CN113875901A

CN113875901A - Application of composition containing isochlorogenic acid in improvement of salpingitis of laying fowl

Info

Publication number: CN113875901A
Application number: CN202111142481.6A
Authority: CN
Inventors: 高伟; 宋伟光; 吴丽飞; 朱阵; 徐美利
Original assignee: Chenguang Biotech Group Co Ltd
Current assignee: Chenguang Biotech Group Co Ltd
Priority date: 2021-09-28
Filing date: 2021-09-28
Publication date: 2022-01-04

Abstract

The invention relates to the field of animal science, in particular to application of a composition containing isochlorogenic acid in improvement of salpingitis of laying fowl. The composition comprises isochlorogenic acid, flavonoid glycoside and terpenoid glycoside, can effectively inhibit the expression of proinflammatory factors and promote the expression of anti-inflammatory factors, and further can effectively prevent and treat salpingitis of laying fowls and improve the health condition of the laying fowls. Meanwhile, the product is pure natural, has no toxic or side effect, and can not cause the problems of flora imbalance, drug resistance enhancement, drug residue and the like. Therefore, the composition has good application prospect in the aspect of preventing and treating the salpingitis of the laying fowl.

Description

Application of composition containing isochlorogenic acid in improvement of salpingitis of laying fowl

Technical Field

The invention relates to the field of animal science, in particular to application of a composition containing isochlorogenic acid in improvement of salpingitis of laying fowl.

Background

The salpingitis of laying fowl is common disease of laying fowl, and is pathologically characterized in that inflammation reaction occurs at oviduct and uterus, and inflammation factors TNF-alpha, IFN-gamma, IL-6 and IL-8 of sick fowl are obviously increased. Clinically, the symptoms of the disease include reduced laying rate, reduced egg quality and the like. The salpingitis of the laying fowl not only affects the health condition of the laying fowl, but also affects the production performance of the laying fowl, leads to the death of the laying fowl when serious, and causes great economic loss to the breeding industry of the laying fowl.

Traditionally, antibiotics are mostly used for solving the problems, but the antibiotics have many side effects, such as unbalanced flora, drug resistance, drug residues and even harm to the livestock body and human health. And the quality requirements of egg products are continuously improved, so that more novel drugs or feed additives which are safe and nontoxic and can effectively prevent and treat the salpingitis of the laying fowls are urgently required to be developed.

Disclosure of Invention

The invention aims to provide a safe, non-toxic and purely natural isochlorogenic acid composition and application thereof in improving salpingitis of laying fowls.

In order to achieve the purpose, the technical scheme adopted by the invention is as follows:

the invention provides a composition and an application of any one of the following aspects:

(1) the application in preventing, slowing down or treating the salpingitis of the laying fowl;

(2) the application in preparing feed additive, animal feed or medicine; the feed additive, animal feed or medicament is used for preventing, slowing or treating salpingitis of laying fowl;

the composition comprises:

a first component which is isochlorogenic acid; and

a second component which is a mixture of flavonoid glycosides and terpenoid glycosides;

wherein the mass ratio of the first component to the second component is (5: 5) - (8: 2);

the mass ratio of the flavonoid glycoside to the terpenoid glycoside is (1-4): 1.

the invention unexpectedly discovers that after the isochlorogenic acid is compounded with the mixture of the flavonoid glycoside and the terpenoid glycoside in the proportion, the improvement effect of the isochlorogenic acid on the aspect of the salpingitis of the laying fowl can be synergistically improved.

Preferably, the mass ratio of the first component to the second component is (5.5: 4.5) - (8: 2).

Preferably, the mass ratio of the flavonoid glycoside to the terpenoid glycoside is (2-4): 1.

more preferably, the first component is contained in an amount of 50 wt.% or more, more preferably 70 wt.% or more, and still more preferably 80 wt.% or more, based on the total mass of the composition.

In the proportion, the composition can further effectively improve the effect of improving the salpingitis of the laying fowl.

Preferably, the composition comprises the following components in parts by weight: 50-80 parts of isochlorogenic acid, 5-20 parts of flavonoid glycoside and 5-20 parts of terpenoid glycoside.

Further preferably, the composition comprises the following components in parts by weight: 55-80 parts of isochlorogenic acid, 10-15 parts of flavonoid glycoside and 5-10 parts of terpenoid glycoside.

As a preferred embodiment, the composition comprises the following components in parts by weight: 80 parts of isochlorogenic acid, 15 parts of flavonoid glycoside and 5 parts of terpenoid glycoside.

Preferably, the isochlorogenic acid is selected from one or more of isochlorogenic acid A, isochlorogenic acid B and isochlorogenic acid C; more preferably, the isochlorogenic acid is prepared from (1-3) by mass: 1 of a mixture of isochlorogenic acid a and isochlorogenic acid C; further preferably, the isochlorogenic acid is prepared from the following components in a mass ratio of 2: 1 of iso-chlorogenic acid a and iso-chlorogenic acid C.

Preferably, the flavonoid glycoside is selected from one or more of flavonoids, flavonol compounds and isoflavonoids; more preferably, the flavonoid glycoside is prepared from (4-6) by mass: 1, wherein the flavonoids and the flavonols are both extracted from stevia rebaudiana; further preferably, the flavonoid glycoside is prepared from the following raw materials in a mass ratio of 5: 1 of flavonoids and flavonols.

In the present invention, the flavonoid and flavonol compounds are extracted from stevia rebaudiana Bertoni by conventional extraction methods, and the specific extraction method can be confirmed by those skilled in the art according to common knowledge.

Preferably, the terpenoid glycoside is stevioside.

The invention also discovers that when the specific selection of the isochlorogenic acid, the flavonoid glycoside and the terpenoid glycoside is defined as above, the synergistic effect of the composition in improving the salpingitis of the laying fowl can be further improved. Preferably, the composition is added to the animal feed or medicament in an amount of 0.01 wt.% to 0.04 wt.%; preferably 0.01 wt.% to 0.02 wt.%, more preferably 0.02 wt.%.

Preferably, the feed additive, animal feed or medicament is used to inhibit the expression of pro-inflammatory factors; preferably, the proinflammatory factors include one or more of TNF-alpha, IFN-gamma, IL-6, IL-8.

Preferably, the feed additive, animal feed or medicament is used to increase the expression of an anti-inflammatory factor; preferably, the anti-inflammatory factor comprises IL-10.

The laying fowl mentioned in the invention is preferably laying hen or laying duck.

In a preferred embodiment, the composition is administered to the laying fowl by means of a dressing or drinking water.

Further, the present invention also provides an animal feed or medicament containing 0.01 wt.% to 0.04 wt.% of a composition comprising:

a first component which is isochlorogenic acid; and

the mass ratio of the flavonoid glycoside to the terpenoid glycoside is (1-4): 1;

the feed additive, animal feed or medicament is used for preventing, slowing or treating salpingitis of laying fowl;

preferably, the composition is added to the animal feed or medicament in an amount of 0.01 wt.% to 0.02 wt.%, more preferably 0.02 wt.%.

Preferably, the mass ratio of the first component to the second component is (5.5: 4.5) - (8:

2)。

preferably, the proportion of the first component is more than 50 wt.% based on the total mass of the composition.

Preferably, the composition comprises the following components in parts by weight: 50-80 parts of isochlorogenic acid, 5-20 parts of flavonoid glycoside and 5-20 parts of terpenoid glycoside;

preferably, the composition comprises the following components in parts by weight: 55-80 parts of isochlorogenic acid, 10-15 parts of flavonoid glycoside and 5-10 parts of terpenoid glycoside;

more preferably, the composition comprises the following components in parts by weight: 80 parts of isochlorogenic acid, 15 parts of flavonoid glycoside and 5 parts of terpenoid glycoside.

Preferably, the terpenoid glycoside is stevioside.

Based on the technical scheme, the invention has the following beneficial effects:

the composition can effectively inhibit the expression of proinflammatory factors and promote the expression of anti-inflammatory factors, so that the laying fowl salpingitis can be effectively prevented and treated, and the health condition of the laying fowl can be improved. Meanwhile, the product is pure natural, has no toxic or side effect, and can not cause the problems of flora imbalance, drug resistance enhancement, drug residue and the like. Therefore, the composition has good application prospect in the aspect of preventing and treating the salpingitis of the laying fowl.

Drawings

FIG. 1 is a graph of the effect of a composition of the invention on the expression level of proinflammatory factors after modeling; in the figure, in a group of test results of each proinflammatory factor, the results of an amoxicillin group, an example 1, an example 2, an example 3, a model group and a control group are shown from left to right.

FIG. 2 is a graph of the effect of a composition of the invention on the expression level of proinflammatory factors; in the figure, the results of example 1, example 2, example 3, example 4, example 5, example 6 and the control group are shown from left to right in the set of test results for each proinflammatory factor.

FIG. 3 is a graph showing the effect of the composition of the present invention on the expression level of anti-inflammatory factor (IL-10).

Detailed Description

The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention.

In order to facilitate the comparison effect, the isochlorogenic acid mentioned in the following examples is 2 by mass: 1 of a mixture of isochlorogenic acid A and isochlorogenic acid C, wherein the flavonoid glycosides are all mixed according to a mass ratio of 5: 1 (both flavonoids and flavonols are extracted from stevia rebaudiana Bertoni by conventional extraction method), and the terpenoid glycosides are all stevioside (purchased from commercial sources). In the concrete implementation, the effects of the present invention can be achieved as long as the components used are within the range defined in the summary of the invention.

In order to facilitate the comparison effect, the same general ration is used in the following examples, and the specific formula is shown in table 1 below.

TABLE 1 basic diet formula and nutritional ingredients

Item	Content (%)	Nutritional index	Content (%)
				Corn (corn)	60.30	Metabolizable energy (MJ/kg)	11.60
Bean pulp	22.80	Crude protein (%)	16.78
				Wheat bran	4.20	Calcium (%)	3.67
Stone powder	9.35	Available phosphorus (%)	0.34
				Salt	0.30	Total phosphorus (%)	0.59
Soybean oil	0.85
				Calcium hydrogen phosphate	1.20
Premix compound	1.00
				Total up to	100.00

The examples do not show the specific techniques or conditions, according to the technical or conditions described in the literature in the field, or according to the product specifications. The reagents or instruments used are conventional products available from regular distributors, not indicated by the manufacturer.

Example 1

The embodiment provides a composition, which is composed of the following components in parts by weight: 80 parts of isochlorogenic acid, 15 parts of flavonoid glycoside and 5 parts of terpenoid glycoside.

The embodiment further provides a feed which comprises common daily ration and the composition, wherein the composition accounts for 0.01 percent of the total mass of the feed. During preparation, the composition is added into feed and mixed evenly to obtain the feed.

Example 2

This example provides a feed comprising a normal ration and the composition of example 1, wherein the composition comprises 0.02% of the total mass of the feed. During preparation, the composition is added into feed and mixed evenly to obtain the feed.

Example 3

This example provides a feed comprising a normal ration and the composition of example 1, wherein the composition comprises 0.04% of the total mass of the feed. During preparation, the composition is added into feed and mixed evenly to obtain the feed.

Example 4

The embodiment provides a composition, which is composed of the following components in parts by weight: 50 parts of isochlorogenic acid, 5 parts of flavonoid glycoside and 5 parts of terpenoid glycoside.

The embodiment further provides a feed which comprises common daily ration and the composition, wherein the composition accounts for 0.02% of the total mass of the feed. During preparation, the composition is added into feed and mixed evenly to obtain the feed.

Example 5

The embodiment provides a composition, which is composed of the following components in parts by weight: 80 parts of isochlorogenic acid, 20 parts of flavonoid glycoside and 20 parts of terpenoid glycoside.

Example 6

The embodiment provides a composition, which is composed of the following components in parts by weight: 80 parts of isochlorogenic acid, 20 parts of flavonoid glycoside and 5 parts of terpenoid glycoside.

Comparative example 1

This comparative example provides a feed which differs from example 2 only in that the flavonoid glycoside is replaced by an equal amount of terpenoid glycoside in the composition.

Comparative example 2

The present comparative example provides a feed which differs from example 2 only in that the formulation of the composition is different, and the composition of the present comparative example consists of the following components in parts by weight: 30 parts of isochlorogenic acid, 30 parts of flavonoid glycoside and 40 parts of terpenoid glycoside.

Test examples the effect of the composition of the present invention on the prevention and treatment of diseases of laying fowl

Experiment for constructing laying hen salpingitis model and verifying treatment effect

1.1 establishment of egg-laying hen salpingitis model

Preparing phenol mucilage: preparing 40mL of 25% phenol mucilage, measuring 10mL of liquefied phenol, 0.5g of sodium alginate and 8mL of glycerol, adding distilled water to 40mL, placing in a conical flask, and sealing for storage.

Preparation of salpingitis model: the laying hens are sequentially grabbed out, the two legs are respectively fixed by a ring finger, an index finger, a little finger and a thumb of a left hand, the palm centers of the hands are tightly attached to the tail ends of the sternums of the laying hens, then the hands are erected, the backs of the laying hens are tightly attached to the chests of the laying hens, the chicken heads face downwards, and the cloaca faces upwards. Then, the thumb and the 4 fingers of the right hand stretch across the two sides of the cloaca in a splayed shape, press down slightly, push upwards with the left hand supporting the breast of the hen at the same time, so that the mouth of the oviduct can be turned over, and the poison worker injects the phenol adhesive used for molding into the uterus of the oviduct, wherein 1mL of the phenol adhesive is used for each feather chicken.

Examination of salpingitis model: after the model is built, the laying hens have twitching, pain and uneasy sitting and lying, the eggshells of the produced eggs have bloodstains, and the number of inferior eggs is increased. The cloaca of the laying hens is congested and swollen, nearby feathers are polluted by yellow-white secretion, the abdomen of the laying hens is landed, and the laying hens are difficult to lay eggs.

And after the eggs are subjected to virus exposure for 24 hours, observing whether the symptoms exist, and performing virus exposure and molding again on the laying hens which are not successful in molding. And observing the egg laying condition of the laying hens for 48 hours, and counting the bad egg ratio.

1.2 test animals

Grouping laying hens: 360 feather laying hens in the laying period without obvious diseases are selected and randomly divided into 6 groups, each group contains 60 feathers, and the groups are divided into 3 repeats. Group I is feed added with antibiotic (amoxicillin); II. III and IV are respectively fed with the feed added in the groups 1, 2 and 3; group V is a model group, and only modeling is performed without treatment; group VI was a control group and was not treated.

1.3 serum factor detection

Serum anti-inflammatory factor and immunoglobulin: each group randomly selected 10-feather chickens, collected blood, centrifuged at 3000r/min for 10min at 4 deg.C, and serum was separated. The contents of TNF-alpha, IFN-gamma, IL-6 and IL-8 in serum are respectively detected by an ELISA method.

1.4 statistical analysis of data:

one-way analysis of variance was performed on all experimental data using SPSS 20 software.

1.5 test results

The results are shown in figure 1, and it can be seen from figure 1 that after the molding is successful, after the feed is used for one week, the amoxicillin groups, examples 1, 2 and 3 can obviously reduce the levels of proinflammatory factors TNF-alpha, IFN-gamma, IL-6 and IL-8.

Second, test for verifying treatment effect on salpingitis of laying hens in later period of laying

2 materials and methods

2.1 test drugs

Feed and regular ration of examples 1-6

2.2 test animals

The test chicken flock is 840 feathers of 480-day-old kalanchoe brown laying hens, which are averagely divided into 7 groups, wherein the group in example 1 is fed with the feed in example 1, the group in example 2 is fed with the feed in example 2, the group in example 3 is fed with the feed in example 3, the group in example 4 is fed with the feed in example 4, the group in example 5 is fed with the feed in example 5, the group in example 6 is fed with the feed in example 6, and the control group is fed with common daily ration without other epidemic prevention measures and the other feeding environments are consistent. The test period was 7 days.

2.3 serum factor detection

After the test is finished, 10 chickens are randomly selected in each group, blood is collected from veins under chicken wings, serum is separated, and the contents of serum anti-inflammatory factors TNF-alpha, IFN-gamma, IL-6, IL-8 and IL-10 are detected.

2.4 statistical analysis of data:

2.5 test results

The results are shown in fig. 2-3, and as can be seen from fig. 2 and 3, compared with the blank control group, the production performance of the laying hens in each example group is improved, the egg quality is obviously improved, the expression level of the proinflammatory factors in the example group is reduced, and the expression level of the anti-inflammatory factors is improved, which indicates that the composition can effectively inhibit the expression of the proinflammatory factors.

Meanwhile, the effect of the feed in the comparative example is verified by referring to the method, and the result shows that the effect test data of the feed in each embodiment has more outstanding advantages compared with the comparative example.

In conclusion, the composition of the invention can effectively prevent and treat the salpingitis of the laying fowl and improve the expression level of inflammatory factors. The amount of the composition of the present invention is preferably 0.02 wt.%.

Although the invention has been described in detail hereinabove with respect to a general description and specific embodiments thereof, it will be apparent to those skilled in the art that modifications or improvements may be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.

Claims

1. Use of a composition for the preparation of a feed additive, animal feed or medicament, wherein the composition comprises:

a first component which is isochlorogenic acid; and

the feed additive, animal feed or medicament is used for preventing, slowing down or treating the salpingitis of the laying fowl.

2. The use according to claim 1, wherein the mass ratio of the first component to the second component is (5.5: 4.5) - (8: 2); and/or

The mass ratio of the flavonoid glycoside to the terpenoid glycoside is (2-4): 1;

the proportion of the first component is preferably 50 wt.% or more, more preferably 70 wt.% or more, and still more preferably 80 wt.% or more, based on the total mass of the composition.

3. Use according to claim 1 or 2, wherein the composition comprises the following components in parts by weight: 50-80 parts of isochlorogenic acid, 5-20 parts of flavonoid glycoside and 5-20 parts of terpenoid glycoside;

4. The use according to any one of claims 1 to 3, wherein the isochlorogenic acid is selected from one or more of isochlorogenic acid A, isochlorogenic acid B, isochlorogenic acid C; preferably, the isochlorogenic acid is prepared from (1-3) by mass: 1 of a mixture of isochlorogenic acid a and isochlorogenic acid C;

and/or the flavonoid glycoside is selected from one or more of flavonoid compounds, flavonol compounds and isoflavone compounds; more preferably, the flavonoid glycoside is prepared from (4-6) by mass: 1, wherein the flavonoids and the flavonols are both extracted from stevia rebaudiana;

and/or the terpenoid glycoside is stevioside.

5. The use according to any one of claims 1 to 4, wherein the composition is added to the animal feed or medicament in an amount of 0.01 wt.% to 0.04 wt.%; preferably 0.01 wt.% to 0.02 wt.%, more preferably 0.02 wt.%.

6. Use according to any of claims 1-5, wherein the feed additive, animal feed or medicament is used in at least one of the following aspects:

(1) inhibiting the expression of a proinflammatory factor; preferably the proinflammatory factors comprise one or more of TNF-alpha, IFN-gamma, IL-6, IL-8;

(2) increasing the expression of anti-inflammatory factors; preferably, the anti-inflammatory factor comprises IL-10.

7. The use according to any one of claims 1 to 6, wherein the laying fowl is a laying hen or a laying duck.

8. An animal feed or medicament, characterized in that it contains 0.01 wt.% to 0.04 wt.% of a composition comprising:

a first component which is isochlorogenic acid; and

9. The animal feed or medicament of claim 8, wherein the mass ratio of the first component to the second component is (5.5: 4.5) - (8: 2); and/or

preferably, the proportion of the first component is more than 50 wt.% based on the total mass of the composition;

more preferably, the composition comprises the following components in parts by weight: 50-80 parts of isochlorogenic acid, 5-20 parts of flavonoid glycoside and 5-20 parts of terpenoid glycoside.

10. The animal feed or medicament of claim 8 or 9, wherein the isochlorogenic acid is selected from one or more of isochlorogenic acid A, isochlorogenic acid B, isochlorogenic acid C; preferably, the isochlorogenic acid is prepared from (1-3) by mass: 1 of a mixture of isochlorogenic acid a and isochlorogenic acid C;

and/or the terpenoid glycoside is stevioside.