CN113907352A - Health-care product composition for enhancing immunity and preparation method and application thereof - Google Patents
Health-care product composition for enhancing immunity and preparation method and application thereof Download PDFInfo
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Abstract
The invention discloses a health-care product composition for enhancing immunity, a preparation method and application thereof, wherein the health-care product composition is prepared from the following raw material medicines in parts by weight: the health-care product composition can be prepared into any conventional oral preparation form with or without medicinal auxiliary materials, is obtained according to the traditional Chinese medicine dialectical theory and according to the principle of strengthening body resistance and eliminating pathogenic factors of the traditional Chinese medicine, animal experiments and multiple prescription screening, has anti-allergic reaction, oxidation stress and injury resistance and anti-allergic reaction, and can play a role in enhancing the immunity of the organism after being taken for a long time.
Description
Technical Field
The invention relates to a health food and a preparation method thereof, in particular to a health food for enhancing immunity and a preparation method thereof.
Background
Immunity is the body's own defense mechanism, and is the body's ability to recognize and destroy any foreign body (virus, bacteria, etc.) that invades from the outside, to treat aged, damaged, dead, denatured self cells, and to recognize and treat mutant cells and virus-infected cells in the body.
In the modern society, the sharp increase of working and living pressure is accompanied by environmental and dietary pollution, so that the immunity of the organism is sharply reduced, and the organism is in subclinical symptoms, such as tiredness, continuous cold, easy infection, inappetence, easy relapse of old diseases and the like, and even cardiovascular diseases, diabetes and tumor diseases occur. Therefore, the health care food has the advantages of keeping good mood, balancing diet, doing proper exercise and performing proper nourishment, thereby being of great importance for improving the immunity.
Because the autoimmune system can prevent, inhibit and eliminate the invaded virus, the most effective medicine is the immunity of the human body, so that the research and development of health-care food for enhancing the immunity have important clinical significance.
Researches find that people are in a sub-health state at present, and a single raw material component can only control or relieve certain symptoms and cannot fundamentally solve the complicated physical conditions caused by low immunity. The traditional Chinese medicine has wide sources, less adverse reactions and difficult generation of drug resistance, has unique effects on coordinating human body balance and enhancing the disease resistance of organisms, and therefore has important research significance for researching and developing traditional Chinese medicine health-care food for enhancing immunity.
The research discovers that the traditional Chinese medicine health food is prepared from medlar, Chinese date, fructus cannabis, rhizoma polygonati, dandelion and astragalus membranaceus, and has the characteristics of enhancing the medicine effect, reducing toxic and side effects and the like. Researches find that the medlar has the functions of improving disease resistance, protecting liver, tonifying kidney, enriching blood, tonifying qi and the like; the Chinese date has the functions of nursing viscera, enriching blood, strengthening brain, invigorating spleen and the like; fructus Cannabis has effects of loosening bowel to relieve constipation, tonifying deficiency, and repairing myocardium; rhizoma Polygonati has effects of nourishing yin, moistening lung, invigorating spleen qi, and replenishing kidney essence; the dandelion has the functions of clearing away heat and toxic materials, removing stasis, inducing diuresis and treating stranguria; radix astragali has effects of relieving swelling, arresting sweating, and resisting infection.
Through the research on the half hemolysis value (HC50), splenocyte proliferation, transformation capability and delayed allergy in mouse serum, the influence of the health food on the immunity of mice is observed, and the research finds that the health food can improve the half hemolysis value (HC50) of mice, promote the lymphocyte proliferation and transformation capability, enhance the delayed allergy of the mice and have the function of enhancing the immunity.
Disclosure of Invention
The invention aims to provide a health-care medicine or health-care food or health-care product for enhancing immunity, which can enhance immunity, reduce toxic and side effects, prolong the life of a patient, improve the life quality of the patient, has good safety and low price, and is convenient to transport and store.
Therefore, the invention provides a health-care product composition for enhancing immunity, which is characterized by being prepared from the following traditional Chinese medicine raw materials: medlar, Chinese date, fructus cannabis, rhizoma polygonati, dandelion and astragalus membranaceus.
Through screening and optimization, the health-care product composition is prepared from the following raw material medicines in parts by weight: the Chinese wolfberry fruit-fructus lycii, the Chinese date, the fructus cannabis, the rhizoma polygonati, the dandelion and the radix astragali are mixed according to the weight ratio of 2:1:2:1:1: 3.
The health-care product composition is characterized in that: with or without pharmaceutical excipients.
The health-care product composition is characterized in that: the composition can be made into any dosage form for administration.
The health-care product composition is characterized in that: the dosage form is an oral dosage form.
The health-care product composition is characterized in that: the oral dosage form is selected from: tablet, capsule, oral liquid, granule, powder, and pill.
The invention further provides a preparation method of the health care product composition, which is characterized by comprising the following steps: the method comprises the following steps: mixing fructus Lycii, fructus Jujubae, fructus Cannabis, rhizoma Polygonati, herba Taraxaci, and radix astragali at a certain ratio, decocting with water for 2-4 hr, and filtering with filter paper or ultrafiltration membrane to obtain extract. The extract can be further used as an active substance to prepare a preparation composition according to the conventional technology of pharmaceutical science.
The health-care product composition is applied to preparing health-care medicines or health-care foods for improving immunity, wherein the function of improving immunity is to improve cellular immunity and humoral immunity.
In modern society, many diseases are caused by low immunity or poor immunity. Aiming at the etiology and pathogenesis of hypoimmunity, the inventor develops a health food for enhancing immunity by taking the traditional Chinese medicine theory as the basis and combining the clinical practice of the traditional Chinese medicine.
Compared with the prior art, the invention has the beneficial technical effects that:
1. the screening proves that the extract prepared from the traditional Chinese medicine raw materials in a specific weight proportion has the most remarkable effect of enhancing the immunity, and the other proportions have poorer effects than the traditional Chinese medicine raw materials in the invention, which is found by experiments.
2. Tests show that the health food can obviously improve half of hemolytic value (HC50) of mice, promote lymphocyte proliferation and transformation capability and enhance delayed type allergic reaction of the mice, and other proportions are all poorer than the effects of the health food, so the health food can be used as a safe and effective immunity enhancing medicine or health food.
3. The safety is good: the health food of the present invention has great tolerance amount and no obvious toxic side effect.
4. The medicine is simple and convenient to take, and is easy to be absorbed by human or animals when orally taken.
5. The raw materials of the medicine of the invention are medlar, Chinese date, fructus cannabis, rhizoma polygonati, dandelion and astragalus, compared with other imported immunity-enhancing medicines, the price is low, the cost performance is high, and the medicine is easy to be accepted by patients.
6. Is convenient for transportation and storage, sealed, and placed in a cool and dry place.
Drawings
FIG. 1 shows the effect of the health food of the present invention on the ConA-induced spleen lymphocyte transformation in mice. Compared with a blank control group, the OD difference of the health-care food of the low dose (0.13mg/g/d) and the high dose (0.65mg/g/d) is obviously increased, P is less than 0.05vs. the blank control group, and n is 6-11.
FIG. 2. Effect of the health food of the present invention on delayed allergy (DTH) in mice. Compared with the blank control group, the OD difference values of the low dose group (0.13mg/g/d) and the high dose group (0.65mg/g/d) are obviously increased, P is less than 0.05vs. the blank control, P is less than 0.001vs. the blank control, and n is 10-15.
FIG. 3 shows the half-maximal hemolysis value (HC) of mice treated with the health food of the present invention50). Low dose (0.1) compared to blank controlThe OD difference of 3mg/g/d) and high dose group (0.65mg/g/d) is obviously increased, and P is<0.05vs. blank control, P<0.01vs. blank, n-10-14.
Detailed Description
The invention is further illustrated by the following examples.
Example 1
Preparing an extract:
taking medlar, Chinese date, fructus cannabis, rhizoma polygonati, dandelion and astragalus mongholicus according to the weight ratio of 2:1:2:1:1:3, adding 10 times of water, decocting for 3 hours to obtain a water decoction, concentrating and filtering to obtain a water extract.
Example 2
Preparation of tablets:
tablets are prepared according to the conventional technology of pharmaceutical science by taking the extract of example 1 as an active substance.
Example 3
Preparation of capsules:
capsules were prepared according to conventional techniques of pharmaceutics using the extract of example 1 as the active substance.
Example 4
Preparing oral liquid:
the extract of example 1 was used as an active substance, and prepared into an oral liquid according to a conventional technique of pharmaceutical science.
Example 5
Preparation of granules:
the extract of example 1 was used as an active substance, and prepared into granules according to a conventional technique of pharmaceutical formulation.
Example 6
Preparation of the pill:
the extract of example 1 was used as an active substance, and prepared into pills according to a conventional technique of pharmaceutical formulation.
Efficacy testing of the products of the invention
1. Experimental materials:
1.1 experimental grouping: blank control group, low dose group health food (0.13mg/g/d), high dose group health food (0.65mg/g/d)
1.2 main drugs: 1640 medium (Gibco); fetal bovine serum (Hyclone), D-hanks water; an MTT kit; a Du's reagent; concanavalin a (cona); packed Sheep Red Blood Cells (SRBC); guinea pig serum; hydrochloric acid; isopropyl alcohol; SA buffer solution; middle and low dosage Chinese medicinal compound extract; high-dose Chinese medicinal compound extract
1.3 Main instruments: the main apparatus is as follows: an enzyme-labeling instrument; a cell counter; a microscope; a carbon dioxide incubator; a vernier caliper; water bath pot
1.4 preparation of health food:
mixing fructus Lycii, fructus Jujubae, fructus Cannabis, rhizoma Polygonati, herba Taraxaci, and radix astragali at a ratio of 2:1:2:1:1:3, decocting with water for 2-4 hr, and filtering with filter paper or ultrafiltration membrane to obtain extract.
2. The experimental method comprises the following steps:
2.1 Experimental animals and groups: 100 healthy Kunming mice (male and female) of SPF grade, weighing 18-22g, were provided by the laboratory animal center of Harbin medical university. Group I mice lymphocyte transformation experiment induced by ConA; group II delayed allergy test; group III hemolytic value experiments were performed.
2.2 dose selection and subject administration: the health food is calculated by 70kg of adults, the dosage is 1mg/kg and 5mg/kg per day, the dosage converted into the mouse is 130mg/kg and 650mg/kg respectively, the animals with the corresponding dosages are respectively subjected to intragastric administration, the intragastric administration volume is 0.2mL/10g BW, the negative control group is subjected to the administration of pure water with the same volume, the intragastric administration is carried out for 1 time per day, and the intragastric administration is continuously carried out for 30 days.
Experimental example 1: the influence of the health care product on the cellular immunity of mice
ConA-induced splenic lymphocyte transformation assay (MTT method) in mice
Subjecting the tested mouse to cervical dislocation, aseptically taking spleen, placing in sterile Hank's solution to obtain single cell suspension, filtering with 200 mesh screen, washing with Hank's solution for three times, and regulating cell concentration to 1 × 10 with RPMI1640 complete culture solution6one/mL. The cell suspension was added to a 24-well plate in two wells, 1ml per well, 75. mu.l ConA solution (equivalent to 7.5. mu.g/ml) was added to one well, and the other well was used as a control. Culturing in 5% CO2, carbon dioxide incubator at 37 deg.C for 72 h. CulturingAfter 4 hours before the end, 0.7ml of the supernatant was gently aspirated from each well, 0.7ml of calf serum-free RPMI1640 culture medium was added thereto together with 50. mu.l/well of MTT (5mg/ml), and the culture was continued for 4 hours. After the culture is finished, adding 1ml of acidic isopropanol into each hole, blowing, beating and uniformly mixing to ensure that the purple crystals are completely dissolved. The cells were transferred to a 96-well plate, and each well was divided into 3 wells as parallel control wells, and the OD value was measured at a wavelength of 570nm using a microplate reader, and the OD value of the wells with ConA was finally subtracted from the OD value of the wells without ConA to thereby indicate the proliferative capacity of lymphocytes.
The results are shown in FIG. 1: after 30 days of continuous gavage administration, the lymphocyte transformation capacity of the mice with the health-care food of the low-dose group and the high-dose group is higher than that of the mice with the blank control group, and the mice with the health-care food of the low-dose group and the high-dose group have obvious statistical difference (P <0,05) compared with the mice with the blank control group, which shows that the health-care food has the functions of promoting the lymphocyte proliferation and transformation capacity.
2. Delayed allergic reaction (DTH) assay in mice
The left hind foot plantar thickness was measured 4 days after immunization by intraperitoneal injection of 2% (v/v) SRBC, 0.2mL per mouse, 20uL per mouse by subcutaneous injection of 20% (v/v) SRBC at the measurement site, 24h after injection, three times at the same site, and the mean value was taken.
The results are shown in FIG. 2: after the administration is continuously carried out for 30 days, the difference (mm) of the swelling degrees of the foot metatarsal swelling of the mice before and after SRBC injection is measured through a delayed allergy reaction experiment, compared with a blank control group, the swelling degrees of the foot metatarsal swelling of the mice can be enhanced by the health food of the low-dose group and the high-dose group, and the statistical difference (P <0,05) is realized, so that the health food can enhance the delayed allergy of the mice and has the function of enhancing the immunity.
Experimental example 2: effect of the health product of the present invention on humoral immunity of mice
3. Half maximal hemolytic value (HC) of mice50) Experiment of
Each mouse was injected intraperitoneally with 1mL of a 2% SRBC cell suspension. On the 4 th day after immunization, the eyeball is removed to draw blood, the blood is fully separated after standing for 1h, the blood is centrifuged at 3000rpm for 10min, and the blood serum is collected. The serum was diluted 200-fold with SA buffer, 100uL of the diluted serum was taken, 10% SRBC 50. mu.l and complement 100. mu.l were sequentially added (diluted 1:8 with SA buffer), and incubated in a 37 ℃ thermostatic water bath for 30min, followed by centrifugation at 1500rpm for 10 min. 50. mu.l of the supernatant was added to a 96-well plate, and 150. mu.l of Dow's reagent was added thereto. Setting half number of hemolysis holes, standing for 10min, measuring optical density value of each hole at 540nm with full-automatic enzyme calibration instrument, and calculating hemolysin quantity according to the following formula, wherein the hemolysin quantity is expressed by half number of hemolysis value (HC 50):
sample HC50 ═ x dilution factor [ (sample optical density value-blank optical density value)/(optical density value-blank optical density value at SRBC half hemolysis) ] x
The results are shown in FIG. 3: HC determination by serum hemolysin assay50The low dose group health food and the high dose group health food increased the half-maximal hemolysis value of mice dose-dependently, both with statistical difference (P) compared to the placebo group<0.05) shows that the health food can improve half of hemolysis value of mice.
The results show that the health food disclosed by the invention has the effects of promoting the proliferation and transformation of lymphocytes, enhancing the delayed type allergic reaction of mice, improving the half hemolysis value (HC50) of the mice and enhancing the immunity according to the principle of strengthening the body resistance and eliminating pathogenic factors in traditional Chinese medicine and animal experiments, and is expected to be developed into a novel, safe and effective traditional Chinese medicine health product for enhancing the immunity.
The above embodiments are merely exemplary and do not limit the scope of the present invention. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention, and that such changes and modifications may be made without departing from the spirit and scope of the invention.
Claims (7)
1. The health-care product composition for enhancing immunity is characterized by being prepared from the following raw material medicines in parts by weight: the Chinese wolfberry fruit-fructus lycii, the Chinese date, the fructus cannabis, the rhizoma polygonati, the dandelion and the radix astragali are mixed according to the weight ratio of 2:1:2:1:1: 3.
2. The nutraceutical composition of claim 1, wherein: with or without pharmaceutical excipients.
3. The nutraceutical composition of claim 1, wherein: the composition can be made into any dosage form for administration.
4. The nutraceutical composition of claim 3, wherein: the dosage form is an oral dosage form.
5. The nutraceutical composition of claim 4, wherein: the oral dosage form is selected from: tablet, capsule, oral liquid, granule, powder, and pill.
6. A process for preparing a nutraceutical composition as in claim 1, wherein: the method comprises the following steps: mixing fructus Lycii, fructus Jujubae, fructus Cannabis, rhizoma Polygonati, herba Taraxaci, and radix astragali at a certain ratio, decocting with water for 2-4 hr, and filtering with filter paper or ultrafiltration membrane to obtain extract.
7. The use of the nutraceutical composition of claim 1 for the preparation of a nutraceutical or health food for enhancing immunity, which is enhancing cellular and humoral immune functions.
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