CN113826901A - High-activity probiotic product with gastrointestinal tract regulating function and preparation method thereof - Google Patents
High-activity probiotic product with gastrointestinal tract regulating function and preparation method thereof Download PDFInfo
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- CN113826901A CN113826901A CN202110996932.6A CN202110996932A CN113826901A CN 113826901 A CN113826901 A CN 113826901A CN 202110996932 A CN202110996932 A CN 202110996932A CN 113826901 A CN113826901 A CN 113826901A
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Classifications
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- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
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- A23L3/3409—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of gases, e.g. fumigation; Compositions or apparatus therefor
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
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- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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Abstract
The invention discloses a high-activity probiotic product with a gastrointestinal tract adjusting function and a preparation method thereof. The high-activity probiotic product with the function of regulating the gastrointestinal tract consists of a gastric acid resistant protective layer, an oxidation resistant layer and a core protective layer. The gastric acid resistant protective layer comprises the following components in percentage by weight: hydroxypropyl starch: glycerol: purifying water: d-mannitol: and (3) the additive is 80-120: 20-40: 60-150: 4.5-9.5: 2.05 to 6.1; the core protective layer comprises the following components in percentage by weight: prebiotics: 1-20 parts of plant dry powder or extract: 1-30: 1-20; the weight ratio of each component of the anti-oxidation layer is as follows: 1-5% of linseed oil: 70-99 parts. The invention also discloses a preparation method of the high-activity probiotic product with the function of regulating the gastrointestinal tract, and the preparation method is simple to operate, low in production cost and suitable for industrial mass production.
Description
Technical Field
The invention belongs to the technical field of probiotics science, and particularly relates to a high-activity probiotic product with a gastrointestinal tract adjusting function and a preparation method thereof.
Background
Many health problems of the human body are directly or indirectly linked to the microbial flora in the intestinal tract. In the digestive system of the human body, the intestine is the largest digestive organ of the human body, and about 30 species of bacteria belonging to more than 500 species inhabit in the human intestine. The probiotics is applied to the treatment of human diseases, plays a role by improving the ecological balance of intestinal flora, and is an active fungus preparation and a metabolite which can improve the health level of human bodies. The intestinal flora coexists with the human body for a long time to form specific microecological balance, help the host digest and absorb nutrient substances in food, metabolize toxic waste generated in the intestinal tract of the host, and generate functional substances such as amino acid, vitamin, short-chain fatty acid and the like which are necessary for the human body for the host. The disturbance of intestinal flora can cause the dysfunction of digestive tract, even one of the main causes of gastric cancer and colorectal cancer. Therefore, maintaining a healthy intestinal flora balance is critical to maintaining the health of the body. The probiotics can regulate the intestinal flora structure of a human body, inhibit colonization of pathogenic bacteria in the intestinal tract, help a host to establish a healthy intestinal mucosa protective layer, enhance the barrier effect of the intestinal tract and enhance the immune system of the host.
Probiotics are a class of active microorganisms that exert their probiotic effect only upon site-directed release after the viable state reaches the gut, thereby producing one or more demonstrable functional health benefits to the host. But products containing probiotics are stressed by environmental factors such as light, oxygen, temperature, moisture, pressure and the like in the processes of processing, storing and transporting and are easy to lose activity, so that the biological activity is reduced, and the quality of the products is unstable; and in the process of taking the probiotic product, people are treated by digestive juice such as gastric acid, bile salt and the like, so that enough viable bacteria can not reach the intestinal tract, the due probiotic effect of the probiotic product is lost, and the result that the actual release effect in the intestinal tract of a host can not meet the requirement is caused.
In addition, the metabolite of one kind of bacteria may stimulate the growth and metabolism of another kind of bacteria, so as to produce the product which can not be obtained by single-bacteria fermentation and high viable count, the mixed culture of probiotics to produce composite probiotic preparation to play the function of multiple probiotics is a great trend at present, although some multi-strain combined preparations are also appeared in the market recently, the combined preparations are mostly prepared by single-bacteria culture and combination, the adding amount of each bacteria is determined according to the adding amount of each bacteriaThe probiotic bacteria are unexpected and lack certain scientificity, and antagonism and other phenomena sometimes occur among different bacterial strains, so that the probiotic efficiency is influenced. Therefore, the product is prepared by mixed culture of the probiotic powder raw materials, which not only can overcome the antagonistic phenomenon between strains in the combined preparation product, but also is beneficial to playing the coordination and complementation role of the two bacteria (for example, lactobacillus acidophilus can consume the oxygen remained in the gastrointestinal tract, so as to provide an anaerobic environment for the growth of bifidobacterium and promote the growth of bifidobacterium, and vitamin B generated by the bifidobacterium in the growth process1、B6、B12Folic acid, etc. is favorable to the growth of acidophilic lactobacillus and has raised probiotics effect.
At present, a high-activity probiotic product with the function of regulating gastrointestinal tract does not exist, so that people can accept the probiotic product more conveniently and more easily, and the probiotics are reasonably proportioned and have synergistic effect, so that the microbial ecology of the gastrointestinal tract is balanced, the function of the gastrointestinal tract is regulated, and the functional disorder of the digestive tract is effectively relieved. The main challenge of all probiotic products is to improve the activity of the bacteria and ensure the stability of the products, thereby prolonging the shelf life of the products.
Disclosure of Invention
In order to overcome the technical defects, the invention aims to provide a high-activity probiotic product, and through researches on a product formula and a production and preparation process thereof, the survival rate of probiotics is effectively improved, the stability of the product is ensured, and meanwhile, the production cost can be reduced. According to the invention, various probiotic powder raw materials are compounded, mixed and cultured under appropriate conditions, and the screened compound probiotic strain with the function of regulating gastrointestinal tract is placed in a sterile environment at the temperature of 18 ℃ below zero for pre-freezing preservation so as to preserve the high activity of the compound probiotic; by adopting a three-layer embedding technology, stress of digestive juice such as gastric acid, choline and the like can be resisted, and the activity of probiotics can be improved, so that the probiotics effect can be exerted in the intestinal tract, and the quality guarantee period of the product is prolonged; the antioxidant layer is added with linseed oil to resist bile damage, has an antioxidant effect, blocks oxygen and moisture contact, and has better wrapping property; the core protective layer consists of oil-soluble compound probiotics and prebiotics, wherein high-quality prebiotics are added to promote the growth of the probiotics; the gastric acid-resistant protective layer is made of plant starch, and is not easy to cause cross-linking reaction of contents due to chemical inertia, so that the survival rate of probiotics can be effectively improved.
In order to solve the problems, the invention is realized according to the following technical scheme:
a high-activity probiotic product with the function of regulating gastrointestinal tract is characterized by comprising a gastric acid-resistant protective layer, an oxidation-resistant layer and a core protective layer;
the gastric acid resistant protective layer comprises hydroxypropyl starch, glycerol, purified water, D-mannitol and an additive, wherein the weight ratio of the hydroxypropyl starch: glycerol: purifying water: d-mannitol: additive = 80-120: 20-40: 60-150: 4.5-9.5: 2.05 to 6.1;
the core protective layer comprises compound probiotic powder, prebiotics and plant dry powder or extract, and the weight ratio of the components is as follows: prebiotics: dry plant powder or extract = 1-20: 1-20: 1-10;
the antioxidation layer comprises mono-diglycerol fatty acid ester and linseed oil, and the weight ratio of the components is as follows: linseed oil = 1-5: 70-99 parts.
Further, the additive comprises edible pigment, sweetener and fruit-flavored essence, wherein the weight ratio of the edible pigment to the fruit-flavored essence is as follows: a sweetening agent: fruity flavor = 0.04-0.35: 0.01-0.25: 2 to 5.5;
the edible pigment is one or more of titanium dioxide, lemon yellow, brilliant blue, indigo, carmine, amaranth, allura red, sunset yellow, riboflavin, carotene and anthocyanin;
the sweetening agent is one or a combination of more of sucralose, glucose, stevioside and ammonium glycyrrhizinate;
the fruity essence is one or a combination of apple essence, strawberry essence, blueberry essence, orange essence, lemon essence and cherry essence.
Further, the compound probiotic powder comprises one or more of bifidobacterium lactis BLa80 freeze-dried powder, lactobacillus plantarum N13 freeze-dried powder, lactobacillus rhamnosus LRa05 freeze-dried powder, lactobacillus acidophilus LA85 freeze-dried powder, pediococcus acidilactici CCFM7902 freeze-dried powder, bifidobacterium breve BBr60 freeze-dried powder and bifidobacterium longum BL21 freeze-dried powder.
Further, the ratio of the mass parts of the bifidobacterium lactis BLA80 freeze-dried powder, the lactobacillus plantarum N13 freeze-dried powder, the lactobacillus rhamnosus LRa05 freeze-dried powder, the lactobacillus acidophilus LA85 freeze-dried powder, the pediococcus acidilactici CCFM7902 freeze-dried powder, the bifidobacterium breve BBr60 freeze-dried powder and the bifidobacterium longum BL21 freeze-dried powder is as follows: (1-5): (1-5): (1-5): (1-5): (1-5): (1-5): (1-5).
Further, the prebiotics comprise one or a combination of more of inulin, xylo-oligosaccharide, isomalto-oligosaccharide, fructo-oligosaccharide and stachyose.
Further, the plant dry powder or extract is selected from one or more of hawthorn powder, yam powder, tuckahoe powder, coix seed powder, gordon euryale seed powder and orange peel extract.
Further, a preparation method of the high-activity probiotic product with the function of regulating the gastrointestinal tract comprises the following steps:
step 1, nitrogen filling: during the preparation of raw materials and the preparation of products, introducing inert gas nitrogen in advance to remove oxygen in production equipment, and preparing probiotic products under the protection of the nitrogen;
step 2, preparing and pre-freezing a core protective layer: introducing nitrogen into the bottom layer of the fermentation tank, weighing probiotic bacteria powder and isomaltooligosaccharide according to the formula amount, inoculating the probiotic bacteria powder and isomaltooligosaccharide into the fermentation tank, standing and culturing at 37 ℃ for 20-30 hours, and then freezing and centrifuging at the rotation speed of 8000r/min at 4 ℃ for 30 min; collecting thallus, adding into prebiotics, plant dry powder or extract, mixing, pre-cooling in-18 deg.C sterile environment, and storing at low temperature;
step 3, preparing an anti-oxidation layer: under the protection of nitrogen, dissolving the pre-frozen powder in a formula amount of mono-diglycerol fatty acid ester and linseed oil, controlling an aseptic environment, stirring for 30-40 min at a relative humidity of 15-35% and a temperature of 15-30 ℃ to obtain a mixed solution, and temporarily storing the mixed solution for later use;
step 4, preparing a gastric acid resistant protective layer: adding purified water into a gelatin melting tank according to the formula amount, raising the temperature to 75-85 ℃, adding glycerol, hydroxypropyl starch, D-mannitol, edible pigment, sweetening agent and fruity essence according to the formula amount, filling nitrogen into the gelatin melting tank for nitrogen protection, and continuously preserving heat and stirring to completely dissolve the hydroxypropyl starch; starting a vacuum pump to carry out vacuum pumping and degassing, wherein the vacuum stirring time is about 30-50 min, stopping stirring after degassing is finished, and closing the vacuum pump; sieving the glue solution with a 100-mesh sieve, placing in a heat-preserving container, and standing for 6-12 h;
and step 5, pelleting, forming, drying, polishing and packaging: under the protection of nitrogen, conveying the prepared mixed raw material of the anti-oxidation layer and the core protective layer to a pelleting press, adjusting the loading and the thickness of the rubber, quantitatively pressing and forming the contents, and then drying, polishing and packaging the contents; controlling the temperature of a spray body of the pelleting machine to be 15-30 ℃; the drying time is 10-35 h, the drying temperature is 15-30 ℃, and the relative humidity is 15% -35%.
The invention has the beneficial effects that:
(1) the invention creatively discovers that the screened compound probiotic preparation strain is reasonable in proportion, stable in performance and obvious in effect, and has a synergistic effect and a mixed culture under appropriate conditions after various probiotic powder raw materials are compounded. And the compound probiotics is pre-frozen in a sterile environment at the temperature of-18 ℃, so that the high activity of the compound probiotics is favorably preserved.
(2) The high-activity probiotic product provided by the invention adopts a three-layer embedding technology, can resist the damage of gastric acid and bile, and can keep the activity of the probiotics even after the action of gastric juice and bile salt, so that the probiotics can reach the intestinal tract to play the probiotic effect.
(3) According to the high-activity probiotic product, the gastric acid-resistant protective layer is made of plant starch, and is chemically inert, so that the cross-linking reaction with contents is not easy to occur, and the gastric acid is not easy to dissolve, and therefore, the survival rate of probiotics can be effectively improved. The antioxidant layer is added with linseed oil to resist bile damage, has an antioxidant effect, blocks oxygen and moisture contact, and has better wrapping property; the core protective layer is formed by adding oil-soluble compound probiotics into high-quality prebiotics, and can promote mass propagation of the probiotics.
(4) According to the preparation method of the high-activity probiotic product, provided by the invention, in the processes of raw material preparation and product preparation, inert gas nitrogen is introduced in advance to remove oxygen in production equipment, and the preparation of the probiotic product is carried out under the protection of the nitrogen, so that the oxygen isolation effect is facilitated, and the quality guarantee period of the high-activity probiotic is prolonged.
(5) According to the preparation method of the high-activity probiotic product, the temperature of the environment in which the probiotics are contacted is controlled to be 15-30 ℃ in the production process, and the prepared probiotic product is safe, free of toxic and side effects, convenient to carry and take, simple to operate, low in production cost and suitable for industrial mass production.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
Step 1, selecting the following raw materials in parts by weight: 95 parts of hydroxypropyl starch, 30 parts of glycerol, 100 parts of purified water, 5.5 parts of D-mannitol, 0.1 part of sucralose, 3.5 parts of lemon essence, compound probiotic powder (bifidobacterium lactis BLA80 freeze-dried powder, lactobacillus plantarum N13 freeze-dried powder, lactobacillus rhamnosus LRa05 freeze-dried powder, lactobacillus acidophilus LA85 freeze-dried powder, pediococcus acidilactici CCFM7902 freeze-dried powder, bifidobacterium breve BBr60 freeze-dried powder, bifidobacterium longum BL21 freeze-dried powder 1:3:2:1: 2) 10 parts, 3 parts of mono-diglycerol fatty acid ester, 80 parts of linseed oil, 5 parts of xylo-oligosaccharide, 5 parts of stachyose, 5 parts of hawthorn powder and 0.1 part of carotene.
Step 2, nitrogen filling: during the preparation of raw materials and the preparation of products, introducing inert gas nitrogen in advance to remove oxygen in production equipment, and preparing probiotic products under the protection of the nitrogen;
step 3, preparing and pre-freezing a core protective layer: inoculating compound probiotic powder (Bifidobacterium lactis BLA80 lyophilized powder, Lactobacillus plantarum N13 lyophilized powder, Lactobacillus rhamnosus LRa05 lyophilized powder, Lactobacillus acidophilus LA85 lyophilized powder, Pediococcus acidilactici CCFM7902 lyophilized powder, Bifidobacterium breve BBr60 lyophilized powder, Bifidobacterium longum BL21 lyophilized powder 1:3:2:1:3:1: 2) into a fermentation tank, standing and culturing at 37 ℃ for 24 hours, and then carrying out refrigerated centrifugation at 4 ℃ and 8000r/min for 30 min. Collecting thallus, adding into xylooligosaccharide, stachyose and fructus crataegi powder, mixing, pre-cooling in-18 deg.C sterile environment, and storing at low temperature;
step 4, preparing an anti-oxidation layer: under the protection of nitrogen, dissolving the pre-frozen powder in 3 parts of mono-diglycerol fatty acid ester and 80 parts of linseed oil, stirring for 30min in an aseptic environment with the relative humidity of 20% and the temperature of 28 ℃, obtaining a mixed solution, and temporarily storing the mixed solution for later use;
step 5, preparing a gastric acid resistant protective layer: adding 100 parts of purified water into a gelatin melting tank, preheating to 75-85 ℃, adding 95 parts of hydroxypropyl starch, 30 parts of glycerol, 5.5 parts of D-mannitol, 0.1 part of carotene, 0.1 part of sucralose and 3.5 parts of lemon essence into the gelatin melting tank, filling nitrogen into the gelatin melting tank for nitrogen protection, and continuing to preserve heat and stir to completely dissolve the hydroxypropyl starch; starting a vacuum pump to carry out vacuum pumping and degassing, wherein the vacuum stirring time is about 35min, stopping stirring after degassing is finished, and closing the vacuum pump; sieving the glue solution with a 100-mesh sieve, placing the glue solution in a heat-preserving container, and standing for 10 hours for later use;
step 6, pelleting, forming, drying, polishing and packaging: under the protection of nitrogen, conveying the prepared mixed raw material of the anti-oxidation layer and the core protective layer to a pelleting press, adjusting the loading and the thickness of the rubber, quantitatively pressing and forming the contents, and then drying, polishing and packaging the contents; controlling the temperature of a spray body of the pelleting machine to be 28 ℃; the drying time is 20h, the drying temperature is 25 ℃, and the relative humidity is 20%.
Example 2
Step 1, selecting the following raw materials in parts by weight: 85 parts of hydroxypropyl starch, 26 parts of glycerol, 80 parts of purified water, 5 parts of D-mannitol, 0.1 part of stevioside, 3.5 parts of apple essence, compound probiotic powder (bifidobacterium lactis BLA80 freeze-dried powder, lactobacillus plantarum N13 freeze-dried powder, lactobacillus rhamnosus LRa05 freeze-dried powder, lactobacillus acidophilus LA85 freeze-dried powder, pediococcus acidilactici CCFM7902 freeze-dried powder, bifidobacterium breve BBr60 freeze-dried powder, bifidobacterium longum BL21 freeze-dried powder 2:2:4:1:2:3: 2) 10 parts, 5 parts of mono-diglycerol fatty acid ester, 90 parts of linseed oil, 5 parts of fructo-oligosaccharide, 5 parts of stachyose, 5 parts of yam powder and 0.1 part of anthocyanin.
Step 2, nitrogen filling: during the preparation of raw materials and the preparation of products, introducing inert gas nitrogen in advance to remove oxygen in production equipment, and preparing probiotic products under the protection of the nitrogen;
step 3, preparing and pre-freezing a core protective layer: inoculating compound probiotic powder (Bifidobacterium lactis BLA80 lyophilized powder, Lactobacillus plantarum N13 lyophilized powder, Lactobacillus rhamnosus LRa05 lyophilized powder, Lactobacillus acidophilus LA85 lyophilized powder, Pediococcus acidilactici CCFM7902 lyophilized powder, Bifidobacterium breve BBr60 lyophilized powder, Bifidobacterium longum BL21 lyophilized powder 2:2:4:1:2:3: 2) into a fermentation tank, standing and culturing at 37 ℃ for 24 hours, and then carrying out refrigerated centrifugation at 4 ℃ and 8000r/min for 30 min. Collecting thallus, adding into xylooligosaccharide, stachyose and rhizoma Dioscoreae powder, mixing, pre-cooling in-18 deg.C sterile environment, and storing at low temperature;
step 4, preparing an anti-oxidation layer: under the protection of nitrogen, dissolving the pre-frozen powder in 5 parts of mono-diglycerol fatty acid ester and 90 parts of linseed oil, stirring for 35min under the sterile environment with the relative humidity of 25% and the temperature of 25 ℃, obtaining a mixed solution, and temporarily storing the mixed solution for later use;
step 5, preparing a gastric acid resistant protective layer: adding 80 parts of purified water into a gelatin melting tank, preheating to 75-85 ℃, adding 85 parts of hydroxypropyl starch, 26 parts of glycerol, 5 parts of D-mannitol, 0.1 part of anthocyanin, 0.1 part of stevioside and 3.5 parts of apple essence into the gelatin melting tank, filling nitrogen into the gelatin melting tank for nitrogen protection, and continuing to preserve heat and stir to completely dissolve the hydroxypropyl starch; starting a vacuum pump to carry out vacuum pumping and degassing, wherein the vacuum stirring time is about 40min, stopping stirring after degassing is finished, and closing the vacuum pump; sieving the glue solution with a 100-mesh sieve, placing the glue solution in a heat-preserving container, and standing for 12h for later use;
step 6, pelleting, forming, drying, polishing and packaging: under the protection of nitrogen, conveying the prepared mixed raw material of the anti-oxidation layer and the core protective layer to a pelleting press, adjusting the loading and the thickness of the rubber, quantitatively pressing and forming the contents, and then drying, polishing and packaging the contents; controlling the temperature of a spray body of the pelleting machine to be 30 ℃; the drying time is 25h, the drying temperature is 20 ℃, and the relative humidity is 25%.
Comparative example 1
Step 1, selecting the following raw materials in parts by weight: 95 parts of hydroxypropyl starch, 30 parts of glycerol, 100 parts of purified water, 5.5 parts of D-mannitol, 0.1 part of sucralose, 3.5 parts of lemon essence, compound probiotic powder (bifidobacterium lactis BLA80 freeze-dried powder, lactobacillus plantarum N13 freeze-dried powder, lactobacillus rhamnosus LRa05 freeze-dried powder, lactobacillus acidophilus LA85 freeze-dried powder, pediococcus acidilactici CCFM7902 freeze-dried powder, bifidobacterium breve BBr60 freeze-dried powder, bifidobacterium longum BL21 freeze-dried powder 1:3:2:1: 2) 10 parts, 3 parts of mono-diglycerol fatty acid ester, 80 parts of linseed oil, 5 parts of hawthorn powder and 0.1 part of carotene.
Step 2, nitrogen filling: during the preparation of raw materials and the preparation of products, introducing inert gas nitrogen in advance to remove oxygen in production equipment, and preparing probiotic products under the protection of the nitrogen;
step 3, preparing and pre-freezing a core protective layer: inoculating compound probiotic powder (Bifidobacterium lactis BLA80 lyophilized powder, Lactobacillus plantarum N13 lyophilized powder, Lactobacillus rhamnosus LRa05 lyophilized powder, Lactobacillus acidophilus LA85 lyophilized powder, Pediococcus acidilactici CCFM7902 lyophilized powder, Bifidobacterium breve BBr60 lyophilized powder, Bifidobacterium longum BL21 lyophilized powder 1:3:2:1:3:1: 2) into a fermentation tank, standing and culturing at 37 ℃ for 24 hours, and then carrying out refrigerated centrifugation at 4 ℃ and 8000r/min for 30 min. Collecting thallus, adding into fructus crataegi powder, mixing, pre-cooling at-18 deg.C in sterile environment, and storing at low temperature;
step 4, preparing an anti-oxidation layer: under the protection of nitrogen, dissolving the pre-frozen powder in 3 parts of mono-diglycerol fatty acid ester and 80 parts of linseed oil, stirring for 30min in an aseptic environment with the relative humidity of 20% and the temperature of 28 ℃, obtaining a mixed solution, and temporarily storing the mixed solution for later use;
step 5, preparing a gastric acid resistant protective layer: adding 100 parts of purified water into a gelatin melting tank, preheating to 75-85 ℃, adding 95 parts of hydroxypropyl starch, 30 parts of glycerol, 5.5 parts of D-mannitol, 0.1 part of carotene, 0.1 part of sucralose and 3.5 parts of lemon essence into the gelatin melting tank, filling nitrogen into the gelatin melting tank for nitrogen protection, and continuing to preserve heat and stir to completely dissolve the hydroxypropyl starch; starting a vacuum pump to carry out vacuum pumping and degassing, wherein the vacuum stirring time is about 35min, stopping stirring after degassing is finished, and closing the vacuum pump; sieving the glue solution with a 100-mesh sieve, placing the glue solution in a heat-preserving container, and standing for 10 hours for later use;
step 6, pelleting, forming, drying, polishing and packaging: under the protection of nitrogen, conveying the prepared mixed raw material of the anti-oxidation layer and the core protective layer to a pelleting press, adjusting the loading and the thickness of the rubber, quantitatively pressing and forming the contents, and then drying, polishing and packaging the contents; controlling the temperature of a spray body of the pelleting machine to be 28 ℃; the drying time is 20h, the drying temperature is 25 ℃, and the relative humidity is 20%.
Comparative example 2
Step 1, selecting the following raw materials in parts by weight: 85 parts of hydroxypropyl starch, 26 parts of glycerol, 80 parts of purified water, 5 parts of D-mannitol, 0.1 part of stevioside, 3.5 parts of apple essence, compound probiotic powder (bifidobacterium lactis BLA80 freeze-dried powder, lactobacillus plantarum N13 freeze-dried powder, lactobacillus rhamnosus LRa05 freeze-dried powder, lactobacillus acidophilus LA85 freeze-dried powder, pediococcus acidilactici CCFM7902 freeze-dried powder, bifidobacterium breve BBr60 freeze-dried powder, bifidobacterium longum BL21 freeze-dried powder 2:2:4:1:2:3: 2) 10 parts, 5 parts of mono-diglycerol fatty acid ester, 90 parts of linseed oil, 5 parts of fructo-oligosaccharide, 5 parts of stachyose, 5 parts of yam powder and 0.1 part of anthocyanin.
Step 2, nitrogen filling: during the preparation of raw materials and the preparation of products, introducing inert gas nitrogen in advance to remove oxygen in production equipment, and preparing probiotic products under the protection of the nitrogen;
step 3, preparing and pre-freezing a core protective layer: inoculating compound probiotic powder (Bifidobacterium lactis BLA80 lyophilized powder, Lactobacillus plantarum N13 lyophilized powder, Lactobacillus rhamnosus LRa05 lyophilized powder, Lactobacillus acidophilus LA85 lyophilized powder, Pediococcus acidilactici CCFM7902 lyophilized powder, Bifidobacterium breve BBr60 lyophilized powder, Bifidobacterium longum BL21 lyophilized powder 2:2:4:1:2:3: 2) into a fermentation tank, standing and culturing at 37 ℃ for 24 hours, and then carrying out refrigerated centrifugation at 4 ℃ and 8000r/min for 30 min. Collecting thallus, adding into xylooligosaccharide, stachyose and rhizoma Dioscoreae powder, mixing, pre-cooling in-18 deg.C sterile environment, and storing at low temperature;
step 4, preparing an anti-oxidation layer: under the protection of nitrogen, dissolving the pre-frozen powder in 5 parts of mono-diglycerol fatty acid ester and 90 parts of linseed oil, stirring for 35min under the sterile environment with the relative humidity of 25% and the temperature of 25 ℃, obtaining a mixed solution, and temporarily storing the mixed solution for later use;
step 5, preparing a gastric acid resistant protective layer: adding 80 parts of purified water into a gelatin melting tank, preheating to 75-85 ℃, adding 85 parts of hydroxypropyl starch, 26 parts of glycerol, 5 parts of D-mannitol, 0.1 part of anthocyanin, 0.1 part of stevioside and 3.5 parts of apple essence into the gelatin melting tank, filling nitrogen into the gelatin melting tank for nitrogen protection, and continuing to preserve heat and stir to completely dissolve the hydroxypropyl starch; starting a vacuum pump to carry out vacuum pumping and degassing, wherein the vacuum stirring time is about 40min, stopping stirring after degassing is finished, and closing the vacuum pump; sieving the glue solution with a 100-mesh sieve, placing the glue solution in a heat-preserving container, and standing for 12h for later use;
step 6, pelleting, forming, drying, polishing and packaging: under the protection of nitrogen, conveying the prepared mixed raw material of the anti-oxidation layer and the core protective layer to a pelleting press, adjusting the loading and the thickness of the rubber, quantitatively pressing and forming the contents, and then drying, polishing and packaging the contents; controlling the temperature of a spray body of the pelleting machine to be 30 ℃; the drying time is 25h, the drying temperature is 40 ℃, and the relative humidity is 25%.
Test example 1 gastric acid resistance test of highly active probiotic products
(1) Preparing artificial stomach simulating liquid: taking 16.4 mL (0.1 mol/L) of dilute hydrochloric acid, adding about 800 mL of water and 10g of pepsin, uniformly stirring, adding water to a constant volume of 1000 mL, and filtering and sterilizing by using a sterile microporous filter membrane with the diameter of 0.2 mu m for later use.
(2) Determining the survival rate of the probiotics in the product: the products prepared in the examples and the comparative examples are respectively placed in a triangular flask, then 50mL of artificial gastric juice with the temperature of 37 +/-1 ℃ is added, and the mixture is uniformly mixed and then placed in a constant temperature shaking table. The speed and temperature of the shaker were set at 180r/min, 37. + -. 1 ℃. Shaking is carried out. Sampling is carried out at 0min, 60 min, 90 min, 120 min and 180 min respectively in the shaking process. The samples taken out were subjected to plate counting to determine the viable cell count. Each set of samples was averaged in triplicate. And finally, obtaining the number of the living bacteria in each sample, wherein the survival rate = A/B multiplied by 100%, A is the number of the living bacteria in the sample after the artificial gastric juice treatment, and B is the number of the living bacteria in the sample of the example 1 before the artificial gastric juice treatment.
And (3) respectively adding the high-activity probiotic product obtained in the embodiment 1-2 and naked bacteria corresponding to the oil-soluble probiotic powder into an artificial stomach simulation solution for culturing. The survival in gastric acid was tested according to the above method. The results of the experiment are shown in table 1.
As can be seen from Table 1, under a simulated gastric acid environment, the survival rate of the high-activity probiotic products obtained in examples 1-2 is reduced within 2 hours, wherein the mixed bacteria of the examples are almost completely dead after 2 hours. This shows that the probiotics alone hardly survive in the stomach for 2h, but the survival rate of the probiotics embedded by the plant starch glue in gastric acid can reach more than 70% after 2h, because the plant starch glue contained in the gastric acid resistant layer begins to dissolve in the gastric acid after 2h, the probiotics are strongly protected. In addition, as can be seen from the survival rates of the artificial gastric juice treated for 0min in the example 1 and the comparative examples 1-2, the activity of the probiotic product without prebiotics is slightly reduced, and the activity of the probiotic product is obviously reduced when the drying temperature is set to 40 ℃.
Test example 2 stability test of probiotic products
The high activity probiotic products obtained in examples 1-2 and comparative example 1 and a commercial probiotic powder were subjected to a long-term stability test, and stored at room temperature, and the number of viable probiotic bacteria was measured at 0 month, 1 month, 2 months, 3 months, and 6 months, respectively, and finally the number of viable probiotic bacteria in the sample whose survival rate = C/0 month was obtained × 100%, and the experimental results are shown in table 2.
TABLE 2 probiotic product stability test results
As can be seen from table 2, the probiotic survival rate in the samples decreased with the passage of time under room temperature storage conditions, and after 6 months the probiotic survival rate was significantly decreased in comparative example 1 and the commercial product samples.
Claims (7)
1. The high-activity probiotic product with the function of regulating the gastrointestinal tract is characterized by comprising a gastric acid resistant protective layer, an oxidation resistant layer and a core protective layer;
the gastric acid resistant protective layer comprises hydroxypropyl starch, glycerol, purified water, D-mannitol and an additive, wherein the weight ratio of the hydroxypropyl starch: glycerol: purifying water: d-mannitol: additive = 80-120: 20-40: 60-150: 4.5-9.5: 2.05 to 6.1;
the core protective layer comprises compound probiotic powder, prebiotics and plant dry powder or extract, and the weight ratio of the components is as follows: prebiotics: dry plant powder or extract = 1-20: 1-20: 1-10;
the antioxidation layer comprises mono-diglycerol fatty acid ester and linseed oil, and the weight ratio of the components is as follows: linseed oil = 1-5: 70-99 parts.
2. The high-activity probiotic product with the function of regulating the gastrointestinal tract according to claim 1, wherein the additive comprises food color, sweetener and fruity essence, and the weight ratio of the food color: a sweetening agent: fruity flavor = 0.04-0.35: 0.01-0.25: 2 to 5.5;
the edible pigment is one or more of titanium dioxide, lemon yellow, brilliant blue, indigo, carmine, amaranth, allura red, sunset yellow, riboflavin, carotene and anthocyanin;
the sweetening agent is one or a combination of more of sucralose, glucose, stevioside and ammonium glycyrrhizinate;
the fruity essence is one or a combination of apple essence, strawberry essence, blueberry essence, orange essence, lemon essence and cherry essence.
3. The high-activity probiotic product with the function of regulating the gastrointestinal tract of claim 1, wherein the compound probiotic powder comprises one or more of bifidobacterium lactis BLA80 freeze-dried powder, lactobacillus plantarum N13 freeze-dried powder, lactobacillus rhamnosus LRa05 freeze-dried powder, lactobacillus acidophilus LA85 freeze-dried powder, pediococcus acidilactici CCFM7902 freeze-dried powder, bifidobacterium breve BBr60 freeze-dried powder and bifidobacterium longum BL21 freeze-dried powder.
4. The high-activity probiotic product with the function of regulating the gastrointestinal tract of claim 3, wherein the ratio of the mass parts of the freeze-dried powder of bifidobacterium lactis BLA80, the freeze-dried powder of lactobacillus plantarum N13, the freeze-dried powder of lactobacillus rhamnosus LRa05, the freeze-dried powder of lactobacillus acidophilus LA85, the freeze-dried powder of pediococcus acidilactici CCFM7902, the freeze-dried powder of bifidobacterium breve BBr60 and the freeze-dried powder of bifidobacterium longum BL21 is as follows: (1-5): (1-5): (1-5): (1-5): (1-5): (1-5): (1-5).
5. The high-activity probiotic product with the function of regulating the gastrointestinal tract according to claim 1, wherein the prebiotics comprise one or a combination of inulin, xylo-oligosaccharide, isomalto-oligosaccharide, fructo-oligosaccharide and stachyose.
6. The highly active probiotic product with the function of regulating the gastrointestinal tract according to claim 1, wherein the plant dried powder or extract is selected from one or more of hawthorn powder, yam powder, tuckahoe powder, coix seed powder, gordon euryale seed powder and orange peel extract.
7. The highly active probiotic product with the function of regulating the gastrointestinal tract according to claims 1 to 5, characterized in that the preparation method comprises the following steps:
step 1, nitrogen filling: during the preparation of raw materials and the preparation of products, introducing inert gas nitrogen in advance to remove oxygen in production equipment, and preparing probiotic products under the protection of the nitrogen;
step 2, preparing and pre-freezing a core protective layer: introducing nitrogen into the bottom layer of the fermentation tank, weighing probiotic bacteria powder and isomaltooligosaccharide according to the formula amount, inoculating the probiotic bacteria powder and isomaltooligosaccharide into the fermentation tank, standing and culturing at 37 ℃ for 20-30 hours, and then freezing and centrifuging at the rotation speed of 8000r/min at 4 ℃ for 30 min; collecting thallus, adding into prebiotics, plant dry powder or extract, mixing, pre-cooling in-18 deg.C sterile environment, and storing at low temperature;
step 3, preparing an anti-oxidation layer: under the protection of nitrogen, dissolving the pre-frozen powder in a formula amount of mono-diglycerol fatty acid ester and linseed oil, controlling an aseptic environment, stirring for 30-40 min at a relative humidity of 15-35% and a temperature of 15-30 ℃ to obtain a mixed solution, and temporarily storing the mixed solution for later use;
step 4, preparing a gastric acid resistant protective layer: adding purified water into a gelatin melting tank according to the formula amount, raising the temperature to 75-85 ℃, adding glycerol, hydroxypropyl starch, D-mannitol, edible pigment, sweetening agent and fruity essence according to the formula amount, filling nitrogen into the gelatin melting tank for nitrogen protection, and continuously preserving heat and stirring to completely dissolve the hydroxypropyl starch; starting a vacuum pump to carry out vacuum pumping and degassing, wherein the vacuum stirring time is about 30-50 min, stopping stirring after degassing is finished, and closing the vacuum pump; sieving the glue solution with a 100-mesh sieve, placing in a heat-preserving container, and standing for 6-12 h;
step 5, pelleting, forming, drying, polishing and packaging: under the protection of nitrogen, conveying the prepared mixed raw material of the anti-oxidation layer and the core protective layer to a pelleting press, adjusting the loading and the thickness of the rubber, quantitatively pressing and forming the contents, and then drying, polishing and packaging the contents; controlling the temperature of a spray body of the pelleting machine to be 15-30 ℃; the drying time is 10-35 h, the drying temperature is 15-30 ℃, and the relative humidity is 15% -35%.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114794485A (en) * | 2022-03-28 | 2022-07-29 | 上海启立生物科技有限公司 | A nutritional product for integrally regulating intestinal tract and immune health |
| CN116135047A (en) * | 2023-03-14 | 2023-05-19 | 河北新希望天香乳业有限公司 | Pure cereal probiotic composition and preparation method thereof |
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| CN103893772A (en) * | 2014-04-21 | 2014-07-02 | 湖南同泰胶囊有限公司 | Hydroxypropyl starch empty capsules and preparation process thereof |
| CN110200186A (en) * | 2019-07-15 | 2019-09-06 | 无限极（中国）有限公司 | A kind of probiotics solid beverage and preparation method thereof |
| CN112088967A (en) * | 2020-09-24 | 2020-12-18 | 湖南素囊健康科技有限公司 | Probiotic gel candy suitable for infants and preparation method thereof |
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| CN103893772A (en) * | 2014-04-21 | 2014-07-02 | 湖南同泰胶囊有限公司 | Hydroxypropyl starch empty capsules and preparation process thereof |
| CN110200186A (en) * | 2019-07-15 | 2019-09-06 | 无限极（中国）有限公司 | A kind of probiotics solid beverage and preparation method thereof |
| CN112088967A (en) * | 2020-09-24 | 2020-12-18 | 湖南素囊健康科技有限公司 | Probiotic gel candy suitable for infants and preparation method thereof |
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| CN114794485A (en) * | 2022-03-28 | 2022-07-29 | 上海启立生物科技有限公司 | A nutritional product for integrally regulating intestinal tract and immune health |
| CN116135047A (en) * | 2023-03-14 | 2023-05-19 | 河北新希望天香乳业有限公司 | Pure cereal probiotic composition and preparation method thereof |
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