CN113841807A - Preparation for preventing probiotics liquid from blocking waterline and preparation method thereof - Google Patents

Abstract

A preparation for preventing probiotic liquid from blocking a water line belongs to the technical field of veterinary preparations, and is prepared from 100g of the preparation by weight: 1.0-5.0 g of azulene, 1.0-10.0 g of sesbania gum, 901.0-15.0 g of polyvinylpyrrolidone K, 0.1-2.0 g of calcium chloride, 1.0-6.0 g of propylene glycol alginate, 1.0-20.0 g of glyceryl triacetate, 0.1-2.0 g of aluminum stearate and the balance of deionized water. The preparation is mainly used in combination with probiotic liquid clinically, can prevent the probiotic liquid from blocking a drinking water pipeline, avoids waste of manpower and material resources caused by cleaning the pipeline, can ensure uniform drinking water administration of the probiotic liquid, and improves bioavailability. Meanwhile, the invention further discloses a preparation method of the compound, which has strong process operability, is beneficial to production conversion and has good market prospect.

Description

Preparation for preventing probiotics liquid from blocking waterline and preparation method thereof

Technical Field

The invention belongs to the technical field of veterinary drug preparations, and particularly relates to a preparation for preventing probiotics from blocking a waterline and a preparation method thereof.

Background

The probiotics are one of the common feed additives for veterinary clinical use, belong to the field of micro-ecology, and the preparation products take the probiotics, the prebiotics and the like as main components and are mainly used for clinically preventing and controlling livestock and poultry diseases, promoting growth, reducing breeding pollution, improving animal production performance and the like. The products of the prior product comprise a liquid preparation and a solid preparation, wherein strains contained in the liquid preparation are mainly strains which can not form spores, such as lactobacillus plantarum, enterococcus lactis, enterococcus faecalis and the like. Because the solid preparation lacks moisture, the probiotics usually exist in the form of spores with strong stress resistance, and the types of the probiotics mainly comprise bacillus subtilis, bacillus licheniformis, bacillus coagulans and the like. In the use method, the product is orally taken, can be mixed into feed, and can also be added into drinking water, but the drinking water has more advantages than the mixing use. On one hand, when the probiotic is used by drinking water, the probiotic is in a liquid environment, and the space is further released after dilution, so that the recovery, amplification and metabolism of thalli are facilitated, and the using effect is better than that of mixing materials. On the other hand, with the acceleration of the large-scale and intensive feeding mode in China, the farm has become a trend of feeding with complete feed, the finished feed needs to be mixed for the second time when the feed is mixed, time and labor are wasted, and the particle size and the properties of the feed particles are damaged in the mixing process for the particle complete feed, so that the production is influenced. The product has less chance of being used by mixing materials and more farms selecting drinking water for use.

Probiotic class product has the problem of blockking up the drinking water pipeline circuit when drinking water and uses, especially raises the plant of small-size animal, like chicken, duck, pigeon etc. these waterline pipelines are thinner, and probiotic liquid blocks up the drinking water nipple very easily. Some pig farms can also form blockage when the water pressure is insufficient. The blockage is mainly caused by the fact that probiotic bacteria in the probiotic bacteria settle in water, gather at the bottom of a tube and move to a drinking nipple through the flowing of water. The blocked waterline can cause water break of animals due to unsmooth water flow, the animals are easy to suffer from heatstroke and die in hot summer if the water break time is too long, and even if the water break time is short, the animals can also suffer from water break stress to influence the production performance. The prior art also does not have efficient solution to this problem, treat to use up this type of product usually after, need in time to let in the pipeline with the copper wire of hairbrush and carry out mechanical cleaning, the reuse high-pressure squirt washes, nevertheless because the waterline pipeline is thinner, length is longer, and has many places turning and buckling again in the middle of, causes the clearance once to waste time and energy very much, has directly restricted probiotic class product in the clinical application of animal doctor, leads to its use value of unable full play of this type of product yet.

Disclosure of Invention

Aiming at the problem of water line blockage in clinical use of probiotic products, the invention aims to provide a preparation for preventing probiotic liquid from blocking a water line. The preparation is added into drinking water with probiotic bacteria liquid at a low concentration, stirred and dissolved, thallus components in the probiotic bacteria liquid can be suspended for a long time after being uniformly mixed, do not sink and stick to the wall, smoothly pass through a drinking nipple, and the problem that a water line is blocked when the probiotic bacteria product is used in drinking water is solved. Meanwhile, the invention is safe to use, does not block the waterline and does not influence the water quality and the effect of the probiotic product; the second purpose of the invention is to provide the preparation method for preventing the probiotic liquid from blocking the waterline preparation. The product has strong operability, is beneficial to production conversion and has wide market prospect.

In order to realize the purpose, the invention adopts the following technical scheme: a preparation for preventing probiotic liquid from blocking a water line comprises the following components in weight per 100g of the preparation: 1.0-5.0 g of azulene, 1.0-10.0 g of sesbania gum, 901.0-15.0 g of polyvinylpyrrolidone K, 0.1-2.0 g of calcium chloride, 1.0-6.0 g of propylene glycol alginate, 1.0-20.0 g of glyceryl triacetate, 0.1-2.0 g of aluminum stearate and the balance of deionized water.

Preferably, the formulation consists of, per 100g by weight: 1.5-4.5 g of azulene, 2.0-9.0 g of sesbania gum, 14.0g of polyvinylpyrrolidone K902.0, 0.5-1.5 g of calcium chloride, 1.5-5.5 g of propylene glycol alginate, 2.0-19.0 g of glyceryl triacetate, 0.3-1.8 g of aluminum stearate and the balance of deionized water.

More preferably, 3.0g of azulene, 5.5g of sesbania gum, 5.0g of polyvinylpyrrolidone K908.0 g, 1.0g of calcium chloride, 3.5g of alginic acid propylene glycol ester, 10.0g of triacetin, 1.0g of aluminum stearate and 68.0g of deionized water.

The preparation method of the preparation for preventing the probiotic liquid from blocking the water line comprises the following steps:

1) adding the azure, sesbania gum, polyvinylpyrrolidone k90 and calcium chloride into deionized water for dissolving to obtain a mixed solution A;

2) adding aluminum stearate into glyceryl triacetate, mixing, heating to 85-95 ℃, converting the turbid system into clear system, cooling to room temperature, and changing to turbid state to obtain a mixed solution B;

3) and adding the propylene glycol alginate into the mixed solution B, uniformly mixing, performing circulating high-speed grinding and dispersion by using a colloid mill, simultaneously adding the mixed solution A while grinding until the system is converted into uniform viscous liquid, and subpackaging.

The probiotic liquid product has reasonable formula, can effectively prevent the problem of water line blockage when the probiotic liquid product is clinically drunk, is safe to use, does not influence the water quality and the effect of the probiotic product, has strong process operability, and is beneficial to production conversion. Compared with the mechanical scouring in the prior art, the use method is simple and convenient, the manpower and material resources are obviously saved, and the market prospect is wider.

In the formula of the invention, the components have the following effects:

1) the selected azure gum and the selected azure gum are used as the components, because the fine colloidal particles formed by the azure gum and the selected azure gum have similar density with water, and have a certain adsorption effect on probiotics, the characteristics enable the probiotics to be suspended in water for a long time without precipitation, and prevent waterline nipples from being blocked;

2) polyvinylpyrrolidone k90 is selected as the component, because the polyvinylpyrrolidone k90 has a longer molecular chain, the sedimentation of suspended matters can be obviously slowed down after dissolution, and meanwhile, the viscosity of the aqueous solution is higher, so that the polyvinylpyrrolidone k90 has an auxiliary effect on the effect of preventing the probiotic liquid from blocking the waterline;

3) the calcium chloride is used as the component of the invention, because the calcium chloride can effectively help the liquid surface agglomeration phenomenon generated when the azure, sesbania gum and polyvinylpyrrolidone K90 are dissolved, and meanwhile, the calcium chloride is a heat release process when the calcium chloride is dissolved, the dissolution of the azure, sesbania gum and polyvinylpyrrolidone K90 is accelerated, and the production efficiency is improved. In addition, experiments show that the calcium chloride has a large maintaining effect on the system stability;

4) the glyceryl triacetate and the aluminum stearate are adopted as the components of the invention, and the combination of the glyceryl triacetate and the aluminum stearate can form a colloid with better quality, which is suitable for the invention, thus being beneficial to maintaining the stability of the product and enhancing the suspension of probiotics in water solution when drinking water;

5) propylene glycol alginate is used as the component of the invention because the mixed liquid A and the mixed liquid B in the step of the invention can be better emulsified together under the grinding and dispersing action of a colloid mill to form a stable system and ensure the product quality.

Besides the advantages of the formula, the process has strong operability, low requirements on production equipment and workshops, can be used for conversion production in most enterprises, is beneficial to popularization of products, and has a wider prospect.

Compared with the prior art, the invention achieves the technical effects that:

1) the use is convenient: when the cleaning agent is clinically used, only 0.01-0.5% of the mass concentration of the cleaning agent is added into drinking water, and probiotic liquid is added after the cleaning agent is uniformly dissolved, so that the blockage of a waterline can be effectively avoided, and compared with the mechanical cleaning method in the prior art, the cleaning agent is time-saving and labor-saving;

2) the use effect is obvious: the sedimentation and wall sticking of the probiotic bacteria can be effectively avoided, the bacteria are promoted to be suspended in the water solution for a long time and smoothly flow out through the drinking nipple, and the problem that the water line is blocked when the probiotic bacteria product is clinically drunk is solved;

3) the preparation process is simple: the preparation process of the invention has strong operability, is beneficial to production conversion and has good market prospect.

Detailed Description

The invention will be further illustrated by the following specific examples, which are not intended to limit the scope of the invention in any way.

Examples 1 to 10

For the sake of simplicity of the description, the weight composition of a preparation for preventing the clogging of the water line by probiotics described in examples 1-10 is given below in the form of a table, which is shown in table 1.

TABLE 1 compositions by weight per 100g of the formulations of the invention in examples 1-10

The method for preparing the preparation for preventing the probiotic liquid from blocking the water line according to the embodiments 1 to 10 comprises the following steps:

(1) adding the azure, sesbania gum, polyvinylpyrrolidone k90 and calcium chloride into deionized water for dissolving to obtain a mixed solution A;

(2) adding aluminum stearate into glyceryl triacetate, mixing, heating to 85-95 ℃, changing the turbid state of a system into clear state, and cooling to room temperature to obtain a mixed solution B;

(3) and adding the propylene glycol alginate into the mixed solution B, uniformly mixing, performing circulating high-speed grinding and dispersion by using a colloid mill, simultaneously adding the mixed solution A while grinding until the system is converted into uniform viscous liquid, and subpackaging.

Test examples 1 to 4:

test example 1 stability test of the product of the present invention

Stability is a basic guarantee of the product entering the market, examples 1-10 of the invention were sampled and subjected to an accelerated test (40 ℃), a long-term test (room temperature), a refrigerated test (4 ℃) and a freezing test (-20 ℃) respectively. After the sample is placed, the sample is sampled and observed at the time of 7d, 15d, 30d, 60d, 90d, 180d and 360d respectively, and the result shows that the appearance and the property of the product obtained in each example are not different from those before the test, and the product has good stability.

Test example 2 safety test

In order to better popularize the product in the market and prevent the problem of side effects in the using process, the safety of the invention is verified by clinical tests.

Healthy Hailan brown laying hen groups of 270 days old and having the weight of about 2kg in the egg producing period are selected, the size of the stockbreeding is 5000, 1000 groups are divided into 5 groups, wherein the 1 st group to the 4 th group are test groups, drinking water of one day all day is prepared into preparations (taking example 1) with the mass concentration of 0.01 percent, 0.05 percent, 0.1 percent and 0.5 percent for preventing the probiotic bacteria liquid from blocking the water line, the 5 th group is a blank control group, and no medicine is added into the drinking water. All the groups of chickens are bred in the same henhouse, all the environmental conditions are the same, the breeding management is carried out by the same breeder, the test period is one week, the feeding, drinking and mental states of all the groups of chickens are observed during the test period, and the first time is to perform the autopsy if the chickens die. After the test is finished, 5 chickens in each group are randomly selected to be subjected to a autopsy, and whether each organ has the pathological changes of the poisoning characteristic sample is judged, so that the safety of the invention is judged. In addition, the laying rates and average egg weights of all groups of chickens in the test period are counted and recorded to judge the influence of the invention on the production performance of the laying hens, and the result is shown in table 2.

The experimental results are as follows:

1) no death occurs in the test period of the test chickens, the ingestion, drinking, mental state and the like are normal, and no death occurs in the chickens. After the test is finished, 5 chickens in each group are randomly selected for performing the autopsy, and the parts of the liver, the kidney, the lung, the heart, the spleen, the intestinal tract, the trachea, the myoglandular stomach, the crop, the oral cavity, the oviduct and the like are observed, so that the results show that the groups 1-4 and the group 5 in the control group are normal and have no significant difference.

2) The results of the effect of the present invention on egg production performance are shown in table 2.

Table 2 results of the effect of the formulations of the invention on the egg laying performance of the hens in the laying period

As can be seen from Table 2: the preparation for preventing probiotic liquid from blocking the waterline does not influence indexes such as laying rate, egg weight and the like of the laying hens, and is safe in clinical use.

The results show that: the preparation for preventing probiotic liquid from blocking the waterline is safe to use clinically, is used according to the specified dosage, and does not bring side effect to the egg laying performance of poultry.

Experimental example 3 verification test of suspending effect

The preparation of the invention prepared in example 1 was prepared into four concentrations of 0.01%, 0.05%, 0.1% and 0.5% with drinking water, and placed in 100ml beakers No. 1, 2, 3 and 4, respectively, 90ml of each beaker was added, 10ml of commercially available enterococcus faecalis fermentation broth was added to each beaker and mixed well, and 90ml of water and 10ml of enterococcus faecalis fermentation broth were added to the beaker No. 5 as a control. And then, performing suspension observation at 1h, 2h, 4h, 8h, 12h, 24h, 36h and 48h respectively to see whether the bottom of the beaker has the probiotics precipitate or not, and performing test record. The results are shown in Table 3.

TABLE 3 suspending aid effect test results of enterococcus faecalis fermentation liquid according to the present invention

(Note: □ "indicates good suspension and uniform turbidity of the system;". smallcircle "indicates stratification without sediment at the bottom;". tangle-solidup "indicates apparent sediment at the bottom of the beaker)

And (4) analyzing results: the suspending effect is the basic effect of preventing the precipitation of the probiotic liquid, and the probiotics can be uniformly distributed in water for a long time only if the suspending effect is good. According to the results in table 3, the lowest concentration cup No. 1 showed stratification at 12h and precipitation at 24h, and as the dosage concentration of the present invention increased, cup No. 2 showed stratification at 24h, precipitation at 36h, stratification at 36h, precipitation at 48h, stratification at 36h for cup No. 3, and no precipitation at the end of the test was observed until the end of the test. As a control group 5, in the case where the present invention was not used, delamination occurred at the 4 th hour and precipitation occurred at the 8 th hour. For practical clinical application, when drinking water, the probiotic liquid is usually drunk in a chicken house, namely the probiotic liquid is required to be drunk up within 4 hours, so that the probiotic liquid is required not to be layered or precipitated within 4 hours, obviously, the No. 5 cup does not meet the requirement, and the No. 1-4 cups can meet the clinical use requirement.

The results show that: the preparation for preventing probiotic liquid from blocking the waterline has a good suspending effect.

Test example 4 clinical Effect test

Selecting 15000 egg chicken farms with the size of a storage column, dividing the egg chicken farms into 3 groups according to 5000 groups, taking the group 1 as the test group of the invention, taking the sample prepared in the embodiment 1 as an example, adding the preparation of the invention into drinking water according to the mass concentration ratio of 0.1%, stirring and dissolving, then adding the commercial enterococcus faecalis fermentation liquor according to the mass concentration ratio of 0.2%, and uniformly stirring and then supplying the liquor for chicken flocks to drink. The group 2 is a probiotic bacteria liquid group, commercial enterococcus faecalis fermentation liquid is added into drinking water according to the mass concentration ratio of 0.2% for chicken to drink, and the group three is a blank control group which only drinks tap water. All the groups of chickens are raised in the same environment and are raised by the same breeder, the test period is 5d, the medicine is applied for 1 time every day according to the operation method of the first day, and the water flow condition of drinking nipples in the morning and afternoon is checked and recorded. The results are shown in Table 4.

TABLE 4 results of clinical efficacy test of the formulations of the present invention

(Note: ". smallcircle" indicates that the water flow is normal and the drinking nipple is not blocked,

the water flow is reduced, the drinking nipple is not completely blocked, the ● water flow disappears, the drinking nipple is completely blocked

And (3) test results:

group 1, the preparation prepared in the embodiment 1 of the invention is dissolved, and then enterococcus faecalis fermentation liquor is added until the 5d test is finished, and the waterline is not blocked;

in the group 2, water is not treated, enterococcus faecalis fermentation liquor is directly added, and the result shows that the water flow of the drinking nipple is reduced in the 3 rd morning, which indicates that a half-blockage situation occurs, and the drinking nipple is completely blocked in the 4 th afternoon, so that the water flow disappears;

the group 3 is a control group, only tap water is drunk in the whole process, and the water line is not blocked.

The results show that: the preparation for preventing probiotic liquid from blocking the waterline can effectively prevent the probiotic liquid from blocking the waterline.

Claims (4)

1. A preparation for preventing probiotic liquid from blocking a water line is characterized by comprising the following components in weight per 100 g: 1.0-5.0 g of azulene, 1.0-10.0 g of sesbania gum, 901.0-15.0 g of polyvinylpyrrolidone K, 0.1-2.0 g of calcium chloride, 1.0-6.0 g of propylene glycol alginate, 1.0-20.0 g of glyceryl triacetate, 0.1-2.0 g of aluminum stearate and the balance of deionized water.

2. The preparation for preventing probiotic bacteria from blocking waterlines of claim 1, wherein the weight per 100g of the preparation comprises: 1.5-4.5 g of azulene, 2.0-9.0 g of sesbania gum, 14.0g of polyvinylpyrrolidone K902.0, 0.5-1.5 g of calcium chloride, 1.5-5.5 g of propylene glycol alginate, 2.0-19.0 g of glyceryl triacetate, 0.3-1.8 g of aluminum stearate and the balance of deionized water.

3. The preparation for preventing probiotic bacteria from blocking waterlines of claim 2, wherein the weight per 100g of the preparation is comprised of: 3.0g of azulene, 5.5g of sesbania gum, K908.0 g of polyvinylpyrrolidone, 1.0g of calcium chloride, 3.5g of propylene glycol alginate, 10.0g of glyceryl triacetate, 1.0g of aluminum stearate and 68.0g of deionized water.

4. The method for preparing the preparation for preventing the probiotic liquid from blocking the water line according to claim 1, 2 or 3, characterized by comprising the following steps:

1) adding the azure, sesbania gum, polyvinylpyrrolidone k90 and calcium chloride into deionized water for dissolving to obtain a mixed solution A;

2) adding aluminum stearate into glyceryl triacetate, mixing, heating to 85-95 ℃, converting the turbid system into clear system, cooling to room temperature, and changing to turbid state to obtain a mixed solution B;

3) and adding the propylene glycol alginate into the mixed solution B, uniformly mixing, performing circulating high-speed grinding and dispersion by using a colloid mill, simultaneously adding the mixed solution A while grinding until the system is converted into uniform viscous liquid, and subpackaging.