CN107881133A - A kind of high-activity bifidobacterium powder vacuum drying production technology and application - Google Patents
A kind of high-activity bifidobacterium powder vacuum drying production technology and application Download PDFInfo
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Abstract
The invention discloses a kind of high-activity bifidobacterium powder vacuum drying production technology and application, belong to food technology field.The present invention is using Water insoluble dietary fiber as solid phase carrier, fermenting and producing Bifidobacterium biomembrane thalline；Collect bacterium mud after fermentation to mix with drying protectant, vacuum drying prepares Bifidobacteria powder.More than 50% is reached as high as using the Bifidobacterium vacuum drying survival rate of the inventive method, bacterium powder viable count reaches 15 × 1010Cfu/g, more than 10 times are improved relative to the Bifidobacterium vacuum drying survival rate of abiotic membrane stage.The present invention solves the problems, such as the key common technology for developing high-activity bifidobacterium powder, and the Bifidobacteria powder of preparation can apply in the product forms such as leavening and solid beverage.
Description
Technical field
The present invention relates to a kind of high-activity bifidobacterium powder vacuum drying production technology and application, belongs to food technology neck
Domain.
Background technology
Bifidobacterium is the autochthonous flora in human body and animal intestinal tract, is adjusted in intestinal microecology stabilization and host's physiological function
Play an important roll in terms of section.Therefore, from the 1980s, Bifidobacterium is widely used in fermented dairy product industry,
And it is applied to food and dietary supplements, there are wide market prospects in modern food industry.Commercially available common bifid
Bacillus live products have：Yoghourt, Bifidobacterium tablet, Bifidobacterium chocolate etc., these products are mostly by Bifidobacteria powder
It is obtained.Commercially available Bifidobacteria powder is made by the mode being freeze-dried more at this stage, although freeze-drying can obtain it is higher
The Bifidobacteria powder of survival rate, but be freeze-dried low etc. there is consumed energy in production equipment requirement height, drying process height, yield
Shortcoming, these problems greatly improve the preparation cost of Bifidobacteria powder.Vacuum drying has operation compared to freeze-drying
It is easy, equipment requirement is low, the advantages such as production cost is low, but vacuum drying also possesses the shortcomings that can not preparing high viability bacterium powder,
Therefore lifting Bifidobacterium vacuum drying survival rate is particularly important.
Patent CN201510872948.0, CN200310103234.0 and CN201110147416.2 improve Bifidobacterium and done
The method of dry survival rate focuses mostly in the research of Bifidobacterium dry-run protection agent prescription, passes through the sieve of the materials such as sugar, protein, salt
Choosing carrys out optimizing drying protection agent prescription so as to improve dry survival rate.The optimization of dry-run protection agent prescription, drying process condition
Although improvement, the application of microencapsulation technology can improve Bifidobacterium to a certain extent and dry survival rate, the above method carries
High level is limited, and complex for operation step, is unsuitable for large-scale industrialization application.
The content of the invention
The technical problem to be solved in the present invention is to overcome Bifidobacterium vacuum drying survival rate low, there is provided a kind of high activity,
Strong resistance Bifidobacteria powder production technology, vacuum drying survival rate and the intestines and stomach tolerance of Bifidobacterium can be significantly improved
Property, there is provided a kind of suitable Bifidobacteria powder prepares strategy.
First purpose of the present invention is to provide a kind of high activity, the production method of strong resistance Bifidobacteria powder, institute
The method of stating is to be added to by medium of Water insoluble dietary fiber in culture medium, and culture Bifidobacterium is to forming biomembrane, then receives
Collect Bifidobacterium biomembrane, be dried in vacuo.
In one embodiment of the invention, the Bifidobacterium biomembrane is containing Water insoluble dietary fiber
Cultivate and obtain in culture medium.
In one embodiment of the invention, the Water insoluble dietary fiber includes grain dust, beans powder, fruits and vegetables
The one or more of powder, mushroom powder and meals homogenous foodstuff pulvis.
In one embodiment of the invention, the Water insoluble dietary fiber includes analysis for soybean powder, buckwheat, celery
Powder, walnut shell powder, corn flour, black fungus powder, grape pip powder, hawthorn powder or hedgehog hydnum mushroom powder.
In one embodiment of the invention, the content of the Water insoluble dietary fiber is 10~30g/L.
In one embodiment of the invention, the Bifidobacterium includes false chainlet Bifidobacterium, bifidobacterium longum is grown
Subspecies, bifidobacterium longum baby subspecies, bifidobacterium adolescentis, bifidobacterium breve, bifidobacterium bifidum, animal bifidobacteria animal
Subspecies or bifidobacterium animalis subspecies.
In one embodiment of the invention, the culture medium contains：5~8g/L of dusty yeast, anhydrous sodium acetate 5~
8g/L, 10~16g/L of beef extract, 20~30g/L of DEXTROSE ANHYDROUS, 10~16g/L of peptone, bitter salt 0.5~
0.8g/L, 2~3g/L of dipotassium hydrogen phosphate, 0.25~0.4g/L of Manganous sulfate monohydrate, the Guang of diammonium hydrogen citrate 2~3g/L, L- half
Propylhomoserin 0.5~1.0g/L of hydrochloride, Tween-80 1g/L, 10~30g/L of Water insoluble dietary fiber.
In one embodiment of the invention, methods described comprises the following steps：1) Bifidobacterium is seeded to containing non-
In the culture medium of water-soluble dietary fiber；(2) in a nitrogen environment, in pH5.8~6.2,35~37 DEG C of 20~28h of culture；(3)
Thalline is collected by centrifugation；(4) bacterium mud is pressed：Drying protectant is 1:1~3 mass ratio adds drying protectant into bacterium mud, mixes；
(5) mixture of step (4) is dried in vacuo.
In one embodiment of the invention, the culture medium in the step (1) contains：Dusty yeast 5g/L, anhydrous second
Sour sodium 5g/L, beef extract 10g/L, DEXTROSE ANHYDROUS 20g/L, peptone 10g/L, bitter salt 0.5g/L, three hydration phosphorus
Sour hydrogen dipotassium 2.6g/L, Manganous sulfate monohydrate 0.25g/L, diammonium hydrogen citrate 2g/L, L-cysteine hydrochloride 0.5g/L, tells
Temperature -801g/L, analysis for soybean powder 10g/L.
In one embodiment of the invention, Bifidobacterium was activated for 3 generations by the step (2), according to volume ratio 3-5%
Inoculative proportion, be transferred in 10L fermentation tanks, be continually fed into nitrogen, adjust rotating speed, it is small that 37 degree constant pHs (pH=6) cultivate 24
When.
In one embodiment of the invention, the step (3) is carried out at 4 DEG C to Bifidobacterium biomembrane zymotic fluid
Bacterium mud is collected by centrifugation, centrifugal condition is：Rotating speed is 6000rpm/min, and centrifugation time is 10 minutes, obtains Bifidobacterium bacterium mud.
In one embodiment of the invention, dry-run protection agent prescription is in the step (4)：100g/L skimmed milks
Powder, 30g/L trehaloses, 30g/L sucrose, 0.5g/L cysteine hydrochlorides, pH 6.5.
In one embodiment of the invention, the step (5) is emulsion obtained by step (4) will to be laid in into flat board
On, it is dried in vacuo 18-24h under -0.1MPa under room temperature condition.
Second object of the present invention is to provide the bacterium powder prepared using methods described.
Third object of the present invention is to provide application of the bacterium powder in food, biological field, and the application includes system
Standby solid beverage, dairy products, fermentation fruits and vegetables, chocolate, candy, probiotics, probiotic tablet, probiotics capsule.
In one embodiment of the invention, the application includes preparing described in the food containing prebiotics containing prebiotics
Food contains at least one of FOS, galactooligosaccharide, xylo-oligosaccharide, breast milk oligosaccharide.
In one embodiment of the invention, described solid beverage product contain the freeze-dried bifidobacteria powder and
Prebiotics, the prebiotics include a kind of or more in FOS, galactooligosaccharide, xylo-oligosaccharide, breast milk oligosaccharide, inulin etc.
Kind.
In one embodiment of the invention, the composition of described solid beverage is：Bifidobacteria powder 0.1g
(final concentration of 109CFU/g), FOS 0.82g, galactooligosaccharide 0.68g, inulin 0.4g, 2g is often wrapped.
In one embodiment of the invention, the Bifidobacterium biomembrane bacterium powder is used for fermenting and producing dairy products, beans
Product or fruit and vegetable product.
Beneficial effect：The present invention by biological bacteria application of membrane in the fermenting and producing of Bifidobacterium, with water-insoluble meals
Fiber is eaten as solid interface, it is formed mycoderm in solid phase interface, reaches the effect for improving itself resistance, so as to significantly carry
High Bifidobacterium is dried in vacuo survival rate, and 3~5 times are improved compared to the bacterium powder survival rate not prepared using biological film technique,
Bacterium powder viable count reaches 1-5 × 1010Cfu/g, solves the crucial skill in exploitation high activity bifidobacteria vacuum drying bacterium powder
Art problem.High activity prepared by the present invention, strong resistance Bifidobacteria powder can be used in the products such as solid beverage, leavening,
The Water insoluble dietary fiber added in the present invention improves the function of bacterium powder as prebiotics.
Embodiment
Survival rate computational methods：Lyophilized bacterium powder is redissolved to lyophilized front volume with sterile distilled water, passes through flat board tilt-pour process
Viable bacteria concentration is determined, calculates lyophilized survival rate.Survival rate=100% × viable count after lyophilized/viable count before freezing.
Embodiment 1：The vacuum drying of false chainlet Bifidobacterium
(1) prepared by Bifidobacterium mycoderm fermentation medium：
It is prepared by false chainlet Bifidobacterium mycoderm fermentation medium：Dusty yeast 5g/L, anhydrous sodium acetate 5g/L, beef extract 10g/
L, DEXTROSE ANHYDROUS 20g/L, fish meal protein peptone 10g/L, bitter salt 0.5g/L, three hypophosphite monohydrate hydrogen dipotassium 2.6g/L,
Manganous sulfate monohydrate 0.25g/L, diammonium hydrogen citrate 2g/L, L-cysteine hydrochloride 0.5g/L, Tween-80 1g/L, soya bean
Powder 10g/L.
(2) prepared by the fermentation of Bifidobacterium mycoderm：Bifidobacterium was activated into for 3 generations, according to 3-5% inoculative proportion, switching
Into 10L fermentation tanks, nitrogen is continually fed into, adjusts rotating speed, 37 degree of constant pHs (pH=6) are cultivated 24 hours.
(3) collection of Bifidobacterium mycoderm：Bifidobacterium mycoderm zymotic fluid is carried out that bacterium mud is collected by centrifugation at 4 DEG C, from
Heart condition is：Rotating speed is 6000rpm/min, and centrifugation time is 10 minutes, obtains Bifidobacterium bacterium mud.
(4) drying protectant is added：By gained bacterium mud and drying protectant according to w/v (1:1) it is well mixed.
Above-mentioned dry-run protection agent prescription is：100g/L skimmed milk powders, 30g/L trehaloses, 30g/L sucrose, 0.5g/L cysteine hydrochloric acid
Salt, pH 6.5.
(5) it is dried in vacuo：By emulsion obtained by step (3), it is laid on flat board, vacuum drying condition is：Room temperature condition
Under be dried in vacuo 18h under -0.1MPa.Bifidobacteria powder can be obtained after vacuum drying.
After testing, the vacuum drying survival rate of mycoderm vacation chainlet Bifidobacterium is 41%.By bacterium powder in 25 degree of lower storages
12 weeks, survival rate 60%.
Reference examples 1
Bacterium powder preparation is carried out by the identical step of embodiment 1, is uniquely distinguished as not adding analysis for soybean powder in fermentation medium, its
Remaining condition is same as Example 1.Mycoderm is not formed after fermentation, survival rate is measured, is as a result shown, control group vacuum drying
Survival rate is 3%.Bacterium powder is deposited 12 weeks under 25 degree, survival rate 13%.
Embodiment 2
According to the operating procedure of embodiment 1, difference is, substitutes analysis for soybean powder with oat bran powder, carries out mycoderm fermentation, very
Sky is dried result and shown, the mycoderm vacuum drying survival rate formed by solid dielectric of oat bran powder is 47%, is significantly higher than
The thalline vacuum drying survival rate of non-mycoderm state.Bacterium powder is deposited 12 weeks under 25 degree, survival rate 49%.
Embodiment 3
According to the operating procedure of embodiment 1, difference is, changes drying protectant, and dry-run protection agent prescription is：100g/L
Skimmed milk powder, 30g/L stachyoses, 30g/L sucrose, 0.5g/L vitamin Cs, pH 6.5.Mycoderm vacation chainlet Bifidobacterium it is true
It is 45% that sky, which dries survival rate, is significantly higher than the thalline vacuum drying survival rate of non-mycoderm state.By bacterium powder in 25 degree of lower storages
12 weeks, survival rate 43%.
Embodiment 4：The vacuum drying of the long subspecies of bifidobacterium longum
(1) prepared by Bifidobacterium mycoderm fermentation medium：
Dusty yeast 6g/L, anhydrous sodium acetate 4g/L, beef extract 12g/L, DEXTROSE ANHYDROUS 25g/L, peptone 12g/L, seven
Magnesium sulfate heptahydrate 0.5g/L, three hypophosphite monohydrate hydrogen dipotassium 2.6g/L, Manganous sulfate monohydrate 0.25g/L, diammonium hydrogen citrate 2g/L,
L-cysteine hydrochloride 0.5g/L, Tween-80 1g/L, analysis for soybean powder 20g/L.
(2) prepared by the fermentation of Bifidobacterium mycoderm：Bifidobacterium was activated into for 3 generations, according to 3-5% inoculative proportion, switching
Into 10L fermentation tanks, nitrogen is continually fed into, adjusts rotating speed, 37 degree of constant pHs (pH=6) are cultivated 24 hours.
(3) collection of Bifidobacterium mycoderm：Bifidobacterium mycoderm zymotic fluid is carried out that bacterium mud is collected by centrifugation at 4 DEG C, from
Heart condition is：Rotating speed is 6000rpm/min, and centrifugation time is 10 minutes, obtains Bifidobacterium bacterium mud.
(4) drying protectant is added：By gained bacterium mud and drying protectant according to w/v (1:1) it is well mixed.
Above-mentioned dry-run protection agent prescription is：100g/L skimmed milk powders, 30g/L trehaloses, 30g/L sucrose, 0.5g/L cysteine hydrochloric acid
Salt, pH 6.5.
(5) it is dried in vacuo：By emulsion obtained by step (3), it is laid on flat board, vacuum drying condition is：Room temperature condition
Under be dried in vacuo 18-24h under -0.1MPa.Bifidobacteria powder can be obtained after vacuum drying.
After testing, the vacuum drying survival rate of the long subspecies of mycoderm bifidobacterium longum is 45%.Bacterium powder is deposited under 25 degree
Put 12 weeks, survival rate 57%.
Reference examples 2
Bacterium powder preparation is carried out by the identical step of embodiment 4, is uniquely distinguished as not adding analysis for soybean powder in fermentation medium, its
Remaining condition is same as Example 4.Fermentation process does not form mycoderm, and after testing, control group vacuum drying survival rate is 5%.By bacterium
Powder storage 12 weeks, survival rate 9% under 25 degree.
Embodiment 5
Operated according to the same steps of embodiment 4, difference is, is taken with buckwheat (15g/L) and celery powder (15g/L)
For analysis for soybean powder, mycoderm fermentation is carried out, vacuum drying result is shown, true as the mycoderm that solid dielectric is formed using buckwheat and celery powder
It is 50% that sky, which dries survival rate, is significantly higher than the thalline vacuum drying survival rate of non-mycoderm state.By bacterium powder in 25 degree of lower storages
12 weeks, survival rate 47%.
Embodiment 6
Operated according to the same steps of embodiment 4, difference is, vacuum drying protective agent is replaced with：120g/L takes off
Fat milk powder, 20g/L fucoses, 20g/L sucrose, 0.5g/L cysteine hydrochlorides, pH 6.5.Mycoderm bifidobacterium longum is grown
The vacuum drying survival rate of subspecies is 44%, is significantly higher than the thalline vacuum drying survival rate of non-mycoderm state.By bacterium powder in 25
The lower storage of degree 12 weeks, survival rate 57%.
Embodiment 7：The vacuum drying of bifidobacterium longum baby's subspecies
(1) prepared by Bifidobacterium mycoderm fermentation medium：Dusty yeast 6g/L, anhydrous sodium acetate 5g/L, beef extract 10g/L,
DEXTROSE ANHYDROUS 25g/L, peptone 10g/L, bitter salt 0.5g/L, three hypophosphite monohydrate hydrogen dipotassium 2.6g/L, one is hydrated
Manganese sulfate 0.25g/L, diammonium hydrogen citrate 2g/L, L-cysteine hydrochloride 0.5g/L, Tween-80 1g/L, analysis for soybean powder 20g/
L。
(2) prepared by the fermentation of Bifidobacterium mycoderm：Bifidobacterium was activated into for 3 generations, according to 3-5% inoculative proportion, switching
Into 10L fermentation tanks, nitrogen is continually fed into, adjusts rotating speed, 37 degree of constant pHs (pH=6) are cultivated 24 hours.
(3) collection of Bifidobacterium mycoderm：Bifidobacterium mycoderm zymotic fluid is carried out that bacterium mud is collected by centrifugation at 4 DEG C, from
Heart condition is：Rotating speed is 6000rpm/min, and centrifugation time is 10 minutes, obtains Bifidobacterium bacterium mud.
(4) drying protectant is added：By gained bacterium mud and drying protectant according to w/v (1:1) it is well mixed.
Above-mentioned dry-run protection agent prescription is：120g/L skimmed milk powders, 40g/L trehaloses, 0.5g/L cysteine hydrochlorides, pH 6.5.
(5) it is dried in vacuo：By emulsion obtained by step (3), it is laid on flat board, vacuum drying condition is：Room temperature condition
Under be dried in vacuo 18-24h under -0.1MPa.Bifidobacteria powder can be obtained after vacuum drying.
After testing, the vacuum drying survival rate of mycoderm bifidobacterium longum baby subspecies is 39%.By bacterium powder under 25 degree
Storage 12 weeks, survival rate 46%.
Reference examples 3
Bacterium powder preparation is carried out by the identical step of embodiment 7, is uniquely distinguished as not adding analysis for soybean powder in fermentation medium, its
Remaining condition is same as Example 7.As a result show, fermentation process does not form biomembrane, and bifidobacterium longum baby is sub- after vacuum drying
The survival rate of kind is 6%.
Embodiment 8
According to embodiment 7, analysis for soybean powder is substituted with walnut shell powder, carries out mycoderm fermentation, vacuum drying result is shown, with walnut
Shell powder is that the mycoderm vacuum drying survival rate that solid dielectric is formed is 39%, is significantly higher than the thalline vacuum drying of non-mycoderm state
Survival rate.Bacterium powder is deposited 12 weeks under 25 degree, survival rate 34%.
Embodiment 9
According to embodiment 7, difference is, frozen-dried protective agent prescription is：120g/L skimmed milk powders, 40g/L trehaloses,
0.5g/L cysteine hydrochlorides, pH 6.5.The vacuum drying survival rate of mycoderm bifidobacterium longum baby's subspecies is 35%,
It is significantly higher than the thalline vacuum drying survival rate of non-mycoderm state.Bacterium powder is deposited 12 weeks under 25 degree, survival rate 44%.
Embodiment 10：The vacuum drying of bifidobacterium bifidum
(1) prepared by Bifidobacterium mycoderm fermentation medium：According to weight ratio, the formula composition bag of mycoderm fermentation medium
Include：
Dusty yeast 5g/L, anhydrous sodium acetate 5g/L, beef extract 10g/L, DEXTROSE ANHYDROUS 25g/L, peptone 10g/L, seven
Magnesium sulfate heptahydrate 0.5g/L, three hypophosphite monohydrate hydrogen dipotassium 2.6g/L, Manganous sulfate monohydrate 0.25g/L, diammonium hydrogen citrate 2g/L,
L-cysteine hydrochloride 0.5g/L, Tween-80 1g/L, analysis for soybean powder 20g/L.
(2) prepared by the fermentation of Bifidobacterium mycoderm：Bifidobacterium was activated into for 3 generations, according to 3-5% inoculative proportion, switching
Into 10L fermentation tanks, nitrogen is continually fed into, adjusts rotating speed, 37 degree of constant pHs (pH=6) are cultivated 24 hours.
(3) collection of Bifidobacterium mycoderm：Bifidobacterium mycoderm zymotic fluid is carried out that bacterium mud is collected by centrifugation at 4 DEG C, from
Heart condition is：Rotating speed is 6000rpm/min, and centrifugation time is 10 minutes, obtains Bifidobacterium bacterium mud.
(4) drying protectant is added：By gained bacterium mud and drying protectant according to w/v (1:1) it is well mixed.
Above-mentioned dry-run protection agent prescription is：100g/L skimmed milk powders, 30g/L trehaloses, 30g/L sucrose, 0.5g/L cysteine hydrochloric acid
Salt, pH 6.5.
(5) it is dried in vacuo：By emulsion obtained by step (3), it is laid on flat board, vacuum drying condition is：Room temperature condition
Under be dried in vacuo 18h under -0.1MPa.Bifidobacteria powder can be obtained after vacuum drying.
After testing, the vacuum drying survival rate of mycoderm bifidobacterium bifidum is 33%.By bacterium powder in 25 degree of lower storages 12
Week, survival rate 40%.
Reference examples 4
Bacterium powder preparation is carried out by the identical step of embodiment 10, is uniquely distinguished as not adding analysis for soybean powder in fermentation medium,
Remaining condition is same as in Example 10.As a result show, fermentation process does not form mycoderm, and the bifidobacterium bifidum for not forming mycoderm is true
It is 3% that sky, which dries survival rate,.Bacterium powder is deposited 12 weeks under 25 degree, survival rate 11%.
Embodiment 11
According to embodiment 10, analysis for soybean powder is substituted with corn flour, carries out biological film fermentation, vacuum drying result is shown, with jade
Ground rice is that the biomembrane vacuum drying survival rate that solid dielectric is formed is 30%, is significantly higher than the thalline vacuum of abiotic membrane stage
Dry survival rate.Bacterium powder is deposited 12 weeks under 25 degree, survival rate 41%.
Embodiment 12
According to embodiment 10, vacuum drying protective agent is changed, new vacuum drying protection agent prescription is：100g/L whey eggs
In vain, 20g/L trehaloses, 5g/L sodium glutamates, 0.5g/L cysteine hydrochlorides, pH 6.5.Mycoderm bifidobacterium bifidum
It is 38% to be dried in vacuo survival rate, is significantly higher than the thalline vacuum drying survival rate of non-mycoderm state.Bacterium powder is deposited under 25 degree
Put 12 weeks, survival rate 35%.
Embodiment 13：The vacuum drying of bifidobacterium adolescentis
(1) prepared by Bifidobacterium mycoderm fermentation medium：Dusty yeast 6g/L, anhydrous sodium acetate 5g/L, beef extract 12g/L,
DEXTROSE ANHYDROUS 25g/L, peptone 12g/L, bitter salt 0.5g/L, three hypophosphite monohydrate hydrogen dipotassium 2.0g/L, one is hydrated
Manganese sulfate 0.25g/L, diammonium hydrogen citrate 3g/L, L-cysteine hydrochloride 0.5g/L, Tween-80 1g/L, analysis for soybean powder 20g/
L。
(2) prepared by the fermentation of Bifidobacterium mycoderm：Bifidobacterium was activated into for 3 generations, according to 3-5% inoculative proportion, switching
Into 10L fermentation tanks, nitrogen is continually fed into, adjusts rotating speed, 37 degree of constant pHs (pH=6) are cultivated 24 hours.
(3) collection of Bifidobacterium mycoderm：Bifidobacterium mycoderm zymotic fluid is carried out that bacterium mud is collected by centrifugation at 4 DEG C, from
Heart condition is：Rotating speed is 6000rpm/min, and centrifugation time is 10 minutes, obtains Bifidobacterium bacterium mud.
(4) drying protectant is added：By gained bacterium mud and drying protectant according to w/v (1:1) it is well mixed.
Above-mentioned dry-run protection agent prescription is：100g/L skimmed milk powders, 30g/L trehaloses, 30g/L sucrose, 0.5g/L cysteine hydrochloric acid
Salt, pH 6.5.
(5) it is dried in vacuo：By emulsion obtained by step (3), it is laid on flat board, vacuum drying condition is：Room temperature condition
Under be dried in vacuo 18h under -0.1MPa.Bifidobacteria powder can be obtained after vacuum drying.
After testing, the vacuum drying survival rate of mycoderm bifidobacterium adolescentis is 32%.By bacterium powder in 25 degree of lower storages 12
Week, survival rate 36%.
Reference examples 5
Bacterium powder preparation is carried out by the identical step of embodiment 13, is uniquely distinguished as not adding analysis for soybean powder in fermentation medium,
Remaining condition is identical with embodiment 13.Mycoderm is not formed in fermentation process, the survival rate after vacuum drying is measured, as a result
It has been shown that, survival rate 2%.
Embodiment 14
Implemented according to the step of embodiment 13, difference is, substitutes analysis for soybean powder with hedgehog hydnum mushroom powder, carries out mycoderm hair
Ferment, vacuum drying result show that the mycoderm vacuum drying survival rate formed by solid dielectric of hedgehog hydnum mushroom powder is 38%, significantly high
Survival rate is dried in vacuo in the thalline of non-mycoderm state.Bacterium powder is deposited 12 weeks under 25 degree, survival rate 41%.
Embodiment 15
The step of according to embodiment 13, difference is, vacuum drying protection agent prescription is：100g/L skimmed milk powders, 20g/L
Trehalose, 10g/L inulin, 5g/L sodium glutamates, 0.5g/L cysteine hydrochlorides, pH 6.5.Mycoderm bifidobacterium adolescentis
The dry survival rate of vacuum drying be 40%, be significantly higher than non-mycoderm state thalline vacuum drying survival rate.By bacterium powder in 25 degree
Lower storage 12 weeks, survival rate 36%.
Embodiment 16：The vacuum drying of bifidobacterium breve
(1) prepared by Bifidobacterium mycoderm fermentation medium：Dusty yeast 6g/L, anhydrous sodium acetate 5g/L, beef extract 12g/L,
DEXTROSE ANHYDROUS 20g/L, peptone 10g/L, bitter salt 0.5g/L, three hypophosphite monohydrate hydrogen dipotassium 2.6g/L, one is hydrated
Manganese sulfate 0.25g/L, diammonium hydrogen citrate 2g/L, L-cysteine hydrochloride 0.5g/L, Tween-80 1g/L, analysis for soybean powder 20g/
L。
(2) prepared by the fermentation of Bifidobacterium mycoderm：Bifidobacterium was activated into for 3 generations, according to 3-5% inoculative proportion, switching
Into 10L fermentation tanks, nitrogen is continually fed into, adjusts rotating speed, 37 degree of constant pHs (pH=6) are cultivated 24 hours.
(3) collection of Bifidobacterium mycoderm：Bifidobacterium mycoderm zymotic fluid is carried out that bacterium mud is collected by centrifugation at 4 DEG C, from
Heart condition is：Rotating speed is 6000rpm/min, and centrifugation time is 10 minutes, obtains Bifidobacterium bacterium mud.
(4) drying protectant is added：By gained bacterium mud and drying protectant according to w/v (1:1) it is well mixed.
Above-mentioned dry-run protection agent prescription is：100g/L skimmed milk powders, 30g/L trehaloses, 30g/L sucrose, 0.5g/L cysteine hydrochloric acid
Salt, pH 6.5.
(5) it is dried in vacuo：By emulsion obtained by step (3), it is laid on flat board, vacuum drying condition is：Room temperature condition
Under be dried in vacuo 18-24h under -0.1MPa.Bifidobacteria powder can be obtained after vacuum drying.
After testing, the vacuum drying survival rate of mycoderm bifidobacterium breve is 35%.Bacterium powder is deposited 12 weeks under 25 degree,
Survival rate is 37%.
Reference examples 6
Bacterium powder preparation is carried out by the identical step of embodiment 16, is uniquely distinguished as not adding analysis for soybean powder in fermentation medium,
Remaining condition is identical with embodiment 16.Mycoderm is not formed in fermentation process, the survival rate after vacuum drying is 2%.By bacterium powder in
Storage 12 weeks, survival rate 9% under 25 degree.
Embodiment 17
According to embodiment 16, analysis for soybean powder is substituted with auricularia auriculajudae powder, carries out mycoderm fermentation, vacuum drying result is shown, with agaric
Powder is that the mycoderm vacuum drying survival rate that solid dielectric is formed is 38%, and the thalline vacuum drying for being significantly higher than non-mycoderm state is deposited
Motility rate.Bacterium powder is deposited 12 weeks under 25 degree, survival rate 41%.
Embodiment 18
According to embodiment 16, difference is, is dried in vacuo protectant formula and is：100g/L skimmed milk powders, 20g/L marine algas
Sugar, 10g/L inulin, 5g/L sodium glutamates, 0.5g/L cysteine hydrochlorides, pH 6.5.The vacuum of mycoderm bifidobacterium breve
It is 30% to dry survival rate, is significantly higher than the thalline vacuum drying survival rate of non-mycoderm state.By bacterium powder in 25 degree of lower storages 12
Week, survival rate 30%.
Embodiment 19：The vacuum drying of animal bifidobacteria animal subspecies
(1) prepared by Bifidobacterium mycoderm fermentation medium：Dusty yeast 6g/L, anhydrous sodium acetate 5g/L, beef extract 12g/L,
DEXTROSE ANHYDROUS 20g/L, peptone 10g/L, bitter salt 0.5g/L, three hypophosphite monohydrate hydrogen dipotassium 2.6g/L, one is hydrated
Manganese sulfate 0.25g/L, diammonium hydrogen citrate 2g/L, L-cysteine hydrochloride 0.5g/L, Tween-80 1g/L, analysis for soybean powder 20g/
L。
(2) prepared by the fermentation of Bifidobacterium mycoderm：Bifidobacterium was activated into for 3 generations, according to 3-5% inoculative proportion, switching
Into 10L fermentation tanks, nitrogen is continually fed into, adjusts rotating speed, 37 degree of constant pHs (pH=6) are cultivated 24 hours.
(3) collection of Bifidobacterium mycoderm：Bifidobacterium mycoderm zymotic fluid is carried out that bacterium mud is collected by centrifugation at 4 DEG C, from
Heart condition is：Rotating speed is 6000rpm/min, and centrifugation time is 10 minutes, obtains Bifidobacterium bacterium mud.
(4) drying protectant is added：By gained bacterium mud and drying protectant according to w/v (1:1) it is well mixed.
Above-mentioned dry-run protection agent prescription is：100g/L skimmed milk powders, 30g/L trehaloses, 30g/L sucrose, 0.5g/L cysteine hydrochloric acid
Salt, pH 6.5.
(5) it is dried in vacuo：By emulsion obtained by step (3), it is laid on flat board, vacuum drying condition is：Room temperature condition
Under be dried in vacuo 18-24h under -0.1MPa.Bifidobacteria powder can be obtained after vacuum drying.
After testing, the vacuum drying survival rate of mycoderm animal bifidobacteria is 38%.By bacterium powder in 25 degree of lower storages 12
Week, survival rate 41%.
Reference examples 7
Bacterium powder preparation is carried out by the identical step of embodiment 19, is uniquely distinguished as not adding analysis for soybean powder in fermentation medium,
Remaining condition is identical with embodiment 19.Mycoderm is not formed in fermentation process, vacuum drying survival rate is 3%.By bacterium powder in 25 degree
Lower storage 12 weeks, survival rate 16%.
Embodiment 20
According to embodiment 19, difference is, substitutes analysis for soybean powder with grape pip powder, carries out mycoderm fermentation, is dried in vacuo result
It has been shown that, the mycoderm vacuum drying survival rate formed by solid dielectric of grape pip powder is 42%, is significantly higher than non-mycoderm state
Thalline is dried in vacuo survival rate.Bacterium powder is deposited 12 weeks under 25 degree, survival rate 46%.
Embodiment 21
According to embodiment 19, difference is, vacuum drying protection agent prescription is：120g/L skimmed milk powders, 40g/L marine algas
Sugar, 0.5g/L cysteine hydrochlorides, pH 6.5.The vacuum drying survival rate of mycoderm animal bifidobacteria is 33%, significantly
Thalline higher than non-mycoderm state is dried in vacuo survival rate.
Embodiment 22：The vacuum drying of bifidobacterium animalis subspecies
(1) prepared by Bifidobacterium mycoderm fermentation medium：Dusty yeast 5g/L, anhydrous sodium acetate 5g/L, beef extract 12g/L,
DEXTROSE ANHYDROUS 20g/L, peptone 10g/L, bitter salt 0.5g/L, three hypophosphite monohydrate hydrogen dipotassium 2.6g/L, one is hydrated
Manganese sulfate 0.25g/L, diammonium hydrogen citrate 2g/L, L-cysteine hydrochloride 0.5g/L, Tween-80 1g/L, analysis for soybean powder 20g/
L.(2) prepared by the fermentation of Bifidobacterium mycoderm：Bifidobacterium was activated into for 3 generations, according to 3-5% inoculative proportion, is transferred to 10L hairs
In fermentation tank, nitrogen is continually fed into, adjusts rotating speed, 37 degree of constant pHs (pH=6) are cultivated 24 hours.
(3) collection of Bifidobacterium mycoderm：Bifidobacterium mycoderm zymotic fluid is carried out that bacterium mud is collected by centrifugation at 4 DEG C, from
Heart condition is：Rotating speed is 6000rpm/min, and centrifugation time is 10 minutes, obtains Bifidobacterium bacterium mud.
(4) drying protectant is added：By gained bacterium mud and drying protectant according to w/v (1:1) it is well mixed.
Above-mentioned dry-run protection agent prescription is：100g/L skimmed milk powders, 30g/L trehaloses, 30g/L sucrose, 0.5g/L cysteine hydrochloric acid
Salt, pH 6.5.
(5) it is dried in vacuo：By emulsion obtained by step (3), it is laid on flat board, vacuum drying condition is：Room temperature condition
Under be dried in vacuo 18-24h under -0.1MPa.Bifidobacteria powder can be obtained after vacuum drying.
After testing, the vacuum drying survival rate of mycoderm bifidobacterium animalis subspecies is 52%.By bacterium powder under 25 degree
Storage 12 weeks, survival rate 45%.
Reference examples 8
Bacterium powder preparation is carried out by the identical step of embodiment 22, is uniquely distinguished as not adding analysis for soybean powder in fermentation medium,
Remaining condition is identical with embodiment 22.Mycoderm is not formed in fermentation process, vacuum drying survival rate is 6%.By bacterium powder in 25 degree
Lower storage 12 weeks, survival rate 13%.
Embodiment 23
According to embodiment 22, analysis for soybean powder is substituted with hawthorn powder, carries out mycoderm fermentation, vacuum drying result is shown, with hawthorn
Powder is that the mycoderm vacuum drying survival rate that solid dielectric is formed is 50%, and the thalline vacuum drying for being significantly higher than non-mycoderm state is deposited
Motility rate.Bacterium powder is deposited 12 weeks under 25 degree, survival rate 46%.
Embodiment 24
According to embodiment 22, difference is, vacuum drying protection agent prescription is：80g/L skimmed milk powders, 20g/L trehaloses,
20g/L sucrose, 10g/L inulin, 5g/L sodium glutamates, 0.5g/L cysteine hydrochlorides, pH 6.5.Mycoderm animal bifid
The dry survival rate of vacuum drying of bacillus is 33%, is significantly higher than the thalline vacuum drying survival rate of non-mycoderm state.
Although the present invention is disclosed as above with preferred embodiment, it is not limited to the present invention, any to be familiar with this skill
The people of art, without departing from the spirit and scope of the present invention, it can all do various change and modification, therefore the protection model of the present invention
Enclose being defined of being defined by claims.
Claims (10)
1. a kind of production method of Bifidobacteria powder, it is characterised in that trained with the culture medium containing Water insoluble dietary fiber
Foster Bifidobacterium regathers Bifidobacterium biomembrane, is dried in vacuo to biomembrane is formed.
2. according to the method for claim 1, it is characterised in that the Water insoluble dietary fiber include grain dust, bean powder,
The one or more of fruit vegetable powder, mushroom powder and meals homogenous foodstuff pulvis.
3. according to the method for claim 2, it is characterised in that the Water insoluble dietary fiber includes analysis for soybean powder, buckwheat
Powder, celery powder, walnut shell powder, corn flour, black fungus powder, grape pip powder, hawthorn powder or hedgehog hydnum mushroom powder.
4. according to any described methods of claim 1-3, it is characterised in that the content of the Water insoluble dietary fiber is 10
~30g/L.
5. according to the method for claim 1, it is characterised in that it is double that the Bifidobacterium includes false chainlet Bifidobacterium, length
The long subspecies of discrimination bacillus, bifidobacterium longum baby subspecies, bifidobacterium adolescentis, bifidobacterium breve, bifidobacterium bifidum, animal bifid
Bacillus animal subspecies or bifidobacterium animalis subspecies.
6. according to the method for claim 4, it is characterised in that comprise the following steps：1) Bifidobacterium is seeded to containing non-
In the culture medium of water-soluble dietary fiber；(2) in 5.8~6.2,35~37 DEG C of 20~28h of Anaerobic culturel of pH；(3) it is collected by centrifugation
Bacterium mud；(4) bacterium mud is pressed：Drying protectant is 1:1~3 mass ratio adds drying protectant into bacterium mud, mixes；(5) will step
Suddenly the mixture vacuum drying of (4).
7. according to the method for claim 6, it is characterised in that the formula of drying protectant is in the step (4)：0~
120g/L skimmed milk powders, 0~40g/L trehaloses, 0~40g/L sucrose, 0~40g/L stachyoses, 0~30g/L inulin, 0~5g/
L-Glu sodium, 0~10g/L inulin, 0~0.5g/L cysteine hydrochlorides and 0~0.5g/L vitamin Cs.
8. bacterium powder prepared by any methods describeds of claim 1-2,5-7.
9. bacterium powder described in claim 8 prepares solid beverage, dairy products, fermentation fruits and vegetables, chocolate, sugar in food, biological field
Application in fruit, probiotics, probiotic tablet, probiotics capsule.
10. food, medicine, health products containing bacterium powder described in claim 8.
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