CN113755301A - A kind of culture medium intelligent sub-packaging system and application method - Google Patents
A kind of culture medium intelligent sub-packaging system and application method Download PDFInfo
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- CN113755301A CN113755301A CN202111269843.8A CN202111269843A CN113755301A CN 113755301 A CN113755301 A CN 113755301A CN 202111269843 A CN202111269843 A CN 202111269843A CN 113755301 A CN113755301 A CN 113755301A
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- 239000001963 growth medium Substances 0.000 title claims abstract description 45
- 238000000034 method Methods 0.000 title claims abstract description 20
- 238000004806 packaging method and process Methods 0.000 title claims description 12
- 239000007788 liquid Substances 0.000 claims abstract description 78
- 238000000855 fermentation Methods 0.000 claims abstract description 71
- 230000004151 fermentation Effects 0.000 claims abstract description 71
- 229910021642 ultra pure water Inorganic materials 0.000 claims abstract description 26
- 239000012498 ultrapure water Substances 0.000 claims abstract description 26
- 239000012452 mother liquor Substances 0.000 claims abstract description 18
- 238000009826 distribution Methods 0.000 claims abstract description 12
- 238000002360 preparation method Methods 0.000 claims abstract description 11
- 239000002609 medium Substances 0.000 claims abstract description 8
- 238000005086 pumping Methods 0.000 claims abstract description 8
- 238000009434 installation Methods 0.000 claims abstract description 4
- 238000005303 weighing Methods 0.000 claims description 28
- 230000002572 peristaltic effect Effects 0.000 claims description 25
- 239000000463 material Substances 0.000 claims description 17
- 235000015097 nutrients Nutrition 0.000 claims description 16
- 230000007246 mechanism Effects 0.000 claims description 8
- 230000008569 process Effects 0.000 claims description 8
- 238000013519 translation Methods 0.000 claims description 6
- 238000013461 design Methods 0.000 claims description 5
- 238000007789 sealing Methods 0.000 claims description 2
- 238000012856 packing Methods 0.000 claims 3
- 239000002253 acid Substances 0.000 claims 1
- 239000011159 matrix material Substances 0.000 claims 1
- 238000000926 separation method Methods 0.000 abstract description 2
- 230000009286 beneficial effect Effects 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 6
- 238000002156 mixing Methods 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 239000011344 liquid material Substances 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- CMHXYXQNKLCTDV-UHFFFAOYSA-N boric acid;silicon Chemical compound [Si].OB(O)O CMHXYXQNKLCTDV-UHFFFAOYSA-N 0.000 description 2
- 210000004556 brain Anatomy 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 210000001503 joint Anatomy 0.000 description 2
- 239000010413 mother solution Substances 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000013522 chelant Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012527 feed solution Substances 0.000 description 1
- 238000013537 high throughput screening Methods 0.000 description 1
- 238000005304 joining Methods 0.000 description 1
- 239000012092 media component Substances 0.000 description 1
- 238000011177 media preparation Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M99/00—Subject matter not otherwise provided for in other groups of this subclass
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Abstract
本发明公开了一种培养基智能分装系统及应用方法，所述培养基智能分装系统包括料液单元、泵站、控制系统、可移动分装头和发酵设备单元，料液单元连接泵站，泵站连接可移动分装头，控制系统连接有可移动机械臂，可移动分装头安装在可移动机械臂上；控制系统连接并控制泵站和发酵设备单元。其应用方法，包括以下步骤：a.培养基中各组分母液的配置；b.超纯水的准备；c.微量组分的准备；d.发酵罐的安装；e.配方的设置；f.泵头的分配；g.管线的连接；h.培养基的填充。本发明的自动化程度高，操作省时省力；调控精准，利于在高通量平行生物反应器上使用，且本发明可有效的避免人工分液带来的操作误差，提高平行反应器之间的平行性能。
The invention discloses a culture medium intelligent dispensing system and an application method. The culture medium intelligent dispensing system includes a material-liquid unit, a pump station, a control system, a movable dispensing head and a fermentation equipment unit, and the material-liquid unit is connected to a pump The pump station is connected to the movable dispensing head, the control system is connected with a movable mechanical arm, and the movable dispensing head is installed on the movable mechanical arm; the control system is connected to and controls the pumping station and the fermentation equipment unit. The application method includes the following steps: a. the configuration of each component mother liquor in the culture medium; b. the preparation of ultrapure water; c. the preparation of trace components; d. the installation of the fermentation tank; e. the setting of the formula; f . Distribution of pump heads; g. Connection of pipelines; h. Filling of medium. The invention has a high degree of automation, saves time and labor in operation, accurate regulation, and is beneficial to use in high-throughput parallel bioreactors, and the invention can effectively avoid operation errors caused by manual liquid separation, and improve the efficiency between parallel reactors. Parallel performance.
Description
Technical Field
The invention relates to a subpackaging system and an application method, in particular to an intelligent subpackaging system for a culture medium and an application method.
Background
During the process of biological fermentation culture, nutrient substances are required to be continuously provided for cells. The nutrient medium prepared from various nutrient substances is called as culture medium. The culture medium is not only a basic substance for providing nutrition for the cells and promoting the proliferation of the cells, but also a living environment for the growth and the propagation of the cells.
In the process of high throughput screening optimization of most cells, a large number of bioreactors are often required. Each bioreactor needs to be filled with a nutrient medium prior to fermentation, which is composed of multiple components, such as various carbon sources, nitrogen sources, inorganic salts, vitamins, water, and the like. Conventionally, a plurality of nutrients are mixed and prepared in a large container according to the demand of the culture medium of the actual reactor, and the mixture is uniformly stirred and then is distributed to each independent bioreactor.
The disadvantage of this operation is that the operation mode of mixing multiple nutrients in advance in dilution, regardless of the adding sequence of each nutrient component, may cause chemical reaction between partial nutrients, form precipitate or chelate, and cause loss of nutrients; the accuracy of manual split charging after mixing is not enough, potential operation errors exist, and even a small operation error of a micro parallel bioreactor can cause that the parallelism among the reactors is greatly limited. Particularly when the number of reactors is increased and a large amount of medium substrate is required, the volume requirement for a single mixing vessel is large and the uniformity of mixing between nutrients is limited. In short, the conventional culture medium preparation and distribution method is time-consuming and labor-consuming, and the distribution accuracy is also limited.
Therefore, the development of an intelligent culture medium subpackaging system and an application method becomes a problem to be solved by the technical personnel in the field.
Disclosure of Invention
The invention provides an intelligent culture medium subpackaging system and an application method for solving the defects. The system can automatically realize that a plurality of nutrient substances in the culture medium are independently and automatically filled into a specified bioreactor according to a certain sequence and a certain demand.
The above object of the present invention is achieved by the following technical means: an intelligent culture medium subpackaging system comprises a feed liquid unit, a pump station, a control system, a movable subpackaging head and a fermentation equipment unit, wherein the feed liquid unit is connected with the pump station, the pump station is connected with the movable subpackaging head, the control system is connected with a movable mechanical arm, and the movable subpackaging head is arranged on the movable mechanical arm; the control system is connected with and controls the pump station and the fermentation equipment unit.
In the invention, the feed liquid unit is a supply station of a culture medium and is provided with a plurality of feed liquid bottles, and various feed liquids can be prepared with high-concentration mother liquid in advance and are pre-filled into the corresponding feed liquid bottles; the control system is a central brain of the culture medium intelligent subpackaging system and can be designed and allocated to send out corresponding working instructions; the pump station is a power device for conveying the material liquid, and can accurately convey the material liquid in the material liquid bottle according to a corresponding instruction sent by the control system; the movable sub-packaging head is an auxiliary tool, is loaded on a movable mechanical arm on the control system, can be accurately moved to the top end of each fermentation tank, is in butt joint with the fermentation tanks, and conveys the feed liquid conveyed by the pump station to a specified fermentation tank; the fermentation equipment unit is a bearing device of the feed liquid, and bears the culture medium conveyed from the feed liquid unit.
Further, the feed liquid unit comprises feed liquid bottles with various specifications and a micro syringe. The feed liquid bottles with various specifications are used for bearing feed liquid mother liquid with different volumes; the material liquid bottle with large capacity is used for bearing components with large volume, the addition of components with large volume such as ultrapure water can be met, and the ultrapure water can be used for each fermentation tank with constant volume finally; the micro syringe is used for bearing the micro components and meets the requirement of accurate addition of the micro components.
Further, the control system comprises a control cabinet and a human-computer interface; a control system hardware control mechanism is arranged in the control cabinet and connected with the movable mechanical arm; a groove for placing peristaltic pumps is formed in the side face of the control cabinet, and peristaltic pumps with various specifications can be installed to form a pump station; the control computer case top is provided with feed liquid bottle recess and human-computer interface, and human-computer interface is used for carrying out the editor setting of system control logic for present jar body discernment and bootstrap system, the state of different pilot lamps in the interface presents the current characteristic of the fermentation cylinder jar body, is used for carrying out corresponding liquid logic design of joining in marriage: a. an operator configures mother liquor with proper concentration according to actual needs, inputs related information into the system, and calculates the volume required by each mother liquor and the volume of ultrapure water by the control system; b. and the control system compares the precision of each pump in the system with the volume required by each component mother liquor to obtain an optimal distribution scheme. A mechanical arm translation notch is formed in the middle of the control cabinet, the movable mechanical arm penetrates through the mechanical arm translation notch to be connected with a control system hardware control mechanism, and the movable mechanical arm moves transversely or longitudinally in a specified space range according to an instruction sent by a human-computer interface to accurately align the top ends of the fermentation tanks; the movable mechanical arm can be arranged in a plurality of at least one.
Furthermore, the pump station consists of a plurality of peristaltic pumps with different precision specifications and is used for controlling the adding precision of nutrient solutions with different volumes; the pump station comprises a high-flow peristaltic pump, a low-flow peristaltic pump and a micro-syringe pump. Wherein, the high-flow peristaltic pump is mainly distributed to the components with large demand in the formula for use; the micro syringe pump is mainly used for adding trace components; the small-flow peristaltic pump can also be designed into a plurality of different precision gradient ranges so as to better meet the liquid preparation requirement of the culture medium.
Further, first size of portable partial shipment is with fermentation cylinder tank deck head, and portable partial shipment is overhead to be provided with a plurality of filling contact pins, and the aperture of filling the contact pin can set to different specifications to alright in order to satisfy the transport of different volume flow's nutrient solution.
Further, the fermentation equipment unit comprises an intelligent weighing base and a fermentation tank, wherein the intelligent weighing base is provided with a plurality of fermentation tank grooves for accommodating the fermentation tank, the fermentation tank grooves are distributed in a row-by-row manner, and corresponding row number marks and column number marks are specified so as to help quickly identify the position of the fermentation tank; a weighing module is arranged in a fermentation tank groove of the intelligent weighing base and is used for weighing and controlling the liquid filling amount of the fermentation tank; the upper end panel of the intelligent weighing base is provided with a scanner for scanning an identification code at the bottom of the fermentation tank and extracting fermentation tank information; each groove edge of the intelligent weighing base is provided with a status indicator light for identifying the status of the fermentation tank in the corresponding groove, and the different statuses of the indicator lights guide users to perform different operations.
Further, the fermenter comprises a fermenter body; the bottom of the fermentation tank body is provided with an identification code which can be a two-dimensional code or a bar code and is a symbol of the identity of the fermentation tank; the fermentation tank body can be made of a silicon boric acid material and can also be made of a disposable organic plastic material.
The application method of the intelligent culture medium subpackaging system comprises the following steps:
a. preparing mother liquor of each component in a culture medium: according to the experimental formula, all components in the formula are prepared into high-concentration mother liquor and filled into corresponding liquid bottles, in order to reduce the use of the liquid bottles, the components which do not react with each other can be prepared into the same liquid bottle, and trace components can be loaded into a micro-syringe pump;
b. preparation of ultrapure water: preparing ultrapure water to be loaded into an ultrapure water liquid feed bottle, and using the ultrapure water in a constant volume culture medium volume process;
c. preparation of micro-components: the micro-components can be prepared and filled in a micro-syringe;
d. installation of a fermentation tank: removing the tank cover of the fermentation tank, scanning the identification code at the bottom of each fermentation tank through a scanner on the intelligent weighing base, and installing the identification code in a designated fermentation tank groove in the intelligent weighing base according to the indication of an indicator lamp on the intelligent weighing base;
e. setting a formula: in the human-computer interface, inputting relevant information into the system, and calculating by the control system to obtain the volume required by each mother solution and the volume of ultrapure water;
f. dispensing of pump heads: the control system compares the product of the lowest rotating speed and the lowest reliable running time of each pump within an error allowable range with the volume required by each mother liquor according to the lowest pumping volume of each pump in the system to obtain an optimal distribution scheme;
g. connecting pipelines: connecting each liquid feeding bottle with a peristaltic pump with corresponding precision in a system distribution scheme and a movable split charging head of a control system by using a hose;
h. filling of the culture medium: and selecting a reactor needing to be filled with a culture medium in a human-computer interface, and enabling the system to work to sequentially add the feed liquid of each component into the specified reactor according to the volume amount set by the formula.
The advantages of the invention and the prior art are:
1. the invention has high automation degree and time-saving and labor-saving operation; the regulation and control are accurate, and the use on a high-flux parallel bioreactor is facilitated.
2. The intelligent culture medium subpackaging system is applied to the high-flux parallel bioreactor, and the advantages of the intelligent culture medium subpackaging system are more obvious compared with the advantages of manual subpackaging along with the increase of the number of the reactors.
3. The system can design a solution preparation scheme and automatically optimize and match the optimal pump head corresponding to each feed solution.
4. The whole process of adding the culture medium adopts mechanized accurate regulation and control, can effectively avoid operation errors caused by manual liquid separation, and improves the parallel performance between parallel reactors.
Drawings
Fig. 1 is a control flow chart of the present invention.
Fig. 2 is a schematic side perspective view of the present invention.
Fig. 3 is another perspective view of the present invention.
FIG. 4 is a schematic diagram of the structure of the middle fermentation equipment unit of the present invention.
FIG. 5 is a diagram of a screen of a man-machine interface according to the present invention.
FIG. 6 is a flow chart of the application operation of the present invention.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings.
As shown in fig. 1, an intelligent culture medium subpackaging system comprises a material liquid unit 1, a pump station 2, a control system 3, a movable subpackaging head 4 and a fermentation equipment unit 5, wherein the material liquid unit 1 is connected with the pump station 2, the pump station 2 is connected with the movable subpackaging head 4, the control system 3 is connected with a movable mechanical arm 6, and the movable subpackaging head 4 is installed on the movable mechanical arm 6; the control system 3 is connected with and controls the pump station 2 and the fermentation equipment unit 5.
In the invention, the feed liquid unit 1 is a supply station of a culture medium and is provided with a plurality of feed liquid bottles 101, and various feed liquids can be prepared with high-concentration mother liquid in advance and are pre-filled into the corresponding feed liquid bottles 101; the control system 3 is a central brain of the culture medium intelligent subpackaging system and can be designed and allocated to send out corresponding working instructions; the pump station 2 is a power device for conveying the material liquid, and can accurately convey the material liquid in the material liquid bottle 101 according to a corresponding instruction sent by the control system 3; the movable sub-packaging head 4 is an auxiliary tool, is loaded on a movable mechanical arm 6 on the control system 3, can accurately move to the top end of each fermentation tank 502, is in butt joint with the fermentation tanks 502, and conveys the feed liquid conveyed by the pump station 2 to a specified fermentation tank 502; the fermentation equipment unit 5 is a material liquid bearing device, and the fermentation equipment unit 5 bears the culture medium conveyed from the material liquid unit 1.
As shown in fig. 2, 3 and 6, the feed liquid unit 1 includes a plurality of feed liquid bottles 101, 102 and 103, and a micro syringe 105. The feed liquid bottles with various specifications are used for bearing feed liquid mother liquid with different volumes; the liquid bottle 104 with large capacity is used for bearing components with large volume, so that the addition of components with large volume such as ultrapure water can be satisfied, and the ultrapure water can be used for each fermentation tank with constant volume; the micro syringe 105 is used for bearing micro components and meets the requirement of accurate addition of the micro components.
As shown in fig. 2 and 3, the control system 3 includes a control cabinet 301 and a human-machine interface 302; a control system hardware control mechanism 303 is arranged in the control cabinet 301, and the control system hardware control mechanism 303 is connected with the movable mechanical arm 6; a groove 304 for placing peristaltic pumps is formed in the side face of the control cabinet 301, and peristaltic pumps of various specifications can be installed to form a pump station 2; the top of the control cabinet 301 is provided with a feed liquid bottle groove 305 and a human-computer interface 302, the human-computer interface 302 is used for editing and setting system control logic and presenting a tank body identification and guidance system, and the states of different indicator lamps in the interface present the current characteristics of the fermentation tank body 5021. In this embodiment, the man-machine interface 302 may be configured with a corresponding liquid preparation logic design, and the design scheme is as follows:
a. an operator configures mother liquor with proper concentration according to actual needs, inputs the concentration of the mother liquor, target concentration and reaction volume into the system, and calculates the volume required by each mother liquor and the volume of ultrapure water by the control system;
b. the control system compares the product of the lowest rotating speed and the lowest reliable running time of each pump in an error allowable range with the volume required by each mother liquor according to the lowest pumping volume of each pump in the system to obtain an optimal distribution scheme.
c. An example of 4 media components dispensed to four peristaltic pumps is shown in table 1:
as can be seen from the example, the pump 1 is a large-flow pump mainly used for adding a large volume of ultrapure water for use, the component 2, the component 3, and the component 4 cannot ensure the liquid dispensing accuracy if the pump 2 is used, and the component 1 takes too long if the pump 3 or the pump 4 is used. Component 4 may be used with a micro syringe pump. Thus, there is only one possible solution in the figure.
d. If the pump configuration fails to meet the dispensing requirements, the system prompts the user to replace the thinner hose. After the user inputs the hose model of each pump into the system, the system performs the above calculation and distribution again. For minor components, the system will prompt the user to add manually using a micro-syringe pump or using a pipette after calculating the volume.
A mechanical arm translation notch 306 is formed in the middle of the control cabinet 301, the movable mechanical arm 6 penetrates through the mechanical arm translation notch 306 to be connected with the control system hardware control mechanism 303, and the movable mechanical arm 6 moves transversely or longitudinally in a specified space range according to an instruction sent by the human-computer interface 302 to accurately align the top ends of the fermentation tanks 502; the movable mechanical arm 6 may be provided in plurality, at least one.
As shown in fig. 5, the human-machine interface 302 described in this embodiment may present a tank recognition and guidance system:
interface indicator light flashing NT: indicating that the tank body is ready to be put into after being scanned and identified;
interface indicator lights are normally on NO: indicating that the can has been placed;
interface indicator lamp long-off NC: indicating that the can body has not been placed.
As shown in fig. 3, the pump station 2 is composed of a plurality of peristaltic pumps with different precision specifications, and is used for controlling the adding precision of nutrient solutions with different volumes; the pump station 2 comprises a large flow peristaltic pump 201 (limiting the minimum pump-out volume to 50 ml), a small flow peristaltic pump 202 (limiting the minimum pump-out volume to 2-10ml) and a micro syringe pump 203 (limiting the minimum pump-out volume to 0.1 ml). In particular, the minimum pumping volume of the pump station in the embodiment of the invention is limited by the thickness of the feed liquid hose used in the process besides the pump head. The feed liquid hose can be changed according to actual needs to change the minimum pumping volume limit of each pump head. The high-flow peristaltic pump 201 is mainly distributed to components with large demand in the formula for use, and the high-flow peristaltic pump is mainly distributed to ultrapure water for use because the ultrapure water for constant volume of the culture medium has the largest volume demand; the micro syringe pump 203 is mainly used for adding micro components, and the micro syringe pump is started because the peristaltic pump with small flow cannot meet the precision; the small flow peristaltic pump 202 can also be designed to have several different precision gradient ranges to better meet the media dispensing requirements.
As shown in figure 3, the size of the movable subpackaging head 4 is the same as that of the tank top sealing head of the fermentation tank 502, a plurality of filling contact pins are arranged on the movable subpackaging head 4, the hole diameters of the filling contact pins can be set to be different specifications, and therefore conveying of nutrient solution with different volume flow can be met.
As shown in fig. 4, the fermentation equipment unit 5 comprises an intelligent weighing base 501 and a fermentation tank 502, wherein the intelligent weighing base 501 is provided with a plurality of fermentation tank grooves 503 for accommodating the fermentation tank 502, the fermentation tank grooves 503 are distributed in a row-column manner, and corresponding row number marks 504 and column number marks 505 are defined so as to help quickly identify the position of the fermentation tank 502; a weighing module is arranged in a fermentation tank groove 503 of the intelligent weighing base 501 and is used for weighing and controlling the liquid filling amount of the fermentation tank 502; a scanner is arranged on the upper end panel of the intelligent weighing base 501 and used for scanning the identification code 5022 at the bottom of the fermentation tank 502 and extracting information of the fermentation tank 502; the intelligent weighing base 501 is provided with a status indicator lamp 506 on each groove edge for identifying the status of the fermentation tank 502 in the corresponding groove, and the different statuses of the indicator lamps guide users to perform different operations.
The indicator light on the base chassis described in this example is divided into four states:
the status indicator lamp is on for a long time: representing no placement in the fermentor;
the status indicator lamp slowly flashes: representing that the fermentation tank is scanned to be placed in a specified base case groove;
the status indicator lamp is normally on: representing the fermenter which is already put into the code scanning identification;
the status indicator lamp flashes quickly: the representative fermentation tank is directly placed into the groove of the base case without code scanning identification.
Further, the fermentor 502 comprises a fermentor tank 5021; the bottom of the fermentation tank body 5021 is provided with an identification code 5022, the identification code 5022 can be a two-dimensional code or a bar code, and the identification code 5022 is a symbol of the identity of the fermentation tank 502; the fermentation tank 5021 can be made of silicon boric acid or disposable organic plastic.
As shown in fig. 6, the application method of the intelligent culture medium subpackaging system of the invention comprises the following steps:
a. preparing mother liquor of each component in a culture medium: according to the experimental formula, all components in the formula are prepared into high-concentration mother liquor and filled into corresponding liquid material bottles 101, in order to reduce the use of the liquid material bottles 101, components which do not react with each other can be prepared into the same liquid material bottle 101, and trace components can be loaded into a micro-syringe pump 203;
b. preparation of ultrapure water: preparing ultrapure water to be loaded into an ultrapure water liquid feed bottle 101, wherein a valve 7 is arranged at the front end of the ultrapure water liquid feed bottle, and the ultrapure water liquid feed bottle is used as a constant volume culture medium volume in the process;
c. preparation of micro-components: the micro-components may be formulated to fill the micro-syringe 105;
d. installation of fermentor 502: the fermentation tanks 502 are subjected to tank cover removal, the identification codes 5022 at the bottoms of the fermentation tanks 502 are scanned by the scanners on the intelligent weighing bases 501, and the fermentation tanks are arranged in the designated fermentation tank grooves in the intelligent weighing bases 501 according to the indication of the indicator lamps on the intelligent weighing bases 501;
e. setting a formula: in the human-computer interface 302, relevant information is input into the system, and the control system 3 calculates the volume required by each mother solution and the volume of ultrapure water;
f. dispensing of pump heads: the control system 3 compares the product of the lowest rotating speed and the lowest reliable running time of each pump within an error allowable range with the volume required by each mother liquor according to the lowest pumping volume of each pump in the system to obtain an optimal distribution scheme;
g. connecting pipelines: connecting each feed liquid bottle 101 with a peristaltic pump with corresponding precision in a system distribution scheme and a movable sub-packaging head 4 of a control system 3 by using a hose;
h. filling of the culture medium: the reactors to be filled with the culture medium are selected in the human-computer interface 302, and the system starts to work to add the feed liquid of each component into the designated reactors in sequence according to the volume amount set by the formula.
The above description is only an embodiment of the present invention, and not intended to limit the scope of the present invention, and all modifications of equivalent structures and equivalent processes performed by the present specification and drawings, or directly or indirectly applied to other related technical fields, are included in the scope of the present invention.
Claims (8)
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