CN111079456A - Culture medium preparation equipment and method - Google Patents
Culture medium preparation equipment and method Download PDFInfo
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- CN111079456A CN111079456A CN201911192628.5A CN201911192628A CN111079456A CN 111079456 A CN111079456 A CN 111079456A CN 201911192628 A CN201911192628 A CN 201911192628A CN 111079456 A CN111079456 A CN 111079456A
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- 239000001963 growth medium Substances 0.000 title claims abstract description 77
- 238000011177 media preparation Methods 0.000 title claims abstract description 37
- 238000000034 method Methods 0.000 title claims abstract description 21
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- 238000002156 mixing Methods 0.000 claims abstract description 10
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- 238000004891 communication Methods 0.000 claims description 22
- 239000002609 medium Substances 0.000 claims description 14
- 238000001816 cooling Methods 0.000 claims description 12
- 238000010438 heat treatment Methods 0.000 claims description 12
- 239000012092 media component Substances 0.000 claims description 3
- 239000000306 component Substances 0.000 abstract description 107
- 238000002360 preparation method Methods 0.000 abstract description 19
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- QSBNOZODKXUXSP-UHFFFAOYSA-K bismuth;azane;2-hydroxypropane-1,2,3-tricarboxylate Chemical compound N.[Bi+3].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O QSBNOZODKXUXSP-UHFFFAOYSA-K 0.000 description 2
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Images
Classifications
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- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06K—GRAPHICAL DATA READING; PRESENTATION OF DATA; RECORD CARRIERS; HANDLING RECORD CARRIERS
- G06K7/00—Methods or arrangements for sensing record carriers, e.g. for reading patterns
- G06K7/10—Methods or arrangements for sensing record carriers, e.g. for reading patterns by electromagnetic radiation, e.g. optical sensing; by corpuscular radiation
- G06K7/10544—Methods or arrangements for sensing record carriers, e.g. for reading patterns by electromagnetic radiation, e.g. optical sensing; by corpuscular radiation by scanning of the records by radiation in the optical part of the electromagnetic spectrum
- G06K7/10821—Methods or arrangements for sensing record carriers, e.g. for reading patterns by electromagnetic radiation, e.g. optical sensing; by corpuscular radiation by scanning of the records by radiation in the optical part of the electromagnetic spectrum further details of bar or optical code scanning devices
- G06K7/10861—Methods or arrangements for sensing record carriers, e.g. for reading patterns by electromagnetic radiation, e.g. optical sensing; by corpuscular radiation by scanning of the records by radiation in the optical part of the electromagnetic spectrum further details of bar or optical code scanning devices sensing of data fields affixed to objects or articles, e.g. coded labels
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M99/00—Subject matter not otherwise provided for in other groups of this subclass
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
Abstract
The invention relates to the technical field of culture medium preparation, and provides culture medium preparation equipment and a culture medium preparation method, which are used for mixing culture medium components in different packages and comprise the following steps: a barcode scanner or radio frequency identifier; the controller is used for judging whether the component types and the volumes read by the code scanner or the radio frequency identifier are correct or not based on the set culture medium data; the prompter is connected with the controller and sends out a prompting signal when the component type or the component volume read by the code scanner or the radio frequency identifier is incorrect or expired and failed. The components adopted by the culture medium preparation equipment are packaged by the packaging body, and the components in the packaging body are prepared in proportion before water extraction, so that the components in different packaging bodies only need to be directly mixed subsequently, the water adding preparation process of an operator is omitted, and the preparation process is ensured to be easily mastered. In addition, because the controller can be matched with the code scanner or the radio frequency identifier to select the correct packaging body, the preparation of the corresponding culture medium can be realized even if the operation of non-professional personnel is carried out, and the operation is simple.
Description
Technical Field
The invention relates to the technical field of culture medium preparation, in particular to culture medium preparation equipment and a culture medium preparation method.
Background
The culture medium is artificially prepared and is suitable for the growth, separation and identification of microorganisms. The general basic culture medium contains basic nutrient substances such as peptone, beef extract powder, yeast extract powder, sodium chloride, agar and the like. In the culture medium used for clinical and food hygiene tests, it is often necessary to add bacteriostatic agents, indicators, blood, saccharides, etc. to facilitate the separation and identification of specific bacteria, while in the culture medium used for virus culture and vaccine production, it is necessary to add different kinds of amino acids, nucleotides, inorganic salts, growth factors, etc. The complexity of the components of the culture medium and the difference of the stability of the additives directly determine the preparation process and the addition flow of the culture medium, and the targeted control is needed in the actual work.
The culture medium as basic material for microbe research is used in the following state: first, a completely sterile state; secondly, all components in the culture medium are balanced and effective, and the components of the culture medium are not lost or denatured in the preparation process; finally, the preparation time is short to ensure the activity of the microorganisms in the sample to be tested during inoculation, especially the clinical samples and anaerobic bacteria.
In order to make the culture medium reach the state when in use, the culture medium in the prior art is generally prepared and used in a laboratory, which puts high requirements on operators. On the basis, a dry powder type culture medium is proposed in the market, the dry powder type culture medium also needs to be prepared, the preparation process is complex, the components and the adding process thereof are not easy to master, and errors are easy to generate in the actual work to cause experiment failure.
Disclosure of Invention
The present invention is directed to solving at least one of the problems of the prior art or the related art.
One of the objects of the invention is: the culture medium preparation equipment is provided, and the problems that the culture medium preparation process in the prior art has high requirements on operators, and each component and the addition flow thereof are easy to master are solved.
To achieve this object, the present invention provides a medium formulating device for mixing different medium components contained in different packages, comprising:
a code scanner or a radio frequency identifier, the code scanner reads the identification code on the package body, the identification code stores the component information and the component volume information in the package body, or,
the radio frequency identifier reads an electronic tag on the packaging body, and the electronic tag stores component information and component volume information in the packaging body;
the controller is connected with the code scanner or the radio frequency identifier and judges whether the types and the volumes of the components read by the code scanner or the radio frequency identifier are correct or not based on the set culture medium data;
and the prompter is connected with the controller and sends out a prompt signal when the component types or the component volumes read by the code scanner or the radio frequency identifier are incorrect or are expired and failed.
In one embodiment, the package contains one of a coagulant solution, a nutrient solution, and an additive solution.
In one embodiment, the package comprises a first package for containing a coagulant solution, and a second package for containing a nutrient solution and/or a third package for containing an additive solution;
the culture medium preparation equipment further comprises a temperature control chamber, wherein the temperature control chamber is used for placing the first packaging body and adjusting the temperature of the coagulant solution, a heating part and a cooling part are arranged in the temperature control chamber, and the controller controls the heating part and the cooling part.
In one embodiment, the temperature controlled chamber is provided with an electrically controlled door;
the controller is connected with the electric control door, and the controller judges whether the component types and the component volumes read by the code scanner or the radio frequency identifier are correct and controls the electric control door to be opened under the condition that the component types and the component volumes are correct and the component volumes are in a guarantee period.
In one embodiment, the medium formulating device further comprises:
the mounting component is positioned outside the temperature control chamber and used for placing the second packaging body and/or the third packaging body;
the culture medium preparation equipment further comprises a first communication pipe, and the first communication pipe is used for introducing components in the second package body and/or the third package body at the mounting part into the first package body in the temperature control chamber.
In one embodiment, the number of the first communication pipes is multiple, the number of the second package bodies and/or the number of the third package bodies and the number of the first communication pipes are in one-to-one correspondence and are connected with each other, and the controller controls at least one of the first communication pipes to be communicated.
In one embodiment, the prompting device is an alarm, and the prompting signal is an alarm signal.
In one embodiment, the package is a bag.
In order to achieve the object, the present invention provides a medium preparation method comprising:
taking the package body and placing the package body at a code scanner or a radio frequency identifier;
the code scanner reads the identification code on the package body, or the radio frequency identifier reads the electronic tag on the package body;
the controller judges whether the component types and the volumes read by the code scanner or the radio frequency identifier are correct or not;
the type or volume of the component read is incorrect, or the component has exceeded its shelf life, prompting the package to be replaced.
In one embodiment, the method comprises the following steps:
the read component types and the read volume are correct and within the shelf life, and the controller controls the temperature of the temperature control chamber based on the read component types and the read volume.
In one embodiment, a first package containing a coagulant and a second package containing a nutrient solution are placed at a barcode scanner or radio frequency identifier;
the controller reads the coagulator in the first packaging body and the nutrient solution in the second packaging body and judges whether the additives need to be injected or not based on the identification result;
if so, placing the third packaging body containing the additive at a code scanner or a radio frequency identifier;
the controller reads whether the component type and volume in the third package are correct, the read component type or volume is incorrect, or the component type and volume is expired and invalid, and prompts the package to be replaced.
In one embodiment, the controller reads that the component type and volume in the third package are correct and have not expired:
controlling an additive in a third package to be injected into the first package;
controlling the nutrient solution in the second package body to be injected into the third package body to flush the third package body;
and controlling the nutrient solution in the third package body to be injected into the first package body.
The technical scheme of the invention has the following advantages: according to the culture medium preparation equipment, the adopted components are packaged by the packaging body, and the components in the packaging body are prepared in proportion before water extraction, so that the components in different packaging bodies are directly mixed in the follow-up process, the water adding preparation process of operators is omitted, and the preparation process is ensured to be easily mastered. In addition, because the controller can be matched with the code scanner or the radio frequency identifier to select the correct packaging body, the preparation of the corresponding culture medium can be realized even if the operation of non-professional personnel is carried out, and the operation is simple.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a schematic flow chart of one method of preparing a culture medium according to an embodiment of the present invention;
FIG. 2 is a schematic flow diagram of another method of media formulation in an embodiment of the invention.
Detailed Description
The embodiments of the present invention will be described in further detail with reference to the drawings and examples. The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention.
In the description herein, references to the description of the term "one embodiment," "some embodiments," "an example," "a specific example" or "some examples" or the like are intended to mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this description, a schematic representation of the above terms does not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, various embodiments or examples and features of different embodiments or examples described in this specification can be combined and combined by one skilled in the art without contradiction.
In the description of the present invention, it should be noted that the terms "center", "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "connected" and "connected" are to be interpreted broadly, e.g., as being fixed or detachable or integrally connected; can be mechanically or electrically connected; may be directly connected or indirectly connected through an intermediate. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
First, the medium preparation apparatus of the present invention is applied to a package provided with an identification code and/or an electronic tag. That is, the components of the medium are encapsulated by the packaging. Different media components are packaged in different packages. Furthermore, by the culture medium preparation device of the embodiment of the invention, the mixing of the components in different packages is realized to prepare the required culture medium.
According to an embodiment of the present invention, a media preparation apparatus is provided for mixing different media components contained in different packages, comprising a controller, and further comprising a barcode scanner or radio frequency identifier.
Wherein, when the culture medium preparation device comprises the code scanner and the controller, the corresponding package body is provided with the identification code. The identification code may be a two-dimensional code or a bar code. The identification code stores the component information and the component volume information in the package body. Wherein, the component information mainly refers to the component types, and the component volume information mainly refers to the amount of the solution contained in the package. The code scanner reads the identification code on the package and sends the read information to the controller. The controller can judge the type of the component currently contained in the package and the volume of the component based on the information read by the scanner.
Similarly, when the culture medium preparation device comprises the radio frequency identifier and the controller, the corresponding package body is provided with the electronic tag. The electronic tag stores component information and component volume information in the package. The radio frequency identifier reads the electronic tag on the package body and sends the read information to the controller. The controller can judge the type of the component currently contained in the package and the volume of the component based on the information read by the radio frequency identifier.
Specifically, the controller records set culture medium data, including the components and component ratios required by the set culture medium. The controller is connected with the code scanner or the radio frequency identifier, and judges whether the information read by the current code scanner or the radio frequency identifier is matched with the data recorded in the controller or not based on the set culture medium data, namely whether the component types and the volumes read by the code scanner or the radio frequency identifier are correct or not.
For example, one hundred media are entered in advance in the controller, and the components required to prepare each media and the ratio between the components are entered. On this premise, a certain medium can be selected, and the kinds of components and the component ratios for preparing the medium are also determined. On the basis, in the preparation process of the culture medium preparation equipment, the components in the taken package are read through a code scanner or a radio frequency identifier, and the controller compares the read component information with the component information stored in the controller to judge whether the components in the currently taken package are correct or not.
And the prompter is connected with the controller and sends out a prompting signal when the component type or volume read by the code scanner or the radio frequency identifier is incorrect or expired and failed.
This kind of culture medium preparation equipment, the component that its adopted passes through the packing body encapsulation to the component in the middle of the packing body and the water according to the proportion before preparing, and then follow-up only need directly with the component in the middle of the different packing bodies mix can, saved operating personnel and added water preparation process, guarantee that the preparation flow masters easily. In addition, because the controller can be matched with the code scanner or the radio frequency identifier to select the correct packaging body, the preparation of the corresponding culture medium can be realized even if the operation of non-professional personnel is carried out, and the operation is simple.
In one embodiment, the medium formulating device comprises a display screen with an interactive interface thereon. The media to be formulated may be selected based on the interactive interface. On the basis, the interactive interface sends a selection signal to the controller, and the controller calls the component type and the component distribution ratio of one culture medium based on the selection signal.
Furthermore, the controller is provided with a comparison module. The controller calls the component type and the component distribution ratio of one culture medium based on the selection signal and sends the data of the component type and the component distribution ratio to the comparison module. In addition, the data representing the component information and the component volume information read by the code scanner or the radio frequency identifier is also sent to the comparison module. Furthermore, the comparison module can judge whether the components and the volumes of the components in the currently taken packaging body are correct by judging whether the two sets of data are the same.
Even if the type of the culture medium is not selected in advance in the preparation process of the culture medium, the controller can judge whether the components in the different taken packages are matched or not based on the data recorded in advance. For example, a scanner or radio frequency identifier reads a nutrient solution first, followed by a coagulant solution. The controller may determine whether a subsequently fetched coagulant solution may be matched with the current nutrient solution for preparing the culture medium based on the nutrient solution read first.
Furthermore, the above mentioned identification code or electronic label may also store the shelf life of the component. Furthermore, the code scanner or the radio frequency identifier can also judge whether the components in the current packaging body are overdue or not by reading the identification code or the electronic tag.
Since the components of the culture medium can be divided into a coagulant, a nutrient and an additive, one of a coagulant solution (or gel), a nutrient solution and an additive solution can be contained in the package. Wherein the coagulant solution is obtained by mixing coagulant and water according to a set proportion. Similarly, the nutrient solution is obtained by mixing a nutrient and water according to a set proportion; the additive solution is obtained by mixing an additive and water according to a set proportion. (expression is correct)
Under the premise, the corresponding culture medium can be prepared by only mixing at least two of the coagulant solution, the nutrient solution and the additive solution.
In one embodiment, the main component of the coagulant solution is agar, in which case the coagulant solution is also agar gel. The melting point of the agar gel is about 90-95 ℃, the freezing point is 40-45 ℃, and the characteristic determines that the agar can be used as a coagulant of a microorganism culture medium to prepare the culture medium into a required shape at a lower temperature.
In one embodiment, the package comprises a first package for containing a coagulant solution. In addition, the package may further include a second package for containing a nutrient solution, or a third package for containing an additive solution, or may include both the second package and the third package. That is, the package includes the second package and/or the third package in addition to the first package. Among them, since a nutrient solution is necessary for the culture medium in most cases, a second package for containing the nutrient solution is necessary in most cases. Wherein, because most of culture medium all needs coagulant solution, and coagulant solution generally need to go through heating process and cooling process in proper order, therefore culture medium preparation equipment still includes the control by temperature change room, is used for placing first packing body. The temperature control chamber is internally provided with a heating part and a cooling part, and the controller controls the heating part and the cooling part so as to regulate the temperature of the coagulant solution.
Specifically, the controller acquires the information of the coagulant solution in the first package body through the code scanner or the radio frequency identifier, and controls the heating part and the cooling part in the temperature control room based on the information, so that the temperature in the temperature control room meets the requirements of melting and cooling the coagulant in the first package body.
Wherein, the temperature control room can adopt any shape and material, only need to form a relative confined space to satisfy the holding of first packing body and temperature regulation demand can. The heating and cooling components may take any of the structural forms disclosed in the prior art, as long as the heating and cooling requirements are met, respectively.
In one embodiment, the temperature controlled chamber is provided with an electrically controlled door; the controller is connected with the electric control door, and the controller controls the electric control door to be opened under the condition that the controller judges that the component types and the volumes read by the code scanner or the radio frequency identifier are correct. Therefore, when the correct first packaging body is taken, the electric control door is automatically opened, so that the first packaging body is conveniently placed into the temperature control chamber; when the wrong first packaging body is taken, the electric control door cannot be opened at the moment, and misoperation can be further prevented.
The electric control door can adopt any structural form disclosed in the prior art as long as the opening and closing can be realized based on the control signal of the controller.
In one embodiment, the medium formulating device further comprises a mounting member. The mounting component is positioned outside the temperature control chamber and used for placing a second packaging body. Wherein, the installation component can be the mount pad of setting outside the control by temperature change room, also can be the mounting groove of setting at the control by temperature change room top, still can be for fixing the installing support at the control by temperature change room lateral wall, and concrete structural style is unrestricted, as long as can satisfy the installation of second packing body can. When the second packaging body is provided with the hanging hole, the mounting part can also be a hook positioned outside the temperature control chamber.
Further, the culture medium preparation equipment also comprises a first communication pipe, and the first communication pipe is used for introducing components in the second packaging body at the mounting part into the first packaging body in the temperature control chamber. The first communicating pipe is connected with an electric control pump, and the electric control pump pumps the components in the second packaging body into the first packaging body under the control of the controller.
For example, when the code scanner or the radio frequency identifier reads the component information in the package, the controller controls the temperature control temperature and the operation time of the electrically controlled pump based on the component information.
Further, pipe joints are arranged at two ends of the first communication pipe, and the pipe joints are correspondingly arranged on the first packaging body and the second packaging body, so that the first communication pipe and the packaging bodies (including the first packaging body and the second packaging body) are connected. The pipe joint can realize the sealing connection between first connecting pipe and the packing body to can guarantee the quick assembly disassembly between first connecting pipe and the packing body.
The first end of first connecting pipe is located inside the temperature control room, and then when placing first packing body into the middle of the temperature control room, can realize being connected between first packing body and the first connecting pipe. The second end of the first communication pipe is located outside the temperature control chamber and located at the installation part, and therefore when the second packaging body is installed on the installation part, the second packaging body and the first communication pipe can be connected.
In one embodiment, the number of the first communicating pipes is multiple, the number of the second packing bodies is one-to-one corresponding to the number of the first communicating pipes and is connected with the first communicating pipes, and the controller controls at least one of the first communicating pipes to be communicated. Therefore, when the number of the second packaging bodies is more than one, the controller injects the nutrient solution in at least one second packaging body into the coagulant solution. Specifically, the controller may select the first communication pipe to be communicated based on the kind of the coagulant solution.
Of course, when the packing body includes the third packing body, then the mounting part needs to be used for placing the third packing body, and the first communication pipe needs to communicate the third packing body with the first packing body, and the number of the third packing bodies needs to correspond to and be connected to the first communication pipe one-to-one.
When the package body includes both the second package body and the third package body, then the mounting member needs to satisfy the placement of the second package body and the third package body at the same time. And the first communicating pipe satisfies the communication between the first packaging body and the third packaging body, and between the second packaging body and the third packaging body. The sum of the number of the second package and the third package is the same as that of the first package.
In one embodiment, the first packaging body is packaged through the second communication pipe, and the controller controls the second communication pipe to be connected and disconnected.
Wherein, first communicating pipe and second communicating pipe all can adopt the hose, and then the installation of being convenient for.
In one embodiment, the first communicating pipe and the second communicating pipe can both adopt latex hoses, and the first communicating pipe and the second communicating pipe can both adopt a hose capable of cooperating with a peristaltic pump to transfer liquid in the second packaging body to the first packaging body. Wherein, the peristaltic pump is also one of the components of culture medium preparation equipment, and the function is mainly to realize liquid transfer and quantitative partial shipment after the preparation in the preparation process.
In one embodiment, the alarm is an alarm. The alarm is connected with the controller, and the controller controls the alarm to send out an alarm signal when judging that the component types or the volumes read by the code scanner or the radio frequency identifier are incorrect or are expired and failed. Therefore, when the components in the taken packaging bodies are different from the components recorded in advance by the controller, or when the components in the taken packaging bodies are not matched, the alarm can remind an operator to replace the packaging bodies.
Of course, the prompter may take other forms. For example, the prompter may also be a display, and the components in the current packaging body or the volume errors of the components are displayed on the display, so as to remind an operator to replace the current packaging body.
In one embodiment, the package is a bag. Sterile bags can be selected for the packaging bag, so that the components of the culture medium can be stored in a sterile environment. Moreover, the packaging bag is preferably selected from soft packaging bags, so that the packaging bag is convenient to transport and store.
In one embodiment, the preparation time, type and preparation volume of the culture medium can be recorded by the culture medium preparation equipment to form an electronic document, so that the laboratory working process can be traced and recorded.
According to an embodiment of the present invention, referring to fig. 1, a method for preparing a culture medium by using the above culture medium preparing apparatus comprises:
s1, taking the package and placing the package at a code scanner or a radio frequency identifier; wherein the package comprises the first package and the second package mentioned above.
S2, reading the identification code by the code scanner, or reading the electronic tag by the radio frequency identifier; in the process, the component type and volume information can be obtained by the code scanner or the radio frequency identifier and sent to the controller.
And S3, the controller judges whether the component types and volumes read by the scanner or the radio frequency identifier are correct.
S4, the read component type or volume is incorrect, or has expired and expired, prompting the package to be replaced, and S2 to S3 are repeated until the read component type and volume are correct.
Referring to fig. 2, the method for preparing the culture medium further comprises:
s5, the read component types and the read volume are correct, and the controller controls the temperature of the temperature control chamber based on the read component types and the read volume; wherein, the controller can adjust the temperature of the temperature control chamber by controlling the heating component and the cooling component.
In one embodiment, the method for preparing culture medium using the culture medium preparing apparatus described above, in S1, the first package and the second package are taken and placed at a barcode scanner or an rfid, and if it is determined that the component types and volumes of the first package and the second package are correct and there is no expiration failure, it can be further determined whether the additive needs to be injected.
And if not, directly heating the coagulant and mixing the coagulant and the nutrient solution by using the culture medium preparation equipment to prepare the culture medium.
If so, further placing a third package at the position of the code scanner or the radio frequency identifier; the code scanner reads the identification code on the third packaging body, or the radio frequency identifier reads the electronic tag on the packaging body; the controller judges whether the type and the volume of the additive in the third packaging body read by the code scanner or the radio frequency identifier are correct or not; the type or the volume of the read additive is incorrect or the read additive is expired and fails, and the package body is prompted to be replaced; the additive in the third package is controlled to be injected into the first package when the read additive type and volume are correct and the aging failure does not exist.
The controller further controls the nutrient solution in the second package to be injected into the third package to flush the third package, since the third package may have a small amount of additive residue therein. Thereafter, the controller controls the nutrient solution (carrying possible residual additives) in the third package to be injected into the first package.
Taking a BS agar (bismuth sulfite agar) medium as an example, the bismuth sulfite agar medium contains an ammonium bismuth citrate component, and bismuth citrate is not stable enough at room temperature and can be added as an additive for use.
When 500 ml of bismuth sulfite agar medium is prepared by using the culture medium preparation equipment of the embodiment of the invention:
200 ml of concentrated agar was contained in the first package, 297.5 ml of a nutrient solution was contained in the second package, and 2.5 ml of an ammonium bismuth citrate solution was contained in the third package.
The culture medium preparation equipment reads 200 ml of concentrated agar and 297.5 ml of nutrient solution;
on the basis, when the culture medium preparation equipment reads 2.5 ml of bismuth ammonium citrate solution, the controller judges that the read additive is correct;
the controller controls 250 ml of nutrient solution in the second package to be injected into the first package and mixed with 200 ml of concentrated agar;
further, the controller controls 2.5 ml of bismuth ammonium citrate solution in the third package body to be injected into the first package body;
furthermore, the controller controls the nutrient solution to be injected into the third package body for multiple times to wash the third package body, and the washing liquid is added into the first package body through a pipeline to ensure that no additive residue exists in the third package body;
and finally, the controller controls the residual nutrient solution in the second packaging body to be injected into the first packaging body to achieve the effect of flushing the pipeline, so that no residue of the additive is left in the third packaging body and a communicating pipe between the third packaging body and the first packaging body. The above embodiments are merely illustrative of the present invention and are not to be construed as limiting the invention. Although the present invention has been described in detail with reference to the embodiments, it should be understood by those skilled in the art that various combinations, modifications or equivalents may be made to the technical solution of the present invention without departing from the spirit and scope of the technical solution of the present invention, and the technical solution of the present invention is covered by the claims of the present invention.
Claims (12)
1. A media preparation apparatus for mixing different media components contained in different packages, comprising:
a code scanner or a radio frequency identifier, the code scanner reads the identification code on the package body, the identification code stores the component information and the component volume information in the package body, or,
the radio frequency identifier reads an electronic tag on the packaging body, and the electronic tag stores component information and component volume information in the packaging body;
the controller is connected with the code scanner or the radio frequency identifier and judges whether the types and the volumes of the components read by the code scanner or the radio frequency identifier are correct or not based on the set culture medium data;
and the prompter is connected with the controller and sends out a prompt signal when the component types or the component volumes read by the code scanner or the radio frequency identifier are incorrect or are expired and failed.
2. The media preparation apparatus of claim 1, wherein the package contains one of a coagulant solution, a nutrient solution, and an additive solution.
3. The medium formulating device according to claim 2, wherein the package body includes a first package body for containing a coagulant solution, and a second package body for containing a nutrient solution and/or a third package body for containing an additive solution;
the culture medium preparation equipment further comprises a temperature control chamber, wherein the temperature control chamber is used for placing the first packaging body and adjusting the temperature of the coagulant solution, a heating part and a cooling part are arranged in the temperature control chamber, and the controller controls the heating part and the cooling part.
4. The medium formulating device according to claim 3, wherein the temperature controlled compartment is provided with an electrically controlled door;
the controller is connected with the electric control door, and the controller controls the electric control door to be opened under the condition that the controller judges that the component types and the volumes read by the code scanner or the radio frequency identifier are correct.
5. The media preparation apparatus of claim 3, further comprising:
the mounting component is positioned outside the temperature control chamber and used for placing the second packaging body and/or the third packaging body;
the culture medium preparation equipment further comprises a first communication pipe, and the first communication pipe is used for introducing components in the second package body and/or the third package body at the mounting part into the first package body in the temperature control chamber.
6. The medium preparing apparatus according to claim 5, wherein the number of the first communication pipes is plural, the number of the second package and/or the third package is one-to-one corresponding to and connected to the first communication pipes, and the controller controls at least one of the first communication pipes to be conducted.
7. The media preparation apparatus of any one of claims 1-6, wherein the indicator is an alarm and the indicator signal is an alarm signal.
8. The medium formulating device according to any one of claims 1 to 6, wherein the packaging body is a packaging bag.
9. The method for preparing culture medium by the culture medium preparing device according to any one of claims 1 to 8, comprising:
taking the package body and placing the package body at a code scanner or a radio frequency identifier;
the code scanner reads the identification code on the package body, or the radio frequency identifier reads the electronic tag on the package body;
the controller judges whether the component types and the volumes read by the code scanner or the radio frequency identifier are correct or not;
the type or volume of the read component is incorrect or expired and expired, and the package body is prompted to be replaced.
10. The method of claim 9, comprising:
the read component types and volumes are correct, and the controller controls the temperature of the temperature control chamber based on the read component types and volumes.
11. The method for preparing culture medium by the culture medium preparation device according to claim 9, wherein a first package containing a coagulant and a second package containing a nutrient solution are placed at a barcode scanner or a radio frequency identifier;
the controller reads the coagulator in the first packaging body and the nutrient solution in the second packaging body and judges whether the additives need to be injected or not based on the identification result;
if so, placing the third packaging body containing the additive at a code scanner or a radio frequency identifier;
the controller reads whether the component type and volume in the third package are correct, the read component type or volume is incorrect, or the component type and volume is expired and invalid, and prompts the package to be replaced.
12. The method of claim 11, wherein the controller reads that the components in the third package are of the correct type and volume and have not expired:
controlling an additive in a third package to be injected into the first package;
controlling the nutrient solution in the second package body to be injected into the third package body to flush the third package body;
and controlling the nutrient solution in the third package body to be injected into the first package body.
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