CN113827522B

CN113827522B - A cortex Phellodendri extract for cosmetic and its preparation method

Info

Publication number: CN113827522B
Application number: CN202111212078.6A
Authority: CN
Inventors: 朱士强; 李�瑞; 亓云吉
Original assignee: Shandong Huawutang Biotechnology Co ltd
Current assignee: Shandong Huawutang Biotechnology Co ltd
Priority date: 2021-10-18
Filing date: 2021-10-18
Publication date: 2023-03-31
Anticipated expiration: 2041-10-18
Also published as: CN113827522A

Abstract

The invention discloses a preparation method of a phellodendron extract for cosmetics, which is characterized by comprising the following steps: the method comprises the following steps: carrying out enzymolysis treatment on phellodendron amurense to obtain an enzymolysis product; step two: distilling and extracting the phellodendron amurense enzymatic hydrolysate at 85-100 ℃ by using an ethanol solution with the mass concentration of 60-95% to obtain a crude extract; step three: and (3) decoloring and removing impurities from the crude extract, and removing the solvent by rotary evaporation to obtain the phellodendron extract. Compared with the conventional extraction processes such as a traditional water decoction method and the like, the preparation method of the phellodendron extract provided by the invention has the advantages that the obtained phellodendron extract has lower viscosity, shows better antibacterial effect and is more suitable for cosmetic raw materials.

Description

A cortex Phellodendri extract for cosmetic and its preparation method

Technical Field

The application relates to the technical field of cosmetics, in particular to a preparation method of a phellodendron extract for anti-allergy and bacteriostatic cosmetics and the phellodendron extract prepared by the method.

Background

Cortex Phellodendri is the dried bark of phellodendron amurense belonging to Rutaceae. Is obtained by the steps of peeling barks, removing coarse leaves, drying in the sun and the like. Mainly produced in Sichuan, guizhou, hubei, yunnan, etc. The dried bark of phellodendron amurense is used as the medicine part, has bitter taste and cold nature, can treat typhoid fever, yellow body, fever, damp-heat in lower-jiao, leukorrhagia and the like, and has the effects of clearing heat and drying dampness, purging fire and removing steam, detoxifying and treating sore. It is often combined with Zhi Zi, gan Cao, shan Yao and che Qian Zi.

The traditional Chinese medicine extraction method comprises water decoction, soaking, percolation, ultrasonic extraction, etc. Of these, the water decoction method is the most commonly used method. Water is a strong polar solvent, and the extract obtained by the water decoction method contains a large amount of hydrophilic components, such as saccharides, proteins, organic acid salts, etc. These materials can greatly increase the viscosity of the system at high temperatures, and the increased cost of post-processing can limit its use in other applications.

The organic solvent extraction can improve the extraction efficiency and selectivity by selecting different solvents, and the characteristic enables the traditional Chinese medicine extraction to be applied to different fields. The ethanol is one of the most common solvents, has excellent dissolving capacity, strong penetrating power to Chinese herbal medicine cells, short extraction time, less dissolved water-soluble impurities, low toxicity and low price, and can extract specific substances by the ethanol solvents with different concentrations.

The propionibacterium acnes is pathogenic bacteria causing acne, and the propionibacterium acnes can be effectively killed to inhibit the breeding of the acne from the root and finally achieve the effect of removing the acne, so that the bacteriostatic and acne-removing effects of a target object can be effectively measured according to the result of a propionibacterium acnes bacteriostatic experiment. When cells are stimulated by allergens, they release the intragranular substances in large quantities in an exocytic manner, thereby causing allergic reactions. The degranulation experiment is a method for testing specific antibodies in vitro, and can effectively evaluate the antiallergic capability of a target object. In actual research, the applicant finds that the anti-allergy and bacteriostatic ability of the phellodendron extract with higher viscosity obtained by the conventional preparation method is difficult to further improve when the phellodendron extract is applied to cosmetics.

CN102357066A discloses a method for preparing a phellodendron extract, which comprises the steps of firstly treating phellodendron powder with cellulase and then extracting by a water decoction method, however, the method still adopts the water decoction method for extraction, and the viscosity of the system is difficult to reduce.

Therefore, the prior art can also provide the phellodendron extract with lower viscosity and better anti-allergy and bacteriostatic activity.

Disclosure of Invention

In order to solve the above problems, the present invention aims to provide a method for preparing an extract of phellodendron amurense for cosmetics, comprising the steps of:

the method comprises the following steps: performing enzymolysis treatment on phellodendron to obtain phellodendron hydrolysate;

step two: distilling and extracting the phellodendron amurense enzymatic hydrolysate for 2~3 times by using an ethanol solution with the mass concentration of 60-85% at the temperature of 85-100 ℃ to obtain a crude phellodendron amurense extract;

step three: and (3) decoloring and removing impurities from the crude extract, and removing the solvent by rotary evaporation to obtain the phellodendron extract.

Further, in the first step, the step of enzymolysis specifically includes: adding 2-3 times of water based on the mass of the phellodendron amurense into the phellodendron amurense, adding 3% -5% of compound enzyme solution based on the mass of the phellodendron amurense, controlling the pH value to be 4.5-6.5, and stirring and performing enzymolysis for 90-180min at the temperature of 40-45 ℃.

Further, the compound enzyme liquid comprises the following components in an enzyme activity ratio of (5 to 15): (3.5 to 8.5): (0.5-3.5): (1.5-2) cellulase, mannanase, pectinase and protease, wherein the total enzyme activity in the compound enzyme solution is 6-7 ten thousand U/g.

Preferably, the enzyme activity ratio of the cellulase, the mannase, the pectinase and the protease in the compound enzyme solution is (8-10): (5-6): (1.2-1.5): 1.6.

further, the first step further comprises a step of pretreating the golden cypress, wherein the pretreatment comprises the following steps: drying cortex Phellodendri in sunlight for 5-10 days, sieving and filtering stone and mud impurities to obtain strip with length of 3-5 cm and width of 1-2 cm.

Further, in the second step, 600-1200mL of ethanol solution is added to each 100g of the enzymatic hydrolysate prepared from the golden cypress.

Preferably, in the second step, the amount of the phellodendron amurense is 500g, the mass concentration of the ethanol solution is 80%, the amount of the solvent is 4000 mL, the extraction device is a distillation reflux device, the reaction temperature is 95 ℃, and the extraction times are 2 times.

Further, in the third step, the steps of decoloring and removing impurities specifically comprise: adding activated carbon into the crude extract, decolorizing at 55-85 deg.C for 10-12 hr, and vacuum filtering with a filter containing diatomaceous earth and EDTA-2 Na.

Preferably, in the third step, the dosage of the activated carbon is 200 g, the decoloring process is carried out under the condition of a water bath at 60 ℃, the decoloring time is 12h, and the decolored solution is poured into a filtering device while the solution is hot. The kieselguhr in the filtering device is beneficial to further removing impurities, and the disodium ethylene diamine tetraacetate (EDTA-2 Na) is used for chelating and dissociating heavy metal ions, so that clear and transparent filtrate can be obtained after filtering.

Further, the mass ratio of the diatomite to the EDTA-2Na is 100: (1-8).

Further, the method also comprises the step of mixing the phellodendron extract, 1,3-propylene glycol and water to prepare a solution with the concentration of 0.1% -10%, and sterilizing and filling.

Preferably, the sterilization is high-temperature steam sterilization, and the sterilization time is 3-5h.

On the other hand, the application also provides the phellodendron extract prepared by the preparation method, wherein the viscosity of the phellodendron extract at 25 ℃ is 80-90cps.

On the other hand, the application also provides the application of the phellodendron extract in preparing cosmetics, wherein the cosmetics comprise anti-allergy and bacteriostatic cosmetics.

Optionally, the dosage form of the anti-allergic and bacteriostatic cosmetic comprises, but is not limited to, aqua, lotion, cream, essence and gel.

The invention has the following beneficial effects:

1. according to the preparation method of the phellodendron extract, the phellodendron is subjected to enzymolysis treatment by adopting the complex enzyme with a specific enzyme activity ratio, and then the ethanol with a specific mass concentration is used as a solvent for distillation and extraction, so that compared with the conventional extraction processes such as a traditional water decoction method, the obtained phellodendron extract has lower viscosity, shows a better antibacterial effect, and is more suitable for cosmetic raw materials.

2. The phellodendron extract prepared by the preparation method provided by the invention has the advantages of less impurities, high purity, excellent acne-removing, anti-bacteria, anti-allergy and anti-corrosion capabilities, lower contents of protein and polysaccharide substances, better stability, stable existence under the conditions of low temperature, cold and hot circulation, illumination and the like, and can be used as an effective raw material or a natural preservative to be applied to a formula system of cosmetics.

Drawings

The accompanying drawings, which are included to provide a further understanding of the application and are incorporated in and constitute a part of this application, illustrate embodiment(s) of the application and together with the description serve to explain the application and not to limit the application. In the drawings:

FIG. 1 is a micrograph of the degranulation process of the phellodendron amurense extract.

The specific implementation mode is as follows:

in the following description, numerous specific details are set forth by way of examples in order to provide a more thorough understanding of the present invention. It will be apparent, however, to one skilled in the art, that the present invention may be practiced without one or more of these specific details. In other instances, well-known features have not been described in order to avoid obscuring the invention.

The materials and equipment used in the following examples are commercially available, and if not specifically mentioned, the raw material grades in the following examples are all cosmetic grades and are all commercially available.

In addition, the "water" in the present invention includes any available water that can be used in the cosmetic field such as deionized water, distilled water, ion-exchanged water, double distilled water, high purity water, purified water, and the like.

Example 1

The embodiment provides a preparation method of a phellodendron extract for cosmetics, which comprises the following specific steps:

firstly, taking a plurality of phellodendron barks, drying for one week, sieving and filtering impurities such as stones, soil and the like, and weighing the phellodendron barks with the length of 100g being about 6 cm for later use.

Secondly, adding 2 times of water based on the mass of the phellodendron amurense into the phellodendron amurense, and adding 4% of compound enzyme liquid based on the mass of the phellodendron amurense, wherein the total enzyme activity in the compound enzyme liquid is about 6 ten thousand U/g, and the enzyme activity ratio is 8:5:1.5:1.6, regulating the pH value to 5.0 by using oxalic acid, stirring and performing enzymolysis at 42 ℃ for 120min to obtain the phellodendron amurense zymolyte.

Thirdly, preparing 1000 mL ethanol/water solution with the mass concentration of 60 wt%, directly adding the phellodendron amurense zymolyte and the ethanol/water solution into a three-opening beaker together with liquid and residues without filtering, and additionally installing a condensing reflux device. The reactor was placed in a constant temperature water bath with the heating temperature set at 90 ℃. The reaction lasts for 3 h, crude extract of phellodendron is obtained after the reaction is finished, the reaction is repeated once, and the extract obtained by the two reactions is mixed uniformly for later use.

Fourthly, adding 100g activated carbon into the crude phellodendron extract, stirring uniformly, and placing the mixture in a constant-temperature water bath kettle at 80 ℃ for decoloring 12 h. And (3) building a reduced pressure suction filtration device, wherein the filtration device consists of 50 g diatomite and 1 g of EDTA-2Na, and filtering the crude extract of the golden cypress while hot after the decolorization reaction is finished to remove activated carbon and other impurities to obtain a clear and transparent solution.

Fifthly, removing the ethanol/water solvent in the solution by using a rotary evaporator to obtain the extract of 10 g phellodendron amurense.

Sixthly, adding 290 g and 1,3-propylene glycol 700 g into a beaker, uniformly mixing, sterilizing by using high-temperature steam for 4 h, and filling the solution after sterilization is finished, so that the golden cypress extracting solution with the concentration of 10% is obtained.

Example 2

firstly, taking a plurality of phellodendron barks, drying for one week, sieving and filtering impurities such as stones, soil and the like, and weighing 200 g phellodendron barks with the length of about 5 cm for later use.

Thirdly, preparing 2000 mL ethanol/water solution with the mass concentration of 80 wt%, directly adding the phellodendron amurense zymolyte and the ethanol/water solution into a three-opening beaker together with liquid and residues without filtering, and additionally installing a condensing reflux device. The reactor was placed in a constant temperature water bath with the heating temperature set at 90 ℃. The reaction lasts for 2h, crude extract of phellodendron is obtained after the reaction is finished, the reaction is repeated twice, and the extract obtained by the three reactions is mixed uniformly for later use.

Fourthly, adding 100g activated carbon into the crude phellodendron extract, stirring uniformly, and placing the mixture in a constant-temperature water bath kettle at 80 ℃ for decoloring 12 h. And (3) building a reduced pressure suction filtration device, wherein the filtration device is composed of 100g diatomite and 3 g EDTA-2Na, and filtering the crude extract of the golden cypress while hot after the decolorization reaction is finished, removing activated carbon and other impurities, and obtaining a clear and transparent solution.

Fifthly, removing the ethanol/water solvent in the solution by using a rotary evaporator to obtain the extract of 20 g phellodendron amurense.

Sixthly, adding deionized water 580 g and 1,3-propylene glycol 1400 g into a beaker, uniformly mixing, sterilizing by using high-temperature steam for 4 h, and filling the solution after sterilization is finished, so that the golden cypress extracting solution with the concentration of 1% is obtained.

Example 3

firstly, taking a plurality of phellodendron barks, drying for one week, sieving and filtering impurities such as stones, soil and the like, and weighing 400 g phellodendron barks with the length of about 5 cm for later use.

Secondly, adding 2 times of water based on the mass of the phellodendron amurense into the phellodendron amurense, adding 4% of compound enzyme liquid based on the mass of the phellodendron amurense, wherein the total enzyme activity in the compound enzyme liquid is about 6 ten thousand U/g, and the enzyme activity ratio is 8:5:1.5:1.6, regulating the pH value to 5.0 by using oxalic acid, stirring and performing enzymolysis at 42 ℃ for 120min to obtain the phellodendron amurense zymolyte.

And thirdly, preparing an ethanol/water solution with the mass concentration of 4000 mL of 80 wt%, directly adding the phellodendron enzymatic hydrolysate and the liquid and the residues into a three-mouth beaker together with the ethanol/water solution without filtering, and additionally installing a condensing reflux device. The reactor was placed in a constant temperature water bath and the heating temperature was set to 95 ℃. The reaction lasts for 3 h, crude extract of phellodendron is obtained after the reaction is finished, the reaction is repeated once, and the extract obtained by the two reactions is mixed uniformly for later use.

Fourthly, adding 200 g activated carbon into the crude phellodendron extract, stirring uniformly, and placing the mixture in a constant-temperature water bath kettle at 60 ℃ for decoloring 12 h. And (3) building a reduced pressure suction filtration device, wherein the filtration device consists of 100g diatomite and 5 g EDTA-2Na, and filtering the crude extract of the golden cypress while hot after the decolorization reaction is finished, removing activated carbon and other impurities, and obtaining a clear and transparent solution.

Fifthly, removing the ethanol/water solvent in the solution by using a rotary evaporator to obtain 50 g phellodendron extract.

Sixthly, adding deionized water 550 g and 1,3-propylene glycol 1400 g into a beaker, uniformly mixing, sterilizing by using high-temperature steam for 4 h, and filling the solution after sterilization is finished, namely the phellodendron extract with the concentration of 2.5%.

Example 4

firstly, taking a plurality of phellodendron barks, drying for one week, sieving and filtering impurities such as stones, soil and the like, and weighing the phellodendron barks with the length of 500g of about 5 cm for later use.

Thirdly, preparing an ethanol/water solution with the mass concentration of 4000 mL of 80 wt%, directly adding the phellodendron amurense zymolyte and the ethanol/water solution into a three-opening beaker together with liquid and residues without filtering, and additionally installing a condensing reflux device. The reactor was placed in a constant temperature water bath and the heating temperature was set to 95 ℃. The reaction lasts for 3 h, crude extract of phellodendron is obtained after the reaction is finished, the reaction is repeated once, and the extract obtained by the two reactions is mixed uniformly for later use.

Fourthly, adding 200 g activated carbon into the crude phellodendron extract, stirring uniformly, and placing the mixture in a constant-temperature water bath kettle at 60 ℃ for decoloring 12 h. And (3) building a reduced pressure suction filtration device, wherein the filtration device consists of 100g diatomite and 5 g of EDTA-2Na, and filtering the crude extract of the golden cypress while hot after the decolorization reaction is finished to remove activated carbon and other impurities to obtain a clear and transparent solution.

Fifthly, removing the ethanol/water solvent in the solution by using a rotary evaporator to obtain 60 g phellodendron extract.

Sixthly, adding deionized water 540 g and 1,3-propylene glycol 1400 g into a beaker, uniformly mixing, sterilizing by using high-temperature steam for 4 h, and filling the solution after sterilization is finished, namely the phellodendron extract with the concentration of 3%.

Examples 5 to 10

Example 5 and example 6 were prepared in substantially the same manner as example 4, except that the ethanol mass concentration used in the third step was different, as shown in table 1.

Examples 7-10 were prepared in substantially the same manner as in example 4, except that the kind and ratio of the enzyme in the complex enzyme used in the second step were different, as shown in Table 1.

Comparative example 1

This comparative example is substantially the same as the preparation method of example 4, except that extraction is performed using water as an extractant in the third step.

Comparative example 2

This comparative example is about the same as the preparation method of example 4 except that the second step is not performed and distillation extraction is directly performed with an ethanol solution.

The phellodendron extracts obtained in the above examples and comparative examples were tested for viscosity, MIC for the minimum inhibitory concentration of propionibacterium acnes, and storage stability under various environments. Wherein, the test samples adopt the original phellodendron extract without solvent, and are not prepared with 1,3-propylene glycol and water; the viscosity test was performed using a laboratory NDJ-5S viscometer; the test method for the minimum inhibitory concentration MIC of Propionibacterium acnes is described below; the storage time under different environments is 72h. The results are shown in Table 1.

TABLE 1

Examples of the invention

Composite enzyme liquid

Ethanol Concentration of

Viscosity- cps

Minimal concentration of bacteriostatic Degree MIC

Low temperature of-20 deg.C

High temperature of 60 DEG C

Cold and heat cycle

Illumination of light

Practice of Example 1

Cellulase: mannanase: and (3) pectinase: protease enzyme =8：5：1.5：1.6

60%

103

0.625%

Clear, transparent and without precipitate Or precipitate out

A small amount of precipitate is separated out

Has trace amount of precipitation

Clear, bright and no sediment Precipitation of

Practice of Example 2

Cellulase: mannanase: and (3) pectinase: protease enzyme =8：5：1.5：1.6

80%

89

0.025%

Clear and bright without sediment Precipitation out of

Clear, bright and no sediment Precipitation of

Practice of Example 3

Cellulase: mannanase: and (3) pectinase: protease enzyme =8：5：1.5：1.6

80%

85

0.025%

Clear and bright without sediment Precipitation out of

Clear, bright and no sediment Precipitation of

Practice of Example 4

Cellulase: mannanase: and (3) pectinase: protease enzyme =8：5：1.5：1.6

80%

83

0.025%

Clear and bright without sediment Precipitation out of

ClarificationClear and without sinking Precipitation of

Clear, bright and no sediment Precipitation of

Practice of Example 5

Cellulase: mannanase: and (3) pectinase: protease enzyme =8：5：1.5：1.6

85%

59

0.125%

Has trace amount of precipitation

Clear, bright and no sediment Precipitation of

Has trace amount of precipitation

Clear, bright and no sediment Precipitation of

Practice of Example 6

Cellulase: mannanase: and (3) pectinase: protease enzyme =8：5：1.5：1.6

90%

42

0.625%

Has trace amount of precipitation

Clear, bright and no sediment Precipitation of

Has trace amount of precipitation

Clear, bright and no sediment Precipitation of

Practice of Example 7

Cellulase: mannanase: pectinase =8:5: 1.5

80%

112

3.125%

clear and bright without sediment Precipitation out of

Has trace amount of precipitation

Clear, bright and no sediment Precipitation of

Practice of Example 8

Cellulase: mannanase =8:5

80%

131

3.125%

Clear and bright without sediment Precipitation out of

A small amount of precipitate is separated out

Clear, bright and no sediment Precipitation of

Clear, bright and no sediment Is precipitated out

Practice of Example 9

Cellulase: mannanase: and (3) pectinase: protease enzyme =5：8：1.5：1.6

80%

127

3.125%

Clear and bright without sediment Precipitation out of

Has trace amount of precipitation

Clear, bright and no sediment Precipitation of

Practice of Example 10

Cellulase: mannanase: and (3) pectinase: protease enzyme =8：5：2：3

80%

65

3.125%

Has trace amount of precipitation

Clear, bright and no sediment Precipitation of

Comparison of Example 1

Cellulase: mannanase: and (3) pectinase: protease enzyme =8：5：1.5：1.6

0%

286

6.25%

More precipitate is separated out

A small amount of precipitate is separated out

Has trace amount of precipitation

Comparison of Example 2

-

80%

195

6.25%

More precipitate is separated out

A small amount of precipitate is separated out

Has trace amount of precipitation

As can be seen from the data in Table 1, when the obtained phellodendron extract has a viscosity of 80-90cps, it can achieve the best bacteriostatic activity against Propionibacterium acnes, while having good storage stability. In addition, in the preparation method provided by the application, the type, the using amount and the proportion of the complex enzyme and the mass concentration of ethanol during distillation and extraction by using an ethanol aqueous solution have important influences. Of these, example 4 is the most preferred example in the present application.

The phellodendron extract prepared in example 4 was subjected to a propionibacterium acnes bacteriostasis test, and the results are shown in table 2 by using an agar dilution method according to WS/T639-2018 technical requirements for antimicrobial susceptibility tests. Test results show that the phellodendron extract prepared by the method has a good bacteriostatic action on propionibacterium acnes, and the phellodendron extract is preliminarily shown to be added into a cosmetic formula as a raw material with an acne removing effect.

TABLE 2 Propionibacterium acnes bacteriostatic test results

The phellodendron amurense extract prepared in example 4 was subjected to a cell degranulation experiment using mast cells as a cell model, and the detection means was microscopic observation. As shown in fig. 1 and table 3, the numbers of degranulated cells in the NC group were significantly increased compared to the BC group, indicating that the stimulation conditions were effective in this experiment. Compared with the NC group, the number of the degranulated cells of the PC group is obviously reduced, which indicates that the positive control detection is effective, and the result indicates that the prepared phellodendron extract has obvious antiallergic capability.

TABLE 3 Degranulation Rate test results

Sample name	Mean value of degranulation	SD	P-value
				BC (blank control)	0.00 %	0.00 %	/
NC (negative control)	39.09 %	0.57 %	0.000<0.001
				PC (Positive control)	3.85 %	0.64 %	0.000<0.001
0.3125% of cortex Phellodendri extract	31.80 %	0.53 %	0.000<0.001
				Cortex Phellodendri extract-0.1563%	19.93 %	1.55 %	0.000<0.001
0.0781% of phellodendron extract	25.85 %	1.61 %	0.000<0.001

The phellodendron extract prepared in example 4 was subjected to a preservation challenge test, and the growth conditions of five microorganisms, namely staphylococcus aureus, pseudomonas aeruginosa, escherichia coli, candida albicans and aspergillus niger, when the phellodendron extract was left for 28 days were tested, and the results are shown in tables 4 and 5, which indicate that the phellodendron extract has an obvious bactericidal effect on the five microorganisms, and the phellodendron extract meets the class a standard in the preservation efficacy evaluation in comparison with the standard in ISO 11930-2019, and has good preservation performance.

Table 4 corrosion protection challenge experimental results

TABLE 5 evaluation of Corrosion protection effectiveness

Sample name	Basis of detection	Evaluation of Corrosion protection effectiveness
			Cortex Phellodendri extract	ISO 11930 - 2019	Meets the A standard

In summary, the phellodendron amurense plant extract provided by the application adopts a traditional Chinese medicine plant source, and has the advantages of good naturality, no toxic or side effect, mildness and no irritation. And laboratory tests show that enzymolysis and alcohol extraction are combined, so that the effect of extracting the phellodendron extract with high biological activity at a low temperature and a high yield is realized, the obtained product can effectively inhibit the growth of propionibacterium acnes, and meanwhile, the product has good antiallergic capability and antiseptic property, so that a foundation is laid for the application of the product in the field of preparing acne-inhibiting medicines or cosmetics.

The above description is only an example of the present application and is not intended to limit the present application. Various modifications and changes may occur to those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application should be included in the scope of the claims of the present application.

Claims

1. A method for preparing a phellodendron extract for cosmetics, which is characterized by comprising the following steps:

the method comprises the following steps: carrying out enzymolysis treatment on the phellodendron to obtain a phellodendron hydrolysate;

step two: distilling and extracting the phellodendron amurense enzymatic hydrolysate for 2~3 times by using an ethanol solution with the mass concentration of 80% at the temperature of 85-100 ℃ to obtain a phellodendron amurense crude extract;

step three: decolorizing and removing impurities from the crude extract, and removing a solvent by rotary evaporation to obtain the phellodendron extract;

in the first step, the step of enzymolysis specifically comprises: adding 2-3 times of water based on the mass of the phellodendron amurense into the phellodendron amurense, adding 3% -5% of compound enzyme solution based on the mass of the phellodendron amurense, controlling the pH value to be 4.5-6.5, and stirring and performing enzymolysis for 90-180min at the temperature of 40-45 ℃;

the compound enzyme solution comprises the following components in an enzyme activity ratio of (8 to 10): (5~6): (1.2-1.5) 1.6 of cellulase, mannanase, pectinase and protease, wherein the total enzyme activity in the compound enzyme liquid is 6-7 ten thousand U/g.

2. The method of preparing an phellodendron extract for cosmetics according to claim 1, wherein the step one further comprises a step of pretreating phellodendron, the pretreatment comprising: drying cortex Phellodendri in sunlight for 5-10 days, sieving and filtering stone and mud impurities to obtain strip with length of 3-5 cm and width of 1-2 cm.

3. The method of preparing cortex Phellodendri extract for cosmetic use according to claim 1, wherein 600-1200mL of ethanol solution is added to 100g of cortex Phellodendri hydrolysate in step two.

4. The method for preparing the phellodendron amurense extract for cosmetics according to claim 1, wherein the step three of decoloring and removing impurities specifically comprises the steps of: adding activated carbon into the crude extractive solution of cortex Phellodendri, decolorizing at 55-85 deg.C for 10-12h, and vacuum filtering with a filter containing diatomaceous earth and EDTA-2 Na.

5. The method for preparing a phellodendron amurense extract for cosmetics according to claim 4, wherein the mass ratio of the diatomaceous earth to EDTA-2Na is 100: (1-8).

6. The preparation method of the phellodendron extract used for cosmetics according to claim 1, wherein the method further comprises the step of mixing the phellodendron extract with 1,3-propylene glycol and water to prepare a solution with a concentration of 0.1% -10%, and sterilizing and filling.

7. The phellodendron extract obtained by the preparation method according to any one of claims 1 to 6, wherein the viscosity of the phellodendron extract at 25 ℃ is 80 to 90cps.

8. Use of the phellodendron extract according to claim 7 for the preparation of cosmetics, which comprises anti-allergic and bacteriostatic cosmetics.