CN113796440A

CN113796440A - Soluble snake gall chrysanthemum tea and preparation method thereof

Info

Publication number: CN113796440A
Application number: CN202111091785.4A
Authority: CN
Inventors: 曾晓艳; 刘塔斯; 谭朝阳; 刘广锐; 龙俊鹏
Original assignee: Hunan University of Chinese Medicine
Current assignee: Hunan University of Chinese Medicine
Priority date: 2021-09-17
Filing date: 2021-09-17
Publication date: 2021-12-17

Abstract

The invention discloses soluble snake gall chrysanthemum tea and a preparation method thereof, wherein the snake gall chrysanthemum tea comprises the following raw materials in parts by mass: 0.5-2 parts of snake gall, 10-30 parts of chrysanthemum, 30-50 parts of dried orange peel, 5-15 parts of frosted mulberry leaf and 5-15 parts of liquorice; the manufacturing method comprises the following steps: cutting fel Serpentis, dissolving in water, stirring, filtering to obtain fel Serpentis extractive solution, and refrigerating; soaking pericarpium Citri Tangerinae, flos Chrysanthemi, folium Mori, and Glycyrrhrizae radix in water for the first time, reflux-decocting, filtering, adding water to the residue, reflux-decocting for the second time, filtering, mixing filtrates, evaporating, and concentrating to obtain Chinese medicinal extract; mixing the Chinese medicinal extract and fel Serpentis extractive solution, evaporating to concentrate into soft extract, adding sucrose dextrin, adding Mentholum, mixing, granulating, drying, and packaging to obtain fel Serpentis flos Chrysanthemi tea; the invention not only keeps the efficacy of the snake gall, but also neutralizes the bitter taste of the snake gall by the sweet taste of the liquorice; meanwhile, the tea has good health care and prevention effects on diseases of ophthalmology and lung system; it is made into granule, and is convenient for carrying and administration.

Description

Soluble snake gall chrysanthemum tea and preparation method thereof

Technical Field

The invention relates to the technical field of functional tea, in particular to soluble snake gall chrysanthemum tea and a preparation method thereof.

Background

The snake gall has good effects of clearing liver and improving vision, dispelling wind and removing dampness, and clearing heat and removing toxicity, and is especially suitable for people with myopia, dry eyes and fatigue due to long time for modern people to watch computers and mobile phones. The snake gall is relatively expensive, is directly taken after soaking, has strong fishy and bitter tastes, and is not easy to absorb effective components of the snake gall by a human body, so the snake gall is extracted by a water extraction method; the snake gall has few finished products, and the market mainly comprises snake gall wine, Chinese patent medicines, dried snake gall, bile vacuum dry powder, snake gall pills and the like. Although there are many finished products of the snake gall tea, the raw medicinal materials of the snake gall tea are single in component, single in curative effect and relatively high in cost, and the snake gall tea is directly drunk or taken with water, has heavy fishy and bitter tastes and is unacceptable to consumers.

The prior art of using snake gall to make tea has the patent numbers: 201710608926.2 patent, wherein the snake gall is directly punctured and dropped on the jasmine Pu 'er tea, then the drying and packaging are carried out, the taste is fishy and can not be accepted by most people, and the jasmine Pu' er snake gall tea has residues after being brewed. It is not very comfortable to take.

The product adopts the formula of the snake gall and 4 edible traditional Chinese medicinal materials, is prepared into soluble granules by scientific extraction, has the effects of clearing away lung-heat, moistening dryness, relieving cough, reducing phlegm, clearing liver and improving vision, and has good health-care and prevention and treatment effects on ophthalmic and lung system diseases.

Disclosure of Invention

The invention aims to provide soluble snake gall chrysanthemum tea and a preparation method thereof, and solves the problems of fishy smell, better effect and inconvenience in drinking of the snake gall tea.

The invention is realized in such a way that the soluble snake gall chrysanthemum tea comprises the raw materials of snake gall, chrysanthemum, dried orange peel, frosted mulberry leaf and liquorice; the raw materials comprise the following components in parts by mass: 0.5-2 parts of snake gall, 10-30 parts of chrysanthemum, 30-50 parts of dried orange peel, 5-15 parts of frosted mulberry leaf and 5-15 parts of liquorice.

The further technical scheme of the invention is as follows: the raw materials comprise, by mass, 1 part of snake gall, 20 parts of chrysanthemum, 40 parts of dried orange peel, 10 parts of frosted mulberry leaf and 10 parts of liquorice.

The further technical scheme of the invention is as follows: the raw materials also comprise 0.05-0.1 part of menthol by mass.

A method for preparing soluble snake gall chrysanthemum tea comprises the following steps:

cutting fel Serpentis, adding 8-12 times of water to dissolve, stirring in water bath until fel Serpentis is completely dissolved, filtering to obtain fel Serpentis extractive solution, and refrigerating in refrigerator;

step two, processing dried orange peel, chrysanthemum, frosted mulberry leaf and liquorice, adding 8-12 times of water, carrying out reflux decoction after first soaking, filtering, adding 6-10 times of water into dregs of a decoction, carrying out reflux decoction for the second time, filtering, combining 2 times of filtrates, evaporating and concentrating to obtain a traditional Chinese medicine extract;

and step three, combining the traditional Chinese medicine extract prepared in the step two and the snake gall extracting solution prepared in the step one, evaporating and concentrating the mixture into thick paste, weighing the thick paste to calculate the weight of the thick paste, adding 3-5 times of sucrose dextrin, adding menthol, uniformly mixing, granulating, drying for 1-3 hours, and packaging to obtain the soluble snake gall chrysanthemum tea.

The further technical scheme of the invention is as follows: the temperature of the water bath in the first step is 50-80 ℃.

The further technical scheme of the invention is as follows: in the second step, the reflux decoction time after the first soaking is as follows: soaking for 20-40 minutes, and carrying out reflux decoction for 2-3 hours, wherein the second reflux decoction time is as follows: decocting under reflux for 1-2 hr.

The further technical scheme of the invention is as follows: the mass ratio of the sucrose dextrin in the third step is 1-5: 1-7.

The further technical scheme of the invention is as follows: 1g of snake gall in the first step, 20g of chrysanthemum, 40g of dried orange peel, 10g of frosted mulberry leaf and 10g of liquorice in the second step, and 0.08g of menthol in the third step.

The snake gall, the mulberry leaves and the chrysanthemum are cold in nature and sweet and bitter in taste, the snake gall has the effects of dispelling wind and eliminating dampness, clearing liver and improving eyesight and detoxifying, the mulberry leaves dispel wind and heat, clearing lung and moistening dryness, clearing liver and improving eyesight, the chrysanthemum has the effects of dispelling wind and clearing heat, calming liver and improving eyesight, and the mulberry leaves and the chrysanthemum have good effects of clearing liver and improving eyesight after entering lung and liver channels; the dried orange peel is warm in nature, pungent and bitter in taste, enters spleen and lung channels, and has the effects of regulating qi, tonifying spleen, eliminating dampness and reducing phlegm; the liquorice has sweet taste, and has the effects of invigorating spleen and replenishing qi, eliminating phlegm and stopping cough, relieving spasm and pain and harmonizing the medicines; the snake gall has certain bitter taste, integrates the effects of other 4 traditional Chinese medicines, is blended by adding the liquorice, not only keeps the medicinal property of the snake gall, but also has the sweet taste of the liquorice.

The invention has the beneficial effects that: the snake gall chrysanthemum tea inherits the medicine-food homologous theory of the traditional Chinese medicine, mainly takes snake gall as a raw material, is supplemented with chrysanthemum and dried orange peel, and is combined with frosted mulberry leaf and liquorice to play the effects of synergy and complementary advantages, so that the efficacy of the snake gall chrysanthemum tea is exerted to the maximum; the novel snake gall chrysanthemum tea not only keeps the efficacy of snake gall, but also neutralizes the bitter and fishy taste of the snake gall by the sweet taste of the liquorice, integrates the efficacy of the above medicines, meets the requirements of people of different levels and different ages on the taste, is convenient to take, and is particularly required for the health care and prevention of diseases of ophthalmology and lung systems.

Detailed Description

The snake gall chrysanthemum tea mainly takes snake gall as a raw material, is supplemented with chrysanthemum and dried orange peel, is combined with frosted mulberry leaf and liquorice, has a synergistic effect and a complementary advantage, and exerts the effect of the snake gall chrysanthemum tea to the maximum. The method comprises the following specific steps:

a soluble snake gall chrysanthemum tea is prepared from snake gall, chrysanthemum, dried orange peel, frosted mulberry leaf and liquorice; the raw materials comprise the following components in parts by mass: 0.5-2 parts of snake gall, 10-30 parts of chrysanthemum, 30-50 parts of dried orange peel, 5-15 parts of frosted mulberry leaf and 5-15 parts of liquorice.

The raw materials comprise, by mass, 1 part of snake gall, 20 parts of chrysanthemum, 40 parts of dried orange peel, 10 parts of frosted mulberry leaf and 10 parts of liquorice.

The raw materials also comprise 0.05-0.1 part of menthol by mass.

The temperature of the water bath in the first step is 50-80 ℃.

In the second step, the reflux decoction time after the first soaking is as follows: soaking for 20-40 minutes, and carrying out reflux decoction for 2-3 hours, wherein the second reflux decoction time is as follows: decocting under reflux for 1-2 hr.

The mass ratio of the sucrose dextrin in the third step is 1-5: 1-7.

1g of snake gall in the first step, 20g of chrysanthemum, 40g of dried orange peel, 10g of frosted mulberry leaf and 10g of liquorice in the second step, and 0.08g of menthol in the third step.

In order to make the above embodiments of the present invention more understandable to those skilled in the art, a specific experiment is described below.

The first embodiment is as follows:

the embodiment discloses a method for preparing soluble snake gall chrysanthemum tea, which comprises the steps of cutting 1g of snake gall into pieces, adding 10 times of water to dissolve the snake gall into the water, continuously stirring the mixture in water bath at 60 ℃ until the snake gall is completely dissolved, filtering the mixture to obtain snake gall extracting solution, and placing the snake gall extracting solution into a refrigerator for refrigeration for later use; slightly processing 40g of dried orange peel, 20g of chrysanthemum, 10g of frosted mulberry leaf and 10g of liquorice, adding 10 times of water, soaking for 30 minutes, then carrying out reflux decoction for 2 hours, filtering, adding 8 times of water into dregs of a decoction, carrying out reflux decoction for 1 hour, filtering, combining 2 times of filtrates, evaporating and concentrating to obtain a traditional Chinese medicine extract; mixing the Chinese medicinal extract and fel Serpentis extractive solution, evaporating to obtain soft extract, weighing, calculating the weight of the soft extract, adding 4 times of sucrose dextrin (the ratio of sucrose to dextrin is 1:2), mixing, granulating with distilled water as wetting agent, drying for 2 hr, and packaging to obtain fel Serpentis tea.

Example two:

the embodiment discloses a method for preparing soluble snake gall chrysanthemum tea, which comprises the steps of cutting 1g of snake gall into pieces, adding 10 times of water to dissolve the snake gall into the water, continuously stirring the mixture in water bath at 60 ℃ until the snake gall is completely dissolved, filtering the mixture to obtain snake gall extracting solution, and placing the snake gall extracting solution into a refrigerator for refrigeration for later use; slightly processing 40g of dried orange peel, 20g of chrysanthemum, 10g of frosted mulberry leaf and 10g of liquorice, adding 10 times of water, soaking for 30 minutes, then carrying out reflux decoction for 2 hours, filtering, adding 8 times of water into dregs of a decoction, carrying out reflux decoction for 1 hour, filtering, combining 2 times of filtrates, evaporating and concentrating to obtain a traditional Chinese medicine extract; mixing the Chinese medicinal extract and fel Serpentis extractive solution, evaporating to obtain soft extract, weighing, calculating the weight of the soft extract, adding 4 times of sucrose dextrin (the ratio of sucrose to dextrin is 5:7), mixing, granulating with 80% ethanol as wetting agent, drying for 2 hr, and packaging.

Example three:

the embodiment discloses a method for preparing soluble snake gall chrysanthemum tea, which comprises the steps of cutting 1g of snake gall into pieces, adding 10 times of water to dissolve the snake gall into the water, continuously stirring the mixture in water bath at 60 ℃ until the snake gall is completely dissolved, filtering the mixture to obtain snake gall extracting solution, and placing the snake gall extracting solution into a refrigerator for refrigeration for later use; slightly processing 40g of dried orange peel, 20g of chrysanthemum, 10g of frosted mulberry leaf and 10g of liquorice, adding 10 times of water, soaking for 30 minutes, then carrying out reflux decoction for 2 hours, filtering, adding 8 times of water into dregs of a decoction, carrying out reflux decoction for 1 hour, filtering, combining 2 times of filtrates, evaporating and concentrating to obtain a traditional Chinese medicine extract; mixing the Chinese medicinal extract and fel Serpentis extractive solution, evaporating to obtain soft extract, weighing, calculating the weight of the soft extract, adding 4 times of sucrose dextrin (the ratio of sucrose to dextrin is 2:3), mixing, granulating with 90% ethanol as wetting agent, drying for 2 hr, and packaging.

Example four:

the embodiment discloses a method for preparing soluble snake gall chrysanthemum tea, which comprises the steps of cutting 1g of snake gall into pieces, adding 10 times of water to dissolve the snake gall into the water, continuously stirring the mixture in water bath at 60 ℃ until the snake gall is completely dissolved, filtering the mixture to obtain snake gall extracting solution, and placing the snake gall extracting solution into a refrigerator for refrigeration for later use; slightly processing 40g of dried orange peel, 20g of chrysanthemum, 10g of frosted mulberry leaf and 10g of liquorice, adding 10 times of water, soaking for 30 minutes, then carrying out reflux decoction for 2 hours, filtering, adding 8 times of water into dregs of a decoction, carrying out reflux decoction for 1 hour, filtering, combining 2 times of filtrates, evaporating and concentrating to obtain a traditional Chinese medicine extract; mixing the Chinese medicinal extract and fel Serpentis extractive solution, evaporating to obtain soft extract, weighing, adding 4 times of sucrose dextrin (ratio of sucrose to dextrin is 1:1) and 0.1% of Mentholum, mixing, granulating, drying for 2 hr, and packaging.

Comparative example:

the embodiment discloses a preparation method of soluble snake gall chrysanthemum tea, which comprises the steps of cutting 1g of snake gall into pieces, adding 10 times of water to dissolve the pieces, stirring continuously in water bath at 60 ℃ until the snake gall is completely dissolved, filtering, evaporating and concentrating the obtained extracting solution to obtain thick paste, weighing and calculating the weight of the thick paste, adding 4 times of sucrose dextrin (the ratio of sucrose to dextrin is 1:1), mixing uniformly, granulating by using distilled water as a wetting agent, drying for 2 hours, and packaging to obtain the snake gall chrysanthemum tea.

And (3) detection results:

the snake gall tea is prepared by taking 3 parts of the granules of the example IV according to the moisture measurement operation (general rule 0832) under the item of pharmacopoeia 2020 edition, measuring the moisture of 2g each part, wherein the moisture measurement results are respectively 5.47%, 5.81% and 5.52%, and are all lower than 8%, the moisture measurement results accord with the moisture regulation of the pharmacopoeia on the traditional Chinese medicine granules, and the results are shown in the following table 1:

TABLE 1 moisture content results for Snake bile granules

The mouthfeel, solubility odor and appearance of the granules obtained in examples one to four and comparative example are compared as shown in table 2:

TABLE 2 comparison of Snake bile granules

Example five:

pharmacological experiments:

the snake gall chrysanthemum tea in the embodiment can be suitable for people of various ages, and has a good curative effect on common pharyngitis and bronchitis.

In order to further test the medicine property of the snake gall chrysanthemum tea, white mice are selected, the white mice are made to be test bodies through human factors, and are respectively subjected to cough relieving and phlegm reducing experiments, the number of the white mice in each experiment is 60, the number of the mice is half that of the mice in each experiment, the mice are randomly divided into 6 groups, and each group is ten. The experimental results obtained were as follows:

relieving cough:

the cough relieving effect of the snake gall chrysanthemum tea prepared by the invention is evaluated by a strong ammonia water spraying method. The mice are divided into 6 groups, namely a physiological saline group, a positive drug control group (compound liquorice oral solution 6ml/kg), and the first, second, third and fourth examples of the drug, the continuous gavage administration is carried out for 3d, after the 3d administration is carried out for 40min, the mice are placed in a 1000ml sealed glass container, and ammonia water 1: 7,3s arouses the white mouse to cough, takes the white mouse to have abdominal muscle contraction, chest contraction and mouth opening (cough sound is available or not) at the same time as the cough standard, and records the cough induction incubation period and the cough frequency of the white mouse within 5 min. The first, second, third and fourth examples can obviously prolong the cough latency of the mice, obviously reduce the cough frequency, have statistical significance in comparison with the blank control group, and have a certain dose-effect relationship, which indicates that the snake gall chrysanthemum tea has a better cough relieving effect.

Reducing phlegm:

the phlegm reducing effect of the snake gall chrysanthemum tea is evaluated by observing the influence of the snake gall chrysanthemum tea on the phenol red discharge amount of mice. The mice are divided into 6 groups, namely the first example, the second example, the third example and the fourth example, and a positive drug control group: the Mushutan oral liquid group (10g/kg) and the blank control group are both administered by one-time intragastric administration, and the control group is administered with distilled water with equal volume. After 1h of administration, 5% phenol red physiological saline solution is respectively injected into the abdominal cavity of the white mouse, the injection volume is 0.5ml per 20g of body weight, the white mouse is killed by cervical dislocation after 30min of phenol red injection, the trachea is separated, the syringe is inserted, the white mouse is fixed by ligation, the trachea and the bronchus of the white mouse are irrigated by 5% sodium bicarbonate for 4 times and 0.5ml each time, lavage liquid is collected, the absorbance is measured at the position of 546nm wavelength by a 722 type spectrophotometer, the phenol red discharge amount of each white mouse is calculated according to a standard curve, and comparison between groups is carried out. The results show that: the first, second, third and fourth examples of the snake gall chrysanthemum tea can increase the output of the mouse phenol red, have statistical significance compared with a blank control group, and are in a certain dose-effect relationship, so that the snake gall chrysanthemum tea has a good phlegm eliminating effect.

Example six:

the clinical verification of the snake gall chrysanthemum tea in the first embodiment is as follows: selecting patients with cough of lung-heat syndrome caused by acute tracheobronchitis, patients with disease course within 3 days, patients with no fever or mild-moderate fever and age of 20-65 years, adopting a random single-blind control test method, and adopting a simple random method for case grouping.

Selection of control group: the dripping pill is orally administered 2 granules each time 3 times a day.

Selection of treatment groups: the snake gall chrysanthemum tea in the first embodiment is selected and taken orally by flushing, one bag is used every time, 10g is used every time, and 3 times are taken every day.

The course of treatment is as follows: 7 days

Observation items and indexes:

1. safety observation

Clinically, adverse reaction symptoms such as diarrhea, abdominal pain, nausea, vomiting, rash and the like should be closely observed. Urinary routine, blood routine, fecal routine, electrocardiogram, liver function, kidney function;

2. curative effect observation item

Symptoms and scoring criteria, major clinical symptoms grouping:

the clinical cure is as follows: cough, expectoration, fever disappeared (down to grade 0), and other complications were either improved significantly (down to grade 2) or disappeared.

The effect is shown: cough, expectoration and fever are obviously better rotated (2 grades are reduced), and other complications are better rotated (1 grade is reduced)

The method has the following advantages: cough, expectoration and fever are improved (1 grade is reduced), and other complications are improved (1 grade is reduced)

And (4) invalidation: none of the symptoms improved or worsened.

Statistical processing and data summarization.

The statistical method comprises the following steps: classification data were examined by X2, ranking data were examined by two-sample Wilcoxon rank-sum test (calibration), and mean comparison of two samples was examined by t test.

As a result: the total number of the subjects was 100, 50 in the treatment group and 50 in the control group, and there was no significant difference in age distribution, disease course and sex. The western medicine diagnoses acute tracheo-bronchitis. The syndrome differentiation of the traditional Chinese medicine belongs to the syndrome of phlegm-heat cough.

And (3) comparing the curative effects:

compared with the two groups, the treatment group has significant difference compared with the control group, and the curative effect is better.

The snake gall, the mulberry leaves and the chrysanthemum are cold in nature and sweet and bitter in taste, the snake gall has the effects of dispelling wind and removing dampness, clearing liver and improving eyesight and detoxifying, the mulberry leaves and the chrysanthemum have better effects of clearing liver and improving eyesight when entering lung and liver channels, and the chrysanthemum also has the effects of clearing heat and detoxifying; the dried orange peel is warm in nature, pungent and bitter in taste, enters spleen and lung channels, and has the effects of regulating qi, tonifying spleen, eliminating dampness and reducing phlegm; the snake gall chrysanthemum tea has certain bitter taste, and the liquorice is added for seasoning, so that the medicinal properties of the snake gall are maintained, the sweetness of the liquorice is also achieved, the effects of other four traditional Chinese medicines are integrated, and the effects of monarch, minister, assistant and guide are cooperated with each other to assist and guide, so that the effect of the snake gall chrysanthemum tea is exerted to the maximum; meanwhile, the menthol is added in the invention to increase the cool and refreshing inhalation feeling and reduce the bitter and fishy smell of the snake gall, and the soluble granular preparation can be prepared into a soluble granular preparation which can be accepted by the public and is convenient to take and carry.

The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention are intended to be included within the scope of the present invention.

Claims

1. A soluble snake gall chrysanthemum tea is characterized in that: the soluble snake gall chrysanthemum tea comprises snake gall, chrysanthemum, dried orange peel, frosted mulberry leaf and liquorice; the raw materials comprise the following components in parts by mass: 0.5-2 parts of snake gall, 10-30 parts of chrysanthemum, 30-50 parts of dried orange peel, 5-15 parts of frosted mulberry leaf and 5-15 parts of liquorice.

2. The soluble snake gall chrysanthemum tea as claimed in claim 1, wherein: the raw materials comprise, by mass, 1 part of snake gall, 20 parts of chrysanthemum, 40 parts of dried orange peel, 10 parts of frosted mulberry leaf and 10 parts of liquorice.

3. The soluble snake gall chrysanthemum tea as claimed in claim 1, wherein: the raw materials also comprise 0.05-0.1 part of menthol by mass.

4. The method for preparing the soluble snake gall chrysanthemum tea as claimed in any one of claims 1 to 3, which is characterized in that: the method comprises the following steps:

5. The method for making the soluble snake gall chrysanthemum tea as claimed in claim 4, wherein the method comprises the following steps: the temperature of the water bath in the first step is 50-80 ℃.

6. The method for making the soluble snake gall chrysanthemum tea as claimed in claim 4, wherein the method comprises the following steps: in the second step, the reflux decoction time after the first soaking is as follows: soaking for 20-40 minutes, and carrying out reflux decoction for 2-3 hours, wherein the second reflux decoction time is as follows: decocting under reflux for 1-2 hr.

7. The method for making the soluble snake gall chrysanthemum tea as claimed in claim 4, wherein the method comprises the following steps: the mass ratio of the sucrose dextrin in the third step is 1-5: 1-7.

8. The method for making the soluble snake gall chrysanthemum tea as claimed in claim 4, wherein the method comprises the following steps: 1g of snake gall in the first step, 20g of chrysanthemum, 40g of dried orange peel, 10g of frosted mulberry leaf and 10g of liquorice in the second step, and 0.08g of menthol in the third step.