CN113913313A - Single-spore saccharomyces cerevisiae strain and application thereof - Google Patents
Single-spore saccharomyces cerevisiae strain and application thereof Download PDFInfo
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- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/14—Yeasts or derivatives thereof
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
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- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9728—Fungi, e.g. yeasts
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Abstract
The invention provides a single-spore saccharomyces cerevisiae strain and application thereof. The saccharomyces cerevisiae strain provided by the invention is a saccharomyces cerevisiae Xuelian3208-3 strain (Kazachstania uniispora Xuelian3208-3), which is preserved in China Center for Type Culture Collection (CCTCC) at 7-2 months in 2021, and the preservation number is CCTCC NO: M2021812. The saccharomyces cerevisiae monospora strain provided by the invention can efficiently remove DPPH and OH free radicals and has stronger tyrosinase inhibitory activity.
Description
Technical Field
The invention belongs to the field of microorganisms, and particularly relates to a single-spore saccharomyces cerevisiae strain and application thereof.
Background
The Tibetan saussurea involucrate, also named Tibetan spiritual mushroom, is a special rare strain from Tibetan snowfield, is a leaven of traditional yoghourt in Tibetan Linzhi, is shaped like white colloidal particles, has complex and diverse bacterial phase structures, and is rich in various strain resources.
With the upgrading of domestic resident consumption level, the development of cosmetics industry has entered a new mileage. At present, the natural skin care product is mainly prepared from plant extracts, and the plant extracts are applied to cosmetics as active ingredients, and generally have the characteristics of moisturizing, skin care, sun protection, sterilization, melanin generation inhibition, free radical removal, acne removal, corrosion prevention, low toxicity, environmental friendliness and the like. With the increasing requirements of people on skin care products, natural skin care products taking plant extracts as the main components are gradually unable to meet the consumption requirements of people. The natural components contained in the plant extract are easier to be absorbed by skin after being treated by certain directional technical means, such as biotechnology, namely microbial fermentation, enzymolysis and the like, so that the effect of the product is more obvious and the functionality is richer.
Disclosure of Invention
The invention aims to provide a saccharomyces cerevisiae Xuelian3208-3 strain (Kazachstania unispora Xuelian3208-3) with efficient free radical scavenging capacity and tyrosinase inhibitory activity.
The saccharomyces cerevisiae Xueliana 3208-3 strain (Kazachstania unispora Xueliana 3208-3) provided by the invention is separated from Tibetan mushroom for self-making yoghourt by herdsmen in Tibetan glossy ganoderma areas, is preserved in China Center for Type Culture Collection (CCTCC) at 7 months and 2 days in 2021, and has a preservation number of CCTCC NO: M2021812.
Preferably, the 26S rDNA gene sequence of the saccharomyces cerevisiae strain Xuelian3208-3 is shown in SEQ ID NO. 2.
The invention also provides a fermentation preparation method of the single-spore saccharomyces cerevisiae microbial inoculum, which comprises the following steps: and culturing the saccharomyces cerevisiae monospora strain.
Preferably, the preparation method comprises the following steps:
(1) amplifying and culturing the single-spore saccharomyces cerevisiae strain;
(2) adding the product obtained in the step (1) into a liquid culture medium, and fermenting and culturing at the temperature of 26-32 ℃.
The invention also provides a microbial inoculum which is obtained by the fermentation preparation method.
The invention also provides the single-spore saccharomyces cerevisiae strain or a fermentation product obtained by fermenting the microbial inoculum.
Preferably, the fermentation product has free radical scavenging ability and/or tyrosinase inhibitory activity.
Preferably, the fermentation product has a DPPH-radical clearance of greater than 95%;
alternatively, preferably, the fermentation product has a clearance of OH radicals of greater than 80%;
alternatively, preferably, the fermentation product has greater than 88% inhibition of tyrosinase activity.
The invention also provides a preparation method of the fermentation product, which comprises the step of culturing the saccharomyces cerevisiae strain or the microbial inoculum.
Preferably, the preparation method comprises the following steps:
(1) amplifying and culturing the single-spore saccharomyces cerevisiae strain or the microbial inoculum;
(2) adding the product obtained in the step (1) into a liquid culture medium, and fermenting and culturing at 26-32 ℃ to obtain the fermentation product.
The invention also provides application of the saccharomyces cerevisiae monospora strain or the microbial inoculum in antioxidant, whitening and anti-aging products.
Preferably, the product is a food, cosmetic or nutraceutical.
The saccharomyces cerevisiae Xuelian3208-3 strain (Kazachstania unispora Xuelian3208-3) provided by the invention has high-efficiency free radical scavenging capacity and tyrosinase inhibitory activity, and is obtained by separating Tibetan mushrooms for home-made yoghourt by herders in the Tibetan glossy ganoderma region. The strain provided by the invention supplements yeast resources with free radical scavenging capacity and tyrosinase inhibitory activity. The strain provided by the invention can be applied to high-efficiency antioxidant, anti-aging and whitening products. The strain provided by the invention can maximally retain natural active ingredients in cosmetic raw materials and can generate new effective active ingredients. The product filtrate obtained by fermenting the saccharomyces cerevisiae Xuelian3208-3 strain provided by the invention contains abundant minerals, amino acids, beta-glucan, organic acids, vitamins and other substances.
The fermentation liquor of the saccharomyces cerevisiae Xuelian3208-3 (Kazachstania uniispora Xuelian3208-3) provided by the invention can efficiently remove DPPH and OH free radicals, the DPPH and OH free radical removal rate reaches 95.33%, OH free radical removal rate reaches 80.28%, and the strain has strong tyrosinase inhibitory activity, and the obtained test result proves that the strain has the functions of resisting oxidation and whitening.
Information on strain preservation
The saccharomyces cerevisiae Xueliana 3208-3 strain (Kazachstania uniispora Xueliana 3208-3) provided by the invention is preserved in China Center for Type Culture Collection (CCTCC) at 7 months and 2 days of 2021, and the preservation number is CCTCC NO: m2021812, deposit address: china, wuhan university, zip code: 430072; telephone: 027-68754052.
Kluyveromyces lactis KW-6(Kluyveromyces marxiaus KW-6) in the embodiment of the invention is preserved in China Center for Type Culture Collection (CCTCC) in 6 months and 2 days in 2020, the preservation number is CCTCC NO: M2020173, the preservation address is as follows: china, wuhan university, zip code: 430072; telephone: 027-68754052.
Drawings
FIG. 1 shows "Tibet saussurea involucrata" after washing with sterile physiological saline;
FIG. 2 shows a colony morphology of Saccharomyces cerevisiae Xuelian3208-3 strain;
FIG. 3 shows the strain morphology profile of Saccharomyces cerevisiae Xuelian3208-3 strain.
Detailed Description
The saccharomyces cerevisiae Xueliana 3208-3 strain (Kazachstania uniispora Xueliana 3208-3) provided by the invention is a yeast strain which is separated and screened from 'Tibetan saussurea involucrata' collected from the Tibetan glossy ganoderma area, has high-efficiency free radical scavenging capacity and tyrosinase inhibitory activity.
The strain provided by the invention is characterized in that the strain is enriched from a Tibetan saussurea sample, a single colony is cultured, and then the strain conforming to the colony morphology of yeast is screened for performance verification. Selecting a strain with excellent free radical scavenging capacity and tyrosinase inhibition function at the same time for molecular biological identification.
Table 1 below shows the reagent and instrument source information used in the examples of the present invention.
TABLE 1 reagent and Instrument Source information Table
Example 1 enrichment, isolation and purification of Yeast from saussurea Involucrata
Enrichment: washing Tibetan herba Saussureae Involueratae with sterile normal saline, grinding with mortar, placing in 20ml sterile normal saline, and shaking for 20min as shown in figure 1. Taking 1ml of bacterial liquid to carry out gradient dilution to 1032ml of the diluted solution was added to 20ml of YPD liquid medium, followed by shaking culture at 30 ℃ and 180rpm for 24 hours.
Separation: culturing YPD medium bacteriaDiluting the solution to a concentration of 10-6-10-7And coating 100 mu L of diluted bacterial liquid on a YPD agar medium plate, and culturing at 30 ℃ for 24-48 h until bacterial colonies are formed.
And (3) purification: picking single colonies with smooth, moist and sticky surfaces, uniform colony texture, uniform color on the front and back sides, the edges and the central part, and respectively scribing on the blank YPD plates in a subarea manner. Repeating the steps for more than 3 times until the colony growing in a single YPD plate is consistent in shape and color.
And obtaining 19 yeast strains after enrichment, separation and purification.
EXAMPLE 2 determination of DPPH free radical and OH free radical scavenging efficiency of Yeast fermentation broth supernatant
The clearance of DPPH.free radical and. OH free radical was determined for the 19 yeast strains obtained in example 1 and the fermentation supernatants of the laboratory own yeast strain KW-6. The yeast fermentation broth supernatant was prepared and assayed as follows.
1. Preparation of supernatant of yeast fermentation broth
Activating the strain: 1ml of yeast liquid stored in glycerin tube was aspirated by pipette, and inoculated into 9ml of YPD liquid medium, activated and cultured at 30 ℃ and 180rpm for 24 hours.
② seed liquid culture: 1ml of the yeast activation culture was aspirated by a pipette gun, and inoculated into 9ml of YPD liquid medium and cultured at 30 ℃ and 180rpm for 16 hours.
③ fermentation: 2.5ml of yeast seed culture medium was aspirated by pipette, and inoculated into 45ml of YPD liquid medium, and shake-cultured at 30 ℃ and 180rpm for 24 hours.
Preparing supernatant of fermentation liquor: and centrifuging the fermentation liquor fermented for 24 hours at 5000rpm for 4min, and collecting the supernatant. And obtaining the supernatant of the fermentation liquor of the yeast strain.
2. Determination of DPPH & free radical clearance of yeast fermentation broth supernatant
Taking 3mL of fermentation liquor to be detected and mixing with an equal volume of 0.16mmol/L DPPH solution (A1 tube); mixing equal volume of absolute ethanol and 0.16mmol/L DPPH solution (A2 tube); taking absolute ethyl alcohol with the same volume and uniformly mixing the absolute ethyl alcohol with a solution to be detected (A3 tube); after 30 ℃ dark reaction for 30min, the absorbance values of tubes A1, A2 and A3 were measured at 519nm using distilled water as a blank and recorded as ODA1, ODA2 and ODA3, respectively. DPPH-radical clearance was calculated according to the following formula:
DPPH-clearance (OD)A2+ODA3-ODA1)/ODA2×100％。
The results of the experiment are shown in Table 2.
TABLE 2 clearance of DPPH free radical from yeast fermentation broth supernatant
3. Determination of clearance rate of yeast fermentation broth supernatant to OH free radical
Sucking 1mL of sample solution, adding 0.05mol/L of Tris-HC1-EDTA buffer solution with pH8.2 (9 mL), mixing uniformly, carrying out water bath reaction at 25 ℃ for 15min, taking 3mL of reaction solution after the reaction is finished, adding 45mmol/L of pyrogallol solution (prepared by 0.01mol L of hydrochloric acid) 0.1mL, mixing uniformly, standing for reaction for 3min, measuring the absorbance A1 of the mixture at 340nm, detecting the absorbance A2 of the mixture without the pyrogallol, replacing the sample solution with distilled water to obtain the absorbance A3, and using the distilled water as a blank. OH clearance was calculated according to the following formula:
OH clearance ═ A3- (a1-a2) ]/A3 × 100%.
The results of the experiment are shown in Table 3.
TABLE 3 clearance of OH free radical from yeast fermentation broth supernatant
From the results in tables 2 and 3, it is understood that the following 10 yeast strains have high DPPH.radical and OH.radical scavenging rates: xuelian3208-1, Xuelian3208-3, Xuelian3208-4, Xuelian3208-10, Xuelian3208-11, Xuelian3208-14, Xuelian3208-15, Xuelian3208-17, Xuelian3208-18, and KW-6.
The KW-6, Kluyveromyces marxiaus KW-6, is preserved in China Center for Type Culture Collection (CCTCC) in 6 months and 2 days 2020 with the preservation number of CCTCC NO: M2020173.
The Kluyveromyces KW-6 is obtained by separation and purification in the following way:
enrichment: taking 1ml of soy sauce from a soy sauce factory in Wuhan City of Hubei province of China, and performing gradient dilution to 1032ml of the diluted solution was added to 20ml of YPD liquid medium, followed by shaking culture at 30 ℃ and 180rpm for 24 hours.
Separation: diluting YPD culture medium to 10%-6-10-7And coating 100 mu L of diluted bacterial liquid on a YPD agar medium plate, and culturing at 30 ℃ for 24-48 h until bacterial colonies are formed.
And (3) purification: and picking a single bacterial colony which has a smooth, moist and viscous surface, is easy to pick up, has uniform bacterial colony texture and uniform color on the front side, the back side, the edge and the central part to obtain the Kluyveromyces KW-6 strain.
26S rDNA sequencing is carried out on Kluyveromyces KW-6, and NCBI-BLAST comparison is carried out on the sequencing result.
The primers used were: forward primer NL 1: 5'-GCATATCAATAAGCGGAGGAAAAG-3', respectively; reverse primer NL 4: 5'-GGTCCGTGTTTCAAGACGG-3' are provided.
And (3) PCR reaction system: 15 μ l Super Mix,1 μ l sample, 1 μ l NL 1; 1 μ l NL4,12 μ l ddH2O, 30 μ l total;
PCR reaction procedure: 5min at 96 ℃; 96 ℃ 20s, 56 ℃ 20s, 72 ℃ 30s (35 cycles); 10min at 72 ℃; at 16 ℃ forever. The PCR products were sent to the sequencing company for sequencing and the sequencing results were compared by NCBI-BLAST. The 26S rDNA gene sequence SEQ ID NO.1 of the obtained Kluyveromyces KW-6 is shown as follows:
ggaggaaaagaaaccaaccgggattgccttagtaacggcgagtgaagcggcaaaagctcaaatttgaaatctggcgtcttcgacgtccgagttgtaatttgaagaaggcgactttgtagctggtccttgtctatgttccttggaacaggacgtcatagagggtgagaatcccgtgtggcgaggatcccagttatttgtaaagtgctttcgacgagtcgagttgtttgggaatgcagctctaagtgggtggtaaattccatctaaagctaaatattggcgagagaccgatagcgaacaagtacagtgatggaaagatgaaaagaactttgaaaagagagtgaaaaagtacgtgaaattgttgaaagggaagggcatttgatcagacatggcgtttgcttcggctttcgctgggccagcatcagttttagcggttggataaatcctcgggaatgtggctctgcttcggtagagtgttatagcccgtgggaatacagccagctgggactgaggattgcgacttttgtcaaggatgctggcgtaatggttaaatgccgcccgtcttga
EXAMPLE 3 determination of the inhibitory Activity of Yeast fermentation broth supernatant on tyrosine aminotransferase
The tyrosinase inhibitory activity was measured on 10 yeast strains selected in example 2, which have high DPPH.radical and OH.radical scavenging rates.
Firstly, reagent preparation:
sodium phosphate buffer (pH 6.8): 1.000g of sodium dihydrogen phosphate and 1.186g of disodium hydrogen phosphate are accurately weighed, a small amount of deionized water is added for dissolution, the volume is determined to be 500mL, and the mixture is stored in a refrigerator at 4 ℃ for later use.
L-tyrosine solution (7.5 mmol/L): accurately weighing 0.136g of L-tyrosine, firstly adding a plurality of drops of concentrated hydrochloric acid, adding about 50mL of deionized water, heating at 90 ℃ to completely dissolve, then adjusting the pH to about 7 by using a sodium hydroxide solution, adding deionized water to a constant volume to a 100mL brown volumetric flask, and storing in a dark place.
Test solution: supernatant of fermentation broth prepared in EXAMPLE 2
Tyrosinase liquid: dissolving the solid powder enzyme reagent in a small packaging bottle by using distilled water, sucking out the solid powder enzyme reagent by using a rubber head straw, transferring the solid powder enzyme reagent into a 250ml volumetric flask, continuously repeating the dissolving and transferring processes until the sucked enzyme liquid is changed from yellow brown to clear and transparent, and finally fixing the volume of 250ml in the volumetric flask to obtain 100u/ml enzyme liquid. 250ml of enzyme solution is subpackaged by a small centrifuge tube and stored at-20 ℃. The test piece is taken out as required in the experimental process and is used after being unfrozen.
The detection method comprises the following steps:
the total reaction system was 5 mL. The specific reaction system reagent composition is shown in Table 4.
TABLE 45mL reaction System
In the experiment, phosphate buffer solution, test solutions of different strains and enzyme solution are sequentially added into a test tube, and water bath is carried out for 10min at 30 ℃. The substrate L-tyrosine was then added and the timer was started immediately. The absorbance at a wavelength of 475nm at 40min of the reaction was determined. During the determination, the inhibition rate of the test solution on tyrosinase is calculated by using the following formula with the corresponding negative control as a reference.
The inhibition rate was [ (A-B)/A ]. times.l 00%
Wherein, A is the light absorption value of the standard control, and B is the light absorption value of the test solution. Each experiment was done in 3 replicates. The high inhibition rate indicates that the inhibition intensity of the compound on tyrosinase activity is high. The measurement results are shown in Table 5.
TABLE 5
As can be seen from Table 5, the inhibition rate of Xuelian3208-3 on tyrosinase activity is highest in 10 yeast strains.
According to the test results of examples 2 and 3, it can be seen that the yeast Xuelian3208-3 has high clearance rate to DPPH free radical and OH free radical, and has highest inhibition rate to tyrosinase activity.
Example 4 Strain identification
The microzyme Xuelian3208-3 strain is inoculated on YPD culture medium for streak culture, and the colony morphology is observed. On YPD medium, the colony is milky white, round, slightly convex, 2.5-4 mm in size, smooth in surface, regular in edge, and opaque, and the colony morphology is as shown in FIG. 2.
A single colony was picked up and dissolved in 1ml of sterile water, and the cell morphology was observed under a microscope. The identification result shows that: the single colony was selected as yeast, the cells were round or oval, germinated on the top, with clearly visible vacuoles. The morphological characteristics of the strain are shown in figure 3.
The yeast strain Xuelian3208-3 was subjected to 26S rDNA sequencing using high fidelity enzymes and to an NCBI-BLAST alignment of the sequencing results.
The primers used were: forward primer NL 1: 5'-GCATATCAATAAGCGGAGGAAAAG-3', respectively; reverse primer NL 4: 5'-GGTCCGTGTTTCAAGACGG-3' are provided.
And (3) PCR reaction system: 15 μ l Super Mix,1 μ l sample, 1 μ l NL 1; 1 μ l NL4,12 μ l ddH2O, 30 μ l in total;
PCR reaction procedure: 5min at 96 ℃; 96 ℃ 20s, 56 ℃ 20s, 72 ℃ 30s (35 cycles); 10min at 72 ℃; at 16 ℃ forever. The PCR products were sent to the sequencing company for sequencing and the sequencing results were compared by NCBI-BLAST. The 26S rDNA gene sequence of the resulting yeast strain Xuelian3208-3 is shown in SEQ ID NO.2 below:
gaggaaaagaaaccaaccgggattgccttagtaacggcgagtgaagcggcaaaagctcaaatttgaaatctagtaccttcggtgctcgagttgtaatttgtagagggatactttggggccgttccttgtctatgttccttggaacaggacgtcatagagggtgagaatcccgtgtggcgaggagtgcggttctatgtaaagtgccttcgaagagtcgagttgtttgggaatgcagctctaagtgggtggtaaattccatctaaagctaaatattggcgagagaccgatagcgaacaagtacagtgatggaaagatgaaaagaactttgaaaagagagtgaaaaagtacgtgaaattgttgaaagggaagggcatttgatcagacatggtgttttgcgccctctgctccttgtgggtgggggaatctcgcagctcactgggccaacatcagttttggtggtcggataaatccgtaggaatgtggcttgcctcggcaagtgttatagcctgcgggaatacggccagctgggactgaggactgcgacttttgtcaaggatgttggcataatggttatatgccgcccgtcttgaa
and displaying according to the comparison result: yeast strain Xuelian3208-3 is a single spore Saccharomyces cerevisiae (Kazachstania uniispora). The yeast strain Xuelian3208-3 was named as Saccharomyces cerevisiae Xuelian3208-3 strain (Kazachstania unispora Xuelian3208-3) and deposited. The single-spore saccharomyces cerevisiae Xuelian3208-3 strain (Kazachstania unispora Xuelian3208-3) is preserved in China Center for Type Culture Collection (CCTCC) at 7 months and 2 days in 2021, and the preservation number is CCTCC NO: M2021812.
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Sequence listing
<110> Hubei Angel biological group Co., Ltd
<120> single-spore saccharomyces cerevisiae strain and application thereof
<160> 2
<170> SIPOSequenceListing 1.0
<210> 1
<211> 564
<212> DNA
<213> Kluyveromyces marxiaus (Kluyveromyces marxiaus)
<400> 1
ggaggaaaag aaaccaaccg ggattgcctt agtaacggcg agtgaagcgg caaaagctca 60
aatttgaaat ctggcgtctt cgacgtccga gttgtaattt gaagaaggcg actttgtagc 120
tggtccttgt ctatgttcct tggaacagga cgtcatagag ggtgagaatc ccgtgtggcg 180
aggatcccag ttatttgtaa agtgctttcg acgagtcgag ttgtttggga atgcagctct 240
aagtgggtgg taaattccat ctaaagctaa atattggcga gagaccgata gcgaacaagt 300
acagtgatgg aaagatgaaa agaactttga aaagagagtg aaaaagtacg tgaaattgtt 360
gaaagggaag ggcatttgat cagacatggc gtttgcttcg gctttcgctg ggccagcatc 420
agttttagcg gttggataaa tcctcgggaa tgtggctctg cttcggtaga gtgttatagc 480
ccgtgggaat acagccagct gggactgagg attgcgactt ttgtcaagga tgctggcgta 540
atggttaaat gccgcccgtc ttga 564
<210> 2
<211> 591
<212> DNA
<213> Saccharomyces cerevisiae monospora (Kazachstania unespora)
<400> 2
gaggaaaaga aaccaaccgg gattgcctta gtaacggcga gtgaagcggc aaaagctcaa 60
atttgaaatc tagtaccttc ggtgctcgag ttgtaatttg tagagggata ctttggggcc 120
gttccttgtc tatgttcctt ggaacaggac gtcatagagg gtgagaatcc cgtgtggcga 180
ggagtgcggt tctatgtaaa gtgccttcga agagtcgagt tgtttgggaa tgcagctcta 240
agtgggtggt aaattccatc taaagctaaa tattggcgag agaccgatag cgaacaagta 300
cagtgatgga aagatgaaaa gaactttgaa aagagagtga aaaagtacgt gaaattgttg 360
aaagggaagg gcatttgatc agacatggtg ttttgcgccc tctgctcctt gtgggtgggg 420
gaatctcgca gctcactggg ccaacatcag ttttggtggt cggataaatc cgtaggaatg 480
tggcttgcct cggcaagtgt tatagcctgc gggaatacgg ccagctggga ctgaggactg 540
cgacttttgt caaggatgtt ggcataatgg ttatatgccg cccgtcttga a 591
Claims (12)
1. A single-spore saccharomyces cerevisiae strain is a single-spore saccharomyces cerevisiae Xuelian3208-3 strain (Kazachstania unispora Xuelian3208-3), which is preserved in China Center for Type Culture Collection (CCTCC) at 7-2 months in 2021, and the preservation number is M2021812.
2. The strain of s.cerevisiae monospora according to claim 1, wherein the 26S rDNA gene sequence of the strain of s.cerevisiae Xuelian3208-3 is as shown in SEQ ID No. 2.
3. A fermentation preparation method of a single-spore saccharomyces cerevisiae microbial inoculum is characterized by comprising the following steps: culturing the strain of s.cerevisiae monospora according to claim 1 or 2.
4. The method of claim 3, comprising the steps of:
(1) amplifying and culturing the saccharomyces cerevisiae monospora strain of claim 1 or 2;
(2) adding the product obtained in the step (1) into a liquid culture medium, and fermenting and culturing at the temperature of 26-32 ℃.
5. A microbial inoculum obtained by the fermentation production method according to claim 3 or 4.
6. A s.cerevisiae strain of monospora according to claim 1 or 2 or a fermentation product obtained by fermentation of the microbial agent according to claim 5.
7. The fermentation product of claim 6, wherein the fermentation product has free radical scavenging ability and/or tyrosinase inhibitory activity.
8. The fermentation product of claim 6, wherein the fermentation product has a DPPH-free radical clearance of greater than 95%;
alternatively, preferably, the fermentation product has a clearance of OH radicals of greater than 80%;
alternatively, preferably, the fermentation product has greater than 88% inhibition of tyrosinase activity.
9. The method for producing a fermentation product according to any one of claims 6 to 8, which comprises culturing the Saccharomyces cerevisiae strain according to claim 1 or 2 or the microbial preparation according to claim 5.
10. The method of claim 9, comprising the steps of:
(1) amplifying and culturing the saccharomyces cerevisiae monospora strain of claim 1 or 2 or the microbial inoculum of claim 5;
(2) adding the product obtained in the step (1) into a liquid culture medium, and fermenting and culturing at 26-32 ℃ to obtain the fermentation product.
11. Use of the strain of saccharomyces cerevisiae monospora according to claim 1 or 2 or the microbial inoculum according to claim 5 in antioxidant, whitening anti-aging or anti-aging products.
12. Use according to claim 11, wherein the product is a food product, a cosmetic product or a nutraceutical product.
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| CN102286397A (en) * | 2011-06-03 | 2011-12-21 | 新疆医科大学 | Polymicrobic fermentation agent and polymicrobic fermentation milk product for preventing atherosclerosis and production method thereof |
| CN111088174A (en) * | 2019-12-30 | 2020-05-01 | 上海应用技术大学 | Saccharomycetes Fungiensis with oxidation resistance and whitening effect and application thereof |
| CN111471604A (en) * | 2020-04-20 | 2020-07-31 | 中国农业科学院麻类研究所 | Application of saccharomyces cerevisiae Z L G-6 and lactobacillus plantarum Picp-2 |
| JP2021147380A (en) * | 2020-03-13 | 2021-09-27 | 株式会社フィス | Antioxidant composition |
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| CN102286397A (en) * | 2011-06-03 | 2011-12-21 | 新疆医科大学 | Polymicrobic fermentation agent and polymicrobic fermentation milk product for preventing atherosclerosis and production method thereof |
| CN111088174A (en) * | 2019-12-30 | 2020-05-01 | 上海应用技术大学 | Saccharomycetes Fungiensis with oxidation resistance and whitening effect and application thereof |
| JP2021147380A (en) * | 2020-03-13 | 2021-09-27 | 株式会社フィス | Antioxidant composition |
| CN111471604A (en) * | 2020-04-20 | 2020-07-31 | 中国农业科学院麻类研究所 | Application of saccharomyces cerevisiae Z L G-6 and lactobacillus plantarum Picp-2 |
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