CN101601379A - A kind of antistaling agent for cut-flower - Google Patents
A kind of antistaling agent for cut-flower Download PDFInfo
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- CN101601379A CN101601379A CNA2009100411629A CN200910041162A CN101601379A CN 101601379 A CN101601379 A CN 101601379A CN A2009100411629 A CNA2009100411629 A CN A2009100411629A CN 200910041162 A CN200910041162 A CN 200910041162A CN 101601379 A CN101601379 A CN 101601379A
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Abstract
The invention discloses a kind of antistaling agent for cut-flower, it is by the sugar of 1-3wt%, 0.1-0.3mmol.L
-1Oxinate and 30-120 μ mol.L
-1Resveratrol and/or sodium nitroprussiate form.The present invention provides scientific basis for sodium nitroprussiate in the application aspect the cutflower preservation; The antistaling agent for cut-flower that the pure natural plant extract resveratrol is made has delayed the process of cutting flower withering, and has prolonged a bottle slotting life-span, and has stopped Ag effectively
+Deng the toxic action of nuisance to environment; And the resveratrol source is wide, economical and practical, natural environmental-protective, and safety and stability is used its making antistaling agent for cut-flower and is adopted in the industry of back at the gardening in future, and wide application prospect will be arranged.
Description
Technical field
The invention belongs to the fresh chemically technology, specifically, relate to a kind of antistaling agent for cut-flower.
Background technology
Cut-flower typically refers to the general name of the flower sheared from plant corpus, spray, blade etc., and their are to be also referred to as colored material by material of flower arrangement, are used for flower arrangement or make flower decorations such as bouquet, the gaily decorated basket, wreath.Four traditional big cut-flowers comprise Chinese rose, lily, carnation, sword lily.
Comparatively active always at home and abroad about cut flower withering process and mechanism research for a long time, but cutting flower variety is various, and metabolism difference is big between kind, does not still have effective broad spectrum activity antistaling agent.The preservation method of cut-flower is based on fresh chemically at present, and antistaling agent contains the ethene antagonist of silver nitrate, silver thiosulfate one class mostly, not only cost an arm and a leg, and silver ion is harmful, easily contaminated environment.
Resveratrol has another name called 3,5,4 '-trihydroxy talan, resvertrol, being accredited as at first is a kind of phytoalexin that produces under the unfavorable conditions such as grape, ultraviolet irradiation or mechanical damage, is a kind of biological very strong natural polyphenol class material, and the function that delays plant senesecence is arranged.Up to the present, having found in the plant species surplus 70 has the resveratrol composition, extensively is present in the plants such as polygonaceae, pulse family and Liliaceae, and wherein multiple is common Chinese medicine, as giant knotweed, fallen flowers take root, smilax etc.Studies have shown that in a large number that in recent years resveratrol is the active skull cap components that human health is had clear and definite effect, resveratrol can be competed with ubiquinone in vivo, reduce the formation of composite I II in the respiratory chain, thereby obviously reduce activity in vivo oxygen level, reduce the generation of free radical, show good non-oxidizability.Yet resveratrol is applied to the concrete physiological effect on the plant, and is still very unclear at present.
Nitric oxide (NO) is considered to be in ubiquitous key signal molecule in the plant, has participated in the regulation and control of growth and development processes such as the ripe aging of plant tissue.On the basis of a large amount of gardening products tests, but be suggested may be as a kind of natural plants growth regulator of delayed fruit aging for NO, and its mechanism of action is relevant with the generation of ethene suppressing.Studies show that, in the slotting process of bottle of cut rose flower, the exogenous NO gas donor sodium nitroprusside may reduce the generation of active oxygen radical in the aging course by regulating the activity of lipoxygenase (LOX) in the aging course, thereby suppresses because the ethene that lipid peroxidation causes is synthetic; Also can be by the key enzyme ACC synzyme in the biosynthesis of regulation and control ethene and the activity of acc oxidase, inhibition is owing to the excessive synthetic initiation of ethene cuts flower withering, thus the bottle that prolongs Chinese rose of cut flower is inserted the life-span.
Summary of the invention
The objective of the invention is to overcome deficiency of the prior art, provide a kind of safely, environmental protection ground, the new antistaling agent for cut-flower in cheap ground.
To achieve these goals, the present invention has taked following technical scheme:
A kind of antistaling agent for cut-flower, described antistaling agent is made up of the component of following content:
Sugar 1-3wt%
Oxinate 0.1-0.3mmol.L
-1
Resveratrol and/or sodium nitroprussiate 30-120 μ mol.L
-1
Preferably, described antistaling agent for cut-flower is made up of the component of following content:
Sugar 1-3wt%
Oxinate 0.1-0.3mmol.L
-1
Resveratrol 55-85 μ mol.L
-1
More preferably, described antistaling agent for cut-flower is made up of the component of following weight:
Sucrose 2wt%
Oxine citrate 0.2mmol.L
-1
Preferably, described antistaling agent for cut-flower is made up of the component of following content:
Sugar 1-3wt%
Oxinate 0.1-0.3mmol.L
-1
Sodium nitroprussiate 40-90 μ mol.L
-1
More preferably, described antistaling agent for cut-flower is made up of the component of following weight:
Sucrose 2wt%
Oxine citrate 0.2mmol.L
-1
Sodium nitroprussiate 75 μ mol.L
-1
Sugar in the above-mentioned antistaling agent for cut-flower is sucrose, oxinate (being called for short 8-HQ) is oxine thiosulfuric acid (8-hydroxyquin01ineSultate is called for short 8-HQS) or oxine citrate (8-hydroxyquineCitrade is called for short 8-HQC).
Supply with necessary nutrition, prevent that conduit from stopping up and the biosynthesis of ethene suppressing is 3 necessary factors that prolong cutting flower life, sucrose in the antistaling agent for cut-flower of the present invention provides organic nutrition for cut-flower, thereby and have maintenance organelle structure function to stablize, regulate the effect that Premeabilisation of cells is pressed the promotion moisture absorption; Oxine citrate in the antistaling agent prescription has the effect that suppresses bacterium, yeast, fungi, alleviates microorganism to the fascicular obstruction of scape, is beneficial to the maintenance water balance, and wide-spectrum effective and safety are arranged.In addition, antistaling agent for cut-flower of the present invention can also improve stress resistance of plant, comprises the MDA content and the electrical conductivity that reduce plant.
Utilize the cut-flower of the external source donor sodium nitroprusside processing of NO, its petal relative conductivity and MDA content are significantly less than contrast, and a slotting life-span of the bottle of cut-flower obviously prolongs, for sodium nitroprussiate provides scientific basis in the application aspect the cutflower preservation.
The antistaling agent for cut-flower that the present invention utilizes the pure natural plant extract resveratrol to make comes fresh cut-flowers is adopted post processing, can effectively regulate the cut-flower water balance, improve the water regime in the cut-flower body, alleviate the cytoplasm membrane permeability, reduce film fat levels of peroxide, thereby delay the process of cutting flower withering, prolong bottle and insert the life-span, and stopped Ag effectively
+Deng the toxic action of nuisance to environment; Resveratrol is not only originated wide, and is economical and practical, and natural environmental-protective, safety and stability.Therefore, this efficient physiological regulating control material of application resveratrol is made antistaling agent for cut-flower and is adopted in the industry of back at the gardening in future, and wide application prospect will be arranged.
Description of drawings
Fig. 1 is the cut-flower water balance dynamic change after different antistaling agents are handled;
Fig. 2 is that the cut-flower petal cell membrane permeability after different antistaling agents are handled changes;
Fig. 3 is the cut-flower petal MDA content after different antistaling agents are handled.
Embodiment
Below by test the example and specific embodiment further specify the present invention.
Embodiment 1:
Antistaling agent for cut-flower, it is as follows to fill a prescription:
Sucrose 2wt%
Oxine citrate 0.2mmol.L
-1
Sodium nitroprussiate 75 μ mol.L
-1
Embodiment 2:
Antistaling agent for cut-flower, it is as follows to fill a prescription:
Sucrose 2wt%
Oxine thiosulfuric acid 0.2mmol.L
-1
Embodiment 3:
Antistaling agent for cut-flower, it is as follows to fill a prescription:
Sucrose 2wt%
Oxine citrate 0.2mmol.L
-1
Sodium nitroprussiate 35 μ mol.L
-1
Test example 1:
Utilize the antistaling agent for cut-flower of embodiment 1-3 to carry out following test.
1, test material
Be lily (Lilium) for the trial cut flower, adopt in Guangzhou from changing mountain flower grass base, high hill.Select stalwartness, degree of opening basically identical, sizable spray are as supplying the examination material.With 75% alcohol disinfecting scape, its stem lower end is tiltedly cut before the spray bottle is inserted, keep the about 30cm of spray length, remove most of leaf, only stay 2 compound leaves.The test spray inserts is equipped with in the triangular flask of 200ml antistaling agent, and with the preservative film sealing, divides evaporation to prevent water, places under the room scattering light and tests.Keep 22～25 ℃ of room temperatures, relative moisture 60%-80%.
For determining the suitable concentration of resveratrol and sodium nitroprussiate, use 25,50,100,200 μ mol.L respectively
-1Resveratrol and sodium nitroprussiate, tentatively tested fresh-keeping effect and the toxicity of two kinds of compounds on the lily cut-flower, found that 50 μ mol.L
-1Resveratrol and 100 μ mol.L
-1The sodium nitroprussiate effect is preferable.On this basis, in conjunction with current common cutflower preservation agent prescription, 5 kinds of antistaling agent components and concentration have been selected for test.Concrete prescription sees Table 1, replaces antistaling agent to compare (CK) with distilled water, and each is handled 3 times and repeats.NO donor sodium nitroprusside and resveratrol are produced by Sigma company.
The different antistaling agent prescriptions of table 1
2, test method
From cut-flowers in bottle same day, every day results of regular determination water balance value and fresh weight changing rate.Taking by weighing spray+solution+bottle weight earlier, is fluid losses in weighing during this period of time 2 times with the difference of 2 consecutive weighingses; Same weighing bottle+solution weight calculates water absorption, and the difference of water absorption and fluid loss is the water balance value.Regularly measure flower diameter, get when each processing flower footpath reaches maximum and compare with ruler.The spray fresh weight adopts weight method, spray fresh weight changing rate (%)=[(measuring a day fresh weight-initial fresh weight)/initial fresh weight] * 100%.The slotting life-span judgement of bottle is with petal dehydration wilting, membranousization or brown stain takes place, the petal base portion comes off is sign.Malonaldehyde (MDA) Determination on content adopts the thiobarbituricacid method, and cell leakage is represented with relative conductivity, adopts determination of electrode.Data statistic analysis adopts the DPS data handling system to carry out the multiple comparing check of Duncan ' s.
3, result of the test
(1) comparison of different antistaling agent fresh-keeping effects
Compared with the control, the bottle of the 5 kinds of equal energy of antistaling agent significant prolongation cut-flowers is inserted the life-span, and the increase cut-flower is spent directly, spent length, postpones the slotting time of bottle that flower reaches maximum gauge.Through the cut-flower that antistaling agent is handled, pattern all than the bright-coloured brilliance of control group, spray is stiffening, blue stain is postponed, no elbow phenomenon.Wherein, No. 4 the antistaling agent effect is best, and bottle is inserted the life-span and surpassed one times of time than contrasting prolongation, and maximum spending directly compared according to increasing by 21.6%, reaches the slotting fate of maximum florescence bottle and has also postponed 1d (table 2).No. 3 antistaling agent effects secondly, bottle is inserted the life-span and is surpassed 5d than contrasting to prolong, maximum flower footpath contrast increases by 18.9%, and these two kinds of antistaling agent prescriptions all are better than No. 2 prescriptions that are usually used in the lily cutflower preservation in the market to the fresh-keeping effect of Lilium lily cut-flowers in bottle life-span and ornamental value.And No. 5 antistaling agent prescriptions that are combined into by resveratrol and sodium nitroprussiate are better than No. 2 prescriptions commonly used to the fresh-keeping effect of lily cut-flower, but not as No. 3 and No. 4 obvious.
The different antistaling agents of table 2 are handled back cut-flowers in bottle life-span and flower footpath
Annotate: it is not remarkable that same row same letter is illustrated on 0.05 level difference
(2) different antistaling agents are to the influence of variation of cut-flower fresh weight and water balance value
The cut-flower general trend that fresh weight changes during bottle is inserted all is to rise earlier afterwards to descend, obviously increase of contrast of cut-flower fresh weight after antistaling agent is handled, especially No. 4 antistaling agent effects are the most obvious, reach maximum at the 8d fresh weight, when control group has presented wilting, the fresh weight value is reduced to initial value when following, and the cut-flower under No. 4 antistaling agent is handled still keeps the good state that bursts forth, when having arrived fast wilting, its value still is higher than initial value.
The freshness of cut-flower only just can be kept when water absorption＞fluid loss.As seen from Figure 1, compared with the control, all antistaling agents all have the lily cut-flower effect of water balance time that delays.In the control group behind the lily cut-flowers in bottle water content rise rapidly, negative value just appearred the 6th day water balance, the blue stain phenomenon just appears after flower launches fully in a short time.And the speed of cut-flower suction is mild relatively under each antistaling agent processing, the water balance situation be improved significantly, all be after 9 days, just to reach the water balance point, especially the water balance of No. 3 and No. 4 antistaling agents o'clock just appearance after 10 days, kept petal turgescence preferably, the time retardation that the fresh weight maximum occurs, also contrast is big in the flower footpath, thereby reached the time of delaying senility, the purpose that strengthens cut-flower reward property and prolong viewing period.This shows that antistaling agent is handled, especially antioxidant resveratrol and exogenous NO gas are handled the water regime that can obviously improve in the cut-flower body, delay the wilting process of petal dehydration, prolong the cut-flower shelf life.
(3) different antistaling agents are to the influence of cut-flower cell leakage
As seen from Figure 2, control group petal membrane permeability continue to rise during the lily cut-flowers in bottle, after bottle is inserted the 12nd day, and the petal relative conductivity has surpassed 82%.And under antistaling agent is handled, the petal membrane permeability rises slower, especially membrane permeability is obviously low than other processing in No. 3 and No. 4 antistaling agents, the relative conductivity that bottle is inserted the 12nd day petal in back only is 68.3% and 64.6% of a control group, compare with No. 2 antistaling agents processing of routine, membrane permeability also all significantly is lower than the latter (p＜0.05), make veratryl alcohol and sodium nitroprussiate clear and handle the accumulation that can effectively reduce the petal free radical, help to keep the integrality of petal cell structure, suppress the plasma membrane injury that electrolyte exosmoses and causes.
(4) different antistaling agents are to the influence of cut-flower film fat levels of peroxide
Malonaldehyde (MDA) content with cut flower withering closely related.Owing to cut flower withering in the process, various adverse circumstances are quickened film fat peroxidating chain reactions, and free radical increases, and protective enzyme systems such as SOD are destroyed, then accumulate many harmful peroxidating products, as malonaldehyde etc.Mda content is high more, shows that film fat peroxidating degree is strong more, and plant senescence just rapidly.And MDA itself has tangible toxic action to growth and development of plants and cell division, it and combined with protein cause crosslinked between protein molecule and form cross-linking agent, thereby cell membrane system is deformed, cause that membrane permeability changes, and causes hazard of plant death at last.
The lily cut-flower is inserted in the process at bottle, and MDA content and cell leakage show the trend that similarly progressively rises, and petal MDA content difference is remarkable under the different disposal.Compared with the control, the petal MDA content of handling through antistaling agent all obviously reduces, and the effect that wherein reduces cut-flower film fat levels of peroxide is the most obvious with No. 4 antistaling agents, No. 3 antistaling agents next (Fig. 3).MDA in the plant corpus is produced by the unsaturated fatty acid generation peroxidation of membrane lipids in the film fat, is to weigh the stable important indicator of plasma membrane.MDA content increases, and causes the seepage of film, thereby starts old and feeblely, so MDA content and petal aging are closely related.Experimental result shows, contains the antistaling agent of resveratrol and sodium nitroprussiate, can obviously reduce petal MDA content, thereby helps to delay the aging of flower.
Claims (7)
1. antistaling agent for cut-flower, it is characterized in that: described antistaling agent is made up of the component of following content:
Sucrose 1-3wt%
Oxinate 0.1-0.3mmol.L
-1
Resveratrol and/or sodium nitroprussiate 30-120 μ mol.L
-1
2. antistaling agent for cut-flower according to claim 1 is characterized in that: described antistaling agent is made up of the component of following content:
Sucrose 1-3wt%
Oxinate 0.1-0.3mmol.L
-1
Resveratrol 55-85 μ mol.L
-1
3. antistaling agent for cut-flower according to claim 2 is characterized in that: described antistaling agent is made up of the component of following content:
Sucrose 2wt%
Oxine citrate 0.2mmol.L
-1
Resveratrol 60 μ mol.L
-1
4. antistaling agent for cut-flower according to claim 1 is characterized in that: described antistaling agent is made up of the component of following content:
Sucrose 1-3wt%
Oxinate 0.1-0.3mmol.L
-1
Sodium nitroprussiate 40-90 μ mol.L
-1
5. antistaling agent for cut-flower according to claim 4 is characterized in that: described antistaling agent is made up of the component of following content:
Sucrose 2wt%
Oxine citrate 0.2mmol.L
-1
Sodium nitroprussiate 75 μ mol.L
-1
6. antistaling agent for cut-flower according to claim 1 is characterized in that: described oxinate is oxine thiosulfuric acid or oxine citrate.
7. according to each described antistaling agent for cut-flower of claim 1-6, it is characterized in that: described cut-flower is a lily.
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| CNA2009100411629A CN101601379A (en) | 2009-07-15 | 2009-07-15 | A kind of antistaling agent for cut-flower |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103766475A (en) * | 2014-01-16 | 2014-05-07 | 陕西师范大学 | Application of resveratrol in prevention and control of apple superficial scald and pear melanoderma |
| CN106577639A (en) * | 2016-12-15 | 2017-04-26 | 成都锦汇科技有限公司 | Woody cut flower preservative |
| CN110710391A (en) * | 2019-10-21 | 2020-01-21 | 江苏省农业科学院 | Application of aluminum citrate in color regulation of hydrangea |
-
2009
- 2009-07-15 CN CNA2009100411629A patent/CN101601379A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103766475A (en) * | 2014-01-16 | 2014-05-07 | 陕西师范大学 | Application of resveratrol in prevention and control of apple superficial scald and pear melanoderma |
| CN103766475B (en) * | 2014-01-16 | 2015-12-30 | 陕西师范大学 | The application of resveratrol in control apple superficial scald and pears melanoderma |
| CN106577639A (en) * | 2016-12-15 | 2017-04-26 | 成都锦汇科技有限公司 | Woody cut flower preservative |
| CN110710391A (en) * | 2019-10-21 | 2020-01-21 | 江苏省农业科学院 | Application of aluminum citrate in color regulation of hydrangea |
| CN110710391B (en) * | 2019-10-21 | 2022-04-15 | 江苏省农业科学院 | Application of aluminum citrate in color regulation of hydrangea |
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Application publication date: 20091216 |