CN113748913B

CN113748913B - Pine rejuvenation method

Info

Publication number: CN113748913B
Application number: CN202111196869.4A
Authority: CN
Inventors: 周晓杰; 王开勇; 张纯发; 张冀; 亢亚松; 郭宾武; 郝红旗; 梁瑞
Original assignee: Beijing Jingcai Yanyuan Garden Technology Co ltd; Beijing Jingcai Hongjing Garden Engineering Co ltd
Current assignee: Beijing Jingcai Yanyuan Garden Technology Co ltd; Beijing Jingcai Hongjing Garden Engineering Co ltd
Priority date: 2021-10-14
Filing date: 2021-10-14
Publication date: 2022-09-23
Anticipated expiration: 2041-10-14
Also published as: CN113748913A

Abstract

The application relates to the field of tree planting, and particularly discloses a pine rejuvenation method which comprises the following steps: s1, digging an annular groove: excavating an annular ditch on the ground surrounding the trunk, wherein the distance between the outer diameter of the annular ditch and the trunk is 1/2-2/3 of the vertical projection radius of the crown, the depth of the annular ditch is 15-20cm, and the width of the annular ditch is 5-10 cm; s2, rejuvenation matrix application: filling the annular ditch with rejuvenation matrix, and compacting with original soil, wherein the rejuvenation matrix comprises mycorrhizal fungi. The method for rejuvenating the pine trees has the advantages of improving the soil environment in a nursery, improving the soil fertility, improving the community structure of rhizosphere microorganisms of the pine trees, improving the stress resistance of the pine trees and improving the rejuvenation effect of the pine trees.

Description

Pine rejuvenation method

Technical Field

The application relates to the technical field of tree planting, in particular to a method for rejuvenating pine trees.

Background

The Pinus plant is used as a main tree species of a forest, the application is wide, most of the absorbed roots of seedlings are lost after being transplanted in production practice, the absorption capacity and the transportation capacity of water and nutrient substances are reduced, the nutrient absorption is less, the supply is insufficient, and the physiological function of the trees cannot normally operate.

The soil can supply water and nutrients required by the growth of seedlings, the quality of the soil in the nursery directly influences the growth speed of the seedlings in the nursery and even influences the survival rate of the seedlings, the soil in the nursery is influenced to a certain extent by activities such as artificial management badness, watering, fertilizing and the like in the long-term seedling management and seedling cultivation and maintenance processes, such as soil hardening, soil pollution, salinization and the like, particularly, the soil fertility and the soil quality of the soil in the nursery are reduced after the soil in the nursery is planted for a plurality of years, the production potential of the soil in the nursery is seriously influenced, the root system development of the seedlings is inhibited, the absorption of the seedlings in the nursery to the nutrition, the moisture and the like is limited, and the system stability and the anti-interference capability of the seedlings to the outside are reduced.

Pine transplanted into a nursery has extremely poor rejuvenation capability and slow seedling recovery, influences the overall growth and development of the pine, reduces the stress resistance of the pine, seriously reduces the quality of the pine, increases the greening difficulty, and is difficult to maintain the health and sustainable development of the pine in the nursery.

In view of the above-mentioned related arts, the inventors of the present invention have considered that the rejuvenation ability of pine trees needs to be improved due to insufficient soil fertility and soil quality in a nursery after the pine trees are transplanted into the nursery.

Disclosure of Invention

In order to improve soil fertility in a nursery, improve absorption and utilization of nutrients in soil by pine trees transplanted in the nursery, and further improve the rejuvenation speed of the pine trees, the application provides a method for rejuvenating the pine trees.

In a first aspect, the present application provides a method for rejuvenating pine trees, which adopts the following technical scheme:

a pine rejuvenation method comprises the following steps:

s1, digging an annular groove: excavating an annular ditch on the ground surrounding the trunk, wherein the distance between the outer diameter of the annular ditch and the trunk is 1/2-2/3 of the vertical projection radius of the crown, the depth of the annular ditch is 15-20cm, and the width of the annular ditch is 5-10 cm;

s2, rejuvenation matrix application: filling a rejuvenation substrate into the annular ditch, compacting by using original soil, wherein the rejuvenation substrate comprises mycorrhizal fungi, and the diameter of the pine is marked as H;

when H is less than 10cm, the application amount of the rejuvenation substrate is 0.2-0.3 kg/plant;

when H is more than 10cm and less than or equal to 15cm, the application amount of the rejuvenation substrate is 0.6-1kg per plant;

when H is more than 15cm and less than or equal to 20cm, the application amount of the rejuvenation substrate is 1-1.5 kg/plant;

when H is more than 20cm, the application amount of the rejuvenation substrate is 1.5-2 kg/plant.

By adopting the technical scheme, the mycorrhizal fungi are adopted as rejuvenation matrixes of the pine trees and are applied around the pine trees with different diameters, the application amount is controlled, the mycorrhizal fungi can obviously improve the community structure of rhizosphere microorganisms of the pine trees, the rhizosphere microorganisms existing at the roots of the pine trees can cooperate with the mycorrhizal fungi, the types and the number of the microorganisms are adjusted by changing the microenvironment of rhizosphere soil, the soil environment for the growth of the pine trees is improved, the soil fertility is increased, the stress resistance of the pine trees in a nursery is improved, the morphological development of the pine trees is promoted, the absorption capacity of the pine trees for nutrients and moisture is improved, and the growth of the pine trees is promoted.

Preferably, when the pine is a Chinese pine, the mycorrhizal fungi is a mixture of suillus mucosae, tricholoma giganteum, armillaria tabescens and leuconostoc sphaeroides, and the mass ratio of the suillus mucosae, tricholoma giganteum, armillaria tabescens and leuconostoc sphaeroides is 0.8-1.2:0.8-1.2: 0.8-1.2: 0.8-1.2.

By adopting the technical scheme, boletus viscosus, tricholoma matsutake, armillaria tabescens and leucotrichia coccinea can form mycorrhiza with the Chinese pine, so that the soil enzyme activity and the soil nutrient content are improved, the antioxidant enzyme activity in the Chinese pine is enhanced, and the absorption of the Chinese pine to phosphorus in the environment is remarkably promoted, thereby promoting the growth of the Chinese pine, inhibiting the occurrence of damping-off and improving the disease-resistant effect of the Chinese pine.

Preferably, when the pine is white bark pine, the mycorrhizal fungi is a mixture of the phaeosphaera leucotricha, the suillus luteus and the aeromonas terrestris, and the mass ratio of the mixture of the phaeosphaera leucotricha, the suillus fulva and the aeromonas terrestris is 3.5-4.5:2.8-3.2: 2.8-3.2.

By adopting the technical scheme, the colorful bean puffball, the brown cattle liver and the indigenous cenobium are ectotrophic mycorrhizal fungi of pinus bungeana, hypha of the mycorrhizal fungi can invade the intercellular spaces and cells of all thin-walled tissues including the inner cortex of the root, the thin-walled tissues of the root have functions of assimilation, storage, absorption and the like, the hypha directly invades the tissues, particularly the root medulla part, the close relation between the root and the fungi is improved, the enhancement of the material exchange between the root and the fungi is facilitated, and the hypha can directly convey absorbed nutrients and taxes to the medulla part for the growth and development of plants.

Preferably, the mycorrhizal fungi are prepared by the following method: sterilizing the substrate, uniformly mixing the sterilized substrate and a liquid culture medium according to the weight ratio of 1:2-3 to form a nutrient substrate, cutting mycorrhizal fungi hypha into small pieces, inoculating the small pieces on the nutrient substrate, and culturing for 1 month at 25-28 ℃ in a ventilated and dark manner, wherein the mass ratio of the mycorrhizal fungi hypha to the substrate is 1: 5-10.

By adopting the technical scheme, the sterilized substrate is used as a carrier, the liquid culture medium is used as a nutrient substance, the mycorrhizal fungi rapidly grow and propagate in a dark environment, and the growth vigor is strong, the hypha density is compact, and the activity is high.

Preferably, the substrate is vermiculite.

By adopting the technical scheme, the vermiculite has the advantages of large porosity, large specific surface area and strong adsorbability, can provide more adsorption sites for mycorrhizal fungi, and improves the immobilization efficiency.

Preferably, the substrate further comprises corn flour and chicken manure, and the mass ratio of the vermiculite to the corn flour to the chicken manure is 7:2-2.5: 0.5-1.

By adopting the technical scheme, the chicken manure, the corn flour and the vermiculite are blended to be used as the matrix, the chicken manure and the corn flour can provide a more suitable microenvironment for mycorrhizal fungi, provide nutrient substances such as a carbon source, a nitrogen source, vitamins and the like necessary for the growth of the mycorrhizal fungi, and enable hyphae to continuously grow.

Preferably, the substrate is subjected to a mould method comprising: sterilizing at 80-90 deg.C for 2-3 hr.

By adopting the technical scheme, the substrate is disinfected and sterilized at high temperature, so that bacteria in the substrate can be prevented from influencing the growth of fungi.

Preferably, the rejuvenation substrate also comprises a slow release fertilizer, and the mass ratio of the slow release fertilizer to the mycorrhizal fungi is 1-1.5: 1.

By adopting the technical scheme, pine growth is a long-term and slow process, common fertilizer is applied during transplanting, the fertilizer efficiency time is short, the requirement of pine in the growth period on nutrients cannot be met, the fertilizer needs to be applied repeatedly, the cost is increased, and the instant and long-acting fertility can be achieved and the nutrient utilization rate is improved by using the slow release fertilizer.

The slow release fertilizer is prepared by the following method: (1) uniformly stirring 3-5 parts by weight of mesoporous silica and 1-2 parts by weight of biochar, extruding, granulating, sterilizing to obtain carrier particles, and fermenting the carrier particles and the compound fertilizer at the temperature of 18-30 ℃ for 3-5 days according to the mass ratio of 2-3:1 to obtain a fermentation material;

(2) carrying out superfine grinding on 1-2 parts by weight of activated humic acid, uniformly mixing with 1.5-2.5 parts by weight of water to prepare a binder, uniformly mixing the binder and a fermentation material according to the mass ratio of 0.5-1:1, and carrying out extrusion granulation to prepare bacterial fertilizer particles;

(3) mixing 1-2 parts by weight of distiller's grain organic fertilizer and 2-4 parts by weight of pyroligneous diluted by 50-100 times, sealing and standing for 8-10 days, stirring once every 2-3 days, drying, concentrating and drying, and preparing into particles;

(4) dissolving 1-2 parts by weight of polyvinyl alcohol, adding 0.8-1.2 parts by weight of earthworm fertilizer, uniformly mixing to form coating liquid, spraying the coating liquid on the particles obtained in the step (3), drying, mixing with the bacterial fertilizer particles obtained in the step (2), and preparing the slow release fertilizer.

By adopting the technical scheme, the compound fertilizer is mixed with carrier particles formed by mesoporous silicon dioxide and biochar, and the mesoporous silicon dioxide and the biochar have larger specific surface area and porosity, so that the compound fertilizer can be conveniently loaded on the surface of the mesoporous silicon dioxide and the biochar; the activated humic acid has small grain diameter after being subjected to superfine grinding, has a bonding effect, can bond carrier particles loaded with the compound fertilizer together, and can reduce the molecular weight of the activated humic acid, increase functional groups and water solubility, promote rooting beams and enhance stress resistance.

Because the optimum pH value for pine growth is 6.5-7.5, and the pH value is slightly acidic or neutral, the diluted pyroligneous liquor is used for soaking the vinasse organic fertilizer in the application to acidify the vinasse organic fertilizer, the diluted pyroligneous liquor is added into soil, the acidity of the soil can be reduced, the acidity of the soil is suitable for pine growth, the vinasse organic fertilizer contains a large amount of compound floras such as lactic acid bacteria and saccharomycetes, and the pyroligneous liquor diluted by 50-100 times does not influence the growth and reproduction of the floras and can not cause soil acidification.

And finally, spraying a polyvinyl alcohol solution containing the earthworm fertilizer on the acidified vinasse organic fertilizer, wherein polyvinyl alcohol forms a coating film on the acidified vinasse organic fertilizer, the earthworm fertilizer is adhered to the coating film, and the vinasse organic fertilizer coated inside is gradually released along with the degradation and slow release effects of the polyvinyl alcohol coating film in soil to acidify and improve the soil and continuously increase the soil fertility.

Preferably, the compound fertilizer is selected from one or more of ferrous sulfate, ammonium phosphate and phosphogypsum.

By adopting the technical scheme, the ferrous sulfate can supplement iron elements in plants and promote the absorption of the iron elements to nitrogen and phosphorus elements, so that the decomposition of organic matters in soil is accelerated, and the ferrous sulfate is acidic, so that the soil is acidic and is more suitable for pine growth; the phosphogypsum contains abundant soluble calcium, sulfur, phosphorus and trace elements, can improve soil hardening, and promotes nitrogen absorption of pine trees.

In summary, the present application has the following beneficial effects:

1. the method adopts the steps that the annular groove is dug on the ground surrounding the trunk, the mycorrhizal fungi are buried in the annular groove to serve as the rejuvenation matrix to rejuvenate the pine transplanted into the nursery, the mycorrhizal fungi can supplement the mycorrhizal deficiency of the pine, the community structure and the micro-ecological environment enzyme of the rhizosphere microorganisms of the pine are improved, the microenvironment of rhizosphere soil is changed to adjust the variety and the number of the rhizosphere microorganisms, the nutrient and water absorption capacity of the nursery stock is improved, the growth of the pine is promoted, the content of nitrogen and phosphorus in the regenerated leaves of the pine is improved, and the soil environment is improved, so that the pine transplanted into the nursery is rejuvenated quickly.

2. Preferably adopt suillus mucor, tricholoma palmatum, leucorhizopus luteus and leucotrichum mycorrhiza to rejuvenate the white bark pine as the rejuvenation matrix in this application, use colourful bean puffball, soil living aerobium and suillus luteus as the rejuvenation matrix to rejuvenate the white bark pine, because the root system development condition of different pine is different, and different mycorrhizal fungi show that the adaptation time is different, adopt the mycorrhizal fungi to rejuvenate white bark pine and white bark pine in this application, can effectively promote the growth of white bark pine and white bark pine.

3. According to the application, the slow release fertilizer prepared from mesoporous silica, biochar, earthworm fee, vinasse organic fertilizer and the like is preferably added into the rejuvenation matrix, and is slowly released in soil, so that the content of nutrient elements in the soil is improved, the air permeability of the soil is increased, and the rejuvenation effect of pine trees is further improved.

Detailed Description

Preparation example of mycorrhizal fungi

Preparation example 1: sterilizing the substrate, mixing with liquid culture medium at a weight ratio of 1:2-3 to form a nutritional substrate, cutting mycorrhizal fungi mycelium into small pieces, and inoculating on the nutritional substratePlacing the culture medium in a sterile bag for ventilation and light-proof culture at 25 ℃ for 1 month, wherein the mass ratio of mycorrhizal fungi hypha to a substrate is 1:5, the substrate is vermiculite, and the sterilization method of the substrate is as follows: sterilizing vermiculite at 80 deg.C for 3h, and liquid culture medium comprises the following components: the liquid culture medium comprises the following components: 20g/L calcium chloride, 0.15g/L magnesium sulfate, 0.025g/L sodium chloride, 0.03g/L ferric chloride, 0.5g/L monopotassium phosphate, 2.5g/L ammonium tartrate, 5g/L glucose, 2.5g/L wort, 0.2g/L citric acid, 0.025g/L vitamin B1 and 1L distilled water, wherein mycorrhizal fungi hypha are cultured by the following method: (1) extracting a bacterium block: taking fresh fungus fruiting bodies which are not opened, have no damage, no plant diseases and insect pests and no pollution, and carrying out tissue separation by adopting the following method to obtain fungus blocks: cutting off overlong stipe, sterilizing in an inoculation box, scrubbing pileus and stipe with 75% alcohol, longitudinally cutting off from the middle of stipe with sterile knife, and picking 2cm at the junction of pileus and stipe ² The mycorrhizal fungi is suillus annuus;

(2) inoculating and activating strains; the bacterial block is inoculated on MS culture medium and cultured for 24h at 25 ℃.

Preparation examples 2 to 7: the difference from preparation example 1 is that the mycorrhizal fungi were selected as shown in Table 1.

Examples 8 to 14: the difference from preparation example 1 is that the selection of the substrate is shown in Table 1.

TABLE 1 selection of substrate and mycorrhizal fungi in preparations 1-14

Preparation example	Substrate	Mycorrhizal fungi
			Preparation example 1	Vermiculite	Boletus viscosus
Preparation example 2	Vermiculite	Tricholoma matsutake (lto et lmai) Singer
			Preparation example 3	Vermiculite	Phyllostachys nervosa (Fr.) Kuntze
Preparation example 4	Vermiculite	Leucosceptrum coccobagrum
			Preparation example 5	Vermiculite	Colorful bean puffball
Preparation example 6	Vermiculite	Boletus luteus (Fr.) pers
			Preparation example 7	Vermiculite	Aerial cenosphaeria
Preparation example 8	The mass ratio of the vermiculite to the corn flour to the chicken manure is 7:2:1	Boletus viscosus
			Preparation example 9	The mass ratio of the vermiculite to the corn flour to the chicken manure is 7:2:1	Tricholoma matsutake (lto et lmai) Singer
Preparation example 10	The mass ratio of the vermiculite to the corn flour to the chicken manure is 7:2:1	Phyllostachys nervosa (Fr.) Kuntze
			Preparation example 11	The mass ratio of vermiculite to corn flour to chicken manure is 7:2:1	Leucosceptrum coccobagrum
Preparation example 12	The mass ratio of the vermiculite to the corn flour to the chicken manure is 7:2:1	Colorful bean puffball
			Preparation example 13	The mass ratio of the vermiculite to the corn flour to the chicken manure is 7:2:1	Boletus luteus (Fr.) pers
Preparation example 14	The mass ratio of the vermiculite to the corn flour to the chicken manure is 7:2:1	Aerial cenosphaeria

Preparation example of Slow Release Fertilizer

Preparation examples 15-19 mesoporous silicas selected from the group consisting of XFF29, model 102971, 400nm in diameter; the biochar is selected from the arisaema source environment-friendly materials Co, and the model is xy-002562; the activated humic acid is selected from Houma Shengjiayou Biotech development Limited company; the vinasse organic fertilizer is selected from Guangdong Hua ecological science and technology limited company with the product number of 8; the wood vinegar is selected from chemical industry Limited of Jianjun in Jinan, and the product number is 001; the polyvinyl alcohol is selected from Zhengzhou Feiteng chemical industry Co., Ltd, and the cargo number is 24-88; the earthworm fertilizer is selected from Sakyamuanning Probiotics science and technology ltd, with the product number x 34.

Preparation example 15: (1) uniformly stirring 300g of mesoporous silica and 100g of biochar, extruding, granulating, sterilizing to obtain carrier particles, and fermenting the carrier particles and a compound fertilizer at a mass ratio of 2:1 at 18 ℃ for 3 days to obtain a fermented material, wherein the compound fertilizer is ferrous sulfate;

(2) carrying out superfine grinding on 100g of activated humic acid, uniformly mixing with 150g of water to prepare a binder, uniformly mixing the binder and a fermentation material according to a mass ratio of 0.5:1, and carrying out extrusion granulation to prepare bacterial fertilizer particles;

(3) mixing 100g of distiller's grain organic fertilizer and 200g of pyroligneous diluted by 50 times, sealing and standing for 8 days, stirring once every 2 days, drying, concentrating and drying, and preparing into granules;

(4) dissolving 100g of polyvinyl alcohol, adding 80g of earthworm fertilizer, uniformly mixing to form a coating solution, spraying the coating solution on the particles obtained in the step (3), drying, mixing with the bacterial fertilizer particles obtained in the step (2), and preparing the slow release fertilizer.

Preparation example 16: (1) uniformly stirring 500g of mesoporous silica and 200g of biochar, extruding, granulating, sterilizing to obtain carrier particles, and fermenting the carrier particles and a compound fertilizer at a mass ratio of 3:1 at 30 ℃ for 5 days to obtain a fermented material, wherein the compound fertilizer is ferrous sulfate;

(2) carrying out superfine grinding on 200g of activated humic acid, uniformly mixing with 250g of water to prepare a binder, uniformly mixing the binder and a fermentation material according to a mass ratio of 1:1, and carrying out extrusion granulation to prepare bacterial fertilizer particles;

(3) mixing 200g of distiller's grain organic fertilizer and 400g of wood vinegar diluted by 100 times, sealing, standing for 10 days, stirring once every 3 days, drying, concentrating, drying, and granulating;

(4) dissolving 200g of polyvinyl alcohol, adding 120g of earthworm fertilizer, uniformly mixing to form a coating solution, spraying the coating solution on the particles obtained in the step (3), drying, mixing with the bacterial fertilizer particles obtained in the step (2), and preparing the slow release fertilizer.

Preparation example 17: the difference from preparation example 16 is that no compound fertilizer was added.

Preparation example 18: the difference from preparation example 16 is that, without performing step (3), a coating solution made of polyvinyl alcohol and an earthworm fertilizer was sprayed on the bacterial manure particles obtained in step (2).

Preparation example 19: the difference from preparation example 16 is that no earthworm fertilizer is added in step (4).

Examples

In the case of pine rejuvenation, the rejuvenation base was applied in the following amounts: when the H is less than or equal to 10cm, the application amount of the rejuvenation substrate is 0.2-0.3kg per plant; when H is more than 10cm and less than or equal to 15cm, the application amount of the rejuvenation substrate is 0.6-1kg per plant; when H is more than 15cm and less than or equal to 20cm, the application amount of the rejuvenation substrate is 1-1.5 kg/plant; when H > 20cm, the rejuvenating base is applied in an amount of 1.5-2kg per piece, in the examples pine trees with a diameter of less than 10 cm.

Example 1: a pine rejuvenation method comprises the following steps:

s1, digging an annular groove: digging an annular ditch on the ground surrounding a 9 cm-diameter Chinese pine trunk, wherein the distance between the outer diameter of an annular head and the trunk is 2/3 of the vertical projection radius of a crown, the depth of the annular ditch is 20cm, and the width of the annular ditch is 10 cm;

s2, rejuvenation matrix application: the annular ditch is filled with 0.2kg of rejuvenation substrate, the rejuvenation substrate is mycorrhizal fungi, the mycorrhizal fungi are prepared by mixing the boletus mucosae prepared in the preparation example 1, the tricholoma lobayense heim prepared in the preparation example 2, the armillaria tabescens prepared in the preparation example 3 and the leucotrichia sphenanthera prepared in the preparation example 4, and the mass ratio of the boletus mucosae to the tricholoma lobayense heim, the armillaria tabescens and the leucotrichia sphenanthera is 1:1:1: 1.

Example 2: a pine rejuvenation method is different from that in example 1 in that mycorrhizal fungi are prepared by mixing Boletus mucosae prepared in preparation example 8, Tricholoma palmatum prepared in preparation example 9, Tricholoma flavescens in preparation example 10 and Leucosceps sphaeroides prepared in preparation example 11 in a mass ratio of 1:1:1: 1.

Example 3: a pine rejuvenation method which differs from example 1 in that the mycorrhizal fungus is xanthorrhizal agriculmis.

Example 4: a pine rejuvenation method which differs from example 1 in that the mycorrhizal fungus is leucotrichum coccolii.

Example 5: a pine rejuvenation method differing from example 1 in that mycorrhizal fungi were prepared by mixing Leuconostoc communis prepared from preparation example 11 and Tricholoma palmatum prepared from preparation example 9 in a mass ratio of 1: 1.

Example 6: a pine rejuvenation method differs from example 1 in that mycorrhizal fungi are prepared by mixing the Boletus mucosae prepared in preparation example 8 and the Phyllotrex superficialis prepared in preparation example 10 in a mass ratio of 1: 1.

Example 7: a pine rejuvenation method differs from example 1 in that the mycorrhizal fungus is Boletus mucosae.

Example 8: a pine rejuvenation method is different from that in example 2 in that a rejuvenation substrate is a slow-release fertilizer and mycorrhizal fungi in a mass ratio of 1:1, and the slow-release fertilizer is prepared according to preparation example 15.

Example 9: a pine rejuvenation method is different from that in example 2 in that a rejuvenation substrate is a slow-release fertilizer and mycorrhizal fungi in a mass ratio of 1.5:1, and the slow-release fertilizer is prepared in preparation example 16.

Example 10: a pine rejuvenation method, which is different from the embodiment 2 in that a rejuvenation substrate is a slow-release fertilizer and mycorrhizal fungi in a mass ratio of 1:1, and the slow-release fertilizer is prepared by the preparation example 17.

Example 11: a pine rejuvenation method, which is different from the embodiment 2 in that a rejuvenation substrate is a slow-release fertilizer and mycorrhizal fungi in a mass ratio of 1:1, and the slow-release fertilizer is prepared by the preparation example 18.

Example 12: a pine rejuvenation method is different from that in example 2 in that a rejuvenation matrix is a slow release fertilizer and mycorrhizal fungi in a mass ratio of 1:1, and the slow release fertilizer is prepared according to preparation example 19.

Example 13: a pine rejuvenation method comprises the following steps:

s1, digging an annular groove: excavating an annular ditch on the ground surrounding a bark pine trunk with the diameter of 11cm, wherein the distance between the outer diameter of an annular head and the trunk is 1/2 of the vertical projection radius of a crown, the depth of the annular ditch is 15cm, and the width of the annular ditch is 5 cm;

s2, rejuvenation matrix application: the annular ditch is filled with 0.6kg of rejuvenation substrate, the rejuvenation substrate is compacted by raw soil, the rejuvenation substrate is mycorrhizal fungi, the mycorrhizal fungi are prepared by mixing the colorful bean puffball prepared in the preparation example 5, the suillus luteus prepared in the preparation example 6 and the aeromonas sobria prepared in the preparation example 7, and the mass ratio of the colorful bean puffball, the suillus luteus and the aeromonas sobria is 4:3: 3.

Example 14: a pine rejuvenation method is different from that in example 12 in that mycorrhizal fungi are prepared by mixing Phaseolus versicolor prepared in preparation example 12, Boletus luteus prepared in preparation example 13 and Boletus terrestris prepared in preparation example 14, and the mass ratio of the Phaseolus versicolor, the Boletus luteus and the Boletus terrestris is 4:3: 3.

Example 15: a pine rejuvenation method which differs from example 13 in that the mycorrhizal fungus is lasiocarpa.

Example 16: a pine rejuvenation method which differs from example 13 in that the mycorrhizal fungi are indigenous airborne microorganisms.

Example 17: a pine rejuvenation method, which is different from the embodiment 13 in that mycorrhizal fungi comprise suillus luteus and bolete terrestris in a mass ratio of 1: 1.

Example 18: a pine rejuvenation method differs from that of example 13 in that the mycorrhizal fungi are Boletus luteus and Pistis versicolor in a mass ratio of 3: 4.

Example 19: a pine rejuvenation method which differs from example 13 in that the mycorrhizal fungus is Boletus mucosae.

Example 20: a pine rejuvenation method, which is different from example 14 in that a rejuvenation substrate is a slow-release fertilizer and mycorrhizal fungi in a mass ratio of 1:1, and the slow-release fertilizer is prepared according to preparation example 15.

Example 21: a pine rejuvenation method, which is different from example 14 in that a rejuvenation substrate is a slow-release fertilizer and mycorrhizal fungi in a mass ratio of 1.5:1, and the slow-release fertilizer is prepared in preparation example 16.

Example 22: a pine rejuvenation method, which is different from example 14 in that a rejuvenation substrate is a slow-release fertilizer and mycorrhizal fungi in a mass ratio of 1:1, and the slow-release fertilizer is prepared according to preparation example 17.

Example 23: a pine rejuvenation method is different from that in example 14 in that a rejuvenation matrix is a slow release fertilizer and mycorrhizal fungi in a mass ratio of 1:1, and the slow release fertilizer is prepared in preparation example 18.

Example 24: a pine rejuvenation method, which is different from example 14 in that a rejuvenation substrate is a slow-release fertilizer and mycorrhizal fungi in a mass ratio of 1:1, and the slow-release fertilizer is prepared according to preparation example 19.

Comparative example

Comparative example 1: a pine rejuvenation method differing from example 2 in that the amount of rejuvenating base applied was 0.1 kg.

Comparative example 2: a pine rejuvenation method is different from that in example 2 in that water is added for pouring mycorrhizal fungi, and the mass ratio of the mycorrhizal fungi to the water is 1: 25.

Comparative example 3: a pine rejuvenation method which differs from example 14 in that the rejuvenation base was applied in an amount of 0.5 kg.

Comparative example 4: a pine rejuvenation method which differs from example 14 in that the mycorrhizal fungi is irrigated with water in a mass ratio of mycorrhizal fungi to water of 1: 25.

Performance test

Detection of rejuvenation effect of Chinese pine

Selecting Chinese pine with trunk of 9cm (selecting nursery stock growing for more than 2 years in Beijing city), equally dividing into 15 groups, 10 in each group, transplanting into the nursery, wherein the distance between two adjacent trees is 4m, rejuvenating 1-14 groups of Chinese pine according to the method in the embodiment 1-12 and the comparative example 1-2, taking the 15 group of Chinese pine without any treatment as a control group, keeping the culture conditions such as watering amount, illumination, ventilation, temperature and the like consistent, detecting the content of nutrient elements in soil with depth of 10-30cm in the nursery before and after rejuvenating the Chinese pine, detecting the ground diameter, height, new branch length and new needle leaf length of the Chinese pine before and after 24 months of growth, taking the average value of the measurement results of 10 Chinese pine in each group, respectively recording the content of nutrient elements in soil in the nursery before and after rejuvenating in tables 2 and 3, the results of the growth measurements before and after rejuvenation of the pinus tabulaeformis are recorded in table 4.

The method for detecting the content of the nutrient elements in the soil comprises the following steps:

(1) organic matter: according to NT/Y1121.6-2006, soil detection part 6: detecting soil organic matter;

(2) available phosphorus: according to NY/T1121.7-2014, part 7 of soil testing: detecting the soil available phosphorus determination;

(3) quick-acting potassium: detecting according to NY/T889-2004 'determination of soil quick-acting potassium and slow-acting potassium content';

(4) hydrolyzable nitrogen: detection was carried out according to NY/T1228-2015 "determination of forest soil nitrogen".

The method for detecting the growth conditions of the Chinese pine before and after rejuvenation comprises the following steps:

1. ground diameter: measuring the diameter of the Chinese pine at a position 0.1m away from the ground by using a vernier caliper to be accurate to 0.01 cm;

2. tree height: measuring from the ground diameter to the base of the terminal bud, and measuring by using a sliding staff to the accuracy of 1 cm;

3. length of the new branch: measuring the length of a new branch by using a ruler, measuring each pinus tabulaeformis 10 times, and averaging to obtain the value which is accurate to 1 cm;

4. the length of the new needle leaf: the length of each needle bundle was measured with a ruler, 10 times per pine, and the average was taken to 0.1 cm.

TABLE 2 nutrient content of soil in nursery before rejuvenation

TABLE 3 soil nutrient content after rejuvenation for each example and comparative example

Before rejuvenation, the contents of organic matters, available phosphorus, quick-acting potassium and hydrolyzable nitrogen in soil at a position of 10-30cm in a nursery garden are low; in example 1, the rejuvenation substrate prepared by mixing the suillus viscosus prepared in preparation example 1, the tricholoma giganteum prepared in preparation example 2, the armillaria tabescens prepared in preparation example 3 and the leucotrichum coccineum prepared in preparation example 4 was used, the mass ratio of the suillus viscosus, the tricholoma giganteum, the armillaria tabescens and the leucotrichum coccineum was 1:1:1, and the oil pine with a diameter of less than 10cm was rejuvenated by burying a circular groove, and the growth rates of organic matter, available phosphorus, available potassium and hydrolyzable nitrogen in the soil were 95.6%, 36.1%, 20.2% and 7.6% respectively 24 months after rejuvenation, thereby improving the soil structure.

The difference between example 2 and example 1 is that the rejuvenation substrate prepared by mixing together suillus viscosus prepared in preparation example 8, tricholoma citrinum prepared in preparation example 9, armillaria tabescens prepared in preparation example 10, and leucotrichum coccineum prepared in preparation example 11 was found to increase the content of organic matter and other components in the soil after rejuvenation compared to example 1, indicating that the activity of mycorrhizal fungi was enhanced by using corn flour, chicken manure, and vermiculite as the substrate.

In example 3, the use of Phyllotreta lutescens alone, and in example 6, the use of Boletus mucosus and Phyllotrex lutescens, compared to example 1, showed that the growth of the soil organic matter and other components after rejuvenation in example 3 was less than that in example 6, and demonstrated that the use of Boletus mucosus and Phyllotrex lutescens could increase the content of nutrient elements in the soil and improve the soil structure.

In example 4 using Leucopharomyces globularis alone, and in example 5 using Leucopharomyces globularis and Tricholoma coioides, the growth rates of organic matter, available phosphorus, available potassium and hydrolyzable nitrogen were 39.0%, 8.4, 6.5% and 2.0% respectively, in example 4, the growth rates of organic matter, available phosphorus, available potassium and hydrolyzable nitrogen were 74.6%, 15.5%, 10.2% and 5.7% respectively, and the improvement effect on nursery soil was superior to that of example 3 in example 5.

In example 7, the use of boletus mucosae reduced the effect of improving the soil structure as compared with example 1, and the effect was inferior to that of the P.basophilus in example 3 and the Leuconostoc sphaericus in example 4.

In examples 8 and 9, the slow release fertilizers prepared in preparation examples 15 and 16 were added to the rejuvenation base materials, respectively, and the organic matter, available phosphorus, quick-acting potassium and hydrolyzable nitrogen in the nursery soil in example 8 were increased by 30g/kg, 15.1mg/kg, 48.2mg/kg and 37.94mg/kg, respectively, compared with those before rejuvenation, and the increase rate was higher than that in example 2, and the effect of improving the soil structure was further improved.

In example 10, example 11 and example 12, when the slow release fertilizers prepared in preparation examples 17, 18 and 19 were used, respectively, the content of organic matter and other substances in the soil in the nursery was reduced after rejuvenation as compared with example 8.

Compared with example 1, the contents of various nutrient elements in the soil after rejuvenation in comparative example 1 and comparative example 2 are reduced by applying only 0.1kg of rejuvenation substrate in comparative example 1 and applying the rejuvenation substrate in a manner of mixing and irrigating with water in comparative example 2.

TABLE 4 measurement results before and after rejuvenation of Pinus tabulaeformis

TABLE 4

As can be seen from the data in examples 1 to 12 and table 4, in example 1, the leucotrichum globosum, tricholoma giganteum, boletus viscosus and ventral xanthorrhizal fungi in the mass ratio of 1:1:1:1 were used as rejuvenating substrates, and the rejuvenation was performed on the pinus tabulaeformis 8cm in diameter by burying the annular groove, and after 24 months of growth, the ground diameter growth rate of the pinus tabulaeformis was 19.9%, the tree height growth rate of the pinus tabulaeformis was more than 30.2%, the growth rate of the new shoot length and the needle leaf length was more than 30%, which indicates that the growth of the root system and the needle cluster of the pinus tabulaeformis can be effectively promoted and the rejuvenation effect after the transplantation of the pinus tabulaeformis facilitated by burying the annular groove with the leucotrichum globosum, tricholoma giganteum phaeodorum palmiformidis, boletum mucor and ventral xanthorrhiza aea in the mass ratio of 1:1:1: 1.

In example 2, corn starch, chicken manure and vermiculite are used as culture carriers of mycorrhizal fungi, and prepared leucotrichia spherulata, tricholoma matsutake, boletus viscosus and armillaria tabescens are used as rejuvenation substrates, after rejuvenation, the ground diameter growth rate of Chinese pine is 21.6%, and in addition, the growth rate of detection items such as the length of a new branch is obviously greater than that of example 1, which shows that the corn starch and the chicken manure can further improve the soil improvement effect of the mycorrhizal fungi.

In example 3, the growth rate of the ground diameter of the Chinese pine is 15.3% by using only the armillaria tabescens, while in example 6, the growth rate of the ground diameter of the Chinese pine is 18.3% by using the armillaria tabescens and the bolete suis, and in example 3, the growth effect of the tree height, the length of new branches and the length of new needles is not as good as that of example 6, while in example 6, the growth tendency of the Chinese pine is not as good as that of examples 1 and 2, which shows that the armillaria tabescens and the bolete suis can cooperate to improve the growth of the root system, the ground diameter and the tree height of the Chinese pine and promote the growth of new branches.

In example 4, only Leuconostoc sphaericus was used as the mycorrhizal fungus, the growth rate of the ground diameter of the pine was 15.1%, while in example 5 in which Leuconostoc sphaericus and Tricholoma palmatum were added, the growth rate of the ground diameter of the pine was 17.5%, indicating that Tricholoma palmatum and Leuconostoc sphaericus could cooperate to promote the growth of the trunk of the pine.

In example 7, boletus caerulea was used, and after rejuvenation, the growth rate of the ground diameter of the pinus tabulaeformis was 13.1%, which was inferior to that of example 1, and inferior to that of examples 3 and 4.

In examples 8 and 9, compared with example 2, when the slow release fertilizers prepared in preparation examples 15 and 16 were added to the rejuvenating substrate, the diameter of the Chinese pine, the height of the tree, the length of the new branch, and the like were all increased, the increase rate of the diameter of the Chinese pine in example 8 was 28.1%, and the increase rate of the diameter of the Chinese pine in example 2 was 21.6%, which indicates that the rejuvenation effect of the Chinese pine in example 8 was further enhanced.

In examples 10 to 12, the slow release fertilizers prepared in preparation examples 17, 18 and 19 were used, respectively, and the ground diameter and the like of the pinus tabulaeformis were reduced as compared with example 8, which indicates that the slow release fertilizers prepared in preparation examples 17 to 19 had inferior rejuvenation effects on the pinus tabulaeformis to preparation example 15.

In comparative example 1, the amount of rejuvenating base applied to 8cm diameter pine was only 0.1kg, and after rejuvenation, the growth condition of the pine was not good, and the height of the tree, the diameter of the ground, etc. were not good as in example 1.

In comparative example 2, the rejuvenation substrate is applied to the root system of the Chinese pine in a manner of mixing with water and irrigating, and after rejuvenation, the root system of the Chinese pine develops poorly and the growth force is inferior to that of example 1.

In the control group, the Chinese pine is not subjected to any rejuvenation treatment, the Chinese pine grows slowly, the needle leaves are sparse, the color of the needle leaves is light green, and the growth vigor is obviously inferior to that of the rejuvenation of the Chinese pine.

Secondly, detecting the rejuvenation effect of the white bark pine:

selecting white bark pines with the trunk diameter of 11cm (selecting seedlings growing in a nursery garden of Beijing city for more than 2 years), averagely dividing into 15 groups, 10 in each group, transplanting the 10 groups into the nursery garden, keeping the distance between two adjacent trees to be 4 meters, rejuvenating the 1 st to 14 th groups of white bark pines according to the methods in the examples 1 to 12 and the comparative examples 1 to 2, taking the 15 th group of white bark pines without any treatment as a control group, keeping the culture conditions of watering quantity, illumination, ventilation, temperature and the like consistent, detecting the content of nutrient elements in soil with the depth of 10-30cm in the nursery garden before and after rejuvenating the white bark pines, detecting the ground diameter, height, new branch length and new needle leaf length of the white bark pines before and after 24 months of growth, taking the average value of the measurement results of the 10 white bark pines in each group, respectively recording the content of the nutrient elements in the soil in the nursery garden before and after rejuvenation in tables 5 and 6, the measurement results of the growth before and after rejuvenation of the lacebark pine are recorded in table 7.

② a soil nutrient element content detection method:

③ the method for detecting the growth condition before and after rejuvenation of the white bark pine is as follows:

1. land diameter: measuring the diameter of the white bark pine at a position 0.1m away from the ground by using a vernier caliper to be accurate to 0.01 cm;

3. length of the new branch: measuring the length of a new branch by using a ruler, measuring each bark pine for 10 times, and averaging to obtain an average value which is accurate to 1 cm;

4. the length of the newly born needle leaf: measuring the length of each needle bundle by using a ruler, measuring each white bark pine for 10 times, and taking an average value to be accurate to 0.1 cm.

TABLE 5 nutrient content of soil in nursery before rejuvenation

Depth of sample	Organic matter g/kg	Effective phosphorus mg/kg	Quick-acting potassium mg/kg	Hydrolyzable nitrogen mg/kg
					10-30cm	24.4	17.8	125.7	80.26

TABLE 6 Nutrition element content of soil in Nursery after rejuvenation

Before rejuvenation, the contents of organic matters, available phosphorus, quick-acting potassium and hydrolyzable nitrogen in soil at a position of 10-30cm in a nursery garden are low; in example 13, a rejuvenating substrate prepared by mixing the colored bean puffball prepared in preparation example 5, the suillus luteus prepared in preparation example 6 and the aeromonas campestris prepared in preparation example 7 was used, the mass ratio of the colored bean puffball, the suillus luteus and the aeromonas campestris was 4:3:3, and the oil pine 11cm in diameter was rejuvenated by burying a circular trench, and the growth rates of organic matter, available phosphorus, available potassium and hydrolyzable nitrogen in the soil were 85.2%, 37.1%, 21.2% and 12.6% respectively 24 months after rejuvenation, so that the soil structure was improved.

Example 14 is different from example 13 in that the rejuvenation substrate prepared by mixing the colored puffball prepared in preparation example 12, the suillus luteus prepared in preparation example 13 and the aeromonas campestris prepared in preparation example 14 further increased the contents of organic matters and other components in the soil after rejuvenation compared with example 13, indicating that the activity of mycorrhizal fungi was enhanced by using corn flour, chicken manure and vermiculite as the substrate.

In example 15, the chromous bean puffball alone is used, and in example 18, the chromous bean puffball and the suillus luteus are used, and the content of nutrient components such as organic matters in the soil rejuvenated in example 15 is lower than that in example 18, which shows that the content of nutrient elements in the soil can be increased and the soil structure can be improved by the suillus luteus and the rhizoctonia flavescens.

In example 16, airborne bolete alone, and in example 17, airborne bolete and suillus luteus were used, and the content of organic matter and other components in example 16 was lower than that in example 17, which indicates that airborne bolete and suis luteus have a better effect of improving the soil structure.

In example 19, the effect of improvement of the soil structure was reduced compared to example 13 by using boletus lanuginosus, and the effect was inferior to that of the colored leguminous puffball in example 15 and that of the airborne microorganism in example 16.

In examples 20 and 21, the slow release fertilizers prepared in preparation examples 15 and 16 were added to the rejuvenation base materials, respectively, and in example 20, the organic matter, available phosphorus, quick-acting potassium and hydrolyzable nitrogen in the nursery soil were increased by 28.4g/kg, 14.6mg/kg, 50.1mg/kg and 38.34mg/kg compared with those before rejuvenation, and the growth values of the respective nutrient elements were greater than those in example 13, thereby further enhancing the effect of improving the soil structure.

In example 22, example 23 and example 24, the slow release fertilizers prepared in preparation examples 17, 18 and 19 were used, respectively, and the content of organic matters and the like in the soil in the nursery was reduced after rejuvenation as compared with example 20.

In comparative example 3, only 0.5kg of rejuvenation substrate is applied to the white bark pine, in comparative example 4, the rejuvenation substrate is applied in a mode of mixing and irrigating with water, the growth rate of various nutrient elements in the soil after rejuvenation is smaller in comparative example 1 and comparative example 2, and the improvement effect is inferior to that of example 1.

The control group did not perform any rejuvenation treatment on the soil, the contents of available phosphorus and available potassium in the soil decreased, and the contents of organic matter and hydrolyzable nitrogen increased very slowly.

TABLE 7 detection of growth after rejuvenation of Pinus bungeana

TABLE 7

Combining the data of examples 13-24 and table 7, it can be seen that example 13 uses colorama, sorangium geotrichum and suillus luteus in a mass ratio of 4:3:3 as rejuvenating substrates, rejuvenates pinus albus with a diameter of 11cm in a manner of burying the annular groove, and after 24 months of growth, the ground diameter growth rate of the pinus albus is 17.2%, the tree height growth rate reaches 19.8%, the new branch length growth rate is 55.8%, and the new needle leaf length growth rate is 35.3%, which indicates that the colorama, sorangium geotrichum and suis luteus in a mass ratio of 4:3:3 are used as rejuvenating substrates, and rejuvenates the pinus albus in a manner of burying the annular groove, and can effectively promote the growth of root systems and needle bundles of the pinus albus and is beneficial to the rejuvenation effect after transplantation of the pinus albus.

In example 14, corn starch, chicken manure, and vermiculite are used as culture carriers of mycorrhizal fungi, and the prepared colorama, euglena lanuginosa, and suillus luteus are used as rejuvenation substrates, after rejuvenation, the growth rate of the ground diameter of pinus albus is 17.8%, and in addition, the growth rate of detection items such as tree height is obviously greater than that of example 13, which shows that the corn starch and chicken manure can further improve the soil improvement effect of mycorrhizal fungi.

In example 15, only the Pistacia lentiscus is used, the growth rate of the ground diameter of the pinus albus is 4.7%, in example 18, the Pistacia lentinus and the suillus luteus are used, the growth rate of the ground diameter of the pinus albus is 8.5%, in addition, in example 15, the growth effect of the tree height and the new needle length of the pinus albus is not as good as that of example 18, in example 15, the growth trend of the pinus albus is not as good as that of example 13 and example 14, and the Pistacia albus and the suis luteus are proved to be capable of cooperatively improving the growth of the root system, the ground diameter and the tree height of the pinus albus and promoting the growth of new branches.

In example 16, only the soil aeromonas campestris was used as the mycorrhizal fungus, and the tree height growth rate of the pinus bungeana was 6.2%, whereas in example 17 in which the soil aeromonas campestris and the suillus luteus were added, the tree height growth rate of the pinus bungeana was 7.7%, which indicates that the soil aeromonas campestris and the suillus luteus can cooperate to promote the trunk growth of the pinus bungeana.

In example 19, the growth rate of the diameter of the white bark pine after rejuvenation was 4.6% using boletus caerulea, which was less effective in promoting growth of white bark pine than in example 1, and less effective in promoting growth of white bark pine than in examples 15 and 16.

In examples 20 and 21, compared with example 14, when the slow release fertilizers prepared in preparation examples 15 and 16 were added to the rejuvenating substrate, respectively, the ground diameter, the tree height, the new shoot length, and the like of the white bark pine were increased, the ground diameter growth rate in example 20 was 22.9%, and the ground diameter growth rate in example 14 was 17.8%, which indicates that the rejuvenation effect of the white bark pine was further enhanced in example 20.

In examples 22 to 24, the slow release fertilizers prepared in preparation examples 17, 18 and 19 were used, respectively, and the ground diameter, the tree height, and the like of the lacebark pine were reduced as compared with example 8, which indicates that the slow release fertilizers prepared in preparation examples 17 to 19 had a poorer rejuvenation effect on the lacebark pine than those of preparation example 15.

In comparative example 3, the rejuvenating substrate was applied to only 0.5kg of white bark pine having a diameter of 11cm, and the growth of white bark pine was not good after rejuvenation, and the height and diameter of the tree were not good as in example 1.

In comparative example 4, the rejuvenation substrate was applied to the root of Pinus bungeana by mixing with water, and after rejuvenation, the root of Pinus bungeana was not well developed and the growth was not as good as that of example 1.

In the control group, the white bark pine was not treated by any rejuvenation treatment, and the growth rate of the ground diameter after 24 months of growth was 4.3%, and the growth rate of the tree height was 8.0%, and the white bark pine grew more slowly than those of examples 13 and 14.

The present embodiment is only for explaining the present application, and it is not limited to the present application, and those skilled in the art can make modifications of the present embodiment without inventive contribution as needed after reading the present specification, but all of them are protected by patent law within the scope of the claims of the present application.

Claims

1. A pine rejuvenation method is characterized in that: the method comprises the following steps:

s1, digging an annular ditch, namely digging the annular ditch on the ground surrounding the trunk, wherein the distance between the outer diameter of the annular ditch and the trunk is 1/2-2/3 of the weight and straight projection radius of the tree crown, the depth of the annular ditch is 15-20cm, and the width of the annular ditch is 5-10 cm;

s2, applying a rejuvenation substrate, namely filling the rejuvenation substrate into the annular ditch, compacting the annular ditch by using original soil, wherein the rejuvenation substrate is obtained by compounding a slow release fertilizer and mycorrhizal fungi in a mass ratio of 1-1.5:1, and the diameter of the pine is marked as H;

when the H is less than or equal to 10cm, the application amount of the rejuvenation substrate is 0.2-03 kg/plant:

when H is more than 15cm and less than or equal to 20cm, the application amount of the rejuvenation substrate is 1-1.5kg per plant;

when H is more than 20cm, the application amount of the rejuvenation substrate is 1.5-2 kg/plant;

the slow release fertilizer is prepared by the following method:

(1) uniformly stirring 3-5 parts by weight of mesoporous silica and 1-2 parts by weight of biochar, extruding, granulating, sterilizing to obtain carrier particles, and fermenting the carrier particles and the compound fertilizer at a mass ratio of 2-3:1 at 18-30 ℃ for 3-5 days to obtain a fermentation material;

(4) dissolving 1-2 parts by weight of polyvinyl alcohol, adding 0.8-1.2 parts by weight of earthworm fertilizer, uniformly mixing to form coating liquid, spraying the coating liquid on the particles obtained in the step (3), drying, mixing with the bacterial fertilizer particles obtained in the step (2), and preparing a slow release fertilizer;

the mycorrhizal fungi is prepared by the following method: sterilizing the substrate, uniformly mixing the sterilized substrate and a liquid culture medium according to the weight ratio of 1:2-3 to form a nutrient substrate, cutting mycorrhizal fungi hypha into small pieces, inoculating the small pieces on the nutrient substrate, and culturing the nutrient substrate at 25-28 ℃ in a ventilated and light-proof manner for 1 month, wherein the mass ratio of the mycorrhizal fungi hypha to the substrate is 1: 5-10;

the substrate is prepared by mixing vermiculite, corn flour and chicken manure in a mass ratio of 7:2-2.5: 0.5-1.

2. A pine rejuvenation method as defined in claim 1 wherein: when the pine tree is Pinus tabulaeformis, mycorrhizal fungi is mixture of Suillus viscosus, Mi Boehmeria perennea, Pleurotus ostreatus and Leucospora globosa, and the mass ratio of Suillus viscosus, Tricholoma palmatum, Pleurotus chromogenes and Leucospora globosa is 0.8-1.2:0.8-1.2: 0.8-1.2: 0.8-1.2.

3. A pine rejuvenation method as defined in claim 1 wherein: when the pine is white bark pine, the mycorrhizal fungi is a mixture of the Phaseolus versicolor, the Sucus luteus and the airborne vacuoloides, and the mass ratio of the mixture of the Phaseolus versicolor, the Sucus luteus and the airborne vacuoloides is 3.5-4.5:2.8-3.2: 2.8-3.2.

4. A pine rejuvenation method as defined in claim 1 wherein the sterilizing means of the matrix is: sterilizing at 80-90 deg.C for 2-3 hr.

5. A pine rejuvenation method as defined in claim 1 wherein: the compound fertilizer is selected from one or more of ferrous sulfate, ammonium phosphate and phosphogypsum.