CN113896576B - Composition for fertilizer adduction bacteria and method for increasing viable bacteria number in microbial fertilizer - Google Patents
Composition for fertilizer adduction bacteria and method for increasing viable bacteria number in microbial fertilizer Download PDFInfo
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- CN113896576B CN113896576B CN202111244190.8A CN202111244190A CN113896576B CN 113896576 B CN113896576 B CN 113896576B CN 202111244190 A CN202111244190 A CN 202111244190A CN 113896576 B CN113896576 B CN 113896576B
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/10—Solid or semi-solid fertilisers, e.g. powders
- C05G5/12—Granules or flakes
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/30—Layered or coated, e.g. dust-preventing coatings
- C05G5/37—Layered or coated, e.g. dust-preventing coatings layered or coated with a polymer
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Abstract
The invention relates to a composition for fertilizer plus bacteria and a method for increasing the number of live bacteria in a microbial fertilizer. The composition comprises: polyacrylamide, xanthan gum and sodium carboxymethyl cellulose. Therefore, the components are mutually cooperated and matched, and the viable count and viable retention rate of the microbial fertilizer can be effectively improved. The composition is added into the microbial fertilizer, and after 3 months of storage, the effective viable count can be more than or equal to 30 hundred million/g, and the viable retention rate is more than or equal to 65%.
Description
Technical Field
The invention belongs to the technical field of fertilizers, and particularly relates to a composition for fertilizer bacteria and a method for increasing the number of viable bacteria in a microbial fertilizer.
Background
Beneficial microorganisms in the fertilizer have obvious effects on improving the utilization rate of the fertilizer, repairing and conditioning soil, promoting the growth of crops, reducing crop diseases, improving the yield of the crops and improving the quality of the crops. The microbial agent can be colonized in soil to form a dominant population, inhibit the growth and the propagation of other harmful microorganisms, and even generate antagonism on part of pathogenic microorganisms so as to reduce the chance of infecting the rhizosphere of crops; the compound fertilizer can generate metabolites beneficial to crops in the growth and reproduction processes, can stimulate the growth of the crops, and can enhance the disease resistance and stress resistance of the crops. By applying the fertilizer containing phosphate-solubilizing and potassium-solubilizing microorganisms, indissolvable phosphorus and potassium in soil can be decomposed, and the fertilizer is convenient for crops to absorb and utilize. The more beneficial microorganisms in the fertilizer, the more remarkable the gain effect.
Most of the prior fertilizer bacteria adding technologies in the market only use molasses liquid or directly add bacteria powder into the fertilizer to add bacteria agents, the effective viable count of the fertilizer is about 2-10 hundred million/g, and the following defects exist: the retention rate of viable bacteria is low, and the retention rate of initial viable bacteria is less than 30 percent; in the storage process, the retention rate of the viable bacteria is greatly reduced again; the death of a large number of microorganisms leads to high costs.
Therefore, there is a need for improved compositions for fertilizer plus bacteria.
Disclosure of Invention
The present invention aims to ameliorate at least one of the above technical problems to at least some extent.
In order to improve the technical problem, the present invention provides a composition for a fertilizer application bacterium, the composition comprising: polyacrylamide, xanthan gum, and sodium carboxymethylcellulose. The polyacrylamide and the sodium carboxymethylcellulose have binding property, the xanthan gum has a microbial inoculum protection function, and the components are mutually cooperated and matched, so that the viable count and the viable retention rate of the microbial fertilizer can be effectively improved, and the production cost is reduced. When the composition is added into a microbial fertilizer, the number of effective viable bacteria in a storage period of 3 months can be more than or equal to 30 hundred million/g, and the retention rate of the viable bacteria is more than or equal to 65%.
According to an embodiment of the invention, the microbial fertilizer comprises a fertilizer and an applied bacterium.
According to an embodiment of the invention, the fertilizer is an organic fertilizer.
According to the embodiment of the invention, the organic fertilizer is a granular organic fertilizer, and the organic content is 45%.
According to an embodiment of the invention, the addicting bacterium is bacillus subtilis.
According to the embodiment of the invention, the effective viable count of the external bacteria is 2000 hundred million/g.
According to the embodiment of the invention, based on the total mass of the composition, the content of the polyacrylamide is 30-60 parts by weight, the content of the xanthan gum is 10-30 parts by weight, and the content of the sodium carboxymethyl cellulose is 5-30 parts by weight. Therefore, the viable count and viable bacteria retention rate of the microbial fertilizer can be further improved.
According to an embodiment of the invention, the composition further comprises: at least one of hydroxyethyl methylcellulose, protein powder, potassium fulvate, polyglutamic acid, trehalose, molasses powder, hydroxypropyl methylcellulose and polyaspartic acid. Therefore, the viable count and viable retention rate of the microbial fertilizer can be further improved, and the production cost is further reduced.
According to the embodiment of the invention, based on the total mass of the composition, the content of hydroxyethyl methyl cellulose is 0-50 parts by weight, the content of albumen powder is 0-30 parts by weight, the content of potassium fulvate is 0-5 parts by weight, the content of polyglutamic acid is 0-0.5 parts by weight, the content of trehalose is 0-0.5 parts by weight, the content of molasses powder is 0-0.5 parts by weight, the content of hydroxypropyl methyl cellulose is 0-0.5 parts by weight, and the content of polyaspartic acid is 0-0.5 parts by weight. Therefore, the viable count and viable bacteria retention rate of the microbial fertilizer can be further improved.
The invention also provides a method for increasing the number of viable bacteria in the microbial fertilizer, which comprises the following steps: formulating the composition as hereinbefore described as an adjuvant solution; and mixing the aid solution with a fertilizer and an additional bacterium. It is to be noted that this method has all the features and advantages of the composition for fertilizer addition bacteria described hereinbefore and will not be described in detail here. In general, the method can effectively improve the viable count and viable retention rate of the microbial fertilizer.
According to an embodiment of the invention, the mixing comprises: spraying the aid solution on the surface of the fertilizer; mixing the fertilizer sprayed with the bacteria attached to the surface of the fertilizer; and spraying the auxiliary agent solution on the surface of the fertilizer attached with the external bacteria. The auxiliary agent can be attached to the surface of the fertilizer by the first spraying, so that the fertilizer and the bacterial powder are isolated, the damage of the fertilizer and substances in the fertilizer to the bacterial powder is reduced, and the auxiliary agent on the outer surface of the fertilizer can be bonded with the bacterial powder for the first time; the secondary spraying can wrap the bonded bacterial powder through the sprayed auxiliary agent solution, so that the bacterial powder is more firmly adhered to the fertilizer, and the bacterial powder which cannot be firmly adhered in the previous step is secondarily adhered to the fertilizer, so that the retention rate of the bacterial powder on the fertilizer is improved.
According to an embodiment of the invention, the mass fraction of the adjuvant solution is 0.1-5%. Therefore, the auxiliary agent can be uniformly coated on the surface of the fertilizer particles and firmly bonded on the surface of the fertilizer particles.
According to an embodiment of the invention, the mixing of the adjuvant solution with the fertilizer and the applied bacteria is carried out in a coating drum; and a spray head is arranged in the wrapping roller and is used for spraying the auxiliary agent solution.
According to an embodiment of the invention, the distance between the spray head and the fertilizer surface is not more than 500 mm. Evaporation of the solvent from the adjuvant solution on reaching the fertilizer surface can be reduced.
According to the embodiment of the invention, the distance between the additional bacteria outlet and the surface of the fertilizer is not more than 200mm, so that the flying loss of the additional bacteria in the roller can be effectively reduced.
Detailed Description
Embodiments of the present application are described in detail below. The following description of the embodiments is merely exemplary in nature and is in no way intended to limit the present disclosure. The examples, where specific techniques or conditions are not indicated, are to be construed according to the techniques or conditions described in the literature in the art or according to the product specifications. The reagents used are not indicated by manufacturers, and are all conventional products available on the market.
Most of the prior fertilizer bacterium adding technologies in the market only use molasses liquid or directly add bacterium powder into the fertilizer to add bacterium agents, the effective viable count of the fertilizer is about 2-10 hundred million/g, and the following main defects exist: the retention rate of viable bacteria is low, and the retention rate of initial viable bacteria is less than 30 percent; after the beneficial microorganisms are combined with the fertilizer, the beneficial microorganisms die continuously in the storage process, so that the retention rate of viable bacteria in the storage period is greatly reduced again; the death of a large number of microorganisms causes the cost of bacteria addition to be high.
In order to improve the technical problem, the present invention provides a composition for a fertilizer application bacterium, the composition comprising: polyacrylamide, xanthan gum and sodium carboxymethyl cellulose. The composition can enable the bacterial powder to be attached or adhered to the surface of the fertilizer particles, and meanwhile, the composition can provide a strong protection function, so that the viable count and viable retention rate of the microbial fertilizer are effectively improved. When the composition is added into a microbial fertilizer, the viable bacteria retention rate is more than or equal to 65 percent in a storage period of 3 months, and the effective viable bacteria number is more than or equal to 30 hundred million/g.
Specifically, the calculation formula of the viable bacteria retention rate is as follows:
the viable bacteria retention rate is the effective viable bacteria number in the fertilizer during detection divided by the actual effective viable bacteria number added into the fertilizer multiplied by 100 percent
According to the embodiment of the present invention, the polyacrylamide is contained in an amount of 30 to 60 parts by weight, based on the total mass of the composition. If the content of polyacrylamide is too low, the binding effect of the composition is low, and the binding effect on the fertilizer and the external bacteria is poor. If the polyacrylamide content is too high, this results in a too high viscosity of the composition, which is detrimental to the delivery of the adjuvant solution formed from the composition. When the content of the polyacrylamide is 30-60 parts by weight, the bonding effect of the composition is proper, the fertilizer and the external bacteria have better bonding effect, and the delivery of the auxiliary agent solution formed by the composition is convenient. Illustratively, the parts by weight of the polyacrylamide can be 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60.
According to the examples of the present invention, the xanthan gum is contained in an amount of 10 to 30 parts by weight, based on the total mass of the composition. If the content of the xanthan gum is too low, the protective effect of the microbial inoculum is poor. If the xanthan gum is present in too high a level, the corresponding other components are present in a lower level, which may result in a less cohesive composition. When the content of the xanthan gum is 10-30 parts by weight, the composition has a good microbial inoculum protection effect and a good bonding effect. For example, the xanthan gum may be present in 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30 parts by weight.
According to the embodiment of the present invention, the content of the sodium carboxymethylcellulose is 5 to 30 parts by weight based on the total mass of the composition. If the content of the sodium carboxymethylcellulose is too low, the bonding effect is poor. If the content of the sodium carboxymethylcellulose is too high, the content of the xanthan gum is relatively low, so that the microbial inoculum protection effect of the composition is poor, and the production cost of the composition is increased. When the content of the sodium carboxymethylcellulose is 5-30 parts by weight, the composition has a good bonding effect, a good microbial inoculum protection effect and a low production cost. Illustratively, the parts by weight of sodium carboxymethylcellulose can be 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30.
According to an embodiment of the invention, the microbial fertilizer comprises a fertilizer and an applied bacterium. In some embodiments of the invention, the fertilizer is an organic fertilizer, which is in the form of granules.
Wherein the organic fertilizer is a granular organic fertilizer with the organic matter content of 45 percent.
The additional bacteria is Bacillus subtilis.
According to the embodiment of the invention, the effective viable count of the external bacteria is 2000 hundred million/g.
According to an embodiment of the invention, the composition further comprises: at least one of hydroxyethyl methylcellulose, protein powder, potassium fulvate, polyglutamic acid, trehalose, molasses powder, hydroxypropyl methylcellulose and polyaspartic acid. Therefore, the viable count and viable retention rate of the microbial fertilizer can be further improved, and the production cost is further reduced.
According to an embodiment of the present invention, the content of the hydroxyethyl methylcellulose may be 0 to 50 parts by weight, based on the total mass of the composition. If the content of hydroxyethyl methylcellulose is too high, the production cost of the composition increases. When the content of the hydroxyethyl methylcellulose is 0 to 50 parts by weight, there is an advantage of low production cost. Illustratively, the weight fraction of hydroxyethyl methylcellulose can be 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50.
According to the embodiment of the present invention, the content of the protein powder may be 0 to 30 parts by weight based on the total mass of the composition. If the protein powder content is too high, the production cost of the composition is increased. When the content of the protein powder is 0-30 parts by weight, the microbial inoculum has a better protective function and has the advantage of low production cost. For example, the protein powder may be 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30 parts by weight.
According to the embodiment of the invention, the content of the potassium fulvate is 0-5 parts by weight based on the total mass of the composition, and if the content of the potassium fulvate is too high, the water absorption of the surface of the fertilizer is increased, and the storage time of the microbial fertilizer is shortened. When the content of the potassium fulvate is 0-5 parts by weight, the composition has a good microbial inoculum protection function, and meanwhile, the storage period of the fertilizer is not shortened. Illustratively, the parts by weight of the potassium fulvate can be 0, 1, 2, 3, 4, 5.
According to the embodiment of the present invention, the content of the polyglutamic acid is 0 to 0.5 parts by weight, based on the total mass of the composition. If the content of polyglutamic acid is too high, the production cost of the composition increases. When the content of polyglutamic acid is 0-0.5 parts by weight, it has an advantage of low production cost. Illustratively, the weight fraction of the polyglutamic acid may be 0, 0.1, 0.2, 0.3, 0.4, 0.5.
According to the embodiment of the present invention, trehalose is contained in an amount of 0 to 0.5 parts by weight, based on the total mass of the composition. If the content of trehalose is too high, the production cost of the composition increases. When the content of trehalose is 0-0.5 parts by weight, the production cost is low. Illustratively, the trehalose may be in parts by weight of 0, 0.1, 0.2, 0.3, 0.4, 0.5.
According to the embodiment of the present invention, the content of the molasses powder is 0-0.5 weight part based on the total mass of the composition. For example, if the fructose honey powder content is too high, the viscosity of the adjuvant solution formed from the composition may be reduced. When the content of the molasses powder is 0-0.5 weight part, the composition has proper viscosity, and can enable the fertilizer and the bacterial powder to have better bonding effect. For example, the honey powder may be 0, 0.1, 0.2, 0.3, 0.4, 0.5 by weight.
According to the embodiment of the present invention, the content of the hydroxypropylmethylcellulose is 0 to 0.5 parts by weight based on the total mass of the composition. If the content of hydroxypropylmethylcellulose is too high, the viscosity of the adjuvant solution formed from the composition is lowered. When the content of the hydroxypropyl methyl cellulose is 0 to 0.5 weight part, the auxiliary agent solution formed by the composition has proper flowing property and viscosity, is convenient to transport, and can also enable the fertilizer and the bacterial powder to have better bonding effect. Illustratively, the weight parts of the hydroxypropyl methylcellulose can be 0, 0.1, 0.2, 0.3, 0.4, 0.5.
According to the embodiment of the present invention, the content of polyaspartic acid is 0 to 0.5 parts by weight based on the total mass of the composition. If the content of polyaspartic acid is too high, the viscosity of the adjuvant solution formed from the composition may be lowered. When the content of the polyaspartic acid is 0 to 0.5 part by weight, the composition can have a good microbial inoculum protection effect, and an auxiliary agent solution formed by the composition can have good viscosity. Illustratively, the polyaspartic acid may be present in an amount of 0, 0.1, 0.2, 0.3, 0.4, 0.5 parts by weight.
The invention also provides a method for increasing the number of viable bacteria in the microbial fertilizer, which comprises the following steps:
s100, preparing a composition for the fertilizer plus bacteria into an auxiliary agent solution;
the composition for the fertilizer plus bacteria is mixed with a solvent to obtain an auxiliary agent solution. According to an embodiment of the invention, the solvent used for the dissolution of the composition is water.
According to an embodiment of the invention, the mass fraction of the adjuvant solution is 0.1-5%. If the mass fraction of the additive solution is too low, the adhesive property of the additive solution is poor. If the mass fraction of the auxiliary agent solution is too high, the viscosity of the auxiliary agent solution is too high, so that the conveying difficulty is increased, and the uniformity of the auxiliary agent solution coated on the surface of the fertilizer is poor. When the mass fraction of the auxiliary agent solution is 0.1-5%, the viscosity of the auxiliary agent solution is appropriate, the auxiliary agent solution can be uniformly coated on the surface of the fertilizer, and meanwhile, the auxiliary agent solution can be easily conveyed.
In some embodiments of the invention, the mass fraction of the adjuvant solution may be 0.1%, 0.5%, 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5%, 5%.
S200, mixing the aid solution with the fertilizer and the additional bacteria.
According to an embodiment of the invention, the mixing of the adjuvant solution with the fertilizer and the applied bacteria is carried out in a coating drum. A spray head is arranged in the wrapping roller and used for spraying the auxiliary agent solution.
According to the embodiment of the invention, the spray head is arranged at the front end of the inner part of the wrapping roller. The number of the nozzles is not limited in the invention, and the nozzles can be set by a person skilled in the art according to the use requirement.
According to an embodiment of the invention, the mixing comprises:
s210, spraying the aid solution on the surface of the fertilizer;
according to an embodiment of the invention, the adjuvant solution is pumped through a pump and sprayed through a spray head.
The distance between the spray head and the surface of the fertilizer granules is not more than 500 mm. If the distance is too large, the spray droplets fly in the drum, resulting in increased losses. When the distance between the spray head and the surface of the fertilizer granules is not more than 500mm, the additive solution can be uniformly sprayed on the surface of the fertilizer, and the loss of the additive solution in the spraying process can be reduced.
S220, mixing the sprayed fertilizer with external bacteria, and attaching the external bacteria to the surface of the fertilizer;
the invention is not limited to specific parameters of mixing, and the skilled person can adjust the mixing according to the use requirement.
And S230, spraying an auxiliary agent solution on the surface of the fertilizer with the attached additional bacteria.
After the mixing, i.e., after the completion of step S230, the organic fertilizer is discharged through the outlet of the wrapping drum.
The distance between the outlet and the surface of the organic fertilizer is not more than 200 mm. If the distance is too large, the bacterial powder can fly in the roller due to the light specific gravity of the bacterial powder, and the loss is increased. When the distance between the outlet and the surface of the fertilizer is not more than 200mm, the flying loss of the bacterial powder in the roller can be effectively reduced.
The invention will now be described with reference to specific examples, which are intended to be illustrative only and not to be limiting in any way.
In all the following examples, the mass ratio of the auxiliary agent solution to the granular organic fertilizer is 6: 1000, parts by weight; the method for detecting the effective viable count of the added bacteria product is NYT 798-.
In examples 1 to 6 and comparative examples 1 to 4, the same amount of bacteria, the same amount of fertilizer and the same amount of adjuvant were used.
The calculated effective viable count of the fertilizer refers to a calculated value of the effective viable count actually added into the organic fertilizer, and the calculated effective viable count of the fertilizer is the number of bacteria added into the fertilizer divided by the number of bacteria added into the fertilizer and the total mass of the fertilizer after addition of the bacteria and the additives.
The initial retention rate is: and mixing the composition with the bacterial powder and the organic fertilizer particles to obtain a product, sampling from the product, checking the number of viable bacteria, and calculating the viable bacteria retention rate according to a calculation formula of the viable bacteria retention rate.
Specifically, the calculation formula of the viable bacteria retention rate is as follows:
the retention rate of viable bacteria is equal to the effective viable bacteria number in the fertilizer at the time of detection ÷ the actual effective viable bacteria number added into the fertilizer multiplied by 100%
Example 1
The composition for the fertilizer addition bacteria comprises polyacrylamide, xanthan gum, sodium carboxymethylcellulose, hydroxyethyl methylcellulose and potassium fulvate, and the mass ratio of the substances is 60%: 10%: 29%: 0.5%: 0.5 percent.
The method for increasing the number of viable bacteria in the microbial fertilizer comprises the following steps: weighing the components according to the content, mixing, adding a solvent, and preparing into an auxiliary agent solution with the mass concentration of 5.0%. Pressurizing by a pump, uniformly spraying a layer of auxiliary agent solution on the surfaces of the organic fertilizer particles by a spray head arranged at the front end in the wrapping roller, adding required amount of bacterial powder into the roller, uniformly spraying a layer of auxiliary agent solution on the surfaces of the organic fertilizer particles adhered with the bacterial powder, and taking the obtained product out of the wrapping roller to obtain a finished product which is sent to a subsequent working section.
The effective viable count of the fertilizer is calculated to be 46.2 hundred million/g.
And (3) detection results: the initial viable bacteria retention rate is 77.3 percent, the effective viable bacteria number in 3 months of storage period is 34.5 hundred million/g, and the viable bacteria retention rate is 74.7 percent.
Example 2
The composition for the fertilizer addictives consists of polyacrylamide, xanthan gum, sodium carboxymethylcellulose, hydroxyethyl methylcellulose, protein powder and trehalose, wherein the mass ratio of the substances is 45.0%: 18.0%: 15.0%: 20.5%: 1.0%: 0.5 percent.
The method for increasing the number of viable bacteria in the microbial fertilizer comprises the following steps: weighing the components according to the content, mixing, adding a solvent, and preparing into an auxiliary agent solution with the mass concentration of 3.0%. Pressurizing by a pump, uniformly spraying a layer of auxiliary agent solution on the surfaces of the organic fertilizer particles by a spray head arranged at the front end in the wrapping roller, adding required amount of bacterial powder into the roller, uniformly spraying a layer of auxiliary agent solution on the surfaces of the organic fertilizer particles adhered with the bacterial powder, and taking the obtained product out of the wrapping roller to obtain a finished product which is sent to a subsequent working section.
The effective viable count of the fertilizer is calculated to be 46.2 hundred million/g.
And (3) detection results: the initial viable bacteria retention rate is 86.0 percent, the effective viable bacteria number in 3 months of storage period is 37.6 hundred million/g, and the viable bacteria retention rate is 81.4 percent.
Example 3
The composition for the fertilizer external bacteria comprises polyacrylamide, xanthan gum, sodium carboxymethylcellulose, hydroxyethyl methylcellulose, protein powder, polyglutamic acid, molasses, hydroxypropyl methylcellulose and polyaspartic acid, wherein the mass ratio of the substances is 30.0%: 30.0%: 5.0%: 15.5%: 18.5%: 0.3%: 0.2%: 0.4%: 0.1 percent.
The method for increasing the number of viable bacteria in the microbial fertilizer comprises the following steps: weighing the components according to the content, mixing, adding a solvent, and preparing into an auxiliary agent solution with the mass concentration of 1.0%. Pressurizing by a pump, uniformly spraying a layer of auxiliary agent solution on the surfaces of the organic fertilizer particles by a spray head arranged at the front end in the wrapping roller, adding required amount of bacterial powder into the roller, uniformly spraying a layer of auxiliary agent solution on the surfaces of the organic fertilizer particles adhered with the bacterial powder, and taking the obtained product out of the wrapping roller to obtain a finished product which is sent to a subsequent working section.
The effective viable count of the fertilizer is calculated to be 46.2 hundred million/g.
And (3) detection results: the initial viable bacteria retention rate is 75.6 percent, the effective viable bacteria number in 3-month storage period is 32.8 hundred million/g, and the viable bacteria retention rate is 71.0 percent.
Example 4
The composition for the fertilizer addictives consists of polyacrylamide, xanthan gum, sodium carboxymethylcellulose, hydroxyethyl methylcellulose, potassium fulvate, polyglutamic acid and polyaspartic acid, and the mass ratio of the substances is 40.0%: 5.0%: 35.0%: 3.5%: 15.0%: 1.0%: 0.5 percent.
The method for increasing the number of viable bacteria in the microbial fertilizer comprises the following steps: weighing the materials according to the content, mixing, adding a solvent, and preparing into an auxiliary agent solution with the mass concentration of 0.1%. Pressurizing by a pump, uniformly spraying a layer of auxiliary agent solution on the surfaces of the organic fertilizer particles by a spray head arranged at the front end in the wrapping roller, adding required amount of bacterial powder into the roller, uniformly spraying a layer of auxiliary agent solution on the surfaces of the organic fertilizer particles adhered with the bacterial powder, and taking the obtained product out of the wrapping roller to obtain a finished product which is sent to a subsequent working section.
The effective viable count of the fertilizer is calculated to be 46.2 hundred million/g.
And (3) detection results: the initial viable bacteria retention rate is 92.8 percent, the effective viable bacteria number in 3 months of storage period is 32.8 hundred million/g, and the viable bacteria retention rate is 71.1 percent.
Example 5
The composition for the fertilizer addition bacteria consists of polyacrylamide, xanthan gum and sodium carboxymethyl cellulose, and the mass ratio of the substances is 40.0%: 30.0%: 30.0 percent.
The method for increasing the number of viable bacteria in the microbial fertilizer comprises the following steps: weighing the components according to the content, mixing, adding a solvent, and preparing into an auxiliary agent solution with the mass concentration of 3.0%. Pressurizing by a pump, uniformly spraying a layer of auxiliary agent solution on the surfaces of the organic fertilizer particles by a spray head arranged at the front end in the wrapping roller, adding required amount of bacterial powder into the roller, uniformly spraying a layer of auxiliary agent solution on the surfaces of the organic fertilizer particles adhered with the bacterial powder, and taking the obtained product out of the wrapping roller to obtain a finished product which is sent to a subsequent working section.
The effective viable count of the fertilizer is calculated to be 46.2 hundred million/g.
And (3) detection results: the initial viable bacteria retention rate is 70.3 percent, the effective viable bacteria number in 3 months of storage period is 32.0 hundred million/g, and the viable bacteria retention rate is 69.3 percent.
Example 6
The composition for the fertilizer addition bacteria comprises polyacrylamide, xanthan gum and sodium carboxymethylcellulose, and the mass ratio of the substances is 30.0%: 40.0%: 30.0 percent.
The method for increasing the number of viable bacteria in the microbial fertilizer comprises the following steps: weighing the components according to the content, mixing, adding a solvent, and preparing into an auxiliary agent solution with the mass concentration of 3.0%. Pressurizing by a pump, uniformly spraying a layer of auxiliary agent solution on the surfaces of the organic fertilizer particles by a spray head arranged at the front end in the wrapping roller, adding required amount of bacterial powder into the roller, uniformly spraying a layer of auxiliary agent solution on the surfaces of the organic fertilizer particles adhered with the bacterial powder, and taking the obtained product out of the wrapping roller to obtain a finished product which is sent to a subsequent working section.
The effective viable count of the fertilizer is calculated to be 46.2 hundred million/g.
And (3) detection results: the initial viable bacteria retention rate is 65.6 percent, the effective viable bacteria number in 3 months of storage period is 31.7 hundred million/g, and the viable bacteria retention rate is 68.6 percent.
Comparative example 1
The composition for the fertilizer addition bacteria consists of polyacrylamide and xanthan gum, and the mass ratio of the substances is 40.0%: 60.0 percent.
The method for increasing the number of viable bacteria in the microbial fertilizer comprises the following steps: weighing the components according to the content, mixing, adding a solvent, and preparing into an auxiliary agent solution with the mass concentration of 3.0%. Pressurizing by a pump, uniformly spraying a layer of auxiliary agent solution on the surfaces of the organic fertilizer particles by a spray head arranged at the front end in the wrapping roller, adding required amount of bacterial powder into the roller, uniformly spraying a layer of auxiliary agent solution on the surfaces of the organic fertilizer particles adhered with the bacterial powder, and taking the obtained product out of the wrapping roller to obtain a finished product which is sent to a subsequent working section.
The effective viable count of the fertilizer is calculated to be 46.2 hundred million/g.
And (3) detection results: the initial viable bacteria retention rate is 68.1 percent, the effective viable bacteria number in 3 months of storage period is 16.9 hundred million/g, and the viable bacteria retention rate is 36.6 percent.
Comparative example 2
The composition for the fertilizer addition bacteria consists of polyacrylamide and sodium carboxymethylcellulose, and the mass ratio of the substances is 70.0%: 30.0 percent.
The method for increasing the number of viable bacteria in the microbial fertilizer comprises the following steps: weighing the components according to the content, mixing, adding a solvent, and preparing into an auxiliary agent solution with the mass concentration of 3.0%. Pressurizing by a pump through a spray nozzle arranged at the front end in the wrapping roller, uniformly spraying a layer of auxiliary agent solution on the surfaces of the organic fertilizer particles, adding required amount of fungus powder into the roller, uniformly spraying a layer of auxiliary agent solution on the surfaces of the organic fertilizer particles adhered with the fungus powder, and taking the obtained product out of the wrapping roller to obtain a finished product which is sent to a subsequent working section.
The effective viable count of the fertilizer is calculated to be 46.2 hundred million/g.
And (3) detection results: the initial viable bacteria retention rate is 75.6 percent, the effective viable bacteria number in 3 months of storage period is 18.3 hundred million/g, and the viable bacteria retention rate is 39.6 percent.
Comparative example 3
The composition for the fertilizer addition bacteria consists of xanthan gum and sodium carboxymethyl cellulose, and the mass ratio of the components is 70.0%: 30.0 percent.
The method for increasing the number of viable bacteria in the microbial fertilizer comprises the following steps: weighing the components according to the content, mixing, adding a solvent, and preparing into an auxiliary agent solution with the mass concentration of 3.0%. Pressurizing by a pump, uniformly spraying a layer of auxiliary agent solution on the surfaces of the organic fertilizer particles by a spray head arranged at the front end in the wrapping roller, adding required amount of bacterial powder into the roller, uniformly spraying a layer of auxiliary agent solution on the surfaces of the organic fertilizer particles adhered with the bacterial powder, and taking the obtained product out of the wrapping roller to obtain a finished product which is sent to a subsequent working section.
The effective viable count of the fertilizer is calculated to be 46.2 hundred million/g.
And (3) detection results: the initial viable bacteria retention rate is 44.9 percent, the effective viable bacteria number in 3 months of storage period is 16.0 hundred million/g, and the viable bacteria retention rate is 34.6 percent.
Comparative example 4
By utilizing the prior art, the same amount of molasses liquid is used for replacing the composition solution of the invention to carry out the bacteria-adding test.
And spraying molasses liquid on the surfaces of organic fertilizer particles by using a pump by adopting an industrial common adding method, adding bacterial powder which is equal to that in the previous embodiment into the roller, and wrapping the obtained product with the roller to obtain a finished product which is sent to a subsequent working section.
The effective viable count of the fertilizer is calculated to be 46.2 hundred million/g.
And (3) detection results: the initial viable bacteria retention rate is 34.2 percent, the effective viable bacteria number in 3 months of storage period is 10.8 hundred million/g, and the viable bacteria retention rate is 23.4 percent.
In the description herein, references to the description of the term "one embodiment," "some embodiments," "an example," "a specific example," or "some examples," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above are not necessarily intended to refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, various embodiments or examples and features of different embodiments or examples described in this specification can be combined and combined by one skilled in the art without contradiction.
Although embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention, and that variations, modifications, substitutions and alterations can be made to the above embodiments by those of ordinary skill in the art within the scope of the present invention.
Claims (9)
1. The application of the bonding composition for the fertilizer plus bacteria in improving the number of the viable bacteria in the microbial fertilizer is characterized in that,
preparing the bonding composition into an auxiliary agent solution;
spraying the aid solution on the surface of the fertilizer;
mixing the fertilizer subjected to spraying with an additional bacterium, wherein the additional bacterium is attached to the surface of the fertilizer;
spraying the auxiliary agent solution on the surface of the fertilizer attached with the external bacteria;
wherein the bonding composition comprises: polyacrylamide, xanthan gum, and sodium carboxymethylcellulose;
based on the total mass of the bonding composition, the content of the polyacrylamide is 30-60 parts by weight, the content of the xanthan gum is 10-30 parts by weight, and the content of the sodium carboxymethylcellulose is 5-30 parts by weight;
the additional bacteria are bacillus subtilis.
2. Use according to claim 1, characterized in that the fertilizer is a granular organic fertilizer.
3. The use of claim 1, wherein the bonding composition further comprises: at least one of hydroxyethyl methylcellulose, protein powder, potassium fulvate, polyglutamic acid, trehalose, molasses powder, hydroxypropyl methylcellulose and polyaspartic acid.
4. The use according to claim 3, wherein the hydroxyethyl methylcellulose is present in an amount of 0 to 50 parts by weight, the protein powder is present in an amount of 0 to 30 parts by weight, the potassium fulvate is present in an amount of 0 to 5 parts by weight, the polyglutamic acid is present in an amount of 0 to 0.5 parts by weight, the trehalose is present in an amount of 0 to 0.5 parts by weight, the molasses is present in an amount of 0 to 0.5 parts by weight, the hydroxypropyl methylcellulose is present in an amount of 0 to 0.5 parts by weight, and the polyaspartic acid is present in an amount of 0 to 0.5 parts by weight, based on the total weight of the cementitious composition.
5. A method for increasing the number of viable bacteria in a microbial fertilizer, the method comprising: preparing the bonding composition into an auxiliary agent solution;
spraying the aid solution on the surface of the fertilizer;
mixing the fertilizer subjected to spraying with an additional bacterium, wherein the additional bacterium is attached to the surface of the fertilizer;
spraying the auxiliary agent solution on the surface of the fertilizer attached with the external bacteria;
the bonding composition includes: polyacrylamide, xanthan gum and sodium carboxymethylcellulose;
based on the total mass of the bonding composition, the content of the polyacrylamide is 30-60 parts by weight, the content of the xanthan gum is 10-30 parts by weight, and the content of the sodium carboxymethylcellulose is 5-30 parts by weight;
the additional bacteria are bacillus subtilis.
6. The method according to claim 5, characterized in that the mass fraction of the adjuvant solution is 0.1-5%.
7. The method according to claim 5, characterized in that the mixing of the adjuvant solution with the fertilizer and the applied bacteria is carried out in a coating drum;
and a spray head is arranged in the wrapping roller and is used for spraying the auxiliary agent solution.
8. The method of claim 7, wherein the distance from the spray head to the fertilizer surface is no greater than 500 mm.
9. The method according to claim 7, characterized in that after the adjuvant solution is mixed with the fertilizer and the applied bacteria, the fertilizer is discharged through the outlet of the wrapping drum;
the distance between the additional bacteria outlet and the surface of the fertilizer is not more than 200 mm.
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| CN111285723A (en) * | 2020-02-26 | 2020-06-16 | 江苏思威博生物科技有限公司 | Composite auxiliary agent capable of improving survival rate of strains in bio-organic fertilizer and use method thereof |
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| CN111285723A (en) * | 2020-02-26 | 2020-06-16 | 江苏思威博生物科技有限公司 | Composite auxiliary agent capable of improving survival rate of strains in bio-organic fertilizer and use method thereof |
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