CN113876614B - Glycyrrhizin and tremella aurantialba polysaccharide degradation product compound composition and application thereof - Google Patents
Glycyrrhizin and tremella aurantialba polysaccharide degradation product compound composition and application thereof Download PDFInfo
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- CN113876614B CN113876614B CN202111180573.3A CN202111180573A CN113876614B CN 113876614 B CN113876614 B CN 113876614B CN 202111180573 A CN202111180573 A CN 202111180573A CN 113876614 B CN113876614 B CN 113876614B
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/63—Steroids; Derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
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- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The invention discloses a compound composition of Glycyrrhizic Anhydride (GAMS) and auricularia auricular polysaccharide degradation products (TAP), wherein the active ingredients of the compound composition are composed of the glycyrrhizic anhydride and the auricularia auricular polysaccharide degradation products; the weight ratio of the glycyrrhizic anhydride to the auricularia auricula polysaccharide degradation product is 1:1-1:10. It was found that GAMS and TAP were administered in combination, especially when the weight ratio was 1:1-1:10, the product has good moisturizing synergy; the inventor determines the in vitro moisturizing activity, the protection effect of a sample on dry and damaged HaCaT cells, the influence of the sample on the AQP3 content in the dry and damaged HaCaT cells, and adds the moisturizing composition into a cosmetic variety, and determines the transepidermal water loss rate at different times, so that the moisturizing composition has the effects of improving the self anti-drying capacity of the cells on the molecular level and the cosmetics have stronger moisturizing effect.
Description
Technical Field
The invention relates to the technical field of cosmetics, in particular to a compound composition of glycyrrhizic anhydride and auricularia auricular polysaccharide degradation products and application thereof.
Background
Skin moisturization is the most basic need for skin care. The horny layer of human skin mainly consists of coreless horny cells, keratin filaments, amino acid compounds and natural moisturizing factors, and has the functions of moisture retention, barrier and the like. The moisture source in the skin cuticle is mainly sweat secreted by sweat glands, and 20% of water is tightly combined with natural moisturizing factors and skin lipids, so that the formed sebum film formed by emulsifying sweat and water and the natural moisturizing factors become two most important factors for skin moisturizing. The moisturizing effect of cosmetics and raw materials can be evaluated by using indexes such as Natural Moisturizing Factors (NMF), aquaporins (AQPs), claudins, hyaluronic Acid (HA) and the like in skin keratinocytes cultured in vitro.
Aquaporins (AQPs) are proteins located on the cell membrane that significantly increase the permeability of the cell membrane to water molecules. Because the epidermis is avascular, the water in the epidermis diffuses from the dermis, and thus the AQPs between the epidermis cells constitute the major pathways for intercellular water transport. To date, 13 AQPs have been found, of which AQP3 is the most abundantly expressed aquaporin subtype in human skin, AQP3 being permeable to glycerol and the like in addition to water molecules. At present, western Blot hybridization, immunohistochemical staining method semi-quantitative detection and commercial kit ELISA method are mainly adopted to quantitatively detect the change of the AQP3 content, and the method can be used as one of evaluation indexes of the effective components of the moisturizing effect of cosmetics.
There has been a great deal of research at present showing that glycyrrhizin (Glycyrrhizic Acid Monoammonium Salt n-hydrochloride, GAMS) has the efficacy of antivirus, protecting liver, reducing transaminase, immunomodulation, protecting cell membrane, reducing inflammatory reaction, etc. (Chai Yigong. A-2b interferon combined with compound glycyrrhizin for clinical observation of chronic hepatitis b [ J. Shandong medicine,2011, 33 (1): 108-109.). Because the glycyrrhizic acid preparation has similar pharmacological effects to that of glucocorticoid medicines in anti-inflammatory aspect and no obvious side effect, the glycyrrhizic acid preparation is mainly used for treating bronchitis and acute and chronic hepatitis caused by various reasons clinically, and has the effect of resisting HIV. Glycyrrhizic acid drugs are getting more and more attention, and some other effects thereof are slowly known. Guo et al found that AQP 3expression was increased in HaCat cells treated with appropriate concentrations of Compound Glycyrrhizin (CG), presumably providing a skin barrier repairing effect in addition to anti-inflammatory effects of CG (Guo Bo, song Weimin, hu Lining. Effect of compound glycyrrhizin on the aquaporin3expression in HaCaT cell cultured in vitro [ J ]. Chinese Journal of Dermatovenereology of Integrated Traditional & Western Medicine,2011, 10 (4): 219-220.).
The tremella aurantialba (Tremella aurantialba, TA) is a rare and precious edible fungus, and Chinese medicinal fungi record that the tremella aurantialba has Wen Zhongdai cold property and sweet taste, can resolve phlegm and relieve cough, is mainly used for treating diseases such as lung heat, excessive phlegm, asthma and the like, and has the effects of lubricating skin and the like due to fine and smooth tremella aurantialba colloid, white and lubrication and delicate smell. The tremella aurantialba polysaccharide has a relatively large molecular weight and is difficult to absorb, the absorption rate of a human body to TAP is improved after the molecular weight of the tremella aurantialba polysaccharide is reduced (Tremella aurantialba polysaccharide, TAP), and researches on the moisturizing effect of the polysaccharide acid degradation product after the tremella aurantialba water extraction acid degradation are also reported (Zhicai Zhang, yin Li; kechang Zhang, application of Statistical Analysis for the Optimization of Mycelia and Polysaccharide Production by Tremella aurantialba, food Technology and Biotechnology, volume 45,Issue 1.2007.PP 45-50).
However, the invention discovers that no one researches the synergistic effect of the moisturizing effect of the degradation products of the glycyrrhizic anhydride and the auricularia auricula polysaccharide in the research process.
Disclosure of Invention
In order to overcome the defects and shortcomings in the prior art, the primary purpose of the invention is to provide a compound composition of glycyrrhizic anhydride and auricularia auricula polysaccharide degradation products; the active ingredients of the compound composition consist of glycyrrhizic anhydride and auricularia auricula polysaccharide degradation products.
The invention also aims to provide an application of the compound composition in preparing a moisturizing cosmetic; pharmacological tests prove that the compound composition has the synergistic moisturizing effect.
A compound composition of glycyrrhizic anhydride and auricularia auricula polysaccharide degradation products comprises the active ingredients of the glycyrrhizic anhydride and auricularia auricula polysaccharide degradation products; the weight ratio of the glycyrrhizic anhydride to the auricularia auricula polysaccharide degradation product is 1:1-1:10.
Preferably, the auricularia auricula polysaccharide degradation product is an acid degradation product obtained by extracting auricularia auricula through a water enzymatic method.
More preferably, the total sugar content of the auricularia auricula polysaccharide degradation product measured by a phenol-sulfuric acid method is 68.58%.
More preferably, the enzyme in the aqueous enzymatic extraction is pectase, and the weight ratio of pectase to tremella aurantialba powder is 1:10.
the compound composition also contains one or more of emulsifier, thickener, antiseptic, antibacterial agent, humectant, penetrating agent and whitening agent which can be added in preparing cosmetics.
Preferably, the weight ratio of the glycyrrhizic anhydride to the auricularia auricula polysaccharide degradation product is 1:5-1:8.
The application of the compound composition of the glycyrrhizic anhydride and the auricularia auricula polysaccharide degradation products in the preparation of moisturizing cosmetics.
Preferably, the dosage of the compound composition of the glycyrrhizic anhydride and the auricularia auricula polysaccharide degradation products is 0.1% -3%. The compound composition is added into cosmetics, and the moisturizing cosmetics are prepared according to the conventional preparation process.
The invention has the beneficial effects that compared with the prior art: in the research of the compatible pharmacological activity of Glycyrrhizin (GAMS) and auricularia auricula polysaccharide degradation products (TAP), the inventor finds that after the auricularia auricula is subjected to aqueous enzymatic extraction and acid degradation, the molecular weight of polysaccharide is reduced, so that the absorption of the polysaccharide is facilitated, and the GAMS and the TAP are combined for administration, especially when the weight ratio of the GAMS to the TAP is 1:1-1:10 has good moisturizing synergy when being matched; the inventor determines the in vitro moisturizing activity, and the protection effect of the sample on the dry and damaged HaCaT cells and the influence of the sample on the AQP3 content in the dry and damaged HaCaT cells show that the moisturizing composition has the effects of improving the self anti-drying capacity of the cells and enhancing the moisturizing effect of the skin on the molecular level, and meanwhile, has no toxic or side effect on the HaCaT cells. The moisturizing cream has good moisturizing effect through the measurement of skin hydration degree of healthy people at different times and the transepidermal water loss rate at different times by adding the moisturizing composition into cosmetic varieties.
Drawings
FIG. 1 skin hydration at various times
FIG. 2 transepidermal water loss at various times
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Experiment 1: preparation of auricularia auricula polysaccharide and degradation products thereof
Cleaning and drying fruiting body of Auricularia auricula (supplied by Xishuang et Zucc. Of China, and Tropical plant garden), pulverizing by a pulverizer, weighing 30g of the above dry powder, adding 10 times of water, adding 3g of pectase (purchased from Fluka company), performing enzymolysis at 35deg.C for 2h, adding 20 times of water, soaking in boiling water for 2h, centrifuging (7000 r/min,10 min), collecting supernatant, adding absolute ethanol to 80%, precipitating with 4 deg.C for 24h, centrifuging (3000 r/min,10 min), collecting precipitate, and oven drying to obtain Auricularia auricula polysaccharide. The tremella polysaccharide is prepared into solution, and the molecular weight of the tremella polysaccharide is about 12000kD through HPLC analysis.
0.6g of tremella aurantialba polysaccharide is taken according to the weight ratio of 1:30 mol/L HCl is added in, stirred evenly, degraded by magnetic stirring at 80 ℃ for 4 hours, centrifuged at 600 r/min for 10 minutes, and dried and ground in a drying oven at 65 ℃ for standby. The tremella aurantialba polysaccharide degradation product is prepared into a solution, and the molecular weight of the tremella aurantialba polysaccharide degradation product is about 67kD through HPLC analysis.
Experiment 2: determination of polysaccharide content
Adopts a phenol-sulfuric acid method and takes glucose as a standard substance.
And (3) manufacturing a standard curve: and (3) drying the glucose standard substance to constant weight at 105 ℃, taking 20mg, preparing the solution with ultrapure water, and fixing the volume to 100mL in a volumetric flask to obtain the glucose standard substance solution. The standard solution was diluted with ultrapure water to give a set of solutions with concentrations of 30, 50, 100, 150, 200. Mu.g/mL. Sucking 1.0mL of glucose solution, sequentially placing into 5 test tubes, respectively adding 0.5mL of 5% phenol and 2.5mL of concentrated sulfuric acid, mixing by shaking, standing at 25deg.C for 20min, and measuring absorbance at 490nm of spectrophotometer. And drawing a standard curve by taking the concentration of the glucose solution as an abscissa and the absorbance value as an ordinate. The method for measuring the sugar content in the auricular polysaccharides before and after degradation is the same as that of a standard substance, the polysaccharide after drying and constant weight is prepared into a solution, the absorbance value is measured at 490nm according to the method, and the polysaccharide content is calculated according to a standard curve. The total sugar content of the tremella aurantialba polysaccharide is about 54.38% measured by a phenol-sulfuric acid method, and the total sugar content of the tremella aurantialba polysaccharide degradation product is 68.58%.
Experiment 3: in vitro moisturizing Activity
In vitro moisturizing activity is detected by a weighing method, and saturated K is detected 2 CO 3 Solution and saturated (NH 4 )SO 4 Placing the solution in a sealed drier, placing the drier in a climatic chamber at 20deg.C to make the Relative Humidity (RH) of the drier be 43%, removing fat layer of fresh Corii Sus Domestica, dehairing with forceps, cleaning, and refrigerating at-20deg.C. The frozen pigskin was thawed in Phosphate Buffered Saline (PBS) at ph=7.4 for 30min. And (3) cutting the flat and intact pigskin into squares with the side length of about 1.3cm, then sucking 10 mu L of the prepared solution to be tested on the surface of the pigskin, immediately placing the solution in a dryer, and weighing the mass of the pigskin and the sample after 24 hours. In the experiment, pigskin without a sample is used as a blank control group, only Glycyrrhizin (GAMS) is coated as a comparative example 1, only auricular degradation polysaccharide (TAP) is coated as a comparative example 2, and only glycerol is coated as a comparative example 3. Each sample was measured 3 times in parallel, and an average was taken, and the formula of the moisture retention was as follows.
Wherein: h n -the mass of pigskin and sample after a period of time, g;
H 0 -mass of pig skin and sample without sample smeared, g.
Table 1: in vitro moisture retention of samples with different concentration ratios
The moisturizing composition of the present invention can be used in various applications in an amount of 0.1% to 3%. As can be seen from table 1, the moisturizing composition has a moisturizing effect in a weight ratio of 1:10, compared with the moisturizing rate of pigskin without the sample applied in the comparative example, wherein the effect is optimal in 1:8; compared with the common humectant glycerin, the moisturizing composition has the moisturizing effect basically equivalent to that of glycerin when the weight ratio is 1:3 and the dosage is 0.5 percent.
Experiment 4: effects of samples of different concentrations on HaCaT cytotoxicity
Cell Counting Kit-8 (CCK-8) is a colorimetric method with high sensitivity, no radioactivity and low cytotoxicity for measuring the number of living cells. The living cell dehydrogenase is continuously generated, WST-8 in the CCK-8 reagent is oxidized and reduced by the dehydrogenase in the presence of the Methoxy PMS electron carrier to generate orange-yellow formazan dye which can be dissolved in a culture medium, and the formazan amount is in direct proportion to the living cell number. I.e. the greater the number of living cells, the higher the o.d. value. Because the samples should be contacted with the skin to exert moisturizing effect, the CCK-8 method was used to evaluate the viability of HaCaT cells (the cell bank of the national academy of sciences of the classical culture collection committee) treated with samples of different mass concentrations in order to ensure the safety of the samples. The measuring method comprises the following steps:
diluting 10-30 generations of HaCaT cells in logarithmic phase with DMEM medium to 1×10 HaCaT cells 5 Cell suspension at concentration of each well, 100. Mu.L per well, was inoculated into 96-well plates, and cultured in 5% CO 2 Culturing in an incubator at 37 ℃ and saturated humidity for 24 hours, and removing the culture solution in the hole when the cell fusion degree reaches 80%. 100 mu L of sample solutions with different mass concentrations prepared by using DMEM culture medium are added into each hole, and the mixture is cultured for 24 hours and transferredThe medium was removed from the wells, washed 2 times with PBS, 100. Mu.L of 10% CCK-8 solution was added to each well, and incubated in an incubator protected from light for 1h. Immediately after the incubation, the OD at 450nm was measured with a microplate reader. Each group was set with 3 duplicate wells for averaging and the experiment was repeated 3 times.
In the experiment, wells containing DMEM medium and CCK-8 solution were used as control groups, and wells containing CCK-8 solution alone were used as blank groups.
Table 2: effects of samples of different concentrations on HaCaT cytotoxicity
As can be seen from table 2, the moisturizing composition has no toxic or side effect on HaCaT cells in an amount ranging from 0.1% to 3%, the cell viability is greater than 100%, and when the moisturizing composition is used in an amount of 2.0%, GAMS/tap=1: the survival rate of HaCaT cells at 8 was 120.21% at maximum.
Experiment 5: protection of samples against desiccated damaged HaCaT cells
A cell drying damage model is established, and the moisturizing activity of the substance is explored at the cellular level. The viability of dry injured HaCaT cells treated with samples of different mass concentrations was assessed using CCK-8 method. The method for establishing the cell drying damage model comprises the following steps: diluting 10-30 generations of HaCaT cells in logarithmic phase with DMEM medium to 1×10 HaCaT cells 5 Cell suspension at concentration of each well, 100. Mu.L per well, was inoculated into 96-well plates, and cultured in 5% CO 2 Culturing in an incubator at 37 ℃ and saturated humidity for 24 hours, and drying when the cell fusion degree reaches 80%. The drying treatment method comprises the following steps: placing the 96-well plate in a clean workbench, keeping the room temperature, and setting the drying air speed to be 0.4m/s; all culture solutions in the holes are sequentially removed, and the culture solutions are placed in a clean workbench for 20min, and HaCaT cell drying damage experiments show that the longer the cell drying time is, the smaller the cell volume is, and the lower the activity is. The cells were dried for 20min, with a viability of 50.45% approaching half-lethality. Then 100 mu L of a sample to be tested prepared by DMEM culture medium is added for culturing for 24 hours, and the concentration of the sample is a series of concentrations which are nontoxic to cells or have proliferation effect on the cells. In the experiment, the hole added with the DMEM medium only is used as a negative control group, and the positive control group is commercial grade ceramide CER-3 and auricularia auricula polysaccharide degradation product (TAP). The measurement method was the same as in experiment 4.
Table 3: protection of desiccated damaged HaCaT cells by samples at different concentrations
As can be seen from table 3, the moisturizing composition was used in an amount ranging from 0.1% to 3% for the damage treatment of HaCaT cells by drying and then loading, and had a damage repairing effect in the experimental concentration range, and in experiment 5, the moisturizing composition was used in an amount of 2.0% compared with experiment 4, GAMS/tap=1: the survival rate of HaCaT cells is maximum at 8 and is 102.01%, so that the damage repairing effect on the cells is closely related to the cell proliferation effect, the more obvious the sample has the cell proliferation effect, the stronger the capability of repairing damaged cells is, and the protection effect on the dry damaged HaCaT cells is obviously stronger than that of the commercial-grade ceramide CER-3.
Experiment 6: influence of the sample on the AQP3 content in dry damaged HaCaT cells
Diluting 10-30 generations of HaCaT cells in logarithmic phase with DMEM medium to 1×10 HaCaT cells 5 Inoculating cell suspension with concentration of each/hole into 6-well plate, culturing for 24 hr according to experiment 5, performing cell drying injury treatment, preparing serial sample concentrations with no cytotoxicity or cell proliferation effect with DMEM culture medium, adding sample group into different concentrations of sample to be tested, adding control group into DMEM culture medium and commercial grade ceramideCER-3, 2mL per well. After further incubation for 24h, the medium in wells was removed, each well was rinsed with 2mL of buffer, the 6 well plates were placed on ice, 1mL of buffer and 200 μl medium speed RIPA lysate were added to each well, and after complete lysis, the cell lysates were transferred to 1.5mL centrifuge tubes. Further disrupting the cells with an ultrasonic cell disruptor for 1min, centrifuging at 1000g for 3min, and storing the supernatant in a 2mL centrifuge tube for later use.
The enzyme-linked immunosorbent assay (ELISA) kit is used for measuring the AQP3 content in a specimen by using a double antibody sandwich method, the selected antibody is human aquaporin3, the stability and the plate-to-plate accuracy are good, the standard substance in the human aquaporin3 (AQP 3) ELISA kit (North NACHA Biotechnology Co., ltd.) is diluted into standard solutions of 2000, 1000, 500, 250 and 125ng/L, and the absorbance value of the cell lysate at 450nm is measured according to the description step operation.
Table 4: influence of the sample on the AQP3 content in dry damaged HaCaT cells
As can be seen from Table 4, the ELISA kit assay for the intracellular AQP3 content showed that examples 1-7 all up-regulate the AQP3 level in dry damaged HaCaT cells when the moisturizing composition was used at 2.0%, GAMS/TAP=1: 8 is up-regulated by 27.48% compared with the commercial-grade ceramide CER-3 of the comparative example 1, thus the experiment can prove that the moisturizing composition has the effects of improving the self anti-drying capability of cells and enhancing the moisturizing effect of skin on the molecular level.
Experiment 7: the composition is prepared into moisturizing cream according to the following formula:
the preparation process comprises the following steps: adding water phase except the moisturizing composition into a water phase pot, heating to 85 ℃ for dissolving for standby, adding the oil phase into an oil phase pot, heating to 80 ℃ for dissolving for standby, preheating the oil phase to 85 ℃ in an emulsifying pot, adding the water phase, adding the oil phase, homogenizing and emulsifying (3200R/min) for 5min, stirring (25R/min), vacuumizing to 0.4 atmosphere, slowly cooling to 45 ℃ for standby, adding the moisturizing composition, stirring (15R/min) for 15 min, discharging at 38 ℃ uniformly, and storing and standing.
Experiment 8, experiment 7 determination of moisturizing cream moisturizing Properties
30 volunteers aged 20-55 years were selected for skin health without cosmetic allergy history. Before the test, the left and right forearms are cleaned, dried, and at least stabilized for 30min in a constant temperature and humidity chamber with the relative humidity of 55% at the temperature of 20-22 ℃, after the skin temperature and humidity reach balance with the environment, test areas of 3cm multiplied by 3cm are respectively marked on the left and right forearms, the left arm is a control sample smearing area, and the right arm is a sample smearing area to be tested.
The TEWL change of the mark points on the inner side of the forearm of the subject before the sample was applied and after 10 and 120min was measured for 30s for each mark point using a Tewameter TM300 transepidermal water loss test probe, and the average was taken.
The stratum corneum moisture content of the test site was measured using a CM825 skin moisture test probe, and the average value of 5 measurements was taken as a test value, and the result was expressed as MMV. Then taking the moisturizing cream according to (2.0+/-0.1) mg sample/cm 2 Is applied to the test site, and MMV of the stratum corneum is measured in the same way after 10 and 120min, respectively. MMV change rate was calculated as per equation.
The high-efficiency moisturizing cream prepared in experiment 7 was subjected to measurement of skin hydration (MMV) and transepidermal water loss (TEWL), and the results are shown in fig. 1 and 2. As can be seen from fig. 1 and 2, the efficient moisturizing cream is a product with good instant moisturizing ability, and can better restore the barrier function of the skin. After use, the subjects consistently believe that the skin becomes soft and smooth, and can improve the dry skin condition.
While the foregoing is directed to the preferred embodiments of the present invention, it will be appreciated by those skilled in the art that changes and modifications may be made without departing from the principles of the invention, such changes and modifications are also intended to be within the scope of the invention.
Claims (5)
1. A compound composition of glycyrrhizic anhydride and auricularia auricula polysaccharide degradation products is characterized in that: the active ingredients of the compound composition consist of glycyrrhizic anhydride and auricularia auricula polysaccharide degradation products; the weight ratio of the glycyrrhizic anhydride to the auricularia auricula polysaccharide degradation products is 1:8;
the preparation method of the tremella aurantialba polysaccharide degradation product comprises the following steps: cleaning and drying tremella aurantialba fruiting bodies, crushing by a crusher, weighing 30g of the dry powder, adding 10 times of water, adding 3g of pectase, carrying out enzymolysis for 2 hours at 35 ℃, adding 20 times of water, carrying out boiling water immersion for 2 hours, centrifuging, 7000r/min,10min, taking supernatant, adding absolute ethanol to 80%, carrying out alcohol precipitation at 4 ℃ for 24h, centrifuging, 3000r/min,10min, taking precipitate, and drying to obtain tremella aurantialba polysaccharide;
0.6g of tremella aurantialba polysaccharide is taken according to the weight ratio of 1:30 mol/L HCl is added in, stirred evenly, degraded by magnetic stirring at 80 ℃ for 4 hours, 6000r/min, centrifuged for 10min, and dried and ground in a drying oven at 65 ℃ for standby.
2. The compound composition of glycyrrhizic anhydride and auricularia auricula polysaccharide degradation products according to claim 1, which is characterized in that: the total sugar content of the auricularia auricula polysaccharide degradation product measured by a phenol-sulfuric acid method is 68.58%.
3. The compound composition of glycyrrhizic anhydride and auricularia auricula polysaccharide degradation products according to claim 1, which is characterized in that: the compound composition also contains one or more of emulsifier, thickener, antiseptic, antibacterial agent, humectant, penetrating agent and whitening agent which can be added in preparing cosmetics.
4. Use of a compound composition of glycyrrhizic anhydride and auricularia auricula polysaccharide degradation products according to any one of claims 1-3 for the preparation of a moisturizing cosmetic.
5. The use of the compound composition of glycyrrhizic anhydride and auricularia auricular polysaccharide degradation products according to claim 4 for preparing a moisturizing cosmetic, characterized in that: the compound composition of the glycyrrhizic anhydride and the auricularia auricula polysaccharide degradation products is 0.1-3% in weight percentage.
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