CN113749088A - Peony cut flower vase preservative solution - Google Patents
Peony cut flower vase preservative solution Download PDFInfo
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- CN113749088A CN113749088A CN202111182196.7A CN202111182196A CN113749088A CN 113749088 A CN113749088 A CN 113749088A CN 202111182196 A CN202111182196 A CN 202111182196A CN 113749088 A CN113749088 A CN 113749088A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N3/00—Preservation of plants or parts thereof, e.g. inhibiting evaporation, improvement of the appearance of leaves or protection against physical influences such as UV radiation using chemical compositions; Grafting wax
- A01N3/02—Keeping cut flowers fresh chemically
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Abstract
The invention belongs to the field of cut flower preservative solution, and particularly relates to peony cut flower vase preservative solution. The peony cut vase preservative solution provided by the invention contains sucrose, sodium hypochlorite, kynurenine and the balance of water. The peony cut flower preservative solution has no pollution to human bodies and the environment, and is safe and harmless to the human bodies and the environment compared with the prior preservative containing silver ions, preservative containing 8-HQ and the like which are often used as preservative components. The peony cut flower preservative solution is prepared by mixing 1-MCP (sodium chloride-methyl pyrrolidone), ClO (sodium chloride-sodium chloride) and ClO (potassium chloride-sodium chloride) in a mode of bottle-inserting preservative solution2The gas is used as a fresh-keeping component more quickly and conveniently.
Description
Technical Field
The invention belongs to the field of cut flower preservative solution, and particularly relates to peony cut flower vase preservative solution.
Background
The cut flower preservative solution is generally a liquid for preserving fresh cut flowers by adopting a chemical preservative, and is most commonly applied in production at present. The use of the preservative solution can help cut flowers to keep the ornamental quality of the cut flowers for a long time after being harvested, delay aging and prolong the service life of vase. The commodity cut flower is subjected to links such as harvesting, transportation, storage and sale, and in order to reduce loss, the commodity cut flower is generally subjected to fresh-keeping treatment at different stages. The method can be divided into pretreatment liquid and bottle insert liquid according to different use periods after the picking.
In recent years, the study on the cut peony flowers for preservation has been advanced to a certain extent, 8-hydroxyquinoline (8-HQ) and silver nitrate (AgNO)3) Silver Thiosulfate (STS), 1-methylcyclopropene (1-MCP) and the like are used as preservatives to be applied to cut flower preservation, and obvious effects are achieved. However, these effective fresh-keeping ingredients are either toxic to the environment and human body or inconvenient for use as gas.
Results of the Japey (Japey et al, 2006) study show that: the vase solution (3% sucrose +0.2g/LHQS +2mmo1 STS) sucrose +0.2g/LHQS +2mmo1 STS) can obviously prolong the vase life of the Luoyang red cut flower, improve the water balance value and the flower diameter, reduce the fresh weight loss and the ethylene release, and improve the vase ornamental quality of the Luoyang red cut flower. However, 8-hydroxyquinoline (8-HQ) and its derivative salts, 8-hydroxyquinoline citrate (8-HQC) and 8-hydroxyquinoline sulfate (8-HQS), contain toxicity, and among the precancerous substance list preliminary reference published by the international cancer research institution of the world health organization in 2017, 8-HQ is in the 3 kinds of carcinogenic substance list, so that attention should be paid to the safety problem of the use.
The 1.0 mul/L1-MCP is adopted for treatment for 6 hours, so that the opening process of the 'Luoyang red' cut flowers is delayed, and the vase life of the cut flowers is prolonged. Studies on the water balance value showed that 1-MCP treatment improved the water balance of cut flowers and delayed the water balance value to 0 (jasper et al, 2007). Adding chlorine dioxide (0, 25, 50, 100 mg/LClO) with different mass volume fractions into peony 'Luoyang red' bottle insert liquid (2017)2). The results show that 50mg/L of ClO is added into the basic bottle-insert liquid (2% of sucrose, 200mg/L of citric acid, 100mg/L of 8-hydroxyquinoline and 25mg/L of salicylic acid)2The treatment can effectively prolong the vase life and the optimal ornamental period of the peony cut flower. However, 1-MCP and ClO2When the gas is used as the preservative, the gas is injected into water or directly adoptedThe treatment and the flower cutting are inconvenient, and the popularization and the use in the commercial production are difficult.
In addition, STS and fresh-keeping components containing silver ions are prohibited in Europe because they contain heavy metals and the used waste liquid causes environmental pollution.
Disclosure of Invention
In order to solve the problems, the invention provides a peony cut flower vase preservative solution.
The peony cut vase preservative solution contains sucrose, sodium hypochlorite, kynurenine and the balance of water.
In a preferred embodiment of the invention, the peony cut vase preservative solution contains 1-5% of sucrose, 0.1-1.0% of sodium hypochlorite and 25-125mg/L of kynurenine.
In another preferred embodiment of the invention, the peony cut vase preservative solution contains 2% of sucrose, 0.5% of sodium hypochlorite and 125mg/L of kynurenine.
The invention also provides a method for prolonging the vase life of the peony cut vase, which comprises the following steps:
1) putting the peony cut flower into distilled water for re-shearing, and obliquely shearing the base of the flower branch for 45 degrees under water by using special gardening scissors with sharp knife edges;
2) making each cut flower 15-25cm from the base to the top of the stem, and rehydrating for 1-2 h;
3) and inserting the rehydrated peony cut flower into the vase preservative solution.
The peony cut flower preservative solution has no pollution to human bodies and environment, and is compared with the preservative containing silver ions, the preservative containing 8-HQ and the like which are frequently used as preservative components.
The peony cut flower preservative solution is prepared by mixing 1-MCP (sodium chloride-methyl pyrrolidone), ClO (sodium chloride-sodium chloride) and ClO (potassium chloride-sodium chloride) in a mode of bottle-inserting preservative solution2The gas is used as a fresh-keeping component more quickly and conveniently.
Drawings
FIG. 1 shows the effect of different treatments on the flowering grade and the rate of change of the flower diameter of cut flowers of peony.
FIG. 2 shows the effect of different treatments on the fresh weight change and the water balance value of cut flowers of peony.
FIG. 3 shows the effect of different treatments on SOD, POD, CAT content and ethylene release rate during bottle insertion in peony cut flower petals.
Detailed Description
The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention.
Example 1
1 materials and methods
1.1 test materials and treatments
The experiment selects ethylene sensitive type 'Luoyanghong' peony, all comes from the base of the lotus and the peony, robust flowering branches with 2-3 pieces of compound leaves, 30cm long flowering branches and 1-level flowering index (Guoshengwen, 2004) are collected, and are transported back to the laboratory within 12 hours. During transportation, 1 bundle of flowers is wrapped by wet newspaper and placed into a foam box, ice blocks are placed around the foam box, thick paper boards are used for separating the flowers and the ice blocks, and the low temperature is kept.
The cut flowers are immediately put into distilled water for re-shearing after being transported back to a laboratory, the base parts of the flower branches are obliquely sheared by 45 degrees under water by using special gardening scissors with sharp knife edges so as to be beneficial to the absorption of water, each cut flower is about 20cm from the base part to the top part of a flower stem, the cut flowers are rehydrated for 1 hour for standby, the rest leaves are removed, and only one small leaf at the top end is reserved so as to reduce the evaporation of the water.
The trial consisted of 4 treatments (table 1), 10 replicates each, three replicates each. The flower branches were inserted into 250mL jars, respectively, and the bottle mouths were covered with preservative films to prevent evaporation of water and labeled on the bottles. 100mL of preservative solution was added to each jar. And observing morphological characteristics of the seeds at 0h, 6h, 12h, 24h, 48h, 72h and 96h during the bottle inserting period from the day of picking, recording the maximum flower diameter value, fresh weight and the like, and sampling for measuring physiological indexes such as protective enzyme activity and Malondialdehyde (MDA) content.
This experiment is carried out in Beijing university garden institute laboratory, and indoor temperature: 20-23 ℃, and for air humidity: 50-60% of the total light is indoor scattered light.
TABLE 1 different treatment bottle insert formulations
1.2 index measurement and method
And (4) taking a picture of the cut flower during the vase inserting period, observing and recording other morphological characteristics such as the opening grade, the diameter and the like of the flower, and marking the end of vase inserting as the loss of ornamental value of the wilting or petal shedding (grade is marked as 6) of the flower branches. And calculating the optimal viewing period and the bottle inserting service life after the bottle inserting is finished.
Flower opening grade: the patency level of flowers refers to the method of gour went (2004).
The flower diameter is changed: the maximum diameter of the flower is taken at a fixed time every day by measuring with a vernier caliper.
Fresh weight change rate: weighing the quality of the flowering branch once every 12h from bottle insertion by adopting a weighing method, calculating the difference between the quality of the day and the quality of the flowering branch on the first day, and calculating the ratio of the difference to the quality of the flowering branch on the first day, namely the fresh weight change rate of the cut flower. The fresh weight change rate is calculated by the formula: the fresh weight change rate is (fresh weight of flowering branch on the day-fresh weight of initial flowering branch)/fresh weight of initial flowering branch x 100%.
Water balance value: the water absorption and water loss of the flowering branch are measured by a weighing method, and the water balance value is calculated. The calculation formula is as follows: water uptake (daily flower vase + solution mass) - (one day later flower vase + solution mass); the water loss is equal to (the current day vase + solution + flower branch quality) - (the latter day vase + solution + flower branch quality); water balance value is water absorption-water loss.
The petals are quickly frozen by liquid nitrogen and then frozen in an ultra-low temperature refrigerator at-80 ℃ for analyzing the change of physiological and biochemical indexes of the petals in the opening and aging processes of peony cut flowers. The antioxidant protection enzyme SOD activity, and Malondialdehyde (MDA) content were determined with reference to the method of king chemical quini (king chemical quini, 2006).
Data were statistically processed using Microsoft Office Excel 2003 and SPSS13.0 software.
2 results and analysis
Effect of 2.1L-Cannurenine on ornamental quality during vase of Luoyang Red cut bottles
As is clear from Table 2, 75 mg. L-1、125mg·L-1Compared with the CK control, the KYN treatment obviously prolongs the service life of the cut flowers to 0.55d and 0.77d respectively, wherein the service life is 125 mg.L-1The time for KYN treated peony cut flowers to reach the maximum flower diameter was significantly delayed by 26.4h compared to control CK.
TABLE 2 Effect of different treatments on peony cut flower patency and senescence
As can be seen from FIG. 1A, CK in the initial 48h of vial insertion proceeded significantly faster than the respective treatments. CK reached the fourth stage at the 12h flowering index of the vase, whereas treatment K3 reached the fourth stage at the 48h flowering index of the vase, delayed by 36h compared to the control, significantly delaying the opening process of the cut flowers. The open course of CK and other treatments was significantly slowed after 48 h.
The change rate of the flower diameter of the peony cut flower generally shows a trend of ascending first and then descending (fig. 1B), the flower branches quickly absorb water in the earlier stage of cutting the flower vase, the flower rapidly opens, so the change of the flower diameter is rapid, the whole body shows a rapid ascending trend, then the flower loses water and withers, the flower diameter becomes smaller, and the change rate of the flower diameter gradually decreases. The rate of change of flower diameter for control CK peaked at 60h, and the peak rate of change of flower diameter for treatment K2 also peaked at 60h, but at this time the rate of change of flower diameter was significantly higher than for control.
Influence of 2.2L-kynurenine on the fresh weight change and moisture balance value of Luoyang red cut flower vase
As shown in fig. 2A, the fresh weight of the cut peony flower branches tends to increase first and then decrease. The fresh weight change of the processed cut flowers reaches the maximum value after the cut flowers are inserted in the bottle for 60 hours, and then the fresh weight change begins to decline; compared with the control CK, the time of peak arrival of each treatment is delayed by 24 hours, and the fresh weight change is higher than that of the control, which shows that the application of KYN with different concentrations can enhance the water loss stress tolerance of the cut flowers to a certain extent. Among the treatments, the fresh weight change of treatment K2 was always at a high level at the early stage of bottle insertion, as shown in the TableMing 75 mg.L-1The KYN treatment of concentration has the effect of promoting the water absorption of the flowering branch, and is favorable for maintaining the fresh quality of the flowering branch.
The water balance values of the peony cut flower branches during the bottle-cutting showed a tendency of continuously decreasing (fig. 2B). The water balance values of each treatment including the control were positive during 18h before bottle insertion, and then gradually decreased to negative values. The water absorption capacity of the peony cut flowers in 18h in the initial stage of bottle insertion is larger than the water loss capacity, and the water loss capacity is larger than the water absorption capacity along with the increase of the bottle insertion time, so that the water loss stress is increased. In each treatment, the K3 moisture balance value is the highest, the control moisture balance value reaches 0 in 24h, the flower ornamental quality reaches the best, then the moisture balance value is reduced, the moisture balance values in 24h-60h are all smaller than 0, the water loss of the flower is larger than the water absorption amount, and the wilting starts, while the moisture balance value in 60h of the treatment K3 is a positive value, and the time of occurrence of a negative value is the latest. Explanation 125 mg. L-1KYN can better maintain the water holding capacity of flower branches and delay the aging process of cut flowers.
Effect of 2.3L-kynurenine on SOD, POD and CAT during the vase phase of the Luoyang Red cut vase
The overall SOD activity during vial insertion tended to increase first and then decrease (fig. 3A). Compared with CK control, the reference SOD activity peak appears at 12h after bottle insertion, the occurrence time of SOD activity peak can be delayed by KYN treatment with different concentrations, and 75 mg.L-1The KYN treatment postpones the peak appearance time of SOD activity to 36h, and the SOD activity is highest at the moment.
A tendency that POD activity increases slowly and then decreases rapidly (FIG. 3B). After the bottles are inserted for 48h, the POD activity peak values are reached by KYN treatment with different concentrations, the treated K1 and the control CK have similar variation trend, and the POD activity is higher than that of the control when the peak value is reached at the later stage of inserting the K1 bottles.
The CAT activity tended to increase rapidly and then decrease gradually (FIG. 3C). 75 mg. L-1KYN treatment significantly improved CAT activity.
Effect of 2.4L-kynurenine on the amount of ethylene released during vase of a Luoyang Red cut vase
The control group cut flower had an ethylene release peak for 12h and 60h respectively. Different concentrations KYN in the bottle pre-insertion period obviously postpone the appearance of the first peak value6h, and 125 mg.L-1KYN has a significantly lower ethylene release rate than other treatments. The ethylene release rate change trend of each treatment in the later stage of the bottle-plugging is not obviously different from that of the control CK, but the ethylene release rate of each treatment in the later stage of the bottle-plugging is obviously smaller than that of the control CK, and the ethylene release rate is 125 mg.L-1The ethylene release rate of the KYN treatment was lower among the treatments. The addition of 125 mg.L to the base vial insert solution is described-1KYN is mainly used for reducing the release rate of ethylene, so that the effect of delaying the aging of peony cut flowers is achieved.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the technical principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (4)
1. A peony cut flower vase preservative solution is characterized by containing sucrose, sodium hypochlorite, kynurenine and the balance of water.
2. The peony cut vase preservative solution as claimed in claim 1, which comprises 1-5% of sucrose, 0.1-1.0% of sodium hypochlorite and 25-125mg/L of kynurenine, and the balance of water.
3. The peony cut vase preservative solution according to claim 2, which comprises 2% of sucrose, 0.5% of sodium hypochlorite and 125mg/L of kynurenine.
4. A method for prolonging the vase life of a peony vase is characterized by comprising the following steps:
1) putting the peony cut flower into distilled water for re-shearing, and obliquely shearing the base of the flower branch for 45 degrees under water by using special gardening scissors with sharp knife edges;
2) making each cut flower 15-25cm from the base to the top of the stem, and rehydrating for 1-2 h;
3) inserting the rehydrated peony cut flower into the vase preservative solution of any one of claims 1-3.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114600873A (en) * | 2022-01-24 | 2022-06-10 | 南京农业大学 | Summer chrysanthemum fresh cut flower vase preservative solution and method for prolonging cut flower vase life |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102687716A (en) * | 2011-11-25 | 2012-09-26 | 河南科技大学 | Tree peony fresh cutting flower anti-staling agent and preservative solution |
| CN106508972A (en) * | 2016-10-29 | 2017-03-22 | 常州亚环环保科技有限公司 | Preparation method of environment-friendly insecticide |
| CN107047750A (en) * | 2017-06-23 | 2017-08-18 | 徐州市坤元食品有限公司 | A kind of preparation method of strawberry preservative |
| CN111943766A (en) * | 2020-07-31 | 2020-11-17 | 农业农村部环境保护科研监测所 | Cadmium-reducing rice foliar fertilizer and use method thereof |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102687716A (en) * | 2011-11-25 | 2012-09-26 | 河南科技大学 | Tree peony fresh cutting flower anti-staling agent and preservative solution |
| CN106508972A (en) * | 2016-10-29 | 2017-03-22 | 常州亚环环保科技有限公司 | Preparation method of environment-friendly insecticide |
| CN107047750A (en) * | 2017-06-23 | 2017-08-18 | 徐州市坤元食品有限公司 | A kind of preparation method of strawberry preservative |
| CN111943766A (en) * | 2020-07-31 | 2020-11-17 | 农业农村部环境保护科研监测所 | Cadmium-reducing rice foliar fertilizer and use method thereof |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114600873A (en) * | 2022-01-24 | 2022-06-10 | 南京农业大学 | Summer chrysanthemum fresh cut flower vase preservative solution and method for prolonging cut flower vase life |
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