt tggtggcaaa ccttgtatca aagtagccac tgtacagtct 1920
aaaatgtcag atgtaaagtg cacgtcagta gtcttactct cagttttgca acaactcaga 1980
gtagaatcat catctaaatt gtgggctcaa tgtgtccagt tacacaatga cattctctta 2040
gctaaagata ctactgaagc ctttgaaaaa atggtttcac tactttctgt tttgctttcc 2100
atgcagggtg ctgtagacat aaacaagctt tgtgaagaaa tgctggacaa cagggcaacc 2160
ttacaagcta tagcctcaga gtttagttcc cttccatcat atgcagcttt tgctactgct 2220
caagaagctt atgagcaggc tgttgctaat ggtgattctg aagttgttct taaaaagttg 2280
aagaagtctt tgaatgtggc taaatctgaa tttgaccgtg atgcagccat gcaacgtaag 2340
ttggaaaaga tggctgatca agctatgacc caaatgtata aacaggctag atctgaggac 2400
aagagggcaa aagttactag tgctatgcag acaatgcttt tcactatgct tagaaagttg 2460
gataatgatg cactcaacaa cattatcaac aatgcaagag atggttgtgt tcccttgaac 2520
ataatacctc ttacaacagc agccaaacta atggttgtca taccagacta taacacatat 2580
aaaaatacgt gtgatggtac aacatttact tatgcatcag cattgtggga aatccaacag 2640
gttgtagatg cagatagtaa aattgttcaa cttagtgaaa ttagtatgga caattcacct 2700
aatttagcat ggcctcttat tgtaacagcc ttaagggcca attctgctgt caaattacag 2760
aataatgagc ttagtcctgt tgcactacga cagatgtctt gtgctgccgg tactacacaa 2820
actgcttgca ctgatgacaa tgcgttagct tactacaaca caacaaaggg aggtaggttt 2880
gtacttgcac tgttatccga tttacaggat ttgaaatggg ctagattccc taagagtgat 2940
ggaactggta ctatctatac agaactggaa ccaccttgta ggtttgttac agacacacct 3000
aaaggtccta aagtgaagta tttatacttt attaaaggat taaacaacct aaatagaggt 3060
atggtacttg gtagtttagc tgccacagta cgtctacaag ctggtaatgc aacagaagtg 3120
cctgccaatt caactgtatt atctttctgt gcttttgctg tagatgctgc taaagcttac 3180
aaagattatc tagctagtgg gggacaacca atcactaatt gtgttaagat gttgtgtaca 3240
tacactggta ctggtcaggc aataacagtt acaccggaag ccaatatgga tcaagaatcc 3300
tttggtggtg catcgtgttg tctgtactgc cgttgccaca tagatcatcc aaatcctaaa 3360
ggattttgtg acttaaaagg taagtatgta caaataccta caacttgtgc taatgaccct 3420
gtgggtttta cacttaaaaa cacagtctgt accgtctgcg gtatgtggaa aggttatggc 3480
tgtagttgtg atcaactccg cgaacccatg cttcagtcag ctgatgcaca atcgttttta 3540
aacgggtttg cggtgtaagt gcagcccgtc ttacaccgtg cggcacaggc actagtactg 3600
atgtcgtata cagggctttt gacatctaca atgataaagt agctggtttt gctaaattcc 3660
taaaaactaa ttgttgtcgc ttccaagaaa aggacgaaga tgacaattta attgattctt 3720
actttgtagt taagagacac actttctcta actaccaaca tgaagaaaca atttataatt 3780
tacttaagga ttgtccagct gttgctaaac atgacttctt taagtttaga atagacggtg 3840
acatggtacc acatatatca cgtcaacgtc ttactaaata cacaatggca gacctcgtct 3900
atgctttaag gcattttgat gaaggtaatt gtgacacatt aaaagaaata cttgtcacat 3960
acaattgttg tgatgatgat tatttcaata aaaaggactg gtatgatttt gtagaaaacc 4020
cagatatatt acgcgtatac gccaacttag gtgaacgtgt acgccaagct ttgttaaaaa 4080
cagtacaatt ctgtgatgcc atgcgaaatg ctggtattgt tggtgtactg acattagata 4140
atcaagatct caatggtaac tggtatgatt tcggtgattt catacaaacc acgccaggta 4200
gtggagttcc tgttgtagat tcttattatt cattgttaat gcctatatta accttgacca 4260
gggctttaac tgcagagtca catgttgaca ctgacttaac aaagccttac attaagtggg 4320
atttgttaaa atatgacttc acggaagaga ggttaaaact ctttgaccgt tattttaaat 4380
attgggatca gacataccac ccaaattgtg ttaactgttt ggatgacaga tgcattctgc 4440
attgtgcaaa ctttaatgtt ttattctcta cagtgttccc acctacaagt tttggaccac 4500
tagtgagaaa aatatttgtt gatggtgttc catttgtagt ttcaactgga taccacttca 4560
gagagctagg tgttgtacat aatcaggatg taaacttaca tagctctaga cttagtttta 4620
aggaattact tgtgtatgct gctgaccctg ctatgcacgc tgcttctggt aatctattac 4680
tagataaacg cactacgtgc ttttcagtag ctgcacttac taacaatgtt gcttttcaaa 4740
ctgtcaaacc cggtaatttt aacaaagact tctatgactt tgctgtgtct aagggtttct 4800
ttaaggaagg aagttctgtt gaattaaaac acttcttctt tgctcaggat ggtaatgctg 4860
ctatcagcga ttatgactac tatcgttata atctaccaac aatgtgtgat atcagacaac 4920
tactatttgt agttgaagtt gttgataagt actttgattg ttacgatggt ggctgtatta 4980
atgctaacca agtcatcgtc aacaacctag acaaatcagc tggttttcca tctaataaat 5040
ggggtaaggc tagactttat tatgattcaa tgagt 5075
<210> 75
<211> 5081
<212> DNA
<213> New coronavirus ()
<400> 75
ttccatctaa taaatggggt aaggctagac tttattatga ttcaatgagt tatgaggatc 60
aagatgcact tttcgcatat acaaaacgta atgtcatccc tactataact caaatgaatc 120
ttaagtatgc cattagtgca aagaatagag ctcgcaccgt agctggtgtc tctatctgta 180
gtactatgac caatagacag tttcatcaaa aattattgaa atcaatagcc gccactagag 240
gagctactgt agtaattgga acaagcaaat tctatggtgg ttggcacaac atgttaaaaa 300
ctgtttatag tgatgtagaa aaccctcacc ttatgggttg ggattatcct aaatgtgata 360
gagccatgcc taacatgctt agaattatgg cctcacttgt tcttgctcgc aaacatacaa 420
cgtgttgtag cttgtcacac cgtttctata gattagctaa tgagtgtgct caagtattga 480
gtgaaatggt catgtgtggc ggttcactat atgttaaacc aggtggaacc tcatcaggag 540
atgccacaac tgcttatgct aatagtgttt ttaacatttg tcaagctgtc acggccaatg 600
ttaatgcact tttatctact gatggtaaca aaattgccga taagtatgtc cgcaatttac 660
aacacagact ttatgagtgt ctctatagaa atagagatgt tgacacagac tttgtgaatg 720
agttttacgc atatttgcgt aaacatttct caatgatgat actctctgac gatgctgttg 780
tgtgtttcaa tagcacttat gcatctcaag gtctagtggc tagcataaag aactttaagt 840
cagttcttta ttatcaaaac aatgttttta tgtctgaagc aaaatgttgg actgagactg 900
accttactaa aggacctcat gaattttgct ctcaacatac aatgctagtt aaacagggtg 960
atgattatgt gtaccttcct tacccagatc catcaagaat cctaggggcc ggctgttttg 1020
tagatgatat cgtaaaaaca gatggtacac ttatgattga acggttcgtg tctttagcta 1080
tagatgctta cccacttact aaacatccta atcaggagta tgctgatgtc tttcatttgt 1140
acttacaata cataagaaag ctacatgatg agttaacagg acacatgtta gacatgtatt 1200
ctgttatgct tactaatgat aacacttcaa ggtattggga acctgagttt tatgaggcta 1260
tgtacacacc gcatacagtc ttacaggctg ttggggcttg tgttctttgc aattcacaga 1320
cttcattaag atgtggtgct tgcatacgta gaccattctt atgttgtaaa tgctgttacg 1380
accatgtcat atcaacatca cataaattag tcttgtctgt taatccgtat gtttgcaatg 1440
ctccaggttg tgatgtcaca gatgtgactc aactttactt aggaggtatg agctattatt 1500
gtaaatcaca taaaccaccc attagttttc cattgtgtgc taatggacaa gtttttggtt 1560
tatataaaaa tacatgtgtt ggtagcgata atgttactga ctttaatgca attgcaacat 1620
gtgactggac aaatgctggt gattacattt tagctaacac ctgtactgaa agactcaagc 1680
tttttgcagc agaaacgctc aaagctactg aggagacatt taaactgtct tatggtattg 1740
ctactgtacg tgaagtgctg tctgacagag aattacatct ttcatgggaa gttggtaaac 1800
ctagaccacc acttaaccga aattatgtct ttactggtta tcgtgtaact aaaaacagta 1860
aagtacaaat aggagagtac acctttgaaa aaggtgacta tggtgatgct gttgtttacc 1920
gaggtacaac aacttacaaa ttaaatgttg gtgattattt tgtgctgaca tcacatacag 1980
taatgccatt aagtgcacct acactagtgc cacaagagca ctatgttaga attactggct 2040
tatacccaac actcaatatc tcagatgagt tttctagcaa tgttgcaaat tatcaaaagg 2100
ttggtatgca aaagtattct acactccagg gaccacctgg tactggtaag agtcattttg 2160
ctattggcct agctctctac tacccttctg ctcgcatagt gtatacagct tgctctcatg 2220
ccgctgttga tgcactatgt gagaaggcat taaaatattt gcctatagat aaatgtagta 2280
gaattatacc tgcacgtgct cgtgtagagt gttttgataa attcaaagtg aattcaacat 2340
tagaacagta tgtcttttgt actgtaaatg tattgcctga gacgacagca gatatagttg 2400
tctttgatga aatttcaatg gccacaaatt atgatttgag tgttgtcaat gccagattac 2460
gtgctaagca ctatgtgtac attggcgacc ctgctcaatt acctgcacca cgcacattgc 2520
taactaaggg cacactagaa ccagaatatt tcaattcagt gtgtagactt atgaaaacta 2580
taggtccaga catgttcctc ggaacttgtc ggcgttgtcc tgctgaaatt gttgacactg 2640
tgagtgcttt ggtttatgat aataagctta aagcacataa agacaaatca gctcaatgct 2700
ttaaaatgtt ttataagggt gttatcacgc atgatgtttc atctgcaatt aacaggccac 2760
aaataggcgt ggtaagagaa ttccttacac gtaaccctgc ttggagaaaa gctgtcttta 2820
tttcacctta taattcacag aatgctgtag cctcaaagat tttgggacta ccaattcaaa 2880
ctgttgattc atcacagggc tcagaatatg actatgtcat attcactcaa accactgaaa 2940
cagctcactc ttgtaatgta aacagattta atgttgctat taccagagca aaagtaggca 3000
tactttgcat aatgtctgat agagaccttt atgacaagtt gcaatttaca agtcttgaaa 3060
ttccacgtag gaatgtggca actttacaag ctgaaaatgt aacaggactc tttaaagatt 3120
gtagtaaggt aatcactggg ttacatccta cacaggcacc tacacacctc agtgttgaca 3180
ctaaattcaa aactgaaggt ttatgtgttg acatacctgg catacctaag gacatgacct 3240
atagaagact catctctatg atgggtttta aaatgaatta tcaagttaat ggttacccta 3300
acatgtttat cacccgcgaa gaagctataa gacatgtacg tgcatggatt ggcttcgatg 3360
tcgaggggtg tcatgctact agagaagctg ttggtaccaa tttaccttta cagctaggtt 3420
tttctacagg tgttaaccta gttgctgtac ctacaggtta tgttgataca cctaataata 3480
cagatttctc cagagttagt gctaaaccac cgcctggaga tcaatttaaa cacctcatac 3540
cacttatgta caaaggactt ccttggaatg tagtgcgtat aaagattgta caaatgttaa 3600
gtgacacact taaaaatctc tctgacagag tcgtatttgt cttatgggca catggctttg 3660
agttgacatc tatgaagtat tttgtgaaaa taggacctga gcgcacctgt tgtctatgtg 3720
atagacgtgc cacatgcttt tccactgctt cagacactta tgcctgttgg catcattcta 3780
ttggatttga ttacgtctat aatccgttta tgattgatgt tcaacaatgg ggttttacag 3840
gtaacctaca aagcaaccat gatctgtatt gtcaagtcca tggtaatgca catatagcta 3900
gttgtgatgc aatcatgact aggtgtctag ctgtccacga gtgctttgtt aagcgtgttg 3960
actggactat tgaatatcct ataattggtg atgaactgaa gattaatgcg gcttgtagaa 4020
aggttcaaca catggttgtt aaagctgcat tattagcaga caaattccca gttcttcacg 4080
acattggtaa ccctaaagct attaagtgtg tacctcaagc tgatgtagaa tggaagttct 4140
atgatgcaca gccttgtagt gacaaagctt ataaaataga agaattattc tattcttatg 4200
ccacacattc tgacaaattc acagatggtg tatgcctatt ttggaattgc aatgtcgata 4260
gatatcctgc taattccatt gtttgtagat ttgacactag agtgctatct aaccttaact 4320
tgcctggttg tgatggtggc agtttgtatg taaataaaca tgcattccac acaccagctt 4380
ttgataaaag tgcttttgtt aatttaaaac aattaccatt tttctattac tctgacagtc 4440
catgtgagtc tcatggaaaa caagtagtgt cagatataga ttatgtacca ctaaagtctg 4500
ctacgtgtat aacacgttgc aatttaggtg gtgctgtctg tagacatcat gctaatgagt 4560
acagattgta tctcgatgct tataacatga tgatctcagc tggctttagc ttgtgggttt 4620
acaaacaatt tgatacttat aacctctgga acacttttac aagacttcag agtttagaaa 4680
atgtggcttt taatgttgta aataagggac actttgatgg acaacagggt gaagtaccag 4740
tttctatcat taataacact gtttacacaa aagttgatgg tgttgatgta gaattgtttg 4800
aaaataaaac aacattacct gttaatgtag catttgagct ttgggctaag cgcaacatta 4860
aaccagtacc agaggtgaaa atactcaata atttgggtgt ggacattgct gctaatactg 4920
tgatctggga ctacaaaaga gatgctccag cacatatatc tactattggt gtttgttcta 4980
tgactgacat agccaagaaa ccaactgaaa cgatttgtgc accacgcggc cgcctaacgg 5040
attcaccact ccaagaattg gagccaatca attcttgcgg a 5081
<210> 76
<211> 5075
<212> DNA
<213> New coronavirus ()
<400> 76
ctatgactga catagccaag aaaccaactg aaacgatttg tgcaccacgc tcactgtctt 60
ttttgatggt agagttgatg gtcaagtaga cttatttaga aatgcccgta atggtgttct 120
tattacagaa ggtagtgtta aaggtttaca accatctgta ggtcccaaac aagctagtct 180
taatggagtc acattaattg gagaagccgt aaaaacacag ttcaattatt ataagaaagt 240
tgatggtgtt gtccaacaat tacctgaaac ttactttact cagagtagaa atttacaaga 300
atttaaaccc aggagtcaaa tggaaattga tttcttagaa ttagctatgg atgaattcat 360
tgaacggtat aaattagaag gctatgcctt cgaacatatc gtttatggag attttagtca 420
tagtcagtta ggtggtttac atctactgat tggactagct aaacgtttta aggaatcacc 480
ttttgaatta gaagatttta ttcctatgga cagtacagtt aaaaactatt tcataacaga 540
tgcgcaaaca ggttcatcta agtgtgtgtg ttctgttatt gatttattac ttgatgattt 600
tgttgaaata ataaaatccc aagatttatc tgtagtttct aaggttgtca aagtgactat 660
tgactataca gaaatttcat ttatgctttg gtgtaaagat ggccatgtag aaacatttta 720
cccaaaatta caatctagtc aagcgtggca accgggtgtt gctatgccta atctttacaa 780
aatgcaaaga atgctattag aaaagtgtga ccttcaaaat tatggtgata gtgcaacatt 840
acctaaaggc ataatgatga atgtcgcaaa atatactcaa ctgtgtcaat atttaaacac 900
attaacatta gctgtaccct ataatatgag agttatacat tttggtgctg gttctgataa 960
aggagttgca ccaggtacag ctgttttaag acagtggttg cctacgggta cgctgcttgt 1020
cgattcagat cttaatgact ttgtctctga tgcagattca actttgattg gtgattgtgc 1080
aactgtacat acagctaata aatgggatct cattattagt gatatgtacg accctaagac 1140
taaaaatgtt acaaaagaaa atgactctaa agagggtttt ttcacttaca tttgtgggtt 1200
tatacaacaa aagctagctc ttggaggttc cgtggctata aagataacag aacattcttg 1260
gaatgctgat ctttataagc tcatgggaca cttcgcatgg tggacagcct ttgttactaa 1320
tgtgaatgcg tcatcatctg aagcattttt aattggatgt aattatcttg gcaaaccacg 1380
cgaacaaata gatggttatg tcatgcatgc aaattacata ttttggagga atacaaatcc 1440
aattcagttg tcttcctatt ctttatttga catgagtaaa tttcccctta aattaagggg 1500
tactgctgtt atgtctttaa aagaaggtca aatcaatgat atgattttat ctcttcttag 1560
taaaggtaga cttataatta gagaaaacaa cagagttgtt atttctagtg atgttcttgt 1620
taacaactaa acgaacaatg tttgtttttc ttgttttatt gccactagtc tctagtcagt 1680
gtgttaatct tacaaccaga actcaattac cccctgcata cactaattct ttcacacgtg 1740
gtgtttatta ccctgacaaa gttttcagat cctcagtttt acattcaact caggacttgt 1800
tcttaccttt cttttccaat gttacttggt tccatgctat acatgtctct gggaccaaag 1860
gtactaagag gtttgataac cctgtcctac catttaatga tggtgtttat tttgcttcca 1920
ctgagaagtc taacataata agaggctgga tttttggtac tactttagat tcgaagaccc 1980
agtccctact tattgttaat aacgctacta atgttgttat taaagtctgt gaatttcaat 2040
tttgtaatga tccatttttg ggtgtttatt accacaaaaa caacaaaagt tggatggaaa 2100
gtgagttcag agtttattct agtgcgaata attgcacttt tgaatatgtc tctcagcctt 2160
ttcttatgga ccttgaagga aaacagggta atttcaaaaa tcttagggaa tttgtgttta 2220
agaatattga tggttatttt aaaatatatt ctaagcacac gcctattaat ttagtgcgtg 2280
atctccctca gggtttttcg gctttagaac cattggtaga tttgccaata ggtattaaca 2340
tcactaggtt tcaaacttta cttgctttac atagaagtta tttgactcct ggtgattctt 2400
cttcaggttg gacagctggt gctgcagctt attatgtggg ttatcttcaa cctaggactt 2460
ttctattaaa atataatgaa aatggaacca ttacagatgc tgtagactgt gcacttgacc 2520
ctctctcaga aacaaagtgt acgttgaaat ccttcactgt agaaaaagga atctatcaaa 2580
cttctaactt tagagtccaa ccaacagaat ctattgttag atttcctaat attacaaact 2640
tgtgcccttt tggtgaagtt tttaacgcca ccagatttgc atctgtttat gcttggaaca 2700
ggaagagaat cagcaactgt gttgctgatt attctgtcct atataattcc gcatcatttt 2760
ccacttttaa gtgttatgga gtgtctccta ctaaattaaa tgatctctgc tttactaatg 2820
tctatgcaga ttcatttgta attagaggtg atgaagtcag acaaatcgct ccagggcaaa 2880
ctggaaagat tgctgattat aattataaat taccagatga ttttacaggc tgcgttatag 2940
cttggaattc taacaatctt gattctaagg ttggtggtaa ttataattac ctgtatagat 3000
tgtttaggaa gtctaatctc aaaccttttg agagagatat ttcaactgaa atctatcagg 3060
ccggtagcac accttgtaat ggtgttgaag gttttaattg ttactttcct ttacaatcat 3120
atggtttcca acccactaat ggtgttggtt accaaccata cagagtagta gtactttctt 3180
ttgaacttct acatgcacca gcaactgttt gtggacctaa aaagtctact aatttggtta 3240
aaaacaaatg tgtcaatttc aacttcaatg gtttaacagg cacaggtgtt cttactgagt 3300
ctaacaaaaa gtttctgcct ttccaacaat ttggcagaga cattgctgac actactgatg 3360
ctgtccgtga tccacagaca cttgagattc ttgacattac accatgttct tttggtggtg 3420
tcagtgttat aacaccagga acaaatactt ctaaccaggt tgctgttctt tatcaggatg 3480
ttaactgcac agaagtccct gttgctattc atgcagatca acttactcct acttggcgtg 3540
tttattctac aggttctaat gtttttcaaa cacgtgcagg ctgtttaata ggggctgaat 3600
atgtcaacaa ctcatatgag tgtgacatac ccattggtgc aggtatatgc gctagttatc 3660
agactcagac taattctcct cggcgggcac gtagtgtagc tagtcaatcc atcattgcct 3720
acactatgtc acttggtgca gaaaattcag ttgcttactc taataactct attgccatac 3780
ccacaaattt tactattagt gttaccacag aaattctacc agtgtctatg accaagacat 3840
cagtagattg tacaatgtac atttgtggtg attcaactga atgcagcaat cttttgttgc 3900
aatatggcag tttttgtaca caattaaacc gtgctttaac tggaatagct gttgaacaag 3960
acaaaaacac ccaagaagtt tttgcacaag tcaaacaaat ttacaaaaca ccaccaatta 4020
aagattttgg tggttttaat ttttcacaaa tattaccaga tccatcaaaa ccaagcaaga 4080
ggtcatttat tgaagatcta cttttcaaca aagtgacact tgcagatgct ggcttcatca 4140
aacaatatgg tgattgcctt ggtgatattg ctgctagaga cctcatttgt gcacaaaagt 4200
ttaacggcct tactgttttg ccacctttgc tcacagatga aatgattgct caatacactt 4260
ctgcactgtt agcgggtaca atcacttctg gttggacctt tggtgcaggt gctgcattac 4320
aaataccatt tgctatgcaa atggcttata ggtttaatgg tattggagtt acacagaatg 4380
ttctctatga gaaccaaaaa ttgattgcca accaatttaa tagtgctatt ggcaaaattc 4440
aagactcact ttcttccaca gcaagtgcac ttggaaaact tcaagatgtg gtcaaccaaa 4500
atgcacaagc tttaaacacg cttgttaaac aacttagctc caattttggt gcaatttcaa 4560
gtgttttaaa tgatatcctt tcacgtcttg acaaagttga ggctgaagtg caaattgata 4620
ggttgatcac aggcagactt caaagtttgc agacatatgt gactcaacaa ttaattagag 4680
ctgcagaaat cagagcttct gctaatcttg ctgctactaa aatgtcagag tgtgtacttg 4740
gacaatcaaa aagagttgat ttttgtggaa agggctatca tcttatgtcc ttccctcagt 4800
cagcacctca tggtgtagtc ttcttgcatg tgacttatgt ccctgcacaa gaaaagaact 4860
tcacaactgc tcctgccatt tgtcatgatg gaaaagcaca ctttcctcgt gaaggtgtct 4920
ttgtttcaaa tggcacacac tggtttgtaa cacaaaggaa tttttatgaa ccacaaatca 4980
ttactacaga caacacattt gtgtctggta actgtgatgt tgtaatagga attgtcaaca 5040
acacagttta tgatcctttg caacctgaat tagac 5075
<210> 77
<211> 4963
<212> DNA
<213> New coronavirus ()
<400> 77
taggaattgt caacaacaca gtttatgatc ctttgcaacc tgaattagac tcattcaagg 60
aggagttaga taaatatttt aagaatcata catcaccaga tgttgattta ggtgacatct 120
ctggcattaa tgcttcagtt gtaaacattc aaaaagaaat tgaccgcctc aatgaggttg 180
ccaagaattt aaatgaatct ctcatcgatc tccaagaact tggaaagtat gagcagtata 240
taaaatggcc atggtacatt tggctaggtt ttatagctgg cttgattgcc atagtaatgg 300
tgacaattat gctttgctgt atgaccagtt gctgtagttg tctcaagggc tgttgttctt 360
gtggatcctg ctgcaaattt gatgaagacg actctgagcc agtgctcaaa ggagtcaaat 420
tacattacac ataaacgaac ttatggattt gtttatgaga atcttcacaa ttggaactgt 480
aactttgaag caaggtgaaa tcaaggatgc tactccttca gattttgttc gcgctactgc 540
aacgataccg atacaagcct cactcccttt cggatggctt attgttggcg ttgcacttct 600
tgctgttttt cagagcgctt ccaaaatcat aaccctcaaa aagagatggc aactagcact 660
ctccaagggt gttcactttg tttgcaactt gctgttgttg tttgtaacag tttactcaca 720
ccttttgctc gttgctgctg gccttgaagc cccttttctc tatctttatg ctttagtcta 780
cttcttgcag agtataaact ttgtaagaat aataatgagg ctttggcttt gctggaaatg 840
ccgttccaaa aacccattac tttatgatgc caactatttt ctttgctggc atactaattg 900
ttacgactat tgtatacctt acaatagtgt aacttcttca attgtcatta cttcaggtga 960
tggcacaaca agtcctattt ctgaacatga ctaccagatt ggtggttata ctgaaaaatg 1020
ggaatctgga gtaaaagact gtgttgtatt acacagttac ttcacttcag actattacca 1080
gctgtactca actcaattga gtacagacac tggtgttgaa catgttacct tcttcatcta 1140
caataaaatt gttgatgagc ctgaagaaca tgtccaaatt cacacaatcg acggttcatc 1200
cggagttgtt aatccagtaa tggaaccaat ttatgatgaa ccgacgacga ctactagcgt 1260
gcctttgtaa gcacaagctg atgagtacga acttatgtac tcattcgttt tggaagagac 1320
aggtacgtta atagttaata gcgtacttct ttttcttgct ttcgtggtat tcttgctagt 1380
tacactagcc atccttactg cgcttcgatt gtgtgcgtac tgctgcaata ttgttaacgt 1440
gagtcttgta aaaccttctt tttacgttta ctctcgtgtt aaaaatctga attcttctag 1500
agttcctgat cttctggtct aaacgaacta aatattatat tagtttttct gtttggaact 1560
ttaattttag ccatggcaga ttccaacggt actattaccg ttgaagagct taaaaagctc 1620
cttgaacaat ggaacctagt aataggtttc ctattcctta catggatttg tcttctacaa 1680
tttgcctatg ccaacaggaa taggtttttg tatataatta agttaatttt cctctggctg 1740
ttatggccag taactttagc ttgttttgtg cttgctgctg tttacagaat aaattggatc 1800
accggtggaa ttgctatcgc aatggcttgt cttgtaggct tgatgtggct cagctacttc 1860
attgcttctt tcagactgtt tgcgcgtacg cgttccatgt ggtcattcaa tccagaaact 1920
aacattcttc tcaacgtgcc actccatggc actattctga ccagaccgct tctagaaagt 1980
gaactcgtaa tcggagctgt gatccttcgt ggacatcttc gtattgctgg acaccatcta 2040
ggacgctgtg acatcaagga cctgcctaaa gaaatcactg ttgctacatc acgaacgctt 2100
tcttattaca aattgggagc ttcgcagcgt gtagcaggtg actcaggttt tgctgcatac 2160
agtcgctaca ggattggcaa ctataaatta aacacagacc attccagtag cagtgacaat 2220
attgctttgc ttgtacagta agtgacaaca gatgtttcat ctcgttgact ttcaggttac 2280
tatagcagag atattactaa ttattatgag gacttttaaa gtttccattt ggaatcttga 2340
ttacatcata aacctcataa ttaaaaattt atctaagtca ctaactgaga ataaatattc 2400
tcaattagat gaagagcaac caatggagat tgattaaacg aacatgaaaa ttattctttt 2460
cttggcactg ataacactcg ctacttgtga gctttatcac taccaagagt gtgttagagg 2520
tacaacagta cttttaaaag aaccttgctc ttctggaaca tacgagggca attcaccatt 2580
tcatcctcta gctgataaca aatttgcact gacttgcttt agcactcaat ttgcttttgc 2640
ttgtcctgac ggcgtaaaac acgtctatca gttacgtgcc agatcagttt cacctaaact 2700
gttcatcaga caagaggaag ttcaagaact ttactctcca atttttctta ttgttgcggc 2760
aatagtgttt ataacacttt gcttcacact caaaagaaag acagaatgat tgaactttca 2820
ttaattgact tctatttgtg ctttttagcc tttctgctat tccttgtttt aattatgctt 2880
attatctttt ggttctcact tgaactgcaa gatcataatg aaacttgtca cgcctaaacg 2940
aacatgaaat ttcttgtttt cttaggaatc atcacaactg tagctgcatt tcaccaagaa 3000
tgtagtttac agtcatgtac tcaacatcaa ccatatgtag ttgatgaccc gtgtcctatt 3060
cacttctatt ctaaatggta tattagagta ggagctagaa aatcagcacc tttaattgaa 3120
ttgtgcgtgg atgaggctgg ttctaaatca cccattcagt acatcgatat cggtaattat 3180
acagtttcct gtttaccttt tacaattaat tgccaggaac ctaaattggg tagtcttgta 3240
gtgcgttgtt cgttctatga agacttttta gagtatcatg acgttcgtgt tgttttagat 3300
ttcatctaaa cgaacaaact aaaatgtctg ataatggacc ccaaaatcag cgaaatgcac 3360
cccgcattac gtttggtgga ccctcagatt caactggcag taaccagaat ggagaacgca 3420
gtggggcgcg atcaaaacaa cgtcggcccc aaggtttacc caataatact gcgtcttggt 3480
tcaccgctct cactcaacat ggcaaggaag accttaaatt ccctcgagga caaggcgttc 3540
caattaacac caatagcagt ccagatgacc aaattggcta ctaccgaaga gctaccagac 3600
gaattcgtgg tggtgacggt aaaatgaaag atctcagtcc aagatggtat ttctactacc 3660
taggaactgg gccagaagct ggacttccct atggtgctaa caaagacggc atcatatggg 3720
ttgcaactga gggagccttg aatacaccaa aagatcacat tggcacccgc aatcctgcta 3780
acaatgctgc aatcgtgcta caacttcctc aaggaacaac attgccaaaa ggcttctacg 3840
cagaagggag cagaggcggc agtcaagcct cttctcgttc ctcatcacgt agtcgcaaca 3900
gttcaagaaa ttcaactcca ggcagcagta ggggaacttc tcctgctaga atggctggca 3960
atggcggtga tgctgctctt gctttgctgc tgcttgacag attgaaccag cttgagagca 4020
aaatgtctgg taaaggccaa caacaacaag gccaaactgt cactaagaaa tctgctgctg 4080
aggcttctaa gaagcctcgg caaaaacgta ctgccactaa agcatacaat gtaacacaag 4140
ctttcggcag acgtggtcca gaacaaaccc aaggaaattt tggggaccag gaactaatca 4200
gacaaggaac tgattacaaa cattggccgc aaattgcaca atttgccccc agcgcttcag 4260
cgttcttcgg aatgtcgcgc attggcatgg aagtcacacc ttcgggaacg tggttgacct 4320
acacaggtgc catcaaattg gatgacaaag atccaaattt caaagatcaa gtcattttgc 4380
tgaataagca tattgacgca tacaaaacat tcccaccaac agagcctaaa aaggacaaaa 4440
agaagaaggc tgatgaaact caagccttac cgcagagaca gaagaaacag caaactgtga 4500
ctcttcttcc tgctgcagat ttggatgatt tctccaaaca attgcaacaa tccatgagca 4560
gtgctgactc aactcaggcc taaactcatg cagaccacac aaggcagatg ggctatataa 4620
acattttcgc ttttccgttt acgatatata gtctactctt gtgcagaatg aattctcgta 4680
actacatagc acaagtagat gtagttaact ttaatctcac atagcaatct ttaatcagtg 4740
tgtaacatta gggaggactt gaaagagcca ccacattttc accgaggcca cgcggagtac 4800
gatcgagtgt acagtgaaca atgctaggga gagctgccta tatggaagag ccctaatgtg 4860
taaaattaat tttagtagtg ctatccccat gtgattttaa tagcttctta ggagaatgac 4920
aaaaaaaaaa aaaaaaaaaa aaaagcggcc gcctaacgga ttc 4963
<210> 78
<211> 61
<212> DNA
<213> Artificial sequence ()
<400> 78
tctcatgttt gacagcttat catcgatagg cgcgccatta aaggtttata ccttcccagg 60
t 61
<210> 79
<211> 53
<212> DNA
<213> Artificial sequence ()
<400> 79
ggaggttaat gttgtctact gttgtaaaca ccttaatagt cctcacttct ctc 53
<210> 80
<211> 55
<212> DNA
<213> Artificial sequence ()
<400> 80
agaagtgagg actattaagg tgtttacaac agtagacaac attaacctcc acacg 55
<210> 81
<211> 52
<212> DNA
<213> Artificial sequence ()
<400> 81
cttggagtgg tgaatccgtt aggcggccgc gcctttgcga gatgacaaca ag 52
<210> 82
<211> 70
<212> DNA
<213> Artificial sequence ()
<400> 82
ggatacaact agctacagag aagctgcttg ttgtcatctc gcaaaggcgc tctcaatgac 60
ttcagtaact 70
<210> 83
<211> 53
<212> DNA
<213> Artificial sequence ()
<400> 83
actcattgaa tcataataaa gtctagcctt accccattta ttaaatggaa aac 53
<210> 84
<211> 53
<212> DNA
<213> Artificial sequence ()
<400> 84
ttccatttaa taaatggggt aaggctagac tttattatga ttcaatgagt tat 53
<210> 85
<211> 75
<212> DNA
<213> Artificial sequence ()
<400> 85
tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttaggc ggccgcgtgg 60
tgcacaaatc gtttc 75
<210> 86
<211> 70
<212> DNA
<213> Artificial sequence ()
<400> 86
ctatgactga catagccaag aaaccaactg aaacgatttg tgcaccacgc tcactgtctt 60
ttttgatggt 70
<210> 87
<211> 54
<212> DNA
<213> Artificial sequence ()
<400> 87
gtctaattca ggttgcaaag gatcataaac tgtgttgttg acaattccta ttac 54
<210> 88
<211> 54
<212> DNA
<213> Artificial sequence ()
<400> 88
taggaattgt caacaacaca gtttatgatc ctttgcaacc tgaattagac tcat 54
<210> 89
<211> 57
<212> DNA
<213> Artificial sequence ()
<400> 89
gaatccgtta ggcggccgct tttttttttt tttttttttt tttgtcattc tcctaag 57
<210> 90
<211> 3143
<212> DNA
<213> Artificial sequence ()
<400> 90
tgttgtcatc tcgcaaaggc gcggccgcct aacggattca ccactccaag aattggagcc 60
aatcaattct tgcggagaac tgtgaatgcg caaaccaacc cttggcagaa catatccatc 120
gcgtccgcca tctccagcag ccgcacgcgg cgcatctcgg gcagcgttgg gtcctggcca 180
cgggtgcgca tgatcgtgct cctgtcgttg aggacccggc taggctggcg gggttgcctt 240
actggttagc agaatgaatc accgatacgc gagcgaacgt gaagcgactg ctgctgcaaa 300
acgtctgcga cctgagcaac aacatgaatg gtcttcggtt tccgtgtttc gtaaagtctg 360
gaaacgcgga agtcagcgcc ctgcaccatt atgttccgga tctgcatcgc aggatgctgc 420
tggctaccct gtggaacacc tacatctgta ttaacgaagc gctggcattg accctgagtg 480
atttttctct ggtcccgccg catccatacc gccagttgtt taccctcaca acgttccagt 540
aaccgggcat gttcatcatc agtaacccgt atcgtgagca tcctctctcg tttcatcggt 600
atcattaccc ccatgaacag aaatccccct tacacggagg catcagtgac caaacaggaa 660
aaaaccgccc ttaacatggc ccgctttatc agaagccaga cattaacgct tctggagaaa 720
ctcaacgagc tggacgcgga tgaacaggca gacatctgtg aatcgcttca cgaccacgct 780
gatgagcttt accgcagctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac 840
atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc 900
cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt 960
agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag 1020
tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc 1080
gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg 1140
tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa 1200
agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 1260
cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 1320
ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 1380
tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 1440
gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc 1500
gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 1560
gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 1620
ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 1680
ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag 1740
ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 1800
gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc 1860
ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt 1920
tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt 1980
ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca 2040
gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg 2100
tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac 2160
cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg 2220
ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc 2280
gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgctg 2340
caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac 2400
gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc 2460
ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac 2520
tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact 2580
caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa 2640
cacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt 2700
cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca 2760
ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa 2820
aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac 2880
tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg 2940
gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc 3000
gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata 3060
ggcgtatcac gaggcccttt cgtcttcaag aattctcatg tttgacagct tatcatcgat 3120
aggcgcgcca ttaaaggttt ata 3143
<210> 91
<211> 53
<212> DNA
<213> Artificial sequence ()
<400> 91
tgttgtcatc tcgcaaaggc gcggccgcct aacggattca ccactccaag aat 53
<210> 92
<211> 57
<212> DNA
<213> Artificial sequence (primer)
<400> 92
tataaacctt taatggcgcg cctatcgatg ataagctgtc aaacatgaga attcttg 57
<210> 93
<211> 6498
<212> DNA
<213> Artificial sequence ()
<400> 93
aatagcttct taggagaatg acaaaaaaaa aaaaaaaaaa gcggccgctt ttttccttgt 60
cgacagcgac acacttgcat cggatgcagc ccggttaacg tgccggcacg gcctgggtaa 120
ccaggtattt tgtccacata accgtgcgca aaatgttgtg gataagcagg acacagcagc 180
aatccacagc aggcatacaa ccgcacaccg aggttactcc gttctacagg ttacgacgac 240
atgtcaatac ttgcccttga caggcattga tggaatcgta gtctcacgct gatagtctga 300
tcgacaatac aagtgggacc gtggtcccag accgataatc agaccgacaa cacgagtggg 360
atcgtggtcc cagactaata atcagaccga cgatacgagt gggaccgtgg tcccagacta 420
ataatcagac cgacgatacg agtgggaccg tggttccaga ctaataatca gaccgacgat 480
acgagtggga ccgtggtccc agactaataa tcagaccgac gatacgagtg ggaccatggt 540
cccagactaa taatcagacc gacgatacga gtgggaccgt ggtcccagtc tgattatcag 600
accgacgata cgagtgggac cgtggtccca gactaataat cagaccgacg atacgagtgg 660
gaccgtggtc ccagactaat aatcagaccg acgatacgag tgggaccgtg gtcccagtct 720
gattatcaga ccgacgatac aagtggaaca gtgggcccag agagaatatt caggccagtt 780
atgctttctg gcctgtaaca aaggacatta agtaaagaca gataaacgta gactaaaacg 840
tggtcgcatc agggtgctgg cttttcaagt tccttaagaa tggcctcaat tttctctata 900
cactcagttg gaacacgaga cctgtccagg ttaagcacca ttttatcgcc cttatacaat 960
actgtcgctc caggagcaaa ctgatgtcgt gagcttaaac tagttcttga tgcagatgac 1020
gttttaagca cagaagttaa aagagtgata acttcttcag cttcaaatat caccccagct 1080
tttttctgct catgaaggtt agatgcctgc tgcttaagta attcctcttt atctgtaaag 1140
gctttttgaa gtgcatcacc tgaccgggca gatagttcac cggggtgaga aaaaagagca 1200
acaactgatt taggcaattt ggcggtgttg atacagcggg taataatctt acgtgaaata 1260
ttttccgcat cagccagcgc agaaatattt ccagcaaatt cattctgcaa tcggcttgca 1320
taacgctgac cacgttcata agcacttgtt gggcgataat cgttacccaa tctggataat 1380
gcagccatct gctcatcatc cagctcgcca accagaacac gataatcact ttcggtaagt 1440
gcagcagctt tacgacggcg actcccatcg gcaatttcta tgacaccaga tactcttcga 1500
ccgaacgccg gtgtctgttg accagtcagt agaaaagaag ggatgagatc atccagtgcg 1560
tcctcagtaa gcagctcctg gtcacgttca ttacctgacc atacccgaga ggtcttctca 1620
acactatcac cccggagcac ttcaagagta aacttcacat cccgaccaca tacaggcaaa 1680
gtaatggcat taccgcgagc cattactcct acgcgcgcaa ttaacgaatc caccatcggg 1740
gcagctggtg tcgataacga agtatcttca accggttgag tattgagcgt atgttttgga 1800
ataacaggcg cacgcttcat tatctaatct cccagcgtgg tttaatcaga cgatcgaaaa 1860
tttcattgca gacaggttcc caaatagaaa gagcatttct ccaggcacca gttgaagagc 1920
gttgatcaat ggcctgttca aaaacagttc tcatccggat ctgaccttta ccaacttcat 1980
ccgtttcacg tacaacattt tttagaacca tgcttcccca ggcatcccga atttgctcct 2040
ccatccacgg ggactgagag ccattactat tgctgtattt ggtaagcaaa atacgtacat 2100
caggctcgaa ccctttaaga tcaacgttct tgagcagatc acgaagcata tcgaaaaact 2160
gcagtgcgga ggtgtagtca aacaactcag caggcgtggg aacaatcagc acatcagcag 2220
cacatacgac attaatcgtg ccgataccca ggttaggcgc gctgtcaata actatgacat 2280
catagtcatg agcaacagtt tcaatggcca gtcggagcat caggtgtgga tcggtgggca 2340
gtttaccttc atcaaatttg cccattaact cagtttcaat acggtgcaga gccagacagg 2400
aaggaataat gtcaagcccc ggccagcaag tgggctttat tgcataagtg acatcgtcct 2460
tttccccaag atagaaaggc aggagagtgt cttctgcatg aatatgaaga tctggtaccc 2520
atccgtgata cattgaggct gttccctggg ggtcgttacc ttccacgagc aaaacacgta 2580
gccccttcag agccagatcc tgagcaagat gaacagaaac tgaggttttg taaacgccac 2640
ctttatgggc agcaaccccg atcaccggtg gaaatacgtc ttcagcacgt cgcaatcgcg 2700
taccaaacac atcacgcata tgattaattt gttcaattgt ataaccaaca cgttgctcaa 2760
cccgtcctcg aatttccata tccgggtgcg gtagtcgccc tgctttctcg gcatctctga 2820
tagcctgaga agaaacccca actaaatccg ctgcttcacc tattctccag cgccgggtta 2880
ttttcctcgc ttccgggctg tcatcattaa actgtgcaat ggcgatagcc ttcgtcattt 2940
catgaccagc gtttatgcac tggttaagtg tttccatgag tttcattctg aacatccttt 3000
aatcattgct ttgcgttttt ttattaaatc ttgcaattta ctgcaaagca acaacaaaat 3060
cgcaaagtca tcaaaaaacc gcaaagttgt ttaaaataag agcaacacta caaaaggaga 3120
taagaagagc acatacctca gtcacttatt atcactagcg ctcgccgcag ccgtgtaacc 3180
gagcatagcg agcgaactgg cgaggaagca aagaagaact gttctgtcag atagctctta 3240
cgctcagcgc aagaagaaat atccaccgtg ggaaaaactc caggtagagg tacacacgcg 3300
gatagccaat tcagagtaat aaactgtgat aatcaaccct catcaatgat gacgaactaa 3360
cccccgatat caggtcacat gacgaaggga aagagaagga aatcaactgt gacaaactgc 3420
cctcaaattt ggcttcctta aaaattacag ttcaaaaagt atgagaaaat ccatgcaggc 3480
tgaaggaaac agcaaaactg tgacaaatta ccctcagtag gtcagaacaa atgtgacgaa 3540
ccaccctcaa atctgtgaca gataaccctc agactatcct gtcgtcatgg aagtgatatc 3600
gcggaaggaa aatacgatat gagtcgtctg gcggcctttc tttttctcaa tgtatgagag 3660
gcgcattgga gttctgctgt tgatctcatt aacacagacc tgcaggaagc ggcggcggaa 3720
gtcaggcata cgctggtaac tttgaggcag ctggtaacgc tctatgatcc agtcgatttt 3780
cagagagacg atgcctgagc catccggctt acgatactga cacagggatt cgtataaacg 3840
catggcatac ggattggtga tttcttttgt ttcactaagc cgaaactgcg taaaccggtt 3900
ctgtaacccg ataaagaagg gaatgagata tgggttgata tgtacactgt aaagccctct 3960
ggatggactg tgcgcacgtt tgataaacca aggaaaagat tcatagcctt tttcatcgcc 4020
ggcatcctct tcagggcgat aaaaaaccac ttccttcccc gcgaaactct tcaatgcctg 4080
ccgtatatcc ttactggctt ccgcagaggt caatccgaat atttcagcat atttagcaac 4140
atggatctcg cagataccgt catgttcctg tagggtgcca tcagattttc tgatctggtc 4200
aacgaacaga tacagcatac gtttttgatc ccgggagaga ctatatgccg cctcagtgag 4260
gtcgtttgac tggacgattc gcgggctatt tttacgtttc ttgtgattga taaccgctgt 4320
ttccgccatg acagatccat gtgaagtgtg acaagttttt agattgtcac actaaataaa 4380
aaagagtcaa taagcaggga taactttgtg aaaaaacagc ttcttctgag ggcaatttgt 4440
cacagggtta agggcaattt gtcacagaca ggactgtcat ttgagggtga tttgtcacac 4500
tgaaagggca atttgtcaca acaccttctc tagaaccagc atggataaag gcctacaagg 4560
cgctctaaaa aagaagatct aaaaactata aaaaaaataa ttataaaaat atccccgtgg 4620
ataagtggat aaccccaagg gaagtttttt caggcatcgt gtgtaagcag aatatataag 4680
tgctgttccc tggtgcttcc tcgctcactc gagggcttcg ccctgtcgct cgactgcggc 4740
gagcactact ggctgtaaaa ggacagacca catcatggtt ctgtgttcat taggttgttc 4800
tgtccattgc tgacataatc cgctccactt caacgtaaca ccgcacgaag atttctattg 4860
ttcctgaagg catattcaaa tcgttttcgt taccgcttgc aggcatcatg acagaacact 4920
acttcctata aacgctacac aggctcctga gattaataat gcggatctct acgataatgg 4980
gagattttcc cgactgtttc gttcgcttct cagtggataa cagccagctt ctctgtttaa 5040
cagacaaaaa cagcatatcc actcagttcc acatttccat ataaaggcca aggcatttat 5100
tctcaggata attgtttcag catcgcaacc gcatcagact ccggcatcgc aaactgcacc 5160
cggtgccggg cagccacatc cagcgcaaaa accttcgtgt agacttccgt tgaactgatg 5220
gacttatgtc ccatcaggct ttgcagaact ttcagcggta taccggcata cagcatgtgc 5280
atcgcatagg aatggcggaa cgtatgtggt gtgaccggaa cagagaacgt cacaccgtca 5340
gcagcagcgg cggcaaccgc ctccccaatc caggtcctga ccgttctgtc cgtcacttcc 5400
cagatccgcg ctttctctgt ccttcctgtg cgacggttac gccgctccat gagcttatcg 5460
cgaataaata cctgtgacgg aagatcactt cgcagaataa ataaatcctg gtgtccctgt 5520
tgataccggg aagccctggg ccaacttttg gcgaaaatga gacgttgatc ggcacgtaag 5580
aggttccaac tttcaccata atgaaataag atcactaccg ggcgtatttt ttgagttatc 5640
gagattttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat ataccaccgt 5700
tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg 5760
tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaaaa 5820
taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc 5880
ggaattccgt atggcaatga aagacggtga gctggtgata tgggatagtg ttcacccttg 5940
ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg aataccacga 6000
cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg gtgaaaacct 6060
ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtctcagcca atccctgggt 6120
gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg cccccgtttt 6180
caccatgggc aaatattata cgcaaggcga caaggtgctg atgccgctgg cgattcaggt 6240
tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg cttaatgaat tacaacagta 6300
ctgcgatgag tggcagggcg gggcgtaatt tttttaaggc agttattggt gcccttaaac 6360
gcctggttgc tacgcctgaa taagtgataa taagcggatg aatggcagaa attcgatgat 6420
aagctgtcaa acatgagaat tggtcgactt ggcgcgccat taaaggttta taccttccca 6480
ggtaacaaac caaccaac 6498
<210> 94
<211> 72
<212> DNA
<213> Artificial sequence ()
<400> 94
aatagcttct taggagaatg acaaaaaaaa aaaaaaaaaa gcggccgctt ttttccttgt 60
cgacagcgac ac 72
<210> 95
<211> 68
<212> DNA
<213> Artificial sequence ()
<400> 95
gttggttggt ttgttacctg ggaaggtata aacctttaat ggcgcgccaa gtcgaccaat 60
tctcatgt 68
<210> 96
<211> 70
<212> DNA
<213> Artificial sequence ()
<400> 96
agcacatatt cagtggatgg ttatgttcac acctttagta cctttctgga cattcaaata 60
tgtatccgct 70
<210> 97
<211> 70
<212> DNA
<213> Artificial sequence ()
<400> 97
ttcataatta gggttaagca tgtcttcaga ggtgcagatc acatgtcttg aactagaagg 60
cacagtcgag 70
<210> 98
<211> 586
<212> DNA
<213> New coronavirus ()
<400> 98
agcacatatt cagtggatgg ttatgttcac acctttagta cctttctgga taacaattgc 60
ttatatcatt tgtatttcca caaagcattt ctattggttc tttagtaatt acctaaagag 120
acgtgtagtc tttaatggtg tttcctttag tacttttgaa gaagctgcgc tgtgcacctt 180
tttgttaaat aaagaaatgt atctaaagtt gcgtagtgat gtgctattac ctcttacgca 240
atataataga tacttagctc tttataataa gtacaagtat tttagtggag caatggatac 300
aactagctac agagaagctg cttgttgtca tctcgcaaag gctctcaatg acttcagtaa 360
ctcaggttct gatgttcttt accaaccacc acaaacctct atcacctcag ctgttttgca 420
gagtggtttt agaaaaatgg cattcccatc tggtaaagtt gagggttgta tggtacaagt 480
aacttgtggt acaactacac ttaacggtct ttggcttgat gacgtagttt actgtccaag 540
acatgtgatc tgcacctctg aagacatgct taaccctaat tatgaa 586
<210> 99
<211> 25
<212> DNA
<213> Artificial sequence ()
<400> 99
agcacatatt cagtggatgg ttatg 25
<210> 100
<211> 21
<212> DNA
<213> Artificial sequence ()
<400> 100
ttcataatta gggttaagca t 21
<210> 101
<211> 70
<212> DNA
<213> Artificial sequence ()
<400> 101
cactttgatg gacaacaggg tgaagtacca gtttctatca ttaataacac cattcaaata 60
tgtatccgct 70
<210> 102
<211> 70
<212> DNA
<213> Artificial sequence ()
<400> 102
tataccgttc aatgaattca tccatagcta attctaagaa atcaatttcc aactagaagg 60
cacagtcgag 70
<210> 103
<211> 637
<212> DNA
<213> New coronavirus ()
<400> 103
cactttgatg gacaacaggg tgaagtacca gtttctatca ttaataacac tgtttacaca 60
aaagttgatg gtgttgatgt agaattgttt gaaaataaaa caacattacc tgttaatgta 120
gcatttgagc tttgggctaa gcgcaacatt aaaccagtac cagaggtgaa aatactcaat 180
aatttgggtg tggacattgc tgctaatact gtgatctggg actacaaaag agatgctcca 240
gcacatatat ctactattgg tgtttgttct atgactgaca tagccaagaa accaactgaa 300
acgatttgtg caccactcac tgtctttttt gatggtagag ttgatggtca agtagactta 360
tttagaaatg cccgtaatgg tgttcttatt acagaaggta gtgttaaagg tttacaacca 420
tctgtaggtc ccaaacaagc tagtcttaat ggagtcacat taattggaga agccgtaaaa 480
acacagttca attattataa gaaagttgat ggtgttgtcc aacaattacc tgaaacttac 540
tttactcaga gtagaaattt acaagaattt aaacccagga gtcaaatgga aattgatttc 600
ttagaattag ctatggatga attcattgaa cggtata 637
<210> 104
<211> 21
<212> DNA
<213> Artificial sequence ()
<400> 104
cactttgatg gacaacaggg t 21
<210> 105
<211> 24
<212> DNA
<213> Artificial sequence ()
<400> 105
tataccgttc aatgaattca tcca 24
<210> 106
<211> 70
<212> DNA
<213> Artificial sequence ()
<400> 106
ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca cattcaaata 60
tgtatccgct 70
<210> 107
<211> 70
<212> DNA
<213> Artificial sequence ()
<400> 107
caacgacagt aattagttat taattatact gcgtgagtgc actaagcatg aactagaagg 60
cacagtcgag 70
<210> 108
<211> 70
<212> DNA
<213> Artificial sequence ()
<400> 108
cgcggagtac gatcgagtgt acagtgaaca atgctaggga gagctgccta cattcaaata 60
tgtatccgct 70
<210> 109
<211> 70
<212> DNA
<213> Artificial sequence ()
<400> 109
gatcagacta tcagcgtgag actacgattc catcaatgcc tgtcaagggc aactagaagg 60
cacagtcgag 70
<210> 110
<211> 24
<212> DNA
<213> Artificial sequence ()
<400> 110
ggcaaatatt atacgcaagg cgac 24
<210> 111
<211> 39
<212> DNA
<213> Artificial sequence ()
<400> 111
caacgacagt aattagttat taattatact gcgtgagtg 39
<210> 112
<211> 22
<212> DNA
<213> Artificial sequence ()
<400> 112
cgcggagtac gatcgagtgt ac 22
<210> 113
<211> 23
<212> DNA
<213> Artificial sequence ()
<400> 113
gatcagacta tcagcgtgag act 23
<210> 114
<211> 445
<212> DNA
<213> Artificial sequence ()
<400> 114
ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat 60
gccgtttgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat 120
gagtggcagg gcggggcgta atttttttaa ggcagttatt ggtgccctta aacgcctggt 180
tgctacgcct gaataagtga taataagcgg atgaatggca gaaattcgat gataagctgt 240
caaacatgag aattggtcga cttggcgcgc ctaatacgac tcactatagg attaaaggtt 300
tataccttcc caggtaacaa accaaccaac tttcgatctc ttgtagatct gttctctaaa 360
cgaactttaa aatctgtgtg gctgtcactc ggctgcatgc ttagtgcact cacgcagtat 420
aattaataac taattactgt cgttg 445
<210> 115
<211> 470
<212> DNA
<213> Artificial sequence ()
<400> 115
cgcggagtac gatcgagtgt acagtgaaca atgctaggga gagctgccta tatggaagag 60
ccctaatgtg taaaattaat tttagtagtg ctatccccat gtgattttaa tagcttctta 120
ggagaatgac aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ctagcataac cccttggggc 180
ctctaaacgg gtcttgaggg gttttttggc ggccgctttt ttccttgtcg acagcgacac 240
acttgcatcg gatgcagccc ggttaacgtg ccggcacggc ctgggtaacc aggtattttg 300
tccacataac cgtgcgcaaa atgttgtgga taagcaggac acagcagcaa tccacagcag 360
gcatacaacc gcacaccgag gttactccgt tctacaggtt acgacgacat gtcaatactt 420
gcccttgaca ggcattgatg gaatcgtagt ctcacgctga tagtctgatc 470
Claims (5)
1. A full-length cDNA clone single-copy plasmid pBacm-T7p-SARS-CoV-2-FL-T7T of SARS-CoV-2 vaccine strain, the size of said plasmid is about 36.4kb, and its nucleotide sequence is SEQ ID NO. 1. Wherein, the sequence of the pBacm vector is shown as SEQ ID NO.3, and the sequence of SARS-CoV-2 is shown as SEQ ID NO. 2.
2. The method of claim 1 wherein the plasmid pBacm-T7p-SARS-CoV-2-FL-T7T is constructed by the steps of:
(1) constructing a pBacm vector and modifying enzyme cutting sites added on two sides of the pBacm vector;
(2) constructing tool plasmids pBacm-SacB-Kan and pBacm-SacB-Amp containing forward and reverse screening markers;
(3) constructing a full-length cDNA single-copy plasmid of the SARS-CoV-2 vaccine strain;
(4) connecting the SARS-CoV-2 full-length genome sequence with the single copy carrier pBacm to obtain pBacm-SARS-CoV-2- (FL &2GC) plasmid;
(5) repairing redundant GC bases of a site in the pBacm-SARS-CoV-2- (FL &2GC) recombinant plasmid, and simultaneously cloning a T7 promoter sequence and a T7 terminator sequence into the single-copy vector to obtain a target vector pBacm-T7 p-SARS-CoV-2-FL-T7T.
3. The method according to claim 2, wherein the specific steps of step (1) comprise:
1) taking pBacm plasmid as a template, reversely amplifying the large plasmid ring through PCR, and respectively introducing NotI and AscI enzyme cutting sites at the 5 'end and the 3' end of an amplification product;
2) using pY44 plasmid as a template, amplifying a kala antibiotic gene (Kan) by PCR, and respectively introducing restriction enzyme AscI and NotI enzyme cutting sites at the 5 'end and the 3' end of an amplification product;
3) the PCR products in 1) and 2) were purified, digested with AscI and NotI, and ligated with T4 DNA ligase to obtain pBacm-AscI-Kan-NotI plasmid.
4. The method according to claim 2, wherein the specific step of step (2) comprises:
1) designing a primer to amplify a sucrose lethal gene SacB by taking a pK18mob-SacB plasmid as a template, wherein the 5 'end and the 3' end of the amplification primer respectively contain AscI enzyme cutting sites and NotI enzyme cutting sites;
2) purifying the SacB gene PCR product, and then carrying out enzyme digestion by AscI and NotI;
3) digesting the constructed pBacm-AscI-Kan-NotI plasmid by AscI and NotI;
4) purifying the enzyme digestion products in 2) and 3), and then connecting with T4 DNA ligase to obtain a recombinant plasmid pBacm-SacB;
5) respectively taking pY44 and pBR322 plasmids as templates, designing primers to amplify Kan and ampicillin (Amp) genes, wherein the 5 'end and the 3' end of the amplification primers respectively contain 50bp sequences which are homologous with the two sides of the end of the SacB gene in pBacm-SacB;
6) after the PCR product of the resistance gene in 5) is purified, the Kan or Amp gene is respectively inserted into a pBacm-SacB vector to obtain recombinant plasmids pBacm-SacB-Kan and pBacm-SacB-Amp.
5. The method according to claim 2, wherein the specific step of step (3) comprises:
1) extracting SARS-CoV-2 inactivated vaccine strain RNA and reverse transcribing into cDNA;
2) using the cDNA as a template, designing primers to carry out PCR amplification respectively to obtain 17 gene segments of the SARS-CoV-2 inactivated vaccine strain, wherein each gene segment contains a homologous sequence of 15 bp; in addition, the locus T of 8791 and the locus T of 12655 are respectively synonymously mutated into C, and two BglI endonuclease sequences are introduced to be used as genetic markers so as to be distinguished from wild viruses;
3) the gene fragments in 2) are respectively connected to pUC19 vector which is cut by KpnI and HindIII by taking the length of every 5kb as a unit, so as to obtain 6 recombinant plasmids;
4) using the 6 recombinant plasmids as templates, designing primers to perform PCR amplification respectively to obtain 6 SARS-CoV-2 genome fragments with each length of about 5 kb;
5) the 6-segment gene fragment in 4) is gradually connected to the linearized pBR322 vector by 3 times, and 10kb of each connection is carried out to obtain recombinant plasmids containing SARS-CoV-210 kb, 20kb and full-length 30kb respectively.
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