CN113832003A - Equipment convenient for extracting and preparing adipose-derived stem cell exosomes and extraction process - Google Patents
Equipment convenient for extracting and preparing adipose-derived stem cell exosomes and extraction process Download PDFInfo
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Abstract
The invention relates to the technical field of cell preparation, and provides equipment and an extraction process for conveniently extracting and preparing adipose-derived stem cell exosomes, which comprises a separation adjusting frame, an extraction head and a separation frame, wherein the separation adjusting frame comprises a pushing block, two sides of the pushing block are movably connected with rotating wheels for adjusting descending positions, one side of the pushing block, far away from the rotating wheels, is movably connected with a central rotating rod, two sides of the central rotating rod are respectively provided with a sliding connecting rod for adjusting pressure, the equipment and the extraction process for conveniently extracting and preparing the adipose-derived stem cell exosomes are characterized in that collected materials are pressed into an air bag frame through accumulation, so that the air bag frame drives a clamping plate frame and a stabilizing frame to be tightly buckled in a groove of the separation rotating block through an air column, the rotating stabilizing force in the air bag frame is increased, the separated sediment is the adipose-derived stem cell exosomes, and the stability of key matter-liquid separation is improved, the collection efficiency of the adipose-derived stem cell exosomes is improved.
Description
Technical Field
The invention relates to the technical field of cell preparation, in particular to equipment and an extraction process for conveniently extracting and preparing an adipose-derived stem cell exosome.
Background
The mesenchymal stem cell is one of important seed cells, has self-renewal and multidirectional differentiation potential, has stable genetic information, and can be extracted in large quantity by a simpler method. Compared with other mesenchymal stem cell sources, adipose tissues are tissues which are rich in sources, can be consumed and are easily obtained, so that the adipose mesenchymal stem cells gradually become a new favorite for stem cell research, and the research on the adipose mesenchymal stem cells is more and more. However, the storage condition of the adipose-derived mesenchymal stem cells is harsh, the transportation is inconvenient, and the clinical application is difficult.
The exosome is a cystic vesicle which is generated through an endosome pathway and released outside cells, is coated by a phospholipid double-layer membrane, and is internally coated with a plurality of bioactive substances such as lipid, protein, nucleic acid and the like, is cupped under the observation of an electron microscope, and plays an important role in the transfer of the substances among the cells and the information transmission. Recent studies have shown that exosomes play an important role in intercellular signal communication, have the functions of immunoregulation, promotion of angiogenesis, mediation of cell proliferation, differentiation, migration, apoptosis and the like, maintain the normal physiological state of the body and participate in disease processes. The mesenchymal stem cell-derived exosome has the characteristics of derived cells, and can promote self-repair and tissue regeneration of cells in a damaged area, restore tissue homeostasis, accelerate wound repair and the like. The exosome secreted by the human adipose-derived mesenchymal stem cell has a stable structure, is not easy to decompose, provides a new possibility for clinical application of the adipose-derived mesenchymal stem cell, and is released from the cell by a low-temperature centrifugation method from a cell culture medium in the prior extraction of the exosome.
However, the existing extraction equipment for collecting exosomes by using a centrifugal method is complex in operation, so that the extraction time is long, the extraction quantity is small, the overall extraction efficiency is low, in the extraction process, the exosomes are difficult to store and separate due to the instability of the suction force inside the equipment and the centrifugal force inside the equipment, and vesicles are easy to break due to high-speed centrifugation, so that the equipment and the extraction process convenient for extracting and preparing the adipose-derived stem cell exosomes are provided.
Disclosure of Invention
In order to solve the technical problems, the invention provides equipment and an extraction process for conveniently extracting and preparing adipose-derived stem cell exosomes, which are achieved by the following specific technical means:
the utility model provides an equipment convenient to extract preparation adipose-derived stem cell exosome, includes the separation adjustment frame, draws the head, the separation frame, the separation adjustment frame includes promotes the piece, the both sides swing joint that promotes the piece has the runner that is used for adjusting the decline position, one side swing joint that promotes the piece and keeps away from the runner has central bull stick, the both sides of central bull stick all are provided with the sliding connection pole that is used for adjusting pressure, one side of promoting the piece is provided with the waste liquid collecting pipe of invariable atmospheric pressure, it includes the pressure runner to draw the head, the outside swing joint of pressure runner has the slide bar that is used for flexible, the slide bar sets up the bottom port department at the waste liquid collecting pipe, the top swing joint of pressure runner has the push rod, the trachea frame that is used for collecting the cell is seted up to the inboard of push rod, the separation adjustment frame sets up the top of drawing the head, the separation frame includes spacing collection frame, the outside fixedly connected with screens board of frame is collected to spacing, one side that screens board was kept away from to spacing collection frame is provided with the separation turning block, the separation frame sets up the one side at the separation adjustment frame, screens board joint is in one side of waste liquid collecting pipe.
Through adopting above-mentioned scheme, the cooperation of pressure runner and slide bar increases inside pressure, has improved the stability that mixed liquid was collected, collects more exosomes, but has slowed down the speed of collecting of mixed liquid, and through the cooperation of separation turning block with spacing collection frame, avoids the high-speed centrifugation method of low temperature to obtain more exosomes collecting the vesicle breakage of exosome in-process and using shorter time.
Preferably, the outer side of the separation adjusting frame is movably connected with an equipment outer frame, one side of the equipment outer frame, which is far away from the separation adjusting frame, is movably connected with a clutch separation block for collecting cells, and the outer side of the separation frame is movably connected with a pressurizing stabilizing frame for dividing cells for storage.
By adopting the scheme, the structure linkage of the whole equipment is reinforced, and the disassembly is easy.
Preferably, the pressure boost steady rest includes the gas post, the both sides swing joint of gas post has the steady rest that is used for the expansion, the outside swing joint of steady rest has the cardboard frame, the top swing joint of gas post has the receipt gas pole, it is the encapsulated situation setting with the gas post to receive the gas pole, the gas post sets up the top port at spacing collection frame.
By adopting the above scheme, through the cooperation of gas post and cardboard frame, increase cardboard frame is to the suitability of separation turning block, has improved the stationarity when separation turning block is rotatory.
Preferably, the equipment outer frame comprises a placement frame, the top end of the placement frame is movably connected with a push rod used for controlling the sampling amount, and the push rod is arranged at the top end of the separation rotating block.
By adopting the scheme, the integral operation is simpler.
Preferably, the clutch separation block comprises an air bag frame, a sliding rail used for controlling pressure is arranged on the outer side of the air bag frame, the sliding rail is arranged on the outer side of the air column, and the sliding rail and the air column are arranged in a sealing state.
By adopting the scheme, the mixed liquid in each different stage is separated through the multi-layer air bag frame, so that the post treatment is facilitated.
Preferably, the number of the air bag frames is multiple groups, the air bag frames are arranged on the surface of the air receiving rod in a linear array mode, circulation pipes are arranged at ports of the upper side and the lower side of the air bag frames, and the circulation pipes of the air bag frames are in a circulation state when the air bag frames are pushed by the squeezed air pressure.
By adopting the scheme, the mixed liquid is simpler to store.
Preferably, the quantity of pressure runner is the multiunit, the multiunit the pressure runner is linear array and sets up on the surface of slide bar, the top port department swing joint of extrusion piece has the rubber sleeve, the rubber sleeve of extrusion piece is the state setting of cup jointing with the push rod.
By adopting the scheme, the transmission of each stage of the mixed liquid is ensured, and the volume of the mixed liquid obtained each time is fixed.
Preferably, the lateral cross-sectional area of separation turning block is less than the lateral cross-sectional area of spacing collection frame, the side of spacing collection frame and the outside looks adaptation of separation turning block, the cardboard frame sets up the both sides at the separation turning block.
By adopting the scheme, the stability of the separation turning block in rotation is improved.
An extraction process convenient for extracting and preparing adipose-derived stem cell exosomes comprises the following steps:
s1: suction of the mixed solution
At first through the pulling catch bar, suction through equipment inside adsorbs mixed liquid through drawing the head, and simultaneously the imbibition liquid drives the compression of pressure runner through inside suction, and the pressure runner is continuously compressed through the slide bar, because multiunit pressure runner is compressed, its inside pressure increases gradually, cause the rising speed of mixed liquid slower, and comparatively steady, air pressure drives the push rod through extrusion pressing block and extrudees to the outside, and the trachea frame is inside to be extruded gradually to inside air disappearance, move to the bottom of trachea frame until the push rod, then mixed liquid single absorption is accomplished, and through the inside gas volume restriction of trachea frame, the mixed liquid volume of representative absorption is unanimous.
S2: first Clutch step
And then the adsorbed mixed liquid is subjected to first clutch through circulation pipes of a first layer of air bag frame and a second layer of air bag frame at the bottom, the clutch force is 380 plus 420Xg, the separation rotating block is driven to rotate through driving, the redundant cell liquid is separated to the limiting collection frame through the clutch force, the limiting collection frame passes through small holes in the limiting collection frame, and the mixed liquid is transmitted to the next layer through the supporting pressing block.
S3: second Clutch step
The circulation pipe of the third layer of air bag frame and the fourth layer of air bag frame carries out the second separation and reunion to the mixed liquid on the absorption, the separation and reunion power is 1800 plus 2200xg, the unnecessary cellular fluid is separated to the spacing collection frame with the separation and reunion power on the same reason, spacing collection frame is through inside aperture, conduct unnecessary cellular fluid to spacing collection frame department, the mixed liquid conducts to next floor through supporting the briquetting simultaneously, push down the promotion piece down after that, it deflects to drive the inboard on central bull stick to promote the piece through the runner drive slip connecting rod, drive spacing collection frame and move to the outside simultaneously, through the extrusion, cause the unnecessary clout of separation in the spacing collection frame inside to store in waste liquid collecting pipe department.
S4: third Clutch step
And finally, carrying out second clutch on the adsorbed mixed liquid by the circulation pipes of the fifth layer air bag frame and the sixth layer air bag frame, wherein the clutch force is 100000-140000Xg, the centrifugal force in the fifth layer air bag frame is far greater than that in the first two times, the collected materials are stacked and extruded into the air bag frame, so that the air bag frame drives the clamping plate frame and the stabilizing frame to be tightly buckled in the groove of the separation rotating block through the air column, the rotating steady force in the air bag frame is increased, and the separated sediment is the adipose-derived stem cell exosome.
The invention has the following beneficial effects:
1. this equipment convenient to draw preparation adipose-derived stem cell exosome and extraction technology, drive the push rod through extrusion piece through air pressure and extrude to the outside, and the trachea frame is inside to be extruded gradually and disappear to inside air, move to the bottom of trachea frame until the push rod, then mixed liquid single absorbs and accomplishes, and through the inside gas volume restriction of trachea frame, the mixed liquid volume of representative absorption is unanimous, thereby realized that the quantity of drawing is comparatively average, and holistic extraction efficiency is higher, avoid extracting the inside cell of in-process and receive the extrusion and damage.
2. This equipment and extraction process convenient to draw preparation adipose stem cell exosome, separate unnecessary cell sap to spacing collection frame through separation and reunion power on, spacing collection frame is through inside aperture, conduct unnecessary cell sap to spacing collection frame department, mixed liquid conducts to next floor through supporting the briquetting simultaneously, push down the promotion piece after that, it drives the inboard deflection of slip connecting rod on central rotating rod through the runner to promote the piece, drive spacing collection frame outside simultaneously and remove, through the extrusion, cause the inside unnecessary defective material of separating of spacing collection frame to store in waste liquid collecting pipe department, thereby realized comparatively simple and handled unnecessary parting medium, and guarantee that the separation of mixed liquid rationally goes on.
3. This equipment and extraction process convenient to extract preparation adipose-derived stem cell exosome, the material through collecting is through piling up the extrusion gasbag frame, causes the gasbag frame to drive in cardboard frame and steady rest buckle separation turning block's the recess through the gas column, then increases its inside rotation steady force, and the sediment after so far separating is adipose-derived stem cell exosome to realized having improved the stability of key matter liquid separation, improved adipose-derived stem cell exosome's collection efficiency.
Drawings
FIG. 1 is a schematic view of the outer frame structure of the apparatus of the present invention;
FIG. 2 is a schematic diagram of an extraction head according to the present invention;
FIG. 3 is a schematic view of a separate adjustment frame according to the present invention;
FIG. 4 is a schematic view of the structure of the separation frame of the present invention;
FIG. 5 is a schematic view of the supporting compact of the present invention;
FIG. 6 is a schematic view of a pressurized stabilizer according to the present invention;
FIG. 7 is a schematic view of the gas column structure of the present invention.
In the figure: 1. an equipment outer frame; 101. a placement frame; 102. a push rod; 2. separating the adjusting frame; 201. a rotating wheel; 202. a pushing block; 203. a central rotating rod; 204. a sliding connecting rod; 205. a waste liquid collecting pipe; 3. a clutch release block; 301. an air bag holder; 302. a slide rail; 4. extracting a head; 401. a pressure runner; 402. a slide bar; 403. supporting the pressing block; 404. extruding the block; 405. a push rod; 406. a gas tube stand; 5. a separation frame; 501. a limiting collection frame; 502. a clamping plate; 503. separating the turning block; 6. a pressurizing stabilizing frame; 601. a gas column; 602. a stabilizer frame; 603. a card board frame; 604. receiving the gas stick.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-4, in a first embodiment: an apparatus for conveniently extracting and preparing adipose-derived stem cell exosomes comprises a separation adjusting frame 2, an extraction head 4 and a separation frame 5, wherein the separation adjusting frame 2 comprises a pushing block 202, two sides of the pushing block 202 are movably connected with a rotating wheel 201 for adjusting a descending position, one side of the pushing block 202, far away from the rotating wheel 201, is movably connected with a central rotating rod 203, two sides of the central rotating rod 203 are respectively provided with a sliding connecting rod 204 for adjusting pressure, one side of the pushing block 202 is provided with a waste liquid collecting pipe 205 with constant air pressure, the extraction head 4 comprises a pressure rotating wheel 401, the outer side of the pressure rotating wheel 401 is movably connected with a sliding rod 402 for telescoping, the sliding rod 402 is arranged at the bottom end port of the waste liquid collecting pipe 205, the top end of the pressure rotating wheel 401 is movably connected with a push rod 405, the inner side of the push rod 405 is provided with an air pipe frame 406 for collecting cells, the separation adjusting frame 2 is arranged at the bottom end of an apparatus outer frame 1, the separation frame 5 comprises a limit collection frame 501, a position clamping plate 502 is fixedly connected to the outer side of the limit collection frame 501, a separation rotating block 503 is arranged on one side, away from the position clamping plate 502, of the limit collection frame 501, the separation frame 5 is arranged on one side of the separation adjustment frame 2, the position clamping plate 502 is connected to one side of the waste liquid collection pipe 205 in a clamping mode, the pressure rotating wheel 401 is matched with the sliding rod 402 to increase the internal pressure, the collection stability of mixed liquid is improved, the collection speed of the mixed liquid is slowed down, the mixed liquid is quickly separated through the matching of the separation rotating block 503 and the limit collection frame 501, the stability of the device is ensured, the vesicle crushing in the process of collecting the exosomes by a low-temperature high-speed centrifugation method is avoided, more exosomes are obtained in a shorter time, the number of the pressure rotating wheels 401 is multiple groups, the multiple groups of pressure rotating wheels 401 are arranged on the surface of the sliding rod 402 in a linear array mode, and the top end port of the extrusion block 404 is movably connected with a rubber sleeve, the rubber sleeve of the extrusion block 404 and the push rod 405 are arranged in a sleeved mode, transmission of mixed liquid at each stage is guaranteed, the volume of the mixed liquid obtained at each time is fixed, the cross-sectional area of the outer side of the separation rotating block 503 is smaller than that of the side of the limiting collection frame 501, the side of the limiting collection frame 501 is matched with the outer side of the separation rotating block 503, the clamping plate frames 603 are arranged on two sides of the separation rotating block 503, and stability of the separation rotating block 503 during rotation is improved.
Please refer to fig. 5-7, example two: the outer side of the separation adjusting frame 2 is movably connected with an equipment outer frame 1, one side of the equipment outer frame 1, which is far away from the separation adjusting frame 2, is movably connected with a clutch separating block 3 for collecting cells, the structural linkage of the whole equipment is strengthened, and the equipment outer frame is easy to disassemble, the outer side of the separating frame 5 is movably connected with a pressurizing stabilizing frame 6 for storing the separated cells, the pressurizing stabilizing frame 6 comprises an air column 601, two sides of the air column 601 are movably connected with stabilizing frames 602 for expansion, the outer side of the stabilizing frame 602 is movably connected with a clamping plate frame 603, the top end of the air column 601 is movably connected with an air receiving rod 604, the air receiving rod 604 and the air column 601 are arranged in a sealing state, the air column 601 is arranged at the top end port of the limiting collecting frame 501, the adaptability of the clamping plate frame 603 to the separation rotating block 503 is improved through the matching of the air column 601 and the clamping plate frame 603, the smoothness of the separation rotating block 503 is improved, the equipment outer frame 1 comprises a placing frame 101, the top end of the placing frame 101 is movably connected with a push rod 102 used for controlling the sampling amount, and the push rod 102 is arranged at the top end of the separation rotating block 503.
Please refer to fig. 4-7, example three: clutch separation piece 3 includes air bag frame 301, there is the slide rail 302 that is used for control pressure in air bag frame 301's the outside, slide rail 302 sets up the outside at air column 601, slide rail 302 is the encapsulated situation setting with air column 601, the mixed liquid of every different stages is separated through multilayer air bag frame 301, the post processing of being convenient for, air bag frame 301's quantity is the multiunit, multiunit air bag frame 301 is linear array and sets up on receiving the surface of gas pole 604, the port department of the upper and lower both sides of air bag frame 301 is provided with the runner pipe, the runner pipe of air bag frame 301 is the circulation state under air bag frame 301 receives extruded atmospheric pressure to promote, it is comparatively simple to make mixed liquid preserve.
An extraction process convenient for extracting and preparing adipose-derived stem cell exosomes comprises the following steps:
s1: suction of the mixed solution
At first through pulling catch bar 102, the mixed liquid is adsorbed through drawing head 4 through the inside suction of equipment, and the imbibition liquid drives pressure runner 401 through inside suction to compress simultaneously, and pressure runner 401 is continuously compressed through slide bar 402, because multiunit pressure runner 401 is compressed, its inside pressure increases gradually, cause the rising speed of mixed liquid to be slower, and comparatively steady, air pressure drives push rod 405 through extrusion pressing block 404 and extrudes to the outside, and trachea frame 406 is inside to be extruded gradually to inside air disappearance, until push rod 405 removes the bottom to trachea frame 406, then mixed liquid single absorption is accomplished, and through the inside gas volume restriction of trachea frame 406, represent that the mixed liquid volume of absorption is unanimous.
S2: first Clutch step
And then the adsorbed mixed liquid is subjected to first clutch through a circulation pipe of a first layer of air bag frame at the bottom end and a second layer of air bag frame, the clutch force is 380 plus 420Xg, the separation rotating block 503 is driven to rotate, the redundant cell liquid is separated to the limiting collection frame 501 through the clutch force, the limiting collection frame 501 passes through a small hole inside, and the mixed liquid is transmitted to the next layer through the supporting pressing block 403.
S3: second Clutch step
The circulation pipes of the third layer of air bag frame 301 and the fourth layer of air bag frame 301 perform a second clutch on the adsorbed mixed liquid, the clutch force is 1800 plus 2200xg, the redundant cellular liquid is separated onto the limiting collection frame 501 by the clutch force in the same way, the limiting collection frame 501 conducts the redundant cellular liquid to the limiting collection frame 501 through the internal small hole, the mixed liquid is conducted to the next layer through the supporting pressing block 403, then the pushing block 202 is pushed down, the pushing block 202 drives the sliding connecting rod 204 to deflect inwards on the central rotating rod 203 through the rotating wheel 201, and drives the limiting collection frame 501 to move outwards, and the redundant residual materials separated inside the limiting collection frame 501 are stored in the waste liquid collection pipe 205 through extrusion.
S4: third Clutch step
And finally, the circulation pipes of the fifth-layer air bag frame 301 and the sixth-layer air bag frame 301 perform second clutch on the adsorbed mixed liquid, the clutch force is 100000-140000Xg, the centrifugal force in the fifth-layer air bag frame 301 is far greater than that in the first two times, the collected materials are stacked and extruded into the air bag frame 301, so that the air bag frame 301 drives the clamping plate frame 603 and the stabilizing frame 602 to be tightly buckled in the groove of the separation rotating block 503 through the air column 601, the rotating stabilizing force in the fifth-layer air bag frame 301 is increased, and the separated sediment is the adipose-derived stem cell exosome.
The working principle is as follows: by pulling the push rod 102, the mixed liquid is absorbed by the extraction head 4 by the suction inside the device, meanwhile, the suction liquid drives the pressure rotating wheels 401 to compress through the internal suction force, and the pressure rotating wheels 401 are continuously compressed through the sliding rods 402, because the multiple groups of pressure rotating wheels 401 are compressed, the pressure inside the mixing chamber is gradually increased, so that the rising speed of the mixed liquid is slow and stable, meanwhile, the air pressure drives the push rod 405 to extrude outwards through the extrusion block 404, and the inside of the air tube frame 406 is gradually pressed until the internal air disappears, until the push rod 405 moves to the bottom end of the air tube frame 406, the single draw of mixed liquor is complete and is limited by the volume of gas inside the gas tube frame 406, indicating that the volume of mixed liquor drawn is consistent, therefore, the extraction quantity is relatively average, the overall extraction efficiency is relatively high, and the damage of the cells inside in the extraction process due to extrusion is avoided.
The absorbed mixed liquid is subjected to first clutch through circulation pipes of a first layer of air bag frame 301 and a second layer of air bag frame 301 at the bottom, the clutch force is 380 plus 420Xg, the separation rotating block 503 is driven to rotate through driving, the redundant cellular fluid is separated to the limiting collection frame 501 through the clutch force, the limiting collection frame 501 is transmitted to the next layer through a supporting pressing block 403 through an inner small hole, the absorbed mixed liquid is subjected to second clutch through circulation pipes of the third layer of air bag frame 301 and the fourth layer of air bag frame 301, the clutch force is 1800 plus 2200Xg, the redundant cellular fluid is separated to the limiting collection frame 501 through the clutch force, the limiting collection frame 501 transmits the redundant cellular fluid to the limiting collection frame 501 through the inner small hole, the mixed liquid is transmitted to the next layer through the supporting pressing block 403, then the pushing block 201 is pushed down, the pushing block 202 drives the sliding connecting rod 204 to deflect on the central rotating rod 203 through a rotating wheel, drive spacing collection frame 501 simultaneously and remove to the outside, through the extrusion, cause the inside unnecessary defective material that separates of spacing collection frame 501 to store in waste liquid collecting pipe 205 department to realized comparatively simply handling unnecessary parting liquid, and guaranteed that the separation of mixed liquid goes on rationally.
The circulation pipes of the fifth layer air bag frame 301 and the sixth layer air bag frame 301 perform second clutch on the adsorbed mixed liquid, the clutch force is 100000-140000xg, the centrifugal force in the fifth layer air bag frame 301 is far greater than that in the first two times, the collected materials are stacked and extruded into the air bag frame 301, so that the air bag frame 301 drives the clamping plate frame 603 and the stabilizing frame 602 to be tightly buckled in the groove of the separation rotating block 503 through the air column 601, the rotating stabilizing force in the fifth layer air bag frame is increased, the separated sediment is the adipose-derived stem cell exosome, the stability of key mass-liquid separation is improved, and the collection efficiency of the adipose-derived stem cell exosome is improved.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (9)
1. An equipment convenient to extract preparation adipose-derived stem cell exosome, includes separation alignment jig (2), draws head (4), separation jig (5), its characterized in that: the separation adjusting frame (2) comprises a pushing block (202), wherein rotating wheels (201) used for adjusting descending positions are movably connected to two sides of the pushing block (202), one side, away from the rotating wheels (201), of the pushing block (202) is movably connected with a central rotating rod (203), sliding connecting rods (204) used for adjusting pressure are arranged on two sides of the central rotating rod (203), a waste liquid collecting pipe (205) with constant air pressure is arranged on one side of the pushing block (202), the extraction head (4) comprises a pressure rotating wheel (401), a sliding rod (402) used for stretching and retracting is movably connected to the outer side of the pressure rotating wheel (401), the sliding rod (402) is arranged at the bottom end of the waste liquid collecting pipe (205), a push rod (405) is movably connected to the top end of the pressure rotating wheel (401), and an air pipe frame (406) used for collecting cells is arranged on the inner side of the push rod (405), separation alignment jig (2) set up the top of drawing head (4), separation jig (5) are including spacing collection frame (501), the outside fixedly connected with screens board (502) of spacing collection frame (501), one side that screens board (502) were kept away from in spacing collection frame (501) is provided with separation turning block (503), separation jig (5) set up the one side in separation alignment jig (2), one side in waste liquid collecting pipe (205) of screens board (502) joint.
2. The apparatus for facilitating extraction and preparation of adipose-derived stem cell exosomes according to claim 1, wherein: the cell separation device is characterized in that an equipment outer frame (1) is movably connected to the outer side of the separation adjusting frame (2), a clutch separation block (3) used for collecting cells is movably connected to one side, away from the separation adjusting frame (2), of the equipment outer frame (1), and a pressurization stabilizing frame (6) used for dividing cells for storage is movably connected to the outer side of the separation frame (5).
3. The apparatus for facilitating extraction and preparation of adipose-derived stem cell exosomes according to claim 2, wherein: pressure boost steady rest (6) are including gas column (601), the both sides swing joint of gas column (601) has steady rest (602) that is used for the expansion, the outside swing joint of steady rest (602) has cardboard frame (603), the top swing joint of gas column (601) has receipt gas pole (604), it is the encapsulated situation setting with gas column (601) to receive gas pole (604), gas column (601) sets up the top port in spacing collection frame (501).
4. The apparatus for facilitating extraction and preparation of adipose-derived stem cell exosomes according to claim 3, wherein: the equipment outer frame (1) comprises a placement frame (101), a push rod (102) used for controlling the sampling amount is movably connected to the top end of the placement frame (101), and the push rod (102) is arranged at the top end of the separation rotating block (503).
5. The apparatus for facilitating extraction and preparation of adipose-derived stem cell exosomes according to claim 4, wherein: clutch separation piece (3) are including air bag frame (301), there is slide rail (302) that is used for control pressure in the outside of air bag frame (301), slide rail (302) set up the outside at air column (601), slide rail (302) are the sealed state setting with air column (601).
6. The apparatus for facilitating extraction and preparation of adipose-derived stem cell exosomes according to claim 5, wherein: the quantity of gasbag frame (301) is the multiunit, multiunit gasbag frame (301) are linear array and set up on receiving the surface of gas pole (604), the port department of the upper and lower both sides of gasbag frame (301) is provided with the runner pipe, the runner pipe of gasbag frame (301) is the circulation state under gasbag frame (301) is promoted by extruded atmospheric pressure.
7. The apparatus for facilitating extraction and preparation of adipose-derived stem cell exosomes according to claim 6, wherein: the quantity of pressure runner (401) is the multiunit, multiunit pressure runner (401) are linear array and set up on the surface of slide bar (402), the top port department swing joint of extrusion piece (404) has the rubber sleeve, the rubber sleeve of extrusion piece (404) is the state setting of cup jointing with push rod (405).
8. The apparatus for facilitating extraction and preparation of adipose-derived stem cell exosomes according to claim 7, wherein: the lateral cross sectional area of separation turning block (503) is less than the lateral cross sectional area of spacing collection frame (501), the side of spacing collection frame (501) and the outside looks adaptation of separation turning block (503), cardboard frame (603) set up the both sides at separation turning block (503).
9. The extraction process for conveniently extracting and preparing the adipose-derived stem cell exosomes according to claim 8, comprising the following steps of:
s1: suction of the mixed solution
Firstly, by pulling a push rod (102), mixed liquid is adsorbed by an extraction head (4) through suction inside the equipment, meanwhile, the suction liquid drives a pressure rotating wheel (401) to be compressed through internal suction, the pressure rotating wheel (401) is continuously compressed through a sliding rod (402), as a plurality of groups of pressure rotating wheels (401) are compressed, the internal pressure of the pressure rotating wheels is gradually increased, so that the rising speed of the mixed liquid is slow and stable, meanwhile, the air pressure drives a push rod (405) to be extruded outwards through an extrusion block (404), the inside of a gas tube frame (406) is gradually extruded until the internal air disappears, until the push rod (405) moves to the bottom end of the gas tube frame (406), the single suction of the mixed liquid is completed, and the volume of the sucked mixed liquid is consistent through the limitation of the internal gas volume of the gas tube frame (406);
s2: first Clutch step
Then, the adsorbed mixed liquid is subjected to first clutch through circulation pipes of a first layer of air bag frame (301) and a second layer of air bag frame (301) at the bottom ends, the clutch force is 380-;
s3: second Clutch step
The circulation pipes of the third layer of air bag frame (301) and the fourth layer of air bag frame (301) perform second clutch on the adsorbed mixed liquid, the clutch force is 1800 plus 2200xg, redundant cellular liquid is separated to the limiting collection frame (501) by the clutch force, the limiting collection frame (501) conducts the redundant cellular liquid to the limiting collection frame (501) through an internal small hole, meanwhile, the mixed liquid is conducted to the next layer through the supporting pressing block (403), then the pushing block (202) is pushed down, the pushing block (202) drives the sliding connecting rod (204) to deflect inwards on the central rotating rod (203) through the rotating wheel (201), meanwhile, the limiting collection frame (501) is driven to move outwards, and redundant residual materials separated in the limiting collection frame (501) are stored in the waste liquid collection pipe (205) through extrusion;
s4: third Clutch step
And finally, the circulation pipes of the fifth layer of air bag frame (301) and the sixth layer of air bag frame (301) perform second clutch on the adsorbed mixed liquid, the clutch force is 100000-140000Xg, the centrifugal force in the fifth layer of air bag frame (301) is far greater than that in the first two times, the collected materials are stacked and extruded on the air bag frame (301), so that the air bag frame (301) drives the clamping plate frame (603) and the stabilizing frame (602) to be tightly buckled in the groove of the separation rotating block (503) through the air column (601), the rotating smooth force in the fifth layer of air bag frame is increased, and the separated sediment is the adipose-derived stem cell exosome.
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Application publication date: 20211224 |