CN113768143A - Eucommia ulmoides peptide total nutrient powder and preparation method thereof - Google Patents
Eucommia ulmoides peptide total nutrient powder and preparation method thereof Download PDFInfo
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- CN113768143A CN113768143A CN202110577780.6A CN202110577780A CN113768143A CN 113768143 A CN113768143 A CN 113768143A CN 202110577780 A CN202110577780 A CN 202110577780A CN 113768143 A CN113768143 A CN 113768143A
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- 201000006370 kidney failure Diseases 0.000 description 1
- 229920005610 lignin Polymers 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 210000005229 liver cell Anatomy 0.000 description 1
- 235000004213 low-fat Nutrition 0.000 description 1
- 210000003141 lower extremity Anatomy 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000007102 metabolic function Effects 0.000 description 1
- 230000027939 micturition Effects 0.000 description 1
- 239000002366 mineral element Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 150000002772 monosaccharides Chemical class 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- -1 natural pH Substances 0.000 description 1
- 229930014626 natural product Natural products 0.000 description 1
- 231100000957 no side effect Toxicity 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 229920001542 oligosaccharide Polymers 0.000 description 1
- 150000002482 oligosaccharides Chemical class 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000011164 ossification Effects 0.000 description 1
- ZQTHOIGMSJMBLM-BUJSFMDZSA-N pangamic acid Chemical compound CN(C)CC(=O)OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C(O)=O ZQTHOIGMSJMBLM-BUJSFMDZSA-N 0.000 description 1
- 108700024047 pangamic acid Proteins 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 230000008855 peristalsis Effects 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 229930015704 phenylpropanoid Natural products 0.000 description 1
- 150000002995 phenylpropanoid derivatives Chemical class 0.000 description 1
- OHOPKHNWLCMLSW-UHFFFAOYSA-N pinoresinol Natural products C1=C(O)C(OC)=CC(C2C3C(C(OC3)C=3C=C(CO)C(O)=CC=3)CO2)=C1 OHOPKHNWLCMLSW-UHFFFAOYSA-N 0.000 description 1
- 235000007221 pinoresinol Nutrition 0.000 description 1
- 210000003635 pituitary gland Anatomy 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000010791 quenching Methods 0.000 description 1
- 230000000171 quenching effect Effects 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 230000003248 secreting effect Effects 0.000 description 1
- 210000004927 skin cell Anatomy 0.000 description 1
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Inorganic materials [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 description 1
- 239000003270 steroid hormone Substances 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 150000005846 sugar alcohols Chemical class 0.000 description 1
- 235000021092 sugar substitutes Nutrition 0.000 description 1
- 235000021147 sweet food Nutrition 0.000 description 1
- 239000003765 sweetening agent Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 230000035922 thirst Effects 0.000 description 1
- 230000002936 tranquilizing effect Effects 0.000 description 1
- 210000001835 viscera Anatomy 0.000 description 1
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 description 1
- BURBOJZOZGMMQF-UHFFFAOYSA-N xanthoxylol Natural products C1=C(O)C(OC)=CC=C1C1C(COC2C=3C=C4OCOC4=CC=3)C2CO1 BURBOJZOZGMMQF-UHFFFAOYSA-N 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The invention discloses eucommia ulmoides peptide total nutrient powder which comprises the following raw materials in parts by weight: 1000-1200 parts of eucommia leaf, 10-12 parts of bran and 50-72 parts of mixed bacterial liquid; the preparation method comprises the following steps: (1) weighing the raw materials in parts by weight according to the eucommia ulmoides peptide total nutrient powder for later use; (2) pulverizing folium Eucommiae, sieving, and mixing with testa Tritici under stirring to obtain mixture A; (3) adding water into the mixture A, then adding the mixed bacteria liquid, and stirring uniformly at room temperature to obtain a mixture B; (4) fermenting the mixture B at 25-35 deg.C for 5-6 days, adding water for enzymolysis; continuously adding water, heating to 50-60 ℃, performing reflux extraction, and filtering to obtain filtrate I and filter residue I; (5) adding water into the filter residue I, heating to 50-60 ℃, performing reflux extraction, and filtering to obtain a filtrate II and a filter residue II; mixing the filtrate I and the filtrate II to obtain a filtrate III; (6) and adding a chitosan solution into the filtrate III, stirring, clarifying, standing, concentrating the clear liquid, drying and crushing to obtain the eucommia ulmoides peptide total nutrient powder.
Description
Technical Field
The invention relates to the technical field of health care products, in particular to eucommia ulmoides peptide total nutrient powder and a preparation method thereof.
Background
The functional beverage is a soft beverage which is added with certain functional factors and has the health-care functions of regulating the body function, enhancing the immunity and the like while quenching thirst. The functional beverage is a new growth point of food industry at home and abroad at present. In view of the attention of consumers to food safety in recent years, the extraction of functional food additives and ingredients from natural products, particularly from commonly consumed plants, has become a hot spot for development at home and abroad. In western countries, due to unreasonable dietary structure, excessive intake of fat and protein, and common overweight people and patients with hyperlipidemia and hypertension, development of low-calorie and low-fat food and additives has been emphasized in europe and the united states. Sugar alcohol sugar substitutes are used for producing sugar-free sweet foods, fat-replacing foods using products such as inulin and the like all take the leading position internationally. In Asian countries, there are traditions of homology of medicine and food, and the development of health-care and functional food and beverage is emphasized. The obvious efficacy of functional food additives, in particular of some natural extracts, is internationally recognized. Therefore, a large number of health functional foods enter the international market in the form of food supplements.
At present, the eucommia ulmoides and eucommia male flowers are used as raw materials for preparing the beverage, the lignin, iridoid, phenylpropanoid active substances (such as geniposide acid, chlorogenic acid and aucubin), pinoresinol diglucoside, flavone, alkaloid, amino acid, polysaccharide and mineral elements contained in the eucommia ulmoides have unique pharmacological and health-care effects, the eucommia ulmoides male flowers collect all essences of the eucommia ulmoides, have the effects of reducing blood pressure and blood fat, have no side effect for a long time, can promote coronary artery blood circulation, treat cardiovascular and cerebrovascular diseases and are considered to be natural blood pressure reducing medicines with highest quality in the world. Can accelerate the metabolism of collagen of human body, prevent or delay skin wrinkling and aging, increase skin luster, improve hair texture, reduce alopecia, delay aging and prevent senile dementia, and is considered to be one of four anti-aging medicaments in the world. The eucommia male flower also has the health care effects of calming, improving sleep, losing weight, regulating estrogen, maintaining beauty and keeping young. These functional beverages only exhibit the efficacy of the eucommia male flowers.
Therefore, the problem to be solved by the technical personnel in the field is how to provide a preparation method of the eucommia ulmoides peptide total nutrient powder with high protein content, high nutritional value and high plant utilization rate.
Disclosure of Invention
In view of the above, the invention provides a preparation method and application of eucommia peptide total nutrient powder, which utilizes the original nutrient components in eucommia leaves to produce more nutrient components such as protein through mixed bacteria fermentation, thereby greatly improving the protein content in the eucommia leaves; and the active ingredients of the extract can be exerted, thereby improving the utilization rate of plants; but also improves the nutritive value.
In order to achieve the purpose, the invention adopts the following technical scheme: the eucommia ulmoides peptide total nutrient powder is characterized by comprising the following raw materials in parts by weight:
1000-1200 parts of eucommia leaf, 10-12 parts of bran and 50-72 parts of mixed bacterial liquid.
The invention has the following beneficial effects: according to the invention, the mixed bacterial liquid is compounded with the folium cortex eucommiae, so that the protein content in the folium cortex eucommiae can be increased, and the nutritional value of the folium cortex eucommiae can be increased; moreover, the eucommia peptide total nutrient powder has the effects of reducing blood pressure and blood fat, protecting liver, tonifying kidney and improving sexual function, can enhance the immunity of organisms, relax bowels, promote urination, soothe nerves, nourish sleep, maintain beauty, keep young and improve the health care effect of obesity, and has complete functions, large effect and quick response.
The eucommia ulmoides leaves have the following functions:
the aucubin in the eucommia ulmoides leaves has obvious liver protection activity, can promote the regeneration of liver cells, can obviously inhibit the replication of hepatitis B virus DNA, and can promote the accumulation of glycogen, increase the blood sugar content and protect the liver. It also has effects in stimulating pituitary gland and adrenal cortex system, continuously enhancing adrenal cortex function (secreting steroid hormone), and improving sexual function.
The folium Eucommiae contains many active ingredients such as pinoresinol diglycoside, folium Persicae, chlorogenic acid, and polysaccharide, all have effects of lowering blood pressure and reducing blood lipid.
The eucommia ulmoides leaves for reducing blood sugar contain a plurality of alpha-glucosidase inhibiting components, and the alpha-glucosidase inhibiting components can block or delay the generation of glucose and the absorption of intestinal tracts, so that proper blood sugar value in vivo is maintained, and the eucommia ulmoides leaves have a good blood sugar reducing effect. The general chemical drugs have injuries to the liver and kidney of a human body while reducing blood sugar, but eucommia is different, and the eucommia has been researched by forever in China to have the function of tonifying the liver and the kidney, so that the eucommia has no injury to the liver and the kidney while reducing blood sugar, and has a remarkable maintenance function, which is a great characteristic of reducing blood sugar by the eucommia. The most common complications of diabetes are cardiovascular and cerebrovascular diseases, renal failure, gangrene of eyes, nerves and lower limbs, etc., and eucommia bark has the functions of diminishing inflammation, preventing mild and gradual change and preventing and repairing early injury of organs. The eucommia bark can obviously prevent diabetic complications while reducing the blood sugar, which is another characteristic of the eucommia bark in reducing the blood sugar.
The eucommia ulmoides leaves with the function of enhancing immunity are rich in polysaccharide active substances, can enhance the immunity of organisms, and has the function of bidirectionally regulating the cellular immunity and keeping the immune function of a human body in a good state all the time. Simultaneously, the medicine is involved in regulating the cardiovascular system and is very beneficial to maintaining the health of human bodies.
The aucubin in folium Eucommiae has diuretic, constipation relieving, and intestinal peristalsis promoting effects.
The natural active ingredients of the eucommia bark leaves for soothing nerves and nourishing sleep have the effects of soothing nerves, tranquilizing and relieving pain, and can obviously improve the sleep after being taken for a long time. The eucommia ulmoides leaves contain collagen essential to human bodies, have the effect of promoting muscle development and building, have obvious anti-fatigue effect on lignans serving as active ingredients, and have obvious effect on people who lack exercise capacity for long-term indoor work.
The aging of the skin for maintaining beauty and keeping young is mainly caused by the loss of elasticity of collagen among dermal cells under the skin, and natural active ingredients contained in the eucommia leaves can delay the aging of the collagen, accelerate the metabolism of the collagen and improve the synthesis capacity of the collagen, thereby preventing or delaying the wrinkling and aging of the skin and increasing the skin luster. Meanwhile, long-term administration of eucommia ulmoides can promote blood circulation and metabolic function, promote activation of brain cells and prevent senile dementia.
The eucommia bark leaves for improving obesity can obviously reduce the content of neutral fat under the skin and around the internal organs of a human body, and has the effects of preventing exercise, changing diet life, preventing obesity and losing weight. The eucommia ulmoides can promote the metabolism of collagen having the function of connecting cells and cells, accelerate the synthesis of other proteins, consume energy in vivo, and naturally reduce neutral fat accumulated in the body.
Lignans, iridoids and flavonoids in the anti-osteoporosis eucommia leaves can be the material basis of eucommia which can promote bone formation and inhibit bone absorption so as to play the anti-osteoporosis role.
Preferably, the mixed bacterial liquid is prepared by inoculating aspergillus niger, trichoderma and yeast into a liquid culture medium according to the mass ratio of (1-2) to (2-4) to (1-2), and stirring and culturing for 3-4 days at 25-35 ℃; the concentration of Aspergillus niger in the mixed bacterial liquid is 5-10%, the concentration of Trichoderma is 5-10% and the concentration of yeast is 5-10%.
Preferably, Aspergillus niger is cultured: aspergillus niger slant culture medium: KH (Perkin Elmer)2PO43.0g，MgSO4·7H2O1.5 g, vitamin B15 mug/mL, glucose 20g, agar 15g, potato extract 1000mL, pH6.0.
Culturing trichoderma: trichoderma slant culture medium: KH (Perkin Elmer)2PO41g，NaNO33g，KCl 0.5g，MgSO4·7H2O 0.5g，FeSO4·7H20.01g of O, 30g of cane sugar, 20g of agar, natural pH and 1000mL of distilled water.
Culturing yeast: and (3) yeast culture medium: 1% yeast extract, 2% peptone, 2% glucose, 2% agar, natural pH, distilled water 100 mL.
Preferably, the liquid culture medium is prepared from eucommia ulmoides leaf powder, sucrose or glucose and potato extract in a mass-volume ratio of (5-15) g: (15-25) g: 1000mL of the mixture was mixed.
The invention also provides a preparation method of the eucommia ulmoides peptide total nutrient powder, which comprises the following steps:
(1) weighing the raw materials in parts by weight according to the eucommia ulmoides peptide total nutrient powder for later use;
(2) crushing and sieving eucommia leaves, mixing the eucommia leaves with bran, and uniformly stirring the mixture and the bran under the condition of 150-;
(3) adding water into the mixture A, then adding the mixed bacteria liquid, and stirring uniformly at room temperature to obtain a mixture B;
(4) fermenting the mixture B at 25-35 deg.C for 5-6 days, adding water for enzymolysis; continuously adding water, heating to 50-60 ℃, performing reflux extraction, and filtering to obtain filtrate I and filter residue I;
(5) adding water into the filter residue I, heating to 50-60 ℃, performing reflux extraction, and filtering to obtain a filtrate II and a filter residue II; mixing the filtrate I and the filtrate II to obtain a filtrate III;
(6) and adding a chitosan solution into the filtrate III, stirring, clarifying, standing, concentrating the clear liquid, drying and crushing to obtain the eucommia ulmoides peptide total nutrient powder.
The invention has the following beneficial effects: the preparation method provided by the invention is complete in continuity, easy to operate and more suitable for large-scale industrial production.
Preferably, the mass ratio of the water to the eucommia ulmoides leaves in the mixture A is (50-60): 100, wherein the mass ratio of the eucommia ulmoides leaves in the mixture A to the mixed bacterial liquid is 100: (5-6);
the stirring speed is 80-120r/min, and the stirring time is 8-12 min.
Adopt above-mentioned technical scheme's beneficial effect: the above scheme is defined in (50-60): the proportion of 100 not only retains the moisture required by fermentation, but also retains the good air permeability required by fermentation, so that the fermentation can be well carried out under the conditions.
Preferably, in the step (4), the mass ratio of the water for adding water for enzymolysis to the eucommia ulmoides leaves in the mixture A is 4-6:1, the temperature for adding water for enzymolysis is 35-45 ℃, and the time is 4-6 h;
the mass ratio of the water added after enzymolysis to the eucommia ulmoides leaves in the mixture A is 2-4: 1; the reflux extraction time is 2-4 h.
Preferably, in the step (5), the mass ratio of the water to the filter residue I is 5-7: 1; the reflux extraction time is 2-3 h.
Preferably, in the step (6), the concentration of the chitosan solution is 1%, and the volume ratio of the chitosan solution to the filtrate III is 1-3: 100; the standing time is more than 2 hours; the drying temperature is 65-75 ℃, and the drying time is 6-8 h.
According to the technical scheme, compared with the prior art, the eucommia ulmoides peptide total nutrient powder and the preparation method thereof disclosed by the invention have the following beneficial effects:
according to the invention, by using the specific mixed bacterial liquid and the fermentation process, the original nutrient components in the eucommia ulmoides leaves are utilized to produce more nutrient components such as protein through mixed bacterial fermentation, and the protein content in the eucommia ulmoides leaves is greatly improved.
According to the invention, a large amount of proteins which are contained in the eucommia ulmoides leaves and are useful for a human body but not beneficial to absorption are converted into small molecular protein peptides which are beneficial to the absorption of the human body by using protease which is generated by fermenting and culturing the eucommia ulmoides leaves by using mixed bacteria, and the small molecular peptides have good solubility, emulsibility and biological activity (physiological activity, transportation physiological activity, carrier physiological activity, dynamic physiological activity, transmitter physiological activity and the like), improve the functional characteristics of the proteins, enhance the effects of all functional elements (aucubin, pinoresinol diglucoside, chlorogenic acid, polysaccharide, collagen and the like) of the eucommia ulmoides leaves, and are widely applied to common foods and functional foods; thereby solving the problem of low utilization rate of the effective components of the plants.
According to the invention, the amylase generated by fermentation culture of the eucommia leaves by using the mixed bacteria is used for hydrolyzing the macromolecular starch in the eucommia leaves, so that the starch which seriously influences the solubility of the product in the extracting solution is removed, and the starch is converted into monosaccharide which is convenient to absorb and utilize and soluble oligosaccharide with a health care effect; thereby solving the problems that the product solubility is not good easily due to low nutrition value, and the beta-glycan in the eucommia leaves has large molecular weight and is difficult to be absorbed by human body because of the compound chain structure.
According to the invention, cellulase produced by fermentation culture of eucommia leaves by using mixed bacteria is used for hydrolyzing eucommia leaf cell wall fibers, so that aucubin, pinoresinol diglucoside, chlorogenic acid, polysaccharide, collagen and the like in the eucommia leaves are leached out, and active ingredients of an extracting solution are exerted; thereby solving the problem of low extraction rate of the effective components of the plants.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The following mixed bacterial liquid is prepared by inoculating aspergillus niger, trichoderma and yeast into a liquid culture medium according to the mass ratio of 1:2:1, and culturing for 3-4 days under stirring.
Example 1
The eucommia peptide total nutrient powder comprises the following raw materials: 1000kg of eucommia leaves, 10kg of bran and 50kg of mixed bacterial liquid;
the preparation method of the eucommia ulmoides peptide total nutrient powder comprises the following steps:
crushing 1000kg of eucommia leaves, sieving the crushed eucommia leaves with a 10-mesh sieve, putting the crushed eucommia leaves and 10kg of bran into a material mixing pool, uniformly stirring the crushed eucommia leaves and the bran into the material mixing pool by a stirrer, adding water accounting for 50 percent of the mass of the eucommia leaves, uniformly stirring the mixture by the stirrer, adding 50kg of mixed bacterial liquid, uniformly stirring the mixture by the stirrer, conveying the mixture into a fermentation-enzymolysis-leaching integrated pool by a belt conveyor, compacting, covering, performing heat preservation fermentation culture at 25 ℃, properly stirring the mixture by a stirrer in the process, adding water accounting for 5 times of the mass of the eucommia leaves after 5 days, performing cyclic enzymolysis for 4 hours at 35 ℃, adding water accounting for 3 times of the mass of the eucommia leaves, performing cyclic heat reflux extraction for 3 hours at 50 ℃, and performing coarse filtration by a duplex filter to obtain filtrate I and filtrate I;
adding 6 times of tap water into the filter residue I, performing circulating heat reflux extraction at 50 ℃ for 2.0h, performing coarse filtration by using a duplex filter to obtain a filtrate II and a filter residue II, then combining the filtrate I and the filtrate II to obtain a filtrate III, cooling the filtrate III by using a pipeline cooler, then pumping the cooled filtrate III into a clarifying tank, adding a 1% chitosan solution with the filtrate amount of 2% (v/v) into the clarifying tank, stirring for 10min, performing clarification treatment, standing for more than 2h, performing sedimentation centrifugation by using a spiral continuous sedimentation centrifuge, filtering clear liquid by using a ceramic membrane filter, concentrating by using a reverse osmosis membrane concentrator, concentrating by using a low-temperature vacuum concentrator, drying by using a continuous low-temperature drying unit, and crushing to obtain the eucommia peptide total nutrient powder of 182.85 kg.
Example 2
The eucommia peptide total nutrient powder comprises the following raw materials: 1100kg of eucommia leaves, 11kg of bran and 60.5kg of mixed bacterial liquid;
the preparation method of the eucommia ulmoides peptide total nutrient powder comprises the following steps:
1100kg of eucommia leaves are crushed, sieved by a 10-mesh sieve, and put into a material mixing pool together with 11kg of bran, the mixture is stirred uniformly by a stirrer, then water accounting for 55 percent of the mass of the eucommia leaves is added, the mixture is stirred uniformly by the stirrer, 60.5kg of mixed bacterial liquid is inoculated, the mixture is stirred uniformly by the stirrer, conveyed into a fermentation-enzymolysis-leaching integrated pool by a belt conveyor, compacted and covered, the fermentation culture is carried out at the temperature of 30 ℃, proper material stirring is carried out by a material stirring machine during the fermentation culture, 5.5 days later, water accounting for 5 times of the mass of the eucommia leaves is added, the enzymolysis is carried out for 4 hours at the temperature of 40 ℃, water accounting for 3 times of the mass of the eucommia leaves is added, the extraction is carried out for 3 hours at the temperature of circulating heat reflux, and a duplex filter is roughly filtered to obtain filtrate I and filter residue I;
adding 6 times of tap water into the filter residue I, performing circulating heat reflux extraction at 55 ℃ for 2.0h, performing coarse filtration by using a duplex filter to obtain a filtrate II and a filter residue II, then combining the filtrate I and the filtrate II to obtain a filtrate III, cooling the filtrate III by using a pipeline cooler, then pumping the cooled filtrate III into a clarifying tank, adding a 1% chitosan solution with the filtrate amount of 2% (v/v) into the clarifying tank, stirring for 10min, performing clarification treatment, standing for more than 2h, performing sedimentation centrifugation by using a spiral continuous sedimentation centrifuge, filtering clear liquid by using a ceramic membrane filter, concentrating by using a reverse osmosis membrane concentrator, concentrating by using a low-temperature vacuum concentrator, drying by using a continuous low-temperature drying unit, and crushing to obtain the eucommia peptide total nutrient powder, wherein the weight of the eucommia peptide total nutrient powder is about 208.36 kg.
Example 3
The eucommia peptide total nutrient powder comprises the following raw materials: 1200kg of eucommia ulmoides leaves, 12kg of bran and 72kg of mixed bacterial liquid;
the preparation method of the eucommia ulmoides peptide total nutrient powder comprises the following steps:
1200kg of eucommia leaves are crushed, sieved by a 10-mesh sieve, and put into a material mixing pool together with 12kg of bran, the mixture is stirred uniformly by a stirrer, then water accounting for 60 percent of the mass of the eucommia leaves is added, the mixture is stirred uniformly by the stirrer, 72kg of mixed bacterial liquid is added, the mixture is stirred uniformly by the stirrer, conveyed into a fermentation-enzymolysis-leaching integrated pool by a belt conveyor, compacted and covered, the fermentation culture is carried out at the temperature of 35 ℃, proper material stirring is carried out by a material stirring machine during the fermentation culture, after 6 days, water accounting for 5 times of the mass of the eucommia leaves is added, circular enzymolysis is carried out for 4 hours at the temperature of 45 ℃, water accounting for 3 times of the mass of the eucommia leaves is added, circular heat reflux extraction is carried out for 3 hours at the temperature of 60 ℃, and coarse filtration by a duplex filter is carried out to obtain filtrate I and filter residue I;
adding 6 times of tap water into the filter residue I, performing circulating heat reflux extraction at 60 ℃ for 2.0h, performing coarse filtration by using a duplex filter to obtain a filtrate II and a filter residue II, then combining the filtrate I and the filtrate II to obtain a filtrate III, cooling the filtrate III by using a pipeline cooler, then pumping the cooled filtrate III into a clarifying tank, adding a 1% chitosan solution with the filtrate amount of 2% (v/v) into the clarifying tank, stirring for 10min, performing clarification treatment, standing for more than 2h, performing sedimentation centrifugation by using a spiral continuous sedimentation centrifuge, filtering clear liquid by using a ceramic membrane filter, concentrating by using a reverse osmosis membrane concentrator, concentrating by using a low-temperature vacuum concentrator, drying by using a continuous low-temperature drying unit, and crushing to obtain the eucommia peptide total nutrient powder, wherein the weight of the eucommia peptide total nutrient powder is about 232.15 kg.
Test of oxidation resistance and blood sugar reducing capability of eucommia ulmoides peptide total nutrient powder
1. DPPH.determination of scavenging Capacity
The quercetin is used as a positive control, and the eucommia peptide total nutrient powder prepared in the embodiments 1-3 of the invention with different concentrations is used for preparing nutrient solution with a certain concentration and distilled water for detecting DPPH and scavenging capacity; the results of the experiment are shown in table 1 below:
TABLE 1 IC for DPPH-scavenging of eucommia ulmoides peptide total nutrient powder of different purity50Value of
The experimental results in table 1 above show that: the nutrient solution prepared from 100% eucommia peptide total nutrient powder has optimal DPPH and scavenging ability, IC50The value is 72.82, 69.94, 67.72 mug DM/mL, the IC is less than that of a nutrient solution prepared by 80% eucommia peptide total nutrient powder50The nutrient solution prepared by using 20% eucommia peptide total nutrient powder with the concentration of 76.26, 73.21 and 70.95 mu g DM/mL has relatively poor effect and IC50112.81, 108.35, 100.77 μ g DM/mL. The eucommia ulmoides peptide total nutrient powder has DPPH removing capability, and the effect is weakened along with the reduction of the purity of the eucommia ulmoides peptide total nutrient powder.
2. ABTS +. scavenging Capacity determination
Quercetin is used as a positive control, and eucommia peptide total nutrient powder prepared in the embodiments 1-3 of the invention with different concentrations is prepared into nutrient solution with a certain concentration and distilled water to detect ABTS +. scavenging capacity; the results of the experiment are shown in table 2 below:
TABLE 2 IC for eliminating ABTS +. by eucommia ulmoides peptide total nutrient powder with different purity50Value of
The experimental results in table 2 above show that: the nutrient solution prepared from 100% eucommia ulmoides peptide total nutrient powder has the best ABTS +. scavenging ability, IC50The value is 165.92, 159.31, 154.35 μ g DM/mL, and the IC is less than that of a nutrient solution prepared by 80% eucommia peptide total nutrient powder50The nutrient solution prepared from 20% eucommia peptide total nutrient powder with 170.06, 163.22 and 158.11 mug DM/mL has relatively poor effect and IC50242.86, 233.18, 225.89 μ g DM/mL. The eucommia ulmoides peptide total nutrient powder has ABTS +. scavenging capacity, and the effect is weakened along with the reduction of the purity of the eucommia ulmoides peptide total nutrient powder.
3. Measurement of alpha-glucosidase Activity inhibitory Activity
Quercetin is used as a positive control, and eucommia peptide total nutrient powder prepared by the embodiment of the invention with different concentrations is prepared into nutrient solution with certain concentration and distilled water to detect the activity inhibition capability of the alpha-glucosidase; the results of the experiment are shown in table 3 below:
TABLE 3 IC for inhibiting alpha-glucosidase activity of eucommia ulmoides peptide total nutrient powder with different purities50Value of
The experimental results in table 3 above show that: the nutrient solution prepared from 100% eucommia ulmoides peptide total nutrient powder has optimal alpha-glucosidase activity inhibition ability, IC50Values of 96.62, 92.72, 8981 μ g DM/mL, a nutritional liquid formulated with 80% eucommia ulmoides peptide total nutrient powder, IC50The nutrient solution prepared from 20% eucommia peptide total nutrient powder with 114.16, 109.56 and 101.87 mug DM/mL has relatively poor effect and IC50155.66, 149.49, 139.08 μ g DM/mL. The eucommia ulmoides peptide total nutrient powder has alpha-glucosidase activity inhibition capacity, and the effect is weakened along with the reduction of the purity of the eucommia ulmoides peptide total nutrient powder.
In conclusion, experiments show that the eucommia peptide total nutrient powder has stronger DPPH removing capability and ABTS +/removing capability, which shows that the eucommia peptide total nutrient powder has good oxidation resistance; the eucommia ulmoides peptide total nutrient powder has strong capacity of inhibiting the activity of alpha-glucosidase, and can block or delay the generation of glucose and the absorption of intestinal tracts, thereby maintaining proper blood sugar value in vivo and showing that the eucommia ulmoides peptide total nutrient powder has good blood sugar reducing effect.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (8)
1. The eucommia ulmoides peptide total nutrient powder is characterized by comprising the following raw materials in parts by weight:
1000-1200 parts of eucommia leaf, 10-12 parts of bran and 50-72 parts of mixed bacterial liquid.
2. The eucommia ulmoides peptide total nutrient powder as claimed in claim 1, wherein the mixed bacterial liquid is prepared by inoculating Aspergillus niger, Trichoderma asperellum and yeast in a mass ratio of (1-2) to (2-4) to (1-2) in a liquid culture medium, and culturing at 25-35 ℃ for 3-4 days under stirring.
3. The eucommia ulmoides peptide total nutrient powder as claimed in claim 2, wherein the mass-to-volume ratio of the liquid culture medium consisting of eucommia ulmoides leaf powder, sucrose or glucose and potato extract is (5-15) g: (15-25) g: 1000mL of the mixture was mixed.
4. A preparation method of eucommia ulmoides peptide total nutrient powder is characterized by comprising the following steps:
(1) weighing the raw materials in parts by weight according to the eucommia ulmoides peptide total nutrient powder of any one of claims 1 to 3 for later use;
(2) pulverizing folium Eucommiae, sieving, and mixing with testa Tritici under stirring to obtain mixture A;
(3) adding water into the mixture A, then adding the mixed bacteria liquid, and stirring uniformly at room temperature to obtain a mixture B;
(4) fermenting the mixture B at 25-35 deg.C for 5-6 days, adding water for enzymolysis; continuously adding water, heating to 50-60 ℃, performing reflux extraction, and filtering to obtain filtrate I and filter residue I;
(5) adding water into the filter residue I, heating to 50-60 ℃, performing reflux extraction, and filtering to obtain a filtrate II and a filter residue II; mixing the filtrate I and the filtrate II to obtain a filtrate III;
(6) and adding a chitosan solution into the filtrate III, stirring, clarifying, standing, concentrating the clear liquid, drying and crushing to obtain the eucommia ulmoides peptide total nutrient powder.
5. The preparation method of the eucommia ulmoides peptide total nutrient powder as claimed in claim 4, wherein in the step (3), the mass ratio of the water to the eucommia ulmoides leaves in the mixture A is (50-60): 100, wherein the mass ratio of the eucommia ulmoides leaves in the mixture A to the mixed bacterial liquid is 100: (5-6);
the stirring speed is 80-120r/min, and the stirring time is 8-12 min.
6. The preparation method of the eucommia ulmoides peptide total nutrient powder as claimed in claim 4, wherein in the step (4), the mass ratio of the water for adding water for enzymolysis to the eucommia ulmoides leaves in the mixture A is 4-6:1, the temperature for adding water for enzymolysis is 35-45 ℃, and the time is 4-6 h;
the mass ratio of the water added after enzymolysis to the eucommia ulmoides leaves in the mixture A is 2-4: 1; the reflux extraction time is 2-4 h.
7. The preparation method of the eucommia ulmoides peptide total nutrient powder as claimed in claim 4, wherein in the step (5), the mass ratio of the water to the filter residue I is 5-7: 1; the reflux extraction time is 2-3 h.
8. The method for preparing eucommia ulmoides peptide total nutrient powder according to claim 4, wherein in the step (6), the concentration of the chitosan solution is 1%, and the volume ratio of the chitosan solution to the filtrate III is 1-3: 100;
the standing time is more than 2 hours;
the drying temperature is 65-75 ℃, and the drying time is 6-8 h.
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