CN113832031A

CN113832031A - Full-automatic cell preparation all-in-one

Info

Publication number: CN113832031A
Application number: CN202111134554.7A
Authority: CN
Inventors: 周志勇; 赵国闯; 李超
Original assignee: Suzhou Xinxie Biotechnology Co ltd
Current assignee: Suzhou Xinxie Biotechnology Co ltd
Priority date: 2021-09-27
Filing date: 2021-09-27
Publication date: 2021-12-24

Abstract

The invention discloses a full-automatic cell preparation all-in-one machine, which comprises a machine cabinet, a magnetic separation module and a centrifugal module, wherein the machine cabinet comprises an upper cabinet body, a lower cabinet body and a workbench, the workbench is provided with an installation platform, the installation platform is also provided with a pipeline system, a plurality of pinch valves, a plurality of flow sensors, the magnetic separation module, a cultivation module, a peristaltic pump, a counting module, the centrifugal module and a controller, the magnetic separation module comprises a bearing platform and an electromagnet, the bearing platform is fixed on the installation platform, one end of the bearing platform is provided with a groove, two tube clamping seats are arranged in the groove, branch pipelines are clamped on the tube clamping seats, the other end of the bearing platform is provided with a magnet fixing plate, the electromagnet is arranged on the magnet fixing plate, two side walls of the electromagnet are respectively positioned at two sides of the groove, and two tube clamping grooves which are oppositely arranged are arranged on the tube clamping seats; the invention can realize high integration and full automation, and ensure the stability and uniformity of cell quality and the repeatability of experimental results.

Description

Full-automatic cell preparation all-in-one

Technical Field

The invention relates to the technical field of cell preparation, in particular to a full-automatic cell preparation all-in-one machine.

Background

Cell preparation refers to a process in which scientific research technicians use a relatively mature cell culture scheme to prepare a high-purity target cell final product which can be directly applied to the scientific research and clinical fields after function and quality detection. In the processes of cell preparation and cell culture, the prepared or cultured cells must be subjected to various detections including detection of various exogenous factors, check of cell morphology, analysis of cell number, cell activity, cell phenotype and the like, which represent the cell preparation or culture quality, so as to ensure that the cells are effective cells with no pollution, high purity and high activity in the processes of preparation and culture and final collection and utilization.

Traditional cell preparation mode adopts blake bottle or culture dish to carry out manual culture for through scientific research personnel in clean laboratory, in order to cultivate a large amount of cells, needs to have the technical staff of high level operation, spends a large amount of time, still needs a large amount of blake bottles and culture space, cultivates inefficiency, can't realize large-scale production. Although there are some automated cell preparation devices on the market, there is a major drawback in these devices: the separation and the culture are both carried out in a separation culture tank, the requirements of the separation and the culture on the volume cannot be considered, so that the culture volume is greatly limited, for example, the maximum culture volume of a cell preparation system in the Tian and whirlwind series of Germany and America is only 250ml, and the preparation efficiency of cells is greatly reduced. In view of the above disadvantages, it is necessary to design a fully automatic cell preparation machine.

Disclosure of Invention

The invention aims to provide a full-automatic cell preparation all-in-one machine to solve the problems in the background technology.

In order to achieve the purpose, the invention provides the following technical scheme: a full-automatic cell preparation all-in-one machine comprises a machine cabinet, a magnetic separation module and a centrifugal module, wherein the machine cabinet comprises an upper cabinet body, a lower cabinet body and a workbench, a support is arranged in the machine cabinet, a touch display screen is arranged on the support, a plurality of medium bag supports are arranged at the top of an installation platform, medium bags are hung on the medium bag supports, a pipeline system, a plurality of pinch valves, a plurality of flow sensors, the magnetic separation module, a cultivation module, a peristaltic pump, a counting module, the centrifugal module and a controller are further arranged on the installation platform, the magnetic separation module comprises a bearing platform and an electromagnet, the bearing platform is fixed on the installation platform, a groove is formed in one end of the bearing platform, two clamping tube seats are arranged in the groove, branch pipelines are clamped on the clamping tube seats, a magnet fixing plate is arranged at the other end of the bearing platform, and the electromagnet is arranged on the magnet fixing plate, the electromagnet is of a U-shaped structure, and two side walls of the electromagnet are respectively positioned on two sides of the groove. The pipe clamping seat is provided with two opposite pipe clamping grooves, each pipe clamping groove is of a semi-tubular structure, and a longitudinal circular tubular cavity is formed between the two pipe clamping grooves.

Preferably, the workbench is located between the upper cabinet body and the lower cabinet body, a single-opening cabinet door is arranged on the upper cabinet body, an installation platform is arranged on the workbench, and the indicator lamp is installed at the top of the cabinet.

Preferably, the pipeline system is respectively communicated with the medium bag, the magnetic separation module, the cultivation module, the peristaltic pump, the counting module and the centrifugal module through a plurality of branch pipelines, and the cultivation module comprises a cultivation cabin and a cultivation container.

Preferably, the centrifugal module comprises a centrifugal driving assembly and a centrifugal cabin assembly which are matched with each other, the centrifugal driving assembly is used for carrying out high-speed density gradient centrifugal separation on a medium to be centrifuged to obtain a required component, and the centrifugal cabin assembly is used for providing a closed isolation place for centrifugal operation of the centrifugal driving assembly and providing various optical devices and sensors for monitoring.

Preferably, the centrifugal driving assembly comprises a disc bottom plate fixedly connected with the centrifugal cabin assembly, the centrifugal cabin assembly comprises a centrifugal cabin and a centrifugal cover which are matched with each other, the centrifugal cover is covered on the centrifugal cabin, support legs are installed at the bottom of the disc bottom plate and arranged in the mounting table, and a motor fixing frame is further fixedly installed on the disc bottom plate.

Preferably, install centrifugal motor on the motor mount, driving pulley is installed to centrifugal motor's output, and driving pulley passes through the drive belt and is connected with from the driven pulleys, and from the driven pulleys installs the one end at the transmission shaft, and the transmission shaft passes through the bearing and installs on the disc bottom plate, the other end fixedly connected with sample jar of transmission shaft, sample jar are located in the centrifugal chamber subassembly.

Preferably, the motor fixing frame is further provided with a tensioning wheel, and the tensioning wheel is located between the driving pulley and the driven pulley and is in contact with the transmission belt to tension the transmission belt.

Compared with the prior art, the full-automatic cell preparation all-in-one machine can well sort cells with magnetic beads through the matching between the arranged bearing platform and the electromagnet, can enable the cells with the magnetic beads to be adsorbed on the branch pipelines, and can quickly install and fix the branch pipelines in the two clamping pipe grooves through the arrangement of the clamping pipe seats, so that the cell preparation efficiency is improved; the full-automatic cell preparation system provided by the invention has the advantages that the medium bag, the centrifugal module, the culture module, the counting module and the magnetic sorting module are sequentially communicated through the pipeline system, and the conveying, recovery and accurate control of various media are realized by matching with the use of devices such as a controller, a peristaltic pump, a pinch valve, a flow sensor and the like, so that high integration and full automation are realized, full-automatic production can ensure that each batch of cells are prepared under the same standard condition, the stability and uniformity of cell quality and the repeatability of experimental results are ensured, and the full-automatic cell preparation system has the condition for realizing large-scale production; the invention separates the centrifugal station from the culture station, the centrifugation module completes the centrifugation of cells, the culture module completes the culture of cells, and the independent operation can meet the requirements of the separation and the culture on the volume respectively; the culture module is internally provided with the cell culture container with adjustable volume, so that the volume can be adjusted or a cell culture bag with proper volume can be selected according to actual needs without the limitation of a centrifugal module, and the preparation efficiency of cells is greatly improved; the tension pulley is arranged, so that the tension degree of the transmission belt can be conveniently adjusted, the running stability of the centrifugal driving assembly is ensured, and the stability of cell quality is ensured.

Drawings

The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the principles of the invention without limiting the invention in which:

FIG. 1 is a schematic structural diagram of a fully automatic cell preparation machine according to the present invention;

FIG. 2 is a schematic structural view of a fully automatic integrated cell preparation machine of the present invention without an upper cabinet and a single door;

FIG. 3 is a schematic structural diagram of an installation table in a fully automatic cell preparation all-in-one machine according to the present invention;

FIG. 4 is a schematic structural diagram of a magnetic sorting module in a fully automatic integrated cell preparation machine according to the present invention;

FIG. 5 is a schematic diagram of a centrifugal module in an integrated automatic cell preparation machine according to the present invention.

In the drawings:

1. a cabinet; 2. an upper cabinet body; 3. a lower cabinet body; 4. a work table; 5. a single-door opening cabinet door; 6. an installation table; 7. an indicator light; 8. a support; 9. a touch display screen; 10. a media bag holder; 11. a pinch valve; 12. a magnetic sorting module; 13. a cultivation module; 14. a peristaltic pump; 15. a centrifuge module; 16. a cultivation cabin; 17. a receiving table; 18. an electromagnet; 19. a groove; 20. a cartridge holder; 21. a magnet fixing plate; 22. a centrifugal drive assembly; 23. a centrifuge capsule assembly; 24. a disc base plate; 25. a support leg; 26. a motor fixing frame; 27. a centrifugal motor; 28. a driving pulley; 29. a transmission belt; 30. a driven pulley; 31. a drive shaft; 32. a bearing; 33. a sample tank; 34. a tension wheel; 35. a centrifugal chamber; 36. a centrifuge cap; 37. a pipe clamping groove.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Referring to fig. 1-5, the present invention provides a technical solution: the utility model provides a full-automatic cell preparation all-in-one, includes rack 1, magnetism sorting module 12 and centrifugal module 15, rack 1 includes the last cabinet body 2, lower cabinet body 3 and workstation 4, workstation 4 is located between the last cabinet body 2 and the lower cabinet body 3, be provided with single-open cabinet door 5 on the last cabinet body 3, be provided with mount table 6 on the workstation 4, pilot lamp 7 is installed at the top of rack 1, rack 1 internally mounted has support 8, installs touch display screen 9 on the support 8, a plurality of medium bag supports 10 are installed at the top of mount table 6, hang on a plurality of medium bag supports 10 and be equipped with the medium bag, still install pipe-line system, a plurality of pinch valves 11, a plurality of flow sensor, magnetism sorting module 12, cultivate module 13, peristaltic pump 14, count module, centrifugal module 15 and controller on the mount table 6, wherein, pipe-line system communicates medium bag respectively through many branch pipelines, The magnetic sorting module 12, the cultivating module 13, the peristaltic pump 14, the counting module and the centrifugal module 15 realize the conveying, recovery and accurate control of various components; the pinch valves 11, the flow sensors, the touch display screen 9, the magnetic sorting module 12, the cultivating module 13, the peristaltic pump 14, the counting module and the centrifugal module 15 are respectively connected to a controller, so that interaction and control of all the components are realized.

In this embodiment, the controller is a PLC controller, and a logic instruction output program of each module, which is pre-designed according to the full-automatic cell culture process, is provided in the PLC controller, so as to implement automatic operation of the whole cell preparation system.

In this embodiment, the medium bag is used for storing blood or tissue fluid, separation fluid, physiological saline, and the like, and can also be used for storing or transferring various types of fluids output in the process engineering; the peristaltic pump 14 acts under the action of the controller to provide driving force for the medium in the pipeline system; the centrifugal module 15 is used for automatically separating, extracting and transferring the medium to be centrifuged transferred from the medium bag into the medium bag after high-speed density gradient centrifugation, and then selecting the required components to be conveyed to the cultivation module 13; the culture module 13 is internally provided with a cell culture container with adjustable volume, and is used for culturing target cells obtained after centrifugation; the magnetic sorting module 12 is used for sorting the target cells and sorting out non-target cells; the counting module is used for counting cells and observing morphology; the flow sensor is used for monitoring the flow in each pipeline and transmitting a signal to the controller.

In this embodiment, the incubation module 13 includes an incubation compartment 16, which houses a volume-adjustable cell culture container, and an incubation container, which facilitates adjustment of the volume of the incubation container.

In this embodiment, the pinch valves 11 are electromagnetic valves and can receive an instruction action of a controller, each electromagnetic valve automatically controls the on-off of one pipeline, so as to realize automatic control, and the specific connection mode and position of the pinch valves 11 are adjusted and designed according to actual needs, so as to provide power for the medium flow in the pipeline of each pipe in the pipeline system in this embodiment.

In this embodiment, module 12 is selected separately to magnetism includes bearing platform 17 and electro-magnet 18, bearing platform 17 is fixed on mount table 6, recess 19 has been seted up to bearing platform 17's one end, and recess 19 internally mounted has two tube seat 20, and the joint has the branch pipeline on the tube seat 20, bearing platform 17's the other end is provided with magnet fixed plate 21, installs electro-magnet 18 on the magnet fixed plate 21, electro-magnet 18 is the U-shaped structure, the both sides wall of electro-magnet 18 is located the both sides of recess 19 respectively. The utility model discloses a cell preparation method, including the step of the utility model discloses a cell preparation method, be equipped with the card pipe seat 20 and be equipped with two relative card pipe grooves 37 that set up, card pipe groove 37 is half tubular structure, forms fore-and-aft pipe form cavity between two card pipe grooves 37, can install fast fixedly the branch pipeline in two card pipe grooves 37 to the preparation efficiency of cell has been improved.

In this embodiment, the centrifuge module 15 includes a centrifugal drive assembly 22 and a centrifuge capsule assembly 23 that cooperate with each other, the centrifugal drive assembly 22 is used for high-speed density gradient centrifugation of the medium to be centrifuged to separate the desired components, and the centrifuge capsule assembly 23 is used for providing a closed isolation space for the centrifugation of the centrifugal drive assembly 22 and various types of optical devices and sensors for monitoring.

In this embodiment, the centrifugal drive assembly 22 comprises a disc bottom plate 24 fixedly connected to the centrifuge capsule assembly 23, the centrifuge capsule assembly 23 comprises a centrifuge capsule 35 and a centrifuge cover 36, which are fitted to each other, the centrifuge cover 36 being provided over the centrifuge capsule 35, the bottom of the disc bottom plate 24 is provided with a support leg 25, the support leg 25 is arranged in the mounting table 6, a motor fixing frame 26 is also fixedly arranged on the disc bottom plate 24, a centrifugal motor 27 is arranged on the motor fixing frame 26, a driving belt wheel 28 is arranged at the output end of the centrifugal motor 27, the driving belt wheel 28 is connected with a driven belt wheel 30 through a transmission belt 29, the driven belt wheel 30 is arranged at one end of a transmission shaft 31, the transmission shaft 31 is arranged on the disc bottom plate 24 through a bearing 32, the other end of the transmission shaft 31 is fixedly connected with a sample tank 33, the sample tank 33 is positioned in the centrifugal chamber assembly 23, and the medium to be centrifuged is subjected to high-speed density gradient centrifugation in the sample tank 33.

In this embodiment, the motor fixing frame 26 is further provided with a tension pulley 34, and the tension pulley 34 is located between the driving pulley 28 and the driven pulley 30 and contacts with the transmission belt 29 to tension the transmission belt 29. By arranging the tension wheel 34, the tension degree of the transmission belt 29 can be conveniently adjusted, and the running stability of the centrifugal driving assembly 22 is ensured, so that the stability of cell quality is ensured.

When the invention is used, a power supply is started, the volume of a cell culture container in the culture cabin 16 is automatically or manually adjusted, an automatic program is started through the operation of the touch display screen 9, a medium bag is hung on the medium bag support 10, the medium bag enables blood or tissue fluid to enter a sample tank 33 of the centrifugal module 15 through a pipeline system according to the control parameters of the set program such as timing, quantification, constant speed and the like to perform high-speed density gradient centrifugation, the blood or tissue fluid is automatically separated, extracted and transferred into the medium bag through the set program and image algorithm, waste liquid in the sample tank 33 is discharged, then the blood or tissue fluid is transferred back to the sample tank 33 of the centrifugal module 15 to perform magnetic bead incubation, the incubated liquid is transferred to the culture cabin 16 of the culture module 13 through the medium bag after the incubation is finished, more specifically, the cell culture container with adjustable volume in the culture cabin 16 is cultured, and automatic counting and continuous monitoring are realized through the counting module, then the concentrated mixed liquid in the branch pipeline passes through the electromagnet 18, so that the cells with magnetic beads are adsorbed on the pipe wall of the branch pipeline, the rest cell liquid flows into the lower part and is sent to the culture cabin 16 of the culture module 13, the target cells are finally obtained, the liquid transfer in the whole process is powered by the peristaltic pump 14, and the cell preparation is completed.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. The utility model provides a full-automatic cell preparation all-in-one which characterized in that: the magnetic separation device comprises a cabinet (1), a magnetic separation module (12) and a centrifugal module (15), wherein the cabinet (1) comprises an upper cabinet body (2), a lower cabinet body (3) and a workbench (4), a support (8) is installed inside the cabinet (1), a touch display screen (9) is installed on the support (8), an installation table (6) is arranged on the workbench (4), a plurality of medium bag supports (10) are installed at the top of the installation table (6), medium bags are hung on the medium bag supports (10), a pipeline system, a plurality of pinch valves (11), a plurality of flow sensors, the magnetic separation module (12), a cultivation module (13), a peristaltic pump (14), a counting module, the centrifugal module (15) and a controller are further installed on the installation table (6), the magnetic separation module (12) comprises a bearing table (17) and an electromagnet (18), and the bearing table (17) is fixed on the installation table (6), one end of bearing platform (17) is seted up flutedly (19), and recess (19) internally mounted has two chuck tube seats (20), and the joint has the branch pipeline on chuck tube seat (20), the other end of bearing platform (17) is provided with magnet fixed plate (21), installs electro-magnet (18) on magnet fixed plate (21), electro-magnet (18) are the U-shaped structure, the both sides wall of electro-magnet (18) is located the both sides of recess (19) respectively, be equipped with chuck tube groove (37) of two relative settings on chuck tube seat (20), chuck tube groove (37) are half tubular structure, form fore-and-aft circle tubulose cavity between two chuck tube groove (37).

2. The automatic cell preparation all-in-one machine of claim 1, wherein: the working table (4) is located between the upper cabinet body (2) and the lower cabinet body (3), a single-opening cabinet door (5) is arranged on the upper cabinet body (3), and an indicator lamp (7) is installed at the top of the cabinet (1).

3. The automatic cell preparation all-in-one machine of claim 1, wherein: the pipeline system is respectively communicated with the medium bag, the magnetic sorting module (12), the cultivation module (13), the peristaltic pump (14), the counting module and the centrifugal module (15) through a plurality of branch pipelines, and the cultivation module (13) comprises a cultivation cabin (16) and a cultivation container.

4. The automatic cell preparation all-in-one machine of claim 1, wherein: the centrifugal module (15) comprises a centrifugal driving assembly (22) and a centrifugal cabin assembly (23) which are matched with each other, the centrifugal driving assembly (22) is used for carrying out high-speed density gradient centrifugal separation on a medium to be centrifuged to obtain required components, and the centrifugal cabin assembly (23) is used for providing a closed isolation place for centrifugal operation of the centrifugal driving assembly (22) and providing various optical devices and sensors for monitoring.

5. The automatic cell preparation all-in-one machine of claim 4, wherein: centrifugal drive subassembly (22) include with centrifugation cabin subassembly (23) fixed connection's disc bottom plate (24), centrifugation cabin subassembly (23) are including centrifugation cabin (35) and centrifugal cover (36) of mutually supporting, centrifugal cover (36) lid is established on centrifugation cabin (35), stabilizer blade (25) are installed to disc bottom plate (24) bottom, and stabilizer blade (25) set up in mount table (6), still fixed mounting has motor mount (26) on disc bottom plate (24).

6. The automatic cell preparation all-in-one machine of claim 5, wherein: install centrifugal motor (27) on motor mount (26), driving pulley (28) are installed to the output of centrifugal motor (27), and driving pulley (28) pass through drive belt (29) and are connected with driven pulley (30), and the one end at transmission shaft (31) is installed in driven pulley (30), and install on disc bottom plate (24) transmission shaft (31) through bearing (32), the other end fixedly connected with sample tank (33) of transmission shaft (31), sample tank (33) are located in centrifugal chamber subassembly (23).

7. The automatic cell preparation all-in-one machine of claim 6, wherein: the motor fixing frame (26) is also provided with a tension wheel (34), and the tension wheel (34) is positioned between the driving belt wheel (28) and the driven belt wheel (30) and is in contact with the transmission belt (29) to tension the transmission belt (29).