CN113854347B - Composite antifreeze agent suitable for euphausia superba and application thereof - Google Patents
Composite antifreeze agent suitable for euphausia superba and application thereof Download PDFInfo
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- CN113854347B CN113854347B CN202111175215.3A CN202111175215A CN113854347B CN 113854347 B CN113854347 B CN 113854347B CN 202111175215 A CN202111175215 A CN 202111175215A CN 113854347 B CN113854347 B CN 113854347B
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- 239000002131 composite material Substances 0.000 title claims abstract description 102
- 239000007798 antifreeze agent Substances 0.000 title claims abstract description 97
- 241000239370 Euphausia superba Species 0.000 title claims abstract description 45
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 44
- 238000000034 method Methods 0.000 claims abstract description 31
- 241000239366 Euphausiacea Species 0.000 claims abstract description 30
- 238000007710 freezing Methods 0.000 claims abstract description 21
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims abstract description 19
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 claims abstract description 19
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 claims abstract description 18
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 claims abstract description 18
- 239000001509 sodium citrate Substances 0.000 claims abstract description 18
- 239000004475 Arginine Substances 0.000 claims abstract description 13
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 claims abstract description 13
- 239000004472 Lysine Substances 0.000 claims abstract description 13
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 claims abstract description 13
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 claims abstract description 13
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 claims abstract description 13
- 230000002528 anti-freeze Effects 0.000 claims description 42
- 235000013372 meat Nutrition 0.000 claims description 24
- 238000002791 soaking Methods 0.000 claims description 21
- 239000003795 chemical substances by application Substances 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 2
- 238000005138 cryopreservation Methods 0.000 claims 2
- 239000002994 raw material Substances 0.000 abstract description 16
- 238000010257 thawing Methods 0.000 abstract description 16
- 230000008014 freezing Effects 0.000 abstract description 15
- 102000004169 proteins and genes Human genes 0.000 abstract description 12
- 108090000623 proteins and genes Proteins 0.000 abstract description 12
- 238000004925 denaturation Methods 0.000 abstract description 8
- 230000036425 denaturation Effects 0.000 abstract description 8
- 238000003860 storage Methods 0.000 abstract description 7
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 abstract description 6
- 229930006000 Sucrose Natural products 0.000 abstract description 6
- 239000005720 sucrose Substances 0.000 abstract description 6
- 230000008569 process Effects 0.000 abstract description 5
- 229910019142 PO4 Inorganic materials 0.000 abstract description 4
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 abstract description 4
- 239000010452 phosphate Substances 0.000 abstract description 4
- 230000000052 comparative effect Effects 0.000 description 22
- 230000000694 effects Effects 0.000 description 20
- 239000000203 mixture Substances 0.000 description 16
- 239000000047 product Substances 0.000 description 16
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 description 12
- HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 description 12
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 description 12
- 235000018102 proteins Nutrition 0.000 description 10
- 150000001875 compounds Chemical class 0.000 description 7
- 238000005119 centrifugation Methods 0.000 description 4
- 238000013329 compounding Methods 0.000 description 4
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 229920002752 Konjac Polymers 0.000 description 3
- 230000036541 health Effects 0.000 description 3
- 238000004321 preservation Methods 0.000 description 3
- 239000000600 sorbitol Substances 0.000 description 3
- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 description 2
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 2
- 241001122767 Theaceae Species 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000013078 crystal Substances 0.000 description 2
- 235000013312 flour Nutrition 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 238000009472 formulation Methods 0.000 description 2
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 2
- 230000002641 glycemic effect Effects 0.000 description 2
- 238000009776 industrial production Methods 0.000 description 2
- 235000010485 konjac Nutrition 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 239000011574 phosphorus Substances 0.000 description 2
- 229910052698 phosphorus Inorganic materials 0.000 description 2
- 150000008442 polyphenolic compounds Chemical class 0.000 description 2
- 235000013824 polyphenols Nutrition 0.000 description 2
- 235000019832 sodium triphosphate Nutrition 0.000 description 2
- 239000001577 tetrasodium phosphonato phosphate Substances 0.000 description 2
- 239000002028 Biomass Substances 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 241000238557 Decapoda Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 208000008589 Obesity Diseases 0.000 description 1
- ZQBZAOZWBKABNC-UHFFFAOYSA-N [P].[Ca] Chemical compound [P].[Ca] ZQBZAOZWBKABNC-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 235000021120 animal protein Nutrition 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 235000012041 food component Nutrition 0.000 description 1
- 239000005417 food ingredient Substances 0.000 description 1
- 230000005802 health problem Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 235000020824 obesity Nutrition 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000012466 permeate Substances 0.000 description 1
- 150000003013 phosphoric acid derivatives Chemical class 0.000 description 1
- 239000011814 protection agent Substances 0.000 description 1
- 239000008213 purified water Substances 0.000 description 1
- 238000005057 refrigeration Methods 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- FQENQNTWSFEDLI-UHFFFAOYSA-J sodium diphosphate Chemical compound [Na+].[Na+].[Na+].[Na+].[O-]P([O-])(=O)OP([O-])([O-])=O FQENQNTWSFEDLI-UHFFFAOYSA-J 0.000 description 1
- GCLGEJMYGQKIIW-UHFFFAOYSA-H sodium hexametaphosphate Chemical compound [Na]OP1(=O)OP(=O)(O[Na])OP(=O)(O[Na])OP(=O)(O[Na])OP(=O)(O[Na])OP(=O)(O[Na])O1 GCLGEJMYGQKIIW-UHFFFAOYSA-H 0.000 description 1
- 235000019982 sodium hexametaphosphate Nutrition 0.000 description 1
- 229940048086 sodium pyrophosphate Drugs 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 235000019818 tetrasodium diphosphate Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B4/00—General methods for preserving meat, sausages, fish or fish products
- A23B4/14—Preserving with chemicals not covered by groups A23B4/02 or A23B4/12
- A23B4/18—Preserving with chemicals not covered by groups A23B4/02 or A23B4/12 in the form of liquids or solids
- A23B4/20—Organic compounds; Microorganisms; Enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Meat, Egg Or Seafood Products (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention provides a composite antifreeze agent suitable for antarctic krill and application thereof, and the antifreeze agent comprises the following raw materials in percentage by mass: 10-30% of arginine, 10-30% of lysine, 10-30% of proline, 10-30% of histidine, 5-15% of trehalose and 5-15% of sodium citrate. The composite antifreeze agent disclosed by the invention does not contain phosphate and sucrose, is high in safety and low in sweetness, is quick and convenient to use, is combined with terahertz waves to soak the euphausia superba after being dissolved in water, can weaken the protein denaturation of the euphausia superba in a long-time freezing storage process, reduces the thawing loss of the euphausia superba, and simultaneously improves the water holding and processing characteristics of the euphausia superba after freezing storage.
Description
Technical Field
The invention relates to the field of aquatic products, in particular to a composite antifreeze agent suitable for euphausia superba and application thereof.
Background
Euphausia superba is the single organism with the most abundant known resource content living on the earth at the antarctic, and is rich in nutrition, called the largest animal protein pool on the earth. It is counted that the biomass of antarctic krill is about 6-10 hundred million tons, and about 3.42-5.36 hundred million tons of larvae can be bred each year. The fishing of the antarctic krill starts from the year 60 of the 20 th century abroad, and the fishing work of the antarctic krill is carried out for the first time in 2009 in China. Because the fishing place is far in the antarctic sea area, and antarctic krill is rich in low-temperature autolyzed enzyme, rapid freezing after fishing is an important method for preserving antarctic krill. However, due to the distance factor of the antarctic krill fishing place, the freezing storage time of the antarctic krill is far longer than that of a common aquatic product, and in the long-term freezing storage process, the growth of a large number of irregular ice crystals can influence the combination state between protein molecules and combined water, and part of combined water is separated and converted into ice crystals, so that the interaction between water and protein is caused, the original tissue structure of the protein is influenced, the water retention capacity is reduced due to protein denaturation, and the thawing yield and the product processing quality of the antarctic krill are influenced.
Antifreeze agents, which are substances added to foods to prevent the foods from being damaged by freezing to achieve a long-term preservation effect, are used today with a large amount of antifreeze agents, such as phosphate and sucrose or sorbitol, as glycerol is added to aquatic products at the earliest in japan to prevent protein denaturation in the aquatic products. The phosphate salt such as sodium tripolyphosphate, sodium hexametaphosphate, sodium pyrophosphate or a mixture thereof is used as a freezing protection agent for aquatic products, the adding amount of the phosphate needs to be strictly controlled, and the additive effect generated by eating various aquatic products for a long time can cause excessive phosphorus intake to influence the balance of calcium and phosphorus of human bodies and harm health; sucrose and sorbitol are unfavorable for obese patients and diabetics because of their high sweetness and high glycemic index. Therefore, in consideration of the current healthy consumption trend and certain special crowds, the development of a novel green healthy composite antifreeze agent suitable for antarctic krill has great significance.
Terahertz wave is specifically designated 10 11 ～10 13 The Hz frequency band has the wavelength of 30 um-3 mm, electromagnetic waves between microwaves and infrared rays, water molecules, water molecular groups, various microscopic particles, molecules and the like absorb terahertz waves and generate same-frequency resonance with self vibration waves, and the water dissolving capacity is enhanced under the action of the same-frequency resonance waves.
Disclosure of Invention
The invention provides a compound antifreeze agent suitable for euphausia superba and application thereof, which aims to solve the problems that protein denaturation and processing quality are reduced due to long-time freezing preservation of aquatic products such as euphausia superba, and calcium-phosphorus balance of human bodies is influenced to harm health, obesity patients and diabetes mellitus are not benefited, and the like.
In order to achieve the above purpose, the technical scheme adopted by the invention is as follows:
a composite antifreeze agent suitable for antarctic krill comprises the following components in percentage by mass:
in a preferred mode, the composite antifreeze agent suitable for euphausia superba consists of the following components in percentage by mass:
the application of the composite antifreeze agent for the euphausia superba is that euphausia superba meat is soaked by using the composite antifreeze agent for the euphausia superba, so that the freezing denaturation of euphausia superba protein is slowed down, and the processing quality is improved; the specific application method comprises the following steps: soaking euphausia superba meat in the composite antifreeze solution for 0.5-2h at 4 ℃ and combining terahertz wave treatment; the composite antifreeze solution comprises the following components: the compound antifreeze agent suitable for the euphausia superba is dissolved in water to prepare the compound antifreeze agent; wherein the mass ratio of the composite antifreeze agent to the water suitable for the euphausia superba is 1:19; the mass ratio of the euphausia superba meat to the composite antifreeze solution is 1:3-3:1; the terahertz wave frequency is 0.1-0.3THz, the power is 50-100mW, and the treatment time is 0.5-2h.
The invention has the beneficial effects that:
(1) The composite antifreeze agent suitable for the euphausia superba, disclosed by the invention, can weaken protein freezing denaturation in the freezing preservation process, improve water retention, reduce thawing loss, delay degradation of euphausia superba meat quality and improve processing characteristics when being used for freezing pretreatment of the euphausia superba. Compared with a control group without any antifreeze agent, the thawing loss rate of the preferred scheme is relatively reduced by 73.9%, and the water holding rate is relatively improved by 28.6%.
(2) The composite antifreeze agent suitable for euphausia superba has safe and phosphorus-free components, can avoid the health problem caused by adding phosphate and sucrose, has low sweetness, can be suitable for various aquatic products, has universality and is beneficial to the health of consumers.
(3) The composite antifreeze agent suitable for the antarctic krill adopts a terahertz auxiliary method in application, and molecular movement is activated based on terahertz wave energy, so that the antifreeze agent can better permeate into shrimp meat tissues, and the antifreeze effect is enhanced. Compared with the method that the terahertz wave is not used and the composite antifreeze agent is directly used, the thawing loss rate is relatively reduced by 21.4%, and the water retention rate is relatively increased by 3.2%.
Detailed Description
The following describes the embodiments of the present invention in further detail with reference to examples.
Arginine used in the following examples was manufactured by Henan Tang style food ingredients Co., ltd, CAS number 74-79-3; lysine, manufacturer Henan Tanggou food ingredient Co., ltd, CAS number 56-87-1; proline is manufactured by Henan Tanggou food ingredients limited company, and the CAS number is 147-85-3; histidine, henan Tanggou food ingredient Co., ltd, CAS number 71-00-1; trehalose, manufacturer is Nanjing Beijing Runfu biotechnology Co., ltd, CAS number is 99-20-7; sodium citrate, manufacturer is Shandong Weifang Yingxuan Utility Co., ltd, CAS number 6132-04-3; konjak flour is manufactured by Hubei Qianson konjak technology Co., ltd, and the CAS number is 37220-17-0; tea polyphenols, the manufacturer is Shandong Xingshi Biotechnology Co., ltd, with CAS number 84650-60-2.
A composite antifreeze agent suitable for antarctic krill comprises the following components in percentage by mass: 10-30% of arginine, 10-30% of lysine, 10-30% of proline, 10-30% of histidine, 5-15% of trehalose and 5-15% of sodium citrate.
The application method of the composite antifreeze agent suitable for the antarctic krill comprises the following steps:
s1, uniformly mixing the composite antifreeze agent with water according to a mass ratio of 1:19, and fully dissolving to prepare a composite antifreeze agent solution;
s2, soaking euphausia superba meat in the composite anti-freezing agent solution according to the mass ratio of 1:3-3:1, and soaking for 0.5-2h at the temperature of 4-15 ℃.
S3, processing the terahertz waves for 0.5-2h with the frequency of 0.1-0.3THz and the power of 50-100mW while performing the step S2.
In the step S3, the terahertz wave processor is placed at the bottom of the soaking liquid.
Example 1
The invention discloses a composite antifreeze agent suitable for antarctic krill, which comprises the following raw materials in percentage by mass: 10% of arginine, 30% of lysine, 30% of proline, 10% of histidine, 15% of trehalose and 5% of sodium citrate.
The application method comprises the following specific implementation steps:
(1) 50g of the composite antifreeze agent suitable for the euphausia superba is added with 950g of water, and is uniformly mixed and fully dissolved to prepare a composite antifreeze agent solution;
(2) 500g of euphausia superba meat is soaked in the compound anti-freezing agent solution at 4 ℃ for 1h.
Example 2
The invention discloses a composite antifreeze agent suitable for antarctic krill, which comprises the following raw materials in percentage by mass: 10% of arginine, 10% of lysine, 30% of proline, 30% of histidine, 10% of trehalose and 10% of sodium citrate.
The application method comprises the following specific implementation steps:
(1) 50g of the composite antifreeze agent is added with 950g of water, and is uniformly mixed and fully dissolved to prepare a composite antifreeze agent solution;
(2) 500g of euphausia superba meat is soaked in the composite antifreeze solution at 15 ℃ for 1h.
(3) When the composite antifreeze solution is used for soaking, terahertz wave treatment is used, a terahertz wave processor is placed at the bottom of the soaking solution, the terahertz frequency is 0.1THz, the power is 80mW, and the treatment time is 1h.
Example 3
The invention discloses a composite antifreeze agent suitable for antarctic krill, which comprises the following raw materials in percentage by mass: 10% of arginine, 30% of lysine, 30% of proline, 10% of histidine, 15% of trehalose and 5% of sodium citrate.
The application method comprises the following specific implementation steps:
(1) 50g of the composite antifreeze agent is added with 950g of water, and the mixture is fully dissolved to prepare a composite antifreeze agent solution;
(2) 500g of euphausia superba meat is soaked in the compound anti-freezing agent solution at 4 ℃ for 1h.
(3) When the composite antifreeze solution is used for soaking, terahertz wave treatment is used, a terahertz wave processor is placed at the bottom of the soaking solution, the terahertz frequency is 0.3THz, the power is 80mW, and the treatment time is 1h.
Example 4
The invention discloses a composite antifreeze agent suitable for antarctic krill, which is prepared by compounding the following raw materials in percentage by mass: 20% of arginine, 20% of lysine, 20% of proline, 20% of histidine, 5% of trehalose and 15% of sodium citrate.
The application method comprises the following specific implementation steps:
(1) 50g of the composite antifreeze agent is added with 950g of water, and the mixture is fully dissolved to prepare a composite antifreeze agent solution;
(2) 200g of euphausia superba meat is soaked in the composite antifreeze solution at 4 ℃ for 2 hours.
(3) When the composite antifreeze solution is used for soaking, terahertz wave treatment is used, a terahertz wave processor is placed at the bottom of the soaking solution, the terahertz frequency is 0.1THz, the power is 100mW, and the treatment time is 2 hours.
Example 5
The invention discloses a composite antifreeze agent suitable for antarctic krill, which is prepared by compounding the following raw materials in percentage by mass: 30% of arginine, 30% of lysine, 20% of proline, 10% of histidine, 5% of trehalose and 5% of sodium citrate.
The application method comprises the following specific implementation steps:
(1) 50g of the composite antifreeze agent is added with 950g of water, and the mixture is fully dissolved to prepare a composite antifreeze agent solution;
(2) 500g of euphausia superba meat is soaked in the compound anti-freezing agent solution for 2 hours at 4 ℃.
(3) When the composite antifreeze solution is used for soaking, terahertz wave treatment is used, a terahertz wave processor is placed at the bottom of the soaking solution, the terahertz frequency is 0.2THz, the power is 80mW, and the treatment time is 2h.
Example 6
The invention discloses a composite antifreeze agent suitable for antarctic krill, which is prepared by compounding the following raw materials in percentage by mass: 20% of arginine, 20% of lysine, 10% of proline, 20% of histidine, 15% of trehalose and 15% of sodium citrate.
The application method comprises the following specific implementation steps:
(1) 50g of the composite antifreeze agent is added with 950g of water, and the mixture is fully dissolved to prepare a composite antifreeze agent solution;
(2) 2000g of euphausia superba meat is soaked in the composite antifreeze solution at 4 ℃ for 0.5h.
(3) When the composite antifreeze solution is used for soaking, terahertz wave treatment is used, a terahertz wave processor is placed at the bottom of the soaking solution, the terahertz frequency is 0.3THz, the power is 100mW, and the treatment time is 0.5h.
Example 7
The invention discloses a composite antifreeze agent suitable for antarctic krill, which is prepared by compounding the following raw materials in percentage by mass: 30% of arginine, 10% of lysine, 10% of proline, 30% of histidine, 15% of trehalose and 5% of sodium citrate.
The application method comprises the following specific implementation steps:
(1) 50g of the composite antifreeze agent is added with 950g of water, and the mixture is fully dissolved to prepare a composite antifreeze agent solution;
(2) 333g of euphausia superba meat is soaked in the composite antifreeze solution at 10 ℃ for 1h.
(3) When the composite antifreeze solution is used for soaking, terahertz wave treatment is used, a terahertz wave processor is placed at the bottom of the soaking solution, the terahertz frequency is 0.2THz, the power is 50mW, and the treatment time is 1h.
Comparative example 1
The composite antifreeze agent with the antifreeze effect is prepared from the following raw materials in percentage by mass: sodium citrate 100%.
The application method of the composite antifreeze agent with the antifreeze effect comprises the following specific implementation steps:
(1) 50g of the composite antifreeze agent is added with 950g of water, and the mixture is fully dissolved to prepare a composite antifreeze agent solution;
(2) 500g of euphausia superba meat is soaked in the composite antifreeze solution at 4 ℃ for 1h.
Comparative example 2
The composite antifreeze agent with the antifreeze effect is prepared from the following raw materials in percentage by mass: 50% of trehalose and 50% of sodium citrate.
The application method of the composite antifreeze agent with the antifreeze effect comprises the following specific implementation steps:
(1) 50g of the composite antifreeze agent is added with 950g of water, and the mixture is fully dissolved to prepare a composite antifreeze agent solution;
(2) 500g of euphausia superba meat is soaked in the composite antifreeze solution at 4 ℃ for 1h.
Comparative example 3
The composite antifreeze agent with the antifreeze effect is prepared from the following raw materials in percentage by mass: arginine 60%, trehalose 20% and sodium citrate 20%.
The application method of the composite antifreeze agent with the antifreeze effect comprises the following specific implementation steps:
(1) 50g of the composite antifreeze agent is added with 950g of water, and the mixture is fully dissolved to prepare a composite antifreeze agent solution;
(2) 500g of euphausia superba meat is soaked in the composite antifreeze solution at 4 ℃ for 1h.
Comparative example 4
The composite antifreeze agent with the antifreeze effect is prepared from the following raw materials in percentage by mass: 60% of lysine, 20% of trehalose and 20% of sodium citrate.
The application method of the composite antifreeze agent with the antifreeze effect comprises the following specific implementation steps:
(1) 50g of the composite antifreeze agent is added with 950g of water, and the mixture is fully dissolved to prepare a composite antifreeze agent solution;
(2) 500g of euphausia superba meat is soaked in the composite antifreeze solution at 4 ℃ for 1h.
Comparative example 5
The composite antifreeze agent with the antifreeze effect is prepared from the following raw materials in percentage by mass: proline 60%, trehalose 20% and sodium citrate 20%.
The application method of the composite antifreeze agent with the antifreeze effect comprises the following specific implementation steps:
(1) 50g of the composite antifreeze agent is added with 950g of water, and the mixture is fully dissolved to prepare a composite antifreeze agent solution;
(2) 500g of euphausia superba meat is soaked in the composite antifreeze solution at 4 ℃ for 1h.
Comparative example 6
The composite antifreeze agent with the antifreeze effect is prepared from the following raw materials in percentage by mass: 60% of histidine, 20% of trehalose and 20% of sodium citrate.
The application method of the composite antifreeze agent with the antifreeze effect comprises the following specific implementation steps:
(1) 50g of the composite antifreeze agent is added with 950g of water, and the mixture is fully dissolved to prepare a composite antifreeze agent solution;
(2) 500g of euphausia superba meat is soaked in the composite antifreeze solution at 4 ℃ for 1h.
Comparative example 7
A commercial aquatic product antifreeze agent has a brand name of various flavors and a product model number BXF01.
The application method of the commercial aquatic product antifreeze comprises the following specific implementation steps:
(1) 10g of the commercial aquatic product antifreeze agent is added with 990g of water, and the mixture is fully dissolved to prepare an aqueous product antifreeze agent solution;
(2) 500g of euphausia superba meat is soaked in the aquatic product antifreeze solution for 15min at 4 ℃.
Comparative example 8
The traditional antifreeze agent with the antifreeze effect is prepared from the following raw materials in percentage by mass: 48.8% of sucrose, 48.8% of sorbitol and 2.4% of sodium tripolyphosphate.
The application method of the composite antifreeze agent with the antifreeze effect comprises the following specific implementation steps:
(1) 82g of the traditional antifreeze agent is added with 918g of water, and is uniformly mixed and fully dissolved to prepare a traditional antifreeze agent solution;
(2) 500g of euphausia superba meat is soaked in a traditional antifreeze solution at 4 ℃ for 1h.
Comparative example 9
The composite antifreeze agent with the antifreeze effect is prepared from the following raw materials in percentage by mass: 10% of arginine, 20% of lysine, 20% of proline, 10% of histidine, 15% of trehalose, 5% of sodium citrate, 10% of konjaku flour and 10% of tea polyphenol.
The application method of the composite antifreeze agent with the antifreeze effect comprises the following specific implementation steps:
(1) 50g of the composite antifreeze agent is added with 950g of water, and the mixture is fully dissolved to prepare a composite antifreeze agent solution;
(2) 500g of euphausia superba meat is soaked in the composite antifreeze solution at 4 ℃ for 1h.
Comparative example 10
The specific implementation steps are as follows: 500g of euphausia superba meat is soaked in 1000g of solution, soaked for 1h at 4 ℃, and the water is drained after soaking; the solution is purified water without any antifreeze component.
The euphausia superba soaked and drained in all the examples and comparative examples of the invention is taken as a sample to be studied, and the euphausia superba is placed in a refrigeration house at-18 ℃ for freezing and storing for 3 months.
And (3) measuring indexes:
(1) Thawing loss rate: namely, each sample was completely thawed at 4℃after 3 months of frozen storage, and the quality was changed before and after thawing. The smaller the defrost loss, the less juice loss, and the lower the loss.
Rate of thawing loss = (mass before thawing-mass after thawing)/mass before thawing×100%
(2) Water retention capacity: namely, each sample was completely thawed at 4℃after 3 months of frozen storage, surface moisture was wiped off, and centrifuged at 3000g for 20min, and the mass was changed before and after centrifugation. The smaller the mass change value before and after centrifugation, the larger the water holding capacity, which shows that the better the water holding capacity of the antarctic krill after frozen storage.
Water retention = post-centrifugation mass/pre-centrifugation mass x 100%
The data on thawing loss and water retention reflect the extent of protein denaturation from the side.
Thus, table 1 was prepared: thawing loss rate and water holding ability of each of examples and comparative examples
Table 1: thawing loss rate and water holding ability of each of examples and comparative examples
Comparative example 8 is a conventional commonly used antifreeze agent, comparative example 7 is a commercial antifreeze agent, and the literature shows that trehalose has about 45% of the sweetness of sucrose, and has slow absorption in vivo and a gentle glycemic response.
As can be seen from table 1, after 3 months of freezing, the samples treated by each example of the present invention were controlled to have a thawing loss rate of 10% or less, which is generally lower than that of each comparative example; the water retention is above 70%, generally higher than that of the comparative example. From the data of the thawing loss rate and the water holding capacity, the optimal example is the example 3, the thawing loss rate is 6.6%, the water holding capacity is far lower than that of the comparative example 10 serving as a control, the water holding capacity is 77.3%, the water holding capacity is far higher than that of the comparative example 10 serving as a control group, and the water holding capacity is superior to that of an antifreeze agent (comparative example 8) and a commercial antifreeze agent (comparative example 7) of the traditional formula, and meanwhile, the optimal example has a remarkable enhancement effect compared with the example 1 which is prepared by the same formula but is not subjected to terahertz treatment. From the standpoint of formulation rationality and industrial production cost, the best example is also example 3, while the formulation is more complex, and the effect of comparative example 9, with more different components added, is not remarkable, and is less advantageous than example 3.
Therefore, the compound antifreeze agent suitable for the antarctic krill and the application thereof can better slow down protein denaturation in the antarctic krill freezing process, protect a protein structure, reduce own juice loss and enhance water holding capacity; the method can reduce cost loss in industrial production and transportation processes, improve processing characteristics, improve processing quality of subsequent products, and has the characteristics of safe components, low sweetness and simple use method.
The foregoing is only a preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art, who is within the scope of the present invention, should be covered by the protection scope of the present invention by making equivalents and modifications to the technical solution and the inventive concept thereof.
Claims (6)
1. The composite antifreeze agent suitable for the antarctic krill is characterized by comprising the following components in percentage by mass: 10% of arginine, 30% of lysine, 30% of proline, 10% of histidine, 15% of trehalose and 5% of sodium citrate.
2. Use of a composite antifreeze agent for antarctic krill as claimed in claim 1, for the cryopreservation of aquatic products.
3. Use of a composite antifreeze agent for antarctic krill according to claim 2, for the cryopreservation of antarctic krill.
4. A method of using the composite antifreeze agent of claim 1 for antarctic krill, comprising the steps of:
s1, uniformly mixing the composite antifreeze agent with water, and fully dissolving to prepare a composite antifreeze agent solution;
s2, soaking the aquatic product in the composite anti-freezing agent solution;
s3, processing by using terahertz waves is performed in step S2.
5. The method for using the composite antifreeze agent for antarctic krill according to claim 4, comprising the following steps:
s1, uniformly mixing the composite antifreeze agent with water according to a mass ratio of 1:19, and fully dissolving to prepare a composite antifreeze agent solution;
s2, soaking euphausia superba meat in the composite anti-freezing agent solution according to the mass ratio of 1:3-3:1, and soaking for 0.5-2h at the temperature of 4-15 ℃;
s3, processing the terahertz wave for 0.5-2 hours with the frequency of 0.1-0.3THz and the power of 50-100mW at the same time of the step S2.
6. The method for using the composite antifreeze according to claim 4 or 5, wherein: in the step S3, the terahertz wave processor is placed at the bottom of the soaking liquid.
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