CN219279846U - Exosome separation and extraction device - Google Patents

Abstract

The utility model belongs to the technical field of exosome processing equipment, and particularly relates to an exosome separation and extraction device which comprises a tank body, a placing plate, an exosome centrifugal test tube, a telescopic unit, a compressor and a driving unit, wherein the telescopic unit and the compressor are respectively arranged on the inner bottom surface of the tank body, the placing plate is rotationally connected with the inner wall of the tank body and is positioned above the telescopic unit, the exosome centrifugal test tube is arranged in the axial direction in the tank body, the bottom end of the exosome centrifugal test tube passes through the placing plate to be connected with the telescopic unit, the upper end of the exosome centrifugal test tube is positioned above the placing plate, the telescopic unit is coaxially arranged with the exosome centrifugal test tube, the telescopic unit is movably connected with the inner wall of the tank body and drives the exosome centrifugal test tube to slide up and down along the axial direction of the tank body, and the driving unit is arranged outside the tank body and is connected with the lower surface of the placing plate. The exosome separation and extraction device provided by the utility model has the advantages that the separation test tube is convenient to assemble and take, the operation temperature control can be realized, and the structure is simple.

Description

Exosome separation and extraction device

Technical Field

The utility model belongs to the technical field of exosome processing equipment, and particularly relates to an exosome separation and extraction device.

Background

The exosomes are nanospheres with a diameter of 40-100 nm and a double-layer membrane structure, and mainly comprise lipid, protein and genetic materials, the exosomes from different sources have specific surface molecules, play an important role in information transmission among cells and participate in the occurrence and development processes of various physiology and pathology, and the exosomes have the characteristic of easily penetrating cell membranes, so that the exosomes have natural advantages as carriers for drug delivery; the exosomes are used as drug delivery carriers, and the advantages of cell and nanotechnology drug delivery are combined, firstly, the exosomes are used as drug carriers, the safety is high, the exosomes are used as bioactive capsules, and the drug delivery cannot be deposited at different parts in the body to cause immune abnormal reaction; secondly, the exosome can improve the stability of the medicine, and protect the medicine from being rapidly decomposed in the in-vivo transportation process, so that the in-vivo acting time of the medicine is prolonged; thirdly, the exosomes are nanoscale molecules and carry cell surface substances, so that the exosomes have strong capacity of penetrating various biological barriers; fourth, exosomes have a natural targeting ability based on donor cells.

The exosome can be used as carrier to load functional substances, such as glabridin, which is a plant estrogen, the biological activity of which comprises the functions of antioxidation, anti-inflammation, neuroprotection, anti-atherosclerosis, energy metabolism regulation and the like, and also comprises anti-tumor, anti-nephritis and the like. The exosome loaded glabridin belongs to a carrier technology, and the technology is the most advanced whitening or treatment technology for researching glabridin at present.

The existing exosome extraction methods are more, and the common methods include kit extraction and ultracentrifugation; for the ultracentrifugation method, the size, density and other differences of the exosomes and surrounding substances are mainly utilized to separate the exosomes, and then the exosomes are further utilized to wrap the functional substances, so that the bioavailability of the functional substances is improved. Although the existing ultracentrifugation method can realize separation and extraction of exosomes, most of devices adopted in the centrifugation are centrifuges, before ultracentrifugation, the devices need to extend into the centrifuges, test tubes with exosomes are filled into the centrifuges, and the loading and unloading operations are inconvenient because the depth of the placement position of the centrifuge tube is deeper, so that the centrifuge tube is inconvenient to observe; because different environment temperatures are needed for the centrifugal separation of the exosomes or the exosomes are needed to be heated, the heating structure of the existing device is complex; the existing separation time is long, and the separation purity is low.

Disclosure of Invention

Aiming at the technical problems of inconvenient assembly and disassembly operations and complex structure of the existing exosome separation and disassembly, the utility model provides the exosome separation and disassembly device, which is convenient for assembly and disassembly operations of a separation test tube, can realize operation temperature control and has a simple structure.

In order to achieve the above purpose, the technical scheme adopted by the utility model is as follows:

the utility model provides an exosome separation extraction element, includes jar body, places board, exosome centrifugation test tube, flexible unit, compressor and drive unit, flexible unit and compressor are arranged in respectively on the interior bottom surface of jar body, place the board and rotate with the inner wall of jar body and be connected and be located flexible unit top, exosome centrifugation test tube is arranged in the internal axial direction of jar on, the bottom of exosome centrifugation test tube is passed and is placed the board and be connected with flexible unit, the upper end of exosome centrifugation test tube is located and places the board top, flexible unit and exosome centrifugation test tube coaxial arrangement, flexible unit still with the inner wall swing joint of jar body and drive exosome centrifugation test tube along jar axial oscilaltion of body, drive unit is arranged in the jar body and is connected with the lower surface of placing the board.

Further, the telescopic unit comprises a fixed block and a cylinder which are connected up and down; the cylinder is arranged on the inner bottom surface of the tank body and is coaxially arranged with the exosome centrifugal test tube; the fixed block is located and places the board below and with the inner wall swing joint of jar body, set up the U-shaped recess on the fixed block upper surface, set up rubber gasket on the U-shaped recess internal surface, place the board on and set up the jack, the bottom of the centrifugal test tube of exosome passes the jack card and goes into in the U-shaped recess and contact with rubber gasket.

Further, the telescopic unit further comprises a sliding groove, a sliding block and a fixing rod, wherein the sliding groove is arranged on the inner wall of the tank body and is positioned below the placing plate, the sliding block is arranged in the sliding groove, the axial direction of the fixing rod is perpendicular to the axial direction of the air cylinder, the axial direction of the sliding groove is parallel to the axial direction of the air cylinder, and the fixing block is connected with the sliding block through the fixing rod.

Further, a sealing cover is arranged on the top of the tank body; a sealing plug is arranged on the pipe orifice of the exosome centrifugal test tube, and a clamping block is arranged on the outer wall of the exosome centrifugal test tube; the fixture blocks are one or more, and the plurality of fixture blocks are uniformly distributed along the circumference of the exosome centrifugal test tube.

Further, the centrifugal test tube of exosome and flexible unit are a plurality of that the quantity equals, and a plurality of exosome centrifugal test tubes are connected with a plurality of flexible unit one-to-one, and a plurality of exosome centrifugal test tubes are circumference distribution along the center pin of jar body.

Further, the driving unit comprises a power box, a rotating shaft and a motor, wherein the rotating shaft and the motor are respectively arranged in the power box; the power box is arranged on the outer bottom surface of the tank body, the fixing frame is arranged on the lower surface of the placing plate, the fixing frame and the rotating shaft are coaxially arranged with the tank body, one end of the rotating shaft is connected with the motor, and the other end of the rotating shaft is connected with the fixing frame.

Further, the exosome separation and extraction device further comprises a first sliding groove which is arranged on the inner wall of the tank body and is positioned above the sliding groove, a first sliding block is arranged on the outer wall of the placing plate, and the first sliding block is clamped into the first sliding groove and rotates in the first sliding groove.

Further, the exosome separation and extraction device further comprises rotating units arranged in the placing plate, wherein the rotating units are arranged in pairs and are distributed in a mirror image mode relative to the axial direction of the jack, and the rotating units are in contact with the outer wall of the exosome centrifugal test tube;

the rotating unit comprises a second sliding block, a second sliding groove, a spring and a rotating wheel; the second sliding groove is arranged in the placing plate, the second sliding block and the spring are both arranged in the second sliding groove, and the rotating wheel is positioned outside the second sliding groove; one end of the second sliding block is connected with the inner wall of the second sliding groove through a spring, the other end of the second sliding block extends out of the second sliding groove to be connected with a rotating wheel, and the rotating wheel is contacted with the outer wall of the exosome centrifugal test tube.

Further, the rotating units are in a plurality of pairs, and the plurality of pairs of rotating units are uniformly distributed along the axial direction of the jack.

Further, the exosome separation and extraction device further comprises an ultrasonic probe arranged at the bottom of the exosome centrifugal test tube and an ultrasonic power supply arranged outside the tank body, and the ultrasonic power supply is connected with the ultrasonic probe.

The beneficial effects of the utility model are as follows:

1. in the utility model, the placing plate is positioned above the tank body, and the exosome centrifugal test tube on the placing plate stretches up and down in the axial direction of the tank body through the stretching unit, so that the loading and taking process of the exosome centrifugal test can be conveniently observed, and the operation is convenient; simultaneously set up the compressor in the jar internal, come the inside temperature of control jar through the compressor, and heating device is simple, and can satisfy the separation of the exosome and draw the operation to the demand of temperature.

2. In the utility model, the driving unit comprises a rotating shaft, a power box and a motor; the power box is arranged on the outer bottom surface of the tank body, the motor is arranged in the power box, the fixing frame is arranged on the lower surface of the placing plate, the fixing frame and the rotating shaft are coaxially arranged with the tank body, one end of the rotating shaft is connected with the motor, and the other end of the rotating shaft is connected with the fixing frame. When the rotating shaft is driven by the motor to rotate, the fixing frame and the placing plate are driven to synchronously rotate along with the rotating shaft, the exosome centrifugal test tube placed on the placing plate also synchronously rotates, and meanwhile, the placing plate drives the first sliding block to rotate in the first sliding groove when rotating, so that the exosome inside the exosome centrifugal test tube is separated at a high speed.

3. In the utility model, a rotating unit is arranged in a placing plate, the rotating units are arranged in pairs relative to the axial direction of a jack, and the rotating units are contacted with the outer wall of an exosome centrifugal test tube; in the process of loading and taking the exosome centrifugal test tube, the stability of the exosome centrifugal test tube in the up-and-down movement is improved through the rotation of the rotation unit.

4. According to the utility model, the ultrasonic probe is arranged at the inner bottom of the exosome centrifugal test tube, the ultrasonic power supply is arranged at the outer part of the tank body, and the ultrasonic power supply is connected with the ultrasonic probe and sends excitation voltage to the ultrasonic probe, so that the ultrasonic probe can release low-frequency ultrasonic waves, the exosome in the exosome centrifugal test tube is subjected to ultrasonic treatment, the separation and extraction speed of the exosome is accelerated under the action of ultrasonic waves and high-speed rotation, the separation and extraction efficiency and the separation purity are improved, and the technical problems of long separation time and low separation purity in the prior art are solved.

Drawings

FIG. 1 is a schematic view of a front cross-sectional structure of the present utility model;

FIG. 2 is a schematic view of the external structure of the present utility model;

FIG. 3 is a schematic top cross-sectional view of the tank of the present utility model;

FIG. 4 is an enlarged schematic view of the structure of FIG. 1A according to the present utility model;

FIG. 5 is a schematic view of the internal structure of the power box of the present utility model;

in the figure:

1-a tank body; 2-placing a plate; 3-exosome centrifugation tube; 4-a fixing frame; 5-a chute; 6-a sliding block; 7-a rubber gasket; 8, an air cylinder; 9-a rotating shaft; 10-a power box; 11-a compressor; 12-a fixed rod; 13-a fixed block; 14-a first slider; 15-jack; 16-a first sliding groove; 17-a second slider; 18-a second sliding groove; 19-a spring; 20-rotating wheels; 21-a motor; 22-sealing cover.

Detailed Description

The technical solution of the present utility model will now be described clearly and fully with reference to the accompanying drawings and specific examples, but the examples are only preferred embodiments of the technical solution of the present utility model, and not all embodiments. Based on the embodiments provided by the utility model, other embodiments obtained by a person of ordinary skill in the art without making creative efforts belong to the protection scope of the technical scheme of the utility model.

Examples

An exosome separation and extraction device comprises a tank body 1, a placing plate 2, an exosome centrifugal test tube 3, a telescopic unit, a compressor 11 and a driving unit.

Specifically, referring to fig. 1, 2 and 3, the tank body 1 is a cylindrical tank, a sealing cover 22 is arranged on a tank opening at the top of the tank body 1, the tank body 1 is completely sealed by the sealing cover 22, the sealing cover 22 is movably connected with the tank body 1, a handle is arranged on the sealing cover 22, and the sealing cover 22 is convenient to open and close.

The compressor 11 is provided on the inner bottom surface of the tank 1, the compressor 11 is a conventional commercial product, and the temperature inside the tank 1 is controlled by the compressor 11.

Referring to fig. 1, in the inside of the tank body 1, a placing plate 2 is arranged near the tank opening, the placing plate 2 is rotationally connected with the inner wall of the tank body 1, the placing plate 2 is a circular plate, the plate surface of the placing plate 2 is vertical to the axial direction of the tank body 1, and the placing plate 2 and the tank body 1 are coaxially arranged.

Referring to fig. 3, the insertion holes 15 are formed in the axial direction of the placement plate 2, the insertion holes 15 are multiple and are uniformly distributed on the plate surface of the placement plate 2, and the connecting line of the circle centers of the insertion holes 15 is circular. The insertion hole 15 is located in the outer circumferential direction of the mount 4.

Referring to fig. 1 and 3, a first sliding groove 16 is provided on the inner wall of the can 1, and the placement plate 2 and the first sliding block 14 are on the same level; the outer wall of the placing plate 2 is provided with the first sliding block 14, the first sliding block 14 is clamped into the first sliding groove 16 and rotates in the first sliding groove 16, when the rotating shaft 9 drives the placing plate 2 to rotate, the first sliding block 14 is driven to rotate in the first sliding groove 16, high-speed rotation of the exosome centrifugal test tube 3 is achieved, and the exosome inside the exosome centrifugal test tube 3 is separated at high speed.

Referring to fig. 1, a fixing frame 4 is mounted on the lower surface of the placement plate 2, the fixing frame 4 is of a U-shaped structure, and the fixing frame 4, the placement plate 2 and the tank body 1 are coaxially arranged. The placing plate 2 is connected with the rotating shaft 9 through the fixing frame 4.

The axial of the exosome centrifugation test tube 3 and the jar body 1 is the same, and the bottom of exosome centrifugation test tube 3 passes to place board 2 and flexible unit connection, and place board 2 top is placed on the top of exosome centrifugation test tube 3. Specifically, the centrifugal test tube 3 of exosome passes jack 15 and is connected with flexible unit, sets up the sealing plug on the mouth of pipe of the centrifugal test tube 3 of exosome, and flexible unit and the coaxial setting of the centrifugal test tube 3 of exosome, flexible unit drive the centrifugal test tube 3 of exosome slide from top to bottom along the axial of jar body 1.

The exosome centrifugation test tube 3 is cylindrical, sets up the fixture block on the outer wall of exosome centrifugation test tube 3, can make exosome centrifugation test tube 3 card place board 2 up end, be difficult for dropping. The height of the clamping block from the pipe orifice is 1/3-3/5 of the height of the exosome centrifugal test tube.

In order to improve the steadiness of the centrifugal test tube of exosome 3, the fixture block is 2 at least, and the equipartition is on the centrifugal test tube of exosome 3 circumferencial direction, and the centrifugal test tube of exosome 3 can be stable the card place on board 2, can also break away from fixed block 13 in order to make the bottom of the centrifugal test tube of exosome 3 easily simultaneously.

Referring to fig. 1, the telescopic unit includes a fixed block 13 and a cylinder 8 connected up and down; the air cylinder 8 is arranged on the inner bottom surface of the tank body 1, and the air cylinder 8 and the exosome centrifugal test tube 3 are coaxially arranged; the fixed block 13 is located and places board 2 below and with jar body 1 swing joint, has seted up the U-shaped recess on the fixed block 13 upper surface, sets up rubber gasket 7 on the U-shaped recess internal surface, and the bottom of exosome centrifugation test tube 3 passes jack 15 card and goes into the U-shaped recess in and contact with rubber gasket 7.

Referring to fig. 1, the telescopic unit further comprises a sliding groove 5, a sliding block 6 and a fixing rod 12, wherein the sliding groove 5 is arranged on the inner wall of the tank body 1 and is positioned below the placing plate 2, the sliding block 6 is arranged in the sliding groove 5, the fixing rod 12 is axially perpendicular to the air cylinder 8, the axial direction of the sliding groove 5 is axially parallel to the air cylinder 8, one end of the fixing rod 12 is connected with a fixing block 13, the other end of the fixing rod 12 is connected with the sliding block 6, and the fixing block 13 is connected with the sliding block 6 through the fixing rod 12.

The cylinder 8 is externally connected with a power supply, when the cylinder 8 is started, the fixing block 13 is driven to move upwards through the upward movement of the telescopic end of the cylinder 8, the fixing rod 12 connected with the fixing block 13 also moves upwards, the sliding block 6 is driven to slide upwards in the sliding groove 5 until the top end of the fixing block 13 is mutually abutted with the lower end face of the placing plate 2, then the exosome centrifugal test tube 3 with the exosome is placed into the inserting hole 15, the bottom end of the exosome centrifugal test tube 3 is contacted with the top end of the rubber gasket 7, the sealing cover 22 is covered above the tank 1, then the fixing block 13 is driven to move downwards sequentially through the reverse control cylinder 8, the exosome centrifugal test tube 3 is driven to move downwards until the exosome centrifugal test tube 3 is blocked in the inserting hole 15 and does not move downwards, the cylinder 8 continues to rotate downwards in a reverse direction until the bottom end of the exosome centrifugal test tube 3 is separated from the rubber gasket 7, and is separated from the U-shaped groove on the fixing block 13, and the loading of the exosome centrifugal test tube 3 is completed. The exosome centrifuge tube 3 is pushed out according to the reverse operation described above, and then the exosome centrifuge tube 3 is taken out from the placement plate 2.

The centrifugal test tube of exosome 3 and flexible unit are a plurality of that the quantity equals, and a plurality of exosome centrifugal test tubes 3 are connected with a plurality of flexible units one-to-one, and every exosome centrifugal test tube 3 is placed in a jack 15, and a plurality of exosome centrifugal test tubes 3 are circumference distribution along the center pin of jar body 1, and every exosome centrifugal test tube 3 lower extreme corresponds and connects a flexible unit, and a plurality of flexible units synchronous flexible removal.

The lower bottom surface of the tank body 1 is provided with a driving unit, and the driving unit is connected with the lower surface of the placing plate 2 arranged in the tank body 1 to drive the placing plate 2 to rotate at a high speed.

Specifically, referring to fig. 1, 2 and 5, the driving unit includes a power box 10, and a rotation shaft 9 and a motor 21 respectively disposed in the power box 10; the power box 10 is arranged on the outer bottom surface of the tank body 1, the fixing frame 4 is arranged on the lower surface of the placing plate 2, the fixing frame 4 and the rotating shaft 9 are coaxially arranged with the tank body 1, one end of the rotating shaft 9 is connected with the motor 21, the other end of the rotating shaft 9 is connected with the fixing frame 4, and the placing plate 2 is driven to rotate at a high speed.

In the implementation, the power box 10 and the tank body 1 form a welding structure integration, so that the stability is improved.

Further, referring to fig. 1 and 4, the exosome separation and extraction device further includes a rotation unit disposed in the placement plate 2, the rotation units are disposed in pairs and are distributed in mirror images with respect to the axis of the insertion hole 15, and the rotation units are all in contact with the outer wall of the exosome centrifugal test tube 3.

Referring to fig. 4, the rotating unit includes a second slider 17, a second slider groove 18, a spring 19, and a rotating wheel 20; the second sliding groove 18 is arranged in the placing plate 2, the second sliding block 17 and the spring 19 are both arranged in the second sliding groove 18, and the rotating wheel 20 is positioned outside the second sliding groove 18; one end of the second sliding block 17 is connected with the inner wall of the second sliding groove 18 through a spring 19, the other end of the second sliding block 17 extends out of the second sliding groove 18 to be connected with a rotating wheel 20, and the rotating wheel 20 is contacted with the outer wall of the exosome centrifugal test tube 3.

The second sliding groove 18, the spring 19 and the second sliding block 17 are coaxially arranged in the radial direction of the tank 1, that is, the axial direction of the spring 19 is perpendicular to the axial direction of the exosome centrifugal test tube 3, so that when the rotating wheel 20 rotates, the spring 19 and the second sliding block 17 generate thrust in the horizontal direction.

The rotation units are multiple pairs, and the multiple pairs of rotation units are uniformly distributed up and down along the axial direction of the jack 15. In this embodiment, the rotation units are two pairs, the two pairs of rotation units are distributed up and down along the axial direction of the jack 15, and the rotation units in each pair are distributed in a mirror image manner with respect to the axial direction of the jack 15, so that the outer parts of the exosome centrifugal test tube 3 are ensured to be contacted with the rotation wheel 20.

When the cylinder 8 drives the exosome centrifugal test tube 3 to move up and down along the axial direction of the tank 1, the rotating wheel 20 and the second sliding block 17 are sequentially driven to rotate, and the rotating force generated when the second sliding block 17 rotates acts on the spring 19, so that the spring 19 in the second sliding groove 18 generates horizontal pushing force to the second sliding block 17, and the horizontal pushing force is transmitted to the rotating wheel 20 through the second sliding block 17, thereby ensuring that the exosome centrifugal test tube 3 can stably move and enhancing the stability of the device.

Furthermore, in order to solve the problems of low separation efficiency and low purity of the existing exosome, the exosome separation and extraction device of the utility model further comprises an ultrasonic probe arranged at the bottom in the exosome centrifugal test tube 3 and an ultrasonic power supply arranged outside the tank body 1, wherein the ultrasonic power supply is connected with the ultrasonic probe.

The ultrasonic probe is a current commercial product, and the ultrasonic probe is used for releasing low-frequency ultrasonic waves in a medium. The exosome raw materials to be separated are placed in an exosome centrifugal test tube 3, then an ultrasonic probe is placed in the exosome centrifugal test tube, an ultrasonic power supply sends excitation voltage to the ultrasonic probe, the ultrasonic probe releases low-frequency ultrasonic waves, the exosome raw materials can be subjected to ultrasonic treatment, the separation and extraction speed of the exosome is accelerated under the ultrasonic action and the high-speed rotation action, and the separation and extraction efficiency and the separation purity are improved.

Under the condition that the position relation, the connection relation and the movement state of the components are clear, the dimensional relation among the devices can be designed according to the mechanical requirement during high-speed rotation, so that the exosome centrifugal test tube 3 is not easy to break under the high-speed rotation, the exosome centrifugal test tube 3 can extend out of the tank body 1 under the maximum extension length of the air cylinder 8, the observation is convenient, and the loading and the unloading of the exosome centrifugal test tube 3 are also convenient. In addition, the materials of the components in the utility model are selected, especially the placing plate 2, the exosome centrifugal test tube 3, the fixing frame 4 and the like, so that the materials meet the strength requirement during high-speed rotation, and the safety and stability during high-speed rotation are ensured.

When the separation and extraction device provided by the utility model is used for separating exosomes at high speed, firstly, the sealing cover 22 at the top end of the tank body 1 is opened, the cylinder 8 is started to work forwards by using an external power supply, the cylinder 8 continuously stretches out and drives the fixed block 13 to move upwards, meanwhile, the fixed rod 12 also moves upwards and drives the sliding block 6 to slide upwards in the sliding groove 5 until the top end of the fixed block 13 is mutually abutted with the bottom end of the placing plate 2, then the exosome centrifugal test tube 3 (filled with exosome raw materials to be separated) is placed in the jack 15, the bottom end of the exosome centrifugal test tube 3 is contacted with the rubber gasket 7 and clamped into the U-shaped groove, at the moment, the top end of the exosome centrifugal test tube 3 stretches out of the tank body 1 and continues to control the cylinder 8 to work backwards, the cylinder 8 stretches downwards, and the exosome centrifugal test tube 3 continues to move downwards along the axial direction of the cylinder 8 under the action of the fixed block 13 until the clamping block on the outer wall of the exosome centrifugal test tube 3 is clamped on the upper surface of the placing plate 2, and the sealing cover 22 covers the tank opening of the tank body 1; then the cylinder 8 continues to retract downwards, and as the exosome centrifugation test tube 3 is clamped on the placing plate 2, only the fixed block 13 continues to move downwards along with the cylinder 8 until the exosome centrifugation test tube 3 is separated from the fixed block 13, and the cylinder 8 retracts lowest; then external power supply starts motor 21 to drive axis of rotation 9 and rotates, axis of rotation 9 drives mount 4 in proper order and places board 2 rotation, place the inside of board 2 on the first sliding block 14 along jar body 1 radial direction rotation in first sliding tray 16, place board 2 on the centrifugal test tube of exosome 3 along with placing board 2 high-speed rotation for the exosome in the centrifugal test tube of exosome 3 separates at a high speed, motor 21 rotates, ultrasonic power supply sends excitation voltage to ultrasonic transducer, ultrasonic transducer releases low-frequency ultrasonic wave, under ultrasonic wave and high-speed pivoted dual effect, accelerate the separation extraction rate of exosome, the exosome purity of separation is high, separation extraction efficiency is high. And then, carrying the separated and purified exosomes and glabridin, and wrapping the glabridin in the exosomes, so as to accelerate the absorption speed of cells and the bioavailability of the glabridin.

Finally, the exosome centrifuge tube 3 is removed after the high-speed separation. Firstly, the power supply of the motor 21 is turned off, the ultrasonic power supply is turned off, the sealing cover 22 is turned on, the cylinder 8 is started to rotate positively, the fixed block 13 is driven by the cylinder 8 to move upwards to contact with the bottom of the exosome centrifugal test tube 3, the cylinder 8 continues to move upwards, the exosome centrifugal test tube 3 is ejected upwards and extends out of the tank opening of the tank body 1, and the exosome centrifugal test tube 3 is conveniently taken out.

Meanwhile, in the present utility model, when the temperature needs to be regulated in the process of high-speed separation of the exosomes, the temperature in the tank 1 is controlled by the compressor 11. In addition, when the exosome centrifugation test tube 3 moves upwards or downwards, the movement of the exosome centrifugation test tube 3 drives the rotating wheel 20 to rotate, the rotating force is transmitted to the spring 19 in the second sliding groove 18 through the second sliding block 17, a reverse pushing force is generated on the second sliding block 17 under the action of the spring 19, and the pushing force is transmitted to the rotating wheel 20 through the second sliding block 17, so that the stability of the exosome centrifugation test tube 3 during movement is improved through the rotation of the rotating wheel 20.

The separation and extraction device provided by the utility model can also be used for storing the separation and purification exosomes, and the temperature in the tank body 1 is controlled by the compressor 11 during storage, so that the exosomes are stored.

In this embodiment, the centrifugal test tube 3 of exosome is blocked on placing the board 2 through the fixture block, further guarantee the stability of the centrifugal test tube 3 of exosome when high-speed rotation, avoid exosome centrifugal test tube 3 to split, can also be on the basis of above-mentioned embodiment, below and spout 5's the top department of placing the board 2, the diaphragm that increases again, the diaphragm is parallel to and sets up with placing the board 2, the diaphragm rotates with the inner wall of jar body 1 to be connected (can refer to the rotation connection structure of placing the board 2), the diaphragm is the ring board, set up the through-hole on the diaphragm, the position of through-hole corresponds with the position of jack 15, the mount 4 upwards passes the inner ring on the diaphragm and is connected with the lower surface of placing the board 2, and the lateral wall of mount 4 is connected with the diaphragm, the bottom of exosome centrifugal test tube 3 passes the jack 15 on placing the board 2 earlier, pass the through-hole on the diaphragm and the contact of the U-shaped recess of fixed block 13 again.

Claims (10)

1. The utility model provides an exosome separation extraction element, its characterized in that, include jar body (1), place board (2), exosome centrifugation test tube (3), flexible unit, compressor (11) and drive unit, flexible unit and compressor (11) are arranged in respectively on the interior bottom surface of jar body (1), place the inboard rotation of board (2) and jar body (1) and be connected and be located flexible unit top, exosome centrifugation test tube (3) are arranged in the axial direction in jar body (1), place board (2) and flexible unit connection are passed in the bottom of exosome centrifugation test tube (3), the upper end of exosome centrifugation test tube (3) is located places board (2) top, flexible unit and exosome centrifugation test tube (3) coaxial setting, flexible unit still with the inner wall swing joint of jar body (1) and drive exosome centrifugation test tube (3) slide from top to bottom along the axial of jar body (1), drive unit arranges jar body (1) and is connected with the lower surface of placing board (2).

2. The exosome separation and extraction device according to claim 1, wherein the telescopic unit comprises a fixed block (13) and a cylinder (8) which are connected up and down; the cylinder (8) is arranged on the inner bottom surface of the tank body (1), and the cylinder (8) and the exosome centrifugal test tube (3) are coaxially arranged; the utility model discloses a jar body, including fixed block (13), rubber gasket (7) and rubber gasket (7), fixed block (13) are located to place board (2) below and with the inner wall swing joint of jar body (1), set up U-shaped recess on fixed block (13) upper surface, set up jack (15) on placing board (2), jack (15) card is passed in U-shaped recess and with rubber gasket (7) contact are passed to the bottom of exosome centrifugation test tube (3).

3. The exosome separation and extraction device according to claim 2, wherein the telescopic unit further comprises a sliding groove (5), a sliding block (6) and a fixing rod (12), the sliding groove (5) is arranged on the inner wall of the tank body (1) and is positioned below the placing plate (2), the sliding block (6) is arranged in the sliding groove (5), the fixing rod (12) is axially perpendicular to the air cylinder (8), the axial direction of the sliding groove (5) is axially parallel to the air cylinder (8), and the fixing block (13) is connected with the sliding block (6) through the fixing rod (12).

4. -exosome separation and extraction device according to claim 3, characterized in that a sealing cover (22) is provided on top of the tank (1); a sealing plug is arranged on a pipe orifice of the exosome centrifugal test tube (3), and a clamping block is arranged on the outer wall of the exosome centrifugal test tube (3); the clamping blocks are one or more, and the clamping blocks are uniformly distributed along the circumference of the exosome centrifugal test tube (3).

5. The exosome separation and extraction device according to claim 4, wherein the number of the exosome centrifugal test tubes (3) and the number of the telescopic units are equal, the plurality of exosome centrifugal test tubes (3) are connected with the plurality of telescopic units in a one-to-one correspondence manner, and the plurality of exosome centrifugal test tubes (3) are circumferentially distributed along the central axis of the tank body (1).

6. The exosome separation and extraction device according to claim 5, wherein the drive unit comprises a power box (10), and a rotating shaft (9) and a motor (21) respectively arranged in the power box (10); the power box (10) is arranged on the outer bottom surface of the tank body (1), the fixing frame (4) is arranged on the lower surface of the placing plate (2), the fixing frame (4) and the rotating shaft (9) are coaxially arranged with the tank body (1), one end of the rotating shaft (9) is connected with the motor (21), and the other end of the rotating shaft (9) is connected with the fixing frame (4).

7. The exosome separation and extraction device according to claim 6, further comprising a first sliding groove (16) arranged on the inner wall of the tank body (1) and located above the sliding groove (5), wherein a first sliding block (14) is arranged on the outer wall of the placing plate (2), and the first sliding block (14) is clamped into the first sliding groove (16) and rotates in the first sliding groove (16).

8. Exosome separation and extraction device according to any one of claims 2-7, further comprising rotation units arranged in the placement plate (2), the rotation units being arranged in pairs and being mirror-image distributed with respect to the axial direction of the insertion holes (15), the rotation units being in contact with the outer wall of the exosome centrifugation tube (3);

the rotating unit comprises a second sliding block (17), a second sliding groove (18), a spring (19) and a rotating wheel (20); the second sliding groove (18) is arranged in the placing plate (2), the second sliding block (17) and the spring (19) are both arranged in the second sliding groove (18), and the rotating wheel (20) is positioned outside the second sliding groove (18); one end of the second sliding block (17) is connected with the inner wall of the second sliding groove (18) through a spring (19), the other end of the second sliding block (17) extends out of the second sliding groove (18) to be connected with a rotating wheel (20), and the rotating wheel (20) is contacted with the outer wall of the exosome centrifugal test tube (3).

9. The exosome separation and extraction device according to claim 8, wherein the rotation units are multiple pairs, and the multiple pairs of rotation units are uniformly distributed along the axial direction of the insertion hole (15).

10. The exosome separation and extraction device according to claim 9, further comprising an ultrasonic probe disposed at the inner bottom of the exosome centrifugation tube (3) and an ultrasonic power supply disposed outside the tank (1), wherein the ultrasonic power supply is connected with the ultrasonic probe.

Images