CN113774104A - Method for preparing peptone and bio-organic fertilizer by using chondroitin sulfate wastewater - Google Patents
Method for preparing peptone and bio-organic fertilizer by using chondroitin sulfate wastewater Download PDFInfo
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- CN113774104A CN113774104A CN202111097568.6A CN202111097568A CN113774104A CN 113774104 A CN113774104 A CN 113774104A CN 202111097568 A CN202111097568 A CN 202111097568A CN 113774104 A CN113774104 A CN 113774104A
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- peptone
- chondroitin sulfate
- wastewater
- organic fertilizer
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/44—Treatment of water, waste water, or sewage by dialysis, osmosis or reverse osmosis
- C02F1/442—Treatment of water, waste water, or sewage by dialysis, osmosis or reverse osmosis by nanofiltration
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/40—Treatment of liquids or slurries
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/50—Treatments combining two or more different biological or biochemical treatments, e.g. anaerobic and aerobic treatment or vermicomposting and aerobic treatment
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A20/00—Water conservation; Efficient water supply; Efficient water use
- Y02A20/124—Water desalination
- Y02A20/131—Reverse-osmosis
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The invention discloses a method for preparing peptone and a bio-organic fertilizer by using chondroitin sulfate wastewater, which comprises the following steps: filtering the chondroitin sulfate wastewater by a sodium filter membrane; putting the desalted wastewater into an enzymolysis tank, adjusting the pH value by using an alkaline regulator, adding compound protease, performing enzymolysis under the action of an ultrasonic generator, and adding an acidic regulator to adjust the pH value; heating the enzymolysis tank to inactivate enzyme, and then cooling; filtering the solution with the floccules to obtain a clear solution, concentrating the clear solution, and performing spray drying to obtain a peptone product; adding fermentation liquor into the solution left after the preparation of the peptone, adding the fermentation liquor into a heat-preservation tank for sealed fermentation culture, and obtaining the organic lactic acid bacteria agent. The method can extract animal protein resources in the chondroitin sulfate wastewater to form peptone and organic fertilizer, so as to avoid water resource pollution and waste, reduce the wastewater treatment pressure in the production process of the chondroitin sulfate and reduce the production cost of the chondroitin sulfate.
Description
Technical Field
The invention relates to the technical field of sewage treatment, in particular to a method for preparing peptone and a biological organic fertilizer by using chondroitin sulfate wastewater.
Background
Chondroitin sulfate wastewater is a large amount of high-concentration organic wastewater generated in the production process of some biochemical product companies.
The chondroitin sulfate wastewater is special wastewater, contains a large amount of animal protein, and if the chondroitin sulfate wastewater is directly discharged without treatment, eutrophication of a water body is caused, and serious pollution is caused to water resources. If the treatment is carried out by adopting a sewage treatment mode, the production cost of the chondroitin sulfate is increased, and a large amount of animal protein in the water is wasted.
Disclosure of Invention
The invention aims to provide a method for preparing peptone and a bio-organic fertilizer by using chondroitin sulfate wastewater.
In order to achieve the purpose, the technical scheme adopted by the invention is as follows: a method for preparing peptone and a bio-organic fertilizer by using chondroitin sulfate wastewater comprises the following steps:
(a) desalting treatment: filtering the chondroitin sulfate wastewater through a sodium filter membrane to remove sodium chloride in the wastewater;
(b) and (3) pH adjustment: putting the desalted wastewater into an enzymolysis tank, adjusting the pH value to 6.5-7.5 by using an alkaline regulator, adding 1-1.2% of compound protease, carrying out enzymolysis for 2-3.5 hours under the action of an ultrasonic generator, and adding an acidic regulator to adjust the pH value to 5.1-5.4;
(c) enzyme deactivation treatment: heating the enzymolysis tank to 90-95 ℃ for inactivating enzyme for 15 minutes, then cooling to 55-65 ℃, stopping cooling when floccules are generated, and preserving heat for 30 minutes;
(d) preparing peptone: filtering the solution with the floccules to obtain a clear solution, and concentrating the clear solution, and then performing spray drying to obtain a peptone product;
(e) preparing an organic fertilizer: adding fermentation liquor into the residual concentrated solution of the peptone, adding one of the fermentation liquor into a heat-insulating tank for sealed fermentation culture to obtain an organic lactic acid bacteria agent, and performing vacuum concentration on the organic lactic acid bacteria agent by using a vacuum concentration evaporator to obtain an organic fertilizer with the solid content of 45-55%.
Preferably, the fermentation liquid in the step (e) is a mixed liquid obtained by fermenting molasses, dipotassium hydrogen phosphate, yeast extract and lactic acid bacteria for 8-14h, and the contents of the molasses, the dipotassium hydrogen phosphate, the yeast extract and the lactic acid bacteria in the remaining solution for preparing peptone are 1.8-3%, 0.1%, 0.3-0.8% and 2-5%.
Preferably, the specific steps of step (d) are as follows: and (c) filtering the solution in the step (c) by using a plate frame to obtain a clear solution, filtering the clear solution by using molecular membrane filtration equipment, concentrating the clear solution, and performing spray drying to obtain a peptone product.
Preferably, the molecular membrane is a molecular membrane with the molecular weight of 2000-3500 Da.
Preferably, in the step (b), the alkaline regulator is NAOH, and the acidic regulator is hydrochloric acid.
Peptone prepared by any one of the methods.
An organic fertilizer prepared by any one of the above methods.
Compared with the prior art, the invention has the advantages that:
the method can extract animal protein resources in the chondroitin sulfate wastewater to form peptone and organic fertilizer, so as to avoid water resource pollution and waste, reduce the wastewater treatment pressure in the production process of the chondroitin sulfate and reduce the production cost of the chondroitin sulfate.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a graph of the effect of different pH values on organic capture rate;
FIG. 2 is a graph showing the change in the diameter of the zone of inhibition during the culture in the medium (I), (II), and (III);
FIG. 3 is an appearance diagram of peptone prepared from chondroitin of the present invention;
FIG. 4 is an appearance of general peptone.
Detailed Description
The preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings so that the advantages and features of the present invention can be more easily understood by those skilled in the art, and the scope of the present invention will be more clearly and clearly defined.
The invention provides a method for preparing peptone and a bio-organic fertilizer by using chondroitin sulfate wastewater, which comprises the following steps:
example one
(a) Desalting treatment: sodium chloride is added into the residual wastewater after the extraction of the chondroitin sulfate due to process reasons, and in order to prevent the sodium chloride from salting out proteins without affecting the quality of the peptone, the sodium chloride in the wastewater is removed by filtering the chondroitin sulfate wastewater through a sodium filter membrane;
(b) and (3) pH adjustment: putting the desalted wastewater into an enzymolysis tank, adjusting the pH value to 7 by using an alkaline regulator, extracting by using an alkaline solution to degrade and denature protein, adding 1% of compound protease, performing enzymolysis for 2 hours under the action of an ultrasonic generator, adding an acidic regulator to adjust the pH value to 5.1, and forming a precipitate by using the protein solution with the lowest protein solubility at an isoelectric point, wherein the alkaline regulator is NAOH and the acidic regulator is hydrochloric acid;
(c) enzyme deactivation treatment: heating the enzymolysis tank to 90 ℃ to inactivate enzyme for 15 minutes, eliminating protease, preventing enzymolysis of protein, then cooling to 55 ℃, stopping cooling when floccules are generated, and keeping the temperature for 30 minutes;
(d) preparing peptone: filtering the solution in the step (c) by using a plate-and-frame filter to obtain a clear solution, filtering by using molecular membrane filtration equipment, wherein a molecular membrane with the molecular weight of 2000Da is adopted as the molecular membrane, the rest is a concentrated solution, and concentrating and then carrying out spray drying on the clear solution to obtain a peptone product;
(e) preparing an organic fertilizer: adding fermentation liquor into the residual concentrated solution of the peptone, adding one of the fermentation liquor into a heat-insulation tank for sealed fermentation culture to obtain an organic lactic acid bacteria agent, and performing vacuum concentration on the organic lactic acid bacteria agent by using a vacuum concentration evaporator to obtain an organic fertilizer with the solid content of 45-55%; wherein the fermentation liquid is a mixed liquid composed of molasses, dipotassium hydrogen phosphate, yeast extract and lactobacillus, and the contents of molasses, dipotassium hydrogen phosphate, yeast extract and lactobacillus in the remaining solution for preparing peptone are 1.8%, 0.1%, 0.3% and 2%.
The peptone produced by the method belongs to spectrum peptone and is suitable for culturing microorganisms.
Example two
(a) Desalting treatment: filtering the chondroitin sulfate wastewater through a sodium filter membrane to remove sodium chloride in the wastewater;
(b) and (3) pH adjustment: putting the desalted wastewater into an enzymolysis tank, adjusting the pH value to 7.3 by using an alkaline regulator, adding 1.1% of compound protease, carrying out enzymolysis for 2-3.5 hours under the action of an ultrasonic generator, and then adding an acidic regulator to adjust the pH value to 5.2, wherein the alkaline regulator is NAOH, and the acidic regulator is hydrochloric acid;
(c) enzyme deactivation treatment: heating the enzymolysis tank to 93 ℃ for enzyme deactivation, then cooling to 60 ℃, stopping cooling when floccules are generated, and keeping the temperature for 30 minutes;
(d) preparing peptone: filtering the solution obtained in the step (c) by using a plate-and-frame filter to obtain a clear solution, filtering by using molecular membrane filtration equipment, wherein the molecular membrane adopts a molecular membrane with the molecular weight of 2500Da, the rest after filtration is pus, and concentrating and spray-drying the clear solution to obtain a peptone product;
(e) preparing an organic fertilizer: adding fermentation liquor into the residual concentrated solution of the peptone, adding one of the fermentation liquor into a heat-insulation tank for sealed fermentation culture to obtain an organic lactic acid bacteria agent, and performing vacuum concentration on the organic lactic acid bacteria agent by using a vacuum concentration evaporator to obtain an organic fertilizer with the solid content of 45-55%; wherein the fermentation liquid is a mixed liquid composed of molasses, dipotassium hydrogen phosphate, yeast extract and lactobacillus, and the contents of molasses, dipotassium hydrogen phosphate, yeast extract and lactobacillus in the remaining solution for preparing peptone are 2.2%, 0.1%, 0.5% and 3%.
EXAMPLE III
(a) Desalting treatment: filtering the chondroitin sulfate wastewater through a sodium filter membrane to remove sodium chloride in the wastewater;
(b) and (3) pH adjustment: putting the desalted wastewater into an enzymolysis tank, adjusting the pH value to 7.5 by using an alkaline regulator, adding 1.2% of compound protease, carrying out enzymolysis for 3.5 hours under the action of an ultrasonic generator, and then adding an acidic regulator to adjust the pH value to 5.4, wherein the alkaline regulator is NAOH, and the acidic regulator is hydrochloric acid;
(c) enzyme deactivation treatment: heating the enzymolysis tank to 95 ℃ for enzyme deactivation, then cooling to 65 ℃, stopping cooling when floccules are generated, and preserving heat for 30 minutes;
(d) preparing peptone: filtering the solution in the step (c) by using a plate frame to obtain a clear solution, filtering by using molecular membrane filtering equipment, wherein the molecular membrane adopts a molecular membrane with the molecular weight of 3500Da, the rest is a concentrated solution, and concentrating and spray-drying the clear solution to obtain a peptone product;
(e) preparing an organic fertilizer: adding fermentation liquor into the residual concentrated solution of the peptone, adding one of the fermentation liquor into a heat-insulation tank for sealed fermentation culture to obtain an organic lactic acid bacteria agent, and performing vacuum concentration on the organic lactic acid bacteria agent by using a vacuum concentration evaporator to obtain an organic fertilizer with the solid content of 45-55%; wherein the fermentation liquor is a mixed liquor consisting of molasses, dipotassium hydrogen phosphate, yeast extract and lactic acid bacteria, and the contents of the molasses, the dipotassium hydrogen phosphate, the yeast extract and the lactic acid bacteria in the remaining solution for preparing the peptone are 3%, 0.1%, 0.8% and 5%;
under a proper pH value, AL3+ in the wastewater is hydrolyzed to obtain AL (OH) 3, AL (OH) 4-, AL (OH) 2+ and the like, the device can adsorb organic matters and solid particles, the polynuclear polyhydroxy compound forms a reticular compound which can trap organic compounds and suspended solids and precipitate in large granular flocs, as shown in figure 1, the influence of different pH values on the organic matter trapping rate is obtained through experiments aiming at a plurality of groups of solutions added with acid regulators with different pH values.
As can be seen from FIG. 1, the pH value is between 5.1 and 5.4, the capture rate of organic substances is higher than that of the pH value in other ranges, the solubility of the protein solution is lowest, the amount of formed floc is more than that of the floc in other pH values, and particularly, the capture rate is highest when the pH value is 5.4.
The fermentation liquor is a mixed liquor consisting of molasses, dipotassium hydrogen phosphate, yeast extract and lactic acid bacteria, so that the fermentation yield of microorganisms can be improved;
three 10g of the remaining solution of the same peptone preparation were added to the medium of different mixtures as follows: 10g of beef extract, 5g of trihydrate and sodium acetate, 2g of lactobacillus, 1000ml of distilled water and 20g of agar; (II) 5g of molasses, 5g of dipotassium phosphate, 10g of yeast extract, 2g of lactic acid bacteria, 1000ml of distilled water and 20g of agar; (III) 5g of sodium chloride, 5g of glucose, 10g of beef extract, 2g of lactic acid bacteria, 1000ml of distilled water and 20g of agar;
inoculating the strain on a three-part culture medium flat plate, activating for 10h at 39 ℃, then carrying out low-temperature culture for 48h at 6-15 ℃, respectively taking fermentation liquor in the three-part culture medium every 4h during culture, centrifuging for 10min, and then carrying out enzyme activity determination, wherein the determination result is shown in figure 2, the curve of the highest position is a (second) culture medium, the concentration of the strain is rapidly increased along with the increase of the culture time, and the diameter of a bacteriostatic circle of the strain is continuously increased along with the continuous reproduction of the strain, which indicates that the activity of the strain is increased, and the concentration of the strain is not obviously increased and the bacteriostatic circle is not increased any more at 40-48h and is in a stable period, and the strain reproduction speed of the (second) culture medium is faster and more stable than that of the (first) culture medium and the (third) culture medium.
The table below shows the comparison of peptone prepared from the residual concentrated solution after extracting peptone from chondroitin sulfate wastewater with standard and common peptone;
Residual concentrated solution after extracting peptone from chondroitin sulfate wastewater is adopted, and the purchase cost is basically negligible;
and 20 cubic fermentation liquor is needed for producing one ton of the organic lactic acid bacteria, 200 kilograms of peptone are needed according to 1 percent of the proportion of the nitrogen source needed in the formula of the lactic acid bacteria culture medium, the cost is saved by 200 kilograms per 30 yuan/kilogram for producing one ton of the organic lactic acid bacteria according to the market price of 30 yuan/kilogram of the peptone.
Although the embodiments of the present invention have been described with reference to the accompanying drawings, various changes or modifications may be made by the patentees within the scope of the appended claims, and within the scope of the invention, as long as they do not exceed the scope of the invention described in the claims.
Claims (7)
1. A method for preparing peptone and a bio-organic fertilizer by using chondroitin sulfate wastewater is characterized by comprising the following steps:
(a) desalting treatment: filtering the chondroitin sulfate wastewater through a sodium filter membrane to remove sodium chloride in the wastewater;
(b) and (3) pH adjustment: putting the desalted wastewater into an enzymolysis tank, adjusting the pH value to 6.5-7.5 by using an alkaline regulator, adding 1-1.2% of compound protease, carrying out enzymolysis for 2-3.5 hours under the action of an ultrasonic generator, and adding an acidic regulator to adjust the pH value to 5.1-5.4;
(c) enzyme deactivation treatment: heating the enzymolysis tank to 90-95 ℃ for inactivating enzyme for 15 minutes, then cooling to 55-65 ℃, stopping cooling when floccules are generated, and preserving heat for 30 minutes;
(d) preparing peptone: filtering the solution with the floccules to obtain a clear solution, and concentrating the clear solution, and then performing spray drying to obtain a peptone product;
(e) preparing an organic fertilizer: adding fermentation liquor into the residual concentrated solution of the peptone, adding one of the fermentation liquor into a heat-insulating tank for sealed fermentation culture to obtain an organic lactic acid bacteria agent, and performing vacuum concentration on the organic lactic acid bacteria agent by using a vacuum concentration evaporator to obtain an organic fertilizer with the solid content of 45-55%.
2. The method for preparing peptone and bio-organic fertilizer from chondroitin sulfate wastewater as claimed in claim 1, wherein: and (e) the fermentation liquor in the step (e) is a mixed liquor of molasses, dipotassium hydrogen phosphate, yeast extract and lactic acid bacteria after anaerobic fermentation for 8-14 hours, and the content of the molasses, the dipotassium hydrogen phosphate, the yeast extract and the lactic acid bacteria in the residual solution for preparing the peptone is 1.8-3%, 0.1%, 0.3-0.8% and 2-5%.
3. The method for preparing peptone and bio-organic fertilizer from chondroitin sulfate wastewater as claimed in claim 1, wherein the specific steps of step (d) are as follows: and (c) filtering the solution in the step (c) by using a plate frame to obtain a clear solution, filtering the clear solution by using molecular membrane filtration equipment, concentrating the clear solution, and performing spray drying to obtain a peptone product.
4. The method for preparing peptone and bio-organic fertilizer from chondroitin sulfate wastewater as claimed in claim 3, wherein: the molecular membrane adopts a molecular membrane with the molecular weight of 2000-3500 Da.
5. The method for preparing peptone and bio-organic fertilizer from chondroitin sulfate wastewater as claimed in claim 1, wherein: in the step (b), the alkaline regulator is NaOH, and the acidic regulator is hydrochloric acid.
6. A peptone, characterized in that: the product is prepared by the method of any one of claims 1 to 5.
7. An organic fertilizer is characterized in that: the product is prepared by the method of any one of claims 1 to 5.
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| CN202111097568.6A CN113774104A (en) | 2021-09-18 | 2021-09-18 | Method for preparing peptone and bio-organic fertilizer by using chondroitin sulfate wastewater |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114805635A (en) * | 2022-05-10 | 2022-07-29 | 滨海宇美科技有限公司 | Desalination protein powder and sodium chondroitin sulfate co-production process |
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2021
- 2021-09-18 CN CN202111097568.6A patent/CN113774104A/en not_active Withdrawn
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114805635A (en) * | 2022-05-10 | 2022-07-29 | 滨海宇美科技有限公司 | Desalination protein powder and sodium chondroitin sulfate co-production process |
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Address after: 256400 No. 97, Jianlong Road, Guoli Town, Huantai County, Zibo City, Shandong Province Applicant after: Zibo Jinyuan Biotechnology Co.,Ltd. Address before: 256400 No. 97, Jianlong Road, Guoli Town, Huantai County, Zibo City, Shandong Province Applicant before: Zibo Jinyuan Biotechnology Co.,Ltd. |
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Application publication date: 20211210 |