CN113907094B - Ultraviolet sterilization synergist-France cypress essential oil and application thereof - Google Patents
Ultraviolet sterilization synergist-France cypress essential oil and application thereof Download PDFInfo
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- CN113907094B CN113907094B CN202111154376.4A CN202111154376A CN113907094B CN 113907094 B CN113907094 B CN 113907094B CN 202111154376 A CN202111154376 A CN 202111154376A CN 113907094 B CN113907094 B CN 113907094B
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- 239000000341 volatile oil Substances 0.000 title claims abstract description 60
- 230000001954 sterilising effect Effects 0.000 title claims abstract description 51
- 241000218691 Cupressaceae Species 0.000 title claims abstract description 43
- 238000004659 sterilization and disinfection Methods 0.000 title abstract description 42
- 238000000034 method Methods 0.000 claims abstract description 14
- 230000001580 bacterial effect Effects 0.000 claims description 24
- 239000007788 liquid Substances 0.000 claims description 17
- 230000001678 irradiating effect Effects 0.000 claims description 6
- 244000301850 Cupressus sempervirens Species 0.000 claims description 3
- 238000009281 ultraviolet germicidal irradiation Methods 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 2
- 241000218636 Thuja Species 0.000 claims 1
- 241000894006 Bacteria Species 0.000 abstract description 17
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- CRPUJAZIXJMDBK-UHFFFAOYSA-N camphene Chemical compound C1CC2C(=C)C(C)(C)C1C2 CRPUJAZIXJMDBK-UHFFFAOYSA-N 0.000 description 2
- SVURIXNDRWRAFU-OGMFBOKVSA-N cedrol Chemical compound C1[C@]23[C@H](C)CC[C@H]3C(C)(C)[C@@H]1[C@@](O)(C)CC2 SVURIXNDRWRAFU-OGMFBOKVSA-N 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
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- PXRCIOIWVGAZEP-UHFFFAOYSA-N Primaeres Camphenhydrat Natural products C1CC2C(O)(C)C(C)(C)C1C2 PXRCIOIWVGAZEP-UHFFFAOYSA-N 0.000 description 1
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- 229930006739 camphene Natural products 0.000 description 1
- ZYPYEBYNXWUCEA-UHFFFAOYSA-N camphenilone Natural products C1CC2C(=O)C(C)(C)C1C2 ZYPYEBYNXWUCEA-UHFFFAOYSA-N 0.000 description 1
- 229940026455 cedrol Drugs 0.000 description 1
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- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 230000000749 insecticidal effect Effects 0.000 description 1
- SVURIXNDRWRAFU-UHFFFAOYSA-N juniperanol Natural products C1C23C(C)CCC3C(C)(C)C1C(O)(C)CC2 SVURIXNDRWRAFU-UHFFFAOYSA-N 0.000 description 1
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- 230000002936 tranquilizing effect Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/06—Coniferophyta [gymnosperms], e.g. cypress
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/02—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using physical phenomena
- A61L2/08—Radiation
- A61L2/10—Ultraviolet radiation
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
The invention belongs to the technical field of ultraviolet sterilization, and discloses an ultraviolet sterilization synergist and a method for sterilizing by combining ultraviolet sterilization synergist with ultraviolet. The research is based on that the Ultraviolet (UVA) of the A wave band and the cypress essential oil of French has weak sterilization effect (the sterilization amount is 0.5log-1.5log when the Ultraviolet (UVA) and the cypress essential oil of French act independently), and the Ultraviolet (UVA) and the cypress essential oil of French act together, so that the cypress essential oil of French has obvious synergistic sterilization effect. Compared with the traditional ultraviolet sterilization technology, the ultraviolet sterilization device can kill bacteria more efficiently and reduce the influence of the quality of the ultraviolet lamp on the sterilization effect.
Description
Technical Field
The invention relates to the technical field of ultraviolet sterilization, and particularly relates to an ultraviolet sterilization synergist of cypress essential oil of France and application thereof.
Background
The ultraviolet sterilization technique is a convenient method with no chemical residue and little environmental impact, and is commonly used for sterilizing gas, liquid and solid surfaces. Ultraviolet radiation is a generic term for radiation in the electromagnetic spectrum having a wavelength of 10nm to 400 nm. It is invisible light with a higher frequency than blue-violet light, and ultraviolet rays are classified into UVA, UVB, UVC, and UVD. The ultraviolet lamp sterilization is used in various places due to the advantages of low cost, convenient use, no drug resistance and the like. In recent years, bacterial drug resistance is getting worse due to the use of a large amount of antibiotics, and the effect of treating infection caused by germs is gradually weakened along with the appearance of multi-drug resistant bacteria, so that the research on non-antibiotic sterilization technology is more and more applied in clinic, and the research on ultraviolet sterilization effect is more and more intensive.
The principle of the ultraviolet sterilization technology is that ultraviolet radiation with proper wavelength is utilized to destroy the molecular structure of DNA (deoxyribonucleic acid) or RNA (ribonucleic acid) in organism cells to cause death of growing cells and/or regenerating cells, thereby achieving the sterilization effect. The ultraviolet sterilization technology is based on modern epidemic prevention science, medicine and photodynamic, and specially designed ultraviolet with high efficiency, high strength and long service life is adopted to irradiate the surface of an object to directly kill various pathogens such as bacteria, viruses, parasites, algae and the like on the surface of the object. Meanwhile, bacteria can be killed under the condition that antibiotic resistance is not generated, so that fine drug resistance is not improved. However, low-energy uv alone is not sufficient to achieve a very good bactericidal effect, the sterilization time is long and the bacteria cannot be completely killed.
The essential oil of France cypress (cypress) is prepared by distilling fresh leaves and cones. Colorless or very pale yellow, with an amber taste that is woody and balsamic. The essential oil mainly comprises camphene, cedrol, cedar camphor, etc., and has antibacterial, insecticidal, and tranquilizing effects. In order to enhance the ultraviolet sterilization effect, the research of the ultraviolet sterilization effect synergist is an essential step. However, the currently reported essential oil still has less ultraviolet sterilization synergistic effect, and a new ultraviolet sterilization synergistic agent needs to be supplemented urgently to increase the sterilization effect of ultraviolet rays in various occasions.
Disclosure of Invention
The invention aims to overcome the defects in the prior art and provide the application of the French cypress essential oil as the ultraviolet sterilization synergist.
The second purpose of the invention is to provide a method for sterilizing by using the French cypress essential oil in combination with ultraviolet.
The purpose of the invention is realized by the following technical scheme:
the application of the French cypress essential oil as an ultraviolet sterilization synergist.
The research of the invention finds that the combination of the French cypress essential oil and ultraviolet can obviously enhance the ultraviolet sterilization capability, so that the French cypress essential oil can be used as a synergist for ultraviolet sterilization.
The invention also provides an ultraviolet sterilization synergist which comprises the French cypress essential oil.
The invention also provides a method for sterilizing by combining ultraviolet with the French cypress essential oil, wherein the irradiation time of the ultraviolet is 30min, and the concentration of the French cypress essential oil is 1%.
Preferably, in the method for sterilizing, the ultraviolet intensity is 2.4-3.0mW/cm 2 。
Preferably, in the sterilization method, the ultraviolet irradiation is performed for 30min before preheating.
Preferably, the method of sterilizing comprises the steps of:
(1) Mixing the bacterial liquid with 1% of French cypress essential oil, placing the mixture in a six-hole plate, and setting ultraviolet irradiation conditions: wavelength range of 300-460nm, power of 18W, distance from six-hole plate to ultraviolet lamp tube of 8cm, ultraviolet intensity of 2.4-3.0mW/cm 2 ；
(2) Preheating the ultraviolet box for 20-40min, and placing the six-hole plate in the ultraviolet box for irradiating for 25-35min.
More preferably, the final concentration of the bacterial liquid in the step (1) of the sterilization method is 10 6 CFU/mL。
Compared with the prior art, the invention has the following beneficial effects:
the invention provides a synergist having a synergistic effect with ultraviolet, and the research is based on that the sterilization effect of Ultraviolet (UVA) in the A wave band and the sterilization effect of the cypress essential oil in French is weak (the sterilization amount is 0.5log-1.5log when the Ultraviolet (UVA) and the cypress essential oil act independently), and the synergistic sterilization effect is obvious after the Ultraviolet (UVA) and the cypress essential oil are combined to act. Compared with the traditional ultraviolet sterilization technology, the ultraviolet sterilization device can kill bacteria more efficiently and reduce the influence of the quality of the ultraviolet lamp on the sterilization effect.
Detailed Description
The following further describes embodiments of the present invention. It should be noted that the description of the embodiments is provided to help understanding of the present invention, but the present invention is not limited thereto. In addition, the technical features involved in the embodiments of the present invention described below may be combined with each other as long as they do not conflict with each other.
The test methods used in the following experimental examples are all conventional methods unless otherwise specified; the materials, reagents and the like used are, unless otherwise specified, commercially available reagents and materials.
The ultraviolet lamp used is PHILIPS TL-D brand; wattage: 18W; voltage: 220V; wavelength: (UVA) 300-469nm; tube diameter of the lamp tube: 25mm; length: 60cm. The French cypress essential oil is of the Satya brand (Italy).
The judgment principle is as follows: for example, the bacterial quantity reduced by the experimental group is reduced by 2 log values on the basis of the bacterial quantity reduction of the ultraviolet treatment alone and the essential oil treatment alone, and the essential oil and the ultraviolet have the synergistic sterilization effect.
Example 1 germicidal Effect of different UV irradiation times on ATCC25922 Strain
1. Experimental materials:
(1) The ultraviolet lamp used for the test is PHILIPS TL-D brand; wattage: 18W; voltage: 220V; wavelength: (UVA) 300-469nm; tube diameter of the lamp tube: 25mm; length: 60cm.
(2) Test medium: the MH agar medium and the MacConkey agar medium (purchased from Guangdong Huancao Microscience Co., ltd.) which were sterilized by autoclaving were cooled to 55 ℃, 20mL of the MH agar medium and the MacConkey agar medium were put into a sterile petri dish by a pipette, and naturally dried for 30min to prepare the MH agar medium and the MacConkey agar medium.
Coli standard strain ATCC25922 (laboratory collection).
2. Preparation work before the test:
(1) Starting an ultraviolet lamp and continuously irradiating for 30 minutes for preheating;
(2) The E.coli standard strain ATCC25922 was cultured on MacconyKa medium to a suitable size.
3. Ultraviolet sterilization effect evaluation experiment:
(1) Inoculating Escherichia coli ATCC25922, placing single colony in a centrifuge tube filled with 4mL MH broth, putting the centrifuge tube in a 37-degree shaking table, incubating for 4 hours at 180 revolutions, and taking out the centrifuge tube;
(2) Placing the centrifuge tube in a centrifuge, centrifuging for 8min at 5000 rpm, pouring out the supernatant, adding equal volume of normal saline for resuspension, and performing gradient dilution to obtain final bacterial load of 10 6 CFU/mL；
(3) Adding 1mL of bacterial liquid into a six-hole plate;
(4) Setting blank control group and ultraviolet irradiation treatment group, wherein the ultraviolet irradiation treatment group is divided into three groups, and irradiating for 15min, 30min and 60min respectively.
(5) After the ultraviolet irradiation is finished, 100 mu L of bacterial liquid is absorbed and added into a 2mL centrifuge tube filled with 900 mu L of 0.85% physiological saline for gradient dilution, 25 mu L of bacterial liquid is absorbed and dropped on an MH agar culture medium after dilution, the culture box with the temperature of 37 ℃ is incubated for 16-18h, counting is carried out, and the statistical analysis is carried out after the experimental result is repeated through three biology.
The results are shown in table 1, the bacteriostatic effect of ultraviolet on bacteria is increased with the increase of the irradiation time, wherein the difference between the number of bacteria in the group irradiated with ultraviolet for 15min and the number of bacteria in the blank control group is not large, which indicates that the short irradiation time of ultraviolet cannot produce a significant bacteriostatic effect on ATCC 25922. The number of bacteria in the group irradiated by ultraviolet for 30min begins to decrease, which indicates that the ultraviolet begins to have a certain bacteriostatic effect on the bacteria.
TABLE 1 germicidal Effect of UV irradiation time on ATCC25922 strains
Example 2 determination of MIC of different concentrations of Selaginella France essential oil against ATCC25922 Strain
1. Experimental materials:
(1) And (3) testing: autoclaved MH broth was cooled for use.
(2) Resin azure: macklin (Maclin) brand, MW 251.17, purity 90%;
coli standard strain ATCC25922 (laboratory collection).
2. Preparation work before the test:
(1) Taking out the French cypress essential oil from the refrigerator, balancing to room temperature, and sucking part of the French cypress essential oil for later use;
(2) The E.coli standard strain ATCC25922 was cultured on MacconyKa agar medium to a suitable size.
(3) Weighing 0.2512g of resin azure powder into a centrifuge tube, and adding 10mL of pure water for dissolving, wherein the concentration of the resin azure solution is 10mM/L.
3. Experiment for evaluating bactericidal effect of France cypress essential oil:
(1) Inoculating Escherichia coli ATCC25922, placing a single colony in a centrifuge tube filled with 4mL MH broth, putting the centrifuge tube in a 37-degree shaking table, incubating for 4 hours at 180 revolutions, and taking out the centrifuge tube;
(2) The incubated E.coli was diluted 100-fold using MH broth to about 10 6 CFU/mL for standby;
(3) Taking a sterile 96-well plate, adding 180 mu L of MH broth medium to the 1 st well, and adding 100 mu L of MH broth medium to the 2 nd-11 th well;
(4) Adding 20 mu L of essential oil with the original concentration into the 1 st hole, sucking 100 mu L to the 2 nd hole after blowing and beating uniformly, and repeating the steps, sucking 100 mu L from the 10 th hole and discarding; the concentration of the essential oil in each well is 10%, 5%, 2.5%, 1.25%, 0.625%, 0.3125%, 0.156%, 0.078%, 0.039%, 0.0195%.
(5) Adding 100 mu L of diluted bacteria solution into the 1 st to 10 th holes, wherein the concentration of essential oil in each hole is 5%, 2.5%, 1.25%, 0.625%, 0.3125%, 0.156%, 0.078%, 0.039%, 0.0195% and 0.01%, adding 100 mu L of bacteria solution into the 11 th hole, and adding 200 mu LMH broth into the 12 th hole;
(6) Repeating the steps (3) to (5) for three times;
(7) And putting the inoculated 96-well plate into a 37-degree incubator for incubation for 16-18h, sucking 10 mu L of 10mM/L resin azure with the concentration of 0.1mM/L, adding the resin azure into the hole, incubating for 2h, and reading the result.
The results are shown in Table 2, wherein the MIC value of the essential oil of Cupressus French is 0.313%, the bacteria are inhibited from growing at the concentration, and the final concentration of the essential oil of Cupressus French is 1% as the use concentration of the ultraviolet synergist.
TABLE 2 MIC of Selaginella France essential oil against ATCC25922 strain and concentrations selected for the experiment
| Experimental strains | French cypress essential oil Mic | Concentration for experiment |
| ATCC 25922 | 0.313％ | 1％ |
Example 3 fungicidal Effect of France cypress essential oil on ATCC25922 Strain after 30 minutes
1. Experimental materials:
(1) Test medium: the MH agar medium and the MacConkey agar medium (purchased from Guangdong Huancao Microscience Co., ltd.) which were sterilized by autoclaving were cooled to 40 ℃, 20mL of the MH agar medium and the MacConkey agar medium were put into a sterile petri dish using a pipette, and naturally dried for 30min to obtain the MH agar medium and the MacConkey agar medium.
Coli standard strain ATCC25922 (laboratory collection).
2. Preparation work before testing:
(1) Taking out the French cypress essential oil from the refrigerator, balancing to room temperature, and sucking part of the French cypress essential oil for later use;
(2) The E.coli standard strain ATCC25922 was cultured on MacconyKa medium to a suitable size.
3. Experiment for evaluating bactericidal effect of France cypress essential oil:
(1) Inoculating Escherichia coli ATCC25922, placing single colony in a centrifuge tube filled with 4mL MH broth, putting the centrifuge tube in a 37-degree shaking table, incubating for 4 hours at 180 revolutions, and taking out the centrifuge tube;
(2) Placing the centrifuge tube in a centrifuge, centrifuging for 8min at 5000 rpm, pouring out supernatant, adding equal volume of physiological saline for resuspension, and performing gradient dilution to obtain final bacterial load of 10 6 CFU/mL；
(3) Adding 1mL of bacterial liquid into a six-hole plate, adding 10 mu L of French cypress essential oil with the original concentration, and uniformly mixing to ensure that the final concentration of the essential oil is 1%;
(4) Setting contrast, blank contrast, and 1% of French cypress essential oil for 30min;
(5) And (3) sucking 100 mu L of bacterial liquid, adding the bacterial liquid into a 2ml centrifuge tube filled with 900 mu L of 0.85% physiological saline, carrying out gradient dilution, sucking 25 mu L of the diluted bacterial liquid, dripping the diluted bacterial liquid on an MH agar culture medium, incubating the bacterial liquid in a 37-degree incubator for 16-18h, counting, and carrying out statistical analysis after three biological repetitions of experimental results.
The results are shown in Table 3, the bacterial numbers of the blank control group and the bacterial number of the French cypress essential oil group which acts for 30min are not obviously distinguished; it is shown that 1% of the extract oil of Cupressus Sempervirens did not change the number of bacteria after 30min treatment with ATCC 25922. It can be seen that 1% of the French cypress essential oil alone had no significant bacteriostatic effect on ATCC25922 bacteria.
TABLE 3 fungicidal Effect of France cypress essential oil on ATCC25922 Strain
Example 4 evaluation of the killing effect of UV and UV potentiators on E.coli ATCC25922
1. Experimental materials:
(1) The ultraviolet lamp used for the test is PHILIPS TL-D brand; wattage: 18W; voltage: 220V; wavelength: (UVA) 300-469nm; tube diameter of the lamp tube: 25mm; length: 60cm.
(2) Test medium: the MH agar medium and the MacConkey agar medium (purchased from Guangdong Huancao Microscience Co., ltd.) which were sterilized by autoclaving were cooled to 40 ℃, 20mL of the MH agar medium and the MacConkey agar medium were put into a sterile petri dish using a pipette, and naturally dried for 30min to obtain the MH agar medium and the MacConkey agar medium.
Coli standard strain ATCC25922 (laboratory collection).
2. Preparation work before the test:
(1) Starting an ultraviolet lamp and continuously irradiating for 30 minutes for preheating;
(2) Taking out the French cypress essential oil from the refrigerator, balancing to room temperature, and sucking part of the French cypress essential oil for later use;
(3) The E.coli standard strain ATCC25922 was cultured on MacconyKa agar medium to a suitable size.
3. Ultraviolet synergist effect evaluation experiment:
(1) Inoculating Escherichia coli ATCC25922, placing a single colony in a centrifuge tube filled with 4mL MH broth, putting the centrifuge tube in a 37-degree shaking table, incubating at 180rpm for 4 hours, and taking out the centrifuge tube;
(2) Placing the centrifuge tube in a centrifuge, centrifuging for 8min at 5000 rpm, pouring out supernatant, adding equal volume of physiological saline for resuspension, and performing gradient dilution to obtain final bacterial load of 10 6 CFU/mL；
(3) Adding 1mL of bacterial liquid into a six-hole plate, adding 10 mu L of French cypress essential oil with the original concentration, and uniformly mixing;
(4) Setting contrast, blank contrast, treating with 1% essential oil for shading, placing into ultraviolet irradiation box, and irradiating with ultraviolet for 30min;
(5) After the ultraviolet irradiation is finished, 100 mu L of bacterial liquid is absorbed and added into a 2ml centrifuge tube filled with 900 mu L of 0.85% physiological saline for gradient dilution, 25 mu L of bacterial liquid is absorbed and dropped on an MH agar culture medium after dilution, the culture box with the temperature of 37 ℃ is incubated for 16-18h, counting is carried out, and the statistical analysis is carried out after the experimental result is repeated through three biology.
In this experiment, a growth control group, an ultraviolet control group, an essential oil control group, and an ultraviolet plus essential oil test group were set, and the detection was performed according to the method of example 1.
The results are shown in table 4, where the bacteria in the blank control group grow normally, indicating that escherichia coli ATCC25922 can grow normally under the experimental conditions; under the irradiation of an ultraviolet lamp for 30min, the Escherichia coli ATCC25922 is not obviously reduced compared with a blank control group, which shows that the inhibition effect of the ultraviolet lamp on the Escherichia coli ATCC25922 is not obvious; the same results also show that 1% of the french cypress essential oil has no significant inhibitory effect on escherichia coli ATCC 25922; the combined action result of 30min shows that the synergistic effect of the ultraviolet rays and 1% of the French cypress essential oil has obvious bactericidal effect on Escherichia coli ATCC 25922.
TABLE 4 Bactericidal Effect of UV and France cypress essential oil combination on ATCC25922 Strain
The embodiments of the present invention have been described in detail, but the present invention is not limited to the described embodiments. It will be apparent to those skilled in the art that various changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, and the scope of protection is still within the scope of the invention.
Claims (4)
1. A method for sterilizing by combining ultraviolet with France Cupressus Sempervirens essential oil is characterized in that the irradiation time of ultraviolet is 25-35min, the concentration of France Cupressus Sempervirens essential oil is 1%, and the ultraviolet intensity is 2.4-3.0mW/cm 2 。
2. The method of claim 1, wherein the UV irradiation is preceded by preheating for 20-40min.
3. The method for sterilizing by ultraviolet combination with an essential oil of oriental thuja francis according to claim 2, comprising the steps of:
(1) Mixing the bacterial liquid with 1% of French cypress essential oil, placing the mixture in a six-hole plate, and setting ultraviolet irradiation conditions: wavelength range of 300-460nm, power of 18W, distance from six-hole plate to ultraviolet lamp tube of 8cm, ultraviolet intensity of 2.4-3.0mW/cm 2 ；
(2) Preheating the ultraviolet box for 30min, and placing the six-hole plate in the ultraviolet box for irradiating for 30min.
4. The method for sterilizing by combining ultraviolet with French cypress essential oil according to claim 3, wherein the final concentration of the bacterial liquid in step (1) is 10 6 CFU/mL。
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