CN113813451B

CN113813451B - Preparation method and equipment of autologous concentrated growth factor membrane

Info

Publication number: CN113813451B
Application number: CN202111220874.4A
Authority: CN
Inventors: 马斐斐; 马玉; 孙凤
Original assignee: Peking University School of Stomatology
Current assignee: Peking University School of Stomatology
Priority date: 2021-10-20
Filing date: 2021-10-20
Publication date: 2022-08-12
Anticipated expiration: 2041-10-20
Also published as: CN113813451A

Abstract

The application discloses a preparation method of an autologous concentrated growth factor membrane, which comprises the following steps: putting the blood to be centrifuged into a centrifuge for blood centrifugation to obtain a solid autologous platelet aggregate; extracting an autologous concentrated growth factor gel from the solid autologous platelet aggregates; placing the autologous concentrated growth factor gel in a film pressing device for film pressing treatment to form a first autologous concentrated growth factor film; pressing a plurality of the first autologous concentrated growth factor membranes into second autologous concentrated growth factor membranes. In the embodiment of the application, the first autologous concentrated growth factor membrane is formed by carrying out film pressing treatment on the autologous concentrated growth factor gel, and then the second autologous concentrated growth factor membrane is formed by pressing a plurality of first autologous concentrated growth factor membranes, so that the second autologous concentrated growth factor membrane becomes a complete large membrane and is not easy to separate, the area of the membrane is increased, and the membrane is more suitable for covering the maxillary sinus floor mucosa.

Description

Preparation method and equipment of autologous concentrated growth factor membrane

Technical Field

The application relates to a medical material preparation technology, in particular to a preparation method and equipment of an autologous concentrated growth factor membrane.

Background

In the oral implantation operation, alveolar bone defect is a common problem, and the classical guided bone regeneration operation is an effective means for solving the problem. During this procedure, a heterogeneous bone material is implanted into the bone defect area and covered with a biofilm.

Practice shows that in the maxillary sinus lifting operation, the sinus floor mucosa can be better protected by the inferior tectorial membrane of the sinus floor mucosa, and the mucosa perforation caused by friction of bone particles is not easy to occur. Currently, collagen membranes are frequently used clinically with additional charges. The autologous concentrated Growth factor membrane (CGF for short) can be used for replacing a collagen membrane under the sinus floor mucosa, has better adhesiveness, contains Growth factors and can promote tissue regeneration, but the autologous concentrated Growth factor membrane has the problem of limited size and can not completely cover the sinus floor mucosa of the maxillary sinus.

Disclosure of Invention

The embodiment of the application provides a preparation method of an autologous concentrated growth factor membrane, and solves the problems that the autologous concentrated growth factor membrane is limited in size and cannot completely cover the mucosa at the bottom of the maxillary sinus.

The embodiment of the application provides a preparation method of an autologous concentrated growth factor membrane, which comprises the following steps: putting the blood to be centrifuged into a centrifuge for blood centrifugation to obtain a solid autologous platelet aggregate; extracting autologous concentrated growth factor gel from the solid autologous platelet aggregates; placing the autologous concentrated growth factor gel in a film pressing device for film pressing treatment to form a first autologous concentrated growth factor film; pressing a plurality of the first autologous concentrated growth factor membranes into second autologous concentrated growth factor membranes.

Optionally, the solid autologous platelet aggregate comprises: serum, fibrin gel and red blood cell layer.

Optionally, the extracting autologous concentrated growth factor gel from the solid autologous platelet aggregate comprises:

filtering the solid autologous platelet aggregates in a separator to obtain filtered solid autologous platelet aggregates;

extracting autologous concentrated growth factor gel from said filtered solid autologous platelet aggregates.

Optionally, the step of placing the autologous concentrated growth factor gel in a membrane pressing device for membrane pressing treatment to form a first autologous concentrated growth factor membrane includes: and placing the autologous concentrated growth factor gel taken out from the physiological saline into a film pressing device for film pressing treatment to form a first autologous concentrated growth factor film.

Optionally, when the first autologous concentrated growth factor membrane is used for the maxillary sinus lifting operation, the first autologous concentrated growth factor membrane is pressed into a flat shape.

Optionally, the first autologous concentrated growth factor membrane has a thickness of 800 microns.

Optionally, when the second autologous concentrated growth factor membrane is used for the maxillary sinus external lifting operation, the plurality of first autologous concentrated growth factor membranes are pressed into the second autologous concentrated growth factor membrane.

Optionally, the second autologous concentrated growth factor membrane is used to cover the inframucosal membrane of the maxillary sinus, and the maxillary sinus is a fenestrated maxillary sinus;

the pressing of the plurality of first autologous concentrated growth factor membranes into a second autologous concentrated growth factor membrane comprises: and pressing a plurality of first self-concentration growth factor films corresponding to the perforating positions, so that the thickness of the film at the perforating positions is thicker than that of the surrounding parts, and the second self-concentration growth factor films are used.

Optionally, the method further includes: venous blood of the patient is drawn as the blood to be centrifuged.

Optionally, the number of the plurality of the first autologous concentrated growth factor membranes is 4.

Optionally, the second autologous concentrated growth factor membrane has a thickness of 200 microns.

The embodiment of the application also provides preparation equipment of the autologous concentrated growth factor membrane, which comprises a first shell, a centrifugal device arranged in the first shell and a second shell arranged in the first shell; a blood collection tube inlet for placing a blood collection tube containing blood to be centrifuged into the centrifuge is formed in the first housing; a normal saline injection port for injecting normal saline into the second shell is arranged on the first shell, and a normal saline conveying pipe for conveying normal saline is arranged between the normal saline injection port and the normal saline inlet of the second shell; an autologous concentrated growth factor gel conveying pipe for conveying autologous concentrated growth factor gel is arranged between the centrifugal device and the second shell; a physiological saline storage device, a first membrane pressing device and a second membrane pressing device are arranged in the second shell; the physiological saline storage is used for storing a first self-concentration growth factor film, the first film pressing device is used for performing film pressing treatment on the self-concentration growth factor gel to form a first self-concentration growth factor film, and the second film pressing device is used for performing film pressing treatment on a plurality of first self-concentration growth factor films to form a second self-concentration growth factor film;

Wherein, a blood collection tube filled with blood to be centrifugally treated is placed into the centrifugal device through the blood collection tube placement opening, physiological saline is injected into the physiological saline storage of the second shell through the physiological saline conveying tube, the centrifugal device carries out centrifugal treatment on the blood to obtain solid autologous platelet aggregate, autologous concentrated growth factor gel is extracted from the solid autologous platelet aggregate, the extracted autologous concentrated growth factor gel is conveyed into the physiological saline storage of the second shell through the autologous concentrated growth factor gel conveying tube, the treated autologous concentrated growth factor gel is taken out from the physiological saline storage, the autologous concentrated growth factor gel is placed into a first film pressing device for film pressing treatment to form a first autologous concentrated growth factor film, and conveying a plurality of the first self-concentration growth factor films into a second film pressing device for film pressing treatment to form second self-concentration growth factor films.

Optionally, an autologous concentrated growth factor gel detection device and an autologous concentrated growth factor gel extraction device are arranged in the centrifugal device;

the autologous concentrated growth factor gel detection device is used for detecting autologous concentrated growth factor gel from the solid autologous platelet aggregate after the centrifugation device obtains the solid autologous platelet aggregate, and sending an autologous concentrated growth factor gel extraction instruction to the autologous concentrated growth factor gel extraction device after the autologous concentrated growth factor gel is detected;

the autologous concentrated growth factor gel extraction device is used for extracting autologous concentrated growth factor gel from the solid autologous platelet aggregate according to the extraction instruction.

Optionally, the autologous concentrated growth factor gel detection device includes a first image collector and a first image analyzer;

the first image collector is used for collecting an image of the solid autologous platelet aggregate and sending the image of the solid autologous platelet aggregate to the image analyzer;

The first image analyzer is used for positioning the position of the fibrin gel in the image of the solid autologous platelet aggregate to obtain positioning information and sending the positioning information to the autologous concentrated growth factor gel extraction device;

the autologous concentrated growth factor gel extraction device extracts the fibrin gel part from the solid autologous platelet aggregate according to the positioning information.

Optionally, a transmission mechanism for transmitting the second housing to the outside of the first housing is further disposed in the first housing;

after forming a second autologous concentrated growth factor membrane, the transmission mechanism transfers the second housing out of the first housing.

Optionally, when the first autologous concentrated growth factor membrane is used for the maxillary sinus lifting operation, the first membrane pressing device presses the first autologous concentrated growth factor membrane into a flat shape.

Optionally, when the second autologous concentrated growth factor membrane is used for the maxillary sinus external lifting operation, the second membrane pressing device presses the plurality of first autologous concentrated growth factor membranes into the second autologous concentrated growth factor membrane.

Optionally, the second autologous concentrated growth factor membrane is used to cover the inframucosal membrane of the maxillary sinus, if the maxillary sinus is a fenestrated maxillary sinus;

And the second film pressing device presses the plurality of first autologous concentrated growth factor films arranged at the perforating positions so as to enable the film thickness at the perforating positions to be thicker than the surrounding parts to be used as second autologous concentrated growth factor films.

Optionally, a second autologous concentrated growth factor membrane detection device is arranged in the second shell;

the second autologous concentrated growth factor membrane detection device is used for sending a first transmission instruction for transmitting the second shell to the outside of the first shell to the transmission mechanism after detecting the second autologous concentrated growth factor membrane;

the transmission mechanism is specifically used for transmitting the second shell to the outside of the first shell according to the first transmission instruction.

Optionally, the second autologous concentrated growth factor membrane detection device includes a second image collector and a second image analyzer;

the second image collector is used for collecting images in the second shell and sending the images in the second shell to the second image analyzer;

the second image analyzer is used for analyzing the image in the second shell and sending the transmission instruction to the transmission mechanism if the image of the second autologous condensed growth factor membrane is detected in the image in the second shell.

Optionally, the transmission mechanism is further configured to transmit the second housing into the first housing after obtaining a second transmission instruction for transmitting the second housing into the first housing.

Optionally, a movable trigger button assembly for triggering the second housing to move is arranged on the first housing;

the mobile trigger button assembly is used for responding to a trigger operation aiming at the mobile trigger button assembly, judging the position of the second shell, and sending a second transmission instruction for transmitting the second shell into the first shell to the transmission mechanism if the second shell is outside the first shell.

the mobile trigger button assembly is used for responding to a trigger operation aiming at the mobile trigger button assembly, judging the position of the second shell, and sending a first transmission instruction for transmitting the second shell to the outside of the first shell to the transmission mechanism if the second shell is in the first shell.

Compared with the prior art, the embodiment of the application has the following advantages:

In the embodiment of the application, the first autologous concentrated growth factor membrane is formed by carrying out film pressing treatment on the autologous concentrated growth factor gel, and then the second autologous concentrated growth factor membrane is formed by pressing a plurality of first autologous concentrated growth factor membranes, so that the second autologous concentrated growth factor membrane becomes a complete large membrane and is not easy to separate, the area of the membrane is increased, and the membrane is more suitable for covering the maxillary sinus floor mucosa.

In the preferred embodiment of this application, can select different press mold modes according to the maxillary sinus size of windowing, when first autologous concentrated growth factor membrane is used for the interior promotion art of maxillary sinus, will first autologous concentrated growth factor membrane is pressed into the platykurtic. And when the second autologous concentrated growth factor membrane is used for the maxillary sinus external lifting operation, the plurality of first autologous concentrated growth factor membranes are pressed into the second autologous concentrated growth factor membrane. And if the maxillary sinus is the maxillary sinus with a perforation, pressing a plurality of first autologous concentrated growth factor membranes corresponding to the perforation positions to enable the membrane thickness at the perforation positions to be thicker than the peripheral part to be used as a second autologous concentrated growth factor membrane.

Drawings

FIG. 1 is a flow chart of a method for preparing an autologous concentrated growth factor membrane according to the embodiment of the present application;

FIG. 2 is a schematic diagram of blood to be centrifuged as provided in an embodiment of the present application;

fig. 3 is a schematic diagram of a solid autologous platelet aggregate provided in an embodiment of the present application;

FIG. 4 is a first schematic diagram of an apparatus for preparing an autologous concentrated growth factor membrane according to the embodiment of the present application;

fig. 5 is a second schematic diagram of an apparatus for preparing an autologous concentrated growth factor membrane provided in the embodiments of the present application.

Detailed Description

In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present application. This application is capable of implementation in many different ways than those herein set forth and of similar import by those skilled in the art without departing from the spirit of this application and is therefore not limited to the specific implementations disclosed below.

The process is described and illustrated in detail below by means of specific examples.

Fig. 1 is a flowchart of a method for preparing an autologous concentrated growth factor membrane according to an embodiment of the present disclosure, and please refer to fig. 1, the method includes the following steps:

s101: putting the blood to be centrifuged into a centrifuge for blood centrifugation to obtain a solid autologous platelet aggregate;

in the embodiment of this application, treat that centrifugal treatment's blood is patient's venous blood, after extracting patient's venous blood, put into the centrifuging tube with centrifuge matching with it, put into centrifuge again with the centrifuging tube and carry out blood centrifugation, obtain venous blood after the centrifugal treatment, solid-state autologous platelet aggregate promptly.

Fig. 2 is a schematic diagram of blood to be centrifuged according to an embodiment of the present application, and fig. 3 is a schematic diagram of solid autologous platelet aggregates according to an embodiment of the present application. Referring to fig. 2, venous blood of a patient is extracted as blood 202 to be centrifuged, and the blood 202 to be centrifuged is introduced into a centrifuge tube 201 fitted to a centrifuge. Referring to fig. 3, the solid autologous platelet aggregate 301 can be obtained by placing the centrifuge tube 201 into a centrifuge and centrifuging the blood.

The steps of the method for obtaining the solid autologous platelet aggregate 301 can be implemented by an apparatus, referring to fig. 4, comprising a first housing 400, a centrifugation device 401, and a blood collection tube inlet 403. Wherein, the centrifuge 401 is disposed in the first housing 400, the blood collection tube inlet 403 is disposed on the first housing 400, and is used for placing a blood collection tube 419 into the centrifuge 401, wherein a shielding object, such as a cover, which is matched and sealed with the blood collection tube inlet 403, can be disposed on the blood collection tube inlet 403 to prevent dust from entering the device. The blood collection tube 419 containing the blood to be centrifuged is inserted into the centrifuge apparatus 401 through the blood collection tube insertion port 403, and the blood to be centrifuged is centrifuged by the centrifuge apparatus 401, whereby a solid autologous platelet aggregate can be obtained.

The blood collection tube 419 is a disposable blood collection tube, and the blood collection tube can be inserted into the centrifuge 401 through the blood collection tube insertion port 403, or the used blood collection tube can be removed from the housing 400 to replace the blood collection tube.

S102: extracting an autologous concentrated growth factor gel from the solid autologous platelet aggregates;

The autologous concentrated Growth factors, CGF (concentrated Growth factors) for short, are a new generation of Growth factors, contain concentrated Growth factors and fibrin, and have the unique property of improving and enhancing tissue regeneration. The autologous concentrated growth factor is prepared by taking venous blood of a patient as a raw material through a special separation method.

In an embodiment of the present application, the extracting autologous concentrated growth factor gel from the solid autologous platelet aggregate comprises: filtering the solid autologous platelet aggregates in a separator to obtain filtered solid autologous platelet aggregates; extracting autologous concentrated growth factor gel from said filtered solid autologous platelet aggregates. Wherein the solid autologous platelet aggregates are filtered in a separator, mainly to filter out the liquid fraction.

The solid autologous platelet aggregate comprises: serum, fibrin gel, and red blood cell layer.

In specific operation, please continue to refer to fig. 2 and fig. 3, when preparing the autologous concentrated growth factor gel, firstly, the venous blood of the patient is extracted, and the venous blood of the patient is placed in a centrifuge tube 201 matched with a centrifuge, in order to prevent blood coagulation, the centrifuge tube 201 containing the blood 202 to be centrifuged should be placed in the centrifuge as soon as possible for centrifugation, for example, blood centrifugation is performed within 2 minutes, and then the centrifuge is started, and the specific centrifugation process is as follows: the centrifuge was accelerated to 2700 rpm in 30 seconds, then the centrifuge was centrifuged at 2700 rpm for 2 minutes, 2400 rpm for 4 minutes, then at 2700 rpm for 2 minutes, and at 3000 rpm for 3 minutes, and finally the centrifuge was decelerated in 36 seconds and stopped. Standing the centrifuged centrifuge tube 201 filled with blood to obtain the solid autologous platelet aggregate 301, wherein the solid autologous platelet aggregate 301 is divided into three layers from top to bottom: (1) the upper layer is serum (platelet poor plasma lacking fibrinogen and coagulation factors) 302; (2) the lower layer is the red blood cell layer 304; (3) the middle layer is fibrin gel which is provided with a large amount of platelets and growth factors, namely, the autologous concentrated growth factor gel 303, wherein the autologous concentrated growth factor gel 303 is divided into 3 layers: the white portion 303-1 of the upper layer, the red portion 303-3 of the lower layer, and the middle red-white intersecting portion 303-2. In the present example, the fibrin gel layer of the solid autologous platelet aggregate is rich in growth factors.

The steps of the method for extracting the autologous concentrated growth factor gel 303 can be implemented by the following equipment, please refer to fig. 4, wherein a separator 406 is disposed in the centrifugal device 401, and the method for extracting the autologous concentrated growth factor gel from the solid autologous platelet aggregates comprises: filtering the solid autologous platelet aggregates in a separator 406 to obtain filtered solid autologous platelet aggregates; extracting autologous concentrated growth factor gel from said filtered solid autologous platelet aggregates.

An autologous concentrated growth factor gel detection device 411 and an autologous concentrated growth factor gel extraction device 413 are arranged in the centrifugal device 401, wherein the autologous concentrated growth factor gel detection device 411 is used for detecting autologous concentrated growth factor gel from the solid autologous platelet aggregate after the centrifugal device 401 obtains the solid autologous platelet aggregate, and sending an autologous concentrated growth factor gel extraction instruction to the autologous concentrated growth factor gel extraction device 413 after the autologous concentrated growth factor gel is detected; the autologous concentrated growth factor gel extraction device 413 is configured to extract autologous concentrated growth factor gel from the solid autologous platelet aggregates according to the extraction instruction.

The autologous concentrated growth factor gel detection device 411 comprises a first image collector 414 and a first image analyzer 415; the first image collector 414 is configured to collect an image of a solid autologous platelet aggregate, and send the image of the solid autologous platelet aggregate to the image analyzer 415; the first image analyzer 415 is configured to locate the position of fibrin gel in the image of the solid autologous platelet aggregates, obtain location information, and send the location information to the autologous concentrated growth factor gel extraction device 413; the autologous concentrated growth factor gel extraction device 413 is configured to extract fibrin gel from the solid autologous platelet aggregates according to the positioning information, so as to obtain the autologous concentrated growth factor gel.

With reference to fig. 4, during the blood centrifugation process, the cover of the blood collection tube 419 is in a closed state, after the centrifugation process is completed, the cover of the blood collection tube 419 can be automatically opened, and the autologous concentrated growth factor gel extraction device 413 can extend into the blood collection tube 419 to extract the autologous concentrated growth factor gel. After the autologous concentrated growth factor gel is extracted by the autologous concentrated growth factor gel extraction device 413, the extracted autologous concentrated growth factor gel is transferred into the second shell 402 through the autologous concentrated growth factor gel transfer tube 409.

A disinfection device 404 is further arranged in the centrifugal device 401, and the disinfection device 404 is used for disinfecting equipment. In the operation of the sterilization treatment, ultraviolet sterilization or high-temperature and high-pressure sterilization may be used, and when ultraviolet sterilization is used, the ultraviolet sterilization is generally used for sterilizing devices that do not directly contact bone meal and blood. For example, the centrifugal device 401 may be subjected to ultraviolet sterilization, specifically ultraviolet sterilization. For the equipment directly contacting bone meal and blood and the equipment needing to be reused, the equipment is detachable non-disposable equipment, the disinfection mode adopts high-temperature high-pressure disinfection treatment, for example, the separator 406 is the equipment capable of separating the shell 400, and when the equipment is used, the detachable equipment needs to be subjected to high-temperature high-pressure disinfection treatment through the disinfection equipment 404.

S103: placing the autologous concentrated growth factor gel in a film pressing device for film pressing treatment to form a first autologous concentrated growth factor film;

in the embodiment of the application, the autologous concentrated growth factor gel is placed in a film pressing device for film pressing treatment, and the method specifically comprises the following steps: and taking the autologous concentrated growth factor gel out of the normal saline, placing the autologous concentrated growth factor gel in a membrane pressing device, extruding partial liquid, and carrying out membrane pressing treatment on the autologous concentrated growth factor gel by the membrane pressing device to form a first autologous concentrated growth factor membrane. When the first autologous concentrated growth factor membrane is used for the maxillary sinus internal lifting operation, the first autologous concentrated growth factor membrane is pressed into a flat shape, and the thickness of the first autologous concentrated growth factor membrane is 800 micrometers.

The steps of the method for forming the first self-concentrating growth factor membrane can be implemented by a device, please refer to fig. 4, wherein a saline injection port 405 for injecting saline into the second housing 402 is disposed on the first housing 400, and a shield, such as a cover, for preventing dust from entering the device can be disposed on the saline injection port 405 to match and seal with the saline injection port 405. A saline transfer pipe 407 for transferring saline is disposed between the saline injection port 405 and the saline inlet of the second housing 402; the saline is added into the second housing 402 through the saline injection port 405, the second housing 402 is provided with a saline storage 408, the saline is added into the saline storage 408 of the second housing 402 through the saline injection port 405, and the saline storage 408 is used for storing the first auto-concentration growth factor membrane.

The auto-concentrated growth factor gel is transferred to the saline reservoir 408 of the second housing 402 through the auto-concentrated growth factor gel transfer tube 409 for storage. A first membrane pressing device 420 is further arranged in the second housing 402, and the first membrane pressing device 421 is used for performing membrane pressing treatment on the autologous concentrated growth factor gel. Specifically, the autologous concentrated growth factor gel taken out of the physiological saline is placed in a first membrane pressing device 420 for membrane pressing treatment to form a first autologous concentrated growth factor membrane. When the first autologous concentrated growth factor membrane is used for lifting in the maxillary sinus, the first membrane pressing device presses the first autologous concentrated growth factor membrane into a flat shape, and the thickness of the first autologous concentrated growth factor membrane is 800 microns.

S104: pressing a plurality of the first autologous concentrated growth factor membranes into second autologous concentrated growth factor membranes.

It should be noted that in maxillary sinus lifting, the sinus floor mucosa is better protected by the inferior tectorial membrane, and the mucosa perforation caused by friction of bone particles is not easy to occur. Collagen membrane is frequently used clinically, with additional charges. The autologous concentrated growth factor membrane can replace a collagen membrane to be used under the sinus floor mucosa, has better fitting performance, contains growth factors and can promote tissue regeneration, but has the problems of limited size and incomplete coverage.

Therefore, the plurality of first autologous concentrated growth factor membranes are pressed into the second autologous concentrated growth factor membrane, so that the second autologous concentrated growth factor membrane becomes a complete large membrane which is not easy to separate, the area of the membrane is increased, and the membrane is more suitable for covering the maxillary sinus floor mucosa.

In specific implementation, different overlapping methods can be adopted according to the window size of the maxillary sinus. And when the second autologous concentrated growth factor membrane is used for the maxillary sinus external lifting operation, the plurality of first autologous concentrated growth factor membranes are pressed into the second autologous concentrated growth factor membrane. Specifically, during pressing, the edges of the plurality of first autologous concentrated growth factor membranes can be overlapped and pressed into a flat shape to serve as a second autologous concentrated growth factor membrane. For example, the number of the plurality of the first autologous concentrated growth factor membranes is 4, the edges of the 4 flat first autologous concentrated growth factor membranes are overlapped, and the whole autologous concentrated growth factor membrane is pressed together by using a pair of film pressing pliers to be used as the second autologous concentrated growth factor membrane, and the thickness of the second autologous concentrated growth factor membrane is 200 microns; or a plurality of the first self-concentration growth factor films can be mutually wound and pressed into a fan shape to be used as a second self-concentration growth factor film. In other words, the second autologous concentrated growth factor membrane is pressed into a fan shape by adopting a method of 'twist' type superposition, so as to ensure that the sinus floor mucosa is covered as much as possible.

The second autologous concentrated growth factor membrane is used for covering the inferior mucosa of the maxillary sinus, and the maxillary sinus is a perforated maxillary sinus; the pressing of the plurality of first autologous concentrated growth factor membranes into a second autologous concentrated growth factor membrane comprises: and pressing a plurality of first self-concentration growth factor films corresponding to the perforating positions, so that the thickness of the film at the perforating positions is thicker than that of the surrounding parts, and the second self-concentration growth factor films are used. The case is that the maxillary sinus has a perforation, and a plurality of blocks can be overlapped corresponding to the perforation position during film pressing, so that the maxillary sinus is thicker than the surrounding part.

The method steps of forming the second self-concentration growth factor membrane can be implemented by, with continued reference to fig. 4, providing a saline injection port 405 for injecting saline into the second housing 402 on the first housing 400, and providing a saline transfer tube 407 for transferring saline between the saline injection port 405 and the saline inlet of the second housing 402; wherein, a shelter, such as a cover, can be disposed on the saline injection port 405 to match the saline injection port 405 for preventing dust from entering the device.

The saline delivery tube 407 is a telescopic tube, when saline needs to be injected into the second housing 402, the saline delivery tube 407 is connected to the second housing 402, and when saline injection is completed, the saline delivery tube 407 and the second housing 402 can be disconnected at any time.

A physiological saline storage 408, a first membrane pressing device 420 and a second membrane pressing device 421 are arranged in the second shell 402; the physiological saline reservoir 408 is used for storing a first autologous concentrated growth factor membrane, the first membrane pressing device 420 is used for performing membrane pressing treatment on the autologous concentrated growth factor gel to form a first autologous concentrated growth factor membrane, and the second membrane pressing device 421 is used for performing membrane pressing treatment on a plurality of first autologous concentrated growth factor membranes to form a second autologous concentrated growth factor membrane.

An autologous concentrated growth factor gel conveying pipe 409 for conveying autologous concentrated growth factor gel is arranged between the centrifugal device 401 and the second shell 402; extracting autologous concentrated growth factor gel from the solid autologous platelet aggregates, and transferring the extracted autologous concentrated growth factor gel to the saline reservoir 408 of the second housing 402 through the autologous concentrated growth factor gel transfer tube 409 for storage and standby.

And taking the processed autologous concentrated growth factor gel out of the physiological saline reservoir 408, placing the autologous concentrated growth factor gel in a first membrane pressing device 420 for membrane pressing to form a first autologous concentrated growth factor membrane, and conveying a plurality of first autologous concentrated growth factor membranes into a second membrane pressing device 421 for membrane pressing to form a second autologous concentrated growth factor membrane.

When the first autologous concentrated growth factor membrane is used for the maxillary sinus internal lifting operation, the first membrane pressing device 420 presses the first autologous concentrated growth factor membrane into a flat shape, and the thickness of the first autologous concentrated growth factor membrane is 800 micrometers.

When the second autologous concentrated growth factor membrane is used for the maxillary sinus external lifting operation, the second membrane pressing device presses a plurality of the first autologous concentrated growth factor membranes into the second autologous concentrated growth factor membrane. Specifically, during pressing, the edges of the plurality of first autologous concentrated growth factor membranes can be overlapped and pressed into a flat shape to serve as a second autologous concentrated growth factor membrane. For example, the number of the plurality of first autologous concentrated growth factor membranes is 4, the 4 flat first autologous concentrated growth factor membranes are overlapped at the edges, the second membrane pressing device 421 presses the 4 flat first autologous concentrated growth factor membranes into a whole autologous concentrated growth factor membrane as the second autologous concentrated growth factor membrane, and the thickness of the second autologous concentrated growth factor membrane is 200 μm; or a plurality of the first autologous concentrated growth factor membranes can be mutually wound and pressed into a fan shape to be used as a second autologous concentrated growth factor membrane. In other words, the second membrane pressing device 421 presses the second autologous concentrated growth factor membrane into a fan shape by the method of "twist" type superposition, so as to ensure that the sinus floor mucosa is covered as much as possible.

If the maxillary sinus is a perforated maxillary sinus; the second film pressing device 421 presses the plurality of first autologous concentrated growth factor films arranged at the perforation positions, so that the thickness of the film at the perforation positions is thicker than that of the surrounding parts, and the second autologous concentrated growth factor films are obtained.

It should be noted that a transmission mechanism 410 for transmitting the second housing 402 to the outside of the first housing 400 is further disposed in the first housing 400. Referring to fig. 5, a second autologous concentrated growth factor membrane detection device 412 is disposed in the second housing 402; the second autologous concentrated growth factor membrane detection device 412 is configured to send a first transmission instruction for transmitting the second housing 402 out of the first housing 400 to the transmission mechanism 410 after detecting the second autologous concentrated growth factor membrane; the transmission mechanism 410 is specifically configured to transmit the second housing 402 out of the first housing 400 according to the first transmission instruction.

The second autologous concentrated growth factor membrane test device 412 comprises a second image collector 416 and a second image analyzer 417; the second image collector 416 is configured to collect an image in the second housing 402, and send the image in the second housing 402 to the second image analyzer 417; the second image analyzer 417 is configured to analyze the image in the second housing 402, and send the transmission instruction to the transmission mechanism 410 if the image of the second autologous condensed growth factor membrane is detected in the image in the second housing 402. The transmission mechanism 410 transmits the second housing 402 to the outside of the first housing 400 according to a first transmission instruction.

It should be noted that when the transmission mechanism 410 transmits the second housing 402 to the outside of the first housing 400, the second autologous concentrated growth factor membrane test device 412 and the second image collector 416 and the second image analyzer 417 inside the second autologous concentrated growth factor membrane test device 412 may be transmitted to the outside of the first housing 400 together with the second housing 402, or may remain in the first housing 400 without being moved with the second housing 402, which is within the protection scope of the embodiment of the present application.

The transmission mechanism 410 is further configured to transmit the second housing 402 into the first housing 400 after obtaining a second transmission instruction for transmitting the second housing 402 into the first housing 400.

A movable trigger button assembly 418 is arranged on the first shell 400 and used for triggering the second shell 402 to move; the moving trigger button assembly 418 is configured to determine a position of the second housing 402 in response to a trigger operation on the moving trigger button assembly 418, and send a second transmission instruction for transmitting the second housing 402 into the first housing 400 to the transmission mechanism 410 if the second housing 402 is outside the first housing 400.

A movable trigger button assembly 418 for triggering the second shell 402 to move is arranged on the first shell 400; the moving trigger button assembly 418 is configured to determine a position of the second housing 402 in response to a trigger operation on the moving trigger button assembly 418, and send a first transmission instruction for transmitting the second housing 402 out of the first housing 400 to the transmission mechanism 410 if the second housing 402 is inside the first housing 400.

The embodiment of the application also provides preparation equipment of the autologous concentrated growth factor gel, which comprises a first shell, a centrifugal device arranged in the first shell and a second shell arranged in the first shell;

a blood collection tube inlet for placing a blood collection tube filled with blood to be centrifuged into the centrifuge is formed in the first housing;

a normal saline injection port for injecting normal saline into the second shell is arranged on the first shell, and a normal saline conveying pipe for conveying normal saline is arranged between the normal saline injection port and the normal saline inlet of the second shell;

an autologous concentrated growth factor gel conveying pipe for conveying autologous concentrated growth factor gel is arranged between the centrifugal device and the second shell;

A physiological saline storage, a first membrane pressing device and a second membrane pressing device are arranged in the second shell;

the physiological saline storage is used for storing a first self-concentration growth factor film, the first film pressing device is used for performing film pressing treatment on the self-concentration growth factor gel to form a first self-concentration growth factor film, and the second film pressing device is used for performing film pressing treatment on a plurality of first self-concentration growth factor films to form a second self-concentration growth factor film;

wherein, a blood collection tube filled with blood to be centrifugally treated is placed into the centrifugal device through the blood collection tube placement opening, physiological saline is injected into the physiological saline storage of the second shell through the physiological saline conveying tube, the centrifugal device carries out centrifugal treatment on the blood to obtain solid autologous platelet aggregate, autologous concentrated growth factor gel is extracted from the solid autologous platelet aggregate, the extracted autologous concentrated growth factor gel is conveyed into the physiological saline storage of the second shell through the autologous concentrated growth factor gel conveying tube, the treated autologous concentrated growth factor gel is taken out from the physiological saline storage, the autologous concentrated growth factor gel is placed into a first film pressing device for film pressing treatment to form a first autologous concentrated growth factor film, and conveying the plurality of first self-concentration growth factor films into a second film pressing device for film pressing treatment to form second self-concentration growth factor films.

As shown in fig. 4, a centrifugation device 401, a second housing 402 disposed inside the first housing 400, and an autologous concentrated growth factor gel transfer tube 409 are disposed inside the first housing 400, wherein the autologous concentrated growth factor gel transfer tube 409 is disposed between the centrifugation device 401 and the second housing 402 for transferring autologous concentrated growth factor gel.

The first case 400 is provided with a blood collection tube insertion port 403 for inserting a blood collection tube into the centrifuge 401.

Specifically, in operation, a blood collection tube 419 containing blood to be centrifuged is placed into the centrifuge 401 through the blood collection tube inlet 403, the centrifuge 401 centrifuges the blood to obtain solid autologous platelet aggregates, and the autologous concentrated growth factor gel is extracted from the solid autologous platelet aggregates.

In addition, the method for extracting the autologous concentrated growth factor gel from the solid autologous platelet aggregate comprises the following steps: filtering the solid autologous platelet aggregates in a separator to obtain filtered solid autologous platelet aggregates; extracting autologous concentrated growth factor gel from said filtered solid autologous platelet aggregates. With continued reference to fig. 4, the centrifugation device 401 centrifuges the blood, specifically, the blood collection tube 419 is placed into the centrifugation device 401 for centrifugation, after centrifugation, the solid autologous platelet aggregates are placed into the separator 406, and a part of the liquid is filtered out, so as to obtain the filtered solid autologous platelet aggregates. The autologous concentrated growth factor gel extraction device 413 extracts autologous concentrated growth factor gel from the filtered solid autologous platelet aggregates.

The extraction process of the autologous concentrated growth factor gel is described in further detail below.

An autologous concentrated growth factor gel detection device 411 and an autologous concentrated growth factor gel extraction device 413 are arranged in the centrifugal device 401, wherein the autologous concentrated growth factor gel detection device 411 is used for detecting autologous concentrated growth factor gel from the solid autologous platelet aggregate after the centrifugal device 401 obtains the solid autologous platelet aggregate, and sending an autologous concentrated growth factor gel extraction instruction to the autologous concentrated growth factor gel extraction device 413 after the autologous concentrated growth factor gel is detected; the autologous concentrated growth factor gel extraction device 413 is used for extracting autologous concentrated growth factor gel from the solid autologous platelet aggregate according to the extraction instruction.

It should be noted that the autologous concentrated growth factor gel detection apparatus 411 includes a first image collector 414 and a first image analyzer 415; the first image collector 414 is configured to collect an image of a solid autologous platelet aggregate, and send the image of the solid autologous platelet aggregate to the image analyzer; the first image analyzer 415 is configured to locate the position of fibrin gel in the image of the solid autologous platelet aggregates, obtain location information, and send the location information to the autologous concentrated growth factor gel extraction device 413; the autologous concentrated growth factor gel extraction device 413 is configured to extract fibrin gel from the solid autologous platelet aggregates according to the positioning information, so as to obtain the autologous concentrated growth factor gel. From here, the autologous concentrated growth factor gel extraction was completed.

After the extraction of the autologous concentrated growth factor gel is completed, the extracted autologous concentrated growth factor gel is transferred into the second shell 402 through the autologous concentrated growth factor gel transfer tube 409, and then the next stage is performed: preparation of a first autologous concentrated growth factor membrane.

A saline injection port 405 for injecting saline into the second housing 402 is provided on the first housing 400, and a saline transfer tube 407 for transferring saline is provided between the saline injection port 405 and a saline inlet of the second housing 402; the saline conveying pipe 407 is a telescopic pipe, when the saline needs to be injected into the second housing 402, the saline conveying pipe 407 is connected with the second housing 402, and after the saline injection is completed, the saline conveying pipe 407 and the second housing 402 can be disconnected at any time.

A physiological saline storage 408, a first membrane pressing device 420 and a second membrane pressing device 421 are arranged in the second shell 402; the physiological saline reservoir 408 is used for storing a first autologous concentrated growth factor membrane, and the first membrane pressing device 420 is used for performing membrane pressing treatment on the autologous concentrated growth factor gel to form a first autologous concentrated growth factor membrane. Specifically, autologous concentrated growth factor gel is extracted from the solid autologous platelet aggregate, the extracted autologous concentrated growth factor gel is transferred to the physiological saline reservoir 408 of the second housing 402 through the autologous concentrated growth factor gel transfer tube 409, the treated autologous concentrated growth factor gel is taken out from the physiological saline reservoir 408, and the autologous concentrated growth factor gel is placed in a first membrane pressing device 420 for membrane pressing treatment to form a first autologous concentrated growth factor membrane. When the first autologous concentrated growth factor membrane is used for the maxillary sinus internal lifting operation, the first membrane pressing device 420 presses the first autologous concentrated growth factor membrane into a flat shape.

After the first autologous concentrated growth factor membrane is formed, the following steps are performed: and preparing a second autologous concentrated growth factor membrane.

And conveying a plurality of the first self-concentration growth factor films into a second film pressing device for film pressing treatment to form second self-concentration growth factor films. Referring to fig. 4, different squeeze films are used according to the size of the maxillary sinus window. When the second autologous concentrated growth factor membrane is used for the maxillary sinus external lifting operation, the second membrane pressing device 421 presses the plurality of first autologous concentrated growth factor membranes into the second autologous concentrated growth factor membrane. Specifically, during pressing, the edges of the plurality of first autologous concentrated growth factor membranes can be overlapped and pressed into a flat shape to serve as a second autologous concentrated growth factor membrane. For example, the number of the plurality of the first autologous concentrated growth factor membranes is 4, the edges of the 4 flat first autologous concentrated growth factor membranes are overlapped, and the whole autologous concentrated growth factor membrane is pressed together by using a pair of film pressing pliers to be used as the second autologous concentrated growth factor membrane, and the thickness of the second autologous concentrated growth factor membrane is 200 microns; or a plurality of the first autologous concentrated growth factor membranes can be mutually wound and pressed into a fan shape to be used as a second autologous concentrated growth factor membrane. In other words, the second autologous concentrated growth factor membrane is pressed into a fan shape by adopting a method of 'twist' type superposition, so as to ensure that the sinus floor mucosa is covered as much as possible.

In the embodiment of the present application, as shown in fig. 5, a transmission mechanism 410 for transmitting the second housing 402 to the outside of the first housing 400 is further disposed in the first housing 400. It should be noted that a second self-concentration growth factor membrane detection device 412 is disposed in the second housing 402; the second autologous concentrated growth factor membrane detection device 412 is configured to send a first transmission instruction for transmitting the second housing 402 out of the first housing 400 to the transmission mechanism 410 after detecting the second autologous concentrated growth factor membrane; the transmission mechanism 410 is specifically configured to transmit the second housing 402 out of the first housing 400 according to the first transmission instruction.

After detecting the second autologous concentrated growth factor membrane, the second autologous concentrated growth factor membrane detection device 412 sends a first transmission instruction to the transmission mechanism 410 to transmit the second housing 402 to the outside of the first housing 400, at this time, the saline transmission tube 407 contracts, that is, the saline transmission tube 407 is disconnected from the second housing 402, and then the transmission mechanism 410 transmits the second housing 402 to the outside of the first housing 400 according to the first transmission instruction. After the second housing 402 is transferred to the outside of the first housing 400, the second housing 402 may be opened, or the second autologous concentrated growth factor membrane may be ejected from the second housing 402 in other ways, which is not specifically limited herein, to obtain the second autologous concentrated growth factor membrane.

It should be noted that the second autologous concentrated growth factor membrane detection device 412 includes a second image collector 416 and a second image analyzer 417; the second image collector 416 is configured to collect an image in the second housing 402, and send the image in the second housing 402 to the second image analyzer 417; the second image analyzer 417 is configured to analyze the image in the second housing 402, and send the transmission instruction to the transmission mechanism 410 if the image of the second autologous condensed growth factor membrane is detected in the image in the second housing 402. The transmission mechanism 410 transmits the second housing 402 to the outside of the first housing 400 according to a first transmission instruction.

In the embodiment of the present application, the transmission mechanism 410 is further configured to transmit the second housing 402 into the first housing 400 after obtaining a second transmission instruction for transmitting the second housing 402 into the first housing 400. It should be noted that, when the transmission mechanism 410 receives a first transmission command for transmitting the second housing 402 out of the first housing 400, the autologous concentrated growth factor gel transmission tube 409 is disconnected from the second housing 402; when the transmission mechanism 410 receives a second transmission instruction for transmitting the second housing 402 into the first housing 400, the autologous concentrated growth factor gel transmission tube 409 is connected with the second housing 402.

In the embodiment of the present application, a moving trigger button assembly 418 for triggering the second housing 402 to move is disposed on the first housing 400; the moving trigger button assembly 418 is configured to determine a position of the second housing 402 in response to a trigger operation on the moving trigger button assembly 418, and send a second transmission instruction for transmitting the second housing 402 into the first housing 400 to the transmission mechanism 410 if the second housing 402 is outside the first housing 400.

Although the present application has been described with reference to the preferred embodiments, it is not intended to limit the present application, and those skilled in the art can make variations and modifications without departing from the spirit and scope of the present application, therefore, the scope of the present application should be determined by the claims that follow.

In a typical configuration, a computing device includes one or more processors (CPUs), input/output interfaces, network interfaces, and memory.

The memory may include forms of volatile memory in a computer readable medium, Random Access Memory (RAM) and/or non-volatile memory, such as Read Only Memory (ROM) or flash memory (flash RAM). Memory is an example of a computer-readable medium.

Computer-readable media, including both non-transitory and non-transitory, removable and non-removable media, may implement information storage by any method or technology. The information may be computer readable instructions, data structures, modules of a program, or other data. Examples of computer storage media include, but are not limited to, phase change memory (PRAM), Static Random Access Memory (SRAM), Dynamic Random Access Memory (DRAM), other types of Random Access Memory (RAM), Read Only Memory (ROM), Electrically Erasable Programmable Read Only Memory (EEPROM), flash memory or other memory technology, compact disc read only memory (CD-ROM), Digital Versatile Discs (DVD) or other optical storage, magnetic cassettes, magnetic tape magnetic disk storage or other magnetic storage devices, or any other non-transmission medium that can be used to store information that can be accessed by a computing device. As defined herein, computer readable media does not include non-transitory computer readable media (transient media), such as modulated data signals and carrier waves.

Claims

1. The preparation method of the autologous concentrated growth factor membrane is characterized by comprising the following steps of:

putting the blood to be centrifuged into a centrifuge for blood centrifugation to obtain a solid autologous platelet aggregate;

extracting autologous concentrated growth factor gel from the solid autologous platelet aggregates;

placing the autologous concentrated growth factor gel in a film pressing device for film pressing treatment to form a first autologous concentrated growth factor film;

pressing a plurality of the first autologous concentrated growth factor membranes into second autologous concentrated growth factor membranes;

wherein the second autologous concentrated growth factor membrane is used for covering the inframucosal membrane of the maxillary sinus, and the maxillary sinus is a perforated maxillary sinus;

2. The method for preparing an autologous concentrated growth factor membrane according to claim 1, wherein the solid autologous platelet aggregate comprises: serum, fibrin gel, and red blood cell layer.

3. The method for preparing an autologous concentrated growth factor membrane according to claim 1, wherein the step of extracting the autologous concentrated growth factor gel from the solid autologous platelet aggregates comprises:

4. The method for preparing the autologous concentrated growth factor membrane according to claim 1, wherein the step of placing the autologous concentrated growth factor gel in a membrane pressing device for membrane pressing treatment to form a first autologous concentrated growth factor membrane comprises: and placing the autologous concentrated growth factor gel taken out from the physiological saline into a film pressing device for film pressing treatment to form a first autologous concentrated growth factor film.

5. The method for preparing an autologous concentrated growth factor membrane according to claim 1, wherein the first autologous concentrated growth factor membrane is pressed into a flat shape when being used in the maxillary sinus lifting operation.

6. The method for preparing an autologous concentrated growth factor membrane according to claim 5, wherein the thickness of the first autologous concentrated growth factor membrane is 800 μm.

7. The method for preparing an autologous concentrated growth factor membrane according to claim 1, wherein the second autologous concentrated growth factor membrane is prepared by pressing a plurality of first autologous concentrated growth factor membranes into a second autologous concentrated growth factor membrane when the second autologous concentrated growth factor membrane is used for the maxillary sinus external lifting operation.

8. The method for preparing an autologous concentrated growth factor membrane according to claim 1, further comprising: venous blood of the patient is drawn as the blood to be centrifuged.

9. The method for preparing an autologous concentrated growth factor membrane according to claim 7, wherein the number of the first autologous concentrated growth factor membranes is 4.

10. The method for preparing an autologous concentrated growth factor membrane according to claim 7, wherein the thickness of the second autologous concentrated growth factor membrane is 200 μm.

11. The preparation equipment of the autologous concentrated growth factor membrane is characterized by comprising a first shell, a centrifugal device arranged in the first shell, and a second shell arranged in the first shell;

A blood collection tube injection port for inserting a blood collection tube containing blood to be centrifuged into the centrifuge is provided in the first case;

a physiological saline storage device, a first membrane pressing device and a second membrane pressing device are arranged in the second shell;

12. The apparatus for preparing as claimed in claim 11 wherein said extracting autologous concentrated growth factor gel from said solid autologous platelet aggregate comprises:

13. The preparation equipment according to claim 11, wherein an autologous concentrated growth factor gel detection device and an autologous concentrated growth factor gel extraction device are arranged in the centrifugation device;

14. The preparation apparatus according to claim 13, wherein the autologous concentrated growth factor gel detection device comprises a first image collector and a first image analyzer;

the autologous concentrated growth factor gel extraction device extracts fibrin gel from the solid autologous platelet aggregates according to the positioning information.

15. The manufacturing apparatus according to claim 11, wherein a transmission mechanism for transferring the second housing outside the first housing is further provided in the first housing;

16. The manufacturing apparatus as set forth in claim 11 wherein said first autologous concentrated growth factor membrane is compressed into a flat shape by said first compressor when said first autologous concentrated growth factor membrane is used in an maxillary sinus lifting procedure.

17. The apparatus for preparing according to claim 11, wherein the second self-concentrating growth factor membrane is used for an external maxillary sinus lifting operation, and the second membrane pressing device presses the plurality of first self-concentrating growth factor membranes into the second self-concentrating growth factor membrane.

18. The manufacturing apparatus according to claim 11, wherein the second autologous concentrated growth factor membrane is used to cover the inframucosal membrane of the maxillary sinus if the maxillary sinus is a perforated maxillary sinus;

19. The preparation apparatus according to claim 15, wherein a second autologous concentrated growth factor membrane detection device is disposed in the second housing;

20. The preparation apparatus according to claim 19, wherein the second autologous concentrated growth factor membrane detection device comprises a second image collector and a second image analyzer;

21. The manufacturing apparatus according to claim 15, wherein the transmission mechanism is further configured to transfer the second housing into the first housing after obtaining a second transfer instruction to transfer the second housing into the first housing.

22. The manufacturing apparatus of claim 21, wherein a movement activation button assembly is provided on the first housing for activating movement of the second housing;

23. The manufacturing apparatus of claim 15, wherein a movement activation button assembly is provided on the first housing for activating movement of the second housing;